##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936850_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2550142 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78791965310167 33.0 31.0 34.0 30.0 34.0 2 31.91272407575735 33.0 31.0 34.0 30.0 34.0 3 31.93364016592017 33.0 31.0 34.0 30.0 34.0 4 35.51815663598341 37.0 35.0 37.0 33.0 37.0 5 35.51576500445857 37.0 35.0 37.0 33.0 37.0 6 35.58190681146383 37.0 35.0 37.0 33.0 37.0 7 35.51852485077301 37.0 35.0 37.0 33.0 37.0 8 35.46734495569266 37.0 35.0 37.0 33.0 37.0 9 37.1150022234056 39.0 37.0 39.0 33.0 39.0 10-11 36.92667898493495 39.0 37.0 39.0 33.0 39.0 12-13 36.855003368439874 39.0 37.0 39.0 33.0 39.0 14-15 38.033777138684826 40.0 38.0 41.0 33.0 41.0 16-17 37.96443452952816 40.0 38.0 41.0 33.0 41.0 18-19 37.903597917292444 40.0 38.0 41.0 33.0 41.0 20-21 37.82082311494811 40.0 38.0 41.0 32.5 41.0 22-23 37.71125274592552 40.0 37.0 41.0 32.0 41.0 24-25 37.5491470671045 40.0 37.0 41.0 32.0 41.0 26-27 37.66283995165759 40.0 37.0 41.0 32.0 41.0 28-29 37.61881926575069 40.0 37.0 41.0 32.0 41.0 30-31 37.50353490119374 40.0 37.0 41.0 32.0 41.0 32-33 37.32703923938353 40.0 37.0 41.0 31.5 41.0 34-35 37.176910932802954 40.0 36.5 41.0 31.0 41.0 36-37 37.01285124514635 39.5 36.0 41.0 31.0 41.0 38-39 36.86535043930887 39.0 36.0 41.0 30.0 41.0 40-41 36.70332377569562 39.0 36.0 41.0 30.0 41.0 42-43 36.50641728970386 39.0 35.5 41.0 30.0 41.0 44-45 36.28566919018627 39.0 35.0 40.5 30.0 41.0 46-47 35.98635056400781 39.0 35.0 40.0 29.0 41.0 48-49 35.703086730072286 39.0 35.0 40.0 28.0 41.0 50-51 35.076334572741445 38.0 34.0 39.5 27.0 40.5 52-53 35.328326618674566 38.5 35.0 40.0 28.0 40.5 54-55 35.706978866274895 39.0 35.0 40.5 28.0 41.0 56-57 35.599138989122956 39.0 35.0 41.0 28.0 41.0 58-59 35.35141219586988 38.5 35.0 40.0 27.5 41.0 60-61 35.06282042333329 38.0 34.0 40.0 26.5 41.0 62-63 34.730121303049 38.0 34.0 40.0 26.0 41.0 64-65 34.415403926526444 37.0 34.0 40.0 26.0 41.0 66-67 34.03993405073129 37.0 34.0 39.5 26.0 41.0 68-69 33.65082709119727 36.0 33.0 39.0 25.0 41.0 70-71 33.241749675116125 36.0 33.0 39.0 24.5 40.5 72-73 32.847325952829294 35.0 32.5 38.5 23.5 40.0 74-75 32.40815452629697 35.0 32.0 37.0 22.0 39.5 76-77 31.972446436316094 35.0 32.0 37.0 21.5 39.0 78-79 31.532500347039495 35.0 31.0 36.0 20.0 39.0 80-81 31.092554649897927 35.0 31.0 36.0 18.5 37.5 82-83 30.683677418747664 34.5 31.0 35.5 17.5 37.0 84-85 30.331485266310658 34.0 30.5 35.0 14.5 37.0 86-87 29.996074336252647 34.0 30.0 35.0 9.5 36.0 88-89 29.659980306978984 34.0 30.0 35.0 7.0 36.0 90-91 29.315962797365792 34.0 29.5 35.0 2.0 35.5 92-93 28.942283606167813 34.0 29.0 35.0 2.0 35.0 94-95 28.511938746940366 34.0 29.0 35.0 2.0 35.0 96-97 28.027827469999707 34.0 29.0 35.0 2.0 35.0 98-99 27.40269267358445 34.0 27.5 35.0 2.0 35.0 100-101 25.078227800647966 31.5 22.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3318167017303608 1101 2 0.41884422303639823 1101 3 0.3440872969468174 1101 4 0.23850131876033487 1101 5 0.18656431580959065 1101 6 0.18206972942908095 1101 7 0.16858015422594264 1101 8 0.21066448091400503 1101 9 0.23803434650152866 1101 10-11 0.24180757513025952 1101 12-13 0.22875700133155874 1101 14-15 0.5674848936664318 1101 16-17 0.613076607179714 1101 18-19 0.4989125399428076 1101 20-21 0.636758967814508 1101 22-23 0.6027077728526891 1101 24-25 0.5769700380924405 1101 26-27 0.5635985707432454 1101 28-29 0.6542712635465904 1101 30-31 0.4970995587375313 1101 32-33 0.7787301173364938 