##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936847_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1309321 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.091962933459406 31.0 31.0 34.0 31.0 34.0 2 32.09678146153617 33.0 31.0 34.0 30.0 34.0 3 32.2673187094685 33.0 31.0 34.0 30.0 34.0 4 36.019619329408144 37.0 35.0 37.0 35.0 37.0 5 35.94548395695173 37.0 35.0 37.0 35.0 37.0 6 35.99241515258672 37.0 35.0 37.0 35.0 37.0 7 35.933050031275755 37.0 35.0 37.0 35.0 37.0 8 35.91488030819028 37.0 35.0 37.0 35.0 37.0 9 37.59875767668891 39.0 37.0 39.0 35.0 39.0 10-11 37.51967775663874 39.0 37.0 39.0 35.0 39.0 12-13 37.415736859028456 39.0 37.0 39.0 34.0 39.0 14-15 38.600800720373385 40.0 38.0 41.0 34.0 41.0 16-17 38.520887162124495 40.0 38.0 41.0 34.0 41.0 18-19 38.594571155583694 40.0 38.0 41.0 34.0 41.0 20-21 38.68507417203268 40.0 38.0 41.0 34.0 41.0 22-23 38.613785695028184 40.0 38.0 41.0 34.0 41.0 24-25 38.510488260709174 40.0 38.0 41.0 34.0 41.0 26-27 38.35317466075928 40.0 38.0 41.0 34.0 41.0 28-29 38.170144678043044 40.0 38.0 41.0 34.0 41.0 30-31 38.007073895553496 40.0 37.0 41.0 33.0 41.0 32-33 37.974534128758336 40.0 37.0 41.0 33.0 41.0 34-35 37.873193433848535 40.0 37.0 41.0 33.0 41.0 36-37 37.69613639436013 40.0 37.0 41.0 33.0 41.0 38-39 37.54305475891702 40.0 37.0 41.0 33.0 41.0 40-41 37.3514203163319 40.0 36.0 41.0 32.5 41.0 42-43 37.105036885530744 40.0 36.0 41.0 31.5 41.0 44-45 36.71052896883194 39.0 36.0 41.0 31.0 41.0 46-47 36.38930292876995 39.0 36.0 41.0 30.0 41.0 48-49 36.014224166571836 39.0 35.0 41.0 29.5 41.0 50-51 35.73955851926304 39.0 35.0 40.0 28.5 41.0 52-53 35.4870008958842 39.0 35.0 40.0 27.5 41.0 54-55 35.252939882580364 38.5 35.0 40.0 27.0 41.0 56-57 34.97780949056801 38.0 34.5 40.0 26.5 41.0 58-59 34.656457430989036 38.0 34.0 40.0 26.0 41.0 60-61 34.33617653730445 38.0 34.0 40.0 26.0 41.0 62-63 33.96293460503574 37.0 33.5 40.0 25.0 41.0 64-65 33.574984285748116 36.5 33.0 39.0 23.5 41.0 66-67 33.192666656992444 36.0 32.5 39.0 22.5 40.0 68-69 32.757030170599876 35.5 32.0 39.0 22.0 40.0 70-71 32.28386965457669 35.0 31.5 38.0 20.5 40.0 72-73 31.942975022931734 35.0 31.0 37.5 20.0 39.5 74-75 31.50106543773452 35.0 31.0 37.0 18.5 39.0 76-77 30.12367822711161 33.5 29.5 35.5 18.0 38.0 78-79 30.845810538439387 34.0 31.0 36.0 18.0 38.5 80-81 30.667552112889048 34.0 31.0 36.0 16.0 37.5 82-83 30.322179969617842 34.0 30.5 35.0 11.5 37.0 84-85 29.9388324177188 34.0 30.0 35.0 7.5 36.5 86-87 29.57047813332254 34.0 30.0 35.0 4.5 36.0 88-89 29.207678636484104 34.0 29.0 35.0 2.0 36.0 90-91 28.77470498067319 34.0 29.0 35.0 2.0 35.0 92-93 28.30465638296491 34.0 29.0 35.0 2.0 35.0 94-95 27.80928970053944 33.0 27.5 35.0 2.0 35.0 96-97 27.156304298182036 33.0 26.5 35.0 2.0 35.0 98-99 26.41572502083141 33.0 25.0 34.5 2.0 35.0 100-101 23.4039975682052 29.5 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39542005749547116 1101 2 0.22190704414494888 1101 3 0.2632590142866462 1101 4 0.2511446946203719 1101 5 0.26236966351747526 1101 6 0.18780881600745403 1101 7 0.29772241983249614 1101 8 0.3268399189697959 1101 9 0.42204596722886834 1101 10-11 0.3771936625168024 1101 12-13 0.36881891231815445 1101 14-15 0.5789803821048665 1101 16-17 0.5697637942225668 1101 18-19 0.5344180437481967 1101 20-21 0.465458133744832 1101 22-23 0.44837912172312855 1101 24-25 0.5123707948268859 1101 26-27 0.4593502719736833 1101 