##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936851_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2763301 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06395828756983 31.0 31.0 34.0 30.0 34.0 2 32.065613192337715 33.0 31.0 34.0 30.0 34.0 3 32.254970775894485 33.0 31.0 34.0 30.0 34.0 4 35.99874823625801 37.0 35.0 37.0 35.0 37.0 5 35.91581988353784 37.0 35.0 37.0 35.0 37.0 6 35.952698602142874 37.0 35.0 37.0 35.0 37.0 7 35.8900007635795 37.0 35.0 37.0 35.0 37.0 8 35.864176939102904 37.0 35.0 37.0 35.0 37.0 9 37.541938427988846 39.0 37.0 39.0 35.0 39.0 10-11 37.46182952924781 39.0 37.0 39.0 34.5 39.0 12-13 37.35833827006178 39.0 37.0 39.0 34.0 39.0 14-15 38.566280872043976 40.0 38.0 41.0 34.0 41.0 16-17 38.47218308827016 40.0 38.0 41.0 34.0 41.0 18-19 38.50826728611903 40.0 38.0 41.0 34.0 41.0 20-21 38.59032204598775 40.0 38.0 41.0 34.0 41.0 22-23 38.52384032720286 40.0 38.0 41.0 34.0 41.0 24-25 38.42648430265107 40.0 38.0 41.0 34.0 41.0 26-27 38.27500207179746 40.0 38.0 41.0 34.0 41.0 28-29 38.098028227833304 40.0 38.0 41.0 33.0 41.0 30-31 37.990482035797044 40.0 37.0 41.0 33.0 41.0 32-33 38.0084250684236 40.0 37.0 41.0 33.0 41.0 34-35 37.94150800075707 40.0 37.0 41.0 33.0 41.0 36-37 37.800670285285605 40.0 37.0 41.0 33.0 41.0 38-39 37.6537393139582 40.0 37.0 41.0 33.0 41.0 40-41 37.48646799606702 40.0 37.0 41.0 32.5 41.0 42-43 37.2912746385573 40.0 36.5 41.0 32.0 41.0 44-45 36.997992075419944 39.0 36.0 41.0 31.0 41.0 46-47 36.77783835347651 39.0 36.0 41.0 31.0 41.0 48-49 36.502759380899874 39.0 36.0 41.0 30.0 41.0 50-51 36.257841074859385 39.0 35.0 40.0 30.0 41.0 52-53 35.99746263617318 39.0 35.0 40.0 29.0 41.0 54-55 35.756756864344496 38.5 35.0 40.0 28.5 41.0 56-57 35.484973587748854 38.0 34.5 40.0 28.0 41.0 58-59 35.141566372972036 38.0 34.0 40.0 27.5 41.0 60-61 34.81443389627116 38.0 34.0 40.0 27.0 41.0 62-63 34.432816946109014 37.0 33.5 40.0 26.0 41.0 64-65 34.02442567783966 36.5 33.0 39.0 26.0 40.5 66-67 33.62962015357719 36.0 33.0 39.0 26.0 40.0 68-69 33.18797680744878 35.5 32.0 38.5 25.0 40.0 70-71 32.71257275266068 35.0 32.0 38.0 24.0 40.0 72-73 32.3522535185273 35.0 31.0 37.5 23.0 39.5 74-75 31.898922339622068 35.0 31.0 37.0 22.0 39.0 76-77 30.51202764374927 33.5 29.5 35.5 20.5 37.5 78-79 31.250490807914158 34.0 31.0 36.0 21.5 38.0 80-81 31.080324039979722 34.0 31.0 35.5 20.5 37.0 82-83 30.732845245595755 34.0 31.0 35.0 20.0 37.0 84-85 30.347049235678632 34.0 30.0 35.0 18.0 36.5 86-87 29.971401595410704 34.0 30.0 35.0 17.5 36.0 88-89 29.602041905677304 34.0 29.5 35.0 11.5 36.0 90-91 29.161857322094118 34.0 29.0 35.0 7.0 35.0 92-93 28.6685789930232 34.0 29.0 35.0 2.0 35.0 94-95 28.156564739056655 33.0 28.5 35.0 2.0 35.0 96-97 27.487998592987154 33.0 27.0 35.0 2.0 35.0 98-99 26.675158442746557 33.0 25.5 34.0 2.0 35.0 100-101 23.483234363538394 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3086154242240511 1101 2 0.19153462820212752 1101 3 0.20920007758275005 1101 4 0.19400052290426117 1101 5 0.23961760748965588 1101 6 0.21861391322252643 1101 7 0.26124806621535157 1101 8 0.234245723797045 1101 9 0.24522078110486234 1101 10-11 0.30219743832066825 1101 12-13 0.3410087793179244 1101 14-15 0.4513769949994 1101 16-17 0.5801059535617981 1101 18-19 0.4417672841189031 1101 20-21 0.39193363897084055 1101 22-23 0.41688509757742764 1101 24-25 0.3547590160700693 1101 26-27 0.35952425389963594 