##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936855_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 174311 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.878693828846142 31.0 31.0 34.0 30.0 34.0 2 31.81079220473751 31.0 31.0 34.0 30.0 34.0 3 31.953674753744743 31.0 31.0 34.0 30.0 34.0 4 35.69958292936189 37.0 35.0 37.0 33.0 37.0 5 35.64380905393234 37.0 35.0 37.0 33.0 37.0 6 35.800167516679956 37.0 35.0 37.0 35.0 37.0 7 35.768465558685335 37.0 35.0 37.0 33.0 37.0 8 35.78568765023435 37.0 35.0 37.0 33.0 37.0 9 37.31251039808159 39.0 37.0 39.0 34.0 39.0 10-11 37.248980270895125 39.0 37.0 39.0 33.5 39.0 12-13 37.22858282036131 39.0 37.0 39.0 33.5 39.0 14-15 38.082378622117936 40.0 37.0 41.0 33.0 41.0 16-17 38.05371433816569 39.5 37.0 41.0 33.0 41.0 18-19 38.23010882847325 40.0 37.0 41.0 33.5 41.0 20-21 38.379218752689155 40.0 38.0 41.0 34.0 41.0 22-23 38.29010217370103 40.0 37.5 41.0 34.0 41.0 24-25 38.067236146886884 40.0 37.0 41.0 33.5 41.0 26-27 37.76895606129274 39.5 36.5 41.0 33.0 41.0 28-29 37.65994400812341 39.0 36.0 41.0 33.0 41.0 30-31 37.542429909758994 39.0 36.0 41.0 33.0 41.0 32-33 37.2452742511947 39.0 35.0 41.0 33.0 41.0 34-35 36.95103292391186 39.0 35.0 41.0 32.5 41.0 36-37 36.6305281938604 38.5 35.0 40.5 31.5 41.0 38-39 36.31911353844565 38.0 35.0 40.0 31.0 41.0 40-41 36.26529019970053 39.0 35.0 40.0 31.0 41.0 42-43 35.83592257516737 38.5 35.0 40.0 30.0 41.0 44-45 35.27901853583538 38.0 35.0 40.0 28.0 41.0 46-47 34.67946658558553 38.0 35.0 40.0 23.5 41.0 48-49 34.03722082943704 38.0 34.0 40.0 19.0 41.0 50-51 33.66854931702532 38.0 34.0 40.0 11.5 41.0 52-53 33.554936292029765 38.0 34.0 40.0 8.0 41.0 54-55 33.3988159094951 38.0 33.5 40.0 4.5 41.0 56-57 32.99473068251574 38.0 33.0 40.0 2.0 41.0 58-59 32.73190733803374 38.0 33.0 40.0 2.0 41.0 60-61 32.15776686497123 37.0 31.5 39.5 2.0 41.0 62-63 31.908617356334368 36.0 31.5 39.0 2.0 40.5 64-65 31.556574169157425 36.0 31.0 39.0 2.0 40.0 66-67 30.951790191095228 35.5 30.5 38.5 2.0 40.0 68-69 30.40166139830533 35.0 30.0 37.5 2.0 39.0 70-71 29.711544308735537 34.5 29.0 37.0 2.0 39.0 72-73 29.274735960438527 34.0 29.0 36.5 2.0 38.5 74-75 28.943199224374823 34.0 29.0 36.0 2.0 37.0 76-77 27.59227759579143 32.5 27.0 34.5 2.0 36.0 78-79 28.25514454050519 34.0 29.0 35.0 2.0 36.0 80-81 27.9855488179174 34.0 28.0 35.0 2.0 36.0 82-83 27.594466212688815 33.5 26.5 35.0 2.0 36.0 84-85 27.216753389057487 33.0 26.0 35.0 2.0 35.0 86-87 27.118257023366283 33.5 26.0 35.0 2.0 35.0 88-89 26.698091342485558 33.0 25.0 35.0 2.0 35.0 90-91 26.16193470291605 33.0 24.5 35.0 2.0 35.0 92-93 25.757074998135515 33.0 23.5 35.0 2.0 35.0 94-95 25.19552982886909 32.0 19.5 34.0 2.0 35.0 96-97 24.45018386676687 32.0 12.0 34.0 2.0 35.0 98-99 23.90483388885383 32.0 2.0 34.0 2.0 35.0 100-101 21.161099987952568 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4197446256842632 1101 2 0.3571392027360929 1101 3 0.40480298161056893 1101 4 0.147750660921119 1101 5 0.4979043094087032 1101 6 0.5442386565240085 1101 7 0.4533942080164337 1101 8 0.44605951257147325 1101 9 0.603832181774969 1101 10-11 0.7061476824094157 1101 12-13 0.39008621478149763 1101 14-15 0.7853793350537686 1101 16-17 0.8744342045471782 1101 18-19 1.0638824537168148 1101 20-21 0.7759376180370481 1101 22-23 0.8162812612219739 1101 24-25 0.9718728164983688 1101 26-27 0.8085741520395331 1101 28-29 1.0180015642173288 1101 30-31 1.2709117309829452 1101 32-33 1.2793635193998227 1101 34-35 1.4927998690754336 1101 36-37 1.387164694509714 1101 38-39 1.6631335361382895 1101 40-41 1.5863066348148962 1101 42-43 1.9347985309890774 1101 44-45 1.9164678432535922 1101 46-47 1.8866116615524078 1101 48-49 1.6249211008250697 1101 50-51 1.9982403426329256 1101 52-53 1.6964993448961607 1101 54-55 1.5393988490966208 1101 56-57 1.9678255483030611 1101 58-59 2.0439842131170067 1101 60-61 1.7363687904176004 1101 62-63 1.8821085957492336 1101 64-65 2.0930184810559602 1101 66-67 1.9486889540827548 1101 68-69 1.7476845613117007 1101 70-71 1.6874077299937191 1101 72-73 2.1359984126469946 1101 74-75 1.9852456836560215 1101 76-77 1.8819082813592694 1101 78-79 