##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936848_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2647031 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.696883413907884 33.0 31.0 34.0 30.0 34.0 2 31.790789000959943 33.0 31.0 34.0 30.0 34.0 3 31.818040287401242 33.0 31.0 34.0 30.0 34.0 4 35.417810747210744 37.0 35.0 37.0 33.0 37.0 5 35.4140476632121 37.0 35.0 37.0 33.0 37.0 6 35.47530119594368 37.0 35.0 37.0 33.0 37.0 7 35.41487236076948 37.0 35.0 37.0 33.0 37.0 8 35.37739565573656 37.0 35.0 37.0 33.0 37.0 9 36.99780131022266 39.0 37.0 39.0 33.0 39.0 10-11 36.85959949090132 39.0 37.0 39.0 33.0 39.0 12-13 36.762739839465425 39.0 37.0 39.0 32.5 39.0 14-15 37.91076398425255 40.0 38.0 41.0 33.0 41.0 16-17 37.838293922511674 40.0 38.0 41.0 33.0 41.0 18-19 37.77072765675959 40.0 37.5 41.0 32.0 41.0 20-21 37.690747671636636 40.0 37.0 41.0 32.0 41.0 22-23 37.574537094578794 40.0 37.0 41.0 32.0 41.0 24-25 37.53769392953842 40.0 37.0 41.0 32.0 41.0 26-27 37.57677091805876 40.0 37.0 41.0 32.0 41.0 28-29 37.50099621047128 40.0 37.0 41.0 32.0 41.0 30-31 37.36439901912747 40.0 37.0 41.0 31.5 41.0 32-33 37.20270673067297 40.0 36.5 41.0 31.0 41.0 34-35 37.04832735241862 39.0 36.0 41.0 31.0 41.0 36-37 36.886626186093025 39.0 36.0 41.0 30.0 41.0 38-39 36.74237286983039 39.0 36.0 41.0 30.0 41.0 40-41 36.56305234052793 39.0 35.5 41.0 30.0 41.0 42-43 36.360856937451814 39.0 35.0 40.5 30.0 41.0 44-45 36.144808844324075 39.0 35.0 40.0 29.5 41.0 46-47 35.848443029190065 39.0 35.0 40.0 28.5 41.0 48-49 35.645282393746044 38.5 35.0 40.0 27.5 41.0 50-51 34.975243962008754 38.0 34.0 39.5 26.5 40.5 52-53 35.17486081575924 38.0 34.5 40.0 27.5 40.5 54-55 35.56312544129631 39.0 35.0 40.0 28.0 41.0 56-57 35.44783570725088 39.0 35.0 40.0 27.5 41.0 58-59 35.223473960070734 38.0 34.5 40.0 27.0 41.0 60-61 34.93633187522171 38.0 34.0 40.0 26.0 41.0 62-63 34.61792192837938 38.0 34.0 40.0 26.0 41.0 64-65 34.27997499840387 37.0 34.0 40.0 26.0 41.0 66-67 33.90627725931431 36.5 33.5 39.5 25.0 41.0 68-69 33.504588159337764 36.0 33.0 39.0 24.0 41.0 70-71 33.09232022594371 35.5 33.0 39.0 23.0 40.5 72-73 32.70170390902109 35.0 32.0 38.0 22.5 40.0 74-75 32.297037322192296 35.0 32.0 37.0 22.0 39.5 76-77 31.836646416305665 35.0 31.5 37.0 20.5 39.0 78-79 31.37877172575614 35.0 31.0 36.0 19.5 39.0 80-81 30.93452513400863 35.0 31.0 36.0 18.0 37.5 82-83 30.539994809278774 34.0 31.0 35.0 16.0 37.0 84-85 30.172215399064083 34.0 30.0 35.0 11.5 37.0 86-87 29.81172509879937 34.0 30.0 35.0 7.0 36.0 88-89 29.457985758383636 34.0 29.5 35.0 4.5 36.0 90-91 29.09212491277964 34.0 29.0 35.0 2.0 35.5 92-93 28.702763020153522 34.0 29.0 35.0 2.0 35.0 94-95 28.31745982574439 34.0 29.0 35.0 2.0 35.0 96-97 27.794950455812568 34.0 28.0 35.0 2.0 35.0 98-99 27.18709338878162 34.0 27.0 35.0 2.0 35.0 100-101 24.896910916419188 31.0 21.5 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2675533531950549 1101 2 0.28719178929782885 1101 3 0.29179391115608055 1101 4 0.2987858211758265 1101 5 0.19589242305969634 1101 6 0.20784090879415373 1101 7 0.20986824151450634 1101 8 0.29782374338294204 1101 9 0.24043548232120315 1101 10-11 0.20700666602589024 1101 12-13 0.3604038938306431 1101 14-15 0.3596174541904986 1101 16-17 0.40746753914039857 1101 18-19 0.5809352221542525 1101 20-21 0.4492897816430954 1101 22-23 0.5975879565253024 1101 24-25 0.5440585613500559 1101 26-27 0.4566928281088707 1101 28-29 0.3901643547821081 1101 30-31 0.3742238109578011 