##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936852_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 169709 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.856896216464655 33.0 31.0 34.0 30.0 34.0 2 31.865399006534716 33.0 31.0 34.0 30.0 34.0 3 31.868857868468968 33.0 31.0 34.0 30.0 34.0 4 35.42330695484624 37.0 35.0 37.0 33.0 37.0 5 35.49586645375319 37.0 35.0 37.0 33.0 37.0 6 35.662074492219034 37.0 35.0 37.0 33.0 37.0 7 35.65916362715 37.0 35.0 37.0 33.0 37.0 8 35.658980961528265 37.0 35.0 37.0 33.0 37.0 9 37.19300685290703 39.0 37.0 39.0 34.0 39.0 10-11 37.071902491912624 39.0 37.0 39.0 33.5 39.0 12-13 37.08186660695661 39.0 37.0 39.0 33.0 39.0 14-15 38.00336752912338 40.0 38.0 41.0 33.0 41.0 16-17 38.00753937622636 40.0 38.0 41.0 33.0 41.0 18-19 38.059351595967215 40.0 38.0 41.0 33.5 41.0 20-21 37.983616072217735 40.0 37.5 41.0 33.5 41.0 22-23 37.880354017759814 40.0 37.0 41.0 33.5 41.0 24-25 37.7156632824423 40.0 37.0 41.0 33.0 41.0 26-27 37.61834669935006 39.5 36.5 41.0 33.0 41.0 28-29 37.61890648109411 39.5 36.5 41.0 33.0 41.0 30-31 37.502757661644345 39.0 36.5 41.0 33.0 41.0 32-33 37.139093978516165 39.0 35.0 41.0 33.0 41.0 34-35 36.8379431850992 39.0 35.0 41.0 32.0 41.0 36-37 36.5016587216942 38.5 35.0 40.5 31.0 41.0 38-39 36.20634144329411 38.0 35.0 40.0 30.5 41.0 40-41 36.19578219186961 39.0 35.0 40.0 30.5 41.0 42-43 35.8087284704995 38.0 35.0 40.0 30.0 41.0 44-45 35.347868999287016 38.0 35.0 40.0 28.0 41.0 46-47 34.66439316712726 38.0 35.0 40.0 24.0 41.0 48-49 34.056803115921966 38.0 34.0 40.0 19.5 41.0 50-51 33.15565467948076 37.5 33.5 39.5 14.0 40.5 52-53 33.43392218444514 38.0 33.5 39.5 13.5 40.5 54-55 33.847061735087706 38.0 34.0 40.0 10.0 41.0 56-57 33.59289136109458 38.0 34.0 40.0 7.5 41.0 58-59 33.41635387634126 38.0 34.0 40.0 3.5 41.0 60-61 32.86697228785745 38.0 33.0 40.0 2.0 41.0 62-63 32.6587305328533 37.0 33.0 40.0 2.0 41.0 64-65 32.347930869900836 37.0 32.5 39.5 2.0 41.0 66-67 31.690010547466546 36.0 31.5 39.0 2.0 40.5 68-69 31.18846378212116 36.0 31.0 38.5 2.0 40.0 70-71 30.471804677418405 35.0 30.5 37.5 2.0 39.0 72-73 29.984417443977634 35.0 29.5 37.0 2.0 39.0 74-75 29.665483268418292 35.0 30.0 36.5 2.0 38.5 76-77 29.23640466916899 34.5 29.0 36.0 2.0 37.0 78-79 28.689291669858406 34.0 29.0 35.5 2.0 37.0 80-81 28.14549611393621 34.0 28.0 35.0 2.0 36.5 82-83 27.68471324443606 34.0 27.0 35.0 2.0 36.0 84-85 27.28920976495059 34.0 26.0 35.0 2.0 36.0 86-87 27.21726307974238 34.0 26.0 35.0 2.0 35.0 88-89 26.676446151942443 34.0 25.0 35.0 2.0 35.0 90-91 26.206311981097052 33.0 24.0 35.0 2.0 35.0 92-93 25.806775126834758 33.0 21.5 35.0 2.0 35.0 94-95 25.29920039597193 33.0 19.0 35.0 2.0 35.0 96-97 24.77699768427131 33.0 9.5 35.0 2.0 35.0 98-99 24.38980254435534 33.0 2.0 35.0 2.0 35.0 100-101 22.318969530195808 29.5 2.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.38645381114710275 1101 2 0.5265087079730755 1101 3 0.6841321257719457 1101 4 0.40597874825815694 1101 5 0.3183557550150766 1101 6 0.2086519170832304 1101 7 0.17524796152871147 1101 8 0.12769620195913944 1101 9 0.6109587967848782 1101 10-11 0.5976949111308727 1101 12-13 0.5179512875715488 1101 14-15 0.483996031689351 1101 16-17 0.6703021650370644 1101 18-19 0.6706695921142725 1101 20-21 0.9136094667738703 1101 22-23 0.642589564421371 1101 24-25 0.51125381380227 1101 26-27 0.22551727218259288 1101 28-29 0.5621412114941435 1101 30-31 0.4371694091392655 1101 32-33 0.6157736840670296 1101 34-35 0.5897635050059193 1101 36-37 0.6893038448750701 1101 38-39 0.5449390593697316 1101 40-41 0.7432067346893589 1101 42-43 1.1896160357840344 1101 44-45 0.826928141615106 1101 46-47 1.6108817043919572 1101 48-49 1.2263193740214646 1101 50-51 1.7341524658754892 1101 52-53 1.8461524216948817 1101 54-55 1.5394208019017555 1101 56-57 1.8722279568200264 1101 58-59 1.6673624550845005 1101 60-61 1.6372898644719882 1101 62-63 2.037812432931858 1101 64-65 1.75363542087414 1101 66-67 1.458497713162334 1101 68-69 1.2959013011229636 1101 70-71 1.3615064385247102 1101 72-73 1.794868701468669 