##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936846_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1008999 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20575144276654 33.0 31.0 34.0 31.0 34.0 2 32.276137042752275 33.0 31.0 34.0 30.0 34.0 3 32.40427393882452 34.0 31.0 34.0 31.0 34.0 4 36.08797134585862 37.0 35.0 37.0 35.0 37.0 5 36.00607136379719 37.0 35.0 37.0 35.0 37.0 6 36.04887517232425 37.0 35.0 37.0 35.0 37.0 7 35.99899900792766 37.0 35.0 37.0 35.0 37.0 8 35.98659364379945 37.0 35.0 37.0 35.0 37.0 9 37.68164487774517 39.0 37.0 39.0 35.0 39.0 10-11 37.58418937977144 39.0 37.0 39.0 35.0 39.0 12-13 37.504959866164384 39.0 37.0 39.0 35.0 39.0 14-15 38.72180596809313 40.0 38.0 41.0 35.0 41.0 16-17 38.648797967094126 40.0 38.0 41.0 34.0 41.0 18-19 38.70162259823845 40.0 38.0 41.0 34.5 41.0 20-21 38.79756768837234 40.0 38.0 41.0 35.0 41.0 22-23 38.73585404940937 40.0 38.0 41.0 34.5 41.0 24-25 38.64874643086861 40.0 38.0 41.0 34.0 41.0 26-27 38.473626336596965 40.0 38.0 41.0 34.0 41.0 28-29 38.31492746771801 40.0 38.0 41.0 34.0 41.0 30-31 38.23864691639932 40.0 38.0 41.0 34.0 41.0 32-33 38.1242013123898 40.0 37.0 41.0 34.0 41.0 34-35 37.99750197968481 40.0 37.0 41.0 33.5 41.0 36-37 37.822968109978305 40.0 37.0 41.0 33.0 41.0 38-39 37.67762604323691 40.0 37.0 41.0 33.0 41.0 40-41 37.444376555378156 40.0 37.0 41.0 33.0 41.0 42-43 37.20112457990543 40.0 36.0 41.0 32.0 41.0 44-45 36.90693102768189 40.0 36.0 41.0 31.0 41.0 46-47 36.478843883888885 39.5 36.0 41.0 30.5 41.0 48-49 36.05458380038038 39.0 35.5 41.0 29.5 41.0 50-51 35.8079036748302 39.0 35.0 40.5 28.5 41.0 52-53 35.58385835863068 39.0 35.0 40.0 28.0 41.0 54-55 35.324929459791335 39.0 35.0 40.0 27.0 41.0 56-57 35.07690245480917 38.0 35.0 40.0 27.0 41.0 58-59 34.73301707930335 38.0 34.0 40.0 26.0 41.0 60-61 34.42088693844097 38.0 34.0 40.0 26.0 41.0 62-63 34.09270326333326 37.0 34.0 40.0 25.0 41.0 64-65 33.69386689184033 37.0 33.0 39.5 24.0 41.0 66-67 33.25972325046903 36.0 33.0 39.0 23.0 40.0 68-69 32.824726783673725 36.0 32.0 39.0 22.0 40.0 70-71 32.43055840491418 35.0 32.0 38.0 21.0 40.0 72-73 32.05061402439448 35.0 31.5 37.5 20.0 40.0 74-75 31.57884596515953 35.0 31.0 37.0 18.5 39.0 76-77 30.138687451622843 33.5 29.5 35.5 17.5 38.0 78-79 30.82178228125102 34.0 31.0 36.0 17.5 38.5 80-81 30.47727946212038 34.0 31.0 36.0 9.5 37.5 82-83 30.303321410625777 34.0 30.0 35.0 10.5 37.0 84-85 29.982413758586482 34.0 30.0 35.0 7.0 36.5 86-87 29.614497635775656 34.0 30.0 35.0 4.5 36.0 88-89 29.241912529150177 34.0 29.5 35.0 2.0 36.0 90-91 28.795417042038693 34.0 29.0 35.0 2.0 35.0 92-93 28.37091463916218 34.0 29.0 35.0 2.0 35.0 94-95 27.871569743874872 33.5 28.0 35.0 2.0 35.0 96-97 27.249308473051016 33.0 27.0 35.0 2.0 35.0 98-99 26.467147638402018 33.0 25.5 34.5 2.0 35.0 100-101 23.300081070447046 29.0 11.