1101 32-33 0.2876676937726188 1101 34-35 0.4364034696660539 1101 36-37 0.49316222515619046 1101 38-39 0.43741660321057907 1101 40-41 0.5113032551047283 1101 42-43 0.5154052427252864 1101 44-45 0.4458823416534301 1101 46-47 0.3192551222655169 1101 48-49 0.3486024262629357 1101 50-51 0.33971739828105996 1101 52-53 0.2152248198084976 1101 54-55 0.3063604457895721 1101 56-57 0.36342482740707993 1101 58-59 0.456162905206277 1101 60-61 0.5056744676728826 1101 62-63 0.4600030728683535 1101 64-65 0.2300592318318806 1101 66-67 0.3776158894246535 1101 68-69 0.4371168587486096 1101 70-71 0.43592708170566397 1101 72-73 0.330804544603879 1101 74-75 0.343773392746769 1101 76-77 0.2986481154927034 1101 78-79 0.3755338252591862 1101 80-81 0.3733554192858328 1101 82-83 0.30723171130216187 1101 84-85 0.2890983118057129 1101 86-87 0.3504116115601619 1101 88-89 0.3505751891285058 1101 90-91 0.48717496780255587 1101 92-93 0.376787356941243 1101 94-95 0.49776943857559175 1101 96-97 0.5329678133962652 1101 98-99 0.49132717714667606 1101 100-101 0.727823426022983 1102 1 0.07564512423692804 1102 2 0.06963374377989595 1102 3 0.15061126488351917 1102 4 0.21436676874661487 1102 5 0.1702660072819171 1102 6 0.05963132456511033 1102 7 -0.008267998325962367 1102 8 -0.08009632685840273 1102 9 0.11284905604232165 1102 10-11 0.18540305187261197 1102 12-13 0.07738167308180266 1102 14-15 0.03269506166529368 1102 16-17 0.2365666919102054 1102 18-19 0.05624130316434872 1102 20-21 0.019389018021946924 1102 22-23 -0.12092080670193184 1102 24-25 -0.14604262753154984 1102 26-27 -0.028955179765489447 1102 28-29 -0.198160373482402 1102 30-31 -0.052163118047886314 1102 32-33 -0.04049140570678844 1102 34-35 -0.16668288628860495 1102 36-37 -0.025290510261157806 1102 38-39 0.08595580939267222 1102 40-41 -0.1676835982871765 1102 42-43 -0.04683992336786247 1102 44-45 0.03631990458873702 1102 46-47 0.1050400369026363 1102 48-49 0.07362599158171435 1102 50-51 0.20174443739717418 1102 52-53 0.2939596150894346 1102 54-55 0.11344994427424382 1102 56-57 0.3565070321605077 1102 58-59 0.012869337764378486 1102 60-61 0.10526825691032826 1102 62-63 0.032315145697431547 1102 64-65 0.17506849098506194 1102 66-67 -0.14540944411570678 1102 68-69 0.040232892257549224 1102 70-71 -0.03860933295815272 1102 72-73 0.015991020804428047 1102 74-75 0.16300466730692875 1102 76-77 -0.06063896018225279 1102 78-79 -0.10408238729011643 1102 80-81 0.0492135759331056 1102 82-83 0.2233995675492082 1102 84-85 0.02554609015740894 1102 86-87 -0.001544925387353402 1102 88-89 0.14900262001271614 1102 90-91 0.05839579635112102 1102 92-93 0.1213601427580322 1102 94-95 -0.04524089408846166 1102 96-97 -0.11034387746380148 1102 98-99 -0.08022942824812773 1102 100-101 -0.14087795489129107 1103 1 0.12209403290398768 1103 2 0.20344484469966417 1103 3 0.19360929055089926 1103 4 0.24112555918981116 1103 5 0.09118475965968287 1103 6 0.23914888924244337 1103 7 0.20863808656270777 1103 8 0.24082580827737843 1103 9 0.11998338665500086 1103 10-11 0.17792674556716292 1103 12-13 0.09549593113667498 1103 14-15 0.39120051924027877 1103 16-17 0.379274857500441 1103 18-19 0.33855043016259856 1103 20-21 0.27024818570138365 1103 22-23 0.3980715589535464 1103 24-25 0.3403814718942826 1103 26-27 0.35486892950323323 1103 28-29 0.36241254826794034 1103 30-31 0.34297208832325765 1103 32-33 0.30333176625607194 1103 34-35 0.4282213881197876 1103 36-37 0.32763932816633456 1103 38-39 