FastQCFastQC Report
Thu 26 May 2016
SRR936847_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936847_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309321
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81910.6255914325058561No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67320.5141596293040438No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45340.3462863575853438No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17620.1345735690483846No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48800.039.819111
GTATCAA82350.038.4813461
TATCAAC101500.030.98232
ATCAACG102450.030.5535743
TCAACGC103850.030.1405284
CAACGCA104400.030.027245
AACGCAG107650.029.1637126
ACGCAGA120000.026.043547
CGCAGAG120150.025.8133748
GAGTACT70200.024.18768512-13
GCAGAGT125500.024.1452859
GTACATG61600.023.2924041
ACATGGG59500.022.9114973
TACATGG62000.022.9087412
GTACTTT75450.022.2851714-15
AGTACTT72450.022.22371512-13
AGAGTAC102150.022.03917510-11
CATGGGG25800.020.0668284
CAGAGTA120750.019.82434310-11
CATGGGA37650.018.7972034
Produced by FastQC (version 0.11.5)