1101 74-75 1.5807766032772044 1101 76-77 1.4529267938247123 1101 78-79 1.9495177147877598 1101 80-81 2.3615784544736407 1101 82-83 2.438020730644954 1101 84-85 2.1828392048520406 1101 86-87 3.010768708895359 1101 88-89 2.706078740502729 1101 90-91 2.6950857185026607 1101 92-93 2.4698430762632633 1101 94-95 2.669830459944585 1101 96-97 3.3642413214628952 1101 98-99 2.7446993947852967 1101 100-101 2.2861880721100825 1102 1 -0.07602612464743785 1102 2 -0.030272993471545107 1102 3 0.09190366135247174 1102 4 -0.019583049494649174 1102 5 0.27548523602096253 1102 6 0.24904785073758973 1102 7 0.3015855752258787 1102 8 0.1172628151212578 1102 9 0.3807554795029091 1102 10-11 0.20299854944012452 1102 12-13 0.30721024958332777 1102 14-15 -0.3393720796001034 1102 16-17 -0.10925909767026809 1102 18-19 0.044432299444395085 1102 20-21 -0.014125953236835187 1102 22-23 0.05430706040853295 1102 24-25 -0.1082285296969232 1102 26-27 0.27102957822860674 1102 28-29 -0.09362390354063876 1102 30-31 -0.051393993749975664 1102 32-33 -0.21404841331123947 1102 34-35 -0.4312035896971267 1102 36-37 -0.39530022147056343 1102 38-39 -0.10811738792828152 1102 40-41 0.07714199418641243 1102 42-43 0.1999825419370609 1102 44-45 0.41681578206453906 1102 46-47 0.40014066640372903 1102 48-49 0.04647721243772196 1102 50-51 0.04598365902690915 1102 52-53 0.6292918010421218 1102 54-55 0.451238618916193 1102 56-57 0.2927402628660474 1102 58-59 -0.09939115647247831 1102 60-61 0.21293191904662478 1102 62-63 0.4342476924289187 1102 64-65 0.2853034251545026 1102 66-67 0.3838923092029951 1102 68-69 0.5104210978056791 1102 70-71 0.7639944000014367 1102 72-73 0.5476776902327138 1102 74-75 1.125419460420062 1102 76-77 0.6866079067193098 1102 78-79 0.28305169017566456 1102 80-81 0.5473061163248971 1102 82-83 0.8206116883763599 1102 84-85 0.3866246516150156 1102 86-87 0.5653433477396597 1102 88-89 0.6605664344567117 1102 90-91 -0.05434579567604558 1102 92-93 0.17091985528947973 1102 94-95 0.7850658799552832 1102 96-97 0.917879630719181 1102 98-99 1.1542432685145627 1102 100-101 0.924631089766585 1103 1 0.32005681035783695 1103 2 0.22629165982785437 1103 3 0.43528313866329427 1103 4 0.23263620682169517 1103 5 0.09614449482038623 1103 6 0.17852826541261635 1103 7 0.5505768223548131 1103 8 0.39723053502938654 1103 9 0.3934502464033969 1103 10-11 0.40276265646105003 1103 12-13 0.48858401580096 1103 14-15 0.35373425847178197 1103 16-17 0.4164873794543169 1103 18-19 0.48165683231422207 1103 20-21 0.3452228579867054 1103 22-23 0.6043101642635236 1103 24-25 0.3725896464778842 1103 26-27 0.43508383361379543 1103 28-29 0.25140061691376303 1103 30-31 0.36366901450627864 1103 32-33 0.5106862070872964 1103 34-35 0.6193116502308627 1103 36-37 0.4803127898895383 1103 38-39 0.9277166179069596 1103 40-41 0.311839989096093 1103 42-43 0.41695752486585747 1103 44-45 0.57140327973665 1103 46-47 0.41438736079816607 1103 48-49 0.634797406118274 1103 50-51 0.6438637260701796 1103 52-53 0.5136576834680966 1103 54-55 1.1280652375765712 1103 56-57 1.0728166176987486 1103 58-59 0.5525735837348549 1103 60-61 0.1085803143535955 1103 62-63 0.5404926486645607 1103 64-65 0.06235352137400696 1103 66-67 0.2144994232386317 1103 68-69 0.45551390306981787 1103 70-71 0.05369270529661563 1103 72-73 0.24100129815375126 1103 74-75 0.4279056482653658 1103 76-77 0.022119111635813482 1103 78-79 0.7148755153665505 1103 80-81 0.30399492382381155 1103 82-83 0.2539408832363783 1103 84-85 0.4646400467357559 1103 86-87 0.6333022526996182 1103 88-89 1.0780038917787031 1103 90-91 0.8024423351824801 1103 92-93 0.5360263175155602 1103 94-95 0.9091424830963852 1103 96-97 1.0424838892082242 1103 98-99 0.4660720598734329 1103 100-101 0.8471588692687249 1104 1 0.07004129108290158 1104 2 0.11579442225879077 1104 3 0.29976882989179643 1104 4 0.12086638870759714 1104 5 0.24739534838050758 1104 6 0.21533998556905232 1104 7 0.29034962016969956 1104 8 0.1509706802897952 1104 9 0.3470476143343646 1104 10-11 0.2956951786536095 1104 12-13 -0.0214414358099404 1104 14-15 -0.21015859645404333 