##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936850_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2550142 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16915214917444 33.0 31.0 34.0 31.0 34.0 2 32.25049899182085 33.0 31.0 34.0 30.0 34.0 3 32.397363754645816 34.0 31.0 34.0 31.0 34.0 4 36.07209010321778 37.0 35.0 37.0 35.0 37.0 5 35.97900509069691 37.0 35.0 37.0 35.0 37.0 6 36.00846423454067 37.0 35.0 37.0 35.0 37.0 7 35.963310278408024 37.0 35.0 37.0 35.0 37.0 8 35.94375254397598 37.0 35.0 37.0 35.0 37.0 9 37.64220384590348 39.0 37.0 39.0 35.0 39.0 10-11 37.53150001058765 39.0 37.0 39.0 35.0 39.0 12-13 37.45311712053682 39.0 37.0 39.0 35.0 39.0 14-15 38.691513649043856 40.0 38.0 41.0 34.5 41.0 16-17 38.605093755563416 40.0 38.0 41.0 34.0 41.0 18-19 38.61830301998869 40.0 38.0 41.0 34.0 41.0 20-21 38.70754314857761 40.0 38.0 41.0 34.0 41.0 22-23 38.6476447193921 40.0 38.0 41.0 34.0 41.0 24-25 38.56055309861176 40.0 38.0 41.0 34.0 41.0 26-27 38.40005595766824 40.0 38.0 41.0 34.0 41.0 28-29 38.24955786775796 40.0 38.0 41.0 34.0 41.0 30-31 38.23778636640626 40.0 38.0 41.0 34.0 41.0 32-33 38.18563613320356 40.0 38.0 41.0 34.0 41.0 34-35 38.08592521514488 40.0 38.0 41.0 33.0 41.0 36-37 37.934905977784766 40.0 37.0 41.0 33.0 41.0 38-39 37.80715681714979 40.0 37.0 41.0 33.0 41.0 40-41 37.59710537687705 40.0 37.0 41.0 33.0 41.0 42-43 37.39689221227681 40.0 37.0 41.0 32.0 41.0 44-45 37.192166161727464 40.0 36.5 41.0 31.5 41.0 46-47 36.89222031557458 39.5 36.0 41.0 31.0 41.0 48-49 36.578647581193515 39.0 36.0 41.0 30.5 41.0 50-51 36.36326682984712 39.0 36.0 40.5 30.0 41.0 52-53 36.14020121232465 39.0 35.0 40.0 30.0 41.0 54-55 35.870521523899455 39.0 35.0 40.0 29.0 41.0 56-57 35.61914650243006 38.0 35.0 40.0 28.5 41.0 58-59 35.263420037001865 38.0 34.0 40.0 28.0 41.0 60-61 34.9340744554617 38.0 34.0 40.0 27.5 41.0 62-63 34.59037163420703 37.0 34.0 40.0 26.5 41.0 64-65 34.174701840132826 36.5 33.5 39.0 26.0 41.0 66-67 33.73385207568833 36.0 33.0 39.0 26.0 40.0 68-69 33.28972484669481 35.5 32.0 39.0 25.0 40.0 70-71 32.88290573622959 35.0 32.0 38.0 25.0 40.0 72-73 32.48025364862035 35.0 32.0 37.5 23.5 39.5 74-75 31.99157301828682 35.0 31.0 37.0 22.5 39.0 76-77 30.545730590688677 33.5 29.5 35.5 20.5 37.5 78-79 31.26177032494661 34.0 31.0 36.0 21.5 38.0 80-81 30.97358323575707 34.0 31.0 35.5 20.0 37.0 82-83 30.760697443514907 34.0 31.0 35.0 20.0 37.0 84-85 30.4193835088399 34.0 30.5 35.0 18.5 36.5 86-87 30.038791761399956 34.0 30.0 35.0 17.5 36.0 88-89 29.660743793875007 34.0 30.0 35.0 12.0 36.0 90-91 29.197883098274527 34.0 29.0 35.0 7.0 35.0 92-93 28.738541030264194 34.0 29.0 35.0 2.0 35.0 94-95 28.2198399147969 33.0 29.0 35.0 2.0 35.0 96-97 27.562382408509016 33.0 27.0 35.0 2.0 35.0 98-99 26.711963686728033 33.0 25.5 34.0 2.0 35.0 100-101 23.31796366633701 29.0 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2704463498065408 1101 2 0.2591603038716599 1101 3 0.24183636007243337 1101 4 0.16989652150710555 1101 5 0.19601090589409864 1101 6 0.16575542640368468 1101 7 0.10744681360843344 1101 8 0.16304248558564183 1101 9 0.29222292963412855 1101 10-11 0.24071543009271323 1101 12-13 0.2572923946259493 1101 14-15 0.3372288630311999 1101 16-17 0.35096038068148516 1101 18-19 0.34202807190516893 1101 20-21 0.279675097267301 1101 22-23 0.25504698460184727 1101 24-25 0.351052341215528 1101 26-27 0.22736139014921264 1101 28-29 0.24339687801964516 1101 30-31 0.15111727383510498 