28-29 0.47550375695713143 1101 30-31 0.5592532284115919 1101 32-33 0.668333853526569 1101 34-35 0.6595362565782921 1101 36-37 0.6386462777484141 1101 38-39 0.45588595293720147 1101 40-41 0.52230032983271 1101 42-43 0.5240017880624279 1101 44-45 0.6758716772268798 1101 46-47 0.6596991667962868 1101 48-49 0.5126654912623678 1101 50-51 0.5364240502190754 1101 52-53 0.35490652248566334 1101 54-55 0.44176172441810735 1101 56-57 0.5188295801665035 1101 58-59 0.6282531926253228 1101 60-61 0.5900931352374243 1101 62-63 0.6992610238130865 1101 64-65 0.7769646483681143 1101 66-67 0.6138629036299292 1101 68-69 0.49307916592787393 1101 70-71 0.5222987008106159 1101 72-73 0.611155213604242 1101 74-75 0.6992273167616823 1101 76-77 0.4639233177639497 1101 78-79 0.5347021871408622 1101 80-81 0.6411293016963491 1101 82-83 0.8207768367981849 1101 84-85 0.8218439086067626 1101 86-87 0.6150348578124074 1101 88-89 0.6768530590530482 1101 90-91 0.7822505027267255 1101 92-93 1.0314891813863056 1101 94-95 0.9922818129498552 1101 96-97 0.9667997939323563 1101 98-99 1.0704038509236575 1101 100-101 1.2622296707826486 1102 1 0.26924071696394236 1102 2 0.06998677258670938 1102 3 0.18260276259660913 1102 4 0.15859231831021958 1102 5 0.2755089551089611 1102 6 0.22901102168064824 1102 7 0.23684867713434699 1102 8 0.20170181857106684 1102 9 0.3218024610204111 1102 10-11 0.2986379767135574 1102 12-13 0.2823505038598384 1102 14-15 0.39810257277576966 1102 16-17 0.39596079896275427 1102 18-19 0.33314808196328727 1102 20-21 0.31540278823220547 1102 22-23 0.378756679258224 1102 24-25 0.4078822597060068 1102 26-27 0.41507280834333926 1102 28-29 0.3494852555422412 1102 30-31 0.22480163009920773 1102 32-33 0.48810765332611084 1102 34-35 0.4933339631108211 1102 36-37 0.40285876658976605 1102 38-39 0.46347436353067195 1102 40-41 0.4847171798723551 1102 42-43 0.5073684598094559 1102 44-45 0.49322446202624803 1102 46-47 0.5995597354363085 1102 48-49 0.5738456359894712 1102 50-51 0.627024057383089 1102 52-53 0.6175058792696646 1102 54-55 0.5943515151376246 1102 56-57 0.5809615904517855 1102 58-59 0.7280903869168682 1102 60-61 0.5922670971403221 1102 62-63 0.7538599338997969 1102 64-65 0.6429084052467928 1102 66-67 0.5919347024925301 1102 68-69 0.577579753447182 1102 70-71 0.6729480624057445 1102 72-73 0.6088838000531638 1102 74-75 0.316096952092348 1102 76-77 0.3607144720339477 1102 78-79 0.5209469308974626 1102 80-81 0.6182104299300768 1102 82-83 0.5947896252638394 1102 84-85 0.5994810204419245 1102 86-87 0.6667451605762444 1102 88-89 0.7231238039629098 1102 90-91 0.8528757314308173 1102 92-93 0.7552197288082105 1102 94-95 1.173830370560104 1102 96-97 1.3839219958409323 1102 98-99 1.382921102499214 1102 100-101 1.4185363200402996 1103 1 0.06930909308163535 1103 2 -0.15174550894690597 1103 3 0.04347034589754628 1103 4 0.0967512408526261 1103 5 0.18499787247988309 1103 6 0.12607827767059376 1103 7 0.1373578195550209 1103 8 0.26753474859589943 1103 9 0.15292859692587513 1103 10-11 0.19691587947088607 1103 12-13 0.3291172799908111 1103 14-15 0.3340787608649123 1103 16-17 0.35179761661706976 1103 18-19 0.3873918731160799 1103 20-21 0.3085565212580903 1103 22-23 0.4102282660241272 1103 24-25 0.3234076414546365 1103 26-27 0.355040848999721 1103 28-29 0.39111444637435255 1103 30-31 0.5053761409539135 1103 32-33 0.5428307264431211 1103 34-35 0.46315001837141523 1103 36-37 0.549722805584345 