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2202093062424808 1101 2 0.21447782545718042 1101 3 0.2084855080725987 1101 4 0.1388876552249414 1101 5 0.19092632456602843 1101 6 0.244458068000867 1101 7 0.12737928480265737 1101 8 0.15257846295548205 1101 9 0.25957107516705946 1101 10-11 0.16146462282512175 1101 12-13 0.21747798200310342 1101 14-15 0.23875143780397678 1101 16-17 0.34876672373226825 1101 18-19 0.2556730315659408 1101 20-21 0.09824669101229944 1101 22-23 0.2889457981754262 1101 24-25 0.22942872211893928 1101 26-27 0.17919995224535512 1101 28-29 0.2803472586604343 1101 30-31 0.3700655363398724 1101 32-33 0.37636525386674435 1101 34-35 0.3892230671541128 1101 36-37 0.5210969368836658 1101 38-39 0.4247142427270987 1101 40-41 0.5530014834496484 1101 42-43 0.610132459300047 1101 44-45 0.48932600162246587 1101 46-47 0.6275677603322976 1101 48-49 0.5424855834122795 1101 50-51 0.4055204795977474 1101 52-53 0.5289735506707132 1101 54-55 0.5761909032598211 1101 56-57 0.5572894253438534 1101 58-59 0.5570583603624897 1101 60-61 0.565417441419747 1101 62-63 0.5580436141780396 1101 64-65 0.5855606199194341 1101 66-67 0.4866138778776232 1101 68-69 0.44171415537809366 1101 70-71 0.39933066560373476 1101 72-73 0.40806076955086823 1101 74-75 0.4130503457271786 1101 76-77 0.35133988769759483 1101 78-79 0.4912372059048131 1101 80-81 0.9132080016258577 1101 82-83 0.5988268747982808 1101 84-85 0.40843837479190626 1101 86-87 0.42488236772948085 1101 88-89 0.4862836605423482 1101 90-91 0.3565295373820625 1101 92-93 0.5033418361778175 1101 94-95 0.5718376045689624 1101 96-97 0.4354235210989863 1101 98-99 0.6870390583699475 1101 100-101 1.045479586935592 1102 1 0.26501183562666597 1102 2 0.19596236301592995 1102 3 0.14222364066967685 1102 4 0.11313714813440612 1102 5 0.1788246951529402 1102 6 0.18238520937237723 1102 7 0.1278761156505226 1102 8 0.18277468931126606 1102 9 0.20196401859732305 1102 10-11 0.24949951274957982 1102 12-13 0.26481487261037273 1102 14-15 0.20260852166012455 1102 16-17 0.23361984429276816 1102 18-19 0.25365820347902 1102 20-21 0.08956634010477416 1102 22-23 0.19211944710549034 1102 24-25 0.2182936862886109 1102 26-27 0.21987132003204124 1102 28-29 0.12259080357036822 1102 30-31 0.25798870481094127 1102 32-33 0.21886223489898526 1102 34-35 0.32204939726809556 1102 36-37 0.3014902802234758 1102 38-39 0.08647022980076713 1102 40-41 0.19527344987820783 1102 42-43 0.4154031447956399 1102 44-45 0.41806963079280735 1102 46-47 0.5177122603973459 1102 48-49 0.6809551348412484 1102 50-51 0.6945000903233591 1102 52-53 0.6770699637964697 1102 54-55 0.45466380764214875 1102 56-57 0.5032800265964923 1102 58-59 0.4473681068914388 1102 60-61 0.5499229113614703 1102 62-63 0.4950438909713242 1102 64-65 0.5162086218124529 1102 66-67 0.3780692168009594 1102 68-69 0.16319134961974413 1102 70-71 0.3444649103466304 1102 72-73 0.42890059704840766 1102 74-75 0.4350861908940793 1102 76-77 0.49097074848978295 1102 78-79 0.5004709250897328 1102 80-81 0.3825407027877823 1102 82-83 0.4295402767449161 1102 84-85 0.5738027159584043 1102 86-87 0.33015161250293446 1102 88-89 0.24000465291176099 1102 90-91 0.3405541117359867 1102 92-93 0.3629094099755328 1102 94-95 0.676521566886656 1102 96-97 0.6278265059259986 1102 98-99 0.6092167055222504 1102 100-101 0.9468643392056038 1103 1 0.1842749425774599 1103 2 0.09684723186876454 1103 3 0.14835389504818153 1103 4 0.12991568843646917 1103 5 0.18244443100491026 1103 6 0.1425721230127479 1103 7 0.1777508151748748 