FastQCFastQC Report
Thu 26 May 2016
SRR936850_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936850_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2550142
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82080.3218644295101998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61840.24249630020602772No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41260.16179491181275396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46100.045.8699381
GTATCAA83450.041.796391
ATCAACG103550.033.255973
TATCAAC106050.032.698512
TCAACGC105350.032.6388324
CAACGCA107400.031.9716175
AACGCAG110350.031.1587336
ACGCAGA129650.026.373857
CGCAGAG130450.026.0664868
GCAGAGT139950.023.5844359
GTACATG90850.022.0727581
GAGTACT80150.021.42057612-13
TACATGG94000.020.7696762
GTACTTT82150.020.72624814-15
ACATGGG93050.020.6227553
AGTACTT82800.020.16142812-13
AGAGTAC127450.020.14046110-11
CAGAGTA136350.018.98256710-11
CATGGGG54250.018.209344
TGGTATC25850.017.6411322
Produced by FastQC (version 0.11.5)