1101 32-33 0.30584799326446444 1101 34-35 0.32932585383195345 1101 36-37 0.33263861457854915 1101 38-39 0.34034726386389025 1101 40-41 0.39753603181853947 1101 42-43 0.39528731443757437 1101 44-45 0.2964598376918133 1101 46-47 0.36560796297571585 1101 48-49 0.43661121889485344 1101 50-51 0.3071589155498131 1101 52-53 0.2846514352637257 1101 54-55 0.23204386503686436 1101 56-57 0.37400605245449725 1101 58-59 0.36960457278323844 1101 60-61 0.24353252505049028 1101 62-63 0.21552847473849823 1101 64-65 0.3367412933832412 1101 66-67 0.3299684922327941 1101 68-69 0.3284852932353104 1101 70-71 0.4134038156898896 1101 72-73 0.3734658491492908 1101 74-75 0.3474690435383394 1101 76-77 0.18460261532060684 1101 78-79 0.41286794402991944 1101 80-81 0.7676697650612674 1101 82-83 0.6210926375711558 1101 84-85 0.45437485043197157 1101 86-87 0.24841613234906035 1101 88-89 0.11116620813016809 1101 90-91 0.09602099579424106 1101 92-93 0.44381824726386654 1101 94-95 0.45148450193189404 1101 96-97 0.379242300139353 1101 98-99 0.5091279207933894 1101 100-101 0.80517991672777 1102 1 0.24315696187181857 1102 2 0.15124896965026124 1102 3 0.11863780628259946 1102 4 0.1056202374795916 1102 5 0.11287541028361403 1102 6 0.20482089374711876 1102 7 0.2302753928658987 1102 8 0.1646568983415193 1102 9 0.1776758963696352 1102 10-11 0.2514590445030933 1102 12-13 0.29873654080800094 1102 14-15 0.24633878900070272 1102 16-17 0.3592899676124546 1102 18-19 0.3589611977332723 1102 20-21 0.2251381941166457 1102 22-23 0.2873390948563852 1102 24-25 0.2633783410897834 1102 26-27 0.3074472944635218 1102 28-29 0.31932362329947495 1102 30-31 0.40546883386077326 1102 32-33 0.3938200844449753 1102 34-35 0.37054918962861905 1102 36-37 0.38486403689397974 1102 38-39 0.3216137890569115 1102 40-41 0.4596948373666194 1102 42-43 0.46059025110301377 1102 44-45 0.5390110012907741 1102 46-47 0.5500477403979005 1102 48-49 0.6717350162403832 1102 50-51 0.50667287173885 1102 52-53 0.4207276293775877 1102 54-55 0.503910320718802 1102 56-57 0.5526925420379172 1102 58-59 0.5923835097381129 1102 60-61 0.5091262576653008 1102 62-63 0.3715228569142468 1102 64-65 0.30543182789502765 1102 66-67 0.17914805976946013 1102 68-69 0.26470786393555557 1102 70-71 0.2750857111362066 1102 72-73 0.43664252948489235 1102 74-75 0.41082922839513003 1102 76-77 0.5009263922635654 1102 78-79 0.5216538012522491 1102 80-81 0.5625864407232548 1102 82-83 0.6129593067683707 1102 84-85 0.392224526185295 1102 86-87 0.43730017101430363 1102 88-89 0.37785266450010013 1102 90-91 0.2800800636102494 1102 92-93 0.4397010202579814 1102 94-95 0.5635569524111759 1102 96-97 0.6846798819127429 1102 98-99 0.8156609545726958 1102 100-101 1.3677561446482152 1103 1 0.1849771824589439 1103 2 0.1016207846560917 1103 3 0.18006339354533196 1103 4 0.14584076432050352 1103 5 0.20790873346877703 1103 6 0.22041933956943893 1103 7 0.1785034313646463 1103 8 0.11885989999034763 1103 9 0.18512184227484596 1103 10-11 0.09023330592204815 1103 12-13 0.22068101120899541 1103 14-15 0.2503842930082243 1103 16-17 0.25933397652275403 1103 18-19 0.2586697140602823 1103 20-21 0.25844871917912116 1103 22-23 0.286628622242894 1103 24-25 0.12975282074470584 1103 26-27 0.1881285885666344 1103 28-29 0.2120298999949526 1103 30-31 0.29205244626887605 1103 32-33 0.23669802603370016 1103 34-35 0.29238152773762494 1103 36-37 0.37249873882404216 1103 38-39 0.17978392268122434 1103 40-41 0.34040205452647143 1103 42-43 