##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936854_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 166153 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.933603365572694 33.0 31.0 34.0 30.0 34.0 2 31.97571515410495 33.0 31.0 34.0 30.0 34.0 3 31.96485769140491 33.0 31.0 34.0 30.0 34.0 4 35.49887754058007 37.0 35.0 37.0 33.0 37.0 5 35.55132919658387 37.0 35.0 37.0 33.0 37.0 6 35.740275529180934 37.0 35.0 37.0 35.0 37.0 7 35.73595421087793 37.0 35.0 37.0 35.0 37.0 8 35.712909186111595 37.0 35.0 37.0 35.0 37.0 9 37.27872503054414 39.0 37.0 39.0 34.0 39.0 10-11 37.119040884004505 39.0 37.0 39.0 33.5 39.0 12-13 37.13635624996238 39.0 37.0 39.0 34.0 39.0 14-15 38.09487640909283 40.0 38.0 41.0 33.0 41.0 16-17 38.1093419920194 40.0 38.0 41.0 33.5 41.0 18-19 38.16298532075858 40.0 38.0 41.0 34.0 41.0 20-21 38.07593904413402 40.0 38.0 41.0 34.0 41.0 22-23 37.981757777470165 40.0 37.5 41.0 33.5 41.0 24-25 37.67412866454413 40.0 36.5 41.0 32.5 41.0 26-27 37.66316587723363 39.5 36.5 41.0 33.0 41.0 28-29 37.69218431204973 40.0 37.0 41.0 33.0 41.0 30-31 37.5947289546382 39.5 36.5 41.0 33.0 41.0 32-33 37.195157475338995 39.0 35.0 41.0 33.0 41.0 34-35 36.89743489434437 39.0 35.0 41.0 32.0 41.0 36-37 36.562938977929974 38.5 35.0 40.5 31.0 41.0 38-39 36.282622041130765 38.0 35.0 40.0 31.0 41.0 40-41 36.254048978953136 39.0 35.0 40.0 31.0 41.0 42-43 35.87595770163644 38.5 35.0 40.0 30.0 41.0 44-45 35.42755171438373 38.0 35.0 40.0 29.5 41.0 46-47 34.70116699668378 38.0 35.0 40.0 24.0 41.0 48-49 34.03929510752138 38.0 34.0 40.0 19.5 41.0 50-51 33.172217775183114 37.5 33.5 39.5 14.0 40.5 52-53 33.46077711506864 38.0 33.5 39.5 11.5 40.5 54-55 33.846226068743874 38.0 34.5 40.0 8.0 41.0 56-57 33.61743092210192 38.0 34.0 40.0 4.5 41.0 58-59 33.41029352464295 38.0 34.0 40.0 2.0 41.0 60-61 32.8343213784885 38.0 33.0 40.0 2.0 41.0 62-63 32.63282637087504 37.0 33.0 40.0 2.0 41.0 64-65 32.30714762899255 37.0 32.5 39.5 2.0 41.0 66-67 31.652079709665188 36.0 31.5 39.0 2.0 40.5 68-69 31.1516373463013 36.0 31.0 38.5 2.0 40.0 70-71 30.435634625917075 35.0 30.5 37.5 2.0 39.0 72-73 29.954069442020305 35.0 29.5 37.0 2.0 39.0 74-75 29.6048852563601 35.0 29.5 36.5 2.0 38.0 76-77 29.188263227266432 34.5 29.0 36.0 2.0 37.0 78-79 28.663096663918196 34.0 29.0 35.5 2.0 37.0 80-81 28.10383201025561 34.0 28.0 35.0 2.0 36.5 82-83 27.616458926411198 34.0 26.5 35.0 2.0 36.0 84-85 27.275150012338024 34.0 26.0 35.0 2.0 36.0 86-87 27.219400191389866 34.0 26.0 35.0 2.0 35.0 88-89 26.668772155784126 34.0 25.0 35.0 2.0 35.0 90-91 26.174191859310394 33.0 24.0 35.0 2.0 35.0 92-93 25.778195398217306 33.0 21.5 35.0 2.0 35.0 94-95 25.23803662889024 33.0 18.5 35.0 2.0 35.0 96-97 24.728746998248603 33.0 6.0 35.0 2.0 35.0 98-99 24.278905587019192 33.0 2.0 35.0 2.0 35.0 100-101 22.174814779149337 30.0 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3489652766048188 1101 2 0.4189667956236889 1101 3 0.25398569375689206 1101 4 0.3926147176156576 1101 5 0.2988763820572231 1101 6 -0.04487141367892633 1101 7 0.15776049865063158 1101 8 0.2123286294657234 1101 9 0.05798878317850864 1101 10-11 0.18844831222135383 1101 12-13 0.44240494512385453 1101 14-15 0.5465319453846647 1101 16-17 0.5730405007023478 1101 18-19 0.7796868916873549 1101 20-21 0.8219224191072172 1101 22-23 0.9074734888019194 1101 24-25 0.8982753152729472 1101 26-27 1.1260456711573283 1101 28-29 1.068877418570203 1101 30-31 1.0895960274808658 1101 32-33 1.1250491529218465 1101 34-35 1.0415331460446424 1101 36-37 1.3751669518804732 1101 38-39 1.2254130349328065 1101 40-41 1.040449162299204 1101 42-43 1.545890893584918 1101 44-45 1.639230802264528 1101 46-47 1.334799621213044 1101 48-49 1.3521728749794093 1101 50-51 1.494263901383583 1101 52-53 1.2779556019799045 1101 54-55 1.2155074852279313 1101 56-57 1.5610868263842548 1101 58-59 1.7940626071734371 1101 60-61 1.708195556507377 1101 62-63 1.698881219563809 1101 64-65 1.1386446131369041 1101 66-67 0.8539608532302303 1101 68-69 0.49321230698350504 1101 70-71 0.6046435555837597 1101 72-73 0.5953023352624278 