##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936847_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1309321 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.86655678783125 33.0 31.0 34.0 30.0 34.0 2 31.95601689730784 33.0 31.0 34.0 30.0 34.0 3 31.963017472415093 33.0 31.0 34.0 30.0 34.0 4 35.53981414794386 37.0 35.0 37.0 33.0 37.0 5 35.562676379589114 37.0 35.0 37.0 33.0 37.0 6 35.63586851505475 37.0 35.0 37.0 33.0 37.0 7 35.5741999097242 37.0 35.0 37.0 33.0 37.0 8 35.546768134017555 37.0 35.0 37.0 33.0 37.0 9 37.18675939666438 39.0 37.0 39.0 34.0 39.0 10-11 37.06763047411597 39.0 37.0 39.0 33.0 39.0 12-13 36.963203828549304 39.0 37.0 39.0 33.0 39.0 14-15 38.1263968117826 40.0 38.0 41.0 33.0 41.0 16-17 38.063752509888715 40.0 38.0 41.0 33.0 41.0 18-19 38.03107832227544 40.0 38.0 41.0 33.0 41.0 20-21 37.98785973798633 40.0 38.0 41.0 33.0 41.0 22-23 37.8646783332735 40.0 38.0 41.0 33.0 41.0 24-25 37.69839863562869 40.0 37.0 41.0 32.0 41.0 26-27 37.65808537402211 40.0 37.0 41.0 32.5 41.0 28-29 37.73017312026616 40.0 37.0 41.0 33.0 41.0 30-31 37.58535416448679 40.0 37.0 41.0 32.0 41.0 32-33 37.404719316347936 40.0 37.0 41.0 32.0 41.0 34-35 37.2157939115007 40.0 36.0 41.0 31.0 41.0 36-37 37.069552844566005 39.5 36.0 41.0 31.0 41.0 38-39 36.91243514768342 39.0 36.0 41.0 31.0 41.0 40-41 36.70709054540483 39.0 36.0 41.0 30.0 41.0 42-43 36.50323602844528 39.0 35.0 41.0 30.0 41.0 44-45 36.24605081565178 39.0 35.0 41.0 30.0 41.0 46-47 35.883923041026605 39.0 35.0 40.0 28.5 41.0 48-49 35.5298643342618 39.0 35.0 40.0 27.0 41.0 50-51 34.883212367326266 38.5 34.0 39.5 26.5 40.5 52-53 35.18997633124344 38.5 35.0 40.0 27.0 40.5 54-55 35.50169362593283 39.0 35.0 40.5 27.5 41.0 56-57 35.390896502843844 39.0 35.0 41.0 27.0 41.0 58-59 35.16305321613264 38.5 35.0 40.5 26.5 41.0 60-61 34.88658128908037 38.0 34.5 40.0 26.0 41.0 62-63 34.59581034750073 38.0 34.0 40.0 26.0 41.0 64-65 34.28700601304035 37.5 34.0 40.0 25.5 41.0 66-67 33.91678549416072 37.0 34.0 40.0 24.5 41.0 68-69 33.488304243191706 36.0 33.0 39.0 23.0 41.0 70-71 33.13970179963508 36.0 33.0 39.0 22.5 41.0 72-73 32.76611083149205 35.0 33.0 38.5 22.0 40.0 74-75 32.336887592882114 35.0 32.0 37.5 20.5 40.0 76-77 31.90810274944036 35.0 32.0 37.0 20.0 39.0 78-79 31.4813281082332 35.0 31.5 36.5 18.5 39.0 80-81 31.057865870936155 35.0 31.0 36.0 17.5 38.5 82-83 30.655404977083542 35.0 31.0 36.0 13.0 37.0 84-85 30.30759378334266 34.0 31.0 35.0 8.5 37.0 86-87 29.982225138067747 34.0 30.5 35.0 7.0 36.5 88-89 29.62838524700971 34.0 30.0 35.0 2.0 36.0 90-91 29.295069352740846 34.0 30.0 35.0 2.0 36.0 92-93 28.947678987811237 34.0 29.0 35.0 2.0 35.0 94-95 28.5720255002402 34.0 29.0 35.0 2.0 35.0 96-97 28.147619262197736 34.0 29.0 35.0 2.0 35.0 98-99 27.577784592166473 34.0 28.5 35.0 2.0 35.0 100-101 25.458108057535163 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3974449391032735 1101 2 0.4051290862473529 1101 3 0.38459861817173646 1101 4 0.30367827277448356 1101 5 0.3082128521438747 1101 6 0.23546768067722468 1101 7 0.3298167871735842 1101 8 0.3013834489690481 1101 9 0.40790230990121046 1101 10-11 0.37980089627004787 1101 12-13 0.46538402149871416 1101 14-15 0.5895626593426471 1101 16-17 0.789627064094617 1101 18-19 0.6681363737826018 1101 20-21 0.7181260376066163 1101 22-23 0.6637514259293695 1101 24-25 0.7074321396688461 1101 26-27 0.688941551140033 1101 28-29 0.6968677679337034 1101 30-31 0.7557282979113111 1101 32-33 0.7360633331340409 1101 34-35 0.7503052194603299 