1101 34-35 0.6491956993968984 1101 36-37 0.6050709758323265 1101 38-39 0.6734824046363386 1101 40-41 0.6781616537186395 1101 42-43 0.6585874689819136 1101 44-45 0.6793444268176501 1101 46-47 0.5850991986943441 1101 48-49 0.6015786744201463 1101 50-51 0.5220853122311269 1101 52-53 0.5929481082137968 1101 54-55 0.6889802931142768 1101 56-57 0.6939266257955978 1101 58-59 0.6431147177089045 1101 60-61 0.7656677661842508 1101 62-63 0.5968259667800027 1101 64-65 0.8160035897392817 1101 66-67 0.812468949358383 1101 68-69 0.6578967168170422 1101 70-71 0.7580503158837573 1101 72-73 0.7878452172705508 1101 74-75 0.7064946034136526 1101 76-77 0.7515159255877393 1101 78-79 0.8035747822230235 1101 80-81 0.7412508631064476 1101 82-83 0.7413959542506632 1101 84-85 0.7523509468503455 1101 86-87 0.7568144473962448 1101 88-89 0.6842390382041081 1101 90-91 0.6942658601836165 1101 92-93 0.7111184537574609 1101 94-95 0.833055424607597 1101 96-97 0.8721786745548563 1101 98-99 0.8119519784734379 1101 100-101 1.0344420112315156 1102 1 0.3234550875073907 1102 2 0.2622306465486979 1102 3 0.13933931021797719 1102 4 0.21915576681740134 1102 5 0.10013119531935644 1102 6 0.07939711122168092 1102 7 0.11452460941205089 1102 8 0.19103946119349047 1102 9 0.25385001638792914 1102 10-11 0.4072345285874803 1102 12-13 0.2867065310641479 1102 14-15 0.3195534415608705 1102 16-17 0.3987236355808008 1102 18-19 0.3103932565475347 1102 20-21 0.37176918689304017 1102 22-23 0.2612721672818381 1102 24-25 0.38409219611509116 1102 26-27 0.3392934740148874 1102 28-29 0.23507772262104965 1102 30-31 0.2962654850201929 1102 32-33 0.36006728050131187 1102 34-35 0.24216748809742938 1102 36-37 0.2123782397407723 1102 38-39 0.3750243408166227 1102 40-41 0.30910333913708143 1102 42-43 0.18891624692721365 1102 44-45 0.34963312320011397 1102 46-47 0.2256793197100606 1102 48-49 0.4006033840690222 1102 50-51 0.4423196808367891 1102 52-53 0.46071020711644195 1102 54-55 0.5361159263234967 1102 56-57 0.6298121389024658 1102 58-59 0.5646672466194573 1102 60-61 0.464318883402953 1102 62-63 0.505969284166909 1102 64-65 0.5016707834723704 1102 66-67 0.33708358393291604 1102 68-69 0.24193639032845482 1102 70-71 0.44411844386481647 1102 72-73 0.5063142889891452 1102 74-75 0.5912399449254124 1102 76-77 0.4800361698017781 1102 78-79 0.47535873797316697 1102 80-81 0.4230088886120349 1102 82-83 0.3249577687035128 1102 84-85 0.3741258282010911 1102 86-87 0.3104239232043682 1102 88-89 0.19765681287668713 1102 90-91 0.43693238134357415 1102 92-93 0.505444700360492 1102 94-95 0.4891356280639343 1102 96-97 0.49483400988653514 1102 98-99 0.5024434313381612 1102 100-101 0.5501547193051195 1103 1 0.24094139500910217 1103 2 0.2917262622363701 1103 3 0.2622736796697609 1103 4 0.22955151543609986 1103 5 0.21973957630389407 1103 6 0.07120060239427772 1103 7 0.08480593486396515 1103 8 0.20348433701903446 1103 9 0.19093656624007593 1103 10-11 0.35837860953976275 1103 12-13 0.3308423437670456 1103 14-15 0.3211709197297381 1103 16-17 0.39033089524242115 1103 18-19 0.31729903829348416 1103 20-21 0.3773170224685245 1103 22-23 0.4479395774780386 1103 24-25 0.27551950153392113 1103 26-27 0.3921445581110916 1103 28-29 0.3866227066602832 1103 30-31 0.3353809662093994 1103 32-33 0.4105608735876842 1103 34-35 0.41657419674337604 1103 36-37 0.3109440315545058 1103 38-39 0.30634542198894366 1103 40-41 0.3588828424392574 1103 42-43 0.4731887886829256 1103 44-45 0.40650529540155134 1103 46-47 0.450352342549472 