FastQCFastQC Report
Thu 26 May 2016
SRR936846_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936846_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008999
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54110.5362740696472444No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38020.37680909495450443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25330.2510408830930457No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60000.037.7855151
GGTATCA36300.037.4470561
TATCAAC73600.030.5451012
ATCAACG73850.030.5060583
TCAACGC74800.030.1186144
CAACGCA75350.029.964825
AACGCAG76900.029.2932226
ACGCAGA85000.026.336637
CGCAGAG85150.026.0124388
GAGTACT43200.024.91907512-13
GCAGAGT89250.024.1251379
TACACCG1401.7694687E-523.7668345
GTACATG49900.022.7643681
GTACTTT47350.022.63297714-15
AGTACTT45700.022.56789412-13
TACATGG49650.022.2088952
AGAGTAC71300.022.03130110-11
ACATGGG47550.021.4904843
GTGTAAG4050.019.9504241
CAGAGTA86700.019.70779610-11
Produced by FastQC (version 0.11.5)