1101 74-75 0.9219483225287171 1101 76-77 0.8341416015326786 1101 78-79 0.9887165836281149 1101 80-81 0.8996429232762182 1101 82-83 1.4490514564677888 1101 84-85 1.6390754219634651 1101 86-87 1.2181372237649342 1101 88-89 1.2601022948680836 1101 90-91 1.425232784094856 1101 92-93 1.3942931555157294 1101 94-95 1.8248524802556076 1101 96-97 1.928945359097277 1101 98-99 2.0722377180257716 1101 100-101 1.7084057707958387 1102 1 0.3008330926967737 1102 2 0.32080848841365395 1102 3 0.4235259236419502 1102 4 0.2999423038225544 1102 5 0.33740825248564477 1102 6 0.49784018506715455 1102 7 0.22600762508741212 1102 8 0.40146133583981225 1102 9 0.5969778113916746 1102 10-11 0.33666539686085173 1102 12-13 0.6423461676943845 1102 14-15 0.5119512243815336 1102 16-17 0.5964861642342143 1102 18-19 0.7220850108096144 1102 20-21 0.37589969183448346 1102 22-23 0.5645724961164404 1102 24-25 0.5664114176762922 1102 26-27 0.1958601957132302 1102 28-29 0.5117000413497195 1102 30-31 0.6067394444087668 1102 32-33 0.5490629982718929 1102 34-35 0.6910237416559255 1102 36-37 0.44965101666625884 1102 38-39 0.8208610495618558 1102 40-41 0.9466665081717167 1102 42-43 1.5577443418607757 1102 44-45 1.5637344595268061 1102 46-47 0.7243633620698873 1102 48-49 1.0271010358989443 1102 50-51 0.7387780079666513 1102 52-53 1.0523482352087399 1102 54-55 1.4100281226365041 1102 56-57 1.5701646738241735 1102 58-59 1.2169335580616263 1102 60-61 0.9545056401019423 1102 62-63 1.5175855037853268 1102 64-65 1.7373253863866438 1102 66-67 1.7082581364068226 1102 68-69 1.3184147625738944 1102 70-71 1.5407067739745663 1102 72-73 1.3590497229322303 1102 74-75 1.5987704228421968 1102 76-77 1.4186831375828355 1102 78-79 1.7069114634609228 1102 80-81 1.3204240100055813 1102 82-83 1.543258614252533 1102 84-85 1.547937752162003 1102 86-87 1.4175886344232396 1102 88-89 1.7176519291418586 1102 90-91 1.5457265145023804 1102 92-93 2.035554911001622 1102 94-95 2.33824067252311 1102 96-97 2.3397995910722003 1102 98-99 1.680910654284915 1102 100-101 1.3492756767519474 1103 1 0.63991355246689 1103 2 0.46448664933319606 1103 3 0.2970891420327568 1103 4 -0.03339102951078132 1103 5 0.14775308007185117 1103 6 0.411633288515425 1103 7 -0.05560157031488444 1103 8 0.17157627836854772 1103 9 0.263644478058346 1103 10-11 0.5636768911137295 1103 12-13 0.6251047883840428 1103 14-15 0.7274684657608361 1103 16-17 0.7746470837744468 1103 18-19 0.4864528269015693 1103 20-21 0.5741755539034514 1103 22-23 0.7915839903693112 1103 24-25 0.6468711877912412 1103 26-27 0.7504578968626561 1103 28-29 0.6496310758324739 1103 30-31 0.6871992145237087 1103 32-33 1.20710897528339 1103 34-35 1.3404490290122482 1103 36-37 0.9209153844823561 1103 38-39 1.027757601285991 1103 40-41 1.1593101863326396 1103 42-43 1.09510066369986 1103 44-45 1.747642505503812 1103 46-47 1.1985012931043784 1103 48-49 1.6621585071633191 1103 50-51 1.8709619160126323 1103 52-53 2.3741873156685145 1103 54-55 2.226120076659484 1103 56-57 2.259819846237967 1103 58-59 2.248542753463923 1103 60-61 2.2159998929755034 1103 62-63 2.646895848612921 1103 64-65 2.70571619098434 1103 66-67 2.797338596176939 1103 68-69 2.1977251074014816 1103 70-71 2.282086084319392 1103 72-73 2.126291102242572 1103 74-75 2.414862376865184 1103 76-77 2.608338309996629 1103 78-79 2.8735781301275907 1103 80-81 2.866401021499833 1103 82-83 2.000155165976672 1103 84-85 1.9272480969895902 1103 86-87 2.3227610482163428 1103 88-89 2.335468021095881 1103 90-91 1.925036859329964 1103 92-93 2.380382497208519 1103 94-95 1.6974360748219617 1103 96-97 1.9001444186584067 1103 98-99 2.5918301945148023 1103 100-101 2.2429538376714895 1104 1 0.3897586897784713 1104 2 0.3299970079263588 1104 3 0.45308749016407646 1104 4 0.16170154396296255 1104 5 0.18313060089228372 1104 6 0.14939913613054046 1104 7 0.4118772651176954 1104 8 0.34760353420170986 1104 9 -0.022679425857965896 1104 10-11 0.29954997253709337 1104 12-13 -0.00787064006939886 1104 14-15 0.24816844511248348 1104 16-17 0.4808725638487843 1104 18-19 0.7102122046977044 1104 20-21 