##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936849_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2619909 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.753461666034966 33.0 31.0 34.0 30.0 34.0 2 31.87914923762619 33.0 31.0 34.0 30.0 34.0 3 31.89618303536497 33.0 31.0 34.0 30.0 34.0 4 35.46572571795433 37.0 35.0 37.0 33.0 37.0 5 35.44653115814328 37.0 35.0 37.0 33.0 37.0 6 35.550139336900635 37.0 35.0 37.0 33.0 37.0 7 35.49193998722856 37.0 35.0 37.0 33.0 37.0 8 35.44919689958697 37.0 35.0 37.0 33.0 37.0 9 37.05463777558686 39.0 37.0 39.0 33.0 39.0 10-11 36.92627396600416 39.0 37.0 39.0 33.0 39.0 12-13 36.81835895826916 39.0 37.0 39.0 33.0 39.0 14-15 37.995763211623 40.0 38.0 41.0 33.0 41.0 16-17 37.91251432778772 40.0 38.0 41.0 33.0 41.0 18-19 37.82378281077702 40.0 38.0 41.0 32.5 41.0 20-21 37.702060071552104 40.0 37.0 41.0 32.0 41.0 22-23 37.5686621176537 40.0 37.0 41.0 32.0 41.0 24-25 37.70196999208751 40.0 37.0 41.0 32.0 41.0 26-27 37.66071264307271 40.0 37.0 41.0 32.0 41.0 28-29 37.56740062345676 40.0 37.0 41.0 32.0 41.0 30-31 37.404571876351426 40.0 37.0 41.0 32.0 41.0 32-33 37.23144334402454 40.0 36.5 41.0 31.0 41.0 34-35 37.05785849813867 39.5 36.0 41.0 31.0 41.0 36-37 36.8788772816155 39.0 36.0 41.0 30.0 41.0 38-39 36.72869496612287 39.0 36.0 41.0 30.0 41.0 40-41 36.546651238649886 39.0 35.5 41.0 30.0 41.0 42-43 36.341740686413154 39.0 35.0 40.5 30.0 41.0 44-45 36.10684321478341 39.0 35.0 40.0 29.5 41.0 46-47 35.80074823209509 39.0 35.0 40.0 28.5 41.0 48-49 35.58514551459612 38.5 35.0 40.0 27.0 41.0 50-51 34.90088777892667 38.0 34.0 39.5 26.5 40.5 52-53 35.10952689578149 38.0 34.5 39.5 27.5 40.5 54-55 35.513905444807435 39.0 35.0 40.0 28.0 41.0 56-57 35.41087285856112 39.0 35.0 40.0 27.5 41.0 58-59 35.15332612697617 38.0 34.5 40.0 26.5 41.0 60-61 34.86413707499001 38.0 34.0 40.0 26.0 41.0 62-63 34.514266907743746 38.0 34.0 40.0 26.0 41.0 64-65 34.169247290650176 37.0 34.0 40.0 25.5 41.0 66-67 33.78940833441162 36.5 33.0 39.5 24.5 41.0 68-69 33.37709420441702 36.0 33.0 39.0 23.0 41.0 70-71 32.97798969353516 35.5 32.5 39.0 22.5 40.0 72-73 32.56268977281272 35.0 32.0 38.0 22.0 40.0 74-75 32.102496689770526 35.0 32.0 37.0 20.5 39.5 76-77 31.65698846792007 35.0 31.0 37.0 20.0 39.0 78-79 31.22269628448927 35.0 31.0 36.0 18.0 39.0 80-81 30.768698263947336 34.5 31.0 36.0 17.0 37.5 82-83 30.34655287645487 34.0 30.0 35.0 12.0 37.0 84-85 29.94508549724437 34.0 30.0 35.0 7.5 36.5 86-87 29.58357981135986 34.0 29.5 35.0 7.0 36.0 88-89 29.226761502021635 34.0 29.0 35.0 2.0 36.0 90-91 28.836866853009017 34.0 29.0 35.0 2.0 35.5 92-93 28.445072710540707 34.0 29.0 35.0 2.0 35.0 94-95 27.99953681597338 34.0 28.5 35.0 2.0 35.0 96-97 27.453361738900092 34.0 27.0 35.0 2.0 35.0 98-99 26.746550357283404 33.0 25.5 35.0 2.0 35.0 100-101 24.061769893534468 31.0 13.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1850922854714412 1101 2 0.2636383629273986 1101 3 0.20682866628510155 1101 4 0.25294840088932347 1101 5 0.27620371390602116 1101 6 0.1001865576798906 1101 7 0.0903478789468295 1101 8 0.1356644562850704 1101 9 0.2985844334297738 1101 10-11 0.3727521680949266 1101 12-13 0.4235301403287792 1101 14-15 0.38465029214517443 1101 16-17 0.4874873035433467 1101 18-19 0.5052099093613833 1101 20-21 0.5156677566323751 1101 22-23 0.5910328934999143 1101 24-25 0.41921438101788766 1101 26-27 0.477880583332265 1101 28-29 0.3276307185214833 1101 30-31 