##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936853_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 166635 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.924733699402886 33.0 31.0 34.0 30.0 34.0 2 31.970024304617876 33.0 31.0 34.0 30.0 34.0 3 31.95115071863654 33.0 31.0 34.0 30.0 34.0 4 35.481273441953974 37.0 35.0 37.0 33.0 37.0 5 35.51090107120353 37.0 35.0 37.0 33.0 37.0 6 35.74243106190176 37.0 35.0 37.0 35.0 37.0 7 35.737066042548086 37.0 35.0 37.0 35.0 37.0 8 35.735139676538545 37.0 35.0 37.0 35.0 37.0 9 37.24815915023854 39.0 37.0 39.0 34.0 39.0 10-11 37.15619467698863 39.0 37.0 39.0 33.5 39.0 12-13 37.130686830497794 39.0 37.0 39.0 34.0 39.0 14-15 38.09060221442074 40.0 38.0 41.0 33.0 41.0 16-17 38.08769466198578 40.0 38.0 41.0 33.0 41.0 18-19 38.13654994448945 40.0 38.0 41.0 34.0 41.0 20-21 38.006562246826896 40.0 37.5 41.0 33.5 41.0 22-23 37.89806763285024 40.0 37.5 41.0 33.5 41.0 24-25 37.90322861343655 40.0 37.0 41.0 33.0 41.0 26-27 37.744264410237946 40.0 37.0 41.0 33.5 41.0 28-29 37.70966183574879 40.0 37.0 41.0 33.0 41.0 30-31 37.57478020823956 39.5 36.5 41.0 33.0 41.0 32-33 37.19855972634801 39.0 35.0 41.0 33.0 41.0 34-35 36.87880097218472 39.0 35.0 41.0 32.0 41.0 36-37 36.52478170852462 38.5 35.0 40.5 31.0 41.0 38-39 36.247481021394066 38.0 35.0 40.0 30.5 41.0 40-41 36.236123863534075 39.0 35.0 40.0 30.5 41.0 42-43 35.84852221922165 38.0 35.0 40.0 30.0 41.0 44-45 35.381027395205095 38.0 35.0 40.0 28.5 41.0 46-47 34.666390614216695 38.0 35.0 40.0 24.0 41.0 48-49 34.046016743181205 38.0 34.0 40.0 20.5 41.0 50-51 33.16360608515618 37.5 33.5 39.5 14.5 40.5 52-53 33.441095808203556 38.0 33.5 39.5 13.5 40.5 54-55 33.85276502535481 38.0 34.0 40.0 10.0 41.0 56-57 33.61036096858403 38.0 34.0 40.0 7.5 41.0 58-59 33.40402976565547 38.0 34.0 40.0 4.0 41.0 60-61 32.82449065322412 38.0 33.0 40.0 2.0 41.0 62-63 32.61267440813754 37.0 32.5 40.0 2.0 41.0 64-65 32.27860293455757 37.0 32.5 39.5 2.0 41.0 66-67 31.61488582830738 36.0 31.0 39.0 2.0 40.5 68-69 31.095199087826686 36.0 31.0 38.5 2.0 39.5 70-71 30.391376361508684 35.0 30.0 37.5 2.0 39.0 72-73 29.910877066642662 35.0 29.5 37.0 2.0 39.0 74-75 29.533831427971315 35.0 29.0 36.5 2.0 38.0 76-77 29.116677168662044 34.0 29.0 36.0 2.0 37.0 78-79 28.61145317610346 34.0 29.0 35.5 2.0 37.0 80-81 28.031590002100398 34.0 27.0 35.0 2.0 36.5 82-83 27.54033666396615 34.0 26.5 35.0 2.0 36.0 84-85 27.12820835958832 34.0 26.0 35.0 2.0 36.0 86-87 27.06871305548054 34.0 26.0 35.0 2.0 35.0 88-89 26.498421700123025 34.0 24.5 35.0 2.0 35.0 90-91 26.005010952080895 33.0 23.5 35.0 2.0 35.0 92-93 25.627062141806945 33.0 20.0 35.0 2.0 35.0 94-95 25.12837339094428 33.0 18.0 35.0 2.0 35.0 96-97 24.511564197197465 32.0 4.5 35.0 2.0 35.0 98-99 24.05890419179644 32.0 2.0 35.0 2.0 35.0 100-101 21.64580670327362 28.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05043647947230667 1101 2 0.0890139122084328 1101 3 0.24737958347324351 1101 4 0.3147363328902486 1101 5 0.18935287371112253 1101 6 -0.264671263549495 1101 7 0.006510069191726586 1101 8 -0.12920472063245114 1101 9 -0.2790555991099808 1101 10-11 0.171068797583807 1101 12-13 0.1709969205456332 1101 14-15 0.3214477360687411 1101 16-17 0.48613237873669135 1101 18-19 -0.10724674829744885 1101 20-21 -0.25853890560077986 1101 22-23 -0.6405134645408808 1101 24-25 -0.41654252437010797 1101 26-27 -0.588713836392806 1101 28-29 -0.5635555705203288 1101 30-31 -0.23018757192808437 1101 32-33 0.32758650248356247 1101 34-35 0.1919917437226175 1101 36-37 -0.09579010478584138 1101 38-39 0.30740064146053925 1101 40-41 -0.1996944748879983 1101 42-43 -0.020821221734600215 1101 44-45 0.33522625226749625 1101 46-47 0.5839288572167689 1101 48-49 0.9088998184050823 1101 50-51 1.0370803889312867 1101 52-53 1.3726081738786178 1101 54-55 1.518931763679781 1101 56-57 1.5352245842129548 1101 58-59 1.3567232677752585 1101 60-61 1.5673653071682168 1101 62-63 1.7731442591705786 1101 64-65 1.6987624171339064 1101 66-67 1.660900083564183 1101 68-69 1.665151235283556 