1101 36-37 0.7690323138501256 1101 38-39 0.810343392441375 1101 40-41 0.9628535348160128 1101 42-43 0.9381676273034998 1101 44-45 1.020412528940085 1101 46-47 1.0943461853739294 1101 48-49 1.1931087375611114 1101 50-51 1.1676320312824515 1101 52-53 1.2862292282039292 1101 54-55 1.1936395990904103 1101 56-57 1.1456373504738977 1101 58-59 1.200527809468447 1101 60-61 1.218748977569696 1101 62-63 1.2860933898268812 1101 64-65 1.2735269109806282 1101 66-67 1.2403293910666946 1101 68-69 1.267955534871298 1101 70-71 1.2629539748247325 1101 72-73 1.1907567387538975 1101 74-75 1.2300792611551614 1101 76-77 1.2781792092562974 1101 78-79 1.2052187882486862 1101 80-81 1.3395359518911896 1101 82-83 1.1861209431467046 1101 84-85 1.3306466147667386 1101 86-87 1.3924887549108043 1101 88-89 1.2669590675949998 1101 90-91 1.2971403330436821 1101 92-93 0.9249064715022968 1101 94-95 1.1426135175845893 1101 96-97 1.2096889202994383 1101 98-99 1.409614469289945 1101 100-101 1.5761012438923672 1102 1 0.3239829017000986 1102 2 0.36114169491032655 1102 3 0.228829094304686 1102 4 0.17633513129857192 1102 5 0.21881229040096883 1102 6 0.19626894041975618 1102 7 0.12345949817659374 1102 8 0.1756413239401624 1102 9 0.24329084874456441 1102 10-11 0.30187768044290664 1102 12-13 0.18870281375848919 1102 14-15 0.3877843686902125 1102 16-17 0.29137522375096836 1102 18-19 0.4069513828113571 1102 20-21 0.35627612789424745 1102 22-23 0.4254974223347219 1102 24-25 0.39814185456219775 1102 26-27 0.3901093203857684 1102 28-29 0.5092104844853154 1102 30-31 0.38019248943488293 1102 32-33 0.4115458329250927 1102 34-35 0.2398139788709983 1102 36-37 0.2895805645315832 1102 38-39 0.22577520332828982 1102 40-41 0.2192590053125727 1102 42-43 0.24019574535238775 1102 44-45 0.21160616589178005 1102 46-47 0.1768303139244125 1102 48-49 0.28933354850492066 1102 50-51 0.23130254848906873 1102 52-53 0.35986755651659763 1102 54-55 0.591103081938563 1102 56-57 0.651023002641395 1102 58-59 0.531919777205097 1102 60-61 0.5273455122944384 1102 62-63 0.47300846045211387 1102 64-65 0.6395923507281154 1102 66-67 0.4827580125630959 1102 68-69 0.37086798711999336 1102 70-71 0.488739961874721 1102 72-73 0.540921932466695 1102 74-75 0.5091909656775471 1102 76-77 0.6232605875281259 1102 78-79 0.7624258907355141 1102 80-81 0.49646348228030135 1102 82-83 0.5170526932799753 1102 84-85 0.6091077636918207 1102 86-87 0.6596086390504503 1102 88-89 0.43130258061629334 1102 90-91 0.43011951596691844 1102 92-93 0.3090352326969459 1102 94-95 0.5504771105618893 1102 96-97 0.46355011263000634 1102 98-99 0.7333700907575427 1102 100-101 1.1230530968081567 1103 1 0.25411772815242273 1103 2 0.32213773587541183 1103 3 0.28159014069009203 1103 4 0.23531061470978898 1103 5 0.11708875062416979 1103 6 0.03183086877052688 1103 7 0.10570695192354407 1103 8 0.10367401753365613 1103 9 0.20060373944591703 1103 10-11 0.21592698914449926 1103 12-13 0.06703101046822013 1103 14-15 0.32797924783830723 1103 16-17 0.300722484270473 1103 18-19 0.4482098423724423 1103 20-21 0.24560490955156666 1103 22-23 0.24070014526213868 1103 24-25 0.3261942026831832 1103 26-27 0.41251962902315853 1103 28-29 0.5208706681631838 1103 30-31 0.48587091684186845 1103 32-33 0.42716919880439264 1103 34-35 0.40231707130353556 1103 36-37 0.585343697542605 1103 38-39 0.5529684502490184 1103 40-41 0.468561720378176 1103 42-43 0.6163118159759691 1103 44-45 0.6573046733738437 1103 46-47 0.4770871210626453 1103 48-49 0.6776505727878188 1103 50-51 