0.22346752954377536 1103 44-45 0.2884944994565686 1103 46-47 0.3267305537127072 1103 48-49 0.22976619022328038 1103 50-51 0.3733424522374804 1103 52-53 0.4001606981550694 1103 54-55 0.39844357005339504 1103 56-57 0.2966610605955111 1103 58-59 0.26702171730945423 1103 60-61 0.1855826963798819 1103 62-63 0.2896448821681261 1103 64-65 0.18324892565810558 1103 66-67 0.14313460389442412 1103 68-69 0.18742882031548902 1103 70-71 0.1681338104637078 1103 72-73 0.283351657716608 1103 74-75 0.1545463535884224 1103 76-77 0.23909568188138053 1103 78-79 0.29411989781262093 1103 80-81 0.5790593375645585 1103 82-83 0.3467183609988034 1103 84-85 0.2960204454138733 1103 86-87 0.3205372976544645 1103 88-89 0.40771783446190923 1103 90-91 0.3137670758573883 1103 92-93 0.3607062319981118 1103 94-95 0.5706541427738259 1103 96-97 0.7143946378592183 1103 98-99 0.8620773934078443 1103 100-101 0.7389399541921193 1104 1 0.14317126882976794 1104 2 0.06404676030911816 1104 3 0.09090571924426882 1104 4 0.10463088966010048 1104 5 0.023234081383989746 1104 6 0.14024535517685877 1104 7 0.14505101207537052 1104 8 0.16761800509310376 1104 9 0.12134289975561785 1104 10-11 0.16744511906401982 1104 12-13 0.19028806536611143 1104 14-15 0.22836318019628976 1104 16-17 0.2643841117312107 1104 18-19 0.21416914719936386 1104 20-21 0.14531664090126384 1104 22-23 0.12329031246522248 1104 24-25 0.18423398388406298 1104 26-27 0.23127228134881506 1104 28-29 0.19544617756837113 1104 30-31 0.2757870986435549 1104 32-33 0.23049141893731928 1104 34-35 0.1976578401356477 1104 36-37 0.25983943326175307 1104 38-39 0.34061733111286685 1104 40-41 0.30994174077731174 1104 42-43 0.22547577376219863 1104 44-45 0.32954499765767054 1104 46-47 0.3255936809069624 1104 48-49 0.4449164067899929 1104 50-51 0.37926767701248565 1104 52-53 0.3838399563392656 1104 54-55 0.41334857752435994 1104 56-57 0.31896670670667504 1104 58-59 0.34798338314320887 1104 60-61 0.30774932134860933 1104 62-63 0.3595691767744711 1104 64-65 0.4557099464867065 1104 66-67 0.18188198937055944 1104 68-69 0.3282012762317379 1104 70-71 0.40274452920805714 1104 72-73 0.32716915560072835 1104 74-75 0.4492989041474402 1104 76-77 0.6512296564176978 1104 78-79 0.7286507707784295 1104 80-81 0.8468167047083028 1104 82-83 0.6598549136651926 1104 84-85 0.47647429965845944 1104 86-87 0.4241867601921747 1104 88-89 0.5905867241644494 1104 90-91 0.6730791921864494 1104 92-93 0.6595009829731815 1104 94-95 0.6426002938228805 1104 96-97 0.811000782644129 1104 98-99 1.2053794543364127 1104 100-101 1.3216816704289194 1105 1 0.1722312949182907 1105 2 0.09165523757911842 1105 3 0.06994213072321287 1105 4 0.04063529772055574 1105 5 0.12245479926812664 1105 6 0.14072701824510858 1105 7 0.1469008231584965 1105 8 0.12179348038397109 1105 9 0.04661952803917302 1105 10-11 0.08310347938547835 1105 12-13 0.11597513805413229 1105 14-15 0.19308604588538714 1105 16-17 0.14650176121543268 1105 18-19 0.14667634403276253 1105 20-21 0.23391078447809122 1105 22-23 -0.006304228436782466 1105 24-25 0.06072442184136406 1105 26-27 0.1840201035570388 1105 28-29 0.13819105732127213 1105 30-31 0.22146272012010115 1105 32-33 0.07604765544325431 1105 34-35 0.20837952884262734 1105 36-37 0.1793674971636321 1105 38-39 0.3650917186323568 1105 40-41 0.34318773753873444 1105 42-43 0.29901044975476765 1105 44-45 0.3342473036430462 1105 46-47 0.2836168382177533 1105 48-49 0.46191986276935637 1105 50-51 0.2799588728863043 1105 