0.27651737076669036 1101 32-33 0.500057349216604 1101 34-35 0.3535817868745639 1101 36-37 0.37914494189562475 1101 38-39 0.5227809726276575 1101 40-41 0.5067866970134176 1101 42-43 0.49083842298279023 1101 44-45 0.4102354188818751 1101 46-47 0.34993480503979413 1101 48-49 0.570828797432867 1101 50-51 0.5198854360995782 1101 52-53 0.47371875153955756 1101 54-55 0.5039146307898577 1101 56-57 0.22817014893519882 1101 58-59 0.3123193386151115 1101 60-61 0.49663469792382386 1101 62-63 0.48082300817196 1101 64-65 0.4166255704075894 1101 66-67 0.5059479045413369 1101 68-69 0.45305343567408585 1101 70-71 0.2785776994352531 1101 72-73 0.14522670200619814 1101 74-75 0.22435207583220063 1101 76-77 0.4624764446974119 1101 78-79 0.45820284728231897 1101 80-81 0.2811870296159178 1101 82-83 0.3336483311487335 1101 84-85 0.328979989473968 1101 86-87 0.2552466675047924 1101 88-89 0.3765203350554813 1101 90-91 0.29186434191004906 1101 92-93 0.39901769095746786 1101 94-95 0.5525134232199207 1101 96-97 0.4830431199138552 1101 98-99 0.4113562975670746 1101 100-101 0.8587679604767793 1102 1 0.20113638538209955 1102 2 0.2653382952611416 1102 3 0.22469346544970747 1102 4 0.14978279952584472 1102 5 0.16627504240467061 1102 6 0.08433605265960864 1102 7 0.12572363633013595 1102 8 0.24364749300789867 1102 9 0.29339231410273925 1102 10-11 0.32116121411349496 1102 12-13 0.22554754866576587 1102 14-15 0.24923857441903152 1102 16-17 0.4025600818580699 1102 18-19 0.4350699533744873 1102 20-21 0.3925140039137389 1102 22-23 0.45755902507062274 1102 24-25 0.10453397871814474 1102 26-27 0.20745555611066635 1102 28-29 0.283413780427594 1102 30-31 0.22227916911241863 1102 32-33 0.48294455401658354 1102 34-35 0.13500898704248243 1102 36-37 0.16064607348780413 1102 38-39 0.15143544400142162 1102 40-41 0.24604527639964147 1102 42-43 0.31656409424149956 1102 44-45 0.0917056782806327 1102 46-47 0.0776924502295202 1102 48-49 0.2443906626479162 1102 50-51 0.26542567312397836 1102 52-53 0.3854878496019225 1102 54-55 0.29328198009817896 1102 56-57 0.3349691794159142 1102 58-59 0.3103105617412609 1102 60-61 0.1969116771618431 1102 62-63 0.4822747246377119 1102 64-65 0.546442101547612 1102 66-67 0.41437169761083226 1102 68-69 0.4994191667056782 1102 70-71 0.44657000272034253 1102 72-73 0.20105375494881628 1102 74-75 0.23743193023021547 1102 76-77 0.39136305829120843 1102 78-79 0.34187401943378504 1102 80-81 0.4852533315133556 1102 82-83 0.5074882054922831 1102 84-85 0.5484981429489864 1102 86-87 0.5935121749888488 1102 88-89 0.6233630770310086 1102 90-91 0.4651519987829076 1102 92-93 0.43880861338010746 1102 94-95 0.4719469295053784 1102 96-97 0.27823597119877164 1102 98-99 0.2508412444302408 1102 100-101 0.3394396133664124 1103 1 0.19089804532163157 1103 2 0.2686838346340572 1103 3 0.17841614382107807 1103 4 0.25356032833997943 1103 5 0.25489776528255703 1103 6 0.28089266034235294 1103 7 0.1826191687968688 1103 8 0.2307091728986279 1103 9 0.22565560850566158 1103 10-11 0.23938635569025024 1103 12-13 0.4429362747051542 1103 14-15 0.4285936903661707 1103 16-17 0.4274616303778416 1103 18-19 0.45395980742381425 1103 20-21 0.42650648384744017 1103 22-23 0.41971700785120447 1103 24-25 0.12264002700003829 1103 26-27 0.3357523685233801 1103 28-29 0.295773884173812 1103 30-31 0.38592661612664614 1103 32-33 0.5298953666441548 1103 34-35 0.27881530696758716 1103 36-37 0.4064339462298605 1103 38-39 0.47525668128253074 1103 40-41 