1101 28-29 0.44163979612361715 1101 30-31 0.46301833160706707 1101 32-33 0.4826928791025935 1101 34-35 0.44540364178332936 1101 36-37 0.41024444372261115 1101 38-39 0.4353232139080703 1101 40-41 0.34099649988903025 1101 42-43 0.43092643942580366 1101 44-45 0.6362150813681495 1101 46-47 0.4824362055526734 1101 48-49 0.41891741714231756 1101 50-51 0.3385555757830119 1101 52-53 0.17863534961524863 1101 54-55 0.13321391567863117 1101 56-57 0.20475911638481392 1101 58-59 0.027458973674363563 1101 60-61 0.2244062917567149 1101 62-63 0.2661909014746371 1101 64-65 0.18135480525707948 1101 66-67 0.2477528561586837 1101 68-69 0.07754020131722683 1101 70-71 -0.01559098557297034 1101 72-73 0.20610451009094533 1101 74-75 0.055816576667997 1101 76-77 0.10426072159773625 1101 78-79 0.2242019239740678 1101 80-81 0.48386360582378174 1101 82-83 0.5399991534233521 1101 84-85 0.45994698988414484 1101 86-87 0.30176262448215496 1101 88-89 0.42396471583017714 1101 90-91 0.6144220979062176 1101 92-93 0.6232010503240488 1101 94-95 0.6564469295310751 1101 96-97 0.8046300631146472 1101 98-99 0.9378320719156044 1101 100-101 1.068339553523959 1102 1 0.3467882658211252 1102 2 0.1968774327215499 1102 3 0.2104140492233455 1102 4 0.21854396962367417 1102 5 0.26052713970870656 1102 6 0.19660155367915166 1102 7 0.2594408325543114 1102 8 0.26569058021532754 1102 9 0.44939153619202443 1102 10-11 0.3684327427773155 1102 12-13 0.35594716622618705 1102 14-15 0.46933867962722076 1102 16-17 0.5174409910259214 1102 18-19 0.4657548246318086 1102 20-21 0.4128668032039897 1102 22-23 0.4255241988125462 1102 24-25 0.39980508126586756 1102 26-27 0.4244956763942582 1102 28-29 0.42438953358670517 1102 30-31 0.434359468100169 1102 32-33 0.5396364941016358 1102 34-35 0.45476130394695247 1102 36-37 0.48249624972543614 1102 38-39 0.4768967269497395 1102 40-41 0.683932472393181 1102 42-43 0.559176949114196 1102 44-45 0.6744057085174973 1102 46-47 0.7741133548228163 1102 48-49 0.6218759852982814 1102 50-51 0.6563477622514213 1102 52-53 0.7762932323935701 1102 54-55 0.6010806537664948 1102 56-57 0.6067421993090605 1102 58-59 0.6509068303737706 1102 60-61 0.6793202244537255 1102 62-63 0.7495000530213289 1102 64-65 0.5238050290965717 1102 66-67 0.6244819318564581 1102 68-69 0.572003417348796 1102 70-71 0.6225075505714557 1102 72-73 0.6489763930652117 1102 74-75 0.6701272135852427 1102 76-77 0.5569224125766574 1102 78-79 0.7570978722160184 1102 80-81 0.7250518801107226 1102 82-83 0.7102652917503889 1102 84-85 0.7667212421155227 1102 86-87 0.5433612588471419 1102 88-89 0.6996233597562806 1102 90-91 0.8388486276975691 1102 92-93 0.7978066787805389 1102 94-95 0.7815513658016897 1102 96-97 0.7880473434341546 1102 98-99 0.8876739835879981 1102 100-101 0.8874447937753835 1103 1 0.06510707072876443 1103 2 -0.17837232017392424 1103 3 0.0038910885380687432 1103 4 0.08710117397566108 1103 5 0.09569644660930265 1103 6 0.18665173915723443 1103 7 0.18473213032977043 1103 8 0.2387195704352365 1103 9 0.1956195650763064 1103 10-11 0.2334331849401181 1103 12-13 0.31399512799384155 1103 14-15 0.30111185379423233 1103 16-17 0.41363682493115306 1103 18-19 0.3742376028512311 1103 20-21 0.28285597021532993 1103 22-23 0.378884083408046 1103 24-25 0.3705896346768398 1103 26-27 0.35499436234168513 1103 28-29 0.3462255132951242 1103 30-31 0.3430896962838048 1103 32-33 0.36609507864638857 