##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936848_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2647031 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07914829860323 31.0 31.0 34.0 31.0 34.0 2 32.22188633227189 33.0 31.0 34.0 30.0 34.0 3 32.355794473128576 34.0 31.0 34.0 31.0 34.0 4 36.0288428809485 37.0 35.0 37.0 35.0 37.0 5 35.85877347110782 37.0 35.0 37.0 35.0 37.0 6 35.93802037074745 37.0 35.0 37.0 35.0 37.0 7 35.89534916667013 37.0 35.0 37.0 35.0 37.0 8 35.83928559960197 37.0 35.0 37.0 35.0 37.0 9 37.53514371384392 39.0 37.0 39.0 35.0 39.0 10-11 37.437159028360455 39.0 37.0 39.0 34.5 39.0 12-13 37.32731992938504 39.0 37.0 39.0 34.0 39.0 14-15 38.52776280292902 40.0 38.0 41.0 34.0 41.0 16-17 38.5228739293193 40.0 38.0 41.0 34.0 41.0 18-19 38.62658710834894 40.0 38.0 41.0 34.0 41.0 20-21 38.62041358790282 40.0 38.0 41.0 34.0 41.0 22-23 38.53196128039301 40.0 38.0 41.0 34.0 41.0 24-25 38.43831088491219 40.0 38.0 41.0 34.0 41.0 26-27 38.24614993175372 40.0 38.0 41.0 34.0 41.0 28-29 38.09122919225351 40.0 38.0 41.0 33.0 41.0 30-31 38.11540741306014 40.0 37.5 41.0 34.0 41.0 32-33 38.02070338428224 40.0 37.0 41.0 33.0 41.0 34-35 37.91485498280905 40.0 37.0 41.0 33.0 41.0 36-37 37.781894507468934 40.0 37.0 41.0 33.0 41.0 38-39 37.63839014352306 40.0 37.0 41.0 33.0 41.0 40-41 37.32179713800103 40.0 36.5 41.0 31.5 41.0 42-43 37.14712785758837 39.0 36.0 41.0 31.0 41.0 44-45 36.96160301862729 39.0 36.0 41.0 31.0 41.0 46-47 36.64747220565229 39.0 36.0 40.5 30.5 41.0 48-49 36.32792060236544 39.0 35.0 40.0 30.0 41.0 50-51 36.06509085084383 39.0 35.0 40.0 29.5 41.0 52-53 35.806385909345224 38.5 35.0 40.0 29.0 41.0 54-55 35.40755926167846 38.0 34.5 40.0 28.0 41.0 56-57 35.21083772724989 38.0 34.0 40.0 28.0 41.0 58-59 34.87683540540326 38.0 34.0 40.0 27.0 41.0 60-61 34.49266706736718 37.0 33.5 40.0 26.0 41.0 62-63 34.1061281488581 37.0 33.0 39.0 26.0 40.5 64-65 33.77340575157601 36.0 33.0 39.0 26.0 40.0 66-67 33.421025292110286 36.0 32.5 39.0 25.0 40.0 68-69 32.95937335074656 35.5 32.0 38.5 23.5 40.0 70-71 32.509677446165156 35.0 31.0 38.0 23.0 40.0 72-73 32.08110350804354 35.0 31.0 37.0 22.0 39.0 74-75 31.586988969906283 34.5 31.0 36.5 21.5 39.0 76-77 30.16669884107893 33.5 29.5 35.0 20.0 37.5 78-79 30.860974994248274 34.0 30.0 36.0 20.0 37.5 80-81 30.723865530853246 34.0 30.0 35.0 20.0 37.0 82-83 30.364444164046436 34.0 30.0 35.0 18.0 37.0 84-85 29.905437639377855 34.0 30.0 35.0 16.0 36.0 86-87 29.50904163948212 34.0 29.0 35.0 11.0 36.0 88-89 29.113406680919113 34.0 29.0 35.0 7.0 35.5 90-91 28.63182694120318 33.0 29.0 35.0 2.0 35.0 92-93 28.104777201324808 33.0 27.5 35.0 2.0 35.0 94-95 27.497074835919943 33.0 26.5 34.0 2.0 35.0 96-97 26.65690144920857 32.0 25.0 34.0 2.0 35.0 98-99 25.69594632628027 32.0 24.0 34.0 2.0 35.0 100-101 22.33423862433043 28.0 8.5 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13895992916283717 1101 2 0.26275237772797766 1101 3 0.2357560732655486 1101 4 0.1637938285839624 1101 5 0.005127749557779282 1101 6 0.046875200731193445 1101 7 0.0939842598052607 1101 8 0.15331219072736246 1101 9 0.1660643601386127 1101 10-11 0.27778149347896886 1101 12-13 0.2942472987072122 1101 14-15 0.3970903281814273 1101 16-17 0.40381157630577746 1101 18-19 0.23477004426924708 1101 20-21 0.15208090073230096 1101 22-23 