1101 70-71 1.7487798820802247 1101 72-73 1.2652694225208556 1101 74-75 1.2389917859619715 1101 76-77 1.6955188054202175 1101 78-79 1.6322236591968284 1101 80-81 2.0035606882683545 1101 82-83 1.5909263081217553 1101 84-85 1.3628953630378575 1101 86-87 1.5112831122980275 1101 88-89 1.1200228792455391 1101 90-91 1.1883633084851546 1101 92-93 1.116086581932386 1101 94-95 1.2772553587668796 1101 96-97 1.1143363773360377 1101 98-99 1.1520079399542276 1101 100-101 1.157867610293728 1102 1 0.32433353829583567 1102 2 0.7221021475025502 1102 3 0.619438407002658 1102 4 0.35186868583142683 1102 5 0.5496469913581876 1102 6 0.38018167762697885 1102 7 0.2870247750740802 1102 8 0.3612364558381387 1102 9 0.744473812654725 1102 10-11 0.44239232699558073 1102 12-13 0.6654822146632782 1102 14-15 0.5914845007746266 1102 16-17 0.9197720846190478 1102 18-19 0.6502900164084409 1102 20-21 0.4626008002815851 1102 22-23 0.6971703589885294 1102 24-25 0.37757512268871807 1102 26-27 0.6287493989013058 1102 28-29 0.5925106059502596 1102 30-31 0.9299227221895592 1102 32-33 0.9367776789541509 1102 34-35 0.8316976260755524 1102 36-37 0.9202025422729818 1102 38-39 1.2737609355781885 1102 40-41 1.051959936876706 1102 42-43 1.2565684841477562 1102 44-45 0.821071840502789 1102 46-47 0.5876053278050009 1102 48-49 0.3737895242874387 1102 50-51 0.8240289183430463 1102 52-53 0.43584346799626417 1102 54-55 0.4799611754444797 1102 56-57 0.5054451724482476 1102 58-59 0.5846644442458455 1102 60-61 0.6186520718740951 1102 62-63 1.0010854356411656 1102 64-65 1.0079535936044977 1102 66-67 1.2103486129759453 1102 68-69 1.3320629999894358 1102 70-71 1.1403239997272827 1102 72-73 0.7847547166385063 1102 74-75 1.1946903153737338 1102 76-77 1.5309967465966885 1102 78-79 1.599319247432124 1102 80-81 1.2403253941507089 1102 82-83 1.386330719886459 1102 84-85 1.2088512453908002 1102 86-87 0.9408787005333217 1102 88-89 0.8869346439514239 1102 90-91 0.9220030143675082 1102 92-93 0.8603880525206193 1102 94-95 1.4186156528845295 1102 96-97 1.0692996126301537 1102 98-99 1.5086255870130536 1102 100-101 1.5529043749996099 1103 1 0.11824717311392519 1103 2 0.22492431683270908 1103 3 0.44810212682584805 1103 4 0.2165065641041224 1103 5 0.26579796041632875 1103 6 0.13019867864703372 1103 7 0.07294793046339976 1103 8 0.37765944121726136 1103 9 0.3296149211212338 1103 10-11 0.35755723689017316 1103 12-13 0.5948769783490988 1103 14-15 0.7906854528317453 1103 16-17 0.5698570897193491 1103 18-19 0.4691441476563156 1103 20-21 0.5759806030697447 1103 22-23 0.4506895701411935 1103 24-25 0.48981585713278264 1103 26-27 0.8977565393297269 1103 28-29 0.8982399497108844 1103 30-31 0.8645197980141148 1103 32-33 0.7537786990153563 1103 34-35 0.6707489691561364 1103 36-37 1.0847554154453718 1103 38-39 0.9395718842351144 1103 40-41 0.6472166522796314 1103 42-43 0.528907824508174 1103 44-45 0.8081850672963995 1103 46-47 1.2816889728237015 1103 48-49 1.1305219282316799 1103 50-51 1.4643383369081562 1103 52-53 1.5435619311040512 1103 54-55 1.3026745382462508 1103 56-57 1.438512156467283 1103 58-59 1.035191475867741 1103 60-61 1.5406853938734173 1103 62-63 1.262017091540521 1103 64-65 1.1180181974807226 1103 66-67 0.9234904014832601 1103 68-69 1.2078535474222853 1103 70-71 1.1434691884386048 1103 72-73 0.7621263589370422 1103 74-75 1.264190485383935 1103 76-77 1.2298576782525892 1103 78-79 1.3003053065956713 1103 80-81 1.2866531738174878 1103 82-83 1.24777240638765 1103 84-85 1.1877869815349698 1103 86-87 1.6135410602749083 1103 88-89 1.59596218560392 1103 90-91 1.4830706784273495 1103 92-93 1.5674774778861433 1103 94-95 2.063328335645494 1103 96-97 2.1056477935210083 1103 98-99 1.6254905989137676 1103 100-101 1.5258047490220505 1104 1 0.6360982441781893 1104 2 0.5397492063260749 1104 3 0.5782619364144246 1104 4 0.6989275093608427 1104 5 0.6437646384170108 1104 6 0.47429932468580205 1104 7 0.6046718338976049 1104 8 0.5024129264263735 1104 9 0.17976793030178584 1104 10-11 0.6776864446426387 1104 12-13 0.5448939793691636 