1103 34-35 0.3880412399807298 1103 36-37 0.4662486090785123 1103 38-39 0.44294599309766625 1103 40-41 0.406836183152393 1103 42-43 0.3422888402537154 1103 44-45 0.44175127164406547 1103 46-47 0.4658738352326992 1103 48-49 0.3461776904807081 1103 50-51 0.20811961453588168 1103 52-53 0.3700256437560441 1103 54-55 0.3062409700234454 1103 56-57 0.4638700709491985 1103 58-59 0.42787622810197234 1103 60-61 0.38182733360253707 1103 62-63 0.19354803505478202 1103 64-65 -0.0018430618194713588 1103 66-67 0.20367340493104535 1103 68-69 0.19411795962277978 1103 70-71 0.18641147504267508 1103 72-73 0.30037535471957 1103 74-75 0.1945088074531789 1103 76-77 0.3099331497633777 1103 78-79 0.2512738741983789 1103 80-81 0.39073223969962 1103 82-83 0.4316475709706751 1103 84-85 0.4458769736969117 1103 86-87 0.22791759453345506 1103 88-89 0.4681814161928344 1103 90-91 0.833313877846571 1103 92-93 0.7277190384283969 1103 94-95 0.7676421215592448 1103 96-97 0.9586136019906952 1103 98-99 0.8957920465832991 1103 100-101 0.9838925913900987 1104 1 0.17997043154080927 1104 2 0.0732487166218263 1104 3 0.11740145983456074 1104 4 0.12555714744176072 1104 5 0.0632351326025784 1104 6 0.10078455519332863 1104 7 0.1618646365811145 1104 8 0.20236368712206598 1104 9 0.195882511110689 1104 10-11 0.28690084953412764 1104 12-13 0.25950988079674175 1104 14-15 0.2677676764489547 1104 16-17 0.2863782483456845 1104 18-19 0.3314425560681755 1104 20-21 0.25097167667294684 1104 22-23 0.3426826287182436 1104 24-25 0.2975926620343543 1104 26-27 0.24984203450408415 1104 28-29 0.37729059313425495 1104 30-31 0.34148883259651086 1104 32-33 0.37669685870292113 1104 34-35 0.4019052667372307 1104 36-37 0.42059732838756503 1104 38-39 0.3013156998595079 1104 40-41 0.4250060091906249 1104 42-43 0.4610594075493424 1104 44-45 0.5791956448771813 1104 46-47 0.605273029050359 1104 48-49 0.572166358294993 1104 50-51 0.4494228019797717 1104 52-53 0.458840072490716 1104 54-55 0.4979591206270584 1104 56-57 0.48367380283514194 1104 58-59 0.3966409521524028 1104 60-61 0.3318325739732373 1104 62-63 0.5693214534830773 1104 64-65 0.5349015596124431 1104 66-67 0.6065526408200697 1104 68-69 0.48690651391116546 1104 70-71 0.4878272794442182 1104 72-73 0.6143052107345781 1104 74-75 0.533286947780109 1104 76-77 0.3664650399697287 1104 78-79 0.6496689990768765 1104 80-81 0.7508657022026846 1104 82-83 0.7301292315282097 1104 84-85 0.9067374332913332 1104 86-87 0.8503883041074758 1104 88-89 0.9203836703869896 1104 90-91 1.078636615119425 1104 92-93 1.008452189477211 1104 94-95 0.8058643652170083 1104 96-97 0.8357883558323955 1104 98-99 0.8491447476465837 1104 100-101 0.9051466306492877 1105 1 0.23465250326632514 1105 2 0.08215314890801295 1105 3 0.1218213489352209 1105 4 0.14447932381713002 1105 5 0.11803426097332448 1105 6 0.17789916832657582 1105 7 0.2659521333302166 1105 8 0.292214791069334 1105 9 0.260023002374993 1105 10-11 0.25035594684762685 1105 12-13 0.2876256739580185 1105 14-15 0.45226077629330064 1105 16-17 0.4041272260105586 1105 18-19 0.3985992271081855 1105 20-21 0.3045340682287545 1105 22-23 0.3620360344964979 1105 24-25 0.3537454019119295 1105 26-27 0.4551344626252387 1105 28-29 0.3670800391615643 1105 30-31 0.4800082079579937 1105 32-33 0.48034645442687207 1105 34-35 0.4377426742078967 1105 36-37 0.2907050884304212 1105 38-39 0.4419887961909339 1105 40-41 0.3177035789733722 