##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936853_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 166635 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.94679389083926 31.0 31.0 34.0 30.0 34.0 2 32.027203168602036 33.0 31.0 34.0 30.0 34.0 3 32.10850661625709 33.0 31.0 34.0 30.0 34.0 4 35.75643172202719 37.0 35.0 37.0 35.0 37.0 5 35.697884598073635 37.0 35.0 37.0 33.0 37.0 6 35.855522549284366 37.0 35.0 37.0 35.0 37.0 7 35.8318420499895 37.0 35.0 37.0 35.0 37.0 8 35.83190806253188 37.0 35.0 37.0 35.0 37.0 9 37.382764725297804 39.0 37.0 39.0 34.0 39.0 10-11 37.272844840519696 39.0 37.0 39.0 33.5 39.0 12-13 37.25759594322921 39.0 37.0 39.0 34.0 39.0 14-15 38.13571878656945 40.0 37.0 41.0 33.0 41.0 16-17 38.11187025534852 40.0 37.0 41.0 33.5 41.0 18-19 38.39332973264921 40.0 37.5 41.0 34.0 41.0 20-21 38.473937048039126 40.0 38.0 41.0 34.0 41.0 22-23 38.38172652804033 40.0 37.5 41.0 34.0 41.0 24-25 38.14169892279533 40.0 37.0 41.0 33.5 41.0 26-27 37.8331052900051 39.5 36.5 41.0 33.0 41.0 28-29 37.77125153779218 39.5 36.5 41.0 33.0 41.0 30-31 37.75365019353677 39.0 36.5 41.0 33.0 41.0 32-33 37.368154949440395 39.0 35.0 41.0 33.0 41.0 34-35 37.033006271191525 39.0 35.0 41.0 33.0 41.0 36-37 36.72361448675248 39.0 35.0 40.5 32.0 41.0 38-39 36.42334743601285 38.0 35.0 40.0 31.0 41.0 40-41 36.36011942269031 39.0 35.0 40.0 31.0 41.0 42-43 35.90380472289735 39.0 35.0 40.0 30.0 41.0 44-45 35.42364749302367 38.5 35.0 40.0 29.0 41.0 46-47 34.75519548714256 38.0 35.0 40.0 24.0 41.0 48-49 34.06877906802292 38.0 34.0 40.0 19.5 41.0 50-51 33.665472439763555 38.0 34.0 40.0 11.0 41.0 52-53 33.554121283043784 38.0 34.0 40.0 8.0 41.0 54-55 33.357932007081345 38.0 33.5 40.0 4.0 41.0 56-57 32.94645782698713 38.0 33.0 40.0 2.0 41.0 58-59 32.63887238575329 38.0 32.5 40.0 2.0 41.0 60-61 32.05748792270531 37.0 31.0 39.5 2.0 41.0 62-63 31.811555195487145 36.0 31.0 39.0 2.0 40.0 64-65 31.470639421490084 36.0 31.0 39.0 2.0 40.0 66-67 30.84873826026945 35.5 30.0 38.5 2.0 40.0 68-69 30.427452215921022 35.0 30.0 37.5 2.0 39.0 70-71 29.766435622768327 34.5 29.5 37.0 2.0 39.0 72-73 29.158346085756293 34.0 29.0 36.5 2.0 38.0 74-75 28.73473760014403 34.0 29.0 36.0 2.0 37.0 76-77 27.36421820145828 32.0 26.5 34.0 2.0 36.0 78-79 28.069748252167912 34.0 28.0 35.0 2.0 36.0 80-81 27.747751072703814 34.0 26.5 35.0 2.0 36.0 82-83 27.366939718546526 33.0 26.0 35.0 2.0 35.5 84-85 27.00641521889159 33.0 26.0 35.0 2.0 35.0 86-87 26.904116782188616 33.0 26.0 35.0 2.0 35.0 88-89 26.429483601884357 33.0 25.0 35.0 2.0 35.0 90-91 25.87257778978006 32.0 24.0 34.0 2.0 35.0 92-93 25.436210880067215 32.0 21.5 34.0 2.0 35.0 94-95 24.73430551804843 32.0 18.5 34.0 2.0 35.0 96-97 23.963738110240946 31.0 4.5 34.0 2.0 35.0 98-99 23.339604524859723 31.0 2.0 34.0 2.0 35.0 100-101 20.10424880727338 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21221651177026857 1101 2 0.18465477726038415 1101 3 0.3689505580136796 1101 4 0.245564450416488 1101 5 0.1800837895948817 1101 6 0.17130680855652258 1101 7 0.37116666445614754 1101 8 0.27688229054533764 1101 9 0.5028676426502585 1101 10-11 0.2836225099618659 1101 12-13 0.44211302081786386 1101 14-15 0.6215428754276076 1101 16-17 0.39867213153814873 1101 18-19 0.4321015516642035 1101 20-21 0.23097907294555853 1101 22-23 0.3821993055367372 1101 24-25 0.14343839342922138 1101 26-27 0.34206252454487895 1101 28-29 0.28574293400536277 1101 30-31 0.42506835858642944 1101 32-33 0.22184773913895128 1101 34-35 -0.025757063971504124 1101 36-37 -0.26240080658374865 1101 38-39 -0.593196075045995 1101 40-41 -0.14753009863444078 1101 42-43 -0.348420511570275 1101 44-45 -0.011212097867115745 1101 46-47 0.2097020206875868 1101 48-49 0.28517315300839385 1101 50-51 -0.25581959283167066 1101 52-53 -0.07958888653035245 1101 54-55 0.009648254414862834 1101 56-57 0.4185970309416689 1101 58-59 0.03916413060772328 1101 60-61 0.10749047585650118 1101 62-63 -0.06675529426290083 1101 64-65 -0.23616186891841195 1101 66-67 -0.1776650482306792 1101 68-69 -0.054474233250036974 1101 70-71 0.3553923740426477 1101 72-73 0.1759682043203945 1101 74-75 