1103 38-39 0.5311416560167288 1103 40-41 0.5859722566909014 1103 42-43 0.6099863707459505 1103 44-45 0.7426404160209543 1103 46-47 0.8519458672017066 1103 48-49 0.7043745988290766 1103 50-51 0.5849835640075653 1103 52-53 0.7996894648180728 1103 54-55 0.8944907557443855 1103 56-57 0.7897413580451129 1103 58-59 0.6637120722439178 1103 60-61 0.6640052209371561 1103 62-63 0.7291105561729623 1103 64-65 0.7216181161701698 1103 66-67 0.657776287722136 1103 68-69 0.7924578340689692 1103 70-71 0.8295625502087631 1103 72-73 0.9275599648453721 1103 74-75 0.9692324082108179 1103 76-77 0.9920718100968671 1103 78-79 0.9055898207549298 1103 80-81 0.9083716619696247 1103 82-83 0.8547268822419198 1103 84-85 0.8615718005571686 1103 86-87 0.622198264152356 1103 88-89 0.8918745117062166 1103 90-91 1.1755718782057798 1103 92-93 1.1564485154422641 1103 94-95 1.0513758121883257 1103 96-97 1.1755809389394507 1103 98-99 1.2869844686958807 1103 100-101 1.5585607637811059 1104 1 0.23123286689089895 1104 2 0.1258195975335994 1104 3 0.1275818901015242 1104 4 0.17878598755972774 1104 5 0.14415695633068992 1104 6 0.1322239707031514 1104 7 0.1587793089700753 1104 8 0.307712185656726 1104 9 0.26886803847411755 1104 10-11 0.3039070723880215 1104 12-13 0.32419723794001953 1104 14-15 0.34130727834206454 1104 16-17 0.3129799380133136 1104 18-19 0.31770539397291486 1104 20-21 0.22861102785053333 1104 22-23 0.29019709330200527 1104 24-25 0.36067747898115243 1104 26-27 0.3382807361335409 1104 28-29 0.3110780256073795 1104 30-31 0.4163956234900539 1104 32-33 0.30921965639002735 1104 34-35 0.29962193717950925 1104 36-37 0.17652412224572345 1104 38-39 0.09196334040661469 1104 40-41 0.3916438818742094 1104 42-43 0.25944271647917816 1104 44-45 0.5077210714951121 1104 46-47 0.5522418170729111 1104 48-49 0.5730692639257597 1104 50-51 0.5405968960344225 1104 52-53 0.4599109455971018 1104 54-55 0.5296623717934423 1104 56-57 0.7237550826788706 1104 58-59 0.7342683816061921 1104 60-61 0.5274244384138171 1104 62-63 0.5597199438623122 1104 64-65 0.6440703647747057 1104 66-67 0.7056032483900267 1104 68-69 0.529925220625671 1104 70-71 0.6428442556240412 1104 72-73 0.43108080506399915 1104 74-75 0.46767460249144577 1104 76-77 0.3027494223693239 1104 78-79 0.3533804935177436 1104 80-81 0.5625475113308092 1104 82-83 0.47010415189675925 1104 84-85 0.5120304232111188 1104 86-87 0.46010426104006896 1104 88-89 0.68173141944526 1104 90-91 0.9425159217579093 1104 92-93 1.1114423788834884 1104 94-95 0.9854999782723333 1104 96-97 0.9503675710398802 1104 98-99 1.1203316418099476 1104 100-101 0.8148974457877856 1105 1 0.2030697522781324 1105 2 0.028755008374922397 1105 3 0.09165904326089702 1105 4 0.11804342963113612 1105 5 0.16556111084690883 1105 6 0.1258492021551305 1105 7 0.21076511074972615 1105 8 0.21342220912052312 1105 9 0.2316942264693509 1105 10-11 0.35715039869970866 1105 12-13 0.3166047695936598 1105 14-15 0.4088879773083107 1105 16-17 0.38708158572653417 1105 18-19 0.33889168376953194 1105 20-21 0.24930161684429208 1105 22-23 0.36855342815211856 1105 24-25 0.2094273571643157 1105 26-27 0.2741015379506706 1105 28-29 0.31116751412661614 1105 30-31 0.42554078792403516 1105 32-33 0.33951690820228464 1105 34-35 0.4144038475673 1105 36-37 0.3521975819187304 1105 38-39 0.44820448525995715 1105 40-41 0.22016232681674808 1105 42-43 0.3074291779438241 1105 44-45 0.19117534707852002 