0.1747698070061432 1101 24-25 0.20911575837221363 1101 26-27 0.20335380064555864 1101 28-29 0.25541249967918134 1101 30-31 0.2804085115414381 1101 32-33 0.27978746037971547 1101 34-35 0.06324790618617016 1101 36-37 0.30346169899927133 1101 38-39 0.30202801955822167 1101 40-41 0.36060378086456524 1101 42-43 0.2867292523236529 1101 44-45 0.15335863181682186 1101 46-47 0.025339919669029598 1101 48-49 0.18392339536926983 1101 50-51 0.28938597649027287 1101 52-53 0.21122439104760105 1101 54-55 0.037844211529268534 1101 56-57 0.18922437360233602 1101 58-59 0.22035048718886685 1101 60-61 0.35149478421985236 1101 62-63 0.3595110607762848 1101 64-65 0.2927952070942652 1101 66-67 0.16131183133371962 1101 68-69 -0.14698383009672256 1101 70-71 -0.16664288532384575 1101 72-73 -0.011811519140593418 1101 74-75 -0.03247568814083479 1101 76-77 0.11627337287361783 1101 78-79 0.22037357182984962 1101 80-81 0.20746460640327413 1101 82-83 0.45655754428358364 1101 84-85 0.16381572635877362 1101 86-87 -0.147764555305546 1101 88-89 -0.02189059253948855 1101 90-91 0.20855148624124453 1101 92-93 0.15486113178827665 1101 94-95 -0.04767036221135257 1101 96-97 0.018268946894071547 1101 98-99 0.030389955827999415 1101 100-101 0.4371498207551525 1102 1 -0.08337565413943793 1102 2 1.865843573725101E-4 1102 3 0.07096155041359253 1102 4 0.08004791427518398 1102 5 -0.130329383500289 1102 6 0.08787885539707929 1102 7 0.031558164228172814 1102 8 0.15025424478462668 1102 9 0.1864778860831322 1102 10-11 0.1975468543251182 1102 12-13 0.20799969454508727 1102 14-15 0.15572015876707468 1102 16-17 0.07732234200617683 1102 18-19 0.1675110168691205 1102 20-21 0.10993926116137942 1102 22-23 0.046247593204405746 1102 24-25 0.07139313710436568 1102 26-27 0.08795132060142663 1102 28-29 -0.0978474120754953 1102 30-31 -0.058505075464502454 1102 32-33 -0.16567756556712254 1102 34-35 0.11268766465109792 1102 36-37 0.14705940348005697 1102 38-39 0.31230864124874813 1102 40-41 0.24784967213819442 1102 42-43 0.2772140921513966 1102 44-45 0.20131061243707649 1102 46-47 0.15311824454843048 1102 48-49 0.16815632626602905 1102 50-51 0.0824493874906409 1102 52-53 0.06852032830620658 1102 54-55 0.2164519600644681 1102 56-57 0.09787905533098495 1102 58-59 0.025331843347068173 1102 60-61 0.18514459687952467 1102 62-63 0.05849766065972517 1102 64-65 0.11144950612261795 1102 66-67 0.07623792264503493 1102 68-69 0.05945768610819613 1102 70-71 -0.165891988354808 1102 72-73 -0.2705403155335553 1102 74-75 -0.230885373432006 1102 76-77 -0.14751876772998074 1102 78-79 -0.5663590744792728 1102 80-81 -0.1817500584768048 1102 82-83 -0.07180164407003886 1102 84-85 -0.01090718766261034 1102 86-87 -0.09380186443987171 1102 88-89 -0.11634565535961627 1102 90-91 -0.09982200827797527 1102 92-93 -0.23012701059670704 1102 94-95 -0.02434794843043875 1102 96-97 0.10947989348739284 1102 98-99 0.07083912165105843 1102 100-101 0.1797237288344462 1103 1 0.13010221692650248 1103 2 0.19018588313982576 1103 3 0.16140686818905436 1103 4 0.10632149036604233 1103 5 0.015806114986105513 1103 6 0.032048313720032695 1103 7 0.10749556112305925 1103 8 0.19393140610565496 1103 9 0.14813679061218465 1103 10-11 0.1465377411985358 1103 12-13 0.21381580664498756 1103 14-15 0.2264907451693574 1103 16-17 0.1856098289846102 1103 18-19 0.14182766157723137 