0.3347963622051324 1103 42-43 0.2756030604625863 1103 44-45 0.3309040085189707 1103 46-47 0.3293824393331022 1103 48-49 0.32455131581939156 1103 50-51 0.28702692544521824 1103 52-53 0.38767842956785614 1103 54-55 0.4619646179532779 1103 56-57 0.4954385543295814 1103 58-59 0.5478598795638163 1103 60-61 0.5349676939822388 1103 62-63 0.6170683832267017 1103 64-65 0.6126639895446644 1103 66-67 0.45532604006543664 1103 68-69 0.43674656583703353 1103 70-71 0.4294864992902703 1103 72-73 0.2176171153441544 1103 74-75 0.28141234736368403 1103 76-77 0.2653508727143823 1103 78-79 0.33836739717132147 1103 80-81 0.37753848014976654 1103 82-83 0.41607520038170875 1103 84-85 0.39622066784961163 1103 86-87 0.43911226093246114 1103 88-89 0.3783140738234678 1103 90-91 0.403952778414272 1103 92-93 0.5957202295804791 1103 94-95 0.7220107426394051 1103 96-97 0.4092746918666741 1103 98-99 0.41803337445065125 1103 100-101 0.6498376857739068 1104 1 0.43334807914488493 1104 2 0.4257487137927818 1104 3 0.37161065830358453 1104 4 0.3470717974993178 1104 5 0.35788823327345654 1104 6 0.25904726338029604 1104 7 0.3258745651439554 1104 8 0.3208599631329321 1104 9 0.3417077578199752 1104 10-11 0.44672120594546527 1104 12-13 0.4971178748527407 1104 14-15 0.6286567246199084 1104 16-17 0.6083090567353864 1104 18-19 0.48213298250529846 1104 20-21 0.7693566160656289 1104 22-23 0.6935619128386037 1104 24-25 0.6336579225784149 1104 26-27 0.4849041427070091 1104 28-29 0.5666681711180317 1104 30-31 0.46181887367538366 1104 32-33 0.581563652727425 1104 34-35 0.5214446655556202 1104 36-37 0.5298477584185335 1104 38-39 0.5318201152656812 1104 40-41 0.6137012266046185 1104 42-43 0.5284193079258444 1104 44-45 0.4562071264084011 1104 46-47 0.5745643469648556 1104 48-49 0.7471958641251462 1104 50-51 0.4642030523746783 1104 52-53 0.48917470900946824 1104 54-55 0.4700195961218938 1104 56-57 0.6164507307024962 1104 58-59 0.6379586434774751 1104 60-61 0.5330186748567272 1104 62-63 0.6647850058206402 1104 64-65 0.6987713630149273 1104 66-67 0.652258824027065 1104 68-69 0.5629372060190647 1104 70-71 0.576668468418859 1104 72-73 0.5230329569283185 1104 74-75 0.4992338220577395 1104 76-77 0.49938088316885043 1104 78-79 0.5710146513377303 1104 80-81 0.6360784223427451 1104 82-83 0.7058495070049204 1104 84-85 0.6800818694977266 1104 86-87 0.6112219712823794 1104 88-89 0.609194216496121 1104 90-91 0.46256510237958537 1104 92-93 0.20577244039162323 1104 94-95 0.47118779202136096 1104 96-97 0.3978419386534675 1104 98-99 0.5110757457685295 1104 100-101 0.6458611775640826 1105 1 0.14175921945222214 1105 2 0.2622949232998728 1105 3 0.22053790781399485 1105 4 0.28078101392284793 1105 5 0.36862483741482777 1105 6 0.3282899938414374 1105 7 0.17046551867411353 1105 8 0.2674137755651316 1105 9 0.28104511032373125 1105 10-11 0.2827715065744272 1105 12-13 0.41532816953685625 1105 14-15 0.3920054441033116 1105 16-17 0.5591028647999465 1105 18-19 0.5981005912134734 1105 20-21 0.5070516558647213 1105 22-23 0.44162377355341675 1105 24-25 0.3186289010404977 1105 26-27 0.3185607098852188 1105 28-29 0.3376682186363169 1105 30-31 0.13075950918690893 1105 32-33 0.37707024310871873 1105 34-35 0.13389415210541955 1105 36-37 0.3665847993236824 1105 38-39 0.4266659487717277 1105 40-41 0.3549148940757263 1105 42-43 0.31026881550501173 1105 44-45 0.2835107773289991 1105 46-47 0.28179397552354146 1105 48-49 0.1711353976169434 1105 50-51 