0.003111950968971655 1101 76-77 0.2752947464923814 1101 78-79 0.31104983729844804 1101 80-81 0.6204697365544298 1101 82-83 0.6007563995050269 1101 84-85 0.4636896268747499 1101 86-87 0.23929830067844193 1101 88-89 0.15183929390460094 1101 90-91 0.34152643301390384 1101 92-93 0.3655192500740476 1101 94-95 0.8473848585188506 1101 96-97 1.1536630374984433 1101 98-99 0.9562007515950413 1101 100-101 0.59552038109263 1102 1 0.19015768824086265 1102 2 0.26112536549567267 1102 3 0.14652408742544054 1102 4 0.16431445041648374 1102 5 0.17640731900664974 1102 6 0.2168950438506414 1102 7 0.2424901938679156 1102 8 0.23680876113357385 1102 9 0.30360293676790207 1102 10-11 0.3316004511383355 1102 12-13 0.08402478552374504 1102 14-15 0.17926346366289891 1102 16-17 0.47587801389109075 1102 18-19 0.538903022252434 1102 20-21 0.31645701412202243 1102 22-23 0.44157430553674004 1102 24-25 0.7313060404880503 1102 26-27 0.4030919363095862 1102 28-29 0.689603228123012 1102 30-31 0.8015389468217293 1102 32-33 0.7431712685507179 1102 34-35 0.526081171322609 1102 36-37 0.5855771345927181 1102 38-39 0.8290465720128282 1102 40-41 0.9263669601890925 1102 42-43 1.44293978254737 1102 44-45 0.997611431544648 1102 46-47 0.8343343736287636 1102 48-49 1.085173153008391 1102 50-51 0.9849892306977353 1102 52-53 1.214712584057878 1102 54-55 0.8991703132383932 1102 56-57 0.8605087956475472 1102 58-59 1.014899424725371 1102 60-61 1.145174299385907 1102 62-63 2.0032814704429853 1102 64-65 1.5121837193168766 1102 66-67 1.2111217164752048 1102 68-69 1.351775766749963 1102 70-71 1.182414432866178 1102 72-73 1.5658579102027481 1102 74-75 1.7266413627336732 1102 76-77 1.5416550406100278 1102 78-79 1.8533292490631545 1102 80-81 1.951719736554427 1102 82-83 1.7198740465638487 1102 84-85 1.6822558033453383 1102 86-87 1.389298300678444 1102 88-89 1.6665451762575465 1102 90-91 2.1762690800727285 1102 92-93 1.790886897132868 1102 94-95 1.8608039761659079 1102 96-97 2.7064203904396216 1102 98-99 2.815759575124453 1102 100-101 2.8613292046220415 1103 1 0.14704310136565013 1103 2 0.23320969055517793 1103 3 -0.06027632059903709 1103 4 -0.02470289062397768 1103 5 0.2713410150284119 1103 6 0.36595998774727434 1103 7 -0.07644894248026901 1103 8 0.07424500730834893 1103 9 0.30322891432655297 1103 10-11 0.33017236545320117 1103 12-13 0.3499887433612159 1103 14-15 0.42302408930044066 1103 16-17 0.27533398124913333 1103 18-19 0.14396932623068182 1103 20-21 0.3144508937548025 1103 22-23 0.0779904905078368 1103 24-25 0.19354677493211625 1103 26-27 0.32407119506511606 1103 28-29 0.4478997259128832 1103 30-31 0.5554151793956734 1103 32-33 0.025388201566698854 1103 34-35 0.3320825314042182 1103 36-37 0.11333980035266222 1103 38-39 0.4644086937401326 1103 40-41 -0.11584657262287124 1103 42-43 0.10759466184013178 1103 44-45 0.20815206398259534 1103 46-47 0.2322092461211156 1103 48-49 -0.21222569092223864 1103 50-51 -0.08605803213802687 1103 52-53 -0.06027891543209307 1103 54-55 -0.38784090743484967 1103 56-57 0.15335136620177536 1103 58-59 -0.04732430869862725 1103 60-61 0.5761501579374269 1103 62-63 0.46173097741339575 1103 64-65 -0.5038822446409554 1103 66-67 -0.30094900776825284 1103 68-69 -0.5485132505910748 1103 70-71 -0.27569505370301783 1103 72-73 0.004382221661433761 1103 74-75 0.22218709547763638 1103 76-77 0.24677234764845224 1103 78-79 0.06650503961058618 1103 80-81 0.2468064417567426 1103 82-83 0.41072749777092454 1103 84-85 0.5570602916146328 1103 86-87 0.31935610414665305 1103 88-89 0.2463479644248352 1103 90-91 0.18041732896766405 1103 92-93 0.08723167781971242 1103 94-95 0.09834222846104623 1103 96-97 0.5352201184232968 1103 98-99 0.32968341055457984 1103 100-101 0.505021826179334 1104 1 0.3666282764761526 1104 2 0.16112536549567835 1104 3 -0.01818179492750005 1104 4 0.1760791562988402 1104 5 0.25287790724194537 1104 6 0.0992479850271124 1104 7 -0.10456862966150027 1104 8 0.007396996427694091 1104 9 0.27419117206201804 1104 10-11 0.3433651570206919 1104 12-13 0.4016718443472769 1104 14-15 0.08514581660407572 1104 16-17 0.4582309550675632 1104 18-19 0.45949125754655284 1104 20-21 