1104 14-15 0.70619038312757 1104 16-17 0.6374191434425782 1104 18-19 0.6061723693496148 1104 20-21 0.9567184473403998 1104 22-23 0.9677585942826497 1104 24-25 0.7246339462181339 1104 26-27 0.4522788106660087 1104 28-29 0.6513341353620277 1104 30-31 0.23580507513074167 1104 32-33 0.7338365024835554 1104 34-35 0.8522858613696798 1104 36-37 1.0849084246259224 1104 38-39 0.9090550532252522 1104 40-41 0.5872540545237612 1104 42-43 0.3124508370889245 1104 44-45 -0.22010463008545145 1104 46-47 0.24642885721676322 1104 48-49 0.7355542301697895 1104 50-51 0.7799112712842202 1104 52-53 0.5211375856433236 1104 54-55 0.8681964695621289 1104 56-57 1.2113275253894216 1104 58-59 0.9964291501282005 1104 60-61 1.2539461895211588 1104 62-63 1.086379553288218 1104 64-65 0.9403065347809658 1104 66-67 0.4368192012112466 1104 68-69 0.5379453529306133 1104 70-71 0.7550298820802261 1104 72-73 0.7465194225208585 1104 74-75 0.9829256094913816 1104 76-77 0.9780555701260987 1104 78-79 0.9934368944909444 1104 80-81 0.8579724529742414 1104 82-83 0.27750719047469374 1104 84-85 0.8117924218613872 1104 86-87 0.3879375240627354 1104 88-89 -0.036594767813280527 1104 90-91 0.46317948495574157 1104 92-93 0.833917464285328 1104 94-95 1.121556829355118 1104 96-97 1.5281231420419203 1104 98-99 1.590978528189524 1104 100-101 1.732316139705489 1105 1 0.541469088167954 1105 2 0.3869176389165041 1105 3 0.45126157105088893 1105 4 0.19816024593372816 1105 5 0.3901292712266553 1105 6 -0.012730269760673707 1105 7 0.13861410645880312 1105 8 0.28345055887065485 1105 9 0.1998263884676632 1105 10-11 0.38977997770803796 1105 12-13 0.4583028211667539 1105 14-15 0.42138950625508187 1105 16-17 0.45459122966837384 1105 18-19 0.5820583759261595 1105 20-21 0.5443143552687886 1105 22-23 0.31160221868893245 1105 24-25 0.44432704084728414 1105 26-27 0.15288166050159901 1105 28-29 0.38335824935544593 1105 30-31 0.13765016099117133 1105 32-33 0.045327185713375684 1105 34-35 -0.09356726248856262 1105 36-37 0.13509110639428457 1105 38-39 -0.26385324052703396 1105 40-41 0.26352369281386956 1105 42-43 0.41281620062564883 1105 44-45 -0.6280695551859239 1105 46-47 0.09549718019813014 1105 48-49 -0.3078509579924287 1105 50-51 -0.31771386572710014 1105 52-53 -0.6350005217735557 1105 54-55 -0.6690340748295398 1105 56-57 -0.5893483971535147 1105 58-59 0.14476674603613304 1105 60-61 -0.11138081084421003 1105 62-63 -0.09781847374868136 1105 64-65 -0.16362112323876232 1105 66-67 -0.045058767367489594 1105 68-69 -0.006665534902783321 1105 70-71 0.02148950940941674 1105 72-73 -0.08950542219963964 1105 74-75 -0.10699035689517444 1105 76-77 0.09802268740779496 1105 78-79 0.4110474169608018 1105 80-81 0.24936814168450638 1105 82-83 0.23360876153790144 1105 84-85 0.028306853721090874 1105 86-87 0.14204786385082357 1105 88-89 0.3342309537796986 1105 90-91 0.22825073084540293 1105 92-93 0.4412612713733779 1105 94-95 0.9241691786426571 1105 96-97 0.9241772158453543 1105 98-99 0.8648184989604424 1105 100-101 0.8452317407285079 1106 1 0.2546154735551447 1106 2 0.28820030274097874 1106 3 0.4806636071418886 1106 4 0.3803058595174704 1106 5 0.2599150038886435 1106 6 0.15581690213097943 1106 7 0.008987879842614177 1106 8 0.1485319657580817 1106 9 0.37124025887818846 1106 10-11 0.5492492709565937 1106 12-13 0.3887853820841016 1106 14-15 0.7108893041160869 1106 16-17 0.4020902189733775 1106 18-19 0.24365931679131592 1106 20-21 0.34994852043472946 1106 22-23 0.4370937839798259 1106 24-25 0.49114978332220005 1106 26-27 0.8377469624237577 1106 28-29 0.7061374768169486 1106 30-31 0.5745276647583069 1106 32-33 0.6057299936078238 1106 34-35 0.7495361224208423 1106 36-37 0.9725345993561731 1106 38-39 -0.12601282007792491 1106 40-41 0.4287263831001624 1106 42-43 0.2593008196855138 1106 44-45 0.4339503646935299 1106 46-47 0.6801566678676565 1106 48-49 1.1658709722512413 1106 50-51 0.5039627853809918 1106 52-53 0.4582590614525799 1106 54-55 0.5729998110170627 1106 56-57 1.1416964777040732 1106 58-59 1.1429658713255506 1106 60-61 