52-53 0.36928839027833504 1105 54-55 0.2366049384755442 1105 56-57 0.09535650144698593 1105 58-59 0.2335701312240559 1105 60-61 0.2610603332533685 1105 62-63 0.27752902444149896 1105 64-65 0.2702303075278394 1105 66-67 0.27361162832941943 1105 68-69 0.3041195284507552 1105 70-71 0.2795020371545647 1105 72-73 0.34810041459677166 1105 74-75 0.1828328679533655 1105 76-77 0.149570074133905 1105 78-79 0.25067748527404987 1105 80-81 0.5090078406085077 1105 82-83 0.33999736973452244 1105 84-85 0.09859509775729691 1105 86-87 0.15149893033730777 1105 88-89 -0.0012431831763208834 1105 90-91 -0.058120954748901 1105 92-93 0.0408614656108206 1105 94-95 0.14629101365615327 1105 96-97 0.2527069007021865 1105 98-99 0.5359891423236967 1105 100-101 0.5666799649293388 1106 1 0.14772143385898318 1106 2 0.10444392028457372 1106 3 0.11851017902608874 1106 4 0.08531312315881934 1106 5 0.03654352548968376 1106 6 0.1597594843647201 1106 7 0.08151593878699259 1106 8 0.11785274775932919 1106 9 0.16400809573779185 1106 10-11 0.20534875478023906 1106 12-13 0.18942604256736928 1106 14-15 0.36096154709761663 1106 16-17 0.2845502777826425 1106 18-19 0.2261942478000094 1106 20-21 0.21638584042737108 1106 22-23 0.29654937873716136 1106 24-25 0.2277334864006093 1106 26-27 0.34068340938198816 1106 28-29 0.30738418333147166 1106 30-31 0.15621819460101705 1106 32-33 0.21676153319409508 1106 34-35 0.10265823004242236 1106 36-37 0.25145220980195404 1106 38-39 0.26518681296904845 1106 40-41 0.2236253074753236 1106 42-43 0.13943603333675725 1106 44-45 0.24155995128695196 1106 46-47 0.2523095290669346 1106 48-49 0.31704718621831773 1106 50-51 0.10941461079504222 1106 52-53 0.2131486375430356 1106 54-55 0.2664683484282975 1106 56-57 0.17885253448814353 1106 58-59 0.41421585965250074 1106 60-61 0.3368429431315221 1106 62-63 0.41922444281772897 1106 64-65 0.3028401856827614 1106 66-67 0.30379315263627404 1106 68-69 0.5116791882480953 1106 70-71 0.5262242451304431 1106 72-73 0.447910128789232 1106 74-75 0.36747955218189077 1106 76-77 0.3368534649706554 1106 78-79 0.4106841486770101 1106 80-81 0.831081382819125 1106 82-83 0.6630718794013113 1106 84-85 0.7175625811279289 1106 86-87 0.5111293931621255 1106 88-89 0.44169730078488456 1106 90-91 0.4908560283419874 1106 92-93 0.6534142094564004 1106 94-95 0.8021529627112329 1106 96-97 0.8283962617925731 1106 98-99 0.9009294436726982 1106 100-101 0.6742273858232828 1107 1 0.08787216165761436 1107 2 0.06646976564192641 1107 3 0.029174994644741048 1107 4 0.057796297773720084 1107 5 0.016796437579571943 1107 6 -0.009199823071988078 1107 7 0.02189168524579088 1107 8 0.0935322701034309 1107 9 0.0286344544042052 1107 10-11 0.034017381418195214 1107 12-13 0.1031579694114555 1107 14-15 0.19285074463333274 1107 16-17 0.2059471369885557 1107 18-19 0.2353406802547866 1107 20-21 0.13805324952142684 1107 22-23 0.1266082347900408 1107 24-25 0.18153101985738118 1107 26-27 0.22889300072949226 1107 28-29 0.1615897904189083 1107 30-31 0.07201721143188422 1107 32-33 0.2069559390441924 1107 34-35 0.17555275431535478 1107 36-37 0.2119720542683865 1107 38-39 0.21833510998312988 1107 40-41 0.38558516031506684 1107 42-43 0.2711610685852932 1107 44-45 0.17831159245867667 1107 46-47 0.23994439971313852 1107 48-49 0.41428821590704246 1107 50-51 0.37173785280726435 1107 52-53 0.24498031025901668 1107 54-55 0.10935541973894658 1107 56-57 0.2003781859907292 1107 58-59 0.3254841722638062 1107 60-61 0.3327639420089312 