1.9644551525141516 1101 80-81 2.0184988269547866 1101 82-83 2.1828300400442373 1101 84-85 2.0935437527019936 1101 86-87 1.8725773423872027 1101 88-89 1.7282387481641805 1101 90-91 1.8587311367886485 1101 92-93 2.434620959321233 1101 94-95 2.482452040867372 1101 96-97 2.8463033613384816 1101 98-99 3.258985220251809 1101 100-101 2.343687618015462 1102 1 0.11553257049181909 1102 2 -0.18156813204967293 1102 3 0.15207967006372414 1102 4 -0.12908303552041644 1102 5 0.2959435250949767 1102 6 0.149176928128945 1102 7 0.23618287904039903 1102 8 0.3982744726295735 1102 9 0.5055751011649505 1102 10-11 0.35320650593882874 1102 12-13 0.2903403905258628 1102 14-15 0.4937308237974136 1102 16-17 0.6483993461593798 1102 18-19 0.3583632089818849 1102 20-21 0.4626841685090852 1102 22-23 -0.017741977703224165 1102 24-25 -0.0158541261305416 1102 26-27 0.17513188624432274 1102 28-29 0.17152371381790488 1102 30-31 0.2612893635174416 1102 32-33 0.46618269151311154 1102 34-35 0.08807946239424069 1102 36-37 0.030120395308550485 1102 38-39 0.12460775545564928 1102 40-41 0.670947157690712 1102 42-43 1.0021914140682355 1102 44-45 1.3654511402761003 1102 46-47 1.7925666361348362 1102 48-49 1.9315296701787403 1102 50-51 2.169700037622036 1102 52-53 2.1215175003064743 1102 54-55 2.040851281921597 1102 56-57 1.859728235303784 1102 58-59 1.9059304731024795 1102 60-61 1.104850998115495 1102 62-63 1.2921666930622386 1102 64-65 0.8972668470690373 1102 66-67 0.8663360129062845 1102 68-69 0.9257528256544703 1102 70-71 0.5242995237482582 1102 72-73 0.8200942732134457 1102 74-75 0.7158193946218852 1102 76-77 1.172649022100007 1102 78-79 1.0724072222309253 1102 80-81 1.2376346294239227 1102 82-83 1.5418351235591246 1102 84-85 1.1993171731812957 1102 86-87 1.4521343503755872 1102 88-89 1.497737658839565 1102 90-91 1.2422823350457293 1102 92-93 1.4235824698513682 1102 94-95 1.7935994627991079 1102 96-97 1.3438705363566363 1102 98-99 1.8916286479932722 1102 100-101 1.5218285039994832 1103 1 0.21018195566961495 1103 2 -0.009883186885168982 1103 3 0.06833091632494259 1103 4 0.2932831991090268 1103 5 0.33123764274203893 1103 6 0.36386619366566464 1103 7 0.43299245032653744 1103 8 0.24842402397553798 1103 9 0.47137769338827695 1103 10-11 0.5197070044433119 1103 12-13 0.2955580708183234 1103 14-15 0.5581874539070881 1103 16-17 0.7876482660663271 1103 18-19 0.7389713844554606 1103 20-21 0.25736678731932017 1103 22-23 0.6303637052479161 1103 24-25 1.0215170935437712 1103 26-27 1.038957077337706 1103 28-29 0.4696127799528327 1103 30-31 0.72921890821668 1103 32-33 0.9777313787846253 1103 34-35 1.0316155208854383 1103 36-37 1.1619083647336055 1103 38-39 1.1117629565191223 1103 40-41 0.4657793278469171 1103 42-43 -0.06106880527373448 1103 44-45 -0.35796195377508155 1103 46-47 -0.2311954110569232 1103 48-49 -0.5446750489970711 1103 50-51 -0.33211119534573186 1103 52-53 -0.30470383568555093 1103 54-55 0.036364741542733725 1103 56-57 0.022805669667363304 1103 58-59 0.376585175661468 1103 60-61 0.36463830286790966 1103 62-63 0.05311717494990731 1103 64-65 -0.3382432891888598 1103 66-67 -0.09085906851611725 1103 68-69 -0.03333657281677915 1103 70-71 -0.1373987148692244 1103 72-73 0.2549563536388355 1103 74-75 -0.21040194137013657 1103 76-77 -0.3872379835497064 1103 78-79 0.08294228371314816 1103 80-81 0.4848097706668604 1103 82-83 0.6312502116282523 1103 84-85 0.8550001243278551 1103 86-87 0.5395055700498972 1103 88-89 0.7905425774171633 1103 90-91 1.0533325178307074 1103 92-93 1.050335543962369 1103 94-95 1.4456765648263605 1103 96-97 1.338038033864116 1103 98-99 0.981259754673232 1103 100-101 1.1920345525205889 1104 1 0.34768031330987625 1104 2 0.10146880365483923 1104 3 0.3948977275192682 1104 4 0.29410437898026487 1104 5 -0.017599566560285496 1104 6 0.14336297464057424 1104 7 0.31149067657801766 1104 8 0.29109253008511615 1104 9 0.06528782346317286 1104 10-11 0.38638024054895226 1104 12-13 0.5626386121400984 1104 14-15 0.7041275406236807 1104 16-17 0.6656702079924841 1104 18-19 0.5919473403088276 1104 20-21 0.29808088533535226 1104 22-23 0.12090371313124848 1104 24-25 0.3867450530760266 1104 26-27 