1103 8 0.09565603912955112 1103 9 0.15944502312537878 1103 10-11 0.13942271923935579 1103 12-13 0.15257350211074794 1103 14-15 0.31453075420297694 1103 16-17 0.1442792524496781 1103 18-19 0.16326400547720965 1103 20-21 0.13861914666508568 1103 22-23 0.18666579525544336 1103 24-25 0.1657626768006466 1103 26-27 0.17257001472272293 1103 28-29 0.12517747432520565 1103 30-31 0.20824326367411317 1103 32-33 0.3180793198517975 1103 34-35 0.25169150183512556 1103 36-37 0.2813109417258204 1103 38-39 0.19536090225499692 1103 40-41 0.2278262729715692 1103 42-43 0.1267947165475718 1103 44-45 0.353129816103241 1103 46-47 0.5171796958007207 1103 48-49 0.4394228117273471 1103 50-51 0.38700440645129675 1103 52-53 0.4343580326461023 1103 54-55 0.34955319152052056 1103 56-57 0.4935833930259861 1103 58-59 0.6040079799977818 1103 60-61 0.6096821200195848 1103 62-63 0.7914939947287394 1103 64-65 0.4516325342133456 1103 66-67 0.5212807466491185 1103 68-69 0.6417993369864945 1103 70-71 0.6976374021577598 1103 72-73 0.6876793958672458 1103 74-75 0.6383126906671244 1103 76-77 0.6903608292612446 1103 78-79 0.7465458288222919 1103 80-81 0.9379013687590572 1103 82-83 0.8626515662125627 1103 84-85 0.7771361795521265 1103 86-87 0.7811063209651472 1103 88-89 0.8472628119717207 1103 90-91 0.8291533215564186 1103 92-93 0.8254515316768121 1103 94-95 0.7726760296577346 1103 96-97 1.0897235881934506 1103 98-99 1.1370030003255636 1103 100-101 1.3923979609153996 1104 1 0.11701249242089062 1104 2 0.16573272449221577 1104 3 0.1155584791564479 1104 4 0.1460726420470806 1104 5 0.036555197429485986 1104 6 0.06267933003779547 1104 7 -0.01960155505611283 1104 8 0.10742294521471507 1104 9 0.2593377310924936 1104 10-11 0.1922805916025183 1104 12-13 0.2531875622853619 1104 14-15 0.23537297540915603 1104 16-17 0.3104049049607198 1104 18-19 0.12976697133989035 1104 20-21 0.0694956321011162 1104 22-23 0.0979182217737602 1104 24-25 0.16492231362288834 1104 26-27 0.1179664457294507 1104 28-29 0.04949165842911185 1104 30-31 0.2864018967966828 1104 32-33 0.2973055299535261 1104 34-35 0.21618494317698378 1104 36-37 0.3310511517073138 1104 38-39 0.4010507195913746 1104 40-41 0.4541863528060688 1104 42-43 0.5917062142824179 1104 44-45 0.7217377581424742 1104 46-47 0.6943454373999742 1104 48-49 0.9593674716261731 1104 50-51 0.8988982170914852 1104 52-53 0.877374244247612 1104 54-55 0.8321135303860459 1104 56-57 0.7021270448533201 1104 58-59 0.7740112042235552 1104 60-61 0.8687152651819972 1104 62-63 0.7333455989784028 1104 64-65 0.9123513240417935 1104 66-67 0.8302386902367047 1104 68-69 1.1141720939181141 1104 70-71 1.0546253802281669 1104 72-73 0.9827816694018132 1104 74-75 0.9626843765000253 1104 76-77 0.9994474990003361 1104 78-79 1.2127026913530834 1104 80-81 0.9742695425539374 1104 82-83 1.013893864654431 1104 84-85 0.786503132270159 1104 86-87 0.7648236771971639 1104 88-89 0.678391709960497 1104 90-91 0.6717443118187703 1104 92-93 0.7611473542095979 1104 94-95 0.8477558611236304 1104 96-97 0.9651476406748607 1104 98-99 1.1923567634706558 1104 100-101 1.464933022093721 1105 1 0.06538136615492363 1105 2 -0.03516345805697796 1105 3 -0.012619067083086577 1105 4 