1104 16-17 0.2474824753634337 1104 18-19 0.3056682545005742 1104 20-21 0.14317741754967983 1104 22-23 0.12453177950965255 1104 24-25 0.16986135794352464 1104 26-27 0.661479016430853 1104 28-29 0.4148030627514956 1104 30-31 0.5469206129915918 1104 32-33 0.18763697994717887 1104 34-35 0.31317843277478374 1104 36-37 0.19177843021482488 1104 38-39 0.2767140727458681 1104 40-41 0.2091644660965315 1104 42-43 0.5202072610381805 1104 44-45 0.6218719618398225 1104 46-47 0.06306201471833361 1104 48-49 0.46220754951637844 1104 50-51 0.7089050073415137 1104 52-53 1.1714266325028007 1104 54-55 0.6310138998150734 1104 56-57 0.3910548696076148 1104 58-59 0.3640919895949324 1104 60-61 0.05843753702415455 1104 62-63 0.6449218497322846 1104 64-65 0.7431685936938237 1104 66-67 0.5973754552704129 1104 68-69 0.7267132326371417 1104 70-71 1.247140467417168 1104 72-73 1.2274529711315871 1104 74-75 0.8613745165998381 1104 76-77 1.079866333685601 1104 78-79 1.086422476692519 1104 80-81 0.9546094871114121 1104 82-83 1.0958925872527665 1104 84-85 1.6366246516150156 1104 86-87 1.1243321117846037 1104 88-89 0.44708328838929745 1104 90-91 0.6759912829756409 1104 92-93 0.8928299676490283 1104 94-95 1.0856276777080929 1104 96-97 0.8504639003821062 1104 98-99 1.4548050662673724 1104 100-101 1.4639569324632156 1105 1 -0.1356298743981199 1105 2 0.42940388971441124 1105 3 0.33773173129463174 1105 4 0.152280523406084 1105 5 0.25407708496717163 1105 6 0.44012557163380706 1105 7 0.35956044391812014 1105 8 0.14079848429219055 1105 9 0.3932214059705643 1105 10-11 0.20020618191136919 1105 12-13 0.10019276404379696 1105 14-15 0.04609098801507372 1105 16-17 0.2044667184323785 1105 18-19 0.4295629423537761 1105 20-21 0.198143842654396 1105 22-23 0.43506631843658283 1105 24-25 0.21375804034496326 1105 26-27 0.6019085091507534 1105 28-29 0.43981802186325325 1105 30-31 0.4818650980657253 1105 32-33 0.5845853211479053 1105 34-35 -0.022504035146234003 1105 36-37 0.06648755859724531 1105 38-39 0.5075165459856876 1105 40-41 0.710627134802067 1105 42-43 0.5383410287398007 1105 44-45 0.17751985759144162 1105 46-47 0.11472081400030021 1105 48-49 0.5644780102570124 1105 50-51 0.494806875568365 1105 52-53 0.9978676936024584 1105 54-55 1.0191862287825586 1105 56-57 1.2597138683444058 1105 58-59 1.1312783468850114 1105 60-61 1.2701089684449371 1105 62-63 0.4715789868907194 1105 64-65 1.0341898023900598 1105 66-67 0.62933809073348 1105 68-69 0.9797382974599564 1105 70-71 0.6793424486684145 1105 72-73 1.4797799440722201 1105 74-75 2.049975673437814 1105 76-77 1.8275393607449715 1105 78-79 1.6973692222546504 1105 80-81 1.332027876846137 1105 82-83 0.9105658137669579 1105 84-85 0.7720544767596209 1105 86-87 0.9682919548801401 1105 88-89 0.9938886869665176 1105 90-91 1.0685183755891643 1105 92-93 0.7493056009180918 1105 94-95 0.344985433176479 1105 96-97 0.8192990842129682 1105 98-99 -0.13465042206452082 1105 100-101 -0.3797037283971001 1106 1 -0.25309645340315967 1106 2 -0.06939076292639612 1106 3 0.22271237268552824 1106 4 0.24702813097420773 1106 5 0.1836555786482279 1106 6 0.11782299292097775 1106 7 0.04010686805428776 1106 8 0.10124117550411427 1106 9 0.33185826768434623 1106 10-11 0.5702478211837843 1106 12-13 0.3951558731094664 1106 14-15 0.3391547618480857 1106 16-17 0.3391734172416463 1106 18-19 0.3032754122200956 1106 20-21 0.3702893609537554 1106 22-23 0.30881531398046036 1106 24-25 0.4319989637140864 1106 26-27 0.36341409757942245 1106 28-29 0.022238213257807615 1106 30-31 0.2283951586983548 1106 32-33 0.4838292396225867 1106 34-35 0.4313640341064442 1106 36-37 0.18335146392269053 1106 38-39 -0.1436632333992236 1106 40-41 -0.2102765104605524 1106 42-43 0.17969540086340174 1106 44-45 0.15100221568918215 1106 46-47 -0.5859877841442014 1106 48-49 -0.5693155466134598 1106 50-51 -1.3911782219551974 1106 52-53 -1.692978971603182 1106 54-55 -1.0314566231423399 1106 56-57 -0.8309246004991948 1106 58-59 -0.5666542997655881 1106 60-61 -0.576775391259936 1106 62-63 -0.3686029109834834 1106 64-65 -0.7030444733057557 1106 66-67 -1.225034032869413 1106 68-69 -0.7344117500234226 1106 70-71 -0.8254424150908086 1106 72-73 -0.6341436442103436 1106 74-75 -0.778901527234261 1106 76-77 -0.5400185324539457 1106 78-79 -0.6790096220729112 1106 80-81 -0.8732099053147202 1106 82-83 -0.7049466414890873 1106 84-85 0.004047317726818989 1106 86-87 0.36306147785479226 1106 88-89 0.4786757422663932 1106 90-91 -0.413931036347428 1106 92-93 -0.02012605953920854 1106 94-95 0.5518851336659232 1106 96-97 0.5776452015334428 1106 98-99 0.5913704706230405 1106 100-101 0.7875108234328358 1107 1 0.41882708303650773 1107 2 0.20611700290395518 1107 3 0.40052997523431344 1107 4 0.4664626192259007 1107 5 0.42945406530693475 1107 6 0.33415114903407783 1107 7 0.4917994208221046 1107 8 0.37423998076098286 1107 9 0.51656555561744 1107 10-11 0.5640351569065984 1107 12-13 0.23288984363913556 1107 14-15 0.29094687654342266 1107 16-17 0.4666923340078739 1107 18-19 0.9849361051711085 1107 20-21 0.5063706034866158 1107 22-23 0.17438679944441304 1107 24-25 0.49898422854881375 1107 26-27 0.6646685778661592 1107 28-29 0.5056511961331509 1107 30-31 0.4423537409365039 1107 32-33 0.4836500281530576 1107 34-35 0.04558147735615137 1107 36-37 0.291415980051724 1107 38-39 0.33343752327142084 1107 40-41 0.2630608053498875 1107 42-43 -0.014569832111511971 1107 44-45 -0.20694282612682713 1107 46-47 0.562917034653097 1107 48-49 -0.021645295717405588 1107 50-51 -0.17654261860992193 1107 52-53 -0.27203114205320134 1107 54-55 -0.5673188294346545 1107 56-57 -0.38781428588350764 1107 58-59 -0.3598840886942938 1107 60-61 -0.8937009189381513 1107 62-63 -0.3943496254398724 1107 64-65 -0.21654507067731998 1107 66-67 0.13052152268614492 1107 68-69 -0.5646785759891735 1107 70-71 -0.07904655686699513 1107 72-73 -0.19061516949907187 1107 74-75 -0.2987740879670362 1107 76-77 -0.21496875149018635 1107 78-79 -0.7474484910494184 1107 80-81 -0.6711788419933349 1107 82-83 -0.36315054431664606 1107 84-85 -0.284941133089589 1107 86-87 -0.32264142935349227 1107 88-89 0.4491854993352895 1107 90-91 0.19552734676686967 1107 92-93 0.42804200099444145 1107 94-95 -0.28704357999397345 1107 96-97 -0.5826096770010061 1107 98-99 -0.48800029799503974 1107 100-101 -0.15597782650742786 1108 1 0.1952466265188697 1108 2 0.09240260939329303 1108 3 0.34714382167837954 1108 4 0.22688518100669341 1108 5 -0.10029503863572131 1108 6 0.263372050750732 1108 7 0.24803409352536931 1108 8 0.40630940162497353 1108 9 0.2987198661248911 1108 10-11 0.19402949759703603 1108 12-13 0.3231935599190834 1108 14-15 0.606698621497209 1108 16-17 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13 1107.0 14 1359.0 15 1791.0 16 2533.0 17 3461.0 18 2845.0 19 1318.0 20 1267.0 21 1295.0 22 1432.0 23 1384.0 24 1697.0 25 1933.0 26 2175.0 27 2420.0 28 2931.0 29 3417.0 30 4144.0 31 5086.0 32 6546.0 33 8295.0 34 11640.0 35 18036.0 36 22409.0 37 29084.0 38 24146.0 39 2982.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.3692416516844 21.30095198797489 15.622034247988449 14.707772112352266 2 14.464385701234786 27.14702501989214 34.01644416939263 24.37214510948045 3 27.074910119644013 25.46295750574645 34.89774267696116 12.564389697648377 4 10.767317248574948 19.645372930212268 43.560300159747236 26.02700966146555 5 8.235925502176194 43.55280074545004 35.6949080572298 12.516365695143957 6 21.732770214472787 40.50916641106104 17.11535285373853 20.64271052072765 7 19.402210759027266 40.89255711127487 22.11997052321297 17.58526160648489 8 23.189610941066153 25.9478777799402 21.186225768593385 29.676285510400263 9 19.88017878834338 24.349297701461207 28.695175309282604 27.075348200912813 10-11 24.121613453901105 25.584583678535733 27.25305375133044 23.04074911623272 12-13 30.11745213766428 27.62218383146326 25.88103843727336 16.379325593599095 14-15 23.085267890043482 29.39347041049451 33.1841697988061 14.337091900655906 16-17 15.56471271381031 32.960169309174944 31.994541076044698 19.48057690097005 18-19 15.067051474424925 35.71902351592049 32.73745124802117 16.476473761633407 20-21 19.460976350285783 28.08753607296245 