0.17205391086812227 1104 28-29 0.3006960804389678 1104 30-31 0.6114945618758583 1104 32-33 0.5036313919235056 1104 34-35 0.2674912271001233 1104 36-37 0.31305473183385146 1104 38-39 0.6685886036088604 1104 40-41 0.5549758854608982 1104 42-43 0.6052693894444374 1104 44-45 0.8716070910285012 1104 46-47 0.8212944063126741 1104 48-49 0.11709738563700967 1104 50-51 0.07780537277935196 1104 52-53 -0.40994624798353385 1104 54-55 0.14293746523213002 1104 56-57 0.11116462381267667 1104 58-59 -0.2617507854474539 1104 60-61 -0.3403610401881991 1104 62-63 0.07404083806908446 1104 64-65 -0.11757583418951612 1104 66-67 -0.10945058080096715 1104 68-69 -0.09917877489272087 1104 70-71 0.6719397426265061 1104 72-73 0.46373312410263523 1104 74-75 0.655285878890016 1104 76-77 0.576137394193033 1104 78-79 0.2279133781816789 1104 80-81 0.740541606168108 1104 82-83 0.830583413572807 1104 84-85 0.9037289378871769 1104 86-87 0.7593846923728478 1104 88-89 1.0626487395509159 1104 90-91 1.4583220067283555 1104 92-93 1.3483088720401533 1104 94-95 1.4417184778469867 1104 96-97 1.1303616444277758 1104 98-99 1.7244603853393734 1104 100-101 1.4140651661061874 1105 1 0.27124968879981637 1105 2 -0.2673579535962709 1105 3 0.16364609438626232 1105 4 0.08525933063953062 1105 5 0.3368556202701285 1105 6 0.2896263663311913 1105 7 0.33063099536558127 1105 8 0.14691954863750567 1105 9 0.3936782075760519 1105 10-11 0.39715891836447526 1105 12-13 0.2669762133943365 1105 14-15 7.698191708485069E-4 1105 16-17 0.33313166605363165 1105 18-19 0.3679468177062688 1105 20-21 0.49781305152296795 1105 22-23 -0.0015159367250348055 1105 24-25 0.24250674630832236 1105 26-27 -0.06429309723221621 1105 28-29 0.050010164577983574 1105 30-31 0.20197013020613497 1105 32-33 0.4339619380431827 1105 34-35 0.5118732495720337 1105 36-37 0.35966434772097244 1105 38-39 0.48280339326132804 1105 40-41 0.11033909424062216 1105 42-43 0.9820658357470222 1105 44-45 0.7937393094763721 1105 46-47 0.7378739725525492 1105 48-49 0.9493419636354403 1105 50-51 0.9999049285671759 1105 52-53 0.617882338376539 1105 54-55 0.2612742825825407 1105 56-57 0.7473025908887152 1105 58-59 0.929162462527465 1105 60-61 0.8418966028742503 1105 62-63 0.794579118706654 1105 64-65 0.9634928880472238 1105 66-67 0.9065541226220795 1105 68-69 0.7338823696068886 1105 70-71 0.7269763248057579 1105 72-73 0.3752826406952714 1105 74-75 0.7571280529166664 1105 76-77 0.7771434041224801 1105 78-79 0.9627905665799013 1105 80-81 1.2910054159407771 1105 82-83 1.0399184018142478 1105 84-85 0.8882794996849306 1105 86-87 0.9599334250616423 1105 88-89 0.8385175663081696 1105 90-91 1.1868626390774537 1105 92-93 1.247805867075428 1105 94-95 1.2396999254560832 1105 96-97 1.5410945945192296 1105 98-99 1.8072928912186548 1105 100-101 1.4030578496703363 1106 1 0.06037090317588323 1106 2 -0.25761020450779526 1106 3 -0.018547521210134477 1106 4 0.06635911484966073 1106 5 -0.09922593341690344 1106 6 0.10281931223490659 1106 7 0.17291836793794602 1106 8 0.2137399965874991 1106 9 0.05146524530207586 1106 10-11 0.10680993406504768 1106 12-13 0.22454374074012406 1106 14-15 0.7550670139025968 1106 16-17 0.30753452340908893 1106 18-19 0.3588501587286643 1106 20-21 0.19256340497187807 1106 22-23 0.116052334723733 1106 24-25 0.12919846444211203 1106 26-27 -0.1375872411417376 1106 28-29 -0.027599776637885043 1106 30-31 0.07098940247341545 1106 32-33 0.21988350958868352 1106 34-35 0.23272301517958738 1106 36-37 0.37240711131945403 1106 38-39 0.464907716499674 1106 40-41 0.7470866200981945 1106 42-43 0.9712019321827725 1106 44-45 0.8700284678958923 1106 46-47 0.39560520255477627 1106 48-49 0.33406180886202463 1106 50-51 0.04821581479382786 1106 52-53 0.26993978312689393 1106 54-55 0.29542860954138916 1106 56-57 0.9236549980618705 1106 58-59 0.8280574695964376 1106 60-61 0.9589998099581081 1106 62-63 0.7154623689484829 1106 64-65 0.8310611594959916 1106 66-67 0.8460399474602376 1106 68-69 1.1144166355492935 1106 70-71 0.48625900952932355 1106 72-73 0.6862804827966151 1106 74-75 0.13907611452839674 1106 76-77 -0.07768210372780615 1106 78-79 0.43434723002212294 1106 80-81 