0.609432625966221 1104 22-23 0.6515364991448607 1104 24-25 0.5279194361221613 1104 26-27 0.3770323341948867 1104 28-29 0.6376206139975196 1104 30-31 0.629555880502096 1104 32-33 0.7627907152517253 1104 34-35 0.9041148009167159 1104 36-37 1.1974485250976699 1104 38-39 1.1265255825648168 1104 40-41 0.9480430654020822 1104 42-43 1.0715271211298258 1104 44-45 1.4753766923026248 1104 46-47 1.7286823103801652 1104 48-49 1.973243234260849 1104 50-51 1.4550695627880401 1104 52-53 1.7111857326276976 1104 54-55 1.7517928690058255 1104 56-57 1.804248230848053 1104 58-59 1.857583293074363 1104 60-61 1.9124518167142313 1104 62-63 2.1886743605545504 1104 64-65 2.035453268553347 1104 66-67 1.4836900312378525 1104 68-69 1.3502992694222655 1104 70-71 1.8812154118711852 1104 72-73 1.8663454762531764 1104 74-75 2.0429166132200614 1104 76-77 1.7200459292409 1104 78-79 1.0042650321855469 1104 80-81 1.1300117311150828 1104 82-83 1.5105137591351827 1104 84-85 1.2746395210380435 1104 86-87 1.0380201851149593 1104 88-89 1.0296314184391235 1104 90-91 0.6084459033109688 1104 92-93 0.7572150390214425 1104 94-95 0.7439120883122214 1104 96-97 0.7594774766802956 1104 98-99 1.081764119767744 1104 100-101 0.9152988029134193 1105 1 0.39050254368556736 1105 2 0.29414324960448823 1105 3 0.28969997947117676 1105 4 0.3867917362492008 1105 5 0.30995769205157586 1105 6 0.12120707305172829 1105 7 0.37554000175622093 1105 8 0.027969510323998747 1105 9 0.49824146527901547 1105 10-11 0.1600215759141861 1105 12-13 0.5521769665806531 1105 14-15 0.19635902048348441 1105 16-17 0.06898205914390587 1105 18-19 -0.14994326076887887 1105 20-21 0.3168934806543575 1105 22-23 0.41222010088548444 1105 24-25 0.4505586297845241 1105 26-27 0.1748528422471054 1105 28-29 0.17840240445387678 1105 30-31 -0.09598062412839425 1105 32-33 -0.27968334284325636 1105 34-35 0.30914325332866355 1105 36-37 0.2770408931565811 1105 38-39 -0.011794051459062871 1105 40-41 0.562894108657197 1105 42-43 0.46495145259113 1105 44-45 0.8176610676513079 1105 46-47 0.8076609989657229 1105 48-49 0.8492613842961774 1105 50-51 0.7308533988426404 1105 52-53 0.6542437160397512 1105 54-55 0.6043238319247166 1105 56-57 0.6657240369997268 1105 58-59 0.6254078923230679 1105 60-61 0.38325555086084506 1105 62-63 0.6160184301792739 1105 64-65 0.49130553916739217 1105 66-67 0.7614636764939213 1105 68-69 1.079480658197511 1105 70-71 0.3301870338446271 1105 72-73 0.3526957570580258 1105 74-75 0.4312827381131328 1105 76-77 0.8377236173429559 1105 78-79 0.8613521002853695 1105 80-81 0.6519368250266382 1105 82-83 0.3493020211205291 1105 84-85 0.6456924462435332 1105 86-87 0.7897775399704656 1105 88-89 0.867880386341831 1105 90-91 1.6643279280813026 1105 92-93 1.3250173226529398 1105 94-95 1.352626336833815 1105 96-97 1.6753104070927627 1105 98-99 1.8847730088219343 1105 100-101 1.858003455719608 1106 1 0.23278429500972564 1106 2 0.2102732652277055 1106 3 0.16962986553603443 1106 4 0.21585091393467337 1106 5 -0.0012491059517003578 1106 6 0.1901397408107499 1106 7 0.14050589742363684 1106 8 0.22097335896935277 1106 9 0.31625007709931197 1106 10-11 0.09724293018008723 1106 12-13 0.20856152627276714 1106 14-15 0.6026006849273315 1106 16-17 0.44891194520876354 1106 18-19 0.7634431660878036 1106 20-21 0.7501328206688385 1106 22-23 0.747528564094722 1106 24-25 0.6416000503538228 1106 26-27 0.7561697648550378 1106 28-29 0.42827856190539393 1106 30-31 0.6180574050603411 1106 32-33 0.6131452209642276 1106 34-35 1.0113114505622107 1106 36-37 0.7907588369892267 1106 38-39 1.051803860365041 1106 40-41 0.4786811756845921 1106 42-43 0.766174635916208 1106 44-45 1.2396212094033956 1106 46-47 1.2510363752565539 1106 48-49 0.8895578443045622 1106 50-51 0.6448495120918949 1106 52-53 0.17573516137755973 1106 54-55 0.3489604969401512 1106 56-57 0.8142588843876055 1106 58-59 0.8233082848086895 1106 60-61 1.0506906764181423 1106 62-63 1.0314244432113213 1106 64-65 0.8110860520660736 1106 66-67 0.44267390398315953 1106 68-69 0.5441569528284589 1106 70-71 0.6195975433138194 1106 72-73 