0.017084660547524777 1105 5 0.038615599917392274 1105 6 0.07846238322733257 1105 7 -0.030179624415801243 1105 8 0.07205295954191371 1105 9 0.030232849511655502 1105 10-11 0.002947955351459086 1105 12-13 0.09263578773287406 1105 14-15 0.144523259871157 1105 16-17 -0.021312955613574047 1105 18-19 0.1085614816627185 1105 20-21 0.15387343218964133 1105 22-23 -0.10195508506428297 1105 24-25 0.09191335488183228 1105 26-27 0.031214787460157822 1105 28-29 0.06441646761825126 1105 30-31 0.09437346447612782 1105 32-33 -0.012540988567728562 1105 34-35 0.10870087042750498 1105 36-37 -0.09913921961238259 1105 38-39 0.026481962140536552 1105 40-41 0.19175183921373673 1105 42-43 0.1874490001748299 1105 44-45 0.13267855704194886 1105 46-47 0.36579182143987055 1105 48-49 0.41988130762985776 1105 50-51 0.4084167830760279 1105 52-53 0.24881538474824083 1105 54-55 0.3344334109567271 1105 56-57 0.11629223484680296 1105 58-59 0.2882740165804165 1105 60-61 0.33711137447586736 1105 62-63 0.3063129178488211 1105 64-65 0.2340509401838773 1105 66-67 0.11846447703748453 1105 68-69 0.1414693424480049 1105 70-71 0.10818042273938744 1105 72-73 0.12931237417975794 1105 74-75 0.3745755668913908 1105 76-77 0.29408312699898786 1105 78-79 0.26543920196276716 1105 80-81 0.5752011189931672 1105 82-83 0.5750308336111338 1105 84-85 0.35291024374317814 1105 86-87 0.47323971194023784 1105 88-89 0.33564930808022453 1105 90-91 0.1982345950778459 1105 92-93 0.4417973063237497 1105 94-95 0.4305706238457674 1105 96-97 0.5204762332318076 1105 98-99 0.8252272773529299 1105 100-101 0.7064951649739974 1106 1 0.16339505234156348 1106 2 0.12814864315253516 1106 3 0.06507637580947545 1106 4 0.09753917847555016 1106 5 0.14530656332730274 1106 6 0.02896786192272316 1106 7 -0.010006354136550044 1106 8 0.09472472045021618 1106 9 0.11658630106222745 1106 10-11 0.10474103298176374 1106 12-13 0.00834590776686639 1106 14-15 0.0848035532671787 1106 16-17 0.08986574840107409 1106 18-19 0.1801010767292368 1106 20-21 0.21225076208032334 1106 22-23 0.23167507592916792 1106 24-25 0.24467224640714136 1106 26-27 0.19129573378527454 1106 28-29 0.21017674097496553 1106 30-31 0.08480524549714374 1106 32-33 -9.757204318887602E-4 1106 34-35 0.07275765580688187 1106 36-37 0.3336855153530962 1106 38-39 0.24108836040526427 1106 40-41 0.3634512716168601 1106 42-43 0.20034288176307058 1106 44-45 0.2558377208963165 1106 46-47 0.4056038887000142 1106 48-49 0.31792582352091614 1106 50-51 0.11436104370447708 1106 52-53 0.14781461217053504 1106 54-55 0.08066870032470064 1106 56-57 0.13643568472742373 1106 58-59 0.0619523306360108 1106 60-61 0.17928897926474718 1106 62-63 0.2593118406442656 1106 64-65 0.2062987009013213 1106 66-67 0.27600977192911813 1106 68-69 0.3727269925271841 1106 70-71 0.2829664957874414 1106 72-73 0.29248959543243913 1106 74-75 0.2643745131761399 1106 76-77 0.20546928923296548 1106 78-79 0.12099699022509469 1106 80-81 0.7476378034052651 1106 82-83 0.6248405335903975 1106 84-85 0.12946003279826712 1106 86-87 0.21390867982412942 1106 88-89 -0.018695936896644838 1106 90-91 0.17141871631199024 1106 92-93 0.2574338387894457 1106 94-95 0.4906454225810144 1106 96-97 