1103 48-49 0.4735764873904813 1103 50-51 0.38160667620379485 1103 52-53 0.5812261965181378 1103 54-55 0.5741917723646566 1103 56-57 0.5815940476231631 1103 58-59 0.5489904287651086 1103 60-61 0.5586239495519649 1103 62-63 0.5867404046759646 1103 64-65 0.5454174488931258 1103 66-67 0.5405180875214484 1103 68-69 0.47577683089984646 1103 70-71 0.3053577468360231 1103 72-73 0.3564763080938391 1103 74-75 0.3299382457594717 1103 76-77 0.4324836379329966 1103 78-79 0.46947993127778176 1103 80-81 0.5041426304847043 1103 82-83 0.6156969073948808 1103 84-85 0.41340950782828045 1103 86-87 0.39095691330839344 1103 88-89 0.3986002915700446 1103 90-91 0.493334924214583 1103 92-93 0.45719737028461793 1103 94-95 0.3740466004498515 1103 96-97 0.5983028765968079 1103 98-99 0.7018818655855306 1103 100-101 0.6996101843979154 1104 1 0.3634504052302461 1104 2 0.239808608630927 1104 3 0.32807756658998954 1104 4 0.3378723026259891 1104 5 0.22960743059511657 1104 6 0.17768764651868452 1104 7 0.3011046828544295 1104 8 0.28494946609254157 1104 9 0.23057649770000666 1104 10-11 0.4819380927541914 1104 12-13 0.4536297113708372 1104 14-15 0.5816538764056816 1104 16-17 0.6028249635266292 1104 18-19 0.5451843099298372 1104 20-21 0.4903422195779399 1104 22-23 0.48276815483044544 1104 24-25 0.6581918332386181 1104 26-27 0.4278873601748572 1104 28-29 0.5233056974928232 1104 30-31 0.6623900769467355 1104 32-33 0.702744936067802 1104 34-35 0.5538718803335243 1104 36-37 0.5049156437649245 1104 38-39 0.6172922971327068 1104 40-41 0.6285720740985212 1104 42-43 0.476396358087932 1104 44-45 0.5488171930527486 1104 46-47 0.607303116083024 1104 48-49 0.6471569246075646 1104 50-51 0.6089818675469303 1104 52-53 0.5286066941079497 1104 54-55 0.7128775247141803 1104 56-57 0.5630723477840505 1104 58-59 0.6510262125298851 1104 60-61 0.6397802827968562 1104 62-63 0.6831826355934822 1104 64-65 0.6850604052917859 1104 66-67 0.5176772793334479 1104 68-69 0.45564939275196537 1104 70-71 0.46166874582834083 1104 72-73 0.5104940884316065 1104 74-75 0.48012040378857535 1104 76-77 0.4298521302972347 1104 78-79 0.589890705353298 1104 80-81 0.6941873050485619 1104 82-83 0.5930944391709154 1104 84-85 0.6596927088940667 1104 86-87 0.46852491298573185 1104 88-89 0.5512329020480564 1104 90-91 0.7285242255104549 1104 92-93 0.6959232030723115 1104 94-95 0.863486287267861 1104 96-97 0.8211301985996435 1104 98-99 1.0541310020600534 1104 100-101 0.9387377668605801 1105 1 0.17918335773114435 1105 2 0.2531649193542087 1105 3 0.23157252880661616 1105 4 0.22207637783289158 1105 5 0.3085729982453458 1105 6 0.309858241456908 1105 7 0.30730914039311585 1105 8 0.380466813459698 1105 9 0.26009194840170125 1105 10-11 0.3031947085932174 1105 12-13 0.31643257810947034 1105 14-15 0.335409723832818 1105 16-17 0.2890205374740944 1105 18-19 0.2682308569491454 1105 20-21 0.322547299342844 1105 22-23 0.3430689974596888 1105 24-25 0.4114650805098208 1105 26-27 0.13753823878827376 1105 28-29 0.19426984826648663 1105 30-31 -0.08437134802628066 1105 32-33 -0.0771570633024794 1105 34-35 -0.026247242252544822 1105 36-37 -0.07464216329996276 1105 38-39 -0.1360507631707577 1105 40-41 -0.15201165655999205 1105 42-43 0.0018425055330553164 1105 44-45 0.05212792383120046 1105 46-47 0.02378423938877461 1105 48-49 0.04463531536563181 1105 50-51 -0.07719543319075228 1105 52-53 -0.1317126091486145 1105 54-55 0.03436606975436263 1105 56-57 0.2364059247601773 1105 58-59 0.06857842705549189 1105 60-61 -0.0029259889435806485 1105 62-63 0.036728481671211455 1105 64-65 0.1772108903283609 1105 66-67 0.12252848855138865 1105 68-69 0.07686449560670638 1105 70-71 0.001171208494767484 1105 72-73 -0.0691944423648394 1105 74-75 0.0481815506339629 1105 76-77 0.04886460855679786 1105 78-79 0.0455847312319122 1105 80-81 0.13051032292069564 1105 82-83 0.09756247323592859 1105 84-85 0.01105748339328727 1105 86-87 0.12122381921698988 1105 88-89 0.04542108424788793 1105 90-91 0.05785760007564278 1105 92-93 0.16960087864505624 1105 94-95 0.062422973875754906 1105 96-97 0.04930636358704987 1105 98-99 0.1229240143846333 1105 100-101 0.29868813869697064 1106 1 0.20216335586616907 1106 2 0.22932528395696394 1106 3 0.2767282542821832 1106 4 0.09265288846325603 1106 5 0.13652024274367136 1106 6 0.14133048440108809 1106 7 0.20500065009098734 1106 8 0.27449150884699236 1106 9 0.24801331636141555 1106 10-11 0.35875010225851867 1106 12-13 0.3755445388206482 1106 14-15 0.45026891816328884 1106 16-17 0.6227346366908009 1106 18-19 0.5618023243742272 1106 20-21 0.5986841574803563 1106 22-23 0.4024548764849314 1106 24-25 0.5425971596469594 1106 26-27 0.3626155575747916 1106 28-29 0.3239031474085934 1106 30-31 0.5485081745399327 1106 32-33 0.4698446967683907 1106 34-35 0.4778226787968194 1106 36-37 0.40684892367007564 1106 38-39 0.5377207811845892 1106 40-41 0.5386966845440782 1106 42-43 0.36824467589859466 1106 44-45 0.34890870370190186 1106 46-47 0.3914972351614949 1106 48-49 0.4661368345437964 1106 50-51 0.5879729391516548 1106 52-53 0.569374030631316 1106 54-55 0.6288892576195906 1106 56-57 0.5215806302494315 1106 58-59 0.6990227963828559 1106 60-61 0.7056764059961225 1106 62-63 0.5297421076503213 1106 64-65 0.5722180976825868 1106 66-67 0.6316634700570418 1106 68-69 0.6410012061003059 1106 70-71 0.6608641285805561 1106 72-73 0.6843718380964319 1106 74-75 0.6431559266441553 1106 76-77 0.780903046484184 1106 78-79 0.6354585928196315 1106 80-81 0.6169115413715254 1106 82-83 0.7051404913883594 1106 84-85 0.6999348456959105 1106 86-87 0.7658842576417086 1106 88-89 0.7641497147340246 1106 90-91 0.7365859003056592 1106 92-93 0.6473459869690643 1106 94-95 0.6173061991959088 1106 96-97 0.7445722391931682 1106 98-99 0.5455756019452416 1106 100-101 0.41736664795968537 1107 1 0.13748041632011976 1107 2 0.14455127362609588 1107 3 0.20260036122726177 1107 4 0.24541709903019182 1107 5 0.23816662696032864 1107 6 0.09582118915925264 1107 7 0.14386137270211918 1107 8 0.23898561873022572 1107 9 0.2989359692162026 1107 10-11 0.4015223182703309 1107 12-13 0.4113860135216001 1107 14-15 0.2821340313451799 1107 16-17 0.36019324734702707 1107 18-19 0.32913601427630823 1107 20-21 0.42363310489692196 1107 22-23 0.3149820997865973 1107 24-25 0.510623201843579 1107 26-27 0.2924604723821105 1107 28-29 0.32463546446086156 1107 30-31 0.33092186267073487 1107 32-33 0.41417749561878736 1107 34-35 0.4834774352068294 1107 36-37 0.38810852010514196 1107 38-39 0.06499030814705264 1107 40-41 0.3582381739732625 1107 42-43 0.3088670267064302 1107 44-45 0.41900916530248367 1107 46-47 0.41342954438460566 1107 48-49 0.36086932955653594 1107 50-51 0.17028271058350697 1107 52-53 0.3696491475237238 1107 54-55 0.5128559770682415 1107 56-57 0.5349892298490602 1107 58-59 0.5936579822772856 1107 60-61 0.6154355943853247 1107 62-63 0.5098407965869427 1107 64-65 0.5987119974003363 1107 66-67 0.5862953493848551 1107 68-69 0.5285605198947323 1107 70-71 0.3747230570114297 1107 72-73 