1107 62-63 0.44140724320201485 1107 64-65 0.3055552627797624 1107 66-67 0.22504002253786837 1107 68-69 0.2805120771198375 1107 70-71 0.16641586774541395 1107 72-73 0.17887769375833784 1107 74-75 0.1685597944468853 1107 76-77 0.3729262744015074 1107 78-79 0.32306186852586194 1107 80-81 0.5086345707199804 1107 82-83 0.49354953946266633 1107 84-85 0.42218762383080843 1107 86-87 0.33913087625859006 1107 88-89 0.1627487056800767 1107 90-91 0.1901541950021084 1107 92-93 0.12115568147822486 1107 94-95 0.32393819773912824 1107 96-97 0.29225834825831853 1107 98-99 0.41418069678787006 1107 100-101 0.5675484127690247 1108 1 0.13206744738719323 1108 2 0.0786255699073024 1108 3 0.08051683388944042 1108 4 0.1016629079731004 1108 5 -0.02139767905829615 1108 6 0.04993554004433065 1108 7 0.008761217052196457 1108 8 0.017449145354447637 1108 9 -0.0029116630652410436 1108 10-11 0.11917506501181663 1108 12-13 0.06343438230592113 1108 14-15 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-1.118143823762992 2316 56-57 -1.2661341666660562 2316 58-59 -1.4482431893144465 2316 60-61 -1.170087794806264 2316 62-63 -1.3417494579795104 2316 64-65 -1.1914758562460719 2316 66-67 -1.1563527686443251 2316 68-69 -1.4041540724615658 2316 70-71 -1.0901102931747673 2316 72-73 -0.9994206551629148 2316 74-75 -1.129904601450285 2316 76-77 -1.2307830355679208 2316 78-79 -1.0476556394478038 2316 80-81 -1.0253991464853947 2316 82-83 -1.0053368953967627 2316 84-85 -1.4070458262524959 2316 86-87 -1.2022687321014978 2316 88-89 -1.0579158300388052 2316 90-91 -1.028401606729318 2316 92-93 -1.0098834859913133 2316 94-95 -1.0804776640251958 2316 96-97 -1.1805373555283438 2316 98-99 -1.3510728431013135 2316 100-101 -1.5754375111498753 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 15.0 5 43.0 6 88.0 7 250.0 8 627.0 9 1097.0 10 1884.0 11 2928.0 12 4665.0 13 6323.0 14 8153.0 15 11099.0 16 14810.0 17 19578.0 18 18116.0 19 12209.0 20 11312.0 21 11956.0 22 12795.0 23 14277.0 24 16729.0 25 20496.0 26 25206.0 27 31276.0 28 39490.0 29 49711.0 30 62315.0 31 78547.0 32 101124.0 33 131941.0 34 181046.0 35 257235.0 36 397040.0 37 583322.0 38 398004.0 39 24428.0 40 1.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.31595058334746 19.52954863529958 15.156639303623093 22.997861477729874 2 15.616990070432477 20.66146135625657 39.83329150261181 23.888257070699147 3 19.557701342716165 24.477843496975968 29.643602548486104 26.32085261182177 4 13.603723717129256 17.265208968919353 36.7856666692809 32.34540064467049 5 14.407202385527466 37.017871064185556 33.81561259335133 14.759313956935646 6 32.13970045589618 36.18061268745035 17.460204176865446 14.219482679788028 7 28.826512405975823 32.22424476754628 20.687514656046606 18.261728170431294 8 26.35745488482985 35.100700353706074 19.709662998894178 18.8321817625699 9 26.154200885362812 17.035528623711393 20.22958730517254 36.58068318575326 10-11 24.78499236513104 26.829623605273746 27.45321633069845 20.932167698896766 12-13 25.850179813868824 23.912970165916576 28.055747593168523 22.181102427046078 14-15 22.78363322513021 25.298434361694365 26.140799218239614 25.777133194935814 16-17 22.372316348844308 28.665930367366034 26.585731256768007 22.37602202702165 18-19 22.267945139296838 27.166942089127073 28.911240765765946 21.653872005810147 20-21 22.8541424275257 26.399144978395057 29.06910718788319 21.677605406196044 22-23 22.708813862130032 26.322181274611374 28.925467679839006 