##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936852_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 169709 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.88177409565786 31.0 31.0 34.0 30.0 34.0 2 31.952424444195653 31.0 31.0 34.0 30.0 34.0 3 32.0449769900241 33.0 31.0 34.0 30.0 34.0 4 35.721299400738914 37.0 35.0 37.0 33.0 37.0 5 35.59812384729154 37.0 35.0 37.0 33.0 37.0 6 35.782816468189665 37.0 35.0 37.0 35.0 37.0 7 35.767484340842266 37.0 35.0 37.0 33.0 37.0 8 35.75849247830109 37.0 35.0 37.0 33.0 37.0 9 37.29562957768887 39.0 37.0 39.0 34.0 39.0 10-11 37.21407821624074 39.0 37.0 39.0 33.5 39.0 12-13 37.17781025166609 39.0 37.0 39.0 33.0 39.0 14-15 38.03942925831865 40.0 37.0 41.0 33.0 41.0 16-17 38.09899003588495 39.5 37.0 41.0 33.5 41.0 18-19 38.344934564460345 40.0 37.5 41.0 34.0 41.0 20-21 38.405688561007366 40.0 38.0 41.0 34.0 41.0 22-23 38.30285960084615 40.0 37.5 41.0 34.0 41.0 24-25 38.086621805561286 40.0 37.0 41.0 33.5 41.0 26-27 37.74154287633537 39.5 36.5 41.0 33.0 41.0 28-29 37.645121944033605 39.0 36.0 41.0 33.0 41.0 30-31 37.60171528911253 39.0 36.0 41.0 33.0 41.0 32-33 37.244268129562954 39.0 35.0 41.0 33.0 41.0 34-35 36.9168547336912 39.0 35.0 41.0 32.0 41.0 36-37 36.62890005833515 38.5 35.0 40.5 31.5 41.0 38-39 36.33190343470293 38.0 35.0 40.0 31.0 41.0 40-41 36.14082930192271 39.0 35.0 40.0 30.5 41.0 42-43 35.73280733490858 38.0 35.0 40.0 30.0 41.0 44-45 35.26017771597263 38.0 35.0 40.0 28.0 41.0 46-47 34.568920917570665 38.0 35.0 40.0 23.5 41.0 48-49 33.881482419907016 38.0 34.0 40.0 18.0 41.0 50-51 33.49502678113713 38.0 33.0 40.0 10.5 41.0 52-53 33.378309341284194 38.0 33.0 40.0 7.5 41.0 54-55 33.06046526701589 38.0 33.0 40.0 2.0 41.0 56-57 32.716653212263346 38.0 32.5 40.0 2.0 41.0 58-59 32.468761232462626 38.0 32.0 40.0 2.0 41.0 60-61 31.849097572904206 36.5 31.0 39.0 2.0 40.5 62-63 31.588333559210177 36.0 31.0 39.0 2.0 40.0 64-65 31.32581359857167 36.0 31.0 39.0 2.0 40.0 66-67 30.79984267186773 35.5 30.5 38.5 2.0 40.0 68-69 30.206750967833173 35.0 29.5 37.0 2.0 39.0 70-71 29.572862959536618 34.5 29.0 37.0 2.0 39.0 72-73 29.052018455120233 34.0 28.5 36.0 2.0 37.5 74-75 28.688257546741774 34.0 29.0 36.0 2.0 37.0 76-77 27.28200331155095 31.5 26.5 34.0 2.0 36.0 78-79 27.941275948830054 33.5 28.0 35.0 2.0 36.0 80-81 27.65024247388176 33.0 26.5 35.0 2.0 36.0 82-83 27.274947115356284 33.0 26.0 35.0 2.0 35.5 84-85 26.829841080909084 33.0 26.0 35.0 2.0 35.0 86-87 26.73175553447372 33.0 26.0 35.0 2.0 35.0 88-89 26.295653147446508 33.0 25.0 35.0 2.0 35.0 90-91 25.71891296277746 32.0 23.5 34.0 2.0 35.0 92-93 25.292924947999225 32.0 20.0 34.0 2.0 35.0 94-95 24.64024889664072 32.0 18.0 34.0 2.0 35.0 96-97 23.806070980325146 31.0 4.5 34.0 2.0 35.0 98-99 23.151320790293973 31.0 2.0 34.0 2.0 35.0 100-101 20.21399572208899 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2161896958612637 1101 2 0.49804576268287803 1101 3 0.44387272952769763 1101 4 0.2425526416749264 1101 5 0.12478138442995856 1101 6 0.13731742251799517 1101 7 0.2653099552238132 1101 8 0.06680718898036275 1101 9 -0.02318356107658559 1101 10-11 0.3689426494092345 1101 12-13 0.4644519578101338 1101 14-15 0.2657494934244369 1101 16-17 0.5678107087898354 1101 18-19 0.22710048799115157 1101 20-21 -0.18961201595514154 1101 22-23 0.2500219844481393 1101 24-25 -0.08732205174074892 1101 26-27 0.02323836402927526 1101 28-29 0.14983744200684157 1101 30-31 0.12501254503549575 1101 32-33 0.6863074457170981 1101 34-35 0.4647443809540448 1101 36-37 0.6380715009144637 1101 38-39 0.38133992827967944 1101 40-41 0.49729212179139637 1101 42-43 0.823886197159247 1101 44-45 0.7402772358602547 1101 46-47 1.1422919315565991 1101 48-49 1.1649316208820366 1101 50-51 0.7753429044406914 1101 52-53 0.5201828159828352 1101 54-55 -0.24240865444417636 1101 56-57 0.5183355058434103 1101 58-59 0.6996338442867938 1101 60-61 0.8306782860922652 1101 62-63 1.0455058483347983 1101 64-65 0.682089916960475 1101 66-67 0.5480866204367238 1101 68-69 0.47815986998081783 1101 70-71 0.8256904899199995 1101 72-73 1.2297485451964185 1101 74-75 1.437797210417937 1101 76-77 