##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936846_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1008999 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.973763105810807 33.0 31.0 34.0 30.0 34.0 2 32.08683457565369 33.0 31.0 34.0 30.0 34.0 3 32.101000100099206 34.0 31.0 34.0 30.0 34.0 4 35.677653793512185 37.0 35.0 37.0 33.0 37.0 5 35.688111682965 37.0 35.0 37.0 35.0 37.0 6 35.76327627678521 37.0 35.0 37.0 35.0 37.0 7 35.70531883579667 37.0 35.0 37.0 33.0 37.0 8 35.66193920905769 37.0 35.0 37.0 33.0 37.0 9 37.337802118733514 39.0 37.0 39.0 34.0 39.0 10-11 37.16971225937786 39.0 37.0 39.0 34.0 39.0 12-13 37.120629455529695 39.0 37.0 39.0 33.5 39.0 14-15 38.318859582616035 40.0 38.0 41.0 33.5 41.0 16-17 38.267730691507126 40.0 38.0 41.0 33.0 41.0 18-19 38.24668755865962 40.0 38.0 41.0 33.5 41.0 20-21 38.199498711098826 40.0 38.0 41.0 34.0 41.0 22-23 38.091155194405545 40.0 38.0 41.0 33.0 41.0 24-25 37.94503314671273 40.0 38.0 41.0 33.0 41.0 26-27 38.0298003268586 40.0 38.0 41.0 33.0 41.0 28-29 37.98121355918093 40.0 38.0 41.0 33.0 41.0 30-31 37.83666931285363 40.0 37.5 41.0 33.0 41.0 32-33 37.632350478048046 40.0 37.0 41.0 33.0 41.0 34-35 37.4763176177578 40.0 37.0 41.0 32.5 41.0 36-37 37.30966779947255 40.0 37.0 41.0 32.0 41.0 38-39 37.16836835318965 40.0 36.0 41.0 31.5 41.0 40-41 36.99627898541029 39.5 36.0 41.0 31.0 41.0 42-43 36.77630651764769 39.0 36.0 41.0 31.0 41.0 44-45 36.508452436523726 39.0 36.0 41.0 30.0 41.0 46-47 36.12955364673305 39.0 35.0 41.0 29.5 41.0 48-49 35.787157866360616 39.0 35.0 40.0 28.5 41.0 50-51 35.143933740271294 38.5 34.5 40.0 27.5 40.5 52-53 35.3673333670301 38.5 35.0 40.0 28.0 40.5 54-55 35.749971506413786 39.0 35.0 41.0 28.0 41.0 56-57 35.64950609465421 39.0 35.0 41.0 28.0 41.0 58-59 35.414557397975614 39.0 35.0 41.0 27.5 41.0 60-61 35.15699718235598 38.5 35.0 40.5 27.0 41.0 62-63 34.85542106582861 38.0 34.5 40.0 26.0 41.0 64-65 34.55166308390791 37.5 34.0 40.0 26.0 41.0 66-67 34.18226777231692 37.0 34.0 40.0 26.0 41.0 68-69 33.81182389675312 36.5 34.0 39.0 25.0 41.0 70-71 33.4072015928658 36.0 33.5 39.0 24.5 41.0 72-73 33.01901785829322 35.5 33.0 39.0 23.0 40.0 74-75 32.604516456408774 35.0 33.0 37.5 22.0 40.0 76-77 32.17422613897536 35.0 32.0 37.0 21.0 39.0 78-79 31.741264857546934 35.0 32.0 37.0 20.0 39.0 80-81 31.312133609646786 35.0 31.5 36.0 18.5 38.5 82-83 30.910173845563772 35.0 31.0 36.0 17.5 37.0 84-85 30.561265670233567 34.5 31.0 35.0 13.5 37.0 86-87 30.238526004485635 34.0 31.0 35.0 8.5 36.5 88-89 29.90964213046792 34.0 30.5 35.0 6.0 36.0 90-91 29.58009224984366 34.0 30.0 35.0 2.0 36.0 92-93 29.224184563116516 34.0 30.0 35.0 2.0 35.5 94-95 28.826824407159968 34.0 29.0 35.0 2.0 35.0 96-97 28.377919105965418 34.0 29.0 35.0 2.0 35.0 98-99 27.813730737096865 34.0 29.0 35.0 2.0 35.0 100-101 25.655610164132966 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2963278009286263 1101 2 0.3992886238632849 1101 3 0.35492640819357035 1101 4 0.24698280054109034 1101 5 0.2961781921266251 1101 6 0.2121826943554268 1101 7 0.15476206851599983 1101 8 0.08423520730565315 1101 9 0.10660708159159782 1101 10-11 0.23253129032541864 1101 12-13 0.27601922851250293 1101 14-15 0.47897051224148157 1101 16-17 0.5281503885933603 1101 18-19 0.38544566046477513 1101 20-21 0.45892799408894547 1101 22-23 0.46108290324610834 1101 24-25 0.50602600452914 1101 26-27 0.5150192892544325 1101 28-29 0.534200163213292 1101 30-31 0.5759031886940988 1101 32-33 0.6623811970701823 1101 