29.944022426402313 22.507465150349454 22-23 16.888478925870697 28.542168887736274 33.51143148476686 21.057920701626166 24-25 22.733165583363288 29.062976953039833 30.791351644401495 17.41250581919539 26-27 19.752980934394397 25.137371945776017 39.46206177244429 15.647585347385293 28-29 15.215495397814994 35.5095991797386 33.23325594277027 16.04164947967614 30-31 17.65539511327042 28.542260222916894 32.58723251298581 21.21511215082688 32-33 18.059516794342958 29.40070713022982 35.17678255745433 17.362993517972892 34-35 21.636456847886297 28.40155095402529 31.939222873036265 18.022769325052153 36-37 18.953645623101465 26.864207279166312 37.64241751054044 16.53972958719178 38-39 17.724961256754288 31.190522488760042 34.36010300105476 16.724413253430914 40-41 17.132881555686506 32.601649970536236 33.0061873895109 17.259281084266355 42-43 16.12523054984296 32.2925935310513 29.219577732862707 22.362598186243023 44-45 19.741494113348967 27.916809069803072 30.5929077345527 21.748789082295264 46-47 22.929452145603467 31.68746225122637 30.99804074657867 14.385044856591488 48-49 19.61268995751551 29.670200166166794 32.51595377970526 18.201156096612436 50-51 13.639350058188354 31.81415081831976 36.459201862027314 18.08729726146458 52-53 18.653416105148096 34.980716367075516 27.3530009693377 19.012866558438688 54-55 16.157422685272486 28.50597539128795 31.47157269470111 23.86502922873845 56-57 21.09079445145019 29.33937136863443 28.687051419547206 20.882782760368173 58-59 25.41005100041543 28.703422714188488 27.915877304373186 17.970648981022897 60-61 19.69229590739415 29.96549966560308 34.65029742175554 15.691907005247232 62-63 17.13229228049499 30.72362993517973 33.28933411903358 18.85474366529169 64-65 16.277418499477044 35.302800406581916 30.37402589750011 18.045755196440936 66-67 19.373764221581148 30.4498749119669 26.824237316603856 23.3521235498481 68-69 16.60860287312619 29.900201012726875 30.992212967384063 22.498983146762868 70-71 22.19975416142221 31.025477012324355 27.650944591993444 19.123824234259992 72-73 19.78698015645026 26.386470440918668 35.607018164140186 18.219531238490887 74-75 16.1979155923943 36.61915804547745 29.3947438983785 17.788182463749752 76-77 18.176246113951468 29.832770992028994 28.990879757186427 23.00010313683311 78-79 18.450947861013784 31.089782314649188 30.87436605440513 19.5849037699319 80-81 21.28615524311831 29.12348871010062 29.387052361901784 20.20330368487929 82-83 17.880749815080584 29.13298579288436 33.99452461712104 18.991739774914024 84-85 17.8181764610557 31.60152624523048 30.539342064556045 20.040955229157767 86-87 17.422170617566277 32.90170098900192 29.6102342248889 20.065894168542904 88-89 17.410911663277037 32.25277802340319 26.532849942523658 23.80346037079612 90-91 18.47159100956692 26.9686466604184 30.50009726078268 24.059665069231993 92-93 21.70900420575484 30.441471631048906 31.200393755323507 16.649130407872747 94-95 19.13546417753892 29.19775129936711 31.607915237657487 20.058869285436483 96-97 14.301667747068183 30.363309564499968 34.57009841475632 20.764924273675526 98-99 18.52325574541575 32.240638464666944 27.201942743299718 22.03416304661759 100-101 16.786414493565456 28.430207703760484 30.946828426964636 23.83654937570942 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2759.0 1 1637.0 2 473.0 3 390.0 4 307.0 5 312.0 6 462.5 7 819.5 8 1088.5 9 1015.0 10 718.0 11 451.0 12 385.5 13 388.5 14 360.5 15 364.5 16 410.5 17 428.0 18 467.0 19 540.0 20 604.0 21 695.5 22 826.5 23 934.0 24 1043.5 25 1412.0 26 2278.5 27 3357.5 28 3553.5 29 3648.5 30 4001.0 31 4219.0 32 4280.0 33 4045.5 34 4858.0 35 5651.0 36 5869.0 37 5793.0 38 4144.5 39 2482.5 40 2005.5 41 1736.5 42 1564.0 43 1469.0 44 1405.0 45 1525.5 46 2949.0 47 11186.0 48 27493.0 49 26281.0 50 9676.5 51 2434.5 52 901.0 53 501.5 54 381.0 55 285.5 56 236.0 57 217.5 58 193.5 59 177.5 60 199.5 61 207.5 62 163.5 63 112.0 64 65.5 65 48.5 66 40.0 67 33.0 68 29.0 69 22.0 70 18.0 71 15.5 72 9.0 73 7.0 74 7.0 75 3.5 76 3.5 77 5.0 78 3.5 79 1.0 80 1.0 81 1.