0.13704524941614693 1104 22-23 0.07098607024262549 1104 24-25 0.1107178051939286 1104 26-27 0.17662134807429908 1104 28-29 0.5366620516524208 1104 30-31 0.33683306446878447 1104 32-33 0.4784653861977759 1104 34-35 0.07902234779319883 1104 36-37 0.09145948753388922 1104 38-39 0.4761053955422412 1104 40-41 0.496955195483217 1104 42-43 0.7723515472532512 1104 44-45 0.6799643727211162 1104 46-47 0.25492260892288243 1104 48-49 0.42634962359662865 1104 50-51 1.1967539365800945 1104 52-53 0.9235361134696447 1104 54-55 0.8462291367678034 1104 56-57 1.1163911485887184 1104 58-59 0.9825464835489086 1104 60-61 1.2216448876212027 1104 62-63 1.1915167645606317 1104 64-65 1.347477836963943 1104 66-67 1.0581805400046136 1104 68-69 0.95471694322055 1104 70-71 1.3059438446308818 1104 72-73 1.1982108513792191 1104 74-75 1.0854648921454384 1104 76-77 1.2710668053159146 1104 78-79 1.7033292490631524 1104 80-81 1.875249148319135 1104 82-83 1.7581093406814965 1104 84-85 1.4910793327570993 1104 86-87 1.8775335947960912 1104 88-89 1.7930157644928393 1104 90-91 1.6703867271315502 1104 92-93 0.982063367721107 1104 94-95 0.775509858518852 1104 96-97 1.3711262727925622 1104 98-99 1.389288986889163 1104 100-101 1.6466233222691002 1105 1 0.47821030059014547 1105 2 0.3324808642169046 1105 3 0.45912912944224615 1105 4 0.29537035103760445 1105 5 0.5039191933215932 1105 6 0.39475401352547124 1105 7 0.2892567265679489 1105 8 0.4192347750173937 1105 9 0.6588459035198184 1105 10-11 0.41073819319168337 1105 12-13 0.36641426305389047 1105 14-15 0.5704947387816475 1105 16-17 0.44222803215926376 1105 18-19 0.38033524731637414 1105 20-21 0.33534630897040074 1105 22-23 0.5297730633007163 1105 24-25 0.33128777231121376 1105 26-27 0.027388611501407922 1105 28-29 0.698572902949465 1105 30-31 0.09804972504606013 1105 32-33 0.27751544100230063 1105 34-35 0.7663625012458866 1105 36-37 0.567509907701961 1105 38-39 0.6659848255751228 1105 40-41 0.5220739386326443 1105 42-43 0.2693007617216523 1105 44-45 0.5394090201453068 1105 46-47 0.36851413248883347 1105 48-49 0.5593967554928625 1105 50-51 0.7357564941248427 1105 52-53 0.8496230699913809 1105 54-55 1.088355552551512 1105 56-57 0.9599790185193058 1105 58-59 0.8086517082474742 1105 60-61 0.6968732398316533 1105 62-63 0.7303914448675357 1105 64-65 0.39627213729276534 1105 66-67 0.45500187723516206 1105 68-69 0.7818999903524464 1105 70-71 0.6467549516824 1105 72-73 0.8571987943825086 1105 74-75 1.321745491341641 1105 76-77 0.635096765125926 1105 78-79 0.8431538745655267 1105 80-81 1.5563781216475938 1105 82-83 1.4027750330453976 1105 84-85 1.6044699063778545 1105 86-87 1.086813828628756 1105 88-89 1.2943858777555377 1105 90-91 1.07594025288968 1105 92-93 1.198943163117523 1105 94-95 1.20377072808407 1105 96-97 0.48811567724999705 1105 98-99 0.8547255963155393 1105 100-101 1.3577486419621927 1106 1 0.22064846443299402 1106 2 0.20950684826630095 1106 3 1.2659351663302232E-4 1106 4 -0.03154353774919372 1106 5 -0.20312626957671398 1106 6 0.13447248902989628 1106 7 0.17774950469283368 1106 8 -0.05880557927714136 1106 9 0.34317829353783225 1106 10-11 0.018478279192642333 1106 12-13 0.10668402673501731 1106 14-15 0.30811831921458577 1106 16-17 0.05022538597602022 1106 18-19 0.15985672917899052 1106 20-21 0.11102714986863305 1106 22-23 -0.24304107907864392 1106 24-25 -0.035888529647692735 1106 26-27 -0.3648161736799693 1106 28-29 -0.008987095580430093 1106 30-31 0.2987369976396863 1106 32-33 0.21393353795551917 1106 34-35 0.4670313975669558 1106 36-37 0.3147626549547127 1106 38-39 0.30060939832678457 1106 40-41 0.741560138051959 1106 42-43 0.8828109973054623 1106 44-45 0.5707405648547805 1106 46-47 1.0121798313384787 1106 48-49 1.1511494843693413 1106 50-51 0.7370428332038301 1106 52-53 0.8046012022270475 1106 54-55 0.924126804710717 1106 56-57 0.8769550191073421 1106 58-59 0.971042828832573 1106 60-61 0.6874645882825305 1106 62-63 1.4691359779264488 1106 64-65 1.4584941961703457 1106 66-67 1.7882188275089703 1106 68-69 1.1816574235546966 1106 70-71 0.7499744746343637 1106 72-73 0.51681509781152 1106 74-75 0.7912776314423411 1106 76-77 