0.43232953289774656 1105 52-53 0.32011439314432266 1105 54-55 0.3714441841030123 1105 56-57 0.5394755372677054 1105 58-59 0.502276761187332 1105 60-61 0.2948652605670645 1105 62-63 0.3546741264594502 1105 64-65 0.3582755754600697 1105 66-67 0.461803275357731 1105 68-69 0.47758325042540406 1105 70-71 0.5162373461684737 1105 72-73 0.43833048203465097 1105 74-75 0.3895513907797863 1105 76-77 0.42310746904095353 1105 78-79 0.569382221424668 1105 80-81 0.411534067059673 1105 82-83 0.41572992269889397 1105 84-85 0.45801617395645877 1105 86-87 0.2634847008740344 1105 88-89 0.2598938979214296 1105 90-91 0.21900825282145675 1105 92-93 0.17516169398051673 1105 94-95 0.19995697752767683 1105 96-97 0.054761965385029754 1105 98-99 0.010348817158174484 1105 100-101 0.17232784955549363 1106 1 0.06396127918656092 1106 2 0.25519751220348397 1106 3 0.29542336098933575 1106 4 0.2886660367536322 1106 5 0.3685277938007303 1106 6 0.3888918318815371 1106 7 0.3550962984987862 1106 8 0.37696475306017874 1106 9 0.3084272560274499 1106 10-11 0.3286878039034846 1106 12-13 0.42269608479643495 1106 14-15 0.5223005745569083 1106 16-17 0.44698972635184475 1106 18-19 0.31717340692673446 1106 20-21 0.5199917866339376 1106 22-23 0.4506906723564441 1106 24-25 0.47658977766519683 1106 26-27 0.4102007888716557 1106 28-29 0.5549795814351128 1106 30-31 0.4312202895537638 1106 32-33 0.3831321949137845 1106 34-35 0.2733285384427262 1106 36-37 0.23911041634951147 1106 38-39 0.2672065275393223 1106 40-41 0.3648086935257311 1106 42-43 0.2436553809494697 1106 44-45 0.47750633139564513 1106 46-47 0.6236424167097425 1106 48-49 0.44640923478954164 1106 50-51 0.333532127223215 1106 52-53 0.43360434605263265 1106 54-55 0.23623938016908852 1106 56-57 0.4859110637692581 1106 58-59 0.4020828311147895 1106 60-61 0.4337091345481383 1106 62-63 0.5337561331589953 1106 64-65 0.5060876675920412 1106 66-67 0.5138556495736211 1106 68-69 0.42663921643368496 1106 70-71 0.41337249033652057 1106 72-73 0.29723895484580964 1106 74-75 0.5077198019178297 1106 76-77 0.41828416568680993 1106 78-79 0.49658340239699683 1106 80-81 0.4253390122765204 1106 82-83 0.4946585323873798 1106 84-85 0.44445658799873655 1106 86-87 0.5123955467355081 1106 88-89 0.5455886607879563 1106 90-91 0.6383409123062833 1106 92-93 0.5101362125014397 1106 94-95 0.4908350611507224 1106 96-97 0.5691887231251407 1106 98-99 0.7101369320525777 1106 100-101 0.608018513278811 1107 1 0.12529547536430385 1107 2 0.19117152812885152 1107 3 0.1924363948328427 1107 4 0.016584437437188626 1107 5 0.25120886869499515 1107 6 0.1594536102187334 1107 7 0.18642131987634514 1107 8 0.2618796057915631 1107 9 0.22007086447781887 1107 10-11 0.2587217571673506 1107 12-13 0.20816714568761796 1107 14-15 0.2173013104786108 1107 16-17 0.33829229979503594 1107 18-19 0.2160395831824431 1107 20-21 0.28079254907270723 1107 22-23 0.3569769168318828 1107 24-25 0.26484552910230263 1107 26-27 0.12411411521251381 1107 28-29 0.20942797925593482 1107 30-31 0.20272947583405454 1107 32-33 0.31738326485368873 1107 34-35 0.32283854996398276 1107 36-37 0.43100973005109466 1107 38-39 0.34407617012557523 1107 40-41 0.39715965212844395 1107 42-43 0.39154498446617936 1107 44-45 0.477133194541679 1107 46-47 0.3747998841782234 1107 48-49 0.5182540385253986 1107 50-51 0.3777947316905923 1107 52-53 0.6258213204548966 1107 54-55 0.5629021484636425 1107 56-57 0.713574579399264 1107 58-59 0.5693030869713382 1107 60-61 