1.4328780937453907 1101 78-79 1.332644422326851 1101 80-81 1.6650286700547063 1101 82-83 2.1179578793431837 1101 84-85 2.1514015183339943 1101 86-87 1.7896482312134765 1101 88-89 1.8189918479079061 1101 90-91 1.5937131078265523 1101 92-93 2.1465355172701557 1101 94-95 1.9736143152224521 1101 96-97 1.4374038252209473 1101 98-99 0.947206823625212 1101 100-101 1.04178799096767 1102 1 0.03106396017373214 1102 2 -0.021750920035149335 1102 3 0.1461199205389363 1102 4 0.07140496912276006 1102 5 -0.04589812332820031 1102 6 0.18694289068279346 1102 7 0.23621685731048814 1102 8 0.4763845030520599 1102 9 0.14321558285064384 1102 10-11 0.39780166492555225 1102 12-13 0.21849823924405598 1102 14-15 -0.008595075863958357 1102 16-17 -0.3103661237409341 1102 18-19 0.28685837991196195 1102 20-21 0.6605418096200282 1102 22-23 0.3735840497236893 1102 24-25 0.1471616293721496 1102 26-27 0.4507062613005388 1102 28-29 0.4176611445215528 1102 30-31 0.4090548136283232 1102 32-33 0.39718224507505084 1102 34-35 0.25296669235051183 1102 36-37 0.30988798031521014 1102 38-39 0.04971205241022858 1102 40-41 0.3514587884580678 1102 42-43 0.5613126284059078 1102 44-45 0.9063419763632012 1102 46-47 0.8288556019685771 1102 48-49 0.2722751200794704 1102 50-51 0.557178110433199 1102 52-53 0.6616689048003792 1102 54-55 0.5536386970807072 1102 56-57 0.5406402142436235 1102 58-59 0.4795629507608439 1102 60-61 0.856494230447538 1102 62-63 0.9065946658842847 1102 64-65 0.5664197724981967 1102 66-67 1.0299953951825778 1102 68-69 0.9808350973751452 1102 70-71 0.6700925765973658 1102 72-73 0.7052367741958818 1102 74-75 0.8497822291445303 1102 76-77 1.2129409615891547 1102 78-79 0.26783703869924125 1102 80-81 0.10647463046133865 1102 82-83 0.2645603405523893 1102 84-85 0.7628916199926365 1102 86-87 1.0312212649213421 1102 88-89 1.1183497933332696 1102 90-91 0.8977527011919904 1102 92-93 0.21836537280787738 1102 94-95 0.15205198242416174 1102 96-97 0.3726633222781963 1102 98-99 0.5446519814636304 1102 100-101 1.2314616132255587 1103 1 0.2314488381922324 1103 2 0.15616204787099264 1103 3 -0.05060240859384635 1103 4 0.31269865588171797 1103 5 0.07729388114135105 1103 6 -0.03901775243596006 1103 7 0.2032539173390404 1103 8 0.135736418130584 1103 9 -0.02923460027680136 1103 10-11 0.028489479190866973 1103 12-13 0.20940394412952656 1103 14-15 0.018051519207126887 1103 16-17 0.22327966432784763 1103 18-19 0.4308462128004109 1103 20-21 0.3804188969625102 1103 22-23 0.47014497839710145 1103 24-25 0.6077581903008209 1103 26-27 0.19929400728104696 1103 28-29 0.38919879428255655 1103 30-31 0.26770525747958374 1103 32-33 0.23987887428852872 1103 34-35 0.39040539121450024 1103 36-37 0.26353190607099464 1103 38-39 0.21399909691951535 1103 40-41 0.006154921054751128 1103 42-43 -0.5977133818988918 1103 44-45 -0.7786322616404036 1103 46-47 -0.896267000303439 1103 48-49 -0.7917574083208052 1103 50-51 -0.9226313128705428 1103 52-53 -0.3920277864902033 1103 54-55 -0.43464425033998566 1103 56-57 -0.37552466253332994 1103 58-59 -0.8076833090938962 1103 60-61 -0.4509581253783992 1103 62-63 0.09111884491464295 1103 64-65 0.035039798570579705 1103 66-67 0.4661180595316985 1103 68-69 0.20671814411920053 1103 70-71 0.22096475984896458 1103 72-73 0.12824565122114606 1103 74-75 -0.1321843733758037 1103 76-77 0.17352200324289058 1103 78-79 0.0760343197222717 1103 80-81 0.4774628978894846 1103 82-83 0.2051059982592598 1103 84-85 0.4616159355926115 1103 86-87 -0.2573130947533713 1103 88-89 0.1536406245456341 1103 90-91 0.45506538354347015 1103 92-93 0.5184771115874405 1103 94-95 1.107263354948838 1103 96-97 0.4118184529504916 1103 98-99 0.5170897491711237 1103 100-101 0.8480889643957141 1104 1 0.2838729489377734 1104 2 0.36588952940305575 1104 3 0.09555812278612308 1104 4 0.20061845226882724 1104 5 0.15634906768303125 1104 6 0.29368446371650236 1104 7 0.17441910450149578 1104 8 -0.0067615643636713685 1104 9 0.19939535813154663 1104 10-11 0.24049829413903723 1104 12-13 0.3926555426148397 1104 14-15 0.14870829492256377 1104 16-17 0.4087349998545733 1104 18-19 -0.036175327953209546 1104 20-21 -0.0585593139754792 