1103 20-21 0.12663399414842758 1103 22-23 0.15726354467028614 1103 24-25 0.23308051318002043 1103 26-27 0.11974078901862129 1103 28-29 0.14108624158622973 1103 30-31 0.14270113617188684 1103 32-33 0.1440905710371041 1103 34-35 0.12896800978167988 1103 36-37 0.028784206014499603 1103 38-39 0.08345883652667396 1103 40-41 0.19448525501734792 1103 42-43 0.03853725794776608 1103 44-45 -0.012789603883256007 1103 46-47 -0.06503441015901501 1103 48-49 0.009125237427873856 1103 50-51 -0.04161191184315527 1103 52-53 -0.06398429978833065 1103 54-55 0.19581143953318048 1103 56-57 -0.03697200876370488 1103 58-59 -0.1091850559370755 1103 60-61 -0.3553592907668559 1103 62-63 0.012088217502807197 1103 64-65 -0.10547026249203384 1103 66-67 -0.08000233210483287 1103 68-69 -0.1557503247326011 1103 70-71 -0.08871847530306098 1103 72-73 0.03252250169543913 1103 74-75 -0.11156492723213063 1103 76-77 0.06835305064222297 1103 78-79 -0.021326402087524343 1103 80-81 -0.07483382179039566 1103 82-83 0.18931133264777245 1103 84-85 -0.090639498745265 1103 86-87 -0.313745031756131 1103 88-89 0.03731577496208516 1103 90-91 -0.24022473031757485 1103 92-93 -0.21373690201604845 1103 94-95 0.11649077353069615 1103 96-97 0.2166856026432633 1103 98-99 0.06822282847963734 1103 100-101 0.6179814651609092 1104 1 0.051507706894618366 1104 2 0.13756264666313456 1104 3 0.07223549261949813 1104 4 0.054753660792705716 1104 5 -0.16778624362569872 1104 6 0.05682918232383116 1104 7 0.09967101726686423 1104 8 0.11142302126647508 1104 9 0.05343655039402506 1104 10-11 0.1152509640258117 1104 12-13 0.18276304983523772 1104 14-15 0.13651454614447545 1104 16-17 0.09739590658961816 1104 18-19 0.17744452785579057 1104 20-21 0.200059574960612 1104 22-23 0.13275213786952378 1104 24-25 -0.02661387831004447 1104 26-27 0.007813515566013507 1104 28-29 0.06634808080818999 1104 30-31 0.03281895847883476 1104 32-33 0.017269522169947038 1104 34-35 0.03215357190072865 1104 36-37 -0.08056777485924016 1104 38-39 -0.01789477714666532 1104 40-41 0.16283070611742545 1104 42-43 0.01791051793334475 1104 44-45 0.17030247832094147 1104 46-47 -0.003336205953630156 1104 48-49 0.07991800010771755 1104 50-51 0.10159448058156784 1104 52-53 0.03381377162742183 1104 54-55 0.15909357334295748 1104 56-57 0.1857074495913551 1104 58-59 -0.14068875609464726 1104 60-61 -0.11668559137866197 1104 62-63 0.03168769004868466 1104 64-65 0.07857072419765387 1104 66-67 -0.08793355647657819 1104 68-69 -0.0472215113761294 1104 70-71 -0.016834686182122027 1104 72-73 -0.11590660545097364 1104 74-75 -0.14262836990319627 1104 76-77 0.05256771819795247 1104 78-79 0.2767254229151135 1104 80-81 0.1451414012591492 1104 82-83 0.14300539928765588 1104 84-85 0.05529214451224007 1104 86-87 -0.02646776072564805 1104 88-89 -0.0209583230799808 1104 90-91 0.017395713587358586 1104 92-93 -0.01948878059046777 1104 94-95 0.005618194590677916 1104 96-97 0.2654410916140293 1104 98-99 0.1909748794526216 1104 100-101 0.9566981573311608 1105 1 0.007971359116368149 1105 2 0.09960862718887142 1105 3 0.1503789365035857 1105 4 0.05849549662983122 1105 5 -0.013485915228713452 1105 6 -0.005168859563362105 1105 7 0.08932525056032148 1105 8 0.0627836078889672 1105 9 0.08255816018240836 1105 10-11 -0.0067076039298044066 1105 12-13 0.12367125698953174 1105 14-15 