34-35 0.6228735451549738 1101 36-37 0.4780409923216169 1101 38-39 0.4921350809936271 1101 40-41 0.4678293397754061 1101 42-43 0.5287786857493799 1101 44-45 0.5587776856571836 1101 46-47 0.595026450756059 1101 48-49 0.737198994927283 1101 50-51 0.6740420792636286 1101 52-53 0.683835475359281 1101 54-55 0.8216803846762133 1101 56-57 0.9055098835568032 1101 58-59 0.8833328779045999 1101 60-61 0.8695582849513315 1101 62-63 0.7653108683964263 1101 64-65 1.016555301942141 1101 66-67 0.9719134371452043 1101 68-69 0.9685673312929808 1101 70-71 1.0274551925508675 1101 72-73 1.1741063282439228 1101 74-75 0.9712689711972686 1101 76-77 1.0015999408699017 1101 78-79 0.9046443505466328 1101 80-81 0.7238948662562699 1101 82-83 0.8524287565929782 1101 84-85 0.978389197336476 1101 86-87 1.0269781256990917 1101 88-89 0.9616720415259117 1101 90-91 0.9153838531593905 1101 92-93 1.0747223729156232 1101 94-95 0.7394587987108245 1101 96-97 0.943789457479447 1101 98-99 1.0880248307564386 1101 100-101 1.2008741307702486 1102 1 0.248204625297614 1102 2 0.18824072989345098 1102 3 0.006152026154218504 1102 4 0.11353525723428959 1102 5 0.17868249901266609 1102 6 0.11124177182174577 1102 7 0.10838653250187491 1102 8 0.19344063594668626 1102 9 0.27140211922063884 1102 10-11 0.35040921138956094 1102 12-13 0.2746741179305232 1102 14-15 0.30304065707334615 1102 16-17 0.32348074618059997 1102 18-19 0.24800120466918685 1102 20-21 0.22805161590724055 1102 22-23 0.2884232690498223 1102 24-25 0.19586559539897763 1102 26-27 0.11049310784044764 1102 28-29 0.2397792069055953 1102 30-31 0.18800053959900254 1102 32-33 0.22689127732277115 1102 34-35 0.07076349843192276 1102 36-37 -0.03516715535501902 1102 38-39 0.16455328508545364 1102 40-41 0.16721633433297 1102 42-43 0.18862964522666203 1102 44-45 0.17482763593043416 1102 46-47 0.3012020843900274 1102 48-49 0.5585127763513995 1102 50-51 0.4805930428447951 1102 52-53 0.5629519022985079 1102 54-55 0.5996085651140035 1102 56-57 0.6842551280822775 1102 58-59 0.6855935455788966 1102 60-61 0.7152992920073729 1102 62-63 0.7599339187282723 1102 64-65 0.7385476634952894 1102 66-67 0.5501086314200307 1102 68-69 0.5014684043253723 1102 70-71 0.6909308779635097 1102 72-73 0.7346162216392145 1102 74-75 0.7348774649964938 1102 76-77 0.5386302108787007 1102 78-79 0.6273387367073155 1102 80-81 0.7323942537310693 1102 82-83 0.5810425461375281 1102 84-85 0.6119613849377075 1102 86-87 0.5964252987839593 1102 88-89 0.5631191814305083 1102 90-91 0.5972636724841038 1102 92-93 0.6760077590160591 1102 94-95 0.5418755446172092 1102 96-97 0.5158903970922353 1102 98-99 0.5081324221396954 1102 100-101 0.6728420437052272 1103 1 0.26620410626005864 1103 2 0.20533540214327672 1103 3 0.2193993074695726 1103 4 0.09059039044557693 1103 5 0.1618358225063261 1103 6 0.11469717918105715 1103 7 0.1622842740873054 1103 8 0.31269953448486376 1103 9 0.2359552609509592 1103 10-11 0.35395855653050035 1103 12-13 0.1754021733132376 1103 14-15 0.24896180344494923 1103 16-17 0.2641699338667891 1103 18-19 0.21237448686028415 1103 20-21 0.4124093971118654 1103 22-23 0.4684972369115741 1103 24-25 0.4941498656552241 1103 26-27 0.4899804530435077 1103 28-29 0.36506189700309477 1103 30-31 0.2525741221906017 1103 32-33 0.30814194741227396 1103 34-35 0.2289632397147443 1103 36-37 0.2882440937319046 1103 38-39 0.26000659625447753 1103 40-41 0.14076520853249264 1103 42-43 -0.08163285952031885 1103 44-45 0.13162051489548787 1103 46-47 0.10228595103945537 1103 