0.5009434133598631 1106 98-99 0.48817878112681257 1106 100-101 0.47293354501983487 1107 1 -0.033916194009670164 1107 2 -0.15307057276363167 1107 3 -0.0541911115646414 1107 4 0.01411468900030144 1107 5 0.09632600124140822 1107 6 -0.07736854755268041 1107 7 -0.01710948245418109 1107 8 0.05696693994615032 1107 9 -0.12420189235398027 1107 10-11 -0.18918766579201218 1107 12-13 0.06012007824597276 1107 14-15 -0.06686616536754997 1107 16-17 -0.012990611678780795 1107 18-19 -0.06108397326058679 1107 20-21 0.09584185073646978 1107 22-23 0.01787816576984369 1107 24-25 -0.014955554503806923 1107 26-27 -0.04669784398753052 1107 28-29 -0.061439855284909584 1107 30-31 -0.0299563081661276 1107 32-33 0.07373555130205744 1107 34-35 -0.021463044796057318 1107 36-37 0.08317584675855016 1107 38-39 -0.08102269497133108 1107 40-41 0.1254989911017077 1107 42-43 0.05492063241225509 1107 44-45 0.1911692371503193 1107 46-47 0.2685413122749054 1107 48-49 0.1660224228428362 1107 50-51 0.14907894314464443 1107 52-53 0.20921859806097132 1107 54-55 -0.030772956722728395 1107 56-57 7.656316856312628E-4 1107 58-59 0.09955046198546569 1107 60-61 0.084016709466745 1107 62-63 0.24381882189531012 1107 64-65 0.08101994441211957 1107 66-67 -0.063045749715684 1107 68-69 -0.01910088453188763 1107 70-71 0.14694591968062554 1107 72-73 -0.1541659310471637 1107 74-75 0.04901132897626681 1107 76-77 -0.025999321829463184 1107 78-79 0.12588265725638692 1107 80-81 0.2932531426169156 1107 82-83 0.14626575231477545 1107 84-85 0.15738050435251338 1107 86-87 -0.014519546549305318 1107 88-89 0.10267468886760511 1107 90-91 0.020412271925291492 1107 92-93 -0.021323657670070162 1107 94-95 -0.08655677886499547 1107 96-97 0.0840264450217667 1107 98-99 0.12924316254425605 1107 100-101 0.3037296151325073 1108 1 0.13683178786536132 1108 2 -0.035888490137168105 1108 3 0.12096163792619308 1108 4 0.12053779963846978 1108 5 0.028987571170368653 1108 6 0.13195385963101103 1108 7 0.11998345814251365 1108 8 0.16228667810234043 1108 9 0.0971682084323362 1108 10-11 0.16888396183503573 1108 12-13 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-1.0595474778539113 2316 62-63 -1.1772280733370835 2316 64-65 -1.0488455904876446 2316 66-67 -0.9732863098296818 2316 68-69 -1.251191454080896 2316 70-71 -0.9159042329061933 2316 72-73 -0.7915444960153124 2316 74-75 -0.9235515114159689 2316 76-77 -1.0897872814606515 2316 78-79 -0.8415250911608112 2316 80-81 -0.5655829030094743 2316 82-83 -0.8028210041904948 2316 84-85 -1.1382141612904029 2316 86-87 -0.9917958230405262 2316 88-89 -0.9545038865115814 2316 90-91 -1.0780721082598923 2316 92-93 -1.0123880564649923 2316 94-95 -0.9589434449243512 2316 96-97 -1.1535844162966633 2316 98-99 -1.5803619987292734 2316 100-101 -2.1646631153581772 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 6.0 5 14.0 6 35.0 7 106.0 8 280.0 9 547.0 10 907.0 11 1498.0 12 2400.0 13 3361.0 14 4407.0 15 6155.0 16 8448.0 17 12319.0 18 11157.0 19 6279.0 20 5089.0 21 5080.0 22 5303.0 23 5780.0 24 6535.0 25 7832.0 26 9527.0 27 11781.0 28 14695.0 29 18323.0 30 23065.0 31 28877.0 32 36924.0 33 48632.0 34 67076.0 35 96813.0 36 148358.0 37 227877.0 38 171127.0 39 12386.