0.15123446625664627 1105 16-17 -0.0663692109021028 1105 18-19 -0.020189373815092893 1105 20-21 0.07197800852973302 1105 22-23 0.0479475607931974 1105 24-25 0.015145487039767147 1105 26-27 0.06230302595865567 1105 28-29 -0.12589779493526265 1105 30-31 0.09267256258385004 1105 32-33 0.07666043678376155 1105 34-35 0.09820172376479519 1105 36-37 0.14719864078508493 1105 38-39 0.017315262361890404 1105 40-41 0.04528710619776888 1105 42-43 0.14756109769898984 1105 44-45 0.11074855254717164 1105 46-47 0.0629563389696699 1105 48-49 0.1228630304688565 1105 50-51 0.10249234060865575 1105 52-53 0.3068627828249788 1105 54-55 0.4200370270225591 1105 56-57 0.20210441812798052 1105 58-59 0.22617651703685482 1105 60-61 0.05786695659213592 1105 62-63 0.2014497753844111 1105 64-65 0.10161027382181231 1105 66-67 0.12785048762498974 1105 68-69 0.2139529160069671 1105 70-71 0.04521122013519374 1105 72-73 -0.008727015140813421 1105 74-75 -0.09550095305867501 1105 76-77 -0.10053853490024878 1105 78-79 0.15765343876998372 1105 80-81 -0.015312660266555866 1105 82-83 -0.10467639154948571 1105 84-85 -0.3839241725338276 1105 86-87 -0.1941807660973467 1105 88-89 0.15548771453676125 1105 90-91 0.2712463884387155 1105 92-93 0.19524001979580774 1105 94-95 0.37870312266293027 1105 96-97 0.4877607948397191 1105 98-99 0.6579966581393712 1105 100-101 0.8912263575197521 1106 1 0.031155799452264432 1106 2 0.12677585005352654 1106 3 0.18519337115787948 1106 4 0.1633499395634601 1106 5 0.04944662741063155 1106 6 0.136198844790421 1106 7 0.15964348128105854 1106 8 0.21556826304598786 1106 9 0.23552246138823563 1106 10-11 0.2474861684392593 1106 12-13 0.3651144692440269 1106 14-15 0.40976041521265216 1106 16-17 0.3500419135245991 1106 18-19 0.338414911052908 1106 20-21 0.19700128912244708 1106 22-23 0.311500905771652 1106 24-25 0.3155887576051697 1106 26-27 0.31783310098309414 1106 28-29 0.3594506153790107 1106 30-31 0.38373491578817465 1106 32-33 0.35133911073736357 1106 34-35 0.37744339389129067 1106 36-37 0.3859357155952736 1106 38-39 0.43571333403528456 1106 40-41 0.5119963636118356 1106 42-43 0.6826074966104443 1106 44-45 0.38947687129581965 1106 46-47 0.36586832870049335 1106 48-49 0.31728025343451094 1106 50-51 0.5165201248766422 1106 52-53 0.46268007605998207 1106 54-55 0.5732423151881534 1106 56-57 0.49323040158572695 1106 58-59 0.576173951626636 1106 60-61 0.42354887831466925 1106 62-63 0.3641463406839307 1106 64-65 0.4232129939915765 1106 66-67 0.47427183463907596 1106 68-69 0.38484344194030484 1106 70-71 0.5708268748204759 1106 72-73 0.47756076624798993 1106 74-75 0.3234155200126665 1106 76-77 0.522626338291051 1106 78-79 0.5845543303072702 1106 80-81 0.5885010745222807 1106 82-83 0.6239797657784365 1106 84-85 0.5103407956921195 1106 86-87 0.5817769096167567 1106 88-89 0.7505455224536561 1106 90-91 0.6307658042847102 1106 92-93 0.7690595304609822 1106 94-95 0.9728602763261556 1106 96-97 0.832977341722156 1106 98-99 0.9361797093830688 1106 100-101 1.3302444019822843 1107 1 0.23604702378687392 1107 2 0.3242248406782693 1107 3 0.23125947517289092 1107 4 0.1281688077372678 1107 5 0.10873951819417726 1107 6 0.12449599263769784 1107 7 0.13026215815212083 1107 8 0.15642866921329812 1107 9 0.3016572839088738 1107 10-11 0.3230186864994167 1107 12-13 0.30853678590309386 1107 14-15 