FastQCFastQC Report
Thu 26 May 2016
SRR936848_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936848_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2647031
Sequences flagged as poor quality0
Sequence length101
%GC45

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81680.30857213232485753No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62140.23475357863205984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41200.15564608045769013No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45500.050.6451
GTATCAA85950.043.7809871
ATCAACG108700.034.475793
TCAACGC109600.034.1926844
TATCAAC110000.033.9832762
CAACGCA111700.033.549855
AACGCAG115850.032.3504646
ACGCAGA135050.027.4698337
CGCAGAG135950.027.1153328
GCAGAGT148800.024.2629189
GTACATG94750.023.7686961
GAGTACT80200.022.5664112-13
TACATGG96850.022.4620322
GTGGTAT24600.021.8247641
ACATGGG96250.021.6142463
AGAGTAC130800.021.1190510-11
AGTACTT85350.020.84299912-13
GTACTTT88850.020.1538914-15
CAGAGTA140750.020.04797210-11
GTGTACG2803.483001E-818.6655541
Produced by FastQC (version 0.11.5)