FastQCFastQC Report
Thu 26 May 2016
SRR936850_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936850_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550142
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76160.2986500359587819No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51520.20202796550152893No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34390.13485523551237538No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42550.044.1213951
GTATCAA78700.043.4821
ATCAACG98850.034.233123
TCAACGC99350.034.1086774
TATCAAC100600.033.5437852
CAACGCA101900.033.1637955
AACGCAG104050.032.5673266
ACGCAGA122150.027.4329767
CGCAGAG122000.027.038688
GTACATG91700.025.500431
GCAGAGT130400.024.7137099
TACATGG94850.024.2025722
ACATGGG94150.023.2714673
CATGGGG53400.021.6275944
AGAGTAC115500.021.02743710-11
GAGTACT67650.020.54849212-13
AGTACTT72800.019.58446712-13
GTACTTT71200.019.4566314-15
CAGAGTA125500.019.16259210-11
GTACGGG2752.8232535E-819.010556
Produced by FastQC (version 0.11.5)