1105 46-47 0.09145699807397989 1105 48-49 0.23234336679163903 1105 50-51 0.0591927040361 1105 52-53 0.23193636595888023 1105 54-55 0.25176022631790573 1105 56-57 0.5289071850499809 1105 58-59 0.36226179600082276 1105 60-61 0.4757605141876269 1105 62-63 0.33658289805168096 1105 64-65 0.3121490134699556 1105 66-67 0.2947947219614022 1105 68-69 0.15713760171369984 1105 70-71 0.16051285012017047 1105 72-73 0.4382872077567157 1105 74-75 0.3493295497541311 1105 76-77 0.3108244727827518 1105 78-79 0.4122761208556902 1105 80-81 0.5998187768015448 1105 82-83 0.6489270452151992 1105 84-85 0.6668736730593174 1105 86-87 0.48810667113924566 1105 88-89 0.6432700972266154 1105 90-91 0.7161254294679757 1105 92-93 0.8387816368877914 1105 94-95 0.6351388382167116 1105 96-97 0.7153107481728007 1105 98-99 0.7604987745040823 1105 100-101 0.549320821126063 1106 1 0.3624547479163951 1106 2 0.28655631797181513 1106 3 0.30986954803726263 1106 4 0.28495106858733976 1106 5 0.17178331152970827 1106 6 0.24598398509159836 1106 7 0.25547287463457735 1106 8 0.34203948589133404 1106 9 0.39464399653601134 1106 10-11 0.4164861229089425 1106 12-13 0.4513803018866369 1106 14-15 0.5679855562772218 1106 16-17 0.5220455879666304 1106 18-19 0.5612438156338726 1106 20-21 0.5431659777157734 1106 22-23 0.4960775792296275 1106 24-25 0.5799922554443953 1106 26-27 0.5485333010601039 1106 28-29 0.5996914444250407 1106 30-31 0.46083649727107456 1106 32-33 0.7014054578452686 1106 34-35 0.6907781973459777 1106 36-37 0.4743227421181828 1106 38-39 0.6394987891596671 1106 40-41 0.9228415617364689 1106 42-43 0.9701568227156727 1106 44-45 1.0463146532937202 1106 46-47 1.0378110469469277 1106 48-49 1.1602542851249211 1106 50-51 0.9203455477783606 1106 52-53 1.0723332836675183 1106 54-55 1.1372034807092533 1106 56-57 1.1783355826246478 1106 58-59 0.9609830274096112 1106 60-61 1.0820205434812493 1106 62-63 0.9851625077573445 1106 64-65 1.0974851092342277 1106 66-67 1.2937298667601667 1106 68-69 1.2737466504251103 1106 70-71 1.2783476265948153 1106 72-73 1.4190033666314932 1106 74-75 1.3977649799502423 1106 76-77 1.264262299239622 1106 78-79 1.175048998414841 1106 80-81 1.1896287452462921 1106 82-83 1.3548742143965953 1106 84-85 1.2936856769794893 1106 86-87 1.0639907298126339 1106 88-89 1.2286644840531054 1106 90-91 1.1608613292235042 1106 92-93 1.221761992934443 1106 94-95 1.3463234526072014 1106 96-97 1.6332172778487397 1106 98-99 1.7940450788776658 1106 100-101 1.8486124433676672 1107 1 0.16802750333435057 1107 2 0.18089182995438335 1107 3 0.08729022390994601 1107 4 0.1519830275697629 1107 5 0.07169649317663129 1107 6 0.16016486212077297 1107 7 0.22168251638601788 1107 8 0.17639170631166934 1107 9 0.21776716393162587 1107 10-11 0.2752826239655022 1107 12-13 0.27806711696354114 1107 14-15 0.22583688394279022 1107 16-17 0.3152090521827233 1107 18-19 0.3863375958360109 1107 20-21 0.38596824482144854 1107 22-23 0.31099968842418235 1107 24-25 0.4242556918130944 1107 26-27 0.47139191819117343 1107 28-29 0.4085182500010731 1107 30-31 0.32424256145112906 1107 32-33 0.40799791939129193 1107 34-35 0.45883313862074004 1107 36-37 0.5361087430682758 1107 38-39 0.4999710482387272 1107 40-41 0.4065532920426236 1107 42-43 0.3986660041660244 1107 44-45 0.3007161799862672 1107 46-47 0.37310598363505676 1107 48-49 0.3909945344410204 1107 50-51 0.35298187716391993 1107 52-53 0.47091798070422897 1107 54-55 