1105 42-43 0.45524642306827445 1105 44-45 0.44407327762063176 1105 46-47 0.4661838971472889 1105 48-49 0.564251509544313 1105 50-51 0.43472794742599064 1105 52-53 0.5767739147434838 1105 54-55 0.6204769738882447 1105 56-57 0.685241100338061 1105 58-59 0.6144153790236615 1105 60-61 0.6196542432082808 1105 62-63 0.5185889214884085 1105 64-65 0.4738690132555803 1105 66-67 0.5611523725965881 1105 68-69 0.49649970021973644 1105 70-71 0.3529546734648932 1105 72-73 0.5169345213303842 1105 74-75 0.5382592472044543 1105 76-77 0.5890700852688333 1105 78-79 0.4712780075622085 1105 80-81 0.7141574054217514 1105 82-83 0.8297146569205509 1105 84-85 0.8724249160664144 1105 86-87 0.7637222326303821 1105 88-89 0.9394989599014281 1105 90-91 0.9922665817176437 1105 92-93 1.0745529246657242 1105 94-95 1.065881017586186 1105 96-97 1.3637750834283615 1105 98-99 1.2684862294163146 1105 100-101 0.9754406017347215 1106 1 0.33765649547687104 1106 2 0.21994576956888068 1106 3 0.23356654474964955 1106 4 0.20060709749171224 1106 5 0.20556155617899918 1106 6 0.22531627347504468 1106 7 0.2666973039137801 1106 8 0.32277452628290604 1106 9 0.4375783153064958 1106 10-11 0.38992282755611285 1106 12-13 0.37043021046707025 1106 14-15 0.6171838852401592 1106 16-17 0.6047785979960381 1106 18-19 0.5684555430811642 1106 20-21 0.5307387845650524 1106 22-23 0.5414369993476171 1106 24-25 0.6419982564399405 1106 26-27 0.5472477537848022 1106 28-29 0.6634887699574321 1106 30-31 0.599465231197911 1106 32-33 0.5807740315300975 1106 34-35 0.5560792177861771 1106 36-37 0.5872275731428118 1106 38-39 0.7499764141452161 1106 40-41 0.7097212939059148 1106 42-43 0.7230942477091844 1106 44-45 0.7909682472747761 1106 46-47 0.5885553717077059 1106 48-49 0.6130473523009883 1106 50-51 0.5446869128688832 1106 52-53 0.6066453921710391 1106 54-55 0.7172398399077764 1106 56-57 0.7220916350029682 1106 58-59 0.7376436994051474 1106 60-61 0.691785121425788 1106 62-63 0.7727898600764789 1106 64-65 0.7864781080639816 1106 66-67 0.7672513171437458 1106 68-69 0.8033869085165577 1106 70-71 0.8308592474761838 1106 72-73 0.9192190468984194 1106 74-75 0.9317322524754097 1106 76-77 0.8412090459637334 1106 78-79 0.9415177753006567 1106 80-81 0.8836860068979888 1106 82-83 1.0665740367230185 1106 84-85 0.9498037419826417 1106 86-87 0.8039815858257562 1106 88-89 1.0282289501072697 1106 90-91 0.9856202564780681 1106 92-93 1.1358560356310576 1106 94-95 1.210693910671555 1106 96-97 1.4374835356525786 1106 98-99 1.5729365808134155 1106 100-101 1.6356661111687707 1107 1 0.2063519447644282 1107 2 0.09223137869659581 1107 3 0.1181556398650585 1107 4 0.07845950823885772 1107 5 0.13445831731260682 1107 6 0.07479245518807431 1107 7 0.1025489522654297 1107 8 0.10995445162072315 1107 9 0.16328681733078554 1107 10-11 0.16755884285660017 1107 12-13 0.18659759571340828 1107 14-15 0.2047624251213378 1107 16-17 0.19932796310461498 1107 18-19 0.2811260962122404 1107 20-21 0.19319803584009065 1107 22-23 0.1749638343547133 1107 24-25 0.1765229422804211 1107 26-27 0.24305194958900245 1107 28-29 0.16592054870500306 1107 30-31 0.2070969087309038 1107 32-33 0.20782296943429657 1107 34-35 0.2703279624625594 1107 36-37 0.16424677105016627 1107 38-39 0.25316155137641516 1107 40-41 0.25356478594019904 1107 42-43 0.36181900978921533 1107 44-45 0.343266278191237 1107 46-47 0.3393958141599853 1107 48-49 