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03771161223034724 2 0.025926733408363732 3 0.02298051370286785 4 0.038890100112545595 5 0.08661885934157883 6 0.10429617757455409 7 0.049496491052330754 8 0.08602961540047964 9 0.0724770047551986 10-11 0.07218238278464902 12-13 0.06363834563871097 14-15 0.05686204031607045 16-17 0.04625564937628529 18-19 0.059219016080467146 20-21 0.05155884484617786 22-23 0.02710522129056208 24-25 0.007070927293190108 26-27 0.0032408416760454665 28-29 0.004124707587694229 30-31 0.002651597734946291 32-33 0.005303195469892582 34-35 0.004124707587694229 36-37 0.0032408416760454665 38-39 0.0011784878821983512 40-41 0.005303195469892582 42-43 0.003535463646595054 44-45 0.001767731823297527 46-47 8.838659116487635E-4 48-49 0.0 50-51 8.838659116487635E-4 52-53 0.0032408416760454665 54-55 0.007660171234289284 56-57 0.004124707587694229 58-59 0.002651597734946291 60-61 2.946219705495878E-4 62-63 0.005303195469892582 64-65 8.838659116487635E-4 66-67 0.016793452321326507 68-69 0.04006858799474394 70-71 0.05038035696397952 72-73 0.003830085617144642 74-75 0.0120795007925331 76-77 0.018561184144624033 78-79 0.022096647791219087 80-81 0.0651114554914589 82-83 0.023864379614516616 84-85 0.00677630532264052 86-87 0.025337489467264555 88-89 0.043604051641339 90-91 0.03653312434814889 92-93 0.035649258436500124 94-95 0.006481683352090933 96-97 0.011195634880884337 98-99 0.030640684937157135 100-101 0.06864691913805397 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 169709.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.809739023858487 #Duplication Level Percentage of deduplicated Percentage of total 1 78.75750368105108 16.38923097773247 2 10.015290519877675 4.168311639335569 3 3.5706195492128217 2.2291098291781815 4 1.6111677426662137 1.3411192099417237 5 0.928757503681051 0.966360063402648 6 0.6937365500056631 0.8661885934157882 7 0.4643787518405255 0.6764520443818536 8 0.37376826367652055 0.6222416018007295 9 0.30297881979839164 0.5674419152785062 >10 2.4096726696115076 10.595195304904276 >50 0.3992524634726469 5.771644403066426 >100 0.37943141918677087 15.99031282960833 >500 0.028315777551251555 4.319158088256957 >1k 0.05946313285762827 26.689215068146062 >5k 0.005663155510250311 8.808018431550478 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 8747 5.154116752794489 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6201 3.6539016787559877 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4343 2.5590864361937196 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2997 1.7659640914742294 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2827 1.6657926214873695 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2809 1.6551862305475844 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2635 1.5526577847963277 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2614 1.5402836620332452 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2492 1.4683959012191459 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2489 1.4666281693958483 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2390 1.4082930192270298 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2306 1.358796528174699 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1906 1.1230989517350287 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1838 1.083030363740285 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1818 1.0712454849183013 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1783 1.0506219469798301 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1708 1.006428651397392 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1605 0.9457365254641769 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1530 0.9015432298817387 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1494 0.8803304480021683 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1429 0.842029591830722 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1258 0.741268877902763 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1023 0.6027965517444567 