0.5909506495010817 1103 52-53 0.5612750731291811 1103 54-55 0.5566234508810979 1103 56-57 0.6594028637109304 1103 58-59 0.6977778433871222 1103 60-61 0.5361880661857086 1103 62-63 0.5766437185827797 1103 64-65 0.7373105141802156 1103 66-67 0.7718580804343276 1103 68-69 0.7408742044420862 1103 70-71 0.708277427731737 1103 72-73 0.6422920513820074 1103 74-75 0.5239105839253142 1103 76-77 0.4678159949033649 1103 78-79 0.5090247558939183 1103 80-81 0.4820192077343215 1103 82-83 0.45009981582108693 1103 84-85 0.4904856578660741 1103 86-87 0.5831314300077786 1103 88-89 0.5041812079585206 1103 90-91 0.38939731846813075 1103 92-93 0.4405243823158642 1103 94-95 0.40297366831482506 1103 96-97 0.5585323297905376 1103 98-99 0.5106199210794671 1103 100-101 0.7277213896916308 1104 1 0.4314260790207598 1104 2 0.46711248381148707 1104 3 0.4382492619083038 1104 4 0.4006099110399788 1104 5 0.4530175964362755 1104 6 0.41143474103046174 1104 7 0.4156704315101436 1104 8 0.4261572395674733 1104 9 0.3338293439260198 1104 10-11 0.5797966914840273 1104 12-13 0.5516767425289473 1104 14-15 0.7235954696113822 1104 16-17 0.6167286889209649 1104 18-19 0.5216359686676384 1104 20-21 0.4647909145438547 1104 22-23 0.743740606691496 1104 24-25 0.571803532245454 1104 26-27 0.5980728908657227 1104 28-29 0.6805212579869604 1104 30-31 0.7353573225788566 1104 32-33 0.642817755841179 1104 34-35 0.6170102682762746 1104 36-37 0.7203085472674999 1104 38-39 0.7251649397055999 1104 40-41 0.6350310317715824 1104 42-43 0.70737743480516 1104 44-45 0.7009830213690407 1104 46-47 0.6753461320054868 1104 48-49 0.5202216829652002 1104 50-51 0.6250351230939515 1104 52-53 0.8605273928148307 1104 54-55 0.8344991220412652 1104 56-57 0.8048907907166054 1104 58-59 0.8192729881152445 1104 60-61 0.826585970953623 1104 62-63 0.9710089625753469 1104 64-65 0.9977123549817719 1104 66-67 0.9207236059280319 1104 68-69 0.9166359598134406 1104 70-71 0.7654570354237791 1104 72-73 0.8377435244052265 1104 74-75 0.8036662509003349 1104 76-77 0.7316880223260647 1104 78-79 0.8736144036272151 1104 80-81 0.8033141461943494 1104 82-83 0.7830072991012464 1104 84-85 0.8481639958966305 1104 86-87 0.7852367928120962 1104 88-89 0.6870386189119877 1104 90-91 0.7138261368774756 1104 92-93 0.6122139206041695 1104 94-95 0.6503446635666741 1104 96-97 0.5892076501953021 1104 98-99 0.8061969485935023 1104 100-101 1.1741099542683209 1105 1 0.26379299881655527 1105 2 0.31428577106682454 1105 3 0.34023262735130544 1105 4 0.34229057102483296 1105 5 0.1385390099613204 1105 6 0.23614594143226952 1105 7 0.3954722636353054 1105 8 0.32371584283441024 1105 9 0.36877390789672404 1105 10-11 0.35131188672829694 1105 12-13 0.4036997452951354 1105 14-15 0.369207346190322 1105 16-17 0.5714559316625625 1105 18-19 0.47626060415419147 1105 20-21 0.6201556341402394 1105 22-23 0.5483079882667994 1105 24-25 0.5821991933172939 1105 26-27 0.5540079943538032 1105 28-29 0.5043886425608264 1105 30-31 0.4446597244319648 1105 32-33 0.485206452738403 1105 34-35 0.43647256877778773 1105 36-37 0.3797044669658405 1105 38-39 0.31422569444521287 1105 40-41 0.6568554543597926 1105 42-43 0.6653014323463324 1105 44-45 0.6119620913631181 1105 46-47 0.5472074744644644 1105 48-49 0.6025843860244962 1105 50-51 0.41902271276988046 1105 52-53 0.4163260614505617 1105 54-55 0.7591938303180612 1105 56-57 0.6531768278838612 1105 58-59 0.7763456734080236 1105 60-61 0.6675962635389112 1105 62-63 0.7449178585775016 1105 64-65 0.7334897712654964 1105 66-67 0.7399544544478616 