0.6524178301962777 1107 62-63 0.629975804923312 1107 64-65 0.7245786392462463 1107 66-67 0.7063764156382462 1107 68-69 0.5682195356179918 1107 70-71 0.5056521607550835 1107 72-73 0.5271035333086616 1107 74-75 0.5305542776661909 1107 76-77 0.5364668199924658 1107 78-79 0.5905664428856952 1107 80-81 0.6246522228075939 1107 82-83 0.5783346211964755 1107 84-85 0.5497695710510371 1107 86-87 0.6095931515990465 1107 88-89 0.46965016818121796 1107 90-91 0.43384207498294103 1107 92-93 0.4311017391154621 1107 94-95 0.45553457615908144 1107 96-97 0.34405912724767873 1107 98-99 0.3524282219381689 1107 100-101 0.3359867495976694 1108 1 0.04131011893661807 1108 2 0.20249393067417643 1108 3 0.10366469691123825 1108 4 0.272955070577126 1108 5 0.3297889986647746 1108 6 0.194552309299155 1108 7 0.05753639136077737 1108 8 0.17328998695297315 1108 9 0.27448347748738655 1108 10-11 0.19383861160799398 1108 12-13 0.2059659067879096 1108 14-15 0.24238940404360676 1108 16-17 0.31646763879909656 1108 18-19 0.3284803795642901 1108 20-21 0.40002426467410857 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-2.000617086190662 2316 96-97 -1.9702683063503024 2316 98-99 -2.0577453938938817 2316 100-101 -2.031153879169171 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4980.0 3 1800.0 4 1917.0 5 2112.0 6 2494.0 7 2880.0 8 3479.0 9 4466.0 10 5279.0 11 6476.0 12 8193.0 13 9673.0 14 11043.0 15 13107.0 16 16036.0 17 20209.0 18 19178.0 19 13748.0 20 14090.0 21 14825.0 22 16693.0 23 18669.0 24 21571.0 25 24703.0 26 28922.0 27 35056.0 28 41467.0 29 50552.0 30 62215.0 31 77608.0 32 98595.0 33 129643.0 34 175894.0 35 249472.0 36 372704.0 37 532155.0 38 443388.0 39 64581.0 40 36.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.344851664736765 18.83340720794807 14.555017117149106 23.26672401016606 2 15.167188045754115 20.034317496006153 40.70345140867786 24.09504304956187 3 19.23885317980506 24.478074351071488 29.237188820083475 27.045883649039975 4 12.945093407101032 16.663052599188973 37.322983865733825 33.06887012797616 5 14.384134751802844 36.83600897153832 33.817795893302396 14.962060383356446 6 31.903117437135386 35.90356495634396 17.885341913075887 14.30797569344476 7 28.71335621772339 32.128454418020844 20.858019563710325 18.300169800545437 8 26.17396766722483 35.03716531644458 19.88356840499407 18.90529861133652 9 26.225933096595195 17.244251986000354 20.222479382134072 36.30733553527038 10-11 24.721088718459807 26.746009915106363 27.59614887205214 20.93675249438169 12-13 25.80128899629994 23.89576308187679 27.966950927534 22.335996994289264 14-15 22.781708939781165 25.309506234981367 26.03230939876986 25.87647542646761 16-17 22.469718894451663 28.67512897993947 26.377034670594142 22.478117455014733 18-19 22.337831051452874 27.342519899210295 28.58400996979019 21.73563907954664 20-21 22.99951595035208 26.520996226092414 28.617507632943777 21.861980190611728 22-23 22.756331955073197 26.496892043754162 28.44411709917976 22.302658901992878 24-25 22.633729619429083 26.652808454171232 28.491203654842717 22.222258271556967 26-27 22.53468012337072 26.89156892311316 28.291694156494394 22.282056797021728 28-29 22.300290684414747 26.837055459059417 28.595227630268354 22.26742622625748 30-31 22.56118437701462 26.564090584825657 28.51841418919512 22.3563108489646 32-33 22.270370831230835 26.872897341804908 28.404140161181846 22.45259166578241 34-35 22.709595146089097 26.780291521687023 