0.42472072213915624 1106 82-83 0.6258436938746677 1106 84-85 0.8946229776222765 1106 86-87 0.5776505171071804 1106 88-89 1.0519449046517977 1106 90-91 0.8614291990893577 1106 92-93 0.5311788859012303 1106 94-95 1.023556611221391 1106 96-97 1.5052846384213048 1106 98-99 1.4654891855857954 1106 100-101 1.2773602531229749 1107 1 0.2904657255720302 1107 2 0.47210833853856116 1107 3 0.19920533851292177 1107 4 0.3786709751747708 1107 5 0.10964673365113242 1107 6 0.274176928128945 1107 7 0.16719892182114648 1107 8 -0.17164531346668355 1107 9 0.37950558244837396 1107 10-11 0.37092040968214235 1107 12-13 0.6113965402584896 1107 14-15 0.6333030163107836 1107 16-17 0.4234661910791644 1107 18-19 0.6823939576449831 1107 20-21 0.6079381792042682 1107 22-23 0.48533288860693347 1107 24-25 0.45994801290689935 1107 26-27 0.01553964025502097 1107 28-29 -0.1486300294976033 1107 30-31 0.23973187688642383 1107 32-33 0.35154365407995414 1107 34-35 0.25896849982739667 1107 36-37 0.24315515466683024 1107 38-39 0.31321069663211887 1107 40-41 0.22582683683510396 1107 42-43 0.5998518418757257 1107 44-45 0.596567450436531 1107 46-47 0.23758668961076523 1107 48-49 0.20853501777231997 1107 50-51 0.2809634066059914 1107 52-53 0.6088169655471134 1107 54-55 0.21230850117293443 1107 56-57 0.398030374341225 1107 58-59 0.3915922378083607 1107 60-61 0.617752067634207 1107 62-63 0.2634568000141151 1107 64-65 0.5611371679246453 1107 66-67 0.7327798631736613 1107 68-69 0.7589079058683765 1107 70-71 0.5991992563685784 1107 72-73 0.755421813320396 1107 74-75 0.6519824962261644 1107 76-77 0.8129899311909163 1107 78-79 0.886277543086539 1107 80-81 1.382520993060286 1107 82-83 1.1185062465537747 1107 84-85 0.8753198469780834 1107 86-87 0.9722546712311981 1107 88-89 1.401113327288762 1107 90-91 1.892716827024337 1107 92-93 1.793803058086663 1107 94-95 1.918198393280388 1107 96-97 2.161083103201559 1107 98-99 2.0634535143034327 1107 100-101 1.6803178088123225 1108 1 0.051502820660850546 1108 2 -0.20820775341546138 1108 3 -0.20119957266784994 1108 4 -0.07979297466849289 1108 5 -0.053819828522328805 1108 6 -0.08645525577910007 1108 7 0.14368795003431245 1108 8 -0.11247603446982168 1108 9 0.08914508088097506 1108 10-11 0.19752699275424135 1108 12-13 0.3689069895792798 1108 14-15 0.11083697660336611 1108 16-17 -0.05596170860735583 1108 18-19 0.018437583559297366 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-1.3459857223957883 2316 88-89 -1.435373317509736 2316 90-91 -1.3071718796298342 2316 92-93 -1.8514933336659176 2316 94-95 -1.506825036897677 2316 96-97 -0.9921209736494241 2316 98-99 -2.317316167046144 2316 100-101 -1.676230925958066 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 8.0 6 43.0 7 55.0 8 90.0 9 163.0 10 333.0 11 576.0 12 936.0 13 1225.0 14 1570.0 15 2214.0 16 3011.0 17 3907.0 18 3163.0 19 1478.0 20 1249.0 21 1160.0 22 1298.0 23 1354.0 24 1607.0 25 1817.0 26 2053.0 27 2535.0 28 3196.0 29 3998.0 30 4824.0 31 5805.0 32 7429.0 33 9324.0 34 12903.0 35 18512.0 36 24726.0 37 32709.0 38 18250.0 39 788.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.77571852353383 21.204262082498953 16.815372874840058 14.204646519127156 2 14.362672541392724 27.611212465434352 34.31035076245224 23.715764230720687 3 28.0691186341497 24.321750454652367 35.555249585506196 12.053881325691732 4 11.07732730579252 19.605762114840715 43.34092512807568 25.975985451291084 5 8.153957362829015 42.95557289395782 36.57433967459899 12.316130068614177 6 21.971074688344398 40.47822570004188 17.531882669481558 20.01881694213217 7 19.469224546930487 40.49314156880518 22.650894091594907 17.386739792669424 8 23.802284422669825 25.12750199356323 21.810442255508832 29.25977132825811 9 19.365387152847497 23.925053496337007 29.449088124100026 27.260471226715467 10-11 24.070483216779202 25.1151677174705 27.97270396016316 22.84164510558714 12-13 30.446418583037644 26.77708329150161 26.464134198078703 16.31236392738204 14-15 22.932861379947337 28.585975641238935 34.51532031828169 13.965842660532038 16-17 15.39088181468754 31.35917985669292 33.751455731422574 19.498482597196965 18-19 14.893781803787483 