0.801052882587058 1106 78-79 0.3969596006120568 1106 80-81 0.44077299099229705 1106 82-83 -0.03755720996242928 1106 84-85 0.3955868162238616 1106 86-87 0.6330852829269631 1106 88-89 0.6018392939046038 1106 90-91 0.383926580942898 1106 92-93 0.9165917352811483 1106 94-95 1.0184092668028732 1106 96-97 0.6750942505161959 1106 98-99 1.311008443902736 1106 100-101 1.1929686059447029 1107 1 -0.06564063108687179 1107 2 0.07608334868895383 1107 3 0.1334148437279623 1107 4 0.40717159327363106 1107 5 0.3861552181663157 1107 6 0.146306808556524 1107 7 0.14938095017043196 1107 8 0.1613465762596249 1107 9 -0.030346643064028456 1107 10-11 0.06353322424758545 1107 12-13 0.023184449389290762 1107 14-15 -0.07720712457238932 1107 16-17 0.3140292743952955 1107 18-19 0.14915512309277545 1107 20-21 0.0569612158026942 1107 22-23 0.11300287696531797 1107 24-25 0.29861696485779987 1107 26-27 0.673848238830594 1107 28-29 0.5485107911482245 1107 30-31 0.709354072872145 1107 32-33 0.9586334534246603 1107 34-35 0.7426357931713525 1107 36-37 0.9246527648448151 1107 38-39 1.0092146392397225 1107 40-41 1.1730056156512774 1107 42-43 0.7532759170011545 1107 44-45 0.4016450449900262 1107 46-47 0.7041663064018735 1107 48-49 1.0208874387226814 1107 50-51 0.8650732643111851 1107 52-53 0.5978218277553609 1107 54-55 0.7186661115577166 1107 56-57 0.6896684595130935 1107 58-59 0.8066641306077216 1107 60-61 0.3838297615707873 1107 62-63 0.5110125628799551 1107 64-65 0.4914274167958723 1107 66-67 0.6245670946264639 1107 68-69 0.8789186238928188 1107 70-71 0.9133388026140778 1107 72-73 0.6169503471775393 1107 74-75 0.5288262366832548 1107 76-77 0.49199117506381285 1107 78-79 1.0455141230127332 1107 80-81 1.0645768794115718 1107 82-83 1.1971849709335949 1107 84-85 1.197886055446176 1107 86-87 0.8578697292498703 1107 88-89 1.4589821510474614 1107 90-91 1.593580004442476 1107 92-93 1.9553406786454737 1107 94-95 1.57408128709028 1107 96-97 1.9719666089270156 1107 98-99 2.2987007515950424 1107 100-101 2.159984666806917 1108 1 -0.06447840348396738 1108 2 0.058101104943993676 1108 3 -0.0784011369015758 1108 4 -0.08596803545922427 1108 5 -0.015749543738451166 1108 6 0.05591132833053791 1108 7 -0.04232203610882124 1108 8 -0.5953281614320574 1108 9 -0.11344591667177895 1108 10-11 -0.027588648230221224 1108 12-13 -0.05612144245897355 1108 14-15 -0.05200938445939585 1108 16-17 0.207782301029674 1108 18-19 0.26156835957380764 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-2.0147251232733083 2316 90-91 -1.5681553154523513 2316 92-93 -1.6096724677173633 2316 94-95 -2.1487232703155037 2316 96-97 -1.625228833667201 2316 98-99 -1.9825262422699907 2316 100-101 -2.066097717066878 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 2.0 5 7.0 6 19.0 7 48.0 8 70.0 9 170.0 10 316.0 11 559.0 12 859.0 13 1170.0 14 1404.0 15 2105.0 16 2777.0 17 3835.0 18 3145.0 19 1519.0 20 1151.0 21 1191.0 22 1209.0 23 1320.0 24 1526.0 25 1794.0 26 2055.0 27 2405.0 28 3035.0 29 3801.0 30 4639.0 31 5730.0 32 7217.0 33 9317.0 34 12931.0 35 17966.0 36 24514.0 37 31761.0 38 14669.0 39 398.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.007011477692934 21.359794458051194 16.457763050473034 14.175431013782836 2 14.40317363149145 27.377372871692383 34.165151269632645 24.05430222718352 3 28.234764605275004 24.260209439793563 35.490143127194166 12.01488282773727 4 11.100309058721157 19.587721667116753 43.327032136105856 25.98493713805623 5 8.101539292465569 42.80973384943139 36.63336033846431 12.455366519638732 6 22.14600774147088 40.61271641611906 17.39310468989108 19.84817115251898 7 19.738350286554446 40.82215620967984 22.184415038857384 17.255078464908333 8 23.783718906592252 25.14357727968314 21.721127014132684 29.35157679959192 9 19.684940138626338 24.11018093437753 29.009511807243378 27.195367119752756 10-11 24.05700293476891 25.22040774682967 27.789680896394852 22.932908422006566 12-13 30.63162000780148 26.91511387163561 26.1643712305338 16.288894890029106 14-15 23.08488612836439 28.548624238605335 34.28001320250848 14.086476430521799 16-17 15.478440903771718 31.360758544123385 33.53647192966664 