0.2980526141246713 1106 74-75 0.28842559525599043 1106 76-77 0.6185985173162827 1106 78-79 0.6852098909343063 1106 80-81 0.9143877643247365 1106 82-83 0.7714336371705208 1106 84-85 0.8533287683103694 1106 86-87 1.2876436375965028 1106 88-89 1.7308914750130953 1106 90-91 1.3548549257568752 1106 92-93 1.4358087718012875 1106 94-95 1.3870736180135559 1106 96-97 1.4975106128619196 1106 98-99 1.1851769260570073 1106 100-101 1.4542576949453085 1107 1 -0.13744776687344284 1107 2 0.14507492662312416 1107 3 0.3145446999680708 1107 4 0.09486627041069084 1107 5 -0.08755783589873545 1107 6 0.25164185566043074 1107 7 0.19541577815373756 1107 8 0.09008131156623023 1107 9 0.18768245906783676 1107 10-11 0.6538103957899608 1107 12-13 0.6173943578850043 1107 14-15 0.4913900763840999 1107 16-17 0.2211559721873826 1107 18-19 0.483597111901922 1107 20-21 0.6647195676108808 1107 22-23 0.6234002251090871 1107 24-25 0.7362729154988088 1107 26-27 0.5692627801353041 1107 28-29 0.3212595473110227 1107 30-31 0.5717212392256457 1107 32-33 0.6861551664735117 1107 34-35 0.3774662346951203 1107 36-37 0.2925688434671443 1107 38-39 0.6683301721434205 1107 40-41 0.6156891397130906 1107 42-43 0.9587402724669047 1107 44-45 1.125114483800381 1107 46-47 0.9443069616986506 1107 48-49 0.8244166637992905 1107 50-51 0.8581825913892231 1107 52-53 1.023808933431063 1107 54-55 1.393143707701114 1107 56-57 1.122245776130164 1107 58-59 0.7900041656149952 1107 60-61 0.700025737196249 1107 62-63 1.2278196724153005 1107 64-65 1.46024963854628 1107 66-67 1.047177962208206 1107 68-69 1.4335179252782524 1107 70-71 1.264969642540283 1107 72-73 1.4582858191698271 1107 74-75 1.6300405020882884 1107 76-77 1.229027965169042 1107 78-79 1.1594887462481047 1107 80-81 1.1426200548403038 1107 82-83 0.9952647540397841 1107 84-85 1.2264377878584405 1107 86-87 0.7028210182313401 1107 88-89 0.8026629950374797 1107 90-91 0.5307875554104946 1107 92-93 0.6417875089883438 1107 94-95 0.6414462126102123 1107 96-97 0.23742220833499772 1107 98-99 0.2170711454678944 1107 100-101 0.16545687186867752 1108 1 0.141116439395514 1108 2 0.09246045312897166 1108 3 0.06793918212898475 1108 4 0.1586030897086843 1108 5 0.42916179431937707 1108 6 0.09968461169105325 1108 7 0.2958418939994658 1108 8 0.4496436400365482 1108 9 0.2066410030516579 1108 10-11 0.5173991578873256 1108 12-13 0.34403020939446805 1108 14-15 0.6219151377736694 1108 16-17 0.6088491687784554 1108 18-19 0.32302727223704153 1108 20-21 0.43694620346239077 1108 22-23 0.40265055011270334 1108 24-25 0.4622948712983188 1108 26-27 0.3399727324681052 1108 28-29 0.447379004193877 1108 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-2.930871693238199 2316 58-59 -2.930136349607487 2316 60-61 -2.7467380003729005 2316 62-63 -2.510631944867395 2316 64-65 -3.0886591623226103 2316 66-67 -2.782744313188058 2316 68-69 -2.5480352091615543 2316 70-71 -2.3642772094435607 2316 72-73 -2.4203925231578403 2316 74-75 -2.6104815085691477 2316 76-77 -2.6152343299129264 2316 78-79 -2.607625939968507 2316 80-81 -3.4296230975881628 2316 82-83 -3.0420532380180525 2316 84-85 -3.2598161577682774 2316 86-87 -3.982496159245496 2316 88-89 -3.8267705317588394 2316 90-91 -4.304989520922529 2316 92-93 -3.950124380500167 2316 94-95 -4.654127897290344 2316 96-97 -4.733405609606148 2316 98-99 -4.980668392257389 2316 100-101 -4.942084790555381 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 493.0 3 113.0 4 101.0 5 90.0 6 112.0 7 128.0 8 188.0 9 222.0 10 284.0 11 433.0 12 757.0 13 1044.0 14 1303.0 15 1795.0 16 2439.0 17 3482.0 18 2739.0 19 1447.0 20 1264.0 21 1221.0 22 1310.0 23 1554.0 24 1635.0 25 1935.0 26 1974.0 27 2227.0 28 2755.0 29 3236.0 30 4099.0 31 4924.0 32 6245.0 33 8127.0 34 11290.0 35 17598.0 36 21736.0 37 28864.0 38 24090.0 39 2898.