48-49 0.15955075126324658 1103 50-51 0.2819702458736444 1103 52-53 0.24040964031680545 1103 54-55 0.23386870112589264 1103 56-57 0.2994990005834026 1103 58-59 0.3387043641051335 1103 60-61 0.2762000325877949 1103 62-63 0.4225013911055555 1103 64-65 0.36400420111399256 1103 66-67 0.359110576307863 1103 68-69 0.31138517795585585 1103 70-71 0.19302857125239115 1103 72-73 0.18973450308382667 1103 74-75 0.11997527551972809 1103 76-77 0.38217620932960017 1103 78-79 0.33006022696634574 1103 80-81 0.32419225794148687 1103 82-83 0.4015597600481371 1103 84-85 0.09493409738990266 1103 86-87 0.2763805292897423 1103 88-89 0.23023449494221992 1103 90-91 0.4759281227201271 1103 92-93 0.46182210188746 1103 94-95 0.48409554239276176 1103 96-97 0.6488379091688756 1103 98-99 0.6170907297967041 1103 100-101 0.5463436734631131 1104 1 0.40244009287011195 1104 2 0.3247222284789473 1104 3 0.2694409916691072 1104 4 0.26098556151474384 1104 5 0.32878392582971117 1104 6 0.2914958690888696 1104 7 0.21119535406936052 1104 8 0.3012113326616941 1104 9 0.3746522244103403 1104 10-11 0.41081118998216937 1104 12-13 0.3714281177047525 1104 14-15 0.5547647419229449 1104 16-17 0.4862656563332024 1104 18-19 0.3945159192544665 1104 20-21 0.46076458656701647 1104 22-23 0.2842242330321838 1104 24-25 0.4733885233329147 1104 26-27 0.3549394337266918 1104 28-29 0.5010507536902082 1104 30-31 0.5577859526096773 1104 32-33 0.3934664375018784 1104 34-35 0.474293425329364 1104 36-37 0.4355310990090757 1104 38-39 0.605891640157985 1104 40-41 0.5160450512922878 1104 42-43 0.6391485810831838 1104 44-45 0.5948740562308572 1104 46-47 0.6012373186637348 1104 48-49 0.6694699513152216 1104 50-51 0.564136755579419 1104 52-53 0.6238353589518013 1104 54-55 0.6008511719029244 1104 56-57 0.6973362797983214 1104 58-59 0.6022225834095636 1104 60-61 0.5359363139928774 1104 62-63 0.5200466684041558 1104 64-65 0.7782400990730096 1104 66-67 0.5990782854741994 1104 68-69 0.6216865489500947 1104 70-71 0.5894270928446872 1104 72-73 0.631680830350632 1104 74-75 0.5417200977835179 1104 76-77 0.3998217108667248 1104 78-79 0.6262278308444067 1104 80-81 0.8445504202420899 1104 82-83 0.891044290747196 1104 84-85 0.6842746005269902 1104 86-87 0.8686237464234345 1104 88-89 0.8628251633891928 1104 90-91 0.9887882902948562 1104 92-93 1.0416933393040217 1104 94-95 1.140045756946467 1104 96-97 1.2063581577317848 1104 98-99 1.24780758266089 1104 100-101 1.1783717721960691 1105 1 0.1900621015466264 1105 2 0.23801028230850108 1105 3 0.2917568778966384 1105 4 0.2101209173120111 1105 5 0.24382625198710173 1105 6 0.19622615839343638 1105 7 0.187701984833204 1105 8 0.2540696013375552 1105 9 0.267054027570957 1105 10-11 0.28293553280477113 1105 12-13 0.3226119238048355 1105 14-15 0.25991667347317104 1105 16-17 0.16566487011075282 1105 18-19 0.15686561088809015 1105 20-21 0.1806959733135045 1105 22-23 0.21218532905527354 1105 24-25 0.30003210300463934 1105 26-27 0.26637798330204276 1105 28-29 0.15165368359488696 1105 30-31 -0.12664821552594674 1105 32-33 -0.005444679880916681 1105 34-35 0.10356633149930872 1105 36-37 0.08171596152809713 1105 38-39 0.0072209544726931085 1105 40-41 0.017032841556883227 1105 42-43 0.10714332829061135 1105 44-45 0.24844806801647934 1105 46-47 0.2770400813920446 1105 48-49 0.5281295793015275 1105 50-51 0.415980553629133 1105 52-53 0.29709924623421813 1105 54-55 0.4850738249178832 1105 56-57 0.6909484151988394 1105 58-59 0.6305635275859132 1105 60-61 0.49570603008607605 1105 