0.33811258312280046 1107 50-51 0.35552738818304164 1107 52-53 0.4671089417793226 1107 54-55 0.21959685788979044 1107 56-57 0.30984927210270996 1107 58-59 0.3952336423569349 1107 60-61 0.43152728715479327 1107 62-63 0.3658284004834442 1107 64-65 0.41637617052783327 1107 66-67 0.28361570933050473 1107 68-69 0.43191380924740486 1107 70-71 0.3844577870944619 1107 72-73 0.2928495282591612 1107 74-75 0.2952108791119308 1107 76-77 0.3016490993314207 1107 78-79 0.5159482790074712 1107 80-81 0.5757423058621818 1107 82-83 0.4947963078729174 1107 84-85 0.5263612305467085 1107 86-87 0.5768723463809913 1107 88-89 0.6609063056464635 1107 90-91 0.8706201250305661 1107 92-93 0.8708825223022814 1107 94-95 0.8505959494222743 1107 96-97 1.044896681717038 1107 98-99 1.036185502943919 1107 100-101 0.972331912472729 1108 1 0.08786280559659332 1108 2 -0.08688452269658242 1108 3 0.057842801352727236 1108 4 0.09445902708964127 1108 5 0.0784280990499866 1108 6 0.11824925079967841 1108 7 0.12399500177541967 1108 8 0.11335086823579843 1108 9 0.15906224877977593 1108 10-11 0.0998671627502219 1108 12-13 0.17137223025900283 1108 14-15 0.13508384717875543 1108 16-17 0.22576247091707558 1108 18-19 0.2570118912622874 1108 20-21 0.1611293753242009 1108 22-23 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-1.278227382484225 2316 76-77 -1.5368084601787722 2316 78-79 -1.3456838744009971 2316 80-81 -1.525839265127285 2316 82-83 -1.3452689344949533 2316 84-85 -1.340751057947159 2316 86-87 -1.2518373418681712 2316 88-89 -1.4091251653718473 2316 90-91 -1.5296877463299374 2316 92-93 -1.7970107597357412 2316 94-95 -1.589502430149789 2316 96-97 -1.490915683371643 2316 98-99 -1.447232776855941 2316 100-101 -1.2232196820920258 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 7.0 4 25.0 5 62.0 6 131.0 7 288.0 8 692.0 9 1325.0 10 2209.0 11 3386.0 12 5069.0 13 7053.0 14 9121.0 15 12239.0 16 15932.0 17 21047.0 18 19418.0 19 13478.0 20 12721.0 21 13609.0 22 14233.0 23 16347.0 24 19084.0 25 23371.0 26 28826.0 27 36064.0 28 45441.0 29 57005.0 30 70976.0 31 89269.0 32 113397.0 33 147293.0 34 200045.0 35 279462.0 36 420669.0 37 613288.0 38 423123.0 39 27593.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.28328883818437 19.5656076628175 15.144408957713202 23.006694541284936 2 15.635541404547102 20.75085833112274 39.658028286656354 23.955571977673802 3 19.499384055505935 24.480645156620714 29.65544745363769 26.364523334235656 4 13.613216082220534 17.275105851699056 36.77248217710708 32.33919588897333 5 14.342210496983645 37.04571033594256 33.8483391054801 14.763740061593692 6 32.14563075174882 36.15773226841917 17.42897053874186 14.26766644109015 7 28.842372830446507 32.220183194073456 20.719618108644774 18.21782586683526 8 26.38825810145185 35.10138779669678 19.70020638359701 18.810147718254363 9 26.145070696243373 17.030717971006414 20.156291334168806 36.667919998581404 10-11 24.807625811100102 26.78444736084525 27.477911822442703 20.930015005611953 12-13 25.82939945090607 23.90103860286301 28.03820605190318 22.231355894327738 14-15 22.78324416761767 25.310367077341027 26.04846993658489 25.85791881845641 16-17 22.45537131134104 28.685673402933666 26.493892630589283 22.365062655136015 18-19 22.335930830553746 27.238997850758928 28.75394320054167 21.671128118145653 20-21 22.905370316421454 26.428329735510243 28.95325966390964 21.713040284158662 22-23 