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.6253612716763 21.121748554913296 15.695447976878613 14.557442196531792 2 14.410877406379278 27.1369129699101 34.09847713902053 24.3537324846901 3 27.276448950350087 25.421575769251607 34.68992131388355 12.612053966514752 4 10.821340398627086 19.788643343168545 43.40699704943699 25.983019208767388 5 8.354813066101368 43.53379745615726 35.59512911929081 12.516260358450568 6 21.783645101841703 40.534924898519655 16.927042555498005 20.75438744414064 7 19.262618415267966 41.12690985082536 22.06182586857938 17.548645865327288 8 23.326447400911835 25.6253048982468 21.22573612223634 29.822511578605027 9 19.894723528809497 24.56952884563265 28.669168087015702 26.86657953854215 10-11 24.23472230169031 25.71212609427559 26.914888170831965 23.138263433202138 12-13 30.24787465029226 27.634816284861607 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38-39 17.809010744274232 31.15719144069582 34.369940108947546 16.663857706082403 40-41 17.178551113706064 32.59888825018283 32.97960387517343 17.242956760937673 42-43 16.07575470306937 32.14609233695374 29.124270576586824 22.653882383390062 44-45 19.64742490174482 27.96164888142571 30.638463066283876 21.752463150545594 46-47 23.01296153070652 31.690897436283255 30.98759821963279 14.30854281337743 48-49 19.65314788009738 29.690974803116433 32.51309798587434 18.14277933091185 50-51 13.465832826729427 31.898590938912598 36.57840723731333 18.05716899704464 52-53 18.6337385149227 34.95375873889111 27.251797435287816 19.160705310898372 54-55 16.136061301021485 28.359547585941215 31.643702860995237 23.860688252042063 56-57 21.201421936470382 29.229162339753717 28.68374555644853 20.885670167327365 58-59 25.49418534659187 28.663352033322898 27.88806491223846 17.954397707846773 60-61 19.741075970513606 29.840377339180847 34.82621507972103 15.59233161058452 62-63 17.270034853692746 30.73866954003961 33.243139119808816 18.74815648645883 64-65 16.276921039642698 35.294400905293315 30.294822251917125 18.133855803146854 66-67 19.260452096439217 30.506877765403484 26.803118321644643 23.429551816512657 68-69 16.552834760621888 29.90683743960989 31.015757867645956 22.524569932122272 70-71 22.272590452110048 30.799169225212207 27.617542592258143 19.3106977304196 72-73 19.68298688242341 26.423880129791165 35.57518075045301 18.31795223733242 74-75 16.176346649329385 36.48805653880421 29.452912422644417 17.88268438922199 76-77 18.131246237206504 29.93527995183624 28.99277543648405 22.940698374473207 78-79 18.576421816999485 31.003453123918078 30.84298973696649 19.57713532211595 80-81 21.37967573165078 29.185355545253678 29.28409302997646 20.15087569311908 82-83 17.785972305839856 29.230885009030704 34.021372667068036 18.961770018061408 84-85 17.92194423039877 31.51668753612021 30.560405027932962 20.000963205548064 86-87 17.36197445537819 32.994283615740976 29.620141901186926 20.02360002769391 88-89 17.33442896315493 32.032724415990174 26.677382167119667 23.955464453735225 90-91 18.60108835674986 26.867001360445936 30.497465717966314 24.03444456483789 92-93 21.73710163950219 30.428931824886956 31.245672448776844 16.588294086834008 94-95 19.162750935410752 28.967180101563184 31.67964552221958 20.190423440806484 96-97 14.404154436667419 30.38097388424776 34.45954692556634 20.755324753518476 98-99 18.652916823485132 32.326383138878434 26.925404591644714 22.09529544599172 100-101 16.98525691985257 28.219026282190264 30.91921418419214 23.876502613765027 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2664.0 1 1601.0 2 482.5 3 396.0 4 312.0 5 296.5 6 468.0 7 858.0 8 1075.0 9 964.5 10 724.5 11 488.0 12 412.5 13 399.5 14 398.5 15 391.5 16 371.5 17 413.5 18 472.0 19 522.0 20 585.0 21 650.5 22 759.0 23 887.0 24 1045.0 25 1381.0 26 2145.5 27 3208.5 28 3479.0 29 3649.0 30 3946.0 31 4014.0 32 4088.5 33 3882.0 34 4645.5 35 5494.0 36 5730.0 37 5593.0 38 3923.5 39 2374.5 40 1998.5 41 1720.0 42 1546.5 43 1441.0 44 1346.5 45 1514.5 46 2953.5 47 10961.5 48 27018.0 49 25962.5 50 9619.5 51 2378.5 52 852.5 53 503.0 54 395.5 55 314.0 56 234.0 57 204.5 58 187.0 59 168.0 60 180.0 61 203.5 62 188.0 63 120.5 64 62.0 65 45.5 66 42.0 67 34.0 68 29.0 69 20.5 70 11.5 71 6.5 72 4.0 73 3.5 74 5.0 75 5.0 76 2.0 77 1.5 78 3.5 79 3.5 80 2.