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59899058894208 19.828850201297303 15.675909852910708 21.89624935684991 2 15.316293093707442 21.11207585245801 40.58148132729907 22.990149726535474 3 19.054982000412327 25.646339819528365 30.904281047306405 24.394397132752907 4 12.793087796011488 17.863662762463356 37.84061018951475 31.50263925201041 5 13.284383737606909 37.75617511242119 34.16484212466883 14.794599025303068 6 30.340565253285686 37.23125592790478 17.81498296826855 14.613195850540981 7 26.99199900099009 33.97277896211988 21.220734609251345 17.814487427638678 8 25.918412046814808 34.89148134236276 19.95565893654788 19.234447674274552 9 26.048614566133928 16.93709997443008 21.06991292351422 35.94437253592177 10-11 24.292045879133674 27.362812054323143 27.585904445891423 20.759237620651756 12-13 25.707285841993738 24.864766828080928 28.354070077443165 21.073877252482166 14-15 22.625939173378764 25.806764922462754 27.414298725766823 24.15299717839165 16-17 21.137186459054966 29.092843501331515 27.958204121114093 21.81176591849942 18-19 20.882588773773687 26.935717747618543 31.36224021027989 20.81945326832788 20-21 21.85015451155913 26.038022331330662 31.46936627465594 20.642456882454265 22-23 21.418356212444216 26.22415879500376 31.42505592176008 20.932429070791944 24-25 21.296393115386312 26.159440723012295 31.724557966708804 20.819608194892588 26-27 21.221628639958766 26.382440977659723 31.510397050369694 20.885533332011814 28-29 20.998394322642035 26.619305792332394 31.564891170756848 20.817408714268723 30-31 21.2957785576904 26.282592746812604 31.434973132671068 20.98665556282593 32-33 21.049986942433225 26.3344610247737 31.45758619852358 21.157965834269497 34-35 21.29993453645517 26.35727832215101 31.39821905459937 20.94456808679445 36-37 21.407751519966613 26.133810589881364 31.320506154954074 21.137931735197952 38-39 21.20814608801835 26.50347993807276 31.38793132499311 20.900442648915778 40-41 21.29261474157468 26.476781591194843 31.186574401940316 21.044029265290163 42-43 21.27022073945885 26.486901430926125 30.90243397193256 21.340443857682466 44-45 21.426582131696552 26.62227160146327 30.615746141365808 21.33540012547437 46-47 21.59308050934809 27.011098380932747 30.167565928228225 21.228255181490937 48-49 21.721745283860365 27.178978415868666 29.66509808896492 21.434178211306048 50-51 21.55287671993173 27.350519683759522 29.546947954318174 21.54965564199057 52-53 21.8993008684138 27.519212738585843 29.121001104100635 21.460485288899726 54-55 21.614943767607468 27.37495447146011 29.222261040206348 21.787840720726077 56-57 21.69930391166264 27.586247906953005 29.168564180910618 21.545884000473738 58-59 21.88326940802188 27.201805766162202 29.365206790949365 21.54971803486655 60-61 21.649685306767054 27.118325745776627 29.801134491280212 21.4308544561761 62-63 21.52194553408431 27.351838160234216 29.785242388983924 21.340973916697557 64-65 21.365885133390588 27.39962068933049 29.517099953366692 21.71739422391223 66-67 21.467751246912144 27.586627254414676 29.160523988037575 21.78509751063561 68-69 21.56907246942738 27.332999005937612 29.322808627628987 21.775119897006018 70-71 21.61619995926521 