0.31810787112015504 1107 16-17 0.31256962176332337 1107 18-19 0.37303202859034457 1107 20-21 0.3410790946565996 1107 22-23 0.2850667350370628 1107 24-25 0.30095030587178684 1107 26-27 0.34191242319433 1107 28-29 0.4490915628163705 1107 30-31 0.35322526785572705 1107 32-33 0.3358516753109271 1107 34-35 0.3803433722964158 1107 36-37 0.29009261050702406 1107 38-39 0.41939587337927264 1107 40-41 0.4920305095819373 1107 42-43 0.4520364410298612 1107 44-45 0.3700320787858331 1107 46-47 0.1853448025803175 1107 48-49 0.226729521919232 1107 50-51 0.06927590046886678 1107 52-53 0.2082969681694422 1107 54-55 0.3660651148733294 1107 56-57 0.15583780942760228 1107 58-59 0.10672314572902053 1107 60-61 0.07990539932997365 1107 62-63 0.09623629503846587 1107 64-65 0.06172441691017383 1107 66-67 0.1799990827795881 1107 68-69 0.016578250953038776 1107 70-71 -0.006813661455218778 1107 72-73 0.32333144408119097 1107 74-75 0.3176948036552716 1107 76-77 0.032130230848704144 1107 78-79 0.35914708621912794 1107 80-81 0.2807712942419833 1107 82-83 0.41699489018891 1107 84-85 0.42799093265220733 1107 86-87 0.715718157930862 1107 88-89 0.6783933873011421 1107 90-91 0.48225438432838175 1107 92-93 0.7719334409788097 1107 94-95 0.9591981114058861 1107 96-97 0.8194528478316663 1107 98-99 1.083587169123735 1107 100-101 1.3966222038728588 1108 1 0.06870658644643868 1108 2 0.17690438335781522 1108 3 0.15077190718532307 1108 4 0.07817102773948648 1108 5 -0.03611257415791158 1108 6 0.08355887420058394 1108 7 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-1.1482742559455446 2316 88-89 -1.8893255655686225 2316 90-91 -1.8300390839975726 2316 92-93 -1.6847284843606083 2316 94-95 -1.9097348375734633 2316 96-97 -1.7077683197607278 2316 98-99 -2.216141331300822 2316 100-101 -2.697821249039272 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 9.0 4 21.0 5 57.0 6 117.0 7 281.0 8 648.0 9 1277.0 10 2104.0 11 3374.0 12 5133.0 13 6934.0 14 9143.0 15 12416.0 16 15944.0 17 21111.0 18 19295.0 19 13441.0 20 12925.0 21 13729.0 22 15111.0 23 16956.0 24 19908.0 25 23868.0 26 29227.0 27 36931.0 28 45751.0 29 57713.0 30 72090.0 31 90095.0 32 114721.0 33 148927.0 34 201610.0 35 284005.0 36 427462.0 37 584743.0 38 326867.0 39 13086.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.238994224012785 19.55258203934745 15.215792786930837 22.992630949708932 2 15.718323246877821 20.69696091613401 39.73695753110468 23.84775830588349 3 19.522429356461714 24.456502439345044 29.709008840878543 26.3120593633147 4 13.580498301682148 17.324768769236172 36.81464251835358 32.28009041072809 5 14.297517538921861 37.016135430322215 33.89705965689071 14.789287373865212 6 32.083115006964405 36.2424920599721 17.475617021485583 14.198775911577915 7 28.8080014355739 32.18847574163448 20.7724520924434 18.231070730348222 8 26.431613381180654 35.09592445271703 19.702262648227393 18.770199517874932 9 26.167847929016215 17.066580733781713 20.1900849519102 36.575486385291875 10-11 24.77573930943763 26.81200182393028 27.503871318469635 20.90838754816245 12-13 25.840199934261975 23.88285030998878 28.058385942641685 22.218563813107558 14-15 22.792716059807027 25.340444168403103 26.060009146388115 25.806830625401762 16-17 22.388819775816753 28.688745995041238 26.563591435083307 22.358842794058702 18-19 22.284308823565524 