0.49081997615378015 1107 74-75 0.5291814071964183 1107 76-77 0.49397572834431 1107 78-79 0.49015657571682425 1107 80-81 0.46349143125535974 1107 82-83 0.4718735299995309 1107 84-85 0.466710639240663 1107 86-87 0.3477289303561619 1107 88-89 0.42305474145100064 1107 90-91 0.5019891633122171 1107 92-93 0.5873973928359604 1107 94-95 0.5298929046048357 1107 96-97 0.4080977832531687 1107 98-99 0.622744786687786 1107 100-101 0.5383168846873652 1108 1 0.1950321609727581 1108 2 0.16964802809902224 1108 3 0.15555010136598213 1108 4 0.24132934617062318 1108 5 0.20703994604029674 1108 6 0.20201256360793707 1108 7 0.19066936937916523 1108 8 0.19290595468347504 1108 9 0.19604593916687207 1108 10-11 0.1592897150324717 1108 12-13 0.24044156165011543 1108 14-15 0.43970203442459876 1108 16-17 0.5057327500636148 1108 18-19 0.42798416295492103 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-2.104114279342305 2316 66-67 -1.9814564775144774 2316 68-69 -2.2561471588214204 2316 70-71 -2.019000895977861 2316 72-73 -2.3284977495521986 2316 74-75 -2.445909668213389 2316 76-77 -2.7637796810515702 2316 78-79 -2.953914670748503 2316 80-81 -3.033092258298705 2316 82-83 -2.6407330318820073 2316 84-85 -2.8001872317825054 2316 86-87 -3.254271493978383 2316 88-89 -3.2072438578971934 2316 90-91 -2.705148143693826 2316 92-93 -2.7277635934288185 2316 94-95 -3.417692110125138 2316 96-97 -3.605855101699998 2316 98-99 -3.6760519118503474 2316 100-101 -3.6602009356243457 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4942.0 3 1694.0 4 1884.0 5 1945.0 6 2217.0 7 2595.0 8 3206.0 9 3940.0 10 5026.0 11 6010.0 12 7545.0 13 9148.0 14 10055.0 15 12223.0 16 14875.0 17 18903.0 18 17325.0 19 12665.0 20 12747.0 21 13502.0 22 15049.0 23 17044.0 24 19081.0 25 22346.0 26 26250.0 27 31123.0 28 37760.0 29 45457.0 30 56671.0 31 71174.0 32 91617.0 33 119575.0 34 165225.0 35 235849.0 36 359482.0 37 531966.0 38 467914.0 39 74079.0 40 33.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.35227007473089 18.808887989194247 14.588391663753775 23.250450272321093 2 15.11533753883815 20.101175344443398 40.68476759878229 24.098719517936164 3 19.265216596600258 24.45955773046018 29.2651146152597 27.010111057679865 4 12.959498208869617 16.659022993398455 37.348766099282486 33.03271269844944 5 14.299823735581398 36.88713583661246 33.82699404028497 14.98604638752117 6 31.87402486917158 35.93386533227436 17.846934675259853 14.345175123294208 7 28.751192897862833 32.132234297697075 20.831332090208193 18.285240714231897 8 26.113589416780204 35.05821053424666 19.891058321979518 18.937141726993616 9 26.19672481097736 17.242733792397658 20.298881011092572 36.261660385532416 10-11 24.728457674912892 26.766778818805847 27.56309367231574 20.941669833965516 12-13 25.769822362948926 23.92667926771097 27.996510480038566 22.30698788930153 14-15 22.75809756416276 25.359120542422954 26.015311515107754 25.867470378306535 16-17 22.443997367236214 28.66057652033771 26.43561646791785 22.459809644508226 18-19 22.32784591038589 27.36414003531426 28.5707531911229 21.737260863176946 20-21 22.978347569293017 26.576054042696697 28.596553903113076 21.84904448489721 22-23 22.743694347467933 26.512552465382655 28.491742831365475 22.25201035578394 24-25 22.63373670516309 26.679567662375657 28.482307934269247 22.204387698192004 26-27 22.504385667329338 26.949371770068275 28.28774056458922 22.258501998013163 28-29 22.294227465622868 26.880897845202888 28.553689511542718 22.271185177631526 30-31 