34.37075112872968 34.698613397892274 16.03685366959056 20-21 18.961249071314445 26.21173743800396 32.54687889940363 22.28013459127796 22-23 16.489492917830773 26.9882566217852 35.66556327483635 20.85668718554767 24-25 22.779519630988474 27.387000261038935 33.12067743906462 16.712802668907965 26-27 20.12695881153243 23.090956551172205 41.698769710025616 15.083314927269756 28-29 15.008462178365509 33.54063279882964 35.89828174751155 15.552623275293307 30-31 17.71689235712646 26.973835207711726 34.756713334863434 20.55255910029837 32-33 17.989891514494374 27.666959250526364 37.416599257640165 16.926549977339093 34-35 21.556343441992816 26.49593244065035 34.475864284648836 17.471859832707995 36-37 19.09553792974857 24.861376055994608 39.82472999526685 16.218356018989972 38-39 17.601955199091247 28.797305864163004 37.41613096621143 16.184607970534323 40-41 17.324765852469127 30.533698598700536 35.0697781156323 17.07175743319803 42-43 16.027663675792276 30.681609564678613 31.39213346490121 21.8985932946279 44-45 19.950719133482878 25.752264682428127 32.6054031106037 21.69161307348529 46-47 23.23841650388244 29.93910292265813 32.64203820194423 14.180442371515198 48-49 20.269289572343606 27.88185450675662 34.04958393212113 17.799271988778646 50-51 13.713172757808623 30.109775372108967 38.29688974559765 17.880162124484755 52-53 18.97273791220139 33.73224407370861 28.841189159418963 18.45382885467104 54-55 16.227682669359282 27.127030934459334 32.80045208371581 23.844834312465576 56-57 21.261832367620904 27.81567322586197 30.18128621421605 20.741208192301073 58-59 26.036159819172223 27.1950180569619 28.956539947621902 17.81228217624398 60-61 19.839480462856194 28.75238800061959 35.9106529209622 15.497478615562018 62-63 17.368381800345357 29.230513277991637 34.687426496319794 18.713678425343208 64-65 16.40487404696204 34.41377767323921 31.414827520925243 17.766520758873508 66-67 19.395274524483607 29.166343966657198 28.013802955071554 23.424578553787637 68-69 16.551451141924492 28.855034966238506 31.93401449134019 22.659499400496813 70-71 22.566275220726173 29.560096608934604 28.975509290865176 18.898118879474044 72-73 20.351842396635895 24.808245033302544 36.76216647256914 18.077746097492415 74-75 16.409270839309276 35.15288853192588 30.806895760426826 17.63094486833802 76-77 18.25918839764901 28.749150218433307 30.019304742426545 22.97235664149114 78-79 18.373768723717955 29.989501523139676 31.938603989421203 19.698125763721166 80-81 21.3019245167194 28.001158898128836 30.463817421910633 20.23309916324113 82-83 17.985537219715507 27.92360714705739 34.77636043933349 19.31449519389362 84-85 17.55059306111326 30.113448933637393 32.06086944050027 20.275088564749076 86-87 17.08460263095996 31.642944426277474 30.708970850615856 20.563482092146703 88-89 16.823523674842736 30.98167340276921 27.93909683637438 24.25570608601367 90-91 18.62389636913333 25.306492418722858 31.37380888182616 24.69580233031765 92-93 21.17823889484886 29.013659493663624 32.16463676991125 17.64346484157626 94-95 19.02323726912607 27.677334411868475 32.72723100444322 20.57219731456223 96-97 13.951333690934803 28.897162805240107 35.83855154194121 21.31295196188389 98-99 18.025512238359003 31.14019109796711 28.212336174082157 22.62196048959173 100-101 16.07562367155163 27.153843285401624 31.941850892516516 24.82868215053023 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3449.0 1 2055.0 2 599.0 3 487.0 4 349.5 5 292.5 6 433.5 7 766.0 8 1034.5 9 1008.5 10 741.5 11 477.5 12 389.0 13 366.0 14 357.5 15 362.5 16 382.0 17 435.5 18 489.5 19 520.5 20 607.5 21 735.5 22 836.5 23 931.5 24 1100.0 25 1439.0 26 2222.0 27 3346.5 28 3667.0 29 3773.5 30 4040.5 31 4189.5 32 4246.5 33 4048.5 34 4784.5 35 5708.5 36 6004.0 37 5687.0 38 4078.5 39 2638.5 40 2192.5 41 1855.0 42 1622.0 43 1506.5 44 1427.0 45 1516.5 46 2763.5 47 11405.0 48 28828.0 49 27456.0 50 10179.5 51 2705.5 52 884.5 53 459.5 54 344.0 55 262.0 56 198.0 57 186.5 58 172.5 59 154.5 60 172.0 61 175.0 62 142.0 63 91.0 64 52.0 65 45.0 66 35.5 67 31.5 68 27.5 69 16.0 70 12.5 71 10.0 72 6.0 73 4.5 74 3.0 75 2.5 76 2.5 77 1.5 78 2.0 79 1.5 80 0.5 