22.764150557612073 26.386340105547678 28.77885905299495 22.0706502838453 24-25 22.652582520673935 26.54167638516409 28.78583049408449 22.019910600077484 26-27 22.567336861180802 26.776778452204418 28.588447852666377 22.0674368339484 28-29 22.34929390502261 26.664938010861217 28.83703685474876 22.148731229367414 30-31 22.54462148806037 26.450204017295732 28.8283759130197 22.1767985816242 32-33 22.35627970197779 26.63146459068268 28.734849548580605 22.277406158758925 34-35 22.72965218167017 26.610279930595933 28.584810902135306 22.0752569855986 36-37 22.76852350636409 26.4105528430187 28.49617933709793 22.32474431351928 38-39 22.556053844116104 26.578149676683427 28.728146998663885 22.137649480536588 40-41 22.749391463332543 26.532632982361665 28.568127516569643 22.14984803773615 42-43 22.65297769362554 26.531912106269225 28.40812942459701 22.406980775508227 44-45 22.748663635728686 26.721459019187698 28.18841080585 22.341466539233615 46-47 22.77516425562991 26.795466796125428 27.933526568509855 22.495842379734807 48-49 22.915282071918888 26.917716123893463 27.698523519126724 22.468478285060925 50-51 22.776773040181162 26.982092952351312 27.685672642920277 22.55546136454725 52-53 23.04603021345709 27.059424479385306 27.455929058015283 22.43861624914232 54-55 22.862439472788992 26.882930059857703 27.570406988947678 22.68422347840563 56-57 22.81265346284557 27.21770888408061 27.495982145926725 22.473655507147093 58-59 22.99375938991156 26.924697772187784 27.498944638628526 22.58259819927213 60-61 22.90970631917524 26.847266573829863 27.86348937057228 22.379537736422616 62-63 22.884260527535726 26.895079471979344 27.85438502718307 22.36627497330186 64-65 22.791103744710803 26.917333367109403 27.655762725511824 22.63580016266797 66-67 22.750796963486785 27.15243471485734 27.511244703345746 22.58552361831013 68-69 22.63236976355453 26.99329533771384 27.710137259748397 22.66419763898323 70-71 22.687382974981347 26.98634023547225 27.616167347857523 22.710109441688882 72-73 22.775920095537945 26.98409510368038 27.496580175876666 22.743404624905004 74-75 22.671739371168965 27.13671331885299 27.397562177783986 22.79398513219406 76-77 22.740374750432366 27.091817366252197 27.458483200574392 22.709324682741048 78-79 22.7618740532133 27.052550325806806 27.346293952073143 22.839281668906747 80-81 22.651220788132107 27.246604287456343 27.3680959092573 22.734079015154244 82-83 22.739927397428332 27.201790057623903 27.39203615435809 22.666246390589677 84-85 22.753649229223655 27.145463056603695 27.30487849232445 22.7960092218482 86-87 22.751914321210776 26.928498352122194 27.48568624421017 22.83390108245686 88-89 22.763722467171586 26.950719066670842 27.290769141410205 22.99478932474737 90-91 22.670975528331642 26.99702076956969 27.525827385578282 22.80617631652039 92-93 22.73149445744319 26.971008039118438 27.416471715616886 22.881025787821486 94-95 22.63919438177769 26.88702324110283 27.340214251541816 23.13356812557766 96-97 22.67556918678555 26.881991848403835 27.30138739985796 23.14105156495266 98-99 22.508167517572264 26.90222411613934 27.18817544307256 23.401432923215832 100-101 22.242588484826662 27.124731456352038 27.30504224230259 23.327637816518713 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 474.0 1 354.5 2 250.5 3 293.0 4 264.0 5 233.5 6 430.0 7 999.0 8 1746.0 9 2080.5 10 1885.0 11 1695.0 12 1760.5 13 1965.5 