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 972 0.5727451107483987 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 913 0.5379797182235474 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 833 0.4908402029356133 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 799 0.47080590893824137 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 773 0.45548556646966276 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 677 0.3989181481241419 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 662 0.39007948900765427 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 645 0.3800623420089683 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 534 0.3146562645469598 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 522 0.3075853372537697 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 496 0.29226499478519113 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 491 0.2893187750796953 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 479 0.28224784778650513 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 474 0.2793016280810092 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 464 0.2734091886700175 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 456 0.2686952371412241 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 451 0.2657490174357282 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 450 0.26515977349462905 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 413 0.24335774767395954 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 408 0.24041152796846366 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 391 0.23039438096977768 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 387 0.22803740520538096 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 383 0.22568042944098426 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 375 0.22096647791219085 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 357 0.2103600869724057 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 350 0.20623537938471145 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 345 0.20328915967921557 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 343 0.20211067179701725 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 336 0.19798596420932302 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 331 0.19503974450382713 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 320 0.1885580611517362 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 302 0.17795167021195105 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 297 0.17500545050645516 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 290 0.17088074291876093 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 289 0.17029149897766177 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 288 0.1697022550365626 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 285 0.16793452321326507 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 282 0.16616679138996754 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 270 0.15909586409677742 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 265 0.15614964439128154 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 264 0.15556040045018238 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 256 0.15084644892138896 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 248 0.14613249739259557 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 242 0.1425970337460005 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 238 0.1402400579816038 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 235 0.13847232615830626 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 234 0.1378830822172071 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 232 0.13670459433500876 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 231 0.13611535039390957 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 228 0.13434761857061206 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 219 0.12904442310071948 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 212 0.12491971551302525 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 