1105 68-69 0.8285923756248934 1105 70-71 0.602677418940651 1105 72-73 0.6746373451058432 1105 74-75 0.7037057008129963 1105 76-77 0.45885626823863745 1105 78-79 0.5887785377531713 1105 80-81 0.6617995319226644 1105 82-83 0.739353300135754 1105 84-85 0.8528670626822397 1105 86-87 0.8369471011398701 1105 88-89 0.7649629725331302 1105 90-91 0.7672404752808895 1105 92-93 0.7963517760282066 1105 94-95 0.8136574629549678 1105 96-97 0.8741058300644156 1105 98-99 1.0694881020677194 1105 100-101 1.2255532026172382 1106 1 0.4706279960702844 1106 2 0.4997523149587799 1106 3 0.4309849455316481 1106 4 0.3841160909326433 1106 5 0.4841498141356624 1106 6 0.44980085834394856 1106 7 0.4777999751793587 1106 8 0.4507432409798682 1106 9 0.5612501876076621 1106 10-11 0.6822105922090032 1106 12-13 0.4073796210126588 1106 14-15 0.7332673388669093 1106 16-17 0.7674027508283388 1106 18-19 0.8007190222990062 1106 20-21 0.8909310802755357 1106 22-23 0.8229908202700713 1106 24-25 0.7832185464884418 1106 26-27 0.7943139080022661 1106 28-29 0.7533608082420642 1106 30-31 0.6057766242628801 1106 32-33 0.6145870327950576 1106 34-35 0.5817903021843591 1106 36-37 0.7003194444032985 1106 38-39 0.8628193047755204 1106 40-41 0.9526045147024931 1106 42-43 0.9642423658649051 1106 44-45 1.1642746176142893 1106 46-47 1.1218126299252447 1106 48-49 0.9702522319745412 1106 50-51 0.9845315008542741 1106 52-53 0.9407800370894677 1106 54-55 0.9341450973745893 1106 56-57 1.070618240654582 1106 58-59 1.2703105964584367 1106 60-61 1.170180994242287 1106 62-63 1.3017105847145203 1106 64-65 1.2355198618387675 1106 66-67 1.164962935595483 1106 68-69 1.1804226880542146 1106 70-71 1.2513324166251678 1106 72-73 1.2136008142452326 1106 74-75 1.1888885974345342 1106 76-77 1.210506053382904 1106 78-79 1.3990243025642144 1106 80-81 1.3839073259627064 1106 82-83 1.4345846066094303 1106 84-85 1.413612272853154 1106 86-87 1.436671935017074 1106 88-89 1.4600495610116937 1106 90-91 1.1776923454412476 1106 92-93 1.334547230133083 1106 94-95 1.4206875266039596 1106 96-97 1.1232678119066826 1106 98-99 1.4508597324943082 1106 100-101 1.8714949061701667 1107 1 0.2972926970383867 1107 2 0.38387929908576623 1107 3 0.3987671461311919 1107 4 0.39321563718389285 1107 5 0.28825812412632246 1107 6 0.3441314560801132 1107 7 0.4512188164000577 1107 8 0.4104759059756873 1107 9 0.4687460514502746 1107 10-11 0.5494391210474348 1107 12-13 0.3647059433544584 1107 14-15 0.2993311913084753 1107 16-17 0.5033283491378384 1107 18-19 0.5020237547182873 1107 20-21 0.5170838805769407 1107 22-23 0.5937035498063352 1107 24-25 0.6004405229983405 1107 26-27 0.40195474211368065 1107 28-29 0.46729023966376104 1107 30-31 0.5451454712168626 1107 32-33 0.6209427914727925 1107 34-35 0.6289728009148376 1107 36-37 0.5671638429814436 1107 38-39 0.43462299701264584 1107 40-41 0.6954673908470994 1107 42-43 0.9060200125961089 1107 44-45 0.9410523462496059 1107 46-47 0.8950062348525947 1107 48-49 0.815831867337458 1107 50-51 0.694883360896533 1107 52-53 0.6846126578570733 1107 54-55 0.931462737881084 1107 56-57 0.846143655498885 1107 58-59 0.8186561335036586 1107 60-61 0.7948259399398623 1107 62-63 0.9001978158642885 1107 64-65 0.954797631426139 1107 66-67 0.753559426823557 1107 68-69 1.0908654616548858 1107 70-71 0.9729493674990763 1107 72-73 0.8434810704412783 1107 74-75 0.9541194851112493 1107 76-77 0.8229243540364983 1107 78-79 0.9752426261509584 1107 80-81 0.8866904275074177 1107 82-83 0.9454532815647916 1107 84-85 