0.34597391184631476 1103 40-41 0.26683507459577527 1103 42-43 0.34892250271798986 1103 44-45 0.3539112563799449 1103 46-47 0.32402534339988875 1103 48-49 0.3323167138862928 1103 50-51 0.268634248635756 1103 52-53 0.31199500085246257 1103 54-55 0.18773801172456217 1103 56-57 0.3455282205586343 1103 58-59 0.48140029170330934 1103 60-61 0.4088462987233257 1103 62-63 0.3854229900196202 1103 64-65 0.3459091639048921 1103 66-67 0.2833788014579639 1103 68-69 0.32401992039070393 1103 70-71 0.4097858713715894 1103 72-73 0.3018136940453928 1103 74-75 0.3414932510271953 1103 76-77 0.2758418063131245 1103 78-79 0.322037238074131 1103 80-81 0.19927507048022974 1103 82-83 0.34064413966144613 1103 84-85 0.2746214402699252 1103 86-87 0.31595140379164377 1103 88-89 0.20045589631368443 1103 90-91 0.3727991336298295 1103 92-93 0.44742700649384304 1103 94-95 0.4575653855789419 1103 96-97 0.4968819755461986 1103 98-99 0.47983191008134796 1103 100-101 0.7254747965301434 1104 1 0.1635341593106041 1104 2 0.09018540053542878 1104 3 0.09392338363472774 1104 4 0.1494312517618539 1104 5 0.11988270882918073 1104 6 0.04923263789958554 1104 7 0.0388860555366648 1104 8 0.16125532291130895 1104 9 0.2247119515295779 1104 10-11 0.2264653115530706 1104 12-13 0.019797823816425364 1104 14-15 -0.049541036792099646 1104 16-17 0.19203826398206303 1104 18-19 0.14946090829077718 1104 20-21 0.22452142602498526 1104 22-23 0.19254059275220925 1104 24-25 0.10746495839163117 1104 26-27 -0.0325261353385784 1104 28-29 0.01785336141924887 1104 30-31 0.19017691633787592 1104 32-33 -0.019821681287751858 1104 34-35 0.16500956727751515 1104 36-37 0.015187414542289446 1104 38-39 0.1746649508254876 1104 40-41 0.13669392861550733 1104 42-43 0.2305404347331219 1104 44-45 0.24236386529737786 1104 46-47 -0.004691697841835207 1104 48-49 0.014210911036158791 1104 50-51 -0.025366566339265262 1104 52-53 -0.038881132917424566 1104 54-55 0.19204316715397596 1104 56-57 0.1960337330382984 1104 58-59 0.03366804519331623 1104 60-61 0.10636215654152892 1104 62-63 -0.00809364857884276 1104 64-65 -0.16033226959628166 1104 66-67 -0.4304228433098203 1104 68-69 -0.29842045962702457 1104 70-71 -0.3435493640450531 1104 72-73 -0.098923381049147 1104 74-75 -0.174506332936609 1104 76-77 -0.04484445394886194 1104 78-79 -0.0335513605392741 1104 80-81 -0.18844947508570797 1104 82-83 -0.2146701528917916 1104 84-85 -0.166334723477366 1104 86-87 -0.13281185023835107 1104 88-89 -0.14664438835765736 1104 90-91 -0.09648435799672939 1104 92-93 -0.06536450380305325 1104 94-95 0.14081027560005666 1104 96-97 0.002977368942620018 1104 98-99 -0.02580381318049163 1104 100-101 0.03543957593122116 1105 1 0.13836798192478028 1105 2 0.18776081360422125 1105 3 0.15257759417093553 1105 4 0.16438412446868966 1105 5 0.2154443527732255 1105 6 0.08811636518068156 1105 7 0.12846279050476284 1105 8 0.22933288950842012 1105 9 0.13527250213625308 1105 10-11 0.24862969655821132 1105 12-13 0.23836171528612482 1105 14-15 0.29865018247200226 1105 16-17 0.37344185685686426 1105 18-19 0.24319986978848362 1105 20-21 0.26583170676038037 1105 22-23 0.3057922250900589 1105 24-25 0.23756728190669918 1105 26-27 0.32397352033435567 1105 28-29 0.34399243591163753 1105 30-31 0.38361012678207373 1105 32-33 0.20143078119495073 1105 34-35 0.10234964813500369 1105 36-37 0.10747207655262514 1105 38-39 0.3807255276524728 1105 40-41 0.4335222236919307 1105 42-43 0.4951275217756361 1105 44-45 0.2322081339832991 1105 46-47 