28.235285361165353 22.274827971058524 36-37 22.68187559186216 26.58120950002462 28.197162573203876 22.53975233490934 38-39 22.51643331546374 26.766317805616346 28.394772866894296 22.322476012025618 40-41 22.70745766645241 26.694353389508336 28.28698564987778 22.311203294161473 42-43 22.53491155789559 26.697713582090348 28.170190075521795 22.59718478449227 44-45 22.66982530476767 26.849384452988858 27.99408222930178 22.48670801294169 46-47 22.749683290526505 26.914980634823575 27.722565936450465 22.612770138199455 48-49 22.760347395137636 27.07498019484343 27.51906228428315 22.64561012573579 50-51 22.664418238225522 27.16389608168849 27.46827746360172 22.70340821648427 52-53 22.95090226829528 27.17475152649131 27.321436682434463 22.55290952277895 54-55 22.788100243830566 27.023663932161053 27.389791484404462 22.798444339603915 56-57 22.710392295881803 27.385995062783948 27.297480149509667 22.606132491824585 58-59 22.856243141371248 27.060642206212126 27.364988787633 22.718125864783627 60-61 22.779595751933236 26.94464176451346 27.704558354425696 22.57120412912761 62-63 22.796643616948185 26.98920241052521 27.711020198149207 22.503133774377407 64-65 22.697826458697218 27.040882149967633 27.504091802958314 22.757199588376835 66-67 22.62746722298676 27.30603394799578 27.319566574222353 22.74693225479511 68-69 22.506452540187844 27.172571521936728 27.551840782497187 22.769135155378237 70-71 22.561407460717742 27.101312049358867 27.490039840637447 22.84724064928594 72-73 22.658803170365637 27.12629447720901 27.359817182682367 22.855085169742985 74-75 22.556292091056886 27.31643617773643 27.270910406618626 22.856361324588057 76-77 22.655038937277308 27.219993707723766 27.288834569643434 22.83613278535549 78-79 22.63630043609573 27.223437310904608 27.18646289826711 22.953799354732553 80-81 22.540497740191633 27.368970542775973 27.24007048547645 22.85046123155594 82-83 22.619152394139952 27.361422167374922 27.25059693847453 22.768828500010596 84-85 22.676890661903222 27.254305242797617 27.130536133650786 22.93826796164837 86-87 22.698838470185084 27.071224321865134 27.344308755861523 22.885628452088255 88-89 22.702319825971536 27.07988692043718 27.19303519678696 23.02475805680433 90-91 22.618479262013047 27.113613912180316 27.37782698539202 22.890079840414618 92-93 22.70708336483311 27.106896122510847 27.303435652251608 22.882584860404435 94-95 22.635882039130056 27.097101749409973 27.182477460754946 23.084538750705022 96-97 22.60951943196607 27.092952984648132 27.178296272344156 23.119231311041638 98-99 22.46184143109237 27.131317095817735 27.110738308843686 23.296103164246208 100-101 22.55653438303894 27.052441623235467 27.106739374231786 23.284284619493807 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 315.0 1 226.5 2 184.0 3 244.0 4 227.5 5 235.0 6 392.5 7 804.5 8 1436.5 9 1807.5 10 1703.0 11 1592.0 12 1657.5 13 1852.0 14 2091.0 15 2226.5 16 2347.0 17 2517.0 18 2659.5 19 2907.0 20 3273.5 21 3793.5 22 4533.5 23 5562.5 24 6951.5 25 8838.0 26 11731.0 27 15241.0 28 18379.5 29 22031.0 30 26868.0 31 32085.5 32 37213.0 33 42559.5 34 49499.0 35 55493.5 36 61189.5 37 67281.5 38 71386.0 39 73931.0 40 76689.5 41 81735.0 42 86541.5 43 91404.0 44 97032.5 45 101169.0 46 102165.5 47 103071.0 48 106923.0 49 107552.0 50 104358.5 51 100939.0 52 96559.5 53 92937.5 54 89534.0 55 83489.0 56 76258.0 57 68459.0 58 61282.5 59 54373.0 60 45509.0 61 36501.5 62 28549.5 63 21813.5 64 16542.5 65 12392.5 66 9112.5 67 6927.0 68 5056.0 69 3380.0 70 2259.0 71 1473.5 72 992.0 73 625.5 74 378.5 75 266.0 76 191.5 77 103.5 78 52.0 79 38.5 80 32.5 81 30.5 82 19.0 83 14.5 84 6.5 85 7.0 86 12.0 87 9.0 88 5.0 89 4.5 90 3.5 91 2.5 92 4.0 93 5.0 94 2.0 95 0.5 96 1.0 97 1.5 98 1.