62-63 0.5133823613301516 1105 64-65 0.5803843981891603 1105 66-67 0.3765609856501655 1105 68-69 0.3294051158599629 1105 70-71 0.2576614235300312 1105 72-73 0.46071253371436427 1105 74-75 0.4011350267566982 1105 76-77 0.6713927060750535 1105 78-79 0.44128126096730824 1105 80-81 0.4994322324092657 1105 82-83 0.5374585581090976 1105 84-85 0.35863191996803323 1105 86-87 0.26263832346259264 1105 88-89 0.381037594587017 1105 90-91 0.5187925159706204 1105 92-93 0.46724973279771476 1105 94-95 0.5092171389429296 1105 96-97 0.23693493162883073 1105 98-99 0.12824447743693668 1105 100-101 0.28641605542738446 1106 1 0.078672360864811 1106 2 0.2639246674852842 1106 3 0.2246050104056465 1106 4 0.13282919673924454 1106 5 -0.06722160929390952 1106 6 0.05721263553082423 1106 7 0.2068466807477165 1106 8 0.26467557018854393 1106 9 0.2907571154961772 1106 10-11 0.3679966141566524 1106 12-13 0.3342349976364787 1106 14-15 0.42471153665717765 1106 16-17 0.44129894354050236 1106 18-19 0.2604038700007436 1106 20-21 0.4147113221020575 1106 22-23 0.15122463509329975 1106 24-25 0.22648353062181314 1106 26-27 0.16819684410776148 1106 28-29 0.21498303475806324 1106 30-31 0.27057371613854286 1106 32-33 0.3191714627725375 1106 34-35 0.34121242851378497 1106 36-37 0.1386706290168931 1106 38-39 0.32130636743475804 1106 40-41 0.47654316308500455 1106 42-43 0.36112825109707103 1106 44-45 0.3098780891752 1106 46-47 0.24401834828552182 1106 48-49 0.3702686469598859 1106 50-51 0.37233633575853986 1106 52-53 0.4620230252953377 1106 54-55 0.4592319262485134 1106 56-57 0.40320267217543915 1106 58-59 0.5141267991313825 1106 60-61 0.5887062191015175 1106 62-63 0.4007837475657894 1106 64-65 0.45491759425691214 1106 66-67 0.508544234844095 1106 68-69 0.48654714720716896 1106 70-71 0.3926909411369124 1106 72-73 0.37180874991867796 1106 74-75 0.4594788660458491 1106 76-77 0.7170614996243074 1106 78-79 0.5247480979313863 1106 80-81 0.6402799391747287 1106 82-83 0.6990897063871273 1106 84-85 0.834721924353925 1106 86-87 0.9088853574798677 1106 88-89 1.0826909292416254 1106 90-91 1.108378039956314 1106 92-93 0.7903854073534831 1106 94-95 1.1216767437721842 1106 96-97 0.9088242970323996 1106 98-99 0.9769059544366954 1106 100-101 0.9386730467051514 1107 1 0.08868442772204688 1107 2 -0.01255745080214865 1107 3 0.06871994973896989 1107 4 0.16259948326162998 1107 5 0.1533611453741912 1107 6 -0.06047044729938733 1107 7 0.056384655087008184 1107 8 0.13798565618756697 1107 9 0.2408438397372663 1107 10-11 0.27818112224588276 1107 12-13 0.3167692793965884 1107 14-15 0.13104975918292183 1107 16-17 0.24712276339582218 1107 18-19 0.24713234153887242 1107 20-21 0.26275655166445233 1107 22-23 0.09721890182524362 1107 24-25 0.18144484214213463 1107 26-27 0.14606201762675397 1107 28-29 0.17084986419636294 1107 30-31 0.1183706585298836 1107 32-33 0.026783856784632576 1107 34-35 0.03827891306961817 1107 36-37 -0.1400551390617366 1107 38-39 -0.1850705334981555 1107 40-41 0.12077685721536113 1107 42-43 0.09862827627832615 1107 44-45 0.09155157492563148 1107 46-47 -4.009820542592024E-4 1107 48-49 0.18946874929650193 1107 50-51 0.10215356013964083 1107 52-53 0.17855260521157135 1107 54-55 0.37896063348319586 1107 56-57 0.3179364257335564 1107 58-59 0.1704701586330728 1107 60-61 0.2218395125477457 1107 62-63 0.14813065057364128 1107 64-65 0.3444539771409083 1107 66-67 0.2855460942279393 1107 68-69 0.1869692857604761 1107 70-71 0.15654163845621838 1107 72-73 0.25167150200822164 1107 