14 2224.5 15 2464.0 16 2671.0 17 2891.0 18 3129.0 19 3352.0 20 3691.5 21 4299.5 22 5101.5 23 6105.0 24 7528.0 25 9462.5 26 12303.5 27 15950.0 28 19283.0 29 22944.0 30 28297.0 31 33890.5 32 38861.5 33 44467.5 34 51812.0 35 58094.5 36 64328.0 37 71067.5 38 75200.0 39 77746.0 40 80728.0 41 86161.0 42 91724.0 43 96915.5 44 102050.5 45 106095.5 46 107896.0 47 109725.0 48 113562.0 49 113507.5 50 110327.0 51 106546.5 52 101500.0 53 98357.0 54 94736.5 55 87948.0 56 80474.5 57 72182.5 58 64483.0 59 56881.5 60 47766.0 61 38427.0 62 30001.5 63 22828.5 64 16943.5 65 12669.0 66 9435.0 67 7338.5 68 5317.5 69 3346.5 70 2182.0 71 1382.5 72 881.5 73 574.0 74 388.5 75 271.0 76 164.0 77 85.5 78 49.0 79 29.0 80 20.0 81 11.5 82 7.5 83 6.0 84 5.0 85 3.5 86 3.0 87 2.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01639343669039312 2 0.0032569741768992957 3 0.0023522591277606022 4 3.618860196554773E-5 5 0.0011218466609319796 6 3.9807462162102505E-4 7 7.237720393109546E-5 8 0.0 9 0.0 10-11 5.428290294832159E-5 12-13 7.780549422592761E-4 14-15 1.8094300982773864E-5 16-17 0.0 18-19 0.0 20-21 0.0020265617100706725 22-23 0.0 24-25 0.003600765895571999 26-27 0.0017551471953290649 28-29 0.0027141451474160796 30-31 0.015289684330443917 32-33 6.152062334143114E-4 34-35 0.002261787622846733 36-37 0.003709331701468642 38-39 0.0023522591277606022 40-41 0.004161689226037989 42-43 0.001990373108105125 44-45 7.237720393109545E-4 46-47 5.428290294832159E-5 48-49 2.7141451474160794E-4 50-51 1.2666010687941703E-4 52-53 0.001049469457000884 54-55 0.002931276759209366 56-57 3.6188601965547724E-4 58-59 0.001067563757983658 60-61 0.001049469457000884 62-63 0.0 64-65 1.2666010687941703E-4 66-67 0.0 68-69 0.0 70-71 1.0856580589664318E-4 72-73 3.618860196554773E-5 74-75 5.428290294832159E-4 76-77 7.961492432420501E-4 78-79 2.5332021375883406E-4 80-81 0.00533781878991829 82-83 0.0013751668746908136 84-85 0.0029131824582265924 86-87 0.0018094300982773863 88-89 0.0011399409619147535 90-91 1.2666010687941703E-4 92-93 1.8094300982773864E-5 94-95 2.5332021375883406E-4 96-97 0.0013932611756735875 98-99 0.0024789192346400192 100-101 5.971119324315375E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2763301.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.35200953996993 #Duplication Level Percentage of deduplicated Percentage of total 1 76.31255157612325 42.24053082861022 2 13.386455221539872 14.819343942581106 3 4.35984583022095 7.239786839615644 4 1.9244356524022017 4.260855223632998 5 1.0372689799103811 2.870746123575715 6 0.6351428372826732 2.109385943310846 7 0.410858569952243 1.5919293238502472 8 0.29649287809714664 1.312918129357312 9 0.232002377383471 1.1557618025603031 >10 1.2607763331128807 12.794223360951612 >50 0.09554150875820856 3.6240914340182027 >100 0.0465951096366321 4.540889804423943 >500 0.0015740327071041378 0.5531766913143701 >1k 3.2792348062423717E-4 0.3448006440354539 >5k 1.3116939224969487E-4 0.5415599081618132 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8435 0.3052508575793951 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6483 0.2346107065426459 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4236 0.15329491792606015 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.237720393109546E-5 7.237720393109546E-5 10-11 0.0 0.0 0.0 9.047150491386932E-5 7.237720393109546E-5 12-13 0.0 0.0 0.0 1.0856580589664318E-4 