0.2750408945946461 1105 48-49 0.32201524965609707 1105 50-51 0.13551085421567421 1105 52-53 0.35835931571698865 1105 54-55 0.2517959714901252 1105 56-57 0.3025997434057075 1105 58-59 0.32292120671931457 1105 60-61 0.35758937826738446 1105 62-63 0.25117294219734276 1105 64-65 0.3897463542234334 1105 66-67 0.3417841496957905 1105 68-69 0.2544868035961798 1105 70-71 0.16367426896166393 1105 72-73 0.39892364720051887 1105 74-75 0.43312283671076557 1105 76-77 0.3395514449970847 1105 78-79 0.48423645928664527 1105 80-81 0.24681413053315637 1105 82-83 0.2506027807253126 1105 84-85 0.1995510603663746 1105 86-87 0.37010299461163854 1105 88-89 0.42362232659395005 1105 90-91 0.29849205362055287 1105 92-93 0.322645530659333 1105 94-95 0.31522992866085886 1105 96-97 0.26764460107743915 1105 98-99 0.36844498489049116 1105 100-101 0.37100683003173174 1106 1 0.3135274581932528 1106 2 0.3608371229094729 1106 3 0.38919850855316795 1106 4 0.4022907066379702 1106 5 0.3493246953073381 1106 6 0.343012292346188 1106 7 0.35978387711287496 1106 8 0.4359869845427937 1106 9 0.4682145374667357 1106 10-11 0.3959756147212232 1106 12-13 0.520861793056433 1106 14-15 0.4228331397179943 1106 16-17 0.5221333023786627 1106 18-19 0.6732409311653527 1106 20-21 0.595687439132476 1106 22-23 0.4879409965278967 1106 24-25 0.34102172459791547 1106 26-27 0.37661412198432487 1106 28-29 0.250973220113778 1106 30-31 0.5157658537809482 1106 32-33 0.4955685293142267 1106 34-35 0.6508284945434255 1106 36-37 0.37989325684871034 1106 38-39 0.42655661484907625 1106 40-41 0.5458097575624166 1106 42-43 0.4557593156518607 1106 44-45 0.21111790332126645 1106 46-47 0.4757437092603922 1106 48-49 0.6020160388059068 1106 50-51 0.6584603002823286 1106 52-53 0.6473397812965302 1106 54-55 0.6602448302057766 1106 56-57 0.6153610903632369 1106 58-59 0.7435199424905434 1106 60-61 0.8440404436398268 1106 62-63 0.8204722135265783 1106 64-65 0.46224511629517195 1106 66-67 0.4595732428104853 1106 68-69 0.5686759595735253 1106 70-71 0.6450648101797256 1106 72-73 0.547319094210124 1106 74-75 0.7320232251313783 1106 76-77 0.6893740142237661 1106 78-79 0.6664372795984441 1106 80-81 0.731716523071082 1106 82-83 0.667460663597975 1106 84-85 0.7808734869131584 1106 86-87 0.8774084883736499 1106 88-89 0.9185030202054634 1106 90-91 0.7566155361736726 1106 92-93 0.8487543366420063 1106 94-95 0.9144162963537674 1106 96-97 1.0309238982977895 1106 98-99 1.1006708331019688 1106 100-101 1.529581838249797 1107 1 0.30149505462443926 1107 2 0.3382592221386602 1107 3 0.3172041877978806 1107 4 0.24243057270234658 1107 5 0.2773788075017478 1107 6 0.32788862243792494 1107 7 0.33394230647527223 1107 8 0.32270335200639266 1107 9 0.3113899793840744 1107 10-11 0.4259876029871208 1107 12-13 0.48193821610387744 1107 14-15 0.41964582210063384 1107 16-17 0.5224439693877656 1107 18-19 0.6882138220436929 1107 20-21 0.717563106566665 1107 22-23 0.6689746978627795 1107 24-25 0.6141339430017894 1107 26-27 0.6938475726527784 1107 28-29 0.5604333304698059 1107 30-31 0.7122181920556301 1107 32-33 0.5903047119268763 1107 34-35 0.4664307767869573 1107 36-37 0.6056259792455663 1107 38-39 0.6601165906888866 1107 40-41 0.6955905058944367 1107 42-43 0.6661656755054182 1107 44-45 0.6200601069642033 1107 46-47 0.609228695490323 1107 48-49 0.7873287245951488 1107 50-51 0.7555845087294486 1107 52-53 0.7501823362931077 1107 54-55 0.7136853321784926 1107 56-57 0.8462609567949357 1107 58-59 