27.200517412130885 28.88628134397952 21.628892420324068 20-21 22.836831472580776 26.398558369325304 29.045306908474778 21.719303249619145 22-23 22.68568776502976 26.348092295760033 28.86974849361945 22.09647144559076 24-25 22.60291244548822 26.54755660092417 28.89723706228836 21.952293891299252 26-27 22.59097141641763 26.769206881759587 28.612312721463123 22.027508980359663 28-29 22.29016844551701 26.646733619742598 28.966372852875 22.096725081865397 30-31 22.51654778504672 26.41629433127153 28.921950668503694 22.14520721517806 32-33 22.337282102282654 26.662699953060553 28.790048460017886 22.209969484638904 34-35 22.685510672145508 26.619862026549747 28.668629116923828 22.025998184380917 36-37 22.669559519354767 26.37549497380698 28.63059025490714 22.324355251931113 38-39 22.501900428144168 26.621106767035467 28.780945241977772 22.09604756284259 40-41 22.722334917319113 26.465060920520077 28.669450054013684 22.143154108147122 42-43 22.58077068232295 26.5427567716434 28.510810791411206 22.365661754622444 44-45 22.66619770907163 26.68407753591747 28.306949116652763 22.342775638358138 46-47 22.78346875897824 26.788985141618447 28.011886554128985 22.415659545274323 48-49 22.860846201853548 26.96898181658827 27.763816627553616 22.406355354004567 50-51 22.741383120795923 27.044769804135647 27.707807184400362 22.506039890668063 52-53 23.01657941124692 27.095395648990678 27.48783725686792 22.400187682894487 54-55 22.84034452184353 26.939087604187485 27.614939152582647 22.60562872138634 56-57 22.787935203446438 27.247585826117525 27.533718533288425 22.43076043714761 58-59 22.97165839260423 26.9872011127956 27.502023968777046 22.539116525823122 60-61 22.926030748725477 26.879737843796203 27.897108072376298 22.29712333510202 62-63 22.811358390346918 26.937718877383332 27.86096115268811 22.389961579581644 64-65 22.74514611384048 26.947292029534246 27.72471093529971 22.582850921325555 66-67 22.69109152521883 27.20562290567164 27.557054510149108 22.54623105896042 68-69 22.587818756012638 27.020166197681668 27.78200159159601 22.610013454709687 70-71 22.65853751139727 26.94130427267993 27.721390373422444 22.678767842500356 72-73 22.721150062872578 26.97991949816785 27.545857691695247 22.75307274726433 74-75 22.696463706179788 27.127635861927228 27.426337916214578 22.74956251567841 76-77 22.709092832344176 27.119054185256687 27.45928455665406 22.71256842574508 78-79 22.712275613618235 27.095706896554983 27.387581792310574 22.80443569751621 80-81 22.63332423490566 27.261451976830177 27.392526003715556 22.712697784548602 82-83 22.660187198624268 27.247350613593813 27.44817961880267 22.64428256897925 84-85 22.70880045637173 27.193464968369437 27.308899059866675 22.788835515392154 86-87 22.661271418185088 26.959065862920216 27.502318172450874 22.877344546443823 88-89 22.717979057508547 26.968170692223502 27.339539691336828 22.974310558931123 90-91 22.635311584751374 26.97695592261784 27.566832924536055 22.820899568094735 92-93 22.659594844185808 26.957731133485023 27.466433147175078 22.916240875154088 94-95 22.563373832796067 26.85427560160799 27.378957783267367 23.203392782328578 96-97 22.625914493802384 26.898126556818795 27.331122171328598 23.144836778050223 98-99 22.438037728549983 26.833783274285665 27.27643299204311 23.451746005121244 100-101 22.166760484629595 