7.237720393109546E-5 14-15 0.0 0.0 0.0 1.0856580589664318E-4 9.047150491386932E-5 16-17 0.0 0.0 0.0 1.0856580589664318E-4 1.0856580589664318E-4 18-19 0.0 0.0 0.0 1.447544078621909E-4 1.447544078621909E-4 20-21 0.0 0.0 0.0 2.1713161179328635E-4 1.447544078621909E-4 22-23 0.0 0.0 0.0 5.066404275176682E-4 1.6284870884496476E-4 24-25 0.0 0.0 0.0 7.599606412765022E-4 1.8094300982773864E-4 26-27 0.0 0.0 0.0 9.409036511042409E-4 1.8094300982773864E-4 28-29 0.0 0.0 0.0 0.002225599020881185 1.8094300982773864E-4 30-31 0.0 0.0 0.0 0.004035029119158571 1.8094300982773864E-4 32-33 0.0 0.0 0.0 0.0070205887813162594 1.8094300982773864E-4 34-35 0.0 0.0 0.0 0.009752828229715113 1.8094300982773864E-4 36-37 0.0 0.0 0.0 0.012105087357475714 2.1713161179328635E-4 38-39 0.0 0.0 0.0 0.014077366164598065 2.1713161179328635E-4 40-41 0.0 0.0 0.0 0.016701039807100276 2.3522591277606023E-4 42-43 0.0 1.8094300982773864E-5 0.0 0.019505656459430226 2.533202137588341E-4 44-45 0.0 3.618860196554773E-5 0.0 0.02321498816089887 2.7141451474160794E-4 46-47 0.0 3.618860196554773E-5 0.0 0.027358583085954083 2.895088157243818E-4 48-49 0.0 3.618860196554773E-5 0.0 0.03247927026407908 2.895088157243818E-4 50-51 0.0 3.618860196554773E-5 0.0 0.03975317925915418 2.895088157243818E-4 52-53 0.0 3.618860196554773E-5 0.0 0.057196085406548185 3.9807462162102505E-4 54-55 0.0 3.618860196554773E-5 0.0 0.0796873015281361 5.066404275176682E-4 56-57 0.0 3.618860196554773E-5 0.0 0.106340206875762 5.066404275176682E-4 58-59 0.0 3.618860196554773E-5 0.0 0.13702814134254648 5.066404275176682E-4 60-61 0.0 3.618860196554773E-5 0.0 0.17954974865206505 5.066404275176682E-4 62-63 0.0 3.618860196554773E-5 0.0 0.21154047278960925 5.428290294832159E-4 64-65 0.0 7.237720393109546E-5 0.0 0.24320549950946352 5.428290294832159E-4 66-67 0.0 7.237720393109546E-5 0.0 0.3013967714700643 5.609233304659897E-4 68-69 0.0 7.237720393109546E-5 0.0 0.37936511440483683 5.790176314487636E-4 70-71 0.0 7.237720393109546E-5 0.0 0.4418990186013033 6.333005343970852E-4 72-73 0.0 7.237720393109546E-5 0.0 0.5035463020496138 8.685264471731454E-4 74-75 0.0 7.237720393109546E-5 0.0 0.6139215380445344 8.685264471731454E-4 76-77 0.0 7.237720393109546E-5 0.0 0.6976076800898636 8.685264471731454E-4 78-79 0.0 7.237720393109546E-5 0.0 0.767017418659784 8.685264471731454E-4 80-81 0.0 7.237720393109546E-5 0.0 0.8553899846596515 8.866207481559192E-4 82-83 0.0 7.237720393109546E-5 0.0 0.9995291862884282 9.047150491386932E-4 84-85 0.0 7.237720393109546E-5 0.0 1.1545611571088346 9.228093501214671E-4 86-87 0.0 7.237720393109546E-5 0.0 1.351951886529915 9.409036511042409E-4 88-89 0.0 7.237720393109546E-5 1.8094300982773864E-5 1.59510310313643 9.409036511042409E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4995 0.0 45.464428 1 GTATCAA 9030 0.0 40.774933 1 ATCAACG 11465 0.0 31.859379 3 TCAACGC 11495 0.0 31.651121 4 CAACGCA 11730 0.0 31.057508 5 TATCAAC 11765 0.0 30.967356 2 AACGCAG 12185 0.0 30.053167 6 ACGCAGA 14020 0.0 25.81478 7 CGCAGAG 14210 0.0 25.269062 8 GTACATG 9955 0.0 23.19393 1 GCAGAGT 15365 0.0 22.874973 9 TACATGG 10375 0.0 22.159399 2 GAGTACT 8280 0.0 22.084717 12-13 ACATGGG 10290 0.0 21.279947 3 GTACTTT 8695 0.0 20.840023 14-15 AGAGTAC 13720 0.0 20.632584 10-11 GTGGTAT 3090 0.0 20.44902 1 AGTACTT 8850 0.0 19.937788 12-13 TGGTATC 3005 0.0 19.917131 2 CAGAGTA 14915 0.0 18.613272 10-11 >>END_MODULE