1.063808842709168 1107 86-87 1.001330312988145 1107 88-89 1.0824486961043291 1107 90-91 1.0148825431577642 1107 92-93 1.0011401831037432 1107 94-95 1.1405337106916313 1107 96-97 1.110547285099468 1107 98-99 1.3859884038458894 1107 100-101 1.401133545251529 1108 1 0.0012836309513879485 1108 2 0.04268957398436868 1108 3 0.02957461107300219 1108 4 0.07611466430262936 1108 5 0.06424094627726618 1108 6 0.06866158358853625 1108 7 -0.0014877551875684958 1108 8 -0.11221449510587433 1108 9 -0.1734642711645762 1108 10-11 -0.055919015898190594 1108 12-13 -0.05375162894890195 1108 14-15 0.130604552387922 1108 16-17 0.12715333654605132 1108 18-19 0.23951613337321476 1108 20-21 0.1515608574875813 1108 22-23 0.06898918456496261 1108 24-25 0.2349890742800511 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2316 52-53 -0.7708304284683649 2316 54-55 -1.319052922928698 2316 56-57 -1.0025767417509286 2316 58-59 -0.9924783202778542 2316 60-61 -0.95744678733287 2316 62-63 -0.9370061566338066 2316 64-65 -1.0016576779703499 2316 66-67 -1.0996873264232008 2316 68-69 -0.7737449279555051 2316 70-71 -1.2221423055337688 2316 72-73 -1.072752695792488 2316 74-75 -1.613565770809302 2316 76-77 -1.6132673953905474 2316 78-79 -1.7458612699529397 2316 80-81 -1.5417091141274177 2316 82-83 -1.567743815455838 2316 84-85 -1.3506487585131417 2316 86-87 -1.151247991060746 2316 88-89 -1.6835176904502944 2316 90-91 -1.6078447295695106 2316 92-93 -2.1245664631911367 2316 94-95 -2.190826105962305 2316 96-97 -2.1736582152825044 2316 98-99 -2.208243834503996 2316 100-101 -1.9960780666659659 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3258.0 3 838.0 4 885.0 5 872.0 6 962.0 7 1194.0 8 1371.0 9 1727.0 10 2341.0 11 2925.0 12 3954.0 13 4940.0 14 5628.0 15 7099.0 16 9354.0 17 12711.0 18 11219.0 19 7164.0 20 6744.0 21 6809.0 22 7448.0 23 8060.0 24 9368.0 25 10792.0 26 12585.0 27 14946.0 28 17981.0 29 22150.0 30 27150.0 31 34333.0 32 44337.0 33 59232.0 34 81841.0 35 118360.0 36 179548.0 37 268496.0 38 252950.0 39 47722.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50586471153354 19.407238812643353 14.982280141147447 22.10461633467567 2 14.899322393140435 20.658063154610893 41.17337162553772 23.269242826710954 3 18.85650692077624 25.766200134983606 30.1100582661409 25.267234678099253 4 12.422612518758863 17.213943591085084 38.163367707372444 32.20007618278361 5 13.457568018107574 37.79788031260036 34.06059308425222 14.68395858503984 6 30.440404503832845 36.97417369195771 18.146776012693312 14.438645791516125 7 27.297046376071226 33.64991503608863 21.369609074237506 17.68342951360264 8 25.651873062234067 35.1236538380668 20.192218734827602 19.03225436487153 9 26.215317079139773 17.304062528679374 20.92462296185261 35.55599743032825 10-11 24.256423883598714 27.45206820280374 27.619153219254162 20.67235469434339 12-13 25.552182524436635 24.9514210884658 28.159942076122952 21.33645431097461 14-15 22.58749193358473 25.926682019616297 26.900653876619785 24.585172170179188 16-17 21.472750840244558 29.396400426718056 27.22235604956965 21.908492683467735 18-19 21.32491657724532 27.622460092977807 30.155591226399554 20.897032103377317 20-21 22.390127635633405 26.700821066262442 30.02934618174758 20.87970511635658 22-23 21.956851932398354 26.814162742041887 29.926394652776768 21.30259067278299 24-25 21.645372876050917 26.933017713126763 30.10757660471588 21.314032806106443 26-27 21.50881015604968 27.18368098717463 29.894077892242148 21.41343096453354 28-29 21.42449297506858 