74-75 0.2877754448198644 1107 76-77 0.3258586213061747 1107 78-79 0.3047712042506703 1107 80-81 0.3944016069393008 1107 82-83 0.10749188527073272 1107 84-85 0.2189062833701243 1107 86-87 0.32060944747968634 1107 88-89 0.3182988349468907 1107 90-91 0.2920440680263603 1107 92-93 0.12388644784292424 1107 94-95 0.10357041441345771 1107 96-97 0.08396344864571148 1107 98-99 0.2400888448821199 1107 100-101 -0.11790721804048943 1108 1 0.059623864347909006 1108 2 0.06179371820573465 1108 3 0.04332711860728722 1108 4 0.18067865568865926 1108 5 0.08884220269286658 1108 6 -0.00999878827201428 1108 7 0.002016303124271701 1108 8 0.09294363644897174 1108 9 0.10419791763623465 1108 10-11 0.11671149987837737 1108 12-13 0.12736972336227126 1108 14-15 0.2959755977242011 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2316 52-53 -1.1901975167928143 2316 54-55 -1.423902240094236 2316 56-57 -1.4070380346817188 2316 58-59 -1.3002951504772327 2316 60-61 -1.5428237878823623 2316 62-63 -1.4250841812555137 2316 64-65 -1.4707175122235654 2316 66-67 -1.4370411470414268 2316 68-69 -1.4921160717611741 2316 70-71 -1.3754161903212 2316 72-73 -1.8776425730683997 2316 74-75 -1.8498007672530363 2316 76-77 -2.1613439320016106 2316 78-79 -2.2199590847207773 2316 80-81 -2.290725407759787 2316 82-83 -2.068517335007371 2316 84-85 -2.2278493760066453 2316 86-87 -2.5935368797002845 2316 88-89 -2.6709704726538206 2316 90-91 -2.283182209440895 2316 92-93 -2.1128321547351057 2316 94-95 -2.672292075256834 2316 96-97 -3.139671403615111 2316 98-99 -2.9891728496449232 2316 100-101 -3.1745554810233827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1809.0 3 553.0 4 581.0 5 589.0 6 646.0 7 852.0 8 956.0 9 1251.0 10 1529.0 11 2044.0 12 2844.0 13 3560.0 14 4032.0 15 5264.0 16 7066.0 17 10030.0 18 8839.0 19 5217.0 20 4724.0 21 4993.0 22 5321.0 23 5890.0 24 6679.0 25 7579.0 26 8965.0 27 10656.0 28 12681.0 29 15655.0 30 19629.0 31 24554.0 32 31935.0 33 42532.0 34 60001.0 35 88113.0 36 137519.0 37 213495.0 38 209973.0 39 40421.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52727240277349 19.404846774617347 15.063353517486048 22.004527305123112 2 14.857294696911108 20.827458749015946 41.25861853572639 23.05662801834655 3 18.85157085684092 25.924714265745497 30.198460023971048 25.02525485344254 4 12.434159904174896 17.271522968674393 38.33995700562417 31.954360121526538 5 13.344004887492463 37.92686216636517 34.070424323209245 14.658708622933132 6 30.24523241836496 37.153812280068394 18.209345134428546 14.391610167138102 7 27.047683657509207 33.81674566500934 21.47931711313376 17.656253564347697 8 25.669801149916637 35.11778363833466 20.261701745703185 18.95071346604552 9 26.198082433347846 17.35688420710196 21.073297780228653 35.37173557932154 10-11 24.213130778619277 27.510331649289792 27.694027456175107 20.582510115915824 12-13 25.50750382605366 25.088249675913666 28.206917346330513 21.19732915170216 14-15 22.495584015773566 26.13781639621966 27.00603707939052 24.36056250861625 16-17 21.33299947193705 29.56810400608884 27.30596165717558 21.79293486479853 18-19 21.191147408011915 27.77947451245596 30.26099855920783 20.76837952032429 20-21 22.19914975649954 26.850003520369054 30.2438872014256 20.706959521705805 22-23 21.890560334379426 26.87343637615504 30.134448724538988 21.101554564926545 24-25 21.522400901646577 27.105799740489147 30.249618118576038 21.12218123928824 26-27 21.40842836681188 27.334602098418504 30.027655113965835 21.22931442080378 28-29 