27.13744394534128 27.33359274944559 23.362202820583537 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 447.0 1 314.5 2 220.0 3 268.5 4 247.0 5 222.5 6 396.5 7 870.5 8 1492.0 9 1899.0 10 1809.0 11 1698.5 12 1761.0 13 1911.0 14 2172.0 15 2399.0 16 2642.5 17 2839.5 18 3070.0 19 3360.0 20 3649.5 21 4127.0 22 4861.0 23 5966.5 24 7410.0 25 9320.5 26 12021.5 27 15444.5 28 18779.5 29 22311.0 30 27334.0 31 32817.5 32 37773.5 33 43008.5 34 50021.0 35 55965.0 36 61515.5 37 68138.5 38 72384.5 39 74711.5 40 77567.0 41 82822.5 42 88010.0 43 92856.0 44 98227.5 45 102319.5 46 103717.5 47 105063.0 48 108645.0 49 109065.0 50 105541.5 51 101541.0 52 97233.0 53 94167.0 54 90616.0 55 84074.0 56 76562.5 57 68194.0 58 60894.0 59 53809.0 60 45429.5 61 36421.5 62 28004.5 63 21586.0 64 16193.0 65 11917.0 66 8708.5 67 6662.0 68 4862.0 69 3120.5 70 2001.0 71 1301.5 72 872.5 73 590.0 74 392.0 75 270.0 76 172.5 77 92.0 78 46.0 79 26.0 80 19.0 81 12.0 82 10.5 83 7.0 84 3.5 85 2.5 86 1.5 87 1.5 88 1.0 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02104244340168287 2 0.0030978103392064545 3 3.4000357381534254E-4 4 0.0 5 0.002304468666970655 6 0.0 7 2.2666904921022835E-4 8 0.0 9 1.5111269947348557E-4 10-11 0.0 12-13 0.006082286153807795 14-15 0.003192255776377383 16-17 0.0 18-19 4.34449010986271E-4 20-21 0.0010766779837485846 22-23 0.0015300160821690417 24-25 4.34449010986271E-4 26-27 1.7000178690767127E-4 28-29 1.1333452460511417E-4 30-31 0.0 32-33 1.3222361203929988E-4 34-35 0.0 36-37 0.0017944633062476412 38-39 0.009784547290908191 40-41 6.233398853281281E-4 42-43 0.0 44-45 9.633434591434706E-4 46-47 2.4555813664441404E-4 48-49 1.1333452460511417E-4 50-51 0.001737796043945084 52-53 4.911162732888281E-4 54-55 0.0 56-57 2.8333631151278543E-4 58-59 1.5111269947348557E-4 60-61 0.002247801404668098 62-63 3.7778174868371394E-5 64-65 9.444543717092848E-5 66-67 3.7778174868371394E-5 68-69 9.444543717092848E-5 70-71 5.855617104597566E-4 72-73 3.9667083611789964E-4 74-75 0.0029844758146013403 76-77 3.7778174868371394E-5 78-79 2.4555813664441404E-4 80-81 0.0020211323554578694 82-83 1.1333452460511417E-4 84-85 0.0024933595413125123 86-87 3.9667083611789964E-4 88-89 0.0025689158910492545 90-91 0.0014544597324322986 92-93 0.0 94-95 0.0 96-97 2.4555813664441404E-4 98-99 6.422289727623136E-4 100-101 0.002304468666970655 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2647031.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.168944303685784 #Duplication Level Percentage of deduplicated Percentage of total 1 77.01637503673881 43.25928479910358 2 13.092032654973954 14.707313060385353 3 4.167708798410765 7.0228741011574645 4 1.817612521250283 4.083735058871562 5 0.9976272943880825 2.8017835967160476 6 0.6177353781389084 2.081852642946038 7 0.4181299307322146 1.6440141753701232 8 0.28893553365089303 1.2983363117594193 9 0.22564082097147786 1.140660603520639 >10 1.2195494242394163 12.60747828589308 >50 0.09258436854411764 3.5573997407605766 >100 0.044111390609794375 4.380370590367392 >500 0.0014845048871513617 0.5194007377901584 >1k 3.3738747434689957E-4 0.35039841074146255 >5k 1.3495498973875982E-4 0.5450978846169513 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8168 0.30857213232485753 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6214 0.23475357863205984 