FastQCFastQC Report
Thu 26 May 2016
SRR936848_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936848_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647031
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78390.29614311279316335No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54610.2063066129561762No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33930.12818134732838415No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44650.048.6467131
GTATCAA82750.041.641731
ATCAACG104300.032.44983
TCAACGC104850.032.2801864
TATCAAC105750.032.094152
CAACGCA106850.031.8088385
AACGCAG110100.030.8657996
ACGCAGA125750.026.6797397
CGCAGAG127350.026.1206478
GTACATG97650.024.4337561
GCAGAGT136850.023.43669
TACATGG101100.023.2735232
GAGTACT69500.022.25451912-13
ACATGGG103550.021.8510913
GTACTTT74700.020.48425314-15
AGAGTAC122250.020.21053310-11
CATGGGG59500.019.9731944
CGCGGAA2400.019.79477182-83
CAGAGTA130950.019.68418910-11
AGTACTT75350.019.45467412-13
Produced by FastQC (version 0.11.5)