1104 22-23 0.29212337556637635 1104 24-25 0.12468971925979133 1104 26-27 0.049020868042113364 1104 28-29 0.31653754901594056 1104 30-31 -0.1836418155851547 1104 32-33 -0.16180651896989673 1104 34-35 0.19116893954151948 1104 36-37 0.34359584548374755 1104 38-39 0.4401614906124749 1104 40-41 0.5930318221659334 1104 42-43 0.465807010428378 1104 44-45 0.3052183808575748 1104 46-47 0.2754848154517262 1104 48-49 0.5559829852480078 1104 50-51 0.860548896950057 1104 52-53 0.6195340733397074 1104 54-55 0.7586948768559836 1104 56-57 1.1305278546930637 1104 58-59 0.9767539619967991 1104 60-61 1.0109886124700083 1104 62-63 0.6200778119517025 1104 64-65 0.9765321320487566 1104 66-67 0.3670740468679732 1104 68-69 0.42465532209424595 1104 70-71 0.6391937001928731 1104 72-73 0.2557985719486915 1104 74-75 0.7430406561108214 1104 76-77 0.9179971413644381 1104 78-79 1.295926926339689 1104 80-81 0.8761375518096557 1104 82-83 0.854447981001826 1104 84-85 0.8527792604420732 1104 86-87 0.6070639615505549 1104 88-89 0.6267767596254039 1104 90-91 0.24887629669760614 1104 92-93 0.20712941775169824 1104 94-95 0.9498047914129266 1104 96-97 0.7771577043006701 1104 98-99 0.5558879365198095 1104 100-101 0.7314616132255622 1105 1 -0.3228353816253531 1105 2 0.10483640793506765 1105 3 -0.24547638289833884 1105 4 0.0357690406605613 1105 5 0.10857963745898047 1105 6 0.23840223514127246 1105 7 0.26520429165660886 1105 8 0.18036030253347235 1105 9 0.06416498781042179 1105 10-11 -0.01026628268432006 1105 12-13 0.13825091526293676 1105 14-15 0.1838954500319332 1105 16-17 -0.16107418836429588 1105 18-19 0.019489189033699006 1105 20-21 0.0783331798746687 1105 22-23 0.19646816647124155 1105 24-25 -0.2528616403572599 1105 26-27 -0.3213833604001408 1105 28-29 0.21917035268590013 1105 30-31 0.3277104881180506 1105 32-33 0.15408005772048483 1105 34-35 0.22557489659224927 1105 36-37 0.00912672773891643 1105 38-39 -0.1941214692971016 1105 40-41 0.03193216123913345 1105 42-43 0.43617134790594037 1105 44-45 -0.014125412773161372 1105 46-47 0.17348029447572344 1105 48-49 0.6555275634010513 1105 50-51 1.1964474409298376 1105 52-53 1.2957357886511929 1105 54-55 1.3946898240004586 1105 56-57 0.8481995520536145 1105 58-59 1.0681707560929325 1105 60-61 1.1076577169178563 1105 62-63 1.4853128362187107 1105 64-65 1.1021554283721393 1105 66-67 1.075471094936585 1105 68-69 1.0124653081324126 1105 70-71 0.9297162718303973 1105 72-73 1.3638532225703237 1105 74-75 1.4397695970057072 1105 76-77 1.2833817567490513 1105 78-79 1.1622090884299752 1105 80-81 1.2547194280146918 1105 82-83 1.130144410760483 1105 84-85 0.8758661562601695 1105 86-87 0.9375772252963159 1105 88-89 1.3037762942308184 1105 90-91 0.4266237868910778 1105 92-93 1.2008465908120023 1105 94-95 1.5267278683360033 1105 96-97 0.9598749438459109 1105 98-99 0.4261093313738762 1105 100-101 0.28868852632974296 1106 1 0.07774172407996005 1106 2 0.11356604486982746 1106 3 0.024119643107944455 1106 4 0.09859320604882527 1106 5 0.13359972658162889 1106 6 0.02311989165379913 1106 7 -0.15378175553456686 1106 8 0.07209819427136921 1106 9 0.17699280576644583 1106 10-11 0.2116454712787217 1106 12-13 0.2809202117783869 1106 14-15 0.2780951724367853 1106 16-17 0.25046062060641106 1106 18-19 0.2210378777618729 1106 20-21 0.4480643508878899 1106 22-23 0.31979711672326516 1106 24-25 0.1124133979947004 1106 26-27 0.5506854539763637 1106 28-29 0.688087001089734 1106 30-31 0.3840513457409642 1106 32-33 -0.04715816274850937 1106 34-35 0.026583005292664552 1106 36-37 0.3772690317786527 1106 38-39 0.6160180587911483 1106 40-41 0.6076316279642384 1106 42-43 1.1364964264361461 1106 44-45 0.6474641847138685 1106 46-47 0.4192981043961055 1106 48-49 0.0015718325222451313 1106 50-51 0.8370712995024192 1106 52-53 0.6411043327376831 1106 54-55 0.942666301464115 1106 56-57 0.5585691919104292 1106 58-59 0.74471761853723 1106 60-61 0.5105724659864421 1106 62-63 0.5580719859009378 1106 64-65 0.4879761603467223 1106 66-67 0.3242109590610589 1106 68-69 0.3899764484640613 1106 70-71 0.4245661512956609 1106 72-73 0.807019268300472 1106 74-75 0.22174782770188628 