19.624328622438263 18-19 14.985147177963812 34.45224592672608 34.58607135355717 15.976535541752934 20-21 19.018684718258932 26.212316250933938 32.37464255386822 22.394356476938913 22-23 16.542641613605948 27.032160496420275 35.411653153937095 21.01354473603668 24-25 22.877172344045878 27.31744207332627 32.88668549289815 16.9187000897297 26-27 20.18514906450209 23.125628291497247 41.60272472205134 15.086497921949316 28-29 15.197078627410972 33.607187008653696 35.69649651331661 15.499237850618721 30-31 17.96822253229509 27.03905417773177 34.37307767325321 20.61964561671993 32-33 18.12766698075273 27.738100239465613 37.19099033135079 16.94324244843087 34-35 21.661715725987936 26.59405287004531 34.31511987277583 17.429111531190927 36-37 19.244099686416902 24.969316868970274 39.40494230971207 16.381641134900747 38-39 17.70829894571308 28.789265660497403 37.25927415361438 16.243161240175144 40-41 17.422432935245755 30.808077777110967 34.72453939866771 17.044949888975573 42-43 15.995739190446184 30.751342755123474 31.238335283703904 22.014582770726438 44-45 20.019803762714915 25.70468388993909 32.464968343985355 21.810544003360636 46-47 23.341804968358893 30.05890136796402 32.322838307793404 14.276455355883686 48-49 20.489338846446984 27.782912148253946 33.828833424351714 17.898915580947353 50-51 13.77273000060013 30.264358158794934 38.12008641901218 17.84282542159275 52-53 19.19824766705674 33.904941938968406 28.503015572958862 18.393794821015995 54-55 16.405209146012123 27.185380783772427 32.555962311708576 23.85344775850687 56-57 21.405552354889817 27.96041576647943 29.932726007009457 20.701305871621297 58-59 26.39121433072284 27.323791520388873 28.44480451285744 17.840189636030846 60-61 19.986497434512557 28.9020913973655 35.59876376511537 15.512647403006572 62-63 17.469138395616664 29.370585305255325 34.39546062857451 18.764815670553496 64-65 16.44372430761845 34.451945869715246 31.293845830707834 17.810483991958474 66-67 19.525909922885347 29.10222942359048 27.77537732169112 23.59648333183305 68-69 16.68917094247907 28.81747532031086 31.743031175923424 22.750322561286644 70-71 22.639476929632153 29.53943282032509 28.917101508418273 18.903988741624485 72-73 20.54040267650854 24.758304077774778 36.51393764815315 18.18735559756354 74-75 16.44522459267261 35.12497374501155 30.734539562516876 17.69526209979896 76-77 18.284874126083956 28.821076004440844 29.852371950670626 23.041677918804574 78-79 18.483322731255324 30.141207676704635 31.6523038515549 19.723165740485136 80-81 21.369460197437512 28.099138836378913 30.24784709094728 20.283553875236297 82-83 18.08249186034299 28.009422496961694 34.505243889630755 19.40284175306456 84-85 17.556862509752143 30.162935845886096 32.02514553201705 20.255056112344715 86-87 17.07669826295433 31.78112444376431 30.28802731743617 20.854149975845193 88-89 16.97362498874786 30.886668467008732 27.660155429531613 24.479551114711796 90-91 18.55072463768116 25.2236925015753 31.221232034086476 25.004350826657063 92-93 21.371324665660474 29.019500763647383 31.876952251784594 17.73222231890755 94-95 19.076424520658925 27.487322591292347 32.61439673537973 20.821856152669007 96-97 13.987199529509194 29.006898331377958 35.697289576888345 21.30861256222451 98-99 18.047883241465605 31.239029132622598 27.958796047637197 22.754291578274607 100-101 16.130213131134308 27.321172986386372 31.545088202022992 25.003525680456328 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2963.0 1 1777.5 2 539.5 3 439.0 4 337.0 5 290.5 6 407.0 7 688.5 8 871.5 9 859.0 10 666.0 11 454.5 12 385.5 13 361.0 14 341.0 15 345.5 16 376.5 17 380.0 18 424.0 19 499.0 20 607.5 21 713.0 22 792.0 23 875.0 24 1028.5 25 1384.5 26 2155.0 27 3218.5 28 3479.0 29 3565.0 30 3813.0 31 4006.5 32 4126.5 33 3861.0 34 4558.0 35 5455.5 36 5772.5 37 5627.5 38 4066.0 39 2491.0 40 2043.5 41 1811.0 42 1578.0 43 1422.5 44 1362.0 45 1487.0 46 2651.0 47 10935.0 48 27603.0 49 26425.5 50 9904.0 51 2590.0 52 830.5 53 404.5 54 321.5 55 266.5 56 205.5 57 179.0 58 172.5 59 157.0 60 164.0 61 173.5 62 140.0 63 82.5 64 38.5 65 32.0 66 28.5 67 23.5 68 22.0 69 15.5 70 11.0 