FastQCFastQC Report
Thu 26 May 2016
SRR936849_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936849_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2619909
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80920.30886568960982996No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61790.23584788631971568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40560.15481453745149165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49750.047.269161
GTATCAA86550.038.6430471
ATCAACG108650.030.5560473
TCAACGC109850.030.265494
TATCAAC111950.029.8256032
CAACGCA112700.029.5479055
AACGCAG115600.028.8477466
ACGCAGA134600.024.5991657
CGCAGAG136250.024.196678
GTACATG92150.023.0450671
TACATGG94250.022.1228542
GCAGAGT149200.021.7462569
ACATGGG94700.021.5659313
GAGTACT81450.021.37138212-13
GTGGTAT27550.020.8655361
GTACTTT86250.019.85218414-15
AGAGTAC130850.019.76772910-11
CAGAGTA141050.019.04548810-11
TGGTATC28000.018.8288142
AGTACTT88100.018.30263512-13
Produced by FastQC (version 0.11.5)