1.2330272894167393 1106 62-63 0.9416353834309348 1106 64-65 1.0916988076664467 1106 66-67 0.8950162078245363 1106 68-69 1.2570891051060364 1106 70-71 1.430177811074305 1106 72-73 1.528176227254587 1106 74-75 1.5654525847785408 1106 76-77 1.658407119029686 1106 78-79 2.0701859373033358 1106 80-81 1.4217559545405472 1106 82-83 1.477002491553705 1106 84-85 1.3935536470615304 1106 86-87 1.5466566330080873 1106 88-89 1.1548601573520472 1106 90-91 1.6427826871833808 1106 92-93 1.7976139487962897 1106 94-95 1.8061200037372984 1106 96-97 2.392202501596394 1106 98-99 2.177821549421683 1106 100-101 1.5483446812996462 1107 1 0.1924007651865942 1107 2 0.44008534077986283 1107 3 0.03237958347324721 1107 4 0.08312919003310526 1107 5 0.29149573085398117 1107 6 0.1892573078790747 1107 7 -0.08473993080827569 1107 8 -0.13170472063244887 1107 9 0.15237297231859515 1107 10-11 -0.13928834527333578 1107 12-13 0.3072469205456301 1107 14-15 0.08350130749731477 1107 16-17 0.1365788073081191 1107 18-19 0.439449680273988 1107 20-21 0.37797895154208305 1107 22-23 0.2643972497448317 1107 24-25 0.22060033277274727 1107 26-27 -0.12898169353567113 1107 28-29 0.020998000908242886 1107 30-31 0.0374017137861955 1107 32-33 -0.001877783230725072 1107 34-35 -0.05443682770595615 1107 36-37 -0.352486533357272 1107 38-39 -0.01876007282517378 1107 40-41 -0.25299804631656997 1107 42-43 -0.8309105074488841 1107 44-45 -1.1753987477325083 1107 46-47 -0.7821425713546617 1107 48-49 -0.7451180387377647 1107 50-51 -1.1676517539258597 1107 52-53 -0.8438203975499547 1107 54-55 -0.5464253791773643 1107 56-57 -0.5753111300727625 1107 58-59 -0.09399101793902531 1107 60-61 -0.3364739785460671 1107 62-63 -0.08899859797227805 1107 64-65 -0.11355901143752334 1107 66-67 -0.17276063072153036 1107 68-69 -0.060205907573585904 1107 70-71 -0.1506844036340631 1107 72-73 0.04651942252085561 1107 74-75 -0.24984749975231324 1107 76-77 -0.34421333743692273 1107 78-79 -0.46000848366031377 1107 80-81 -0.5557250260173596 1107 82-83 -0.24291297759253538 1107 84-85 -0.4687117798192837 1107 86-87 -0.05273474484483032 1107 88-89 0.13895145067411008 1107 90-91 0.030238308485156296 1107 92-93 0.6777830105038127 1107 94-95 0.7989517873383107 1107 96-97 1.1444256630503205 1107 98-99 0.4402222256685171 1107 100-101 0.15492118172230107 1108 1 -0.11930222109266708 1108 2 -0.19707365841303925 1108 3 -0.13665996454935225 1108 4 -0.047234006092800485 1108 5 -0.05626859521542826 1108 6 0.0548908833431625 1108 7 0.026672498570256664 1108 8 0.11055516637319585 1108 9 0.06953197151148771 1108 10-11 0.1265066506911623 1108 12-13 0.1532356211106034 1108 14-15 0.10133827279190655 1108 16-17 0.23851585331296832 1108 18-19 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-0.9641256794797286 2316 64-65 -1.1357851288783642 2316 66-67 -1.4340577385217053 2316 68-69 -0.8501095009127617 2316 70-71 -0.3879149031958491 2316 72-73 -1.3320081848411078 2316 74-75 -2.120574931829438 2316 76-77 -2.697675212984688 2316 78-79 -3.0292985800669747 2316 80-81 -3.1637706000751997 2316 82-83 -2.360511575313833 2316 84-85 -2.622419054140053 2316 86-87 -3.0705612128553454 2316 88-89 -3.656280801736056 2316 90-91 -3.411234084152884 2316 92-93 -3.754714798435714 2316 94-95 -3.042012279269926 2316 96-97 -3.166932794443106 2316 98-99 -4.100829483358655 2316 100-101 -4.023133156577437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 400.0 3 109.0 4 98.0 5 98.0 6 112.0 7 116.0 8 171.0 9 218.0 10 309.0 11 467.0 12 746.0 13 1055.0 14 1267.0 15 1770.0 16 2441.0 17 3448.0 18 2833.0 19 1350.0 20 1329.0 21 1237.0 22 1321.0 23 1532.0 24 1762.0 25 1950.0 26 2001.0 27 2386.0 28 2835.0 29 3442.0 30 4260.0 31 5193.0 32 6562.0 33 8478.0 34 11473.0 35 17717.0 36 21900.0 37 28562.0 38 22968.0 39 2719.