71 11.5 72 6.5 73 2.0 74 2.0 75 3.0 76 3.0 77 1.5 78 1.5 79 1.5 80 1.0 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030605815104869927 2 0.007201368259969395 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.007201368259969395 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 9.001710324961743E-4 22-23 0.0012002280433282323 24-25 0.01410267950910673 26-27 0.0075014252708014515 28-29 6.001140216641161E-4 30-31 0.004500855162480871 32-33 0.008401596303297627 34-35 0.0 36-37 0.0075014252708014515 38-39 0.01770336363909143 40-41 0.003000570108320581 42-43 0.0 44-45 0.0 46-47 3.000570108320581E-4 48-49 0.0012002280433282323 50-51 0.003000570108320581 52-53 0.0 54-55 0.003000570108320581 56-57 0.0018003420649923487 58-59 0.0 60-61 0.0 62-63 0.0024004560866564646 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.002100399075824407 72-73 0.0 74-75 0.0 76-77 0.0 78-79 6.001140216641161E-4 80-81 0.0 82-83 0.0075014252708014515 84-85 0.003000570108320581 86-87 9.001710324961743E-4 88-89 0.0 90-91 0.0 92-93 3.000570108320581E-4 94-95 0.0 96-97 3.000570108320581E-4 98-99 3.000570108320581E-4 100-101 3.000570108320581E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 166635.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 19.70834458547124 #Duplication Level Percentage of deduplicated Percentage of total 1 77.75037300934807 15.323311429171543 2 10.31028287810968 4.063972154709395 3 3.5199902560823357 2.081195427131155 4 1.8939739959197344 1.493083685900321 5 1.0779208915684662 1.0622018183454855 6 0.770378490301757 0.9109730848861284 7 0.5572302914040377 0.7687460617517328 8 0.44761121768521056 0.7057340894770006 9 0.3105873755366767 0.5509046718876587 >10 2.4846990042934136 10.587211570198338 >50 0.3897567065558296 5.357817985417229 >100 0.38062178374592737 15.200288054730398 >500 0.03349471696964161 4.4846520838959405 >1k 0.06698943393928322 28.435802802532486 >5k 0.006089948539934838 8.974105079965193 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9648 5.789900081015393 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5306 3.1842049989498 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4405 2.6435022654304317 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3253 1.9521709124733702 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3123 1.874156089657035 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2934 1.7607345395625167 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2800 1.6803192606595254 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2696 1.6179074024064573 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2664 1.5987037537132056 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2621 1.5728988507816486 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2351 1.410868064932337 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2316 1.389864074174093 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2194 1.316650163531071 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1910 1.1462177813784618 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1750 1.0501995379122033 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1744 1.0465988537822186 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1676 1.0057911003090587 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1623 0.9739850571608606 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1616 0.9697842590092117 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1376 0.8257568938098238 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1111 0.666726678068833 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1099 0.6595253098088637 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1070 0.6421220031806043 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1052 0.6313199507906502 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 915 0.5491043298226663 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 910 0.5461037597143458 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 894 0.53650193536772 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 825 0.49509406787289584 