27.348705183675754 29.324739052029038 21.710355805030005 72-73 21.5848802257955 27.356393615676826 29.11379230877882 21.944933849748853 74-75 21.545143328357824 27.541474138255715 28.910730250103573 22.002652283282885 76-77 21.530865029577324 27.62066691081112 28.83671443671761 22.011753622893945 78-79 21.501561865794024 27.604261913718002 28.848104772623763 22.04607144786421 80-81 21.70769586034448 27.72753536432715 28.61108605872961 21.95368271659876 82-83 21.608033726267944 27.7211427959758 28.800040438219472 21.870783039536786 84-85 21.50590812121993 27.662841894265817 28.694712795189258 22.136537189324994 86-87 21.438425607954024 27.483377089570947 28.776936349788258 22.30126095268677 88-89 21.35611194390416 27.444393513299225 28.702238630310088 22.497255912486526 90-91 21.346548469788488 27.51907963819426 28.76031534364162 22.374056548375634 92-93 21.25025086781682 27.31448789516327 28.88827772120347 22.54698351581644 94-95 21.231267213944992 27.356433592700842 28.776562374565533 22.635736818788637 96-97 21.29105502348125 27.249202549265533 28.79747352939282 22.6622688978604 98-99 21.155148858443866 27.299774875110938 28.527877478145342 23.017198788299847 100-101 20.817539907461963 27.423548586433665 28.5634998545576 23.195411651546767 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 533.0 1 403.5 2 289.5 3 316.5 4 268.0 5 246.5 6 490.0 7 1145.0 8 1924.0 9 2276.5 10 1992.5 11 1644.5 12 1654.0 13 1791.5 14 1944.0 15 2067.5 16 2170.5 17 2255.0 18 2438.0 19 2583.0 20 2734.0 21 2972.5 22 3382.5 23 4039.0 24 4782.5 25 5857.0 26 7271.5 27 9013.5 28 10480.0 29 12068.5 30 14233.0 31 16332.5 32 18070.5 33 19436.0 34 21607.0 35 23910.5 36 26378.5 37 29046.5 38 30342.0 39 30670.0 40 31298.0 41 32598.5 42 33461.5 43 34113.5 44 35553.5 45 36991.5 46 36931.0 47 36780.5 48 38308.0 49 37861.0 50 35340.5 51 34014.5 52 32786.0 53 31484.0 54 29786.0 55 27063.0 56 24133.5 57 21440.5 58 19691.0 59 17854.0 60 15146.0 61 12186.0 62 9444.0 63 7074.5 64 4976.0 65 3521.0 66 2540.5 67 1940.5 68 1405.5 69 842.5 70 543.5 71 361.0 72 237.5 73 174.0 74 115.5 75 81.0 76 60.5 77 27.5 78 12.0 79 8.0 80 7.0 81 4.0 82 1.5 83 1.5 84 1.0 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.028939572784512174 2 0.009217055715615178 3 0.008622406959769038 4 9.910812597435677E-5 5 0.006937568818204973 6 0.0 7 0.0 8 1.9821625194871355E-4 9 9.910812597435677E-5 10-11 0.0 12-13 9.910812597435677E-5 14-15 0.0 16-17 0.0 18-19 0.005748271306512692 20-21 0.0012884056376666379 22-23 0.0 24-25 0.005302284739628087 26-27 0.0058473794324870485 28-29 0.007829541951974184 30-31 0.03359765470530694 32-33 5.450946928589622E-4 34-35 0.0040138791019614485 36-37 0.017591692360448326 38-39 0.0072348931961280435 40-41 0.008969285400679287 42-43 0.007135785070153687 44-45 0.002775027527281989 46-47 0.0022794868974102054 48-49 0.001684838141564065 50-51 0.001982162519487135 52-53 0.0032705681571537732 54-55 0.0016352840785768866 56-57 3.4687844091024864E-4 58-59 8.919731337692109E-4 60-61 4.955406298717839E-5 62-63 9.910812597435677E-5 64-65 0.006293365999371654 66-67 6.442028188333189E-4 68-69 7.928650077948542E-4 70-71 0.0033201222201409515 72-73 0.012041637305884346 74-75 0.005649163180538336 76-77 0.0033201222201409515 78-79 0.010852339794192065 80-81 1.9821625194871355E-4 82-83 0.00520317661365373 84-85 6.442028188333189E-4 86-87 0.0 88-89 0.0011397434487051027 90-91 4.4598656688460544E-4 92-93 1.4866218896153515E-4 94-95 5.946487558461406E-4 96-97 2.477703149358919E-4 98-99 3.4687844091024864E-4 100-101 8.424190707820325E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1008999.