1106 76-77 0.3871329438335742 1106 78-79 0.9179618826443097 1106 80-81 0.952396394922431 1106 82-83 1.0879755160274875 1106 84-85 1.1939500192158299 1106 86-87 1.0890309472628594 1106 88-89 1.4883040172200772 1106 90-91 0.7691981166448976 1106 92-93 0.8714032421378839 1106 94-95 1.2769652852400846 1106 96-97 1.4140352185190075 1106 98-99 2.011360262778652 1106 100-101 2.7710648869112298 1107 1 0.2693386973973624 1107 2 0.41985835868902655 1107 3 0.27935660049544 1107 4 0.14128536056893637 1107 5 0.2447506624637512 1107 6 0.12387656230294652 1107 7 0.3119689413989235 1107 8 0.2185737020371974 1107 9 0.4354953943765594 1107 10-11 0.3374718352771282 1107 12-13 0.22034673507587854 1107 14-15 0.5033839020265845 1107 16-17 0.29271470264544064 1107 18-19 0.21175870611710934 1107 20-21 0.1350615631539256 1107 22-23 0.009104165707739753 1107 24-25 0.10422940755265131 1107 26-27 0.17894112900622616 1107 28-29 0.5348412822526214 1107 30-31 0.16161008727819848 1107 32-33 0.3882659710627223 1107 34-35 -0.1839307342533374 1107 36-37 -0.31488548833284824 1107 38-39 -0.5522814234868392 1107 40-41 -0.6324121792838682 1107 42-43 -0.3902459072954301 1107 44-45 0.2171973587481162 1107 46-47 -0.22558441252942885 1107 48-49 -0.482418609532715 1107 50-51 -0.5708936546990984 1107 52-53 -0.4887841579034955 1107 54-55 -0.11147547472067032 1107 56-57 -0.3056084265682628 1107 58-59 -0.3434345120880167 1107 60-61 -0.4662495172871495 1107 62-63 -0.49162932831650963 1107 64-65 -0.3526451060809954 1107 66-67 -0.3497436406999874 1107 68-69 -0.6790597920775525 1107 70-71 -0.6100813994809222 1107 72-73 -0.622690010871171 1107 74-75 -0.5127404239906674 1107 76-77 -0.5932931812162074 1107 78-79 -0.7064108772123205 1107 80-81 -1.0933622669652614 1107 82-83 -0.7612279885523598 1107 84-85 -0.9237520914970325 1107 86-87 -0.5651904784639434 1107 88-89 -0.47179156223034013 1107 90-91 -0.8476079367202978 1107 92-93 -0.8490866025455084 1107 94-95 -0.9276145634257809 1107 96-97 0.08986158251741116 1107 98-99 0.15269837508450834 1107 100-101 0.4357204026420156 1108 1 0.10925569419041992 1108 2 0.11584024889842226 1108 3 0.13685518566804689 1108 4 0.1265334269715055 1108 5 -0.06360625132609954 1108 6 0.208954849418582 1108 7 -0.13851209991403124 1108 8 0.011669344368833379 1108 9 0.3352124288983518 1108 10-11 0.37912435366988717 1108 12-13 0.269224305345638 1108 14-15 0.44411206652385005 1108 16-17 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>>Per sequence quality scores pass #Quality Count 4 3.0 5 12.0 6 32.0 7 48.0 8 75.0 9 174.0 10 335.0 11 575.0 12 923.0 13 1262.0 14 1518.0 15 2141.0 16 3029.0 17 3957.0 18 3034.0 19 1570.0 20 1273.0 21 1238.0 22 1228.0 23 1462.0 24 1540.0 25 1889.0 26 2206.0 27 2577.0 28 3160.0 29 3863.0 30 4854.0 31 6065.0 32 7403.0 33 9501.0 34 13200.0 35 18386.0 36 25642.0 37 32105.0 38 13175.0 39 254.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.98597401066683 21.231694021274716 16.546542122167544 14.235789845890915 2 14.495580436063642 27.642309958750737 33.97053624042427 23.891573364761342 3 28.2348019256492 24.355219817452227 35.58620933480252 11.82376892209606 4 11.018861698554584 19.760884808701952 43.376014236133614 25.844239256609846 5 8.172368684666424 42.78752666375968 36.695815115552776 12.344289536021119 6 22.131413183743938 40.55589273403296 17.47756453694265 19.83512954528045 7 19.602378188546275 40.8186955317632 22.276367193254334 17.302559086436194 8 23.742406118709084 25.329829296030265 21.871556605719203 29.056207979541448 9 19.57880843090231 24.18433907453347 28.802833084868805 27.43401940969542 10-11 23.97309512164941 25.275029609508042 27.77637013947404 22.975505129368507 12-13 30.600856219376016 26.894009705391 26.252581769539685 16.2525523056933 14-15 23.025906796814464 28.623578043010056 34.451649779469605 13.898865380705875 16-17 15.314155407197024 31.317726225480087 33.827021548650926 19.541096818671964 18-19 14.796548198823276 34.46969362171017 34.79318949846354 15.940568681003015 20-21 18.93710612145086 26.27389051097041 32.509656377333805 22.27934699024492 22-23 16.563645363157462 27.015249902773032 