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 675 0.4050769646232784 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 586 0.3516668166951721 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 575 0.3450655624568668 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 543 0.32586191376361506 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 526 0.3156599753953251 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 514 0.3084586071353557 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 510 0.30605815104869927 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 463 0.2778527920304858 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 456 0.273651993878837 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 455 0.2730518798571729 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 435 0.2610495994238905 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 434 0.2604494854022264 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 422 0.25324811714225703 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 421 0.25264800312059293 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 419 0.2514477750772647 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 416 0.24964743301227232 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 388 0.23284424040567708 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 380 0.22804332823236415 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 374 0.22444264410237946 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 356 0.2136405917124254 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 339 0.2034386533441354 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 339 0.2034386533441354 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 337 0.20223842530080716 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 336 0.20163831127914303 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 335 0.20103819725747893 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 317 0.19023614486752483 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 311 0.18663546073754012 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 305 0.18303477660755543 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 301 0.18063432052089898 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 290 0.17403306628259368 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 274 0.16443124193596784 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 271 0.16263089987097548 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 270 0.16203078584931138 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 261 0.15662975965433432 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 259 0.1554295316110061 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 254 0.1524289615026855 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 250 0.15002850541602905 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 244 0.14642782128604434 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 239 0.14342725117772376 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 238 0.14282713715605966 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 236 0.14162690911273143 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 234 0.14042668106940318 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 234 0.14042668106940318 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 227 0.13622588291775437 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 226 0.13562576889609024 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 222 0.13322531280943378 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 221 0.13262519878776968 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 214 0.12842440063612084 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 212 0.12722417259279264 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 206 0.12362348846280792 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 203 0.12182314639781558 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 200 0.12002280433282322 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 199 