21.254166992955142 27.403920021490435 30.253682268707728 21.088230716846695 30-31 21.471943557044117 27.091260877193157 30.15555910185285 21.281236463909874 32-33 21.243060094882747 27.316477514038397 30.07147674837538 21.36898564270348 34-35 21.633300161760975 27.329972564070033 29.929893665947727 21.106833608221265 36-37 21.694281429736723 27.155304423152593 29.851795100010804 21.29861904709988 38-39 21.38315533860297 27.497881408429762 29.885273930173206 21.233689322794064 40-41 21.65242532339132 27.293622765797387 29.822869472910064 21.231082437901236 42-43 21.431311215114444 27.401822899631494 29.591691098199895 21.57517478705416 44-45 21.60707525010357 27.595536887674903 29.351613804805236 21.445774057416287 46-47 21.809187019991736 27.782771884511586 28.993663816556936 21.414377278939746 48-49 21.78003019856024 27.88946553900267 28.7243115258499 21.606192736587186 50-51 21.63846587285186 28.120113923178668 28.667924440407145 21.573495763562324 52-53 22.020977409515407 28.235702232783332 28.275099918974572 21.468220438726686 54-55 21.729742320282085 28.09241679737736 28.41994930159603 21.757891580744516 56-57 21.738369695383568 28.355410292403228 28.238680973567128 21.667539038646076 58-59 21.926977969097067 28.0414034677337 28.349659679195483 21.68195888397375 60-61 21.7204124136468 27.991817944811277 28.751537728751092 21.53623191279083 62-63 21.67122881673043 28.070678880640177 28.7923460949631 21.465746207666296 64-65 21.502249275310604 28.14986512277718 28.608194924968817 21.739690676943404 66-67 21.551670302895733 28.32670353449395 28.269260697303505 21.852365465306807 68-69 21.57542683093059 28.257657429918936 28.483780548554265 21.68313519059621 70-71 21.61840806844839 28.16557190416314 28.42820586656569 21.787814160822787 72-73 21.559641894373414 28.23920496308269 28.28604239555872 21.91511074698518 74-75 21.523127439439936 28.504454494764886 28.00475806951242 21.96765999628276 76-77 21.648621976504483 28.36057261221983 28.02917088601634 21.961634525259342 78-79 21.600492562973304 28.441793690672835 27.996077739286658 21.96163600706721 80-81 21.58947153439825 28.59810480480957 27.902674449961136 21.90974921083105 82-83 21.647510433195084 28.68696151750878 27.973454402513386 21.69207364678275 84-85 21.678336582273523 28.470678500640123 27.811225422919822 22.039759494166532 86-87 21.699537995542038 28.288293557464065 27.97027825843006 22.04189018856384 88-89 21.605521411578113 28.211141950832676 28.00297383029342 22.180362807295797 90-91 21.52475102272243 28.30182974880728 28.088958761016507 22.084460467453784 92-93 21.66989861977757 28.410206230333166 27.888830726951515 22.031064422937746 94-95 21.520405285444014 28.383859361470225 27.77425588872791 22.32147946435785 96-97 21.459770861394 28.364565461055808 27.88494190234319 22.290721775207007 98-99 21.483675994079775 28.380491021734766 27.606845446905144 22.528987537280315 100-101 21.4125898538536 28.411272790745652 27.651184563385645 22.524952792015107 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 450.0 1 313.5 2 212.5 3 264.5 4 280.5 5 302.5 6 462.5 7 846.5 8 1344.0 9 1660.0 10 1614.0 11 1491.0 12 1507.5 13 1553.0 14 1684.0 15 1799.0 16 1833.5 17 1890.0 18 2050.0 19 2187.5 20 2348.5 21 2720.5 22 3246.0 23 3855.5 24 4501.5 25 5504.0 26 7049.0 27 8963.0 28 10613.5 29 12228.0 30 14272.5 31 16551.5 32 18453.5 33 20020.5 34 22016.0 35 23969.0 36 26582.0 37 29365.5 38 30973.0 39 31474.5 40 31840.5 41 32941.0 42 33917.5 43 34794.0 44 35995.5 45 37096.5 46 36905.0 47 36827.0 48 38452.0 49 38144.5 50 35768.5 51 34206.0 52 32666.5 53 31298.5 54 29877.5 55 27238.5 56 24467.0 57 21699.0 58 19497.0 59 17519.0 60 14857.5 61 11949.0 62 9148.0 63 6763.0 64 4939.5 65 3656.5 66 2546.0 67 1888.5 68 1344.5 69 835.0 70 532.0 71 358.0 72 242.5 73 160.0 74 123.5 75 96.0 76 58.0 77 30.0 78 18.0 79 11.5 80 9.5 81 10.0 82 8.5 83 5.0 84 5.0 85 4.5 86 4.0 87 2.0 88 0.5 89 2.5 90 3.0 91 1.0 92 0.0 93 1.5 94 1.5 95 1.5 96 1.