35.46250574522999 20.958598988839523 24-25 22.76269463635962 27.413932796134528 33.024468570923496 16.798903996582354 26-27 20.248603483630706 23.1506469936597 41.52514907959554 15.075600443114054 28-29 15.134480594666739 33.66007006131102 35.82731566185547 15.378133682166775 30-31 17.714735223235067 27.11229221785527 34.620438515340965 20.552534043568695 32-33 17.959271458791225 27.995274263592385 37.12089517939532 16.924559098221074 34-35 21.513001667560353 26.484158176643547 34.387097914665695 17.615742241130405 36-37 19.190654370819953 24.84428861848502 39.63672254795085 16.328334462744174 38-39 17.59071288616509 28.99306118239809 37.35735647254674 16.058869458890086 40-41 17.383003547311233 30.480360152263327 35.08302593897682 17.053610361448623 42-43 15.900158506620155 30.696073867620456 31.45708241755004 21.946685208209345 44-45 20.048553986848 25.772503358710253 32.61248733118062 21.56645532326113 46-47 23.22541298756397 29.971981368051686 32.52135279022559 14.28125285415875 48-49 20.417891803027537 28.072465219876374 33.64082046326359 17.8688225138325 50-51 13.771341867625178 30.287111647982556 38.17597925842994 17.765567225962318 52-53 19.15643340325736 33.885556367407546 28.598239328729345 18.359770900605746 54-55 16.347100035943882 27.34268659882505 32.49091091220854 23.819302453022527 56-57 21.32456926014095 27.985127395290732 29.93317934334268 20.757124001225634 58-59 26.279395907111585 27.322357682857124 28.608971828247178 17.789274581784113 60-61 19.749034350886394 29.001882684313024 35.816964193642484 15.432118771158102 62-63 17.352644821429624 29.293673287804417 34.51142838623762 18.84225350452834 64-65 16.54714835394705 34.481966189182664 31.36604422865022 17.60484122822007 66-67 19.360198928754517 29.179654585201725 28.11076607604782 23.349380409995934 68-69 16.630526371612582 28.79369980378177 31.82241366103153 22.75336016357412 70-71 22.57979930114845 29.705021006794063 28.92396895826336 18.791210733794127 72-73 20.498504780283724 24.73225991779974 36.71640911568434 18.052826186232192 74-75 16.43724624785655 35.24333688856414 30.777593796220454 17.541823067358855 76-77 18.253893429340813 28.92068187308864 29.890872022108432 22.934552675462115 78-79 18.38776015273293 30.135291206071606 31.614302213213286 19.86264642798218 80-81 21.284224730488994 28.045054520920065 30.342564030040275 20.328156718550666 82-83 18.109234041800963 28.001166703003378 34.612483722136126 19.27711553305953 84-85 17.652067588868757 30.219649091792988 32.00394808562043 20.12433523371783 86-87 17.060825243433555 31.686578377502467 30.466243389361107 20.786352989702873 88-89 16.905222095202177 31.154317568443506 27.590717846578116 24.349742489776197 90-91 18.69031940626556 25.203072381272264 31.32868410275448 24.7779241097077 92-93 21.340058570847745 29.05679722348255 32.03247912603339 17.57066507963632 94-95 19.172819355484975 27.606373262466928 32.564271782875394 20.656535599172702 96-97 13.9212647606477 29.074351238598062 35.75965776510241 21.244726235651825 98-99 18.03220246600769 31.276166345034838 27.996994829338718 22.694636359618755 100-101 15.934898027730801 27.519254226501594 31.5999127888134 24.945934956954208 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2970.0 1 1841.5 2 622.0 3 491.5 4 373.0 5 308.5 6 415.0 7 703.0 8 918.0 9 865.5 10 663.0 11 478.5 12 392.0 13 364.0 14 369.0 15 362.5 16 367.0 17 419.0 18 477.5 19 521.0 20 614.5 21 743.0 22 829.0 23 911.5 24 1065.5 25 1405.0 26 2190.5 27 3308.0 28 3656.5 29 3697.0 30 3917.0 31 4082.0 32 4137.0 33 3948.5 34 4777.5 35 5714.5 36 5963.5 37 5731.5 38 4122.5 39 2570.0 40 2075.0 41 1785.0 42 1532.0 43 1414.5 44 1373.0 45 1472.0 46 2682.0 47 11162.5 48 27872.5 49 26609.5 50 9990.0 51 2574.5 52 852.0 53 433.0 54 324.5 55 245.0 56 199.0 57 175.0 58 164.5 59 158.0 60 172.0 61 175.5 62 140.5 63 92.0 64 49.0 65 32.5 66 23.5 67 20.0 68 19.5 69 16.0 70 14.5 71 14.0 72 8.0 73 1.5 74 2.5 75 4.0 76 2.0 77 2.5 78 2.5 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.014141854586380216 2 0.005303195469892582 3 0.0 4 0.0 5 0.001767731823297527 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005597817440442169 