FastQCFastQC Report
Thu 26 May 2016
SRR936851_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936851_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2763301
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84350.3052508575793951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64830.2346107065426459No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42360.15329491792606015No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49950.045.4644281
GTATCAA90300.040.7749331
ATCAACG114650.031.8593793
TCAACGC114950.031.6511214
CAACGCA117300.031.0575085
TATCAAC117650.030.9673562
AACGCAG121850.030.0531676
ACGCAGA140200.025.814787
CGCAGAG142100.025.2690628
GTACATG99550.023.193931
GCAGAGT153650.022.8749739
TACATGG103750.022.1593992
GAGTACT82800.022.08471712-13
ACATGGG102900.021.2799473
GTACTTT86950.020.84002314-15
AGAGTAC137200.020.63258410-11
GTGGTAT30900.020.449021
AGTACTT88500.019.93778812-13
TGGTATC30050.019.9171312
CAGAGTA149150.018.61327210-11
Produced by FastQC (version 0.11.5)