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.023130574382545347 2 0.00969499322304706 3 0.010801901898119363 4 0.01965717129869778 5 0.05366598610867782 6 0.03919983480342256 7 0.01393178160004794 8 0.045955794647829375 9 0.026718485260365913 10-11 0.021851904016513553 12-13 0.03414240723628187 14-15 0.01734793078690901 16-17 0.015744821671287056 18-19 0.01509594417210674 20-21 0.013092057777579297 22-23 0.006908636903037472 24-25 0.0016221937479507875 26-27 1.9084632328832794E-5 28-29 0.0021947327178157713 30-31 0.0 32-33 4.198619112343215E-4 34-35 3.816926465766559E-5 36-37 0.0 38-39 9.542316164416398E-5 40-41 3.816926465766559E-5 42-43 1.1450779397299677E-4 44-45 1.3359242630182956E-4 46-47 0.0 48-49 4.007772789054887E-4 50-51 1.9084632328832796E-4 52-53 4.580311758919871E-4 54-55 0.002175648085486939 56-57 4.389465435631543E-4 58-59 0.0012977549983606302 60-61 0.0031298797019285785 62-63 0.0024810022027482634 64-65 0.001412262792333627 66-67 0.011985149102506996 68-69 0.015191367333750906 70-71 0.02763454761214989 72-73 0.017691454168828 74-75 0.01959991740171128 76-77 0.03148964334257411 78-79 0.020229710268562764 80-81 0.04631840266207719 82-83 0.03513480811738118 84-85 0.01967625593102661 86-87 0.014866928584160747 88-89 0.012882126821962136 90-91 0.023722197984739166 92-93 0.03217669010641209 94-95 0.01188972594086283 96-97 0.01862660115294081 98-99 0.02698567011296957 100-101 0.03877997289218824 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2619909.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.78755405673239 #Duplication Level Percentage of deduplicated Percentage of total 1 77.97128394157494 45.05769785646592 2 12.618548498248932 14.583901069201188 3 3.9678671838343886 6.878800181272927 4 1.7829424052784124 4.12127522500267 5 0.9530982041226435 2.7538606996055908 6 0.57714733644878 2.0011159742239792 7 0.3959019573649341 1.6014744033674602 8 0.26999141541833294 1.2481714810672486 9 0.21051831251074557 1.0948804527732114 >10 1.1249365928622297 11.952773027843982 >50 0.08558366567103984 3.3947643152758245 >100 0.04059022434210804 4.125120011523927 >500 0.0012589576720642248 0.4663408618319099 >1k 1.9878279032051725E-4 0.22939880968666396 >5k 1.3252186021367815E-4 0.4904256308574181 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7631 0.2912696586026461 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5177 0.19760228313273476 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3541 0.13515736615279386 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.816926465766559E-5 3.816926465766559E-5 5 0.0 0.0 0.0 3.816926465766559E-5 3.816926465766559E-5 6 0.0 0.0 0.0 3.816926465766559E-5 3.816926465766559E-5 7 0.0 0.0 0.0 3.816926465766559E-5 3.816926465766559E-5 8 0.0 0.0 0.0 3.816926465766559E-5 3.816926465766559E-5 9 0.0 0.0 0.0 7.633852931533118E-5 3.816926465766559E-5 10-11 0.0 0.0 0.0 7.633852931533118E-5 3.816926465766559E-5 12-13 0.0 3.816926465766559E-5 0.0 7.633852931533118E-5 3.816926465766559E-5 14-15 0.0 3.816926465766559E-5 0.0 7.633852931533118E-5 7.633852931533118E-5 16-17 0.0 3.816926465766559E-5 0.0 7.633852931533118E-5 7.633852931533118E-5 