22.531237989159848 26.62278706398199 28.473907552808477 22.372067394049683 32-33 22.276853512214586 26.84022749711021 28.46682595255346 22.416093038121744 34-35 22.68408164481853 26.809226651983586 28.283926970509132 22.222764732688752 36-37 22.683196891916083 26.597399877764488 28.194147793594144 22.52525543672528 38-39 22.453720452651005 26.84363751447844 28.384690998099344 22.31795103477121 40-41 22.710170640112192 26.688204529696524 28.289269344075596 22.31235548611569 42-43 22.57389376991167 26.698737103982257 28.187357549689963 22.54001157641611 44-45 22.635802304533463 26.882295780877957 27.959055787528662 22.522846127059918 46-47 22.682890579566074 26.97688253192176 27.71202123241661 22.628205656095552 48-49 22.762437828747622 27.080029967293612 27.551087431990773 22.606444771967993 50-51 22.62708085699798 27.162210864358848 27.535332754764664 22.675375523878508 52-53 22.879447654035538 27.189611277821612 27.351984081543655 22.578956986599195 54-55 22.759603268940943 27.000724331052627 27.41938140481008 22.820290995196356 56-57 22.650018601500555 27.447872162878934 27.331183783021583 22.570925452598924 58-59 22.839186865290003 27.119987764154068 27.34333116983707 22.697494200718857 60-61 22.795644140184823 26.986437358040178 27.70461531461886 22.513303187156144 62-63 22.744869949025993 27.058226926683638 27.68099850533252 22.515904618957844 64-65 22.66017009956823 27.110846695670883 27.508790190204596 22.72019301455629 66-67 22.623907929036736 27.356295511740562 27.30587752881679 22.713919030405908 68-69 22.469763782121937 27.19516079305695 27.610234617517825 22.72484080730329 70-71 22.53681880143057 27.093821438495898 27.52361190289748 22.845747857176054 72-73 22.657568191498626 27.11453849888887 27.356708233025763 22.87118507658674 74-75 22.520625420401664 27.33335425127812 27.29929068556603 22.846729642754187 76-77 22.61005058929319 27.271090557678562 27.30968707987303 22.809171773155217 78-79 22.641764050186797 27.252838340839414 27.214119650951208 22.891277958022584 80-81 22.535556847414465 27.390795426819466 27.22875081689155 22.84489690887452 82-83 22.587484456578448 27.380114619249913 27.310409483350394 22.721991440821242 84-85 22.678493298736466 27.249366703581586 27.14260303703801 22.92953696064394 86-87 22.661910432493322 27.108848180189337 27.378352427303277 22.850888960014057 88-89 22.6414080491497 27.103169381375146 27.217108889889918 23.038313679585237 90-91 22.555073681609553 27.1313835905089 27.44454391333721 22.868998814544337 92-93 22.718165883210624 27.10453906326155 27.343586810636644 22.83370824289118 94-95 22.57804817682644 27.100357209677412 27.2080653214789 23.113529292017244 96-97 22.593441468583798 27.13539659235678 27.191160879871408 23.080001059188014 98-99 22.448000696829112 27.117069645244925 27.158169222753042 23.276760435172918 100-101 22.479320228476627 27.09158469019043 27.224371572541607 23.204723508791343 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 342.0 1 238.5 2 183.0 3 243.0 4 239.0 5 260.5 6 425.0 7 854.0 8 1481.0 9 1845.5 10 1739.5 11 1592.5 12 1646.5 13 1858.5 14 2065.0 15 2194.0 16 2394.0 17 2541.5 18 2697.5 19 2916.5 20 3236.0 21 3781.0 22 4479.5 23 5508.5 24 6789.5 25 8614.0 26 11401.5 27 14754.5 28 17957.0 29 21560.0 30 26347.0 31 31279.0 32 36193.0 33 41711.5 34 48410.0 35 53959.5 36 59537.5 37 65788.5 38 69540.5 39 72300.5 40 75131.5 41 79652.0 42 84846.0 43 89394.0 44 94436.0 45 98563.0 46 99648.0 47 100635.0 48 104126.0 49 104407.0 50 101301.5 51 97884.5 52 93122.0 53 90010.0 54 86831.5 55 80721.0 56 74295.5 57 66736.5 58 59510.0 59 52537.0 60 44091.0 61 35207.0 62 27200.5 63 20863.5 64 15940.0 65 12055.0 66 8856.0 67 6759.5 68 4921.5 69 3288.0 70 2227.0 71 1486.0 72 964.0 73 625.5 74 392.5 75 257.0 76 167.5 77 93.0 78 52.0 79 27.5 80 21.5 81 15.5 82 13.5 83 15.5 84 10.0 85 8.5 86 10.0 87 9.0 88 9.0 89 5.0 90 3.0 91 4.5 92 4.5 93 5.0 94 3.5 95 3.0 96 2.