0.893224338573134 1107 60-61 1.0000644509791243 1107 62-63 0.8838008131685555 1107 64-65 0.8033409036357142 1107 66-67 0.9840181078958423 1107 68-69 0.9819927546097276 1107 70-71 0.8871566129844481 1107 72-73 0.9662855113656335 1107 74-75 1.0023217409502934 1107 76-77 0.9967544700029976 1107 78-79 1.041619791837764 1107 80-81 0.9917636940980508 1107 82-83 1.0399673664516094 1107 84-85 1.110557093855629 1107 86-87 1.215937847776054 1107 88-89 1.0668267809958607 1107 90-91 1.1337372178130813 1107 92-93 1.2695493568461025 1107 94-95 1.3079980714643256 1107 96-97 1.2829380128738528 1107 98-99 1.417240071039867 1107 100-101 1.6113048870906503 1108 1 -0.020576484947099516 1108 2 -0.06318294680350789 1108 3 0.014420582514276248 1108 4 0.04259651786829721 1108 5 0.04776786289080093 1108 6 0.11746793201723449 1108 7 0.05378801632097918 1108 8 0.0846516814547229 1108 9 0.11462091511501171 1108 10-11 -0.02771536071584535 1108 12-13 0.04301561093127049 1108 14-15 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-2.5448473865479286 2316 66-67 -2.1923430406230793 2316 68-69 -2.217318761407128 2316 70-71 -1.9982434342485433 2316 72-73 -2.2997457633663387 2316 74-75 -2.8048851666046453 2316 76-77 -2.877664356852023 2316 78-79 -3.061409429520925 2316 80-81 -2.9979631297355063 2316 82-83 -3.558549932181702 2316 84-85 -3.3344381017196127 2316 86-87 -3.7364000952811445 2316 88-89 -3.649973562254594 2316 90-91 -3.875979798864492 2316 92-93 -4.066861332232801 2316 94-95 -4.230362961676288 2316 96-97 -4.481806974028906 2316 98-99 -4.901721001401487 2316 100-101 -5.071597670521882 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4907.0 3 1879.0 4 1851.0 5 2106.0 6 2318.0 7 2740.0 8 3463.0 9 4290.0 10 5258.0 11 6547.0 12 7961.0 13 9556.0 14 11030.0 15 13121.0 16 16134.0 17 19969.0 18 18904.0 19 13249.0 20 13574.0 21 14887.0 22 16239.0 23 18613.0 24 21193.0 25 24699.0 26 29556.0 27 34678.0 28 41580.0 29 50416.0 30 61772.0 31 77325.0 32 97937.0 33 128139.0 34 174895.0 35 246131.0 36 368830.0 37 538336.0 38 469835.0 39 73081.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.381641562713526 18.83227314229739 14.54292110848415 23.243164186504934 2 15.159435876293648 20.062472154507105 40.69464561638726 24.083446352811986 3 19.236021577699212 24.440757305296636 29.342784031051572 26.98043708595258 4 12.95239045548882 16.692840709499873 37.315038338830824 33.03973049618048 5 14.295421673079286 36.89407360490069 33.890471823862505 14.920032898157515 6 31.92023939829695 35.85894956130232 17.87525690611287 14.34555413428786 7 28.71118785454515 32.213140439854286 20.81795010105966 18.2577216045409 8 26.082430606732853 35.06395686725731 19.898490285944614 18.95512224006522 9 26.1713894257379 17.273041408449853 20.276295772049373 36.27927339376287 10-11 24.708065803387562 26.728836070352298 27.622058115764997 20.94104001049515 12-13 25.7585778974121 23.917209100987773 28.000328910370982 22.32388409122915 14-15 22.764835101691794 25.370534953164046 25.993810902896726 25.87081904224743 16-17 22.38501038530522 28.694494370767682 26.454632564310458 22.46586267961664 18-19 22.346313489483315 27.367917681036698 28.57529523838326 21.71047359109673 20-21 22.976589589585426 26.55623038374989 28.615863406070886 21.851316620593796 22-23 22.719824437488356 26.490359669599638 28.510125946526294 22.279689946385712 24-25 22.587104638912244 26.68943904720219 28.48876188310031 22.234694430785257 26-27 22.510045990642443 26.92469053550849 