208 0.12256273974862854 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 204 0.12020576398423183 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 196 0.11549181245543842 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 196 0.11549181245543842 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 195 0.11490256851433925 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 193 0.1137240806321409 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 192 0.11313483669104173 No Hit GGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAAT 190 0.11195634880884336 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 189 0.11136710486774419 No Hit GCCTAACGAGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTC 187 0.11018861698554586 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 184 0.10842088516224832 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 183 0.10783164122114915 No Hit TACCACTGCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 179 0.10547466545675244 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 179 0.10547466545675244 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 178 0.10488542151565326 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 177 0.10429617757455409 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 177 0.10429617757455409 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 177 0.10429617757455409 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 176 0.10370693363345493 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 173 0.10193920181015739 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 172 0.10134995786905822 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.946219705495878E-4 0.0 14-15 0.0 0.0 0.0 5.892439410991756E-4 0.0 16-17 0.0 0.0 0.0 5.892439410991756E-4 0.0 18-19 0.0 0.0 0.0 0.0011784878821983512 0.0 20-21 0.0 0.0 0.0 0.0020623537938471146 0.0 22-23 0.0 0.0 0.0 0.005303195469892581 0.0 24-25 0.0 0.0 0.0 0.007660171234289283 0.0 26-27 0.0 0.0 0.0 0.011490256851433924 0.0 28-29 0.0 0.0 0.0 0.023864379614516613 0.0 30-31 0.0 0.0 0.0 0.045077161494086936 0.0 32-33 0.0 0.0 0.0 0.07306624869629778 0.0 34-35 0.0 0.0 0.0 0.1013499578690582 0.0 36-37 0.0 0.0 0.0 0.13994543601105422 0.0 38-39 0.0 0.0 0.0 0.19474512253327755 0.0 40-41 0.0 0.0 0.0 0.23569757643967026 0.0 42-43 0.0 0.0 0.0 0.2846048235509019 0.0 44-45 0.0 0.0 0.0 0.3161293743997078 0.0 46-47 0.0 0.0 0.0 0.37888385412676995 0.0 48-49 0.0 0.0 0.0 0.4189524421215139 0.0 50-51 0.0 0.0 0.0 0.45136085888196853 0.0 52-53 0.0 0.0 0.0 0.5211862659022208 0.0 54-55 0.0 0.0 0.0 0.659069348119428 0.0 56-57 0.0 0.0 0.0 0.8868121313542594 0.0 58-59 0.0 0.0 0.0 1.0874496932985287 0.0 60-61 0.0 0.0 0.0 1.328155843237542 0.0 62-63 0.0 0.0 0.0 1.4651550595431002 0.0 64-65 0.0 0.0 0.0 1.6236616796987784 0.0 66-67 0.0 0.0 0.0 1.9518705548910193 0.0 68-69 0.0 0.0 0.0 2.446540843443777 0.0 70-71 0.0 0.0 0.0 2.8637255537419937 0.0 72-73 0.0 0.0 0.0 3.270009251129875 0.0 74-75 0.0 0.0 0.0 3.7243163297173396 0.0 76-77 0.0 0.0 0.0 4.034553264706056 0.0 78-79 0.0 0.0 0.0 4.425516619625359 0.0 80-81 0.0 0.0 0.0 4.76757272743343 0.0 82-83 0.0 0.0 0.0 5.136734056532064 0.0 84-85 0.0 0.0 0.0 5.810239881208421 0.0 86-87 0.0 0.0 0.0 6.640189972246611 0.0 88-89 0.0 0.0 0.0 7.65575190473104 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGTACT 20 0.002066484 71.28938 4 TATGACC 20 0.002066484 71.28938 4 AATTATC 20 0.002066484 71.28938 5 AGGCATT 95 7.2759576E-12 50.027637 6 CCTTTAG 95 7.2759576E-12 50.027637 1 GGCATTC 95 7.2759576E-12 50.027637 7 TTAGGCA 110 0.0 47.526253 4 CTTTAGG 110 0.0 47.526253 2 GGGTGGC 55 4.929247E-5 43.205685 8 ATGGGGT 190 0.0 42.52349 5 TAGGCAT 125 3.6379788E-12 41.8231 5 CATTCCG 115 5.4569682E-11 41.31499 9 CATGGGG 285 0.0 38.35452 4 GCATTCC 125 1.3278623E-10 38.021004 8 GGTGGCA 50 0.0016098943 38.00979 9 TGGGGTG 185 0.0 35.965813 6 GGGGTGA 135 1.1781594E-8 31.684168 7 CGCAGAA 135 1.1814336E-8 31.674828 9 CATGGGT 140 1.6787453E-8 30.552593 4 GGGTGAG 120 5.3233325E-6 27.723646 8 >>END_MODULE