FastQCFastQC Report
Thu 26 May 2016
SRR936847_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936847_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309321
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66910.5110282352455968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49130.3752326587597694No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30490.2328687922976871No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45800.041.9324071
GTATCAA71000.034.8829571
ATCAACG88300.028.0410753
TATCAAC88250.027.9567432
TCAACGC90100.027.4808734
CAACGCA90300.027.4147645
AACGCAG93000.026.5677596
GAGTACT51850.023.32375712-13
ACGCAGA105000.023.305187
CGCAGAG104800.023.1700738
GTACATG64550.022.9033011
TACATGG65200.021.9458712
GCAGAGT108600.021.6171349
ACATGGG64950.021.2927343
GTACTTT56700.021.24366414-15
AGAGTAC90100.019.8307510-11
AGTACTT56400.019.42009212-13
CAGAGTA106500.019.07491510-11
GTGTAGC5550.018.8435881
ACTTTTT68100.018.10609416-17
Produced by FastQC (version 0.11.5)