FastQCFastQC Report
Thu 26 May 2016
SRR936849_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936849_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619909
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76310.2912696586026461No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51770.19760228313273476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35410.13515736615279386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44550.049.9116171
GTATCAA80750.042.1283071
ATCAACG101200.033.148273
TATCAAC102650.032.9114722
TCAACGC103050.032.5531774
CAACGCA105300.031.992975
AACGCAG110700.030.5599376
ACGCAGA126550.026.3569437
CGCAGAG127450.025.7980188
GTACATG99150.024.8701321
TACATGG101450.023.7461152
GCAGAGT139250.022.7916859
ACATGGG104500.022.4619433
GTGGTAT23700.022.2524641
GAGTACT68550.021.86584912-13
AGAGTAC122400.019.83492510-11
GTACTTT75400.019.7199614-15
CAGAGTA130000.019.71692310-11
CATGGGG61150.019.658974
TGGTATC24750.019.5822072
Produced by FastQC (version 0.11.5)