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.42249327698809 21.341360929696503 15.722843834037647 14.513301959277754 2 14.382517465728759 27.30235517249658 33.91292343888795 24.402203922886706 3 27.12584625726221 25.241273347097522 35.07346233254909 12.55941806309118 4 10.730664873820443 19.746320263164215 43.573966912382645 25.949047950632696 5 8.357352164096651 43.61759619541721 35.54847000048038 12.476581640005763 6 21.910422670509124 40.3866474543708 16.802953890489913 20.899975984630164 7 19.182958085485684 41.096391978439506 22.059291360796156 17.66135857527866 8 23.36147269321293 25.934242759018204 21.00965464239397 29.694629905374896 9 19.846805854033352 24.282952949227425 28.857767159305105 27.01247403743412 10-11 24.33437480303617 25.541219089809502 27.053601174129533 23.070804933024792 12-13 30.169569876657356 27.635777472931554 25.846123366662965 16.348529283748128 14-15 23.206257165667225 29.21311219559045 33.29851794493166 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17.179164041167823 32.538482311639214 33.01527290185135 17.267080745341616 42-43 16.282893749812462 32.14360728538422 29.06112161310649 22.51237735169682 44-45 19.814564767305786 27.822486272391757 30.575809403786717 21.78713955651574 46-47 23.099588921895158 31.67221772136706 30.84015963032976 14.388033726408016 48-49 19.71104509856873 29.77675758394095 32.32274132085096 18.18945599663936 50-51 13.61435009871994 31.900343869461633 36.41325547760648 18.072050554211952 52-53 18.732915452682366 34.952245783436204 27.155541019416745 19.15929774446468 54-55 16.2572278184366 28.29674039266521 31.507437113853705 23.938594675044484 56-57 21.320078735432144 29.23983100687735 28.585702797748358 20.854387459942146 58-59 25.517013743023465 28.732521154654023 27.87763307927744 17.87283202304507 60-61 19.781011165489904 29.77984090452171 34.65832880732403 15.780819122664354 62-63 17.332205098660523 30.631871429257284 33.21390849296654 18.822014979115657 64-65 16.41031488360649 35.159902300264655 30.257812078039763 18.171970738089094 66-67 19.256496205357813 30.40785276368187 26.73624761053077 23.59940342042955 68-69 16.699927676060778 29.76175113962722 31.0392739996939 22.49904718461811 70-71 22.409037567492128 30.74669780033435 27.64033410667283 19.203930525500695 72-73 19.714294288886887 26.43588127607215 35.58386603043126 18.265958404609705 74-75 16.11734693877551 36.617947178871546 29.29891956782713 17.96578631452581 76-77 18.154422446602876 29.87537669135921 28.98182276596511 22.988378096072807 78-79 18.67009207793611 31.04899218478013 30.81459561339272 19.46632012389104 80-81 21.421731233958244 29.058133077165543 29.303994116146075 20.216141572730137 82-83 18.008896839880894 29.03539525501873 34.03581548362309 18.919892421477282 84-85 17.89717564651615 31.511453772257052 30.52978761077882 20.06158297044798 86-87 17.368365769848296 32.868568341761325 29.6544273216992 20.108638566691177 88-89 17.35434016055218 32.01020331607772 26.609047940580687 24.026408582789408 90-91 18.63037873626522 26.673609714664735 30.511394502186462 24.184617046883584 92-93 21.850500054027446 30.12390294269489 31.438870946440794 16.586726056836874 94-95 19.15989940640174 29.014890973585178 31.709190869750497 20.116018750262587 96-97 14.378539636424753 30.382573358824217 34.346365898238545 20.892521106512483 98-99 18.42342207149074 32.17653591044149 27.150274618085778 22.249767399981994 100-101 17.055343265123568 28.16001729008012 30.871798258384636 23.91284118641168 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2596.0 1 1549.0 2 459.5 3 365.0 4 300.0 5 320.0 6 482.0 7 866.5 8 1093.0 9 953.5 10 676.0 11 456.5 12 402.5 13 392.5 14 352.0 15 345.0 16 362.0 17 388.0 18 432.5 19 494.0 20 563.5 21 659.0 22 744.5 23 864.0 24 1062.5 25 1399.0 26 2203.5 27 3256.0 28 3465.5 29 3560.5 30 3926.0 31 4171.0 32 4185.5 33 3874.5 34 4583.5 35 5483.0 36 5870.0 37 5667.5 38 3964.5 39 2418.5 40 2007.0 41 1719.0 42 1516.0 43 1428.0 44 1367.5 45 1508.5 46 2969.0 47 11118.5 48 27131.0 49 25944.5 50 9626.5 51 2431.0 52 890.0 53 530.0 54 417.0 55 318.0 56 252.5 57 221.0 58 206.0 59 193.5 60 189.0 61 192.5 62 161.5 63 107.0 64 62.0 65 44.0 66 38.0 67 37.5 68 28.0 69 17.5 70 13.5 71 11.5 72 6.5 73 5.0 74 3.5 75 3.0 76 3.0 77 2.0 78 1.5 79 1.0 80 1.