27.296221189984628 30.009921506968368 21.269364327978423 30-31 21.591950757539575 26.957526505574318 29.969611297429093 21.480911439457014 32-33 21.32461672845323 27.1803548543144 29.930372622249124 21.564655794983253 34-35 21.703149853299266 27.202469172595638 29.74664464340775 21.34773633069734 36-37 21.79873691868685 26.970469513490546 29.68691968031693 21.543873887505676 38-39 21.54561339757309 27.274498857692436 29.768431960768353 21.411455783966122 40-41 21.723665750599725 27.16965888449263 29.686913007271592 21.41976235763605 42-43 21.619313133320468 27.290960057936342 29.377241641555184 21.712485167188007 44-45 21.764048880823854 27.39254254489486 29.206066810110126 21.637341764171154 46-47 21.89090859077751 27.587619145495328 28.881529005044136 21.639943258683022 48-49 21.943864000816124 27.6754121022401 28.564283670156204 21.816440226787574 50-51 21.774713258396922 27.96523765411466 28.46845126109005 21.791597826398373 52-53 22.150095994956683 28.042065849674763 28.118250503854124 21.689587651514426 54-55 21.857772119318764 27.886030619871278 28.23985412995334 22.01634313085662 56-57 21.91906130926998 28.145093867544585 28.114754850039663 21.821089973145767 58-59 22.10332993101224 27.815446181101567 28.250589164569035 21.83063472331716 60-61 21.90620562524743 27.778232086929805 28.59243475908283 21.723127528739937 62-63 21.808223010044028 27.8720900771521 28.69164725030276 21.628039662501113 64-65 21.679079116328843 28.002532284913386 28.408546041318118 21.909842557439656 66-67 21.73838934864477 28.18081896930604 28.093759626019356 21.987032056029836 68-69 21.690306718834126 28.021973612864105 28.322781000987025 21.964938667314748 70-71 21.81065651578499 27.866614970003837 28.29914796005096 22.023580554160212 72-73 21.802829146172044 27.92014249246456 28.181719909716062 22.09530845164733 74-75 21.671671098071574 28.346742153357173 27.87808288255249 22.103503866018766 76-77 21.74774382369348 28.089605159594385 27.98518297761781 22.177468039094318 78-79 21.786506861905465 28.254862148521052 27.850114629419654 22.108516360153825 80-81 21.80246307940707 28.347878446106673 27.76882478392465 22.080833690561608 82-83 21.788162731865395 28.379980281410262 27.898670905908695 21.93318608081565 84-85 21.82712910993416 28.24744844632957 27.761920466657163 22.163501977079108 86-87 21.80991715638934 28.007499760186533 27.98877329009175 22.193809793332385 88-89 21.852713068654214 28.059539231879842 27.7994497754798 22.288297923986143 90-91 21.70775488241429 28.16909320832029 27.92053463024045 22.202617279024967 92-93 21.892750218721492 28.168652327812012 27.77615761859788 22.162439834868618 94-95 21.704685277610334 28.13437094702062 27.682788056102698 22.47815571926635 96-97 21.65057859706936 28.07042560430612 27.868728997901226 22.410266800723296 98-99 21.666882724795098 28.097805117097767 27.641215019137395 22.594097138969733 100-101 21.582836701254056 28.156582024117487 27.675761868980903 22.58481940564755 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 605.0 1 428.0 2 301.5 3 365.5 4 359.5 5 377.5 6 565.5 7 1023.0 8 1660.0 9 2078.0 10 1992.5 11 1822.0 12 1888.0 13 1996.0 14 2129.5 15 2231.5 16 2317.5 17 2381.0 18 2484.0 19 2706.5 20 2925.0 21 3272.0 22 3872.0 23 4641.5 24 5593.5 25 6979.0 26 8912.0 27 11266.0 28 13282.5 29 15033.0 30 17683.5 31 20624.5 32 22924.0 33 25226.0 34 27872.0 35 30387.5 36 33761.0 37 37428.0 38 39504.5 39 40102.5 40 40774.0 41 42236.0 42 43332.5 43 44656.5 44 46777.5 45 48314.5 46 47886.0 47 47624.5 48 49657.5 49 49608.0 50 46747.5 51 44931.5 52 43327.5 53 41718.5 54 39684.5 55 36375.5 56 32995.5 57 29391.5 58 26571.5 59 24020.0 60 20280.5 61 16329.0 62 12686.5 63 9315.5 64 6813.0 65 4989.0 66 3457.0 67 2643.0 68 1922.0 69 1182.0 70 759.5 71 513.5 72 345.0 73 223.0 74 163.5 75 130.0 76 85.0 77 38.0 78 22.5 79 16.5 80 13.0 81 11.0 82 7.0 83 8.0 84 9.0 85 6.5 86 5.0 87 5.5 88 3.5 89 1.0 90 1.5 91 1.0 92 0.5 93 1.