22.04353718341959 24-25 22.60405077528029 26.496883702270722 28.942392119784422 21.95667340266457 26-27 22.529834536022033 26.759140558302196 28.699253053258044 22.011771852417723 28-29 22.26088794602651 26.675774147291374 28.942081874381476 22.12125603230064 30-31 22.551086914096786 26.432705824066094 28.927215502076386 22.08899175976073 32-33 22.322127941496237 26.612601374872973 28.837765499084067 22.22750518454673 34-35 22.685324799031225 26.56691723957 28.706192061797093 22.04156589960169 36-37 22.672965175565476 26.38092500149037 28.646776253243512 22.29933356970064 38-39 22.512739545415688 26.561511003814214 28.841883750716686 22.083865700053412 40-41 22.68855426695259 26.497955194683975 28.732828576762167 22.08066196160127 42-43 22.581126505328367 26.495543990774845 28.543223907135086 22.380105596761705 44-45 22.651044116554242 26.68657327462394 28.33195657617062 22.3304260326512 46-47 22.730533742128 26.790769062449943 28.03150544969145 22.447191745730606 48-49 22.84173060358899 26.958522680148988 27.74021613624069 22.45953058002133 50-51 22.726275429424064 27.009683058030703 27.71470222905427 22.54933928349096 52-53 22.96086019380203 27.12355884225434 27.501900433183085 22.413680530760548 54-55 22.839125650660247 26.890316544200978 27.622816655961213 22.647741149177563 56-57 22.801864377734912 27.19023405500981 27.611369943536147 22.39653162371913 58-59 22.972413645713466 26.923777493564515 27.57508030506021 22.528728555661804 60-61 22.88604826164514 26.83090856544795 27.95212107879525 22.330922094111656 62-63 22.802046711562248 26.88196077116566 27.932589570099985 22.383402947172108 64-65 22.755575912965796 26.942030269970108 27.676191163402464 22.62620265366163 66-67 22.692599759266844 27.190553096091506 27.57253414683546 22.544312997806188 68-69 22.617726253435375 26.986674948624994 27.791930698203988 22.603668099735643 70-71 22.619192107145988 26.975179148202184 27.707693191535 22.697935553116828 72-73 22.741180673779933 26.9498835828938 27.565813804005913 22.743121939320353 74-75 22.66775868874214 27.142938094936508 27.435133084798313 22.75417013152304 76-77 22.696724342592095 27.092554546905497 27.478703383812025 22.732017726690387 78-79 22.702400094121337 27.03655045295894 27.445958665045687 22.815090787874034 80-81 22.75541558927399 27.16053013601617 27.402144511395072 22.68190976331477 82-83 22.62946183913703 27.186020542915713 27.50125243747371 22.683265180473544 84-85 22.687873511834738 27.143977538311926 27.36582984330149 22.80231910655185 86-87 22.630955452676755 26.947440573897453 27.535211764678202 22.88639220874759 88-89 22.699327321024807 26.938589123101742 27.348906043758248 23.013177512115202 90-91 22.617704080272176 26.99874594333452 27.555934534948108 22.8276154414452 92-93 22.674187312791812 26.95229331931578 27.48830405286307 22.88521531502934 94-95 22.532121942209915 26.909160566968065 27.418802051349623 23.139915439472404 96-97 22.63568399320036 26.8687540071408 27.391412611489198 23.104149388169645 98-99 22.410072880469638 26.883542809212614 27.275698595505453 23.430685714812295 100-101 22.198787627344323 27.117353336024706 27.317284829538647 23.366574207092324 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 396.0 1 305.0 2 235.5 3 271.0 4 245.5 5 233.0 6 411.0 7 887.5 8 1565.5 9 1976.5 10 1847.5 11 1629.0 12 1714.5 13 1938.0 14 2207.0 15 2429.0 16 2550.0 17 2749.5 18 3029.5 19 3258.5 20 3569.5 21 4076.0 22 4743.5 23 5756.5 24 7121.5 25 