0.5041854781866064 1107 56-57 0.4088006341423309 1107 58-59 0.4998465260254292 1107 60-61 0.39927773875702144 1107 62-63 0.33321400905962406 1107 64-65 0.4742358095143331 1107 66-67 0.46611999240106883 1107 68-69 0.6306298715498144 1107 70-71 0.5963960155410319 1107 72-73 0.671594812566596 1107 74-75 0.8657265505433962 1107 76-77 0.7736517860284913 1107 78-79 0.6557646764989435 1107 80-81 0.6115823219986289 1107 82-83 0.7783684073954333 1107 84-85 0.6956923275929547 1107 86-87 0.7547224611016397 1107 88-89 0.6979070348746035 1107 90-91 1.0545063895868338 1107 92-93 1.2979057837841204 1107 94-95 1.048986974156172 1107 96-97 1.0309788386133967 1107 98-99 1.1229564094917812 1107 100-101 1.1544382824752404 1108 1 -0.11015026619617174 1108 2 -0.2076310870988749 1108 3 -0.14363122984505594 1108 4 0.05179083712891952 1108 5 -0.023199193804785523 1108 6 -0.023973461978584965 1108 7 0.02473237986453114 1108 8 0.019324399893832833 1108 9 0.033594378098051436 1108 10-11 -0.0528374884327647 1108 12-13 0.0415370159641526 1108 14-15 -0.16459203210312978 1108 16-17 0.007581742583809614 1108 18-19 -0.04496094881844215 1108 20-21 0.05264507328153911 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-1.092741558636412 2316 98-99 -1.1537815584226543 2316 100-101 -1.3239230620625726 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 4.0 4 8.0 5 24.0 6 54.0 7 159.0 8 354.0 9 775.0 10 1249.0 11 2060.0 12 3239.0 13 4411.0 14 5759.0 15 8116.0 16 11004.0 17 15335.0 18 13494.0 19 7809.0 20 6765.0 21 6864.0 22 7022.0 23 7780.0 24 8914.0 25 10612.0 26 12893.0 27 16129.0 28 20408.0 29 25053.0 30 31562.0 31 39728.0 32 50341.0 33 65470.0 34 90190.0 35 127639.0 36 192295.0 37 286790.0 38 212852.0 39 16159.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.499467185908955 19.907844169256894 15.70269966778373 21.889988977050418 2 15.375532260220352 21.117412308338583 40.42237115961124 23.08468427182983 3 19.13713341689026 25.531529756271926 30.774917951526287 24.556418875311525 4 12.931827208016374 17.78892860416094 37.650154278556805 31.62908990926588 5 13.385162832154455 37.715774849479835 34.156831205849265 14.74223111251645 6 30.55162432264199 37.22114235306248 17.65144369383991 14.57578963045562 7 27.232512118876883 33.77468168615641 21.206640693916924 17.78616550104978 8 26.07458369643502 34.84042492253619 19.908105040704307 19.17688634032449 9 26.206178622354642 16.97246129864258 20.810022905001905 36.01133717400087 10-11 24.407191830256796 27.288116952400447 27.544547967302087 20.76014325004067 12-13 25.736664953299936 24.784027959701003 28.283916832720674 21.19539025427839 14-15 22.657744990626817 25.796815982030374 27.195251277284665 24.35018775005814 16-17 21.300620703402757 29.090154362451987 27.690230279664043 21.918994654481217 18-19 21.084860015229268 26.991585715038557 31.054760444535756 20.86879382519642 20-21 22.034783764317886 26.117075756383805 31.156070202988033 20.69207027631027 22-23 21.64660155912874 26.19838832494094 31.083172117456296 21.07183799847402 24-25 21.498806966946415 26.223737233324268 31.314100703599262 20.96335509613005 26-27 21.42180934570588 26.42079162359328 31.092575287568554 21.064823743132287 28-29 21.09232179866179 26.654634649688436 31.174546097505928 21.078497454143847 30-31 21.496762799175144 26.263169091845906 31.08793704884772 21.15213106013123 32-33 21.226742574159122 26.329265805808085 31.116814695951234 