14-15 0.002651597734946291 16-17 0.0 18-19 2.946219705495878E-4 20-21 0.0014731098527479391 22-23 0.001767731823297527 24-25 8.838659116487635E-4 26-27 5.892439410991756E-4 28-29 2.946219705495878E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0023569757643967025 38-39 0.00677630532264052 40-41 0.001767731823297527 42-43 0.0 44-45 5.892439410991756E-4 46-47 2.946219705495878E-4 48-49 0.0 50-51 8.838659116487635E-4 52-53 5.892439410991756E-4 54-55 0.0 56-57 5.892439410991756E-4 58-59 0.0 60-61 0.002651597734946291 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0011784878821983512 72-73 8.838659116487635E-4 74-75 0.003535463646595054 76-77 0.0 78-79 5.892439410991756E-4 80-81 0.0020623537938471146 82-83 0.0 84-85 0.003830085617144642 86-87 8.838659116487635E-4 88-89 0.004124707587694229 90-91 0.0020623537938471146 92-93 0.0 94-95 0.0 96-97 5.892439410991756E-4 98-99 8.838659116487635E-4 100-101 0.003535463646595054 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 169709.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 20.19338986146875 #Duplication Level Percentage of deduplicated Percentage of total 1 77.99824919754887 15.750490545580965 2 10.148818208345492 4.098780854285867 3 3.691275167785235 2.2361807564713714 4 1.914210679894952 1.5461761014442368 5 1.0271374379924132 1.0370693363345491 6 0.7324190253866355 0.8874013752953585 7 0.4727166618033265 0.6682026292064652 8 0.3559964983950978 0.5751020865127955 9 0.28304639626495476 0.5144099605795803 >10 2.5007295010213015 10.720704264358401 >50 0.4201925882696236 6.018537614386981 >100 0.3559964983950978 15.172442239362674 >500 0.02918004085205719 4.085817487581684 >1k 0.06419608987452582 27.886558756459586 >5k 0.005836008170411439 8.802125992139485 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 9617 5.666758981550772 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 5321 3.135367010588714 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4548 2.679881444119051 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3217 1.8955977585160482 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 3181 1.8743849766364777 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2903 1.710575161010907 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2705 1.59390486067327 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 2699 1.5903693970266752 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 2678 1.5779952742635925 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 2655 1.5644426636183113 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2363 1.392383432817352 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2265 1.3346375265896329 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2195 1.2933904507126905 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1820 1.0724239728004996 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1739 1.0246952135714666 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1726 1.0170350423371772 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1658 0.9769664543424332 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1623 0.9563429164039621 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1601 0.9433795496997802 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1405 0.8278877372443418 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1179 0.6947186065559281 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1071 0.6310802609172171 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1049 0.6181168942130353 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1046 0.6163491623897377 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 906 0.5338550106358532 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 900 0.5303195469892581 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 869 0.5120529848151837 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 799 0.47080590893824137 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 703 0.41423849059272044 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 583 0.3435292176608194 