FastQCFastQC Report
Thu 26 May 2016
SRR936851_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936851_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763301
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82190.2974341195548368No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56930.2060217109898632No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35930.130025646862213No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44450.049.9446641
GTATCAA80800.042.7138941
TATCAAC104550.032.6931462
ATCAACG105200.032.1713183
TCAACGC105350.032.170634
CAACGCA107850.031.3339145
AACGCAG111000.030.5715056
ACGCAGA126600.026.616217
CGCAGAG127750.025.8571838
GTACATG105200.025.0344471
TACATGG105650.024.163322
ACATGGG105300.023.2478853
GCAGAGT140050.022.5685549
CATGGGG63100.020.8667584
AGAGTAC124050.020.05515910-11
GAGTACT72150.019.65942812-13
CAGAGTA134050.019.23264910-11
GTACTTT75050.018.64650714-15
AGTACTT77250.018.17698712-13
GTATAGG7950.017.9389931
Produced by FastQC (version 0.11.5)