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025804902931556992 2 0.013802622498274674 3 0.011402166411618208 4 0.02820535901821346 5 0.059411288144747505 6 0.04500855162480871 7 0.02040387673657995 8 0.049809463798121636 9 0.029405587061541692 10-11 0.026104959942389053 12-13 0.03990758244066373 14-15 0.02520478890989288 16-17 0.017403306628259372 18-19 0.020703933747412008 20-21 0.01410267950910673 22-23 0.01110210940078615 24-25 0.0027005130974885228 26-27 0.0 28-29 0.003000570108320581 30-31 0.0 32-33 6.001140216641161E-4 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0012002280433282323 52-53 3.000570108320581E-4 54-55 0.0027005130974885228 56-57 6.001140216641161E-4 58-59 0.003000570108320581 60-61 0.003300627119152639 62-63 0.0018003420649923487 64-65 0.0012002280433282323 66-67 0.01110210940078615 68-69 0.01410267950910673 70-71 0.024904731899060818 72-73 0.018003420649923486 74-75 0.021003990758244065 76-77 0.03180604314819816 78-79 0.0222042188015723 80-81 0.046508836678969004 82-83 0.03540672727818286 84-85 0.018303477660755543 86-87 0.016503135595763194 88-89 0.013502565487442613 90-91 0.024904731899060818 92-93 0.03180604314819816 94-95 0.01440273651993879 96-97 0.023704503855732587 98-99 0.024004560866564648 100-101 0.03930746841899961 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 166635.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.030395775197288 #Duplication Level Percentage of deduplicated Percentage of total 1 79.18616596278963 16.653164101179225 2 9.861887912338773 4.147988117742371 3 3.273028193128638 2.0649923485462236 4 1.7549366510672297 1.4762804932937257 5 0.9930373245063349 1.044198397695562 6 0.6334893277023171 0.7993518768566028 7 0.425179773998402 0.6259189245956731 8 0.3538408857436366 0.5953131094908032 9 0.30818399726058665 0.5833108290575209 >10 2.35703686793745 10.475590362168811 >50 0.39093710763611456 5.789900081015393 >100 0.3652551078643991 15.568157950010503 >500 0.03138911083209679 4.633480361268641 >1k 0.05992466613400297 26.66066552645003 >5k 0.0057071110603812356 8.881687520628919 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8542 5.12617397305488 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6258 3.7555135475740387 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4244 2.546883907942509 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2923 1.7541332853242115 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2783 1.6701173222912356 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2742 1.6455126474030066 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2616 1.5698982806733277 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2600 1.560296456326702 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2540 1.5242896150268552 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2397 1.4384733099288867 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2330 1.3982656704773908 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2241 1.3448555225492844 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1849 1.1096108260569508 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1819 1.0916074054070273 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1800 1.080205238995409 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1760 1.0562006781288444 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1701 1.0207939508506616 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1517 0.9103729708644642 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1479 0.8875686380412279 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1455 0.8731659015212891 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1372 0.8233564377231674 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1249 0.7495424130584811 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1009 0.6055150478590932 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 968 0.5809103729708645 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 903 0.5419029615626969 