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01721061780381043 2 0.0028684363006350718 3 0.0011473745202540287 4 0.0 5 0.0017210617803810433 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 8.605308901905216E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0031552799306985794 22-23 0.0 24-25 0.004302654450952607 26-27 0.0014342181503175359 28-29 0.0034421235607620866 30-31 0.017784305063937446 32-33 0.0011473745202540287 34-35 0.0028684363006350718 36-37 0.0048763417110796224 38-39 0.003728967190825593 40-41 0.0048763417110796224 42-43 0.0017210617803810433 44-45 0.0022947490405080574 46-47 2.868436300635072E-4 48-49 2.868436300635072E-4 50-51 2.868436300635072E-4 52-53 0.0017210617803810433 54-55 0.0040158108208891005 56-57 5.736872601270144E-4 58-59 8.605308901905216E-4 60-61 0.0011473745202540287 62-63 0.0 64-65 0.0 66-67 2.868436300635072E-4 68-69 0.0 70-71 0.0 72-73 0.0 74-75 5.736872601270144E-4 76-77 8.605308901905216E-4 78-79 0.0 80-81 0.004302654450952607 82-83 8.605308901905216E-4 84-85 0.0020079054104445502 86-87 0.0011473745202540287 88-89 8.605308901905216E-4 90-91 0.0 92-93 0.0 94-95 2.868436300635072E-4 96-97 8.605308901905216E-4 98-99 0.0014342181503175359 100-101 2.868436300635072E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 174311.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.888589934083335 #Duplication Level Percentage of deduplicated Percentage of total 1 77.61336102457598 15.436203108237576 2 10.352486442829122 4.117927153191709 3 3.8421599169262723 2.2924542914675494 4 1.8028152763355256 1.434218150317536 5 1.1278412368755046 1.121558593548313 6 0.8134302526825892 0.9706788441349083 7 0.4874812507211261 0.678672028730258 8 0.3721010730356525 0.5920452524510788 9 0.32017999307718936 0.5731135728668874 >10 2.3624091381100727 10.050427110165165 >50 0.4326756663205262 5.954300072858282 >100 0.3749855774777893 15.550366873002853 >500 0.028845044421368413 4.011795010068211 >1k 0.0634590977270105 28.342445399315018 >5k 0.005769008884273681 8.873794539644658 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9806 5.6255772728055025 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5662 3.2482172668391556 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4512 2.5884769176930886 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3506 2.011347534005312 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3271 1.876531027875464 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2983 1.7113090969588838 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2869 1.645908749304404 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2804 1.6086190773961482 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2793 1.6023085175347511 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2711 1.555266162204336 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2461 1.4118443471725823 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2334 1.3389860651364514 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2325 1.3338228797953084 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1929 1.1066427247850108 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1905 1.0928742305419623 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1903 1.0917268560217084 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1707 0.9792841530368135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1687 0.9678104078342732 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1608 0.9224891142842392 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1412 0.8100464112993443 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1255 0.719977511459403 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1170 0.6712140943486068 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1143 0.6557245383251774 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1116 0.640234982301748 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 939 0.5386923372592665 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 919 0.5272185920567262 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 856 0.4910762946687243 