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04479687294040925 2 0.041328088531306775 3 0.027948491524768606 4 0.049058522357306594 5 0.07046587756776765 6 0.07244804008725479 7 0.07433109448076758 8 0.07452931073271628 9 0.07353822947297271 10-11 0.08032713610221616 12-13 0.07730433825999827 14-15 0.07234893196128044 16-17 0.059117997143703804 18-19 0.05213087426251166 20-21 0.05768092931707564 22-23 0.032160586878678774 24-25 0.017641246423435505 26-27 0.014271570140307374 28-29 0.017641246423435505 30-31 0.012091191368871526 32-33 0.006888014755217794 34-35 0.010208136975358746 36-37 0.005748271306512692 38-39 0.007334001322102401 40-41 0.008126866329897255 42-43 0.005649163180538336 44-45 0.001684838141564065 46-47 0.0031714600311794168 48-49 0.001040635322730746 50-51 0.007284447259115222 52-53 0.0055996091175511565 54-55 0.009068393526653644 56-57 0.025272572123460978 58-59 0.009712596345486962 60-61 0.020416273950717493 62-63 0.015312205463038119 64-65 0.011744312927961277 66-67 0.01684838141564065 68-69 0.02542123431242251 70-71 0.017889016738371396 72-73 0.01888009799811496 74-75 0.018483665494217535 76-77 0.025123909934499443 78-79 0.03934592601181963 80-81 0.03399408720920437 82-83 0.031665046248806984 84-85 0.020664044265653388 86-87 0.024033720548781515 88-89 0.019722517068896998 90-91 0.017046597667589362 92-93 0.03181370843776852 94-95 0.02264620678514052 96-97 0.039197263822858104 98-99 0.05862245651383202 100-101 0.06888014755217794 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1008999.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.01338692014681 #Duplication Level Percentage of deduplicated Percentage of total 1 83.06464875397559 54.00314148828307 2 10.152469818929106 13.20092897066302 3 2.9167584981088854 5.68885046370538 4 1.211470932200231 3.1504731383057836 5 0.6583914959382725 2.1402130535184596 6 0.41611270425176167 1.623173774634504 7 0.2723686105184532 1.2395324102377279 8 0.21344185727317916 1.110126244148476 9 0.13848939169546387 0.8103297965969665 >10 0.872916015008234 10.42799709054143 >50 0.059877077795582954 2.7034979677148536 >100 0.021833396080076724 2.3747712176683518 >500 7.634051407523803E-4 0.361019922287108 >1k 3.053620563009522E-4 0.6288317865517815 >5k 1.526810281504761E-4 0.5371126751431239 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5411 0.5362740696472444 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3802 0.37680909495450443 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2533 0.2510408830930457 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 9.910812597435676E-5 0.0 5 9.910812597435676E-5 0.0 0.0 9.910812597435676E-5 0.0 6 9.910812597435676E-5 0.0 0.0 9.910812597435676E-5 0.0 7 9.910812597435676E-5 0.0 0.0 9.910812597435676E-5 0.0 8 9.910812597435676E-5 0.0 0.0 9.910812597435676E-5 0.0 9 9.910812597435676E-5 0.0 0.0 9.910812597435676E-5 0.0 10-11 9.910812597435676E-5 0.0 0.0 9.910812597435676E-5 0.0 12-13 9.910812597435676E-5 0.0 0.0 9.910812597435676E-5 0.0 