0.11942269031115912 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 197 0.11822246226783088 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 196 0.11762234824616677 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 195 0.11702223422450267 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 194 0.11642212020283854 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 194 0.11642212020283854 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 188 0.11282143607285385 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 188 0.11282143607285385 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 182 0.10922075194286915 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 182 0.10922075194286915 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 182 0.10922075194286915 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 179 0.1074204098778768 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 178 0.1068202958562127 No Hit ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT 177 0.10622018183454857 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 176 0.10562006781288445 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 175 0.10501995379122034 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 174 0.10441983976955621 No Hit GCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTA 174 0.10441983976955621 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 174 0.10441983976955621 No Hit ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC 171 0.10261949770456387 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 171 0.10261949770456387 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 170 0.10201938368289976 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.001140216641161E-4 0.0 14-15 0.0 0.0 0.0 6.001140216641161E-4 0.0 16-17 0.0 0.0 0.0 0.0015002850541602905 0.0 18-19 0.0 0.0 0.0 0.0018003420649923484 0.0 20-21 0.0 0.0 0.0 0.0024004560866564646 0.0 22-23 0.0 0.0 0.0 0.004800912173312929 0.0 24-25 0.0 0.0 0.0 0.009001710324961743 0.0 26-27 0.0 0.0 0.0 0.015302907552434963 0.0 28-29 0.0 0.0 0.0 0.031806043148198154 0.0 30-31 0.0 0.0 0.0 0.05431031896060252 0.0 32-33 0.0 0.0 0.0 0.08611636210880066 0.0 34-35 0.0 0.0 0.0 0.11762234824616677 0.0 36-37 0.0 0.0 0.0 0.1461277642752123 0.0 38-39 0.0 0.0 0.0 0.1947370000300057 0.0 40-41 0.0 0.0 0.0 0.2448465208389594 0.0 42-43 0.0 0.0 0.0 0.29465598463708104 0.0 44-45 0.0 0.0 0.0 0.3372640801752333 0.0 46-47 0.0 0.0 0.0 0.3987757673958052 0.0 48-49 0.0 0.0 0.0 0.4377831788039728 0.0 50-51 0.0 0.0 0.0 0.4674888228763465 0.0 52-53 0.0 0.0 0.0 0.5380022204218802 0.0 54-55 0.0 0.0 0.0 0.6745281603504666 0.0 56-57 0.0 0.0 0.0 0.8962702913553575 0.0 58-59 0.0 0.0 0.0 1.0928076334503556 0.0 60-61 0.0 0.0 0.0 1.343655294505956 0.0 62-63 0.0 0.0 0.0 1.4747802082395656 0.0 64-65 0.0 0.0 0.0 1.627809283763915 0.0 66-67 0.0 0.0 0.0 1.9794761004590873 0.0 68-69 0.0 0.0 0.0 2.4763705103969755 0.0 70-71 0.0 0.0 0.0 2.919854772406757 0.0 72-73 0.0 0.0 0.0 3.3240315659975392 0.0 74-75 0.0 0.0 0.0 3.8086236384913135 0.0 76-77 0.0 0.0 0.0 4.154289314969844 0.0 78-79 0.0 0.0 0.0 4.543463258019024 0.0 80-81 0.0 0.0 0.0 4.880727338194257 0.0 82-83 0.0 0.0 0.0 5.2633000270051316 0.0 84-85 0.0 0.0 0.0 5.954031265940529 0.0 86-87 0.0 0.0 0.0 6.8055930626819094 0.0 88-89 0.0 0.0 0.0 7.777177663756113 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAAGCG 35 7.904964E-8 47.494896 94-95 AGGCATT 80 2.2489621E-7 41.558033 6 GGCATTC 80 2.2489621E-7 41.558033 7 CTTTAGG 80 2.2489621E-7 41.558033 2 GCTTTGC 145 0.0 39.30612 1 CCTTTAG 85 3.6188248E-7 39.11345 1 TTAGGCA 90 5.6638237E-7 36.94048 4 CTTTGCG 155 0.0 36.770245 2 TTTGCGT 160 1.8189894E-12 35.621174 3 CCCCCGA 35 2.0515094E-4 33.924927 92-93 CATTCCG 100 1.2914552E-6 33.24643 9 TAGGCAT 100 1.2914552E-6 33.24643 5 TTGCGTT 175 3.6379788E-12 32.567932 4 GGGCTTG 90 2.4024524E-5 31.663267 7 GCATTCC 115 3.8417584E-6 28.909937 8 ATGGGCT 105 6.876064E-5 27.139942 5 TCTCTTG 35 0.008387565 27.13994 52-53 GGGGTGA 140 5.7615216E-7 27.13994 9 TATTATA 70 0.008375739 27.13994 2 ACTTTCT 70 0.008375739 27.13994 8 >>END_MODULE