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 562 0.3311550948977367 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 550 0.3240841676045466 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 545 0.3211379478990507 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 517 0.3046391175482738 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 499 0.2940327266084886 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 479 0.28224784778650513 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 469 0.2763554083755134 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 459 0.27046296896452166 No Hit GCTTTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 459 0.27046296896452166 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 457 0.2692844810823233 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 436 0.2569103583192406 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 430 0.2533748946726455 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 429 0.25278565073154635 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 426 0.2510179189082488 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 418 0.24630396737945542 No Hit TTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGA 382 0.22509118549988508 No Hit ATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCA 378 0.22273420973548838 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 353 0.208003111208009 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 346 0.20387840362031479 No Hit TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA 343 0.20211067179701725 No Hit CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA 335 0.19739672026822383 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 327 0.19268276873943044 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 315 0.18561184144624032 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 310 0.18266562174074447 No Hit GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG 309 0.18207637779964528 No Hit GTACATGGGGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCCTAACGAGCT 302 0.17795167021195105 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 275 0.1620420838022733 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 273 0.16086359592007496 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 272 0.16027435197897577 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 270 0.15909586409677742 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 267 0.1573281322734799 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 262 0.154381912567984 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 257 0.15143569286248812 No Hit CAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAAC 254 0.1496679610391906 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 249 0.14672174133369473 No Hit CGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTT 246 0.1449540095103972 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 240 0.14141854586380215 No Hit GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT 235 0.13847232615830626 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 234 0.1378830822172071 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 234 0.1378830822172071 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 230 0.1355261064528104 No Hit CCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATT 229 0.13493686251171122 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 228 0.13434761857061206 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 223 0.13140139886511618 No Hit CTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGC 221 0.1302229109829178 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 219 0.12904442310071948 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 218 0.1284551791596203 No Hit GAGTAAGCAGTGGTATCAACGCAGAGCAAGCAGTGGTATCAACGCAGAGT 216 0.12727669127742194 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 214 0.1260982033952236 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 212 0.12491971551302525 No Hit CTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCA 210 0.12374122763082689 