21.32717692408156 34-35 21.50082162882999 26.38545562306885 31.024651156218695 21.08907159188247 36-37 21.57343259562569 26.127002421599705 30.955651615017203 21.3439133677574 38-39 21.405900991263575 26.506266571500475 30.991221568050126 21.096610869185824 40-41 21.51344700231694 26.498937002656824 30.839716345641598 21.147899649384637 42-43 21.45305581884519 26.542230172253795 30.56429863842131 21.440415370479705 44-45 21.650710486017495 26.59654093934615 30.247648579600785 21.50509999503557 46-47 21.806327638135688 27.02598563604987 29.762636239664957 21.405050486149484 48-49 21.874585900985892 27.139347683546823 29.40976546195597 21.576300953511314 50-51 21.738086082121285 27.354629637762212 29.248055955784665 21.65922832433184 52-53 22.139994951545145 27.470659197601183 28.82725004706672 21.562095803786953 54-55 21.826189701499604 27.312839595563354 28.987770358930646 21.873200344006392 56-57 21.861746449872033 27.521711668432214 28.853701809872156 21.762840071823597 58-59 22.069435364132396 27.1277335133781 29.052114711327544 21.75071641116196 60-61 21.899944016185664 27.133896022857897 29.434807879282705 21.53135208167374 62-63 21.730690945917768 27.23579626386501 29.504414883745085 21.529097906472135 64-65 21.64425359013379 27.29975731631174 29.190857068663632 21.86513202489083 66-67 21.702020246379703 27.582131342173287 28.883226070316624 21.83262234113038 68-69 21.75818611326023 27.293230613424825 29.107071527914087 21.84151174540086 70-71 21.788158577092368 27.263142137248447 29.08702921136866 21.86167007429053 72-73 21.845988052581472 27.242985961038567 28.91560927977527 21.995416706604683 74-75 21.759504474659508 27.565239141766607 28.620139882037783 22.055116501536105 76-77 21.748798609350224 27.53464404314925 28.63556238524543 22.0809949622551 78-79 21.75164274266136 27.572147210822113 28.586338170324353 22.089871876192174 80-81 21.769154330695432 27.702068440867155 28.51868287649313 22.010094351944286 82-83 21.833875863248856 27.66230453265796 28.57626758766912 21.927552016424066 84-85 21.77344423979289 27.6907425236095 28.3981970126135 22.137616223984104 86-87 21.68887127133916 27.46772035907723 28.561166821520345 22.282241548063258 88-89 21.697389072828717 27.395144752368623 28.432488992251614 22.474977182551047 90-91 21.57594902388188 27.459007316016955 28.535482949533307 22.429560710567863 92-93 21.710604198664804 27.28089597585313 28.532346154991785 22.476153670490277 94-95 21.551928903498688 27.33661544665586 28.468807066879805 22.64264858296565 96-97 21.6217093517707 27.182668568449873 28.555771320116325 22.6398507596631 98-99 21.470668993121073 27.206112327420577 28.34661726578978 22.97660141366857 100-101 21.171478739581353 27.369509612307542 28.373701893591363 23.08530975451974 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 653.0 1 473.0 2 341.0 3 398.5 4 327.0 5 308.0 6 588.5 7 1335.5 8 2246.0 9 2707.0 10 2440.0 11 2024.0 12 2029.0 13 2210.5 14 2410.5 15 2528.5 16 2621.5 17 2748.5 18 2885.0 19 3056.0 20 3248.0 21 3653.0 22 4316.0 23 5055.5 24 5963.0 25 7274.0 26 9076.5 27 11253.5 28 13108.5 29 14975.5 30 17736.0 31 20711.5 32 23065.0 33 24915.0 34 27382.0 35 30122.5 36 33395.5 37 37016.0 38 39035.5 39 39578.0 40 40480.0 41 41837.0 42 42747.5 43 43809.5 44 45752.5 45 47603.5 46 47482.0 47 47667.0 48 49933.0 49 49457.0 50 46540.5 51 44868.5 52 43243.0 53 41772.5 54 39639.0 55 36045.0 56 