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 834 0.5004950940678728 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 814 0.48849281363459063 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 779 0.4674888228763465 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 661 0.39667536831998085 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 626 0.37567137756173674 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 606 0.3636690971284544 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 520 0.3120592912653404 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 505 0.30305758094037866 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 505 0.30305758094037866 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 494 0.2964563267020734 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 485 0.2910553005070963 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 483 0.2898550724637681 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 477 0.2862543883337834 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 471 0.2826537042037987 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 465 0.27905302007381405 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 424 0.2544483451855853 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 421 0.25264800312059293 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 393 0.23584481051399767 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 389 0.23344435442734118 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 381 0.22864344225402827 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 380 0.22804332823236415 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 369 0.22144207399405888 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 362 0.21724127584241007 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 356 0.2136405917124254 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 345 0.2070393374741201 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 330 0.19803762714915835 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 320 0.19203648693251718 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 317 0.19023614486752483 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 315 0.1890359168241966 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 309 0.18543523269421192 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 305 0.18303477660755543 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 302 0.18123443454256308 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 300 0.18003420649923485 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 293 0.17583340834758604 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 291 0.1746331803042578 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 288 0.17283283823926546 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 265 0.15903021574099077 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 260 0.1560296456326702 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 259 0.1554295316110061 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 247 0.1482281633510367 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 242 0.1452275932427161 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 235 0.1410267950910673 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 235 0.1410267950910673 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 221 0.13262519878776968 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 221 0.13262519878776968 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 220 0.13202508476610555 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 218 0.13082485672277733 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 215 0.129024514657785 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 213 0.12782428661445674 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 212 0.12722417259279264 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 206 0.12362348846280792 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 206 