No Hit TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC 208 0.12256273974862854 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 208 0.12256273974862854 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 208 0.12256273974862854 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 207 0.12197349580752936 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 205 0.120795007925331 No Hit AGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTG 204 0.12020576398423183 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 200 0.11784878821983512 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 199 0.11725954427873596 No Hit GTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGATTTTGTTGTTTTT 192 0.11313483669104173 No Hit ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC 190 0.11195634880884336 No Hit AGCTTGGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAA 189 0.11136710486774419 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 189 0.11136710486774419 No Hit ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT 185 0.1090101291033475 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 182 0.10724239728004997 No Hit CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG 178 0.10488542151565326 No Hit GCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAG 178 0.10488542151565326 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 174 0.10252844575125655 No Hit GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT 174 0.10252844575125655 No Hit TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG 173 0.10193920181015739 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGC 171 0.10076071392795904 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 170 0.10017146998685986 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 2.946219705495878E-4 0.0 14-15 0.0 0.0 0.0 5.892439410991756E-4 0.0 16-17 0.0 0.0 0.0 8.838659116487634E-4 0.0 18-19 0.0 0.0 0.0 0.001767731823297527 0.0 20-21 0.0 0.0 0.0 0.0026515977349462904 0.0 22-23 0.0 0.0 0.0 0.0058924394109917564 0.0 24-25 0.0 0.0 0.0 0.007954793204838871 0.0 26-27 0.0 0.0 0.0 0.012079500792533102 0.0 28-29 0.0 0.0 0.0 0.02445362355561579 0.0 30-31 0.0 0.0 0.0 0.04625564937628529 0.0 32-33 0.0 0.0 0.0 0.07630709037234325 0.0 34-35 0.0 0.0 0.0 0.10576928742730203 0.0 36-37 0.0 0.0 0.0 0.14377552162819884 0.0 38-39 0.0 0.0 0.0 0.19945907406207097 0.0 40-41 0.0 0.0 0.0 0.24159001585066203 0.0 42-43 0.0 0.0 0.0 0.29462197054958783 0.0 44-45 0.0 0.0 0.0 0.32614652139839373 0.0 46-47 0.0 0.0 0.0 0.3927310867426006 0.0 48-49 0.0 0.0 0.0 0.4336835406489933 0.0 50-51 0.0 0.0 0.0 0.46550271346834876 0.0 52-53 0.0 0.0 0.0 0.5359173644297002 0.0 54-55 0.0 0.0 0.0 0.676157422411304 0.0 56-57 0.0 0.0 0.0 0.9059625594399826 0.0 58-59 0.0 0.0 0.0 1.1077786092664503 0.0 60-61 0.0 0.0 0.0 1.3520202228520586 0.0 62-63 0.0 0.0 0.0 1.492554902804212 0.0 64-65 0.0 0.0 0.0 1.651945388871539 0.0 66-67 0.0 0.0 0.0 1.9801542640637797 0.0 68-69 0.0 0.0 0.0 2.4780653942925834 0.0 70-71 0.0 0.0 0.0 2.897017836414097 0.0 72-73 0.0 0.0 0.0 3.3068369974485736 0.0 74-75 0.0 0.0 0.0 3.7617333199771377 0.0 76-77 0.0 0.0 0.0 4.073148742848051 0.0 78-79 0.0 0.0 0.0 4.465585207620103 0.0 80-81 0.0 0.0 0.0 4.809409047251471 0.0 82-83 0.0 0.0 0.0 5.177391888467907 0.0 84-85 0.0 0.0 0.0 5.8562009086141575 0.0 86-87 0.0 0.0 0.0 6.69057032921059 0.0 88-89 0.0 0.0 0.0 7.711140835194362 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGGGG 30 1.2987625E-4 63.32626 4 TTAGGCA 150 0.0 44.32838 4 AGGCATT 155 0.0 42.91108 6 CCTTTAG 155 0.0 42.898434 1 TAGGCAT 160 0.0 41.55786 5 CATTCCG 170 0.0 39.124805 9 GGCATTC 170 0.0 39.124805 7 CTTTAGG 185 0.0 38.509216 2 ACCTGGG 50 0.0016128209 37.995758 3 TAGTTCT 50 0.0016128209 37.995758 5 GCATTCC 185 0.0 35.952526 8 GATGTGG 55 0.0025711488 34.551777 9 GGATGTG 55 0.0025711488 34.551777 8 TACCTGG 55 0.0025748692 34.5416 2 GCTTTGC 195 0.0 34.09876 1 CTTTGCG 210 0.0 31.66313 2 CATGGGT 130 3.0141018E-7 29.227505 4 GGTGAGA 165 2.6084308E-9 28.793148 9 ATGGGAT 135 4.1935164E-7 28.145008 5 GGGTGAG 170 3.587047E-9 27.946291 8 >>END_MODULE