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4120 0.15564608045769013 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.7778174868371394E-5 0.0 8 0.0 0.0 0.0 3.7778174868371394E-5 0.0 9 0.0 0.0 0.0 7.555634973674279E-5 1.1333452460511419E-4 10-11 0.0 0.0 0.0 7.555634973674279E-5 1.1333452460511419E-4 12-13 0.0 0.0 0.0 7.555634973674279E-5 1.1333452460511419E-4 14-15 0.0 0.0 0.0 1.1333452460511419E-4 1.3222361203929988E-4 16-17 0.0 0.0 0.0 1.7000178690767127E-4 1.5111269947348557E-4 18-19 0.0 0.0 0.0 1.8889087434185696E-4 1.5111269947348557E-4 20-21 0.0 0.0 0.0 2.4555813664441404E-4 1.5111269947348557E-4 22-23 0.0 0.0 0.0 3.4000357381534254E-4 1.88890874341857E-4 24-25 0.0 0.0 0.0 6.23339885328128E-4 2.2666904921022838E-4 26-27 0.0 0.0 0.0 8.688980219725422E-4 2.2666904921022838E-4 28-29 0.0 0.0 0.0 0.0017377960439450841 2.455581366444141E-4 30-31 0.0 0.0 0.0 0.0039100410988764395 2.6444722407859976E-4 32-33 0.0 0.0 0.0 0.0064222897276231365 2.6444722407859976E-4 34-35 0.0 0.0 0.0 0.009274541930185176 3.0222539894697115E-4 36-37 0.0 0.0 0.0 0.011409008810248162 3.2111448638115687E-4 38-39 0.0 0.0 0.0 0.013581253865179516 3.588926612495282E-4 40-41 0.0 0.0 0.0 0.01616905884366296 3.9667083611789964E-4 42-43 0.0 0.0 0.0 0.01939909279490871 4.1555992355208536E-4 44-45 0.0 0.0 0.0 0.02264801583358865 4.1555992355208536E-4 46-47 0.0 0.0 0.0 0.026671391457070204 4.5333809842045675E-4 48-49 0.0 0.0 0.0 0.03163922145226104 4.911162732888281E-4 50-51 0.0 0.0 0.0 0.038949298289290904 4.911162732888281E-4 52-53 0.0 0.0 0.0 0.05562836249367688 6.044507978939423E-4 54-55 0.0 0.0 0.0 0.0775208148298981 7.366744099332422E-4 56-57 0.0 0.0 0.0 0.10504221522150667 7.555634973674278E-4 58-59 0.0 0.0 0.0 0.13636032218738653 7.555634973674278E-4 60-61 0.0 0.0 0.0 0.18065523222055202 7.555634973674278E-4 62-63 0.0 0.0 0.0 0.21152000108801144 7.555634973674278E-4 64-65 0.0 0.0 0.0 0.24119475744711716 7.555634973674278E-4 66-67 0.0 0.0 0.0 0.29931647948210655 7.555634973674278E-4 68-69 0.0 0.0 0.0 0.37778174868371395 7.555634973674278E-4 70-71 0.0 0.0 0.0 0.4416835314735642 7.555634973674278E-4 72-73 0.0 0.0 0.0 0.5033186237713121 7.933416722357993E-4 74-75 0.0 0.0 0.0 0.6148397959827445 7.933416722357993E-4 76-77 0.0 0.0 0.0 0.6982540060921085 7.933416722357993E-4 78-79 0.0 0.0 0.0 0.7648002611227447 7.933416722357993E-4 80-81 0.0 0.0 0.0 0.8540134210744037 7.933416722357993E-4 82-83 0.0 0.0 0.0 1.0000638451155277 8.122307596699851E-4 84-85 0.0 0.0 0.0 1.1578632815407148 8.500089345383564E-4 86-87 0.0 0.0 0.0 1.3606376351466984 8.688980219725421E-4 88-89 0.0 0.0 0.0 1.6113902708355134 8.877871094067277E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4550 0.0 50.645 1 GTATCAA 8595 0.0 43.780987 1 ATCAACG 10870 0.0 34.47579 3 TCAACGC 10960 0.0 34.192684 4 TATCAAC 11000 0.0 33.983276 2 CAACGCA 11170 0.0 33.54985 5 AACGCAG 11585 0.0 32.350464 6 ACGCAGA 13505 0.0 27.469833 7 CGCAGAG 13595 0.0 27.115332 8 GCAGAGT 14880 0.0 24.262918 9 GTACATG 9475 0.0 23.768696 1 GAGTACT 8020 0.0 22.56641 12-13 TACATGG 9685 0.0 22.462032 2 GTGGTAT 2460 0.0 21.824764 1 ACATGGG 9625 0.0 21.614246 3 AGAGTAC 13080 0.0 21.11905 10-11 AGTACTT 8535 0.0 20.842999 12-13 GTACTTT 8885 0.0 20.15389 14-15 CAGAGTA 14075 0.0 20.047972 10-11 GTGTACG 280 3.483001E-8 18.665554 1 >>END_MODULE