32422.5 57 29110.0 58 26703.0 59 24188.0 60 20579.5 61 16667.0 62 12849.0 63 9597.5 64 6908.0 65 4872.5 66 3503.5 67 2731.5 68 1968.0 69 1170.5 70 758.0 71 512.0 72 340.5 73 229.0 74 161.0 75 118.5 76 80.5 77 42.0 78 15.5 79 9.5 80 6.5 81 3.5 82 5.0 83 4.0 84 0.5 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017871858772600453 2 0.003513271382647953 3 0.002596765804565878 4 7.637546484017288E-5 5 0.002138513015524841 6 4.582527890410373E-4 7 0.0 8 0.0 9 0.0 10-11 1.5275092968034575E-4 12-13 6.11003718721383E-4 14-15 3.818773242008644E-5 16-17 0.0 18-19 0.0 20-21 0.002444014874885532 22-23 0.0 24-25 0.004047899636529163 26-27 0.0018711988885842358 28-29 0.0027877044666663104 30-31 0.018521050223741925 32-33 6.11003718721383E-4 34-35 0.002405827142465446 36-37 0.004773466552510805 38-39 0.0024822026073056187 40-41 0.003933336439268903 42-43 0.002444014874885532 44-45 4.582527890410373E-4 46-47 1.1456319726025932E-4 48-49 1.9093866210043219E-4 50-51 1.1456319726025932E-4 52-53 7.255669159816424E-4 54-55 0.0032841449881274337 56-57 1.5275092968034575E-4 58-59 0.001298382902282939 60-61 0.0010692565077624204 62-63 0.0 64-65 2.673141269406051E-4 66-67 3.818773242008644E-5 68-69 0.0 70-71 1.5275092968034575E-4 72-73 3.818773242008644E-5 74-75 2.673141269406051E-4 76-77 3.8187732420086437E-4 78-79 1.1456319726025932E-4 80-81 0.005651784398172793 82-83 0.0017566356913239765 84-85 0.003780585509588558 86-87 0.0015656970292235442 88-89 0.001222007437442766 90-91 1.5275092968034575E-4 92-93 0.0 94-95 1.5275092968034575E-4 96-97 0.0017948234237440627 98-99 0.0024822026073056187 100-101 7.637546484017287E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1309321.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.01545938289918 #Duplication Level Percentage of deduplicated Percentage of total 1 80.32495977182062 48.20739360618712 2 11.50336229215974 13.807591448237739 3 3.4505631066101357 6.2126138991867315 4 1.5184110685035832 3.6451255123328545 5 0.8078700274183812 2.424234540859475 6 0.4947711672066247 1.7816351333591287 7 0.34351143586584376 1.443119763873755 8 0.22823551295624964 1.095812732604877 9 0.18569917717633752 1.0030339282737857 >10 1.0264505086585787 11.318330853446392 >50 0.08129563852018462 3.3416976878111306 >100 0.03308208542538364 3.5362899807631227 >500 0.0010218329589858812 0.37761345615201375 >1k 5.109164794929406E-4 0.6615517918375718 >5k 2.554582397464703E-4 1.1439556650744014 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8191 0.6255914325058561 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6732 0.5141596293040438 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4534 0.3462863575853438 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1762 0.1345735690483846 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 7.637546484017288E-5 7.637546484017288E-5 5 0.0 0.0 0.0 7.637546484017288E-5 7.637546484017288E-5 6 0.0 0.0 0.0 7.637546484017288E-5 7.637546484017288E-5 7 0.0 0.0 0.0 7.637546484017288E-5 7.637546484017288E-5 8 0.0 0.0 0.0 7.637546484017288E-5 7.637546484017288E-5 9 0.0 0.0 0.0 7.637546484017288E-5 1.5275092968034575E-4 10-11 0.0 0.0 0.0 7.637546484017288E-5 1.5275092968034575E-4 12-13 0.0 0.0 0.0 7.637546484017288E-5 1.5275092968034575E-4 14-15 0.0 0.0 0.0 2.2912639452051865E-4 1.5275092968034575E-4 16-17 3.818773242008644E-5 0.0 0.0 4.582527890410373E-4 1.5275092968034575E-4 