18-19 0.0 3.816926465766559E-5 0.0 1.1450779397299677E-4 7.633852931533118E-5 20-21 0.0 3.816926465766559E-5 0.0 1.3359242630182956E-4 7.633852931533118E-5 22-23 0.0 3.816926465766559E-5 0.0 1.9084632328832796E-4 9.542316164416398E-5 24-25 0.0 3.816926465766559E-5 0.0 4.007772789054887E-4 1.5267705863066235E-4 26-27 0.0 3.816926465766559E-5 0.0 8.39723822468643E-4 1.5267705863066235E-4 28-29 0.0 3.816926465766559E-5 0.0 0.0018512093358967812 1.5267705863066235E-4 30-31 0.0 3.816926465766559E-5 0.0 0.0038741803627530573 1.5267705863066235E-4 32-33 0.0 3.816926465766559E-5 0.0 0.007691106828519617 1.5267705863066235E-4 34-35 0.0 3.816926465766559E-5 0.0 0.01017210903126788 1.5267705863066235E-4 36-37 0.0 3.816926465766559E-5 0.0 0.011851556676205166 1.5267705863066235E-4 38-39 0.0 3.816926465766559E-5 0.0 0.013607342850457782 1.5267705863066235E-4 40-41 0.0 3.816926465766559E-5 0.0 0.015477636818683397 1.5267705863066235E-4 42-43 0.0 3.816926465766559E-5 0.0 0.018187654609377654 1.5267705863066235E-4 44-45 0.0 3.816926465766559E-5 0.0 0.021451126737608062 1.5267705863066235E-4 46-47 0.0 3.816926465766559E-5 0.0 0.025993269231870265 1.7176169095949517E-4 48-49 0.0 3.816926465766559E-5 0.0 0.030917104372709128 1.9084632328832796E-4 50-51 0.0 3.816926465766559E-5 0.0 0.037673064217115936 2.0993095561716075E-4 52-53 0.0 3.816926465766559E-5 0.0 0.054658386989777125 2.4810022027482633E-4 54-55 0.0 3.816926465766559E-5 0.0 0.07710191460848449 2.671848526036591E-4 56-57 0.0 3.816926465766559E-5 0.0 0.10420209251542706 2.671848526036591E-4 58-59 0.0 3.816926465766559E-5 0.0 0.13439398085964055 2.671848526036591E-4 60-61 0.0 3.816926465766559E-5 0.0 0.177601588452118 2.671848526036591E-4 62-63 0.0 3.816926465766559E-5 0.0 0.20939658591195343 3.053541172613247E-4 64-65 0.0 3.816926465766559E-5 0.0 0.2393403740358921 3.053541172613247E-4 66-67 0.0 3.816926465766559E-5 0.0 0.2980828723440394 3.2443874959015755E-4 68-69 0.0 3.816926465766559E-5 0.0 0.38001319893171864 3.4352338191899034E-4 70-71 0.0 3.816926465766559E-5 0.0 0.44440474840920047 3.4352338191899034E-4 72-73 0.0 3.816926465766559E-5 0.0 0.5073649504620199 4.580311758919871E-4 74-75 0.0 3.816926465766559E-5 0.0 0.6139907912832088 4.580311758919871E-4 76-77 0.0 3.816926465766559E-5 0.0 0.6992609285284337 4.580311758919871E-4 78-79 0.0 3.816926465766559E-5 0.0 0.7719542930689578 4.580311758919871E-4 80-81 0.0 3.816926465766559E-5 0.0 0.8638277130999588 5.343697052073182E-4 82-83 0.0 3.816926465766559E-5 0.0 1.0123443218829355 6.48877499180315E-4 84-85 0.0 3.816926465766559E-5 0.0 1.174849966162947 6.48877499180315E-4 86-87 0.0 3.816926465766559E-5 0.0 1.3775669307598086 7.252160284956463E-4 88-89 0.0 3.816926465766559E-5 0.0 1.6275756142675184 7.824699254821446E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4455 0.0 49.911617 1 GTATCAA 8075 0.0 42.128307 1 ATCAACG 10120 0.0 33.14827 3 TATCAAC 10265 0.0 32.911472 2 TCAACGC 10305 0.0 32.553177 4 CAACGCA 10530 0.0 31.99297 5 AACGCAG 11070 0.0 30.559937 6 ACGCAGA 12655 0.0 26.356943 7 CGCAGAG 12745 0.0 25.798018 8 GTACATG 9915 0.0 24.870132 1 TACATGG 10145 0.0 23.746115 2 GCAGAGT 13925 0.0 22.791685 9 ACATGGG 10450 0.0 22.461943 3 GTGGTAT 2370 0.0 22.252464 1 GAGTACT 6855 0.0 21.865849 12-13 AGAGTAC 12240 0.0 19.834925 10-11 GTACTTT 7540 0.0 19.71996 14-15 CAGAGTA 13000 0.0 19.716923 10-11 CATGGGG 6115 0.0 19.65897 4 TGGTATC 2475 0.0 19.582207 2 >>END_MODULE