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 845 0.48476573480732715 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 664 0.38092834072433757 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 606 0.3476544796369707 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 572 0.32814911279265224 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 553 0.3172490548502389 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 536 0.3074963714280797 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 503 0.2885646918438882 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 477 0.27364882308058586 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 467 0.2679119504793157 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 465 0.26676457595906167 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 454 0.2604540160976645 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 453 0.2598803288375375 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 453 0.2598803288375375 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 435 0.24955395815525125 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 433 0.2484065836349972 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 430 0.24668552185461617 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 426 0.24439077281410812 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 407 0.23349071487169482 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 403 0.2311959658311868 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 392 0.22488540596978962 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 390 0.2237380314495356 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 376 0.2157064098077574 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 356 0.2042326646052171 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 344 0.19734841748369295 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 334 0.1916115448824228 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 332 0.19046417036216878 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 331 0.18989048310204176 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 316 0.18128517420013654 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 311 0.17841673789950147 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 308 0.17669567611912043 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 295 0.16923774173746925 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 287 0.1646482436564531 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 284 0.16292718187607208 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 277 0.15891137105518297 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 268 0.15374818571403984 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 264 0.1514534366735318 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 256 0.14686393859251567 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 254 0.14571656407226166 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 252 0.14456918955200762 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 250 0.14342181503175358 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 249 0.14284812777162656 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 248 0.14227444051149957 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 246 0.14112706599124553 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 244 0.1399796914709915 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 234 0.13424281886972136 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 234 0.13424281886972136 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 233 0.13366913160959434 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 230 0.13194806982921328 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 224 0.12850594626845122 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 224 0.12850594626845122 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 221 0.12678488448807015 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 221 0.12678488448807015 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 219 0.12563750996781614 