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044272044458700734 2 0.0432132798879435 3 0.025724057719138778 4 0.046781708626421586 5 0.06658452744984397 6 0.06925104562804738 7 0.06768250552322184 8 0.07081958573287292 9 0.06940789963852993 10-11 0.07711335290348537 12-13 0.07609380183534878 14-15 0.07052548446321813 16-17 0.05840851215344087 18-19 0.051506935692208516 20-21 0.0584477256560615 22-23 0.033017769206577514 24-25 0.018351919226458763 26-27 0.014077647440809178 28-29 0.0189009082631477 30-31 0.014195287948671092 32-33 0.008607363825230124 34-35 0.010509218702331086 36-37 0.005744778133923523 38-39 0.009038712354057146 40-41 0.008391689560816613 42-43 0.0050977553406829895 44-45 0.0014901130995842585 46-47 0.0030978667070304324 48-49 0.0011175848246881938 50-51 0.006705508948129163 52-53 0.0057251713826132035 54-55 0.00796034103198959 56-57 0.024175124365623562 58-59 0.010077870173504064 60-61 0.022155628980660685 62-63 0.016116749577082375 64-65 0.011077814490330342 66-67 0.01790096394632142 68-69 0.02807686787637708 70-71 0.020685122632386744 72-73 0.018979335268388978 74-75 0.019489110802457275 76-77 0.02703771005693016 78-79 0.038644906832639124 80-81 0.03309619621181879 82-83 0.032625634180371135 84-85 0.02295950578438377 86-87 0.025331922692932392 88-89 0.02131253867431696 90-91 0.01919500953280249 92-93 0.033743219005059326 94-95 0.025841698227000696 96-97 0.03997816592174083 98-99 0.057016432810408205 100-101 0.06656492069853365 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2550142.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.14918538164468 #Duplication Level Percentage of deduplicated Percentage of total 1 78.39710418032296 45.5872774436571 2 12.409610754455219 14.43217512549736 3 3.857186639203827 6.72876782803999 4 1.7306002911057372 4.02531988596143 5 0.9230324463933497 2.683679241929995 6 0.5716523837730207 1.994467226272588 7 0.3788367413415054 1.5420333531151762 8 0.27221382330160326 1.2663209659688954 9 0.20423333639795993 1.0688401925385096 >10 1.1300425284302311 12.08870082287615 >50 0.0837648518679019 3.322889565916614 >100 0.040370324805438396 4.139436589353599 >500 0.0010137739509746971 0.38675214901693356 >1k 2.0275479019259146E-4 0.2315561083210163 >5k 1.3516986012839432E-4 0.5017835015347482 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7616 0.2986500359587819 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5152 0.20202796550152893 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3439 0.13485523551237538 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 3.921350262063838E-5 0.0 3 0.0 0.0 0.0 3.921350262063838E-5 0.0 4 0.0 0.0 0.0 3.921350262063838E-5 0.0 5 0.0 0.0 0.0 3.921350262063838E-5 0.0 6 0.0 0.0 0.0 3.921350262063838E-5 0.0 7 0.0 0.0 0.0 3.921350262063838E-5 3.921350262063838E-5 8 0.0 0.0 0.0 3.921350262063838E-5 3.921350262063838E-5 9 0.0 0.0 0.0 3.921350262063838E-5 3.921350262063838E-5 10-11 0.0 0.0 0.0 3.921350262063838E-5 3.921350262063838E-5 12-13 0.0 0.0 0.0 7.842700524127676E-5 3.921350262063838E-5 14-15 