28.273355216368717 22.29190825748035 28-29 22.29715217282479 26.899598294949552 28.534867488337518 22.268382043888142 30-31 22.488560150637277 26.61718213993187 28.522778447746937 22.37147926168391 32-33 22.241565933910245 26.848032854085318 28.42830453500475 22.482096676999685 34-35 22.67417950915533 26.820303640692288 28.254992594014272 22.250524256138107 36-37 22.638578589874925 26.591859915760708 28.208319692980126 22.56124180138424 38-39 22.49162472338211 26.83056109394761 28.327825374892214 22.34998880777807 40-41 22.71833410280493 26.723962705688827 28.23359419805821 22.32410899344803 42-43 22.529461567261308 26.700833382761797 28.16509962700086 22.604605422976036 44-45 22.58794323910081 26.864353600711453 28.005113785119022 22.54258937506871 46-47 22.670126202146708 26.949450194910547 27.769421305421815 22.611002297520933 48-49 22.725832000704187 27.113440268558303 27.520350219850755 22.640377510886758 50-51 22.646312355164802 27.19760375432475 27.507221453629693 22.648862436880755 52-53 22.92472139236286 27.18556935698519 27.34335965060586 22.546349600046092 54-55 22.719023141928812 27.04497448605135 27.426054321823546 22.809948050196294 56-57 22.67482146270987 27.378205113673715 27.38749940493867 22.559474018677747 58-59 22.856135745672944 27.101947152338347 27.380141456523898 22.66177564546481 60-61 22.79020417353617 26.951197674014455 27.74882846914427 22.5097696833051 62-63 22.745364215954172 27.091975534749942 27.677367301218897 22.48529294807699 64-65 22.65695083570666 27.099419842033374 27.496197040255556 22.74743228200441 66-67 22.585773783478018 27.34288504019365 27.33311719375251 22.738223982575825 68-69 22.428943635102293 27.227276240553678 27.57956984350129 22.764210280842747 70-71 22.534499700709322 27.164329011463767 27.483843311925902 22.817327975901005 72-73 22.627013485605136 27.114149259888848 27.36513780946896 22.893699445037058 74-75 22.49077946335277 27.353525094802965 27.310792207652362 22.8449032341919 76-77 22.59663189993378 27.26005333565091 27.305094848849258 22.838219915566054 78-79 22.625873753279055 27.278900459577788 27.201283885453957 22.8939419016892 80-81 22.505938051449565 27.39208719791079 27.274885704914997 22.827089045724648 82-83 22.567848712538595 27.427361533114397 27.294514342527332 22.710275411819676 84-85 22.63026151483265 27.306055287821223 27.168034095352883 22.89564910199324 86-87 22.620980564845226 27.123847645558648 27.35478619289056 22.900385596705565 88-89 22.667094524198426 27.0918293007194 27.206863258131325 23.03421291695085 90-91 22.551993718518183 27.211704267684855 27.374599494117724 22.861702519679238 92-93 22.684738475423572 27.16546310143774 27.303356263244606 22.846442159894085 94-95 22.575487139340066 27.112612197337338 27.2311193361031 23.0807813272195 96-97 22.551193157784066 27.109830291628466 27.207107162687393 23.13186938790007 98-99 22.390144801888287 27.10309866479314 27.167611270544967 23.3391452627736 100-101 22.374223008953948 27.132351523395837 27.30072715133088 23.192698316319333 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 319.0 1 246.0 2 209.5 3 241.5 4 221.5 5 242.5 6 407.5 7 815.0 8 1456.0 9 1874.0 10 1798.5 11 1644.5 12 1701.5 13 1888.5 14 2066.5 15 2224.5 16 2425.5 17 2684.0 18 2897.5 19 3046.0 20 3366.0 21 3890.5 22 4604.5 23 5648.0 24 7097.5 25 9141.5 26 11897.0 27 15277.5 28 18580.0 29 22387.5 30 27504.5 31 32773.5 32 37809.5 33 43203.5 34 50047.0 35 56155.5 36 62291.0 37 68646.5 