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06889066928583594 2 0.11326481435797639 3 0.0774447213479353 4 0.14809202632509522 5 0.12074960991231333 6 0.11807646864290729 7 0.1303729184821751 8 0.10967516751048825 9 0.13449719358354445 10-11 0.12246805787121723 12-13 0.10673471211414161 14-15 0.10914053925660705 16-17 0.12724152442372802 18-19 0.10497807642281763 20-21 0.08737353177715777 22-23 0.08615152433971501 24-25 0.07618452617807245 26-27 0.0656447120301286 28-29 0.08034698901186187 30-31 0.059229172983554075 32-33 0.05987836443469555 34-35 0.05380651497990179 36-37 0.05919098525113399 38-39 0.059992927631955795 40-41 0.0771774072209947 42-43 0.0754971469945109 44-45 0.060451180420996835 46-47 0.043877704550679325 48-49 0.05311913579634024 50-51 0.03310876400821494 52-53 0.050293243597253845 54-55 0.05796897781369122 56-57 0.05919098525113399 58-59 0.06877610608857568 60-61 0.0659120261570692 62-63 0.07114374549862104 64-65 0.04746735139816745 66-67 0.077750223207296 68-69 0.06434632912784566 70-71 0.03081750006300976 72-73 0.02592947031323869 74-75 0.03673659858812316 76-77 0.05277544620455947 78-79 0.07442789048674847 80-81 0.048422044708669607 82-83 0.0695780484693975 84-85 0.07267125479542451 86-87 0.07733015815067504 88-89 0.1000136712082064 90-91 0.074657016881269 92-93 0.08786997229861891 94-95 0.09588939610683705 96-97 0.10814765821368481 98-99 0.13766677537441163 100-101 0.13468813224564488 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1309321.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.202353398124814 #Duplication Level Percentage of deduplicated Percentage of total 1 81.33858662045911 50.59451509869785 2 11.06163966524991 13.761200392411798 3 3.2021814332856087 5.975496634744356 4 1.4095585063905636 3.507114253993552 5 0.7644908129627228 2.3776563858763513 6 0.4630357870032927 1.7281149395494595 7 0.31854568589390475 1.3870003929194508 8 0.22611017221735263 1.1251667871339706 9 0.1641028157809884 0.9186823206752773 >10 0.9465038789919676 10.814307518510592 >50 0.07421310332580836 3.115858300417367 >100 0.030046399682157842 3.23450990235619 >500 3.6941953370370694E-4 0.1537279337531006 >1k 4.925593782716093E-4 0.7941460972357484 >5k 1.2313984456790232E-4 0.5125030417249606 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6691 0.5110282352455968 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4913 0.3752326587597694 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3049 0.2328687922976871 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.637546484017288E-5 0.0 5 0.0 0.0 0.0 7.637546484017288E-5 0.0 6 1.5275092968034575E-4 0.0 0.0 7.637546484017288E-5 0.0 7 1.5275092968034575E-4 0.0 0.0 7.637546484017288E-5 0.0 8 1.5275092968034575E-4 0.0 0.0 7.637546484017288E-5 0.0 9 1.5275092968034575E-4 0.0 0.0 1.5275092968034575E-4 0.0 10-11 1.5275092968034575E-4 0.0 0.0 1.9093866210043219E-4 0.0 12-13 1.5275092968034575E-4 0.0 0.0 2.2912639452051865E-4 3.818773242008644E-5 14-15 1.5275092968034575E-4 0.0 0.0 4.2006505662095083E-4 7.637546484017288E-5 16-17 