0.0 0.0 0.0 1.3724725917223433E-4 3.921350262063838E-5 16-17 0.0 0.0 0.0 1.960675131031919E-4 3.921350262063838E-5 18-19 0.0 0.0 0.0 1.960675131031919E-4 3.921350262063838E-5 20-21 0.0 0.0 0.0 2.7449451834446866E-4 3.921350262063838E-5 22-23 0.0 0.0 0.0 3.921350262063838E-4 3.921350262063838E-5 24-25 3.921350262063838E-5 0.0 0.0 6.078092906198949E-4 3.921350262063838E-5 26-27 3.921350262063838E-5 0.0 0.0 9.999443168262788E-4 1.3724725917223433E-4 28-29 3.921350262063838E-5 0.0 0.0 0.0019018548771009614 1.5685401048255352E-4 30-31 3.921350262063838E-5 0.0 0.0 0.003960563764684477 1.764607617928727E-4 32-33 3.921350262063838E-5 0.0 0.0 0.007430958746610973 1.960675131031919E-4 34-35 3.921350262063838E-5 0.0 0.0 0.010117083676124702 1.960675131031919E-4 36-37 3.921350262063838E-5 0.0 0.0 0.01162680352701928 2.156742644135111E-4 38-39 3.921350262063838E-5 0.0 0.0 0.013097309875293218 2.548877670341495E-4 40-41 3.921350262063838E-5 0.0 0.0 0.015626580794324395 2.7449451834446866E-4 42-43 3.921350262063838E-5 0.0 0.0 0.01866562724742387 2.941012696547878E-4 44-45 3.921350262063838E-5 0.0 0.0 0.021763493954454302 3.1370802096510704E-4 46-47 3.921350262063838E-5 0.0 0.0 0.025351529444242713 3.3331477227542625E-4 48-49 3.921350262063838E-5 0.0 0.0 0.030115970012650276 3.529215235857454E-4 50-51 3.921350262063838E-5 0.0 0.0 0.03721361398698582 3.529215235857454E-4 52-53 3.921350262063838E-5 0.0 0.0 0.05470283615579054 3.725282748960646E-4 54-55 3.921350262063838E-5 0.0 0.0 0.07656436386679644 3.921350262063838E-4 56-57 3.921350262063838E-5 0.0 0.0 0.10358246717241629 3.921350262063838E-4 58-59 3.921350262063838E-5 0.0 0.0 0.1355806853108572 3.921350262063838E-4 60-61 3.921350262063838E-5 0.0 0.0 0.17963705550514442 3.921350262063838E-4 62-63 3.921350262063838E-5 0.0 0.0 0.21298813948399736 3.921350262063838E-4 64-65 3.921350262063838E-5 0.0 0.0 0.24392759305168105 4.11741777516703E-4 66-67 3.921350262063838E-5 0.0 0.0 0.3043752073413951 4.7056203144766055E-4 68-69 3.921350262063838E-5 0.0 0.0 0.386311821067219 5.293822853786182E-4 70-71 3.921350262063838E-5 0.0 0.0 0.4538374725799583 6.274160419302141E-4 72-73 3.921350262063838E-5 0.0 0.0 0.5192063814485626 7.842700524127676E-4 74-75 3.921350262063838E-5 0.0 0.0 0.6355528437239966 7.842700524127676E-4 76-77 3.921350262063838E-5 0.0 0.0 0.7244498541649838 7.842700524127676E-4 78-79 3.921350262063838E-5 0.0 0.0 0.794798877866409 7.842700524127676E-4 80-81 3.921350262063838E-5 0.0 0.0 0.8865192604960822 8.23483555033406E-4 82-83 3.921350262063838E-5 0.0 0.0 1.0421772591487062 8.626970576540444E-4 84-85 7.842700524127676E-5 0.0 0.0 1.2058348123359406 8.626970576540444E-4 86-87 7.842700524127676E-5 0.0 0.0 1.4104900825130522 9.019105602746828E-4 88-89 7.842700524127676E-5 0.0 0.0 1.6742597078907764 9.019105602746828E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4255 0.0 44.121395 1 GTATCAA 7870 0.0 43.482 1 ATCAACG 9885 0.0 34.23312 3 TCAACGC 9935 0.0 34.108677 4 TATCAAC 10060 0.0 33.543785 2 CAACGCA 10190 0.0 33.163795 5 AACGCAG 10405 0.0 32.567326 6 ACGCAGA 12215 0.0 27.432976 7 CGCAGAG 12200 0.0 27.03868 8 GTACATG 9170 0.0 25.50043 1 GCAGAGT 13040 0.0 24.713709 9 TACATGG 9485 0.0 24.202572 2 ACATGGG 9415 0.0 23.271467 3 CATGGGG 5340 0.0 21.627594 4 AGAGTAC 11550 0.0 21.027437 10-11 GAGTACT 6765 0.0 20.548492 12-13 AGTACTT 7280 0.0 19.584467 12-13 GTACTTT 7120 0.0 19.45663 14-15 CAGAGTA 12550 0.0 19.162592 10-11 GTACGGG 275 2.8232535E-8 19.01055 6 >>END_MODULE