14-15 1.9821625194871352E-4 0.0 0.0 1.9821625194871352E-4 0.0 16-17 1.9821625194871352E-4 0.0 0.0 3.9643250389742704E-4 0.0 18-19 1.9821625194871352E-4 0.0 0.0 5.946487558461406E-4 0.0 20-21 1.9821625194871352E-4 0.0 0.0 6.44202818833319E-4 0.0 22-23 1.9821625194871352E-4 0.0 0.0 7.433109448076757E-4 0.0 24-25 2.973243779230703E-4 0.0 0.0 8.424190707820324E-4 4.955406298717838E-5 26-27 2.973243779230703E-4 0.0 0.0 0.0010901893857179243 9.910812597435676E-5 28-29 2.973243779230703E-4 0.0 0.0 0.0016352840785768866 9.910812597435676E-5 30-31 2.973243779230703E-4 0.0 0.0 0.003914770975987092 9.910812597435676E-5 32-33 2.973243779230703E-4 0.0 0.0 0.006491582251320368 9.910812597435676E-5 34-35 2.973243779230703E-4 0.0 0.0 0.008919731337692109 9.910812597435676E-5 36-37 2.973243779230703E-4 0.0 0.0 0.012041637305884346 9.910812597435676E-5 38-39 2.973243779230703E-4 0.0 0.0 0.015411313589012477 9.910812597435676E-5 40-41 3.4687844091024864E-4 0.0 0.0 0.019871179257858532 9.910812597435676E-5 42-43 3.9643250389742704E-4 0.0 0.0 0.03032708654815317 9.910812597435676E-5 44-45 3.9643250389742704E-4 0.0 0.0 0.04717546796379382 9.910812597435676E-5 46-47 3.9643250389742704E-4 0.0 0.0 0.06308232218267808 9.910812597435676E-5 48-49 3.9643250389742704E-4 0.0 0.0 0.09350851685680561 9.910812597435676E-5 50-51 3.9643250389742704E-4 0.0 0.0 0.12477713060171516 9.910812597435676E-5 52-53 3.9643250389742704E-4 0.0 0.0 0.16005962344858618 9.910812597435676E-5 54-55 3.9643250389742704E-4 0.0 0.0 0.2285433384968667 9.910812597435676E-5 56-57 4.955406298717838E-4 0.0 0.0 0.300297621702301 9.910812597435676E-5 58-59 4.955406298717838E-4 0.0 0.0 0.379187689977889 9.910812597435676E-5 60-61 4.955406298717838E-4 0.0 0.0 0.4699211793074126 9.910812597435676E-5 62-63 4.955406298717838E-4 0.0 0.0 0.5836973079259742 9.910812597435676E-5 64-65 4.955406298717838E-4 0.0 0.0 0.6573841995879084 9.910812597435676E-5 66-67 4.955406298717838E-4 0.0 0.0 0.7834497358272903 9.910812597435676E-5 68-69 5.946487558461406E-4 0.0 0.0 0.9693270260921965 9.910812597435676E-5 70-71 5.946487558461406E-4 0.0 0.0 1.0916264535445526 9.910812597435676E-5 72-73 5.946487558461406E-4 0.0 0.0 1.27537291910101 1.9821625194871352E-4 74-75 5.946487558461406E-4 0.0 0.0 1.4953929587640822 1.9821625194871352E-4 76-77 5.946487558461406E-4 0.0 0.0 1.6667509085737449 1.9821625194871352E-4 78-79 5.946487558461406E-4 0.0 0.0 1.8299819920535105 1.9821625194871352E-4 80-81 5.946487558461406E-4 0.0 0.0 2.0783469557452485 1.9821625194871352E-4 82-83 5.946487558461406E-4 0.0 0.0 2.353768437827986 1.9821625194871352E-4 84-85 5.946487558461406E-4 0.0 0.0 2.69440306680185 1.9821625194871352E-4 86-87 5.946487558461406E-4 0.0 0.0 3.111945601531815 1.9821625194871352E-4 88-89 5.946487558461406E-4 0.0 0.0 3.5305783256474985 1.9821625194871352E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6000 0.0 37.785515 1 GGTATCA 3630 0.0 37.447056 1 TATCAAC 7360 0.0 30.545101 2 ATCAACG 7385 0.0 30.506058 3 TCAACGC 7480 0.0 30.118614 4 CAACGCA 7535 0.0 29.96482 5 AACGCAG 7690 0.0 29.293222 6 ACGCAGA 8500 0.0 26.33663 7 CGCAGAG 8515 0.0 26.012438 8 GAGTACT 4320 0.0 24.919075 12-13 GCAGAGT 8925 0.0 24.125137 9 TACACCG 140 1.7694687E-5 23.766834 5 GTACATG 4990 0.0 22.764368 1 GTACTTT 4735 0.0 22.632977 14-15 AGTACTT 4570 0.0 22.567894 12-13 TACATGG 4965 0.0 22.208895 2 AGAGTAC 7130 0.0 22.031301 10-11 ACATGGG 4755 0.0 21.490484 3 GTGTAAG 405 0.0 19.950424 1 CAGAGTA 8670 0.0 19.707796 10-11 >>END_MODULE