FastQCFastQC Report
Thu 26 May 2016
SRR936846_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936846_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1008999
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68740.6812692579477284No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54480.5399410703082956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36670.36342949794796625No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14270.1414272957654071No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37700.043.8553731
GTATCAA63450.037.8881871
ATCAACG80850.029.6107463
TATCAAC81550.029.3594862
TCAACGC82700.028.945484
CAACGCA83500.028.6681585
AACGCAG85600.027.9620846
GTACATG48850.025.6758171
GCACCGT1501.0616186E-625.3286156
TACATGG48250.025.1064172
ACGCAGA95250.024.97967
CGCAGAG95650.024.7261858
ACATGGG47150.024.379933
CGGTATC1000.001652436923.752642
TGCGCGA500.001655871723.74557754-55
GAGTACT61050.023.6123712-13
GCAGAGT99800.023.3648879
GTACTTT63850.022.4314614-15
AGAGTAC85500.022.21808210-11
AGTACTT63100.022.09252212-13
Produced by FastQC (version 0.11.5)