38 72350.0 39 74964.0 40 77703.0 41 82606.5 42 87864.5 43 92497.5 44 97968.0 45 102293.0 46 103556.0 47 104596.5 48 108153.0 49 108448.0 50 105117.0 51 101650.5 52 97208.5 53 93652.0 54 90094.0 55 84023.0 56 77039.0 57 68989.5 58 61479.0 59 54284.5 60 45511.5 61 36568.5 62 28449.5 63 21772.5 64 16464.5 65 12511.0 66 9151.5 67 6794.0 68 4966.5 69 3260.0 70 2208.0 71 1486.0 72 968.0 73 600.0 74 422.0 75 307.0 76 179.5 77 90.0 78 51.0 79 37.0 80 24.5 81 19.0 82 16.5 83 9.0 84 8.0 85 8.0 86 8.5 87 6.0 88 4.5 89 4.5 90 4.0 91 4.0 92 3.0 93 2.0 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03653149509771514 2 0.02765362400364786 3 0.02247801404668098 4 0.04835606383151538 5 0.09467210622013872 6 0.11594121867103181 7 0.05908506549413286 8 0.08908093633961975 9 0.07430966996608654 10-11 0.07555634973674279 12-13 0.0730441011079961 14-15 0.06550735522175599 16-17 0.05538280435703247 18-19 0.06463845719978346 20-21 0.05984062899150029 22-23 0.034302582780481226 24-25 0.009727880028605633 26-27 0.003003364902035526 28-29 0.007121185962688008 30-31 0.0054022790061771095 32-33 0.006648958776833366 34-35 0.007007851438082894 36-37 0.006290066115583836 38-39 0.0039100410988764395 40-41 0.007291187749595679 42-43 0.004080042885784111 44-45 0.0025689158910492545 46-47 0.0017944633062476412 48-49 3.0222539894697115E-4 50-51 0.002153355967497169 52-53 0.006176731590978723 54-55 0.012901246717548831 56-57 0.009255652842750992 58-59 0.005288944481571995 60-61 0.0025311377161808834 62-63 0.009690101853737263 64-65 0.003003364902035526 66-67 0.02240245769694424 68-69 0.046334931476057516 70-71 0.060803972450643756 72-73 0.0036833720496662107 74-75 0.013505697515442774 76-77 0.02204356503569471 78-79 0.026029162484307894 80-81 0.0760663550974658 82-83 0.028616967462791333 84-85 0.012693466755772789 86-87 0.03296145757265404 88-89 0.04875273466763329 90-91 0.04376601558500826 92-93 0.04314267569968013 94-95 0.013411252078271844 96-97 0.0174346277017534 98-99 0.03915707825106695 100-101 0.0775208148298981 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2647031.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.77600696750529 #Duplication Level Percentage of deduplicated Percentage of total 1 78.15142648136306 45.15277360907709 2 12.434004626845569 14.367742759092453 3 3.99181390816478 6.918932045133384 4 1.771686274186108 4.094438340864402 5 0.9472917067273429 2.7365366124069466 6 0.568091215888805 1.9693225228422089 7 0.3877628614162655 1.568237284804816 8 0.27632724622502164 1.2772067922566717 9 0.20516581137585316 1.0668295212790653 >10 1.1369750533118486 12.05398735874691 >50 0.08576354181260866 3.365271341989516 >100 0.04205343668476352 4.215898479921401 >500 0.001310268798309068 0.47995130905475764 >1k 1.9654031974031608E-4 0.2294530627300049 >5k 1.310268798268774E-4 0.5034189598002418 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7839 0.29614311279316335 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5461 0.2063066129561762 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3393 0.12818134732838415 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 3.7778174868371394E-5 5 0.0 0.0 0.0 0.0 3.7778174868371394E-5 6 0.0 0.0 0.0 0.0 7.555634973674279E-5 7 0.0 0.0 0.0 0.0 7.555634973674279E-5 8 0.0 0.0 0.0 0.0 7.555634973674279E-5 9 0.0 0.0 0.0 0.0 7.555634973674279E-5 10-11 0.0 0.0 0.0 7.555634973674279E-5 7.555634973674279E-5 12-13 0.0 0.0 0.0 7.555634973674279E-5 