1.5275092968034575E-4 0.0 0.0 7.637546484017289E-4 7.637546484017288E-5 18-19 1.5275092968034575E-4 0.0 0.0 9.165055780820746E-4 7.637546484017288E-5 20-21 1.5275092968034575E-4 0.0 0.0 9.54693310502161E-4 7.637546484017288E-5 22-23 1.5275092968034575E-4 0.0 0.0 0.001031068775342334 7.637546484017288E-5 24-25 2.2912639452051865E-4 0.0 0.0 0.001298382902282939 7.637546484017288E-5 26-27 2.2912639452051865E-4 0.0 0.0 0.0014511338319632848 7.637546484017288E-5 28-29 2.2912639452051865E-4 0.0 0.0 0.0022912639452051867 7.637546484017288E-5 30-31 2.2912639452051865E-4 0.0 0.0 0.004315213763469768 7.637546484017288E-5 32-33 2.2912639452051865E-4 0.0 0.0 0.006148224919633917 7.637546484017288E-5 34-35 2.2912639452051865E-4 0.0 0.0 0.008248550202738672 1.5275092968034575E-4 36-37 2.2912639452051865E-4 0.0 0.0 0.011189005599085328 1.5275092968034575E-4 38-39 3.055018593606915E-4 0.0 0.0 0.013671208206390947 1.5275092968034575E-4 40-41 3.055018593606915E-4 0.0 0.0 0.017719107842920107 1.5275092968034575E-4 42-43 3.055018593606915E-4 0.0 0.0 0.02325632904383264 1.5275092968034575E-4 44-45 3.055018593606915E-4 0.0 0.0 0.03345245359999573 1.5275092968034575E-4 46-47 3.055018593606915E-4 0.0 0.0 0.04613078076346442 1.5275092968034575E-4 48-49 3.055018593606915E-4 0.0 0.0 0.07221300200638346 2.2912639452051865E-4 50-51 3.055018593606915E-4 0.0 0.0 0.09676771395249904 2.2912639452051865E-4 52-53 3.055018593606915E-4 0.0 0.0 0.12716514895888784 2.2912639452051865E-4 54-55 3.055018593606915E-4 0.0 0.0 0.18016972155796784 2.2912639452051865E-4 56-57 3.055018593606915E-4 0.0 0.0 0.23787138524471846 2.2912639452051865E-4 58-59 3.055018593606915E-4 0.0 0.0 0.2993918221734777 2.2912639452051865E-4 60-61 3.055018593606915E-4 0.0 0.0 0.3685879933186743 2.2912639452051865E-4 62-63 3.055018593606915E-4 0.0 0.0 0.45634340242003296 2.2912639452051865E-4 64-65 3.055018593606915E-4 0.0 0.0 0.520422417420938 2.2912639452051865E-4 66-67 3.818773242008644E-4 0.0 0.0 0.6207415904885051 2.2912639452051865E-4 68-69 3.818773242008644E-4 0.0 0.0 0.7612342580620031 2.2912639452051865E-4 70-71 3.818773242008644E-4 0.0 0.0 0.8681217211058251 2.2912639452051865E-4 72-73 4.2006505662095083E-4 0.0 0.0 1.010714713962428 3.055018593606915E-4 74-75 4.582527890410373E-4 0.0 0.0 1.1845070842062413 3.055018593606915E-4 76-77 4.582527890410373E-4 0.0 0.0 1.3165602629149 3.055018593606915E-4 78-79 4.582527890410373E-4 0.0 0.0 1.4437254118737881 3.055018593606915E-4 80-81 5.346282538812102E-4 0.0 0.0 1.6466168342217071 3.055018593606915E-4 82-83 5.728159863012967E-4 0.0 0.0 1.8688312491741903 3.055018593606915E-4 84-85 6.11003718721383E-4 0.0 0.0 2.1306845303787227 3.055018593606915E-4 86-87 6.11003718721383E-4 0.0 0.0 2.4696006556069903 3.055018593606915E-4 88-89 6.11003718721383E-4 0.0 0.0 2.8111517343722436 3.055018593606915E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4580 0.0 41.932407 1 GTATCAA 7100 0.0 34.882957 1 ATCAACG 8830 0.0 28.041075 3 TATCAAC 8825 0.0 27.956743 2 TCAACGC 9010 0.0 27.480873 4 CAACGCA 9030 0.0 27.414764 5 AACGCAG 9300 0.0 26.567759 6 GAGTACT 5185 0.0 23.323757 12-13 ACGCAGA 10500 0.0 23.30518 7 CGCAGAG 10480 0.0 23.170073 8 GTACATG 6455 0.0 22.903301 1 TACATGG 6520 0.0 21.945871 2 GCAGAGT 10860 0.0 21.617134 9 ACATGGG 6495 0.0 21.292734 3 GTACTTT 5670 0.0 21.243664 14-15 AGAGTAC 9010 0.0 19.83075 10-11 AGTACTT 5640 0.0 19.420092 12-13 CAGAGTA 10650 0.0 19.074915 10-11 GTGTAGC 555 0.0 18.843588 1 ACTTTTT 6810 0.0 18.106094 16-17 >>END_MODULE