8960.5 26 11614.0 27 14892.5 28 17944.5 29 21528.0 30 26458.0 31 31318.5 32 36061.0 33 41372.0 34 47961.5 35 53757.5 36 59415.5 37 65532.5 38 69342.5 39 71682.0 40 74875.0 41 80136.5 42 85145.5 43 89834.0 44 94945.5 45 98632.5 46 99910.0 47 101343.5 48 104598.0 49 104648.0 50 101351.5 51 97702.0 52 93326.0 53 90133.0 54 86783.5 55 80687.0 56 73299.0 57 65660.0 58 59169.0 59 52291.5 60 43704.0 61 34945.0 62 27260.0 63 20972.0 64 15597.5 65 11644.0 66 8628.5 67 6618.0 68 4804.0 69 3078.0 70 2013.5 71 1268.5 72 847.5 73 564.0 74 347.0 75 226.0 76 154.5 77 85.0 78 40.0 79 30.0 80 19.5 81 6.5 82 6.0 83 6.0 84 4.5 85 2.5 86 2.0 87 2.0 88 2.5 89 1.5 90 1.0 91 0.5 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028155294881618356 2 0.0071368574769561845 3 0.009254386618470656 4 7.842700524127676E-5 5 0.003842923256822561 6 0.0 7 0.0 8 7.842700524127676E-5 9 3.921350262063838E-5 10-11 0.0 12-13 1.960675131031919E-4 14-15 0.0 16-17 5.882025393095756E-5 18-19 0.004705620314476605 20-21 0.00121561858123979 22-23 0.0 24-25 0.006313373921922779 26-27 0.006313373921922779 28-29 0.008764217835712677 30-31 0.034645129565334006 32-33 6.07809290619895E-4 34-35 0.0046664068118559675 36-37 0.01717551414783961 38-39 0.008156408545092784 40-41 0.01043079169708981 42-43 0.0062153401653711835 44-45 0.002646911426893091 46-47 0.0017057873639977695 48-49 0.0014705063482739393 50-51 0.0027253384321343677 52-53 0.002568484421651814 54-55 0.0015685401048255352 56-57 5.882025393095756E-4 58-59 8.823038089643636E-4 60-61 3.921350262063838E-5 62-63 9.803375655159595E-5 64-65 0.006117306408819587 66-67 6.07809290619895E-4 68-69 8.038768037230868E-4 70-71 0.003470394981926496 72-73 0.010156297178745341 74-75 0.005195789097234585 76-77 0.0034900017332368163 78-79 0.009489667634194489 80-81 1.5685401048255352E-4 82-83 0.004489946050063094 84-85 8.626970576540444E-4 86-87 0.0 88-89 0.0015685401048255352 90-91 7.058430471714908E-4 92-93 2.548877670341495E-4 94-95 6.274160419302141E-4 96-97 2.7449451834446866E-4 98-99 2.941012696547878E-4 100-101 7.2544979848181E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2550142.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.31228577073454 #Duplication Level Percentage of deduplicated Percentage of total 1 77.06621183413091 43.39774544071543 2 13.068376530285143 14.718203074659542 3 4.156375558172758 7.021650246069619 4 1.859496729109189 4.188500447973712 5 0.9827981039928761 2.767180384349146 6 0.6106854494608658 2.0633456127641843 7 0.40682058730289294 1.6036298018733 8 0.2718536134384883 1.2246958694203958 9 0.21613136673082947 1.0953766158629308 >10 1.221808224088628 12.564708367784739 >50 0.0937117247364012 3.590995434307636 >100 0.04391890134044562 4.378731568931198 >500 0.0013933670850945236 0.4986367212207475 >1k 2.786734170167611E-4 0.3219757333621517 >5k 1.3933670850838054E-4 0.5646246807052623 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8208 0.3218644295101998 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6184 0.24249630020602772 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4126 0.16179491181275396 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.921350262063838E-5 5 0.0 0.0 0.0 0.0 3.921350262063838E-5 6 0.0 0.0 0.0 0.0 1.1764050786191514E-4 7 0.0 0.0 0.0 0.0 1.1764050786191514E-4 8 0.0 0.0 0.0 0.0 1.5685401048255352E-4 9 0.0 0.0 0.0 3.921350262063838E-5 1.5685401048255352E-4 10-11 0.0 0.0 0.0 3.921350262063838E-5 