18-19 7.637546484017288E-5 0.0 0.0 6.11003718721383E-4 1.5275092968034575E-4 20-21 7.637546484017288E-5 0.0 0.0 6.491914511414695E-4 1.5275092968034575E-4 22-23 7.637546484017288E-5 0.0 0.0 7.255669159816424E-4 1.5275092968034575E-4 24-25 7.637546484017288E-5 0.0 0.0 0.0010692565077624204 1.5275092968034575E-4 26-27 7.637546484017288E-5 0.0 0.0 0.001222007437442766 1.5275092968034575E-4 28-29 7.637546484017288E-5 0.0 0.0 0.0020239498182645812 1.5275092968034575E-4 30-31 1.5275092968034575E-4 0.0 0.0 0.004086087368949249 1.5275092968034575E-4 32-33 1.5275092968034575E-4 0.0 0.0 0.006033661722373658 1.5275092968034575E-4 34-35 1.9093866210043219E-4 0.0 0.0 0.008286737935158758 2.2912639452051865E-4 36-37 3.055018593606915E-4 0.0 0.0 0.011341756528765673 2.2912639452051865E-4 38-39 3.055018593606915E-4 0.0 0.0 0.013938522333331552 2.2912639452051865E-4 40-41 3.055018593606915E-4 0.0 0.0 0.01794823423744063 2.2912639452051865E-4 42-43 3.055018593606915E-4 0.0 0.0 0.02363820636803351 2.2912639452051865E-4 44-45 3.055018593606915E-4 0.0 0.0 0.03383433092419659 2.2912639452051865E-4 46-47 3.055018593606915E-4 0.0 0.0 0.04651265808766529 2.2912639452051865E-4 48-49 3.055018593606915E-4 0.0 0.0 0.07244212840090399 2.2912639452051865E-4 50-51 3.055018593606915E-4 0.0 0.0 0.09722596674154008 2.2912639452051865E-4 52-53 3.055018593606915E-4 0.0 0.0 0.1279289036072896 2.2912639452051865E-4 54-55 3.818773242008644E-4 0.0 0.0 0.18177360631961148 2.2912639452051865E-4 56-57 6.11003718721383E-4 0.0 0.0 0.2393988945415219 2.2912639452051865E-4 58-59 6.11003718721383E-4 0.0 0.0 0.30072839280818076 2.673141269406051E-4 60-61 6.11003718721383E-4 0.0 0.0 0.37007731488305773 3.055018593606915E-4 62-63 6.11003718721383E-4 0.0 0.0 0.4574508466602155 4.582527890410373E-4 64-65 6.11003718721383E-4 0.0 0.0 0.5208042947451389 4.964405214611237E-4 66-67 6.11003718721383E-4 0.0 0.0 0.6208179659533453 5.346282538812102E-4 68-69 6.11003718721383E-4 0.0 0.0 0.7622271391049253 5.346282538812102E-4 70-71 6.491914511414695E-4 0.0 0.0 0.8693055408108478 5.346282538812102E-4 72-73 7.637546484017289E-4 0.0 0.0 1.0118221582026103 6.11003718721383E-4 74-75 9.165055780820746E-4 0.0 0.0 1.1858436548409443 6.11003718721383E-4 76-77 9.165055780820746E-4 0.0 0.0 1.3172476420984618 6.11003718721383E-4 78-79 9.165055780820746E-4 0.0 0.0 1.4438781628034683 6.11003718721383E-4 80-81 9.165055780820746E-4 0.0 0.0 1.6468077728838078 6.11003718721383E-4 82-83 9.165055780820746E-4 0.0 0.0 1.8684875595824095 6.11003718721383E-4 84-85 9.54693310502161E-4 0.0 0.0 2.12938614747644 6.11003718721383E-4 86-87 9.928810429222475E-4 0.0 0.0 2.466774763407904 6.11003718721383E-4 88-89 9.928810429222475E-4 0.0 0.0 2.808478593102837 6.11003718721383E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4880 0.0 39.81911 1 GTATCAA 8235 0.0 38.481346 1 TATCAAC 10150 0.0 30.9823 2 ATCAACG 10245 0.0 30.553574 3 TCAACGC 10385 0.0 30.140528 4 CAACGCA 10440 0.0 30.02724 5 AACGCAG 10765 0.0 29.163712 6 ACGCAGA 12000 0.0 26.04354 7 CGCAGAG 12015 0.0 25.813374 8 GAGTACT 7020 0.0 24.187685 12-13 GCAGAGT 12550 0.0 24.145285 9 GTACATG 6160 0.0 23.292404 1 ACATGGG 5950 0.0 22.911497 3 TACATGG 6200 0.0 22.908741 2 GTACTTT 7545 0.0 22.28517 14-15 AGTACTT 7245 0.0 22.223715 12-13 AGAGTAC 10215 0.0 22.039175 10-11 CATGGGG 2580 0.0 20.066828 4 CAGAGTA 12075 0.0 19.824343 10-11 CATGGGA 3765 0.0 18.797203 4 >>END_MODULE