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 215 0.12334276092730809 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 208 0.11932695010641897 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 208 0.11932695010641897 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 208 0.11932695010641897 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 207 0.11875326284629198 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 201 0.1153111392855299 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 200 0.11473745202540286 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 200 0.11473745202540286 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 198 0.11359007750514885 No Hit GCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTA 194 0.11129532846464078 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 193 0.11072164120451376 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 189 0.10842689216400572 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 189 0.10842689216400572 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 189 0.10842689216400572 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 189 0.10842689216400572 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 185 0.10613214312349765 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 185 0.10613214312349765 No Hit TGATACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCA 180 0.10326370682286258 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 178 0.10211633230260855 No Hit TCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAA 178 0.10211633230260855 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 5.736872601270144E-4 0.0 10-11 0.0 0.0 0.0 5.736872601270144E-4 0.0 12-13 0.0 0.0 0.0 5.736872601270144E-4 0.0 14-15 0.0 0.0 0.0 0.0011473745202540287 0.0 16-17 0.0 0.0 0.0 0.0028684363006350718 0.0 18-19 0.0 0.0 0.0 0.003442123560762086 0.0 20-21 0.0 0.0 0.0 0.0040158108208891005 0.0 22-23 0.0 0.0 0.0 0.005163185341143129 0.0 24-25 0.0 0.0 0.0 0.008605308901905216 0.0 26-27 0.0 0.0 0.0 0.010900057942413274 0.0 28-29 0.0 0.0 0.0 0.02237380314495356 0.0 30-31 0.0 0.0 0.0 0.0438870753997166 0.0 32-33 0.0 0.0 0.0 0.07429250018644837 0.0 34-35 0.0 0.0 0.0 0.1078532049038787 0.0 36-37 0.0 0.0 0.0 0.15001921852321426 0.0 38-39 0.0 0.0 0.0 0.20996953720648726 0.0 40-41 0.0 0.0 0.0 0.26676457595906167 0.0 42-43 0.0 0.0 0.0 0.31438061854960386 0.0 44-45 0.0 0.0 0.0 0.3476544796369707 0.0 46-47 0.0 0.0 0.0 0.39412314770725887 0.0 48-49 0.0 0.0 0.0 0.4273970087946257 0.0 50-51 0.0 0.0 0.0 0.46267877529243706 0.0 52-53 0.0 0.0 0.0 0.5435686789703461 0.0 54-55 0.0 0.0 0.0 0.680966777770766 0.0 56-57 0.0 0.0 0.0 0.8926573767576343 0.0 58-59 0.0 0.0 0.0 1.0879978888308828 0.0 60-61 0.0 0.0 0.0 1.3300939126044828 0.0 62-63 0.0 0.0 0.0 1.4689262295552203 0.0 64-65 0.0 0.0 0.0 1.635582378622118 0.0 66-67 0.0 0.0 0.0 1.9645920223049607 0.0 68-69 0.0 0.0 0.0 2.4373103246496206 0.0 70-71 0.0 0.0 0.0 2.8529467446116423 0.0 72-73 0.0 0.0 0.0 3.278622691625887 0.0 74-75 0.0 0.0 0.0 3.7435962159588323 0.0 76-77 0.0 0.0 0.0 4.086948041144851 0.0 78-79 0.0 0.0 0.0 4.502010773846745 0.0 80-81 0.0 0.0 0.0 4.849378409853652 0.0 82-83 0.0 0.0 0.0 5.221988285306148 0.0 84-85 0.0 0.0 0.0 5.913281433759201 0.0 86-87 0.0 0.0 0.0 6.696938231092702 0.0 88-89 0.0 0.0 0.0 7.655856486395007 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTCATC 20 0.0020725315 71.23774 8 TATTACT 20 0.0020725315 71.23774 2 TGGGTCA 35 3.4083478E-6 67.84546 6 GGTCAAT 30 1.2992494E-4 63.322437 8 TGGGACT 35 2.783735E-4 54.27637 4 GGGTCAA 40 5.3800957E-4 47.491825 7 CCTTTAG 130 0.0 43.838608 1 AGGCATT 115 5.4569682E-11 41.297237 6 CTTTAGG 145 0.0 39.30358 2 TTAGGCA 135 7.2759576E-12 38.697044 4 GGCATTC 135 7.2759576E-12 38.697044 7 ATGGGAC 65 1.3249075E-4 36.532173 3 TAGGCAT 130 2.0372681E-10 36.532173 5 TGGGAAC 55 0.0025758243 34.53951 6 GCATTCC 155 3.6379788E-11 33.703876 8 CATTCCG 165 7.4578566E-11 31.661215 9 CGCAGAA 115 3.845038E-6 28.908066 9 ATGGGAT 185 2.8558134E-10 28.238382 5 GATAGTG 35 0.008378997 27.145973 22-23 GTTGATC 70 5.7953E-7 27.138186 94-95 >>END_MODULE