1.5685401048255352E-4 12-13 0.0 0.0 0.0 3.921350262063838E-5 1.5685401048255352E-4 14-15 0.0 0.0 0.0 9.803375655159595E-5 1.960675131031919E-4 16-17 0.0 0.0 0.0 1.764607617928727E-4 2.3528101572383028E-4 18-19 0.0 0.0 0.0 2.3528101572383028E-4 2.7449451834446866E-4 20-21 0.0 0.0 0.0 2.7449451834446866E-4 2.7449451834446866E-4 22-23 0.0 0.0 0.0 4.7056203144766055E-4 2.7449451834446866E-4 24-25 0.0 0.0 0.0 7.2544979848181E-4 2.7449451834446866E-4 26-27 0.0 0.0 0.0 0.0010783713220675554 3.1370802096510704E-4 28-29 0.0 0.0 0.0 0.0019410683797215999 3.1370802096510704E-4 30-31 0.0 0.0 0.0 0.004038990769925753 3.1370802096510704E-4 32-33 0.0 0.0 0.0 0.007568206005783207 3.1370802096510704E-4 34-35 0.0 0.0 0.0 0.010254330935296936 3.1370802096510704E-4 36-37 0.0 0.0 0.0 0.011744444034881195 3.1370802096510704E-4 38-39 1.960675131031919E-5 0.0 0.0 0.013273770637086093 3.529215235857454E-4 40-41 3.921350262063838E-5 0.0 0.0 0.016018715820530778 3.921350262063838E-4 42-43 3.921350262063838E-5 0.0 0.0 0.019253829786733442 3.921350262063838E-4 44-45 3.921350262063838E-5 0.0 0.0 0.022332089742453556 3.921350262063838E-4 46-47 3.921350262063838E-5 0.0 0.0 0.025998552237483246 3.921350262063838E-4 48-49 3.921350262063838E-5 0.0 0.0 0.030880633313752723 3.921350262063838E-4 50-51 3.921350262063838E-5 0.0 0.0 0.038115524547260506 3.921350262063838E-4 52-53 3.921350262063838E-5 0.0 0.0 0.05550671295951363 5.489890366889373E-4 54-55 7.842700524127676E-5 0.0 0.0 0.07717217315741634 7.058430471714908E-4 56-57 7.842700524127676E-5 0.0 0.0 0.10389617519338139 7.058430471714908E-4 58-59 7.842700524127676E-5 0.0 0.0 0.1361688878501668 7.058430471714908E-4 60-61 7.842700524127676E-5 0.0 0.0 0.18036250530362624 7.450565497921293E-4 62-63 7.842700524127676E-5 0.0 0.0 0.21373319603378949 7.450565497921293E-4 64-65 7.842700524127676E-5 0.0 0.0 0.24473146985540412 7.450565497921293E-4 66-67 7.842700524127676E-5 0.0 0.0 0.30502223013463564 7.450565497921293E-4 68-69 7.842700524127676E-5 0.0 0.0 0.3869000236065286 7.450565497921293E-4 70-71 7.842700524127676E-5 0.0 0.0 0.45342573080244164 7.450565497921293E-4 72-73 7.842700524127676E-5 0.0 0.0 0.5175790210898059 7.842700524127676E-4 74-75 7.842700524127676E-5 0.0 0.0 0.6322981230064836 7.842700524127676E-4 76-77 7.842700524127676E-5 0.0 0.0 0.7203912566437476 7.842700524127676E-4 78-79 7.842700524127676E-5 0.0 0.0 0.7908579208530349 7.842700524127676E-4 80-81 7.842700524127676E-5 0.0 0.0 0.8815391456632611 8.626970576540445E-4 82-83 7.842700524127676E-5 0.0 0.0 1.0372167510671955 9.411240628953211E-4 84-85 7.842700524127676E-5 0.0 0.0 1.2009331245083605 9.411240628953211E-4 86-87 7.842700524127676E-5 0.0 0.0 1.4054315406749898 9.411240628953211E-4 88-89 7.842700524127676E-5 0.0 0.0 1.6689266715343694 9.607308142056403E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4610 0.0 45.869938 1 GTATCAA 8345 0.0 41.79639 1 ATCAACG 10355 0.0 33.25597 3 TATCAAC 10605 0.0 32.69851 2 TCAACGC 10535 0.0 32.638832 4 CAACGCA 10740 0.0 31.971617 5 AACGCAG 11035 0.0 31.158733 6 ACGCAGA 12965 0.0 26.37385 7 CGCAGAG 13045 0.0 26.066486 8 GCAGAGT 13995 0.0 23.584435 9 GTACATG 9085 0.0 22.072758 1 GAGTACT 8015 0.0 21.420576 12-13 TACATGG 9400 0.0 20.769676 2 GTACTTT 8215 0.0 20.726248 14-15 ACATGGG 9305 0.0 20.622755 3 AGTACTT 8280 0.0 20.161428 12-13 AGAGTAC 12745 0.0 20.140461 10-11 CAGAGTA 13635 0.0 18.982567 10-11 CATGGGG 5425 0.0 18.20934 4 TGGTATC 2585 0.0 17.641132 2 >>END_MODULE