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04393540893032325 2 0.050555813015714435 3 0.03009274584268716 4 0.04995395809886068 5 0.06319476626964304 6 0.0668058957707655 7 0.06379662118649679 8 0.06861146052132673 9 0.06861146052132673 10-11 0.07131980764716857 12-13 0.0731253723977298 14-15 0.06861146052132673 16-17 0.05476879743369063 18-19 0.055069724892117505 20-21 0.058680854393239966 22-23 0.03520851263594398 24-25 0.02256955938201537 26-27 0.017152865130331683 28-29 0.023171414298869115 30-31 0.016250082755051066 32-33 0.009629678669659893 34-35 0.012338025795501738 36-37 0.006620404085391176 38-39 0.010833388503367378 40-41 0.01113431596179425 42-43 0.006921331543818047 44-45 0.002407419667414973 46-47 0.004513911876403074 48-49 9.027823752806149E-4 50-51 0.008425968835952405 52-53 0.008125041377525533 54-55 0.013240808170782352 56-57 0.02497697904943034 58-59 0.012638953253928609 60-61 0.02557883396628409 62-63 0.018055647505612296 64-65 0.011435243420221121 66-67 0.022870486840442242 68-69 0.027384398716845317 70-71 0.025879761424710955 72-73 0.024074196674149728 74-75 0.0222686319235885 76-77 0.031898310593248394 78-79 0.04303262655504264 80-81 0.03370387534380962 82-83 0.031898310593248394 84-85 0.024676051591003476 86-87 0.030995528217967777 88-89 0.023171414298869115 90-91 0.01865750242246604 92-93 0.03972242451234705 94-95 0.02979181838426029 96-97 0.03942149705392018 98-99 0.05446786997526376 100-101 0.06560218593705801 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 166153.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.073348058716963 #Duplication Level Percentage of deduplicated Percentage of total 1 79.30256468841034 16.71170547627789 2 9.74752955960473 4.108261662443651 3 3.532872565259611 2.233483596444241 4 1.5708002513280404 1.3240808170782352 5 0.8568001370880219 0.9027823752806148 6 0.665448106471697 0.8413931737615331 7 0.5226480836236934 0.7709761484896451 8 0.3341520534643286 0.5633362021751037 9 0.2541840406694465 0.48208578839984834 >10 2.35048837607814 10.372969491974263 >50 0.4055520648883304 5.92165052692398 >100 0.36556805849088936 15.905219887693873 >500 0.02856000456960073 4.591551160677207 >1k 0.05712000913920146 26.38170842536698 >5k 0.005712000913920146 8.888795267012933 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8600 5.175952284942191 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6169 3.7128429820707423 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4322 2.6012169506418785 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2972 1.7887128128893248 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2891 1.7399625646241719 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2703 1.626813840255668 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2645 1.5919062550781509 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2586 1.55639681498378 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2584 1.5551931051500725 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2445 1.4715352717074022 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2340 1.4083405054377591 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2174 1.308432589240038 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1884 1.1338946633524523 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1859 1.1188482904311088 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1785 1.0743110265839315 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1773 1.067088767581687 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1761 1.0598665085794419 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1584 0.9533381882963293 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1469 0.8841248728581488 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1424 0.8570414015997304 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1368 0.8233375262559208 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1265 0.7613464698199851 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 976 0.5874103988492534 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 935 0.5627343472582499 