0.12362348846280792 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 205 0.12302337444114382 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 198 0.11882257628949501 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 197 0.11822246226783088 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 197 0.11822246226783088 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 196 0.11762234824616677 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 194 0.11642212020283854 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 193 0.11582200618117443 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 192 0.11522189215951031 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 191 0.11462177813784619 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 186 0.1116212080295256 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 184 0.11042097998619739 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 181 0.10862063792120503 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 180 0.10802052389954091 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 179 0.1074204098778768 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 175 0.10501995379122034 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 175 0.10501995379122034 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 174 0.10441983976955621 No Hit GAGTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAG 172 0.10321961172622797 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 171 0.10261949770456387 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 168 0.10081915563957151 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.001140216641161E-4 0.0 14-15 0.0 0.0 0.0 6.001140216641161E-4 0.0 16-17 0.0 0.0 0.0 0.0015002850541602905 0.0 18-19 0.0 0.0 0.0 0.0018003420649923484 0.0 20-21 0.0 0.0 0.0 0.0024004560866564646 0.0 22-23 0.0 0.0 0.0 0.003600684129984697 0.0 24-25 0.0 0.0 0.0 0.007201368259969395 0.0 26-27 0.0 0.0 0.0 0.013502565487442615 0.0 28-29 0.0 0.0 0.0 0.030005701083205806 0.0 30-31 0.0 0.0 0.0 0.051309748852281935 0.0 32-33 0.0 0.0 0.0 0.08101539292465568 0.0 34-35 0.0 0.0 0.0 0.1110210940078615 0.0 36-37 0.0 0.0 0.0 0.13772616797191467 0.0 38-39 0.0 0.0 0.0 0.1839349476400516 0.0 40-41 0.0 0.0 0.0 0.23224412638401298 0.0 42-43 0.0 0.0 0.0 0.2793530770846461 0.0 44-45 0.0 0.0 0.0 0.3216611156119663 0.0 46-47 0.0 0.0 0.0 0.3831728028325382 0.0 48-49 0.0 0.0 0.0 0.42218021424070573 0.0 50-51 0.0 0.0 0.0 0.45218591532391156 0.0 52-53 0.0 0.0 0.0 0.5235994839019413 0.0 54-55 0.0 0.0 0.0 0.6592252527980316 0.0 56-57 0.0 0.0 0.0 0.8755663576079455 0.0 58-59 0.0 0.0 0.0 1.069103129594623 0.0 60-61 0.0 0.0 0.0 1.3151498784769107 0.0 62-63 0.0 0.0 0.0 1.4441743931346955 0.0 64-65 0.0 0.0 0.0 1.5966033546373812 0.0 66-67 0.0 0.0 0.0 1.9485702283433852 0.0 68-69 0.0 0.0 0.0 2.4499654934437545 0.0 70-71 0.0 0.0 0.0 2.8961502685510245 0.0 72-73 0.0 0.0 0.0 3.2976265490443186 0.0 74-75 0.0 0.0 0.0 3.7792180514297717 0.0 76-77 0.0 0.0 0.0 4.125783898940799 0.0 78-79 0.0 0.0 0.0 4.511057100849161 0.0 80-81 0.0 0.0 0.0 4.846520838959402 0.0 82-83 0.0 0.0 0.0 5.226393014672787 0.0 84-85 0.0 0.0 0.0 5.908422600294056 0.0 86-87 0.0 0.0 0.0 6.757283883937948 0.0 88-89 0.0 0.0 0.0 7.729468599033817 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTTTAG 115 5.4569682E-11 41.341843 1 CTTTAGG 120 8.54925E-11 39.607365 2 AGGCATT 125 1.3278623E-10 38.01165 6 GGCATTC 125 1.3278623E-10 38.01165 7 CGCAGAA 185 0.0 35.95697 9 TTAGGCA 135 3.0195224E-10 35.206547 4 CATTCCG 135 3.0377123E-10 35.195972 9 GCATTCC 140 4.474714E-10 33.938972 8 GCTTTGC 185 0.0 33.408676 1 TAGGCAT 145 6.511982E-10 32.778507 5 CTTTGCG 190 0.0 32.519733 2 GGATAAT 30 0.0039468384 31.666864 20-21 TTTGCGT 185 9.094947E-12 30.829515 3 TGGGCAA 65 0.005821191 29.239729 6 TTGCGTT 205 3.092282E-11 27.821758 4 GTAAGCG 35 0.008370637 27.15118 94-95 TGGGATA 55 9.456429E-5 25.909254 18-19 TAAAGGT 185 8.794814E-9 25.691263 5 GAGTACT 1370 0.0 25.317978 12-13 AAAGGTT 210 1.2405508E-9 24.88858 6 >>END_MODULE