7.555634973674279E-5 14-15 0.0 0.0 0.0 7.555634973674279E-5 9.44454371709285E-5 16-17 0.0 0.0 0.0 9.44454371709285E-5 1.1333452460511419E-4 18-19 0.0 0.0 0.0 1.1333452460511419E-4 1.5111269947348557E-4 20-21 0.0 0.0 0.0 2.0777996177604265E-4 1.5111269947348557E-4 22-23 0.0 0.0 0.0 3.2111448638115687E-4 2.2666904921022838E-4 24-25 0.0 0.0 0.0 6.23339885328128E-4 3.0222539894697115E-4 26-27 0.0 0.0 0.0 8.877871094067277E-4 3.0222539894697115E-4 28-29 0.0 0.0 0.0 0.00175668513137927 3.0222539894697115E-4 30-31 0.0 0.0 0.0 0.0039100410988764395 3.0222539894697115E-4 32-33 0.0 0.0 0.0 0.00636562246532058 3.0222539894697115E-4 34-35 0.0 1.8889087434185697E-5 0.0 0.009161207405580064 3.4000357381534254E-4 36-37 0.0 3.7778174868371394E-5 0.0 0.011220117935906304 3.4000357381534254E-4 38-39 0.0 3.7778174868371394E-5 0.0 0.013316806641100916 3.777817486837139E-4 40-41 0.0 3.7778174868371394E-5 0.0 0.015961278881886913 3.777817486837139E-4 42-43 0.0 3.7778174868371394E-5 0.0 0.01922909100800104 3.9667083611789964E-4 44-45 0.0 3.7778174868371394E-5 0.0 0.022591348571286092 4.1555992355208536E-4 46-47 0.0 3.7778174868371394E-5 0.0 0.026709169631938576 4.344490109862711E-4 48-49 0.0 3.7778174868371394E-5 0.0 0.03171477780199779 4.5333809842045675E-4 50-51 0.0 3.7778174868371394E-5 0.0 0.03930819095054043 4.5333809842045675E-4 52-53 0.0 3.7778174868371394E-5 0.0 0.056195035116702444 4.911162732888282E-4 54-55 0.0 3.7778174868371394E-5 0.0 0.07812526562779204 5.288944481571995E-4 56-57 0.0 3.7778174868371394E-5 0.0 0.10587333506861082 5.288944481571995E-4 58-59 0.0 3.7778174868371394E-5 0.0 0.1371914420344907 5.288944481571995E-4 60-61 0.0 3.7778174868371394E-5 0.0 0.18146746298022198 5.66672623025571E-4 62-63 0.0 3.7778174868371394E-5 0.0 0.21229445367281305 6.044507978939423E-4 64-65 0.0 3.7778174868371394E-5 0.0 0.24244143721777342 6.044507978939423E-4 66-67 0.0 3.7778174868371394E-5 0.0 0.30116761005065673 6.044507978939423E-4 68-69 0.0 3.7778174868371394E-5 0.0 0.37972732468943504 6.044507978939423E-4 70-71 0.0 3.7778174868371394E-5 0.0 0.4441013346651399 6.611180601964994E-4 72-73 0.0 3.7778174868371394E-5 0.0 0.5062086541487425 7.555634973674278E-4 74-75 0.0 3.7778174868371394E-5 0.0 0.6175976027481356 7.555634973674278E-4 76-77 0.0 3.7778174868371394E-5 0.0 0.7010307019449338 7.555634973674278E-4 78-79 0.0 3.7778174868371394E-5 0.0 0.7681058514237271 7.555634973674278E-4 80-81 0.0 3.7778174868371394E-5 0.0 0.8575645695120306 8.311198471041706E-4 82-83 0.0 3.7778174868371394E-5 0.0 1.0033883245039443 9.444543717092848E-4 84-85 0.0 3.7778174868371394E-5 0.0 1.1620755480385383 9.444543717092848E-4 86-87 0.0 3.7778174868371394E-5 0.0 1.3654354633549814 9.822325465776562E-4 88-89 0.0 3.7778174868371394E-5 0.0 1.6188892385468852 9.822325465776562E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4465 0.0 48.646713 1 GTATCAA 8275 0.0 41.64173 1 ATCAACG 10430 0.0 32.4498 3 TCAACGC 10485 0.0 32.280186 4 TATCAAC 10575 0.0 32.09415 2 CAACGCA 10685 0.0 31.808838 5 AACGCAG 11010 0.0 30.865799 6 ACGCAGA 12575 0.0 26.679739 7 CGCAGAG 12735 0.0 26.120647 8 GTACATG 9765 0.0 24.433756 1 GCAGAGT 13685 0.0 23.4366 9 TACATGG 10110 0.0 23.273523 2 GAGTACT 6950 0.0 22.254519 12-13 ACATGGG 10355 0.0 21.851091 3 GTACTTT 7470 0.0 20.484253 14-15 AGAGTAC 12225 0.0 20.210533 10-11 CATGGGG 5950 0.0 19.973194 4 CGCGGAA 240 0.0 19.794771 82-83 CAGAGTA 13095 0.0 19.684189 10-11 AGTACTT 7535 0.0 19.454674 12-13 >>END_MODULE