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 924 0.5561139431728588 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 840 0.5055581301571443 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 771 0.46403014089423605 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 769 0.46282643106052856 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 653 0.3930112607054943 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 628 0.3779648877841507 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 600 0.3611129501122459 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 533 0.3207886706830451 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 493 0.29671447400889545 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 481 0.2894922150066505 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 480 0.28889036008979674 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 480 0.28889036008979674 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 459 0.27625140683586813 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 444 0.267223583083062 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 434 0.26120503391452454 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 423 0.2545846298291334 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 421 0.2533809199954259 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 419 0.2521772101617184 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 419 0.2521772101617184 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 415 0.24976979049430342 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 398 0.23953825690778982 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 394 0.23713083724037484 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 391 0.2353252724898136 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 363 0.2184733348179088 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 352 0.21185293073251762 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 349 0.2100473659819564 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 340 0.20463067173027272 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 331 0.199213977478589 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 305 0.18356574964039168 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 304 0.18296389472353794 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 297 0.17875091030556176 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 292 0.175741635721293 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 291 0.1751397808044393 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 279 0.16791752180219435 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 276 0.16611195705163315 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 271 0.1631026824673644 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 266 0.1600934078830957 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 257 0.154676713631412 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 254 0.1528711488808508 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 241 0.14504703496175214 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 238 0.14324147021119088 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 235 0.14143590546062967 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 232 0.13963034071006844 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 229 0.1378247759595072 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 218 0.13120437187411602 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 213 0.12819509728984732 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 212 0.12759324237299358 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 211 0.12699138745613983 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 210 0.1263895325392861 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 207 0.12458396778872484 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 206 0.12398211287187111 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 204 0.12277840303816362 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 203 0.12217654812130987 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 201 0.12097283828760239 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 198 0.11916727353704117 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 191 0.11495428911906497 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 190 0.11435243420221122 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 190 0.11435243420221122 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 186 0.11194501453479623 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 184 0.11074130470108876 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 183 0.11013944978423501 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 180 0.10833388503367378 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 179 0.10773203011682003 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 178 0.1071301751999663 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 176 0.10592646536625881 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 175 0.10532461044940507 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 175 0.10532461044940507 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 174 0.10472275553255132 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 173 0.10412090061569758 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 172 0.10351904569884382 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 171 0.1029171907819901 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 169 0.10171348094828261 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 168 0.10111162603142887 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.009274584268716E-4 0.0 16-17 0.0 0.0 0.0 0.0012037098337074864 0.0 18-19 0.0 0.0 0.0 0.0012037098337074864 0.0 20-21 0.0 0.0 0.0 0.0021064922089881013 0.0 22-23 0.0 0.0 0.0 0.004513911876403074 0.0 24-25 0.0 0.0 0.0 0.007824113919098662 0.0 26-27 0.0 0.0 0.0 0.014745445462916708 0.0 28-29 0.0 0.0 0.0 0.03009274584268716 0.0 30-31 0.0 0.0 0.0 0.05567157980897125 0.0 32-33 0.0 0.0 0.0 0.08456061581795092 0.0 34-35 0.0 0.0 0.0 0.11344965182693059 0.0 36-37 0.0 0.0 0.0 0.15226929396399702 0.0 38-39 0.0 0.0 0.0 0.21365849548307886 0.0 40-41 0.0 0.0 0.0 0.25939946916396334 0.0 42-43 0.0 0.0 0.0 0.321089598141472 0.0 44-45 0.0 0.0 0.0 0.3581036755279772 0.0 46-47 0.0 0.0 0.0 0.4164836024627903 0.0 48-49 0.0 0.0 0.0 0.45560417205828363 0.0 50-51 0.0 0.0 0.0 0.49171546706950825 0.0 52-53 0.0 0.0 0.0 0.5654426943840918 0.0 54-55 0.0 0.0 0.0 0.7056748900110139 0.0 56-57 0.0 0.0 0.0 0.9428057272513888 0.0 58-59 0.0 0.0 0.0 1.1426215596468317 0.0 60-61 0.0 0.0 0.0 1.4101460701883204 0.0 62-63 0.0 0.0 0.0 1.5500773383568158 0.0 64-65 0.0 0.0 0.0 1.694823445860141 0.0 66-67 0.0 0.0 0.0 2.0448020800105926 0.0 68-69 0.0 0.0 0.0 2.5359156921632473 0.0 70-71 0.0 0.0 0.0 2.9496909475001956 0.0 72-73 0.0 0.0 0.0 3.3887441093450015 0.0 74-75 0.0 0.0 0.0 3.8608992916167626 0.0 76-77 0.0 0.0 0.0 4.178076832798686 0.0 78-79 0.0 0.0 0.0 4.588240958634511 0.0 80-81 0.0 6.018549168537432E-4 0.0 4.964400281668101 0.0 82-83 0.0 6.018549168537432E-4 0.0 5.334541055533153 0.0 84-85 0.0 6.018549168537432E-4 0.0 6.0218593705801275 0.0 86-87 0.0 6.018549168537432E-4 0.0 6.8614469795911 0.0 88-89 0.0 6.018549168537432E-4 0.0 7.859322431734606 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTTAG 80 1.8189894E-12 59.420193 1 GGCATTC 90 3.6379788E-12 52.80204 7 CGCAGAA 140 0.0 50.91626 9 CATTCCG 95 7.2759576E-12 50.022987 9 GAACTCG 50 2.8088305E-5 47.521835 9 TTAGGCA 105 2.0008883E-11 45.272526 4 TAGGCAT 105 2.0008883E-11 45.272526 5 GCATTCC 105 2.0008883E-11 45.258892 8 AGGCATT 105 2.0008883E-11 45.258892 6 CTTTAGG 110 3.274181E-11 43.214687 2 ATGGGGT 210 0.0 38.481647 5 TGGGGTG 210 0.0 36.20711 6 GGGAACT 55 0.002567505 34.561337 7 GGGTGAG 180 0.0 34.321327 8 GGTGAGA 185 0.0 33.393723 9 CATGGGG 385 0.0 33.337044 4 GGAACTC 75 3.058939E-4 31.681225 8 TGGGAAC 60 0.003932447 31.681225 6 GTAAGCG 30 0.0039497004 31.662151 94-95 GGGGTGA 200 0.0 30.889194 7 >>END_MODULE