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.51075770039017 #Duplication Level Percentage of deduplicated Percentage of total 1 82.04003410511312 51.28384693676472 2 10.62638104470724 13.285262614354265 3 3.11408114372162 5.839907155036082 4 1.3007650966050497 3.252472471160115 5 0.7373591693608577 2.3046440187038764 6 0.4594536855049091 1.7232478805489189 7 0.2944853801340222 1.2885952970705608 8 0.21281298269279744 1.064248063728543 9 0.16975036014406333 0.9550101269267561 >10 0.9481698652308208 10.912777361328132 >50 0.0657563657303786 2.7608275994074005 >100 0.029514939526227607 3.212196667883835 >500 7.977008493695708E-4 0.3800701497530301 >1k 3.190803397478283E-4 0.508023443411686 >5k 3.190803397478283E-4 1.2288702139220251 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6874 0.6812692579477284 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5448 0.5399410703082956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3667 0.36342949794796625 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1427 0.1414272957654071 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.910812597435676E-5 0.0 0.0 0.0 0.0 8 1.9821625194871352E-4 0.0 0.0 0.0 0.0 9 1.9821625194871352E-4 0.0 0.0 0.0 0.0 10-11 1.9821625194871352E-4 0.0 0.0 9.910812597435676E-5 0.0 12-13 1.9821625194871352E-4 0.0 0.0 9.910812597435676E-5 0.0 14-15 1.9821625194871352E-4 0.0 0.0 1.9821625194871352E-4 0.0 16-17 1.9821625194871352E-4 0.0 0.0 3.4687844091024864E-4 0.0 18-19 1.9821625194871352E-4 0.0 0.0 4.955406298717838E-4 0.0 20-21 1.9821625194871352E-4 0.0 0.0 6.44202818833319E-4 0.0 22-23 1.9821625194871352E-4 0.0 0.0 7.433109448076757E-4 4.955406298717838E-5 24-25 1.9821625194871352E-4 0.0 0.0 7.928650077948541E-4 9.910812597435676E-5 26-27 2.973243779230703E-4 0.0 0.0 9.910812597435678E-4 9.910812597435676E-5 28-29 3.9643250389742704E-4 0.0 0.0 0.0016352840785768866 9.910812597435676E-5 30-31 3.9643250389742704E-4 0.0 0.0 0.003865216912999914 9.910812597435676E-5 32-33 3.9643250389742704E-4 0.0 0.0 0.006590690377294725 9.910812597435676E-5 34-35 3.9643250389742704E-4 0.0 0.0 0.009068393526653645 1.9821625194871352E-4 36-37 3.9643250389742704E-4 0.0 0.0 0.012140745431858703 1.9821625194871352E-4 38-39 3.9643250389742704E-4 0.0 0.0 0.015807746092909903 2.973243779230703E-4 40-41 3.9643250389742704E-4 0.0 0.0 0.020317165824743137 2.973243779230703E-4 42-43 5.450946928589621E-4 0.0 0.0 0.03092173530399931 2.973243779230703E-4 44-45 5.946487558461406E-4 0.0 0.0 0.04806744109756303 2.973243779230703E-4 46-47 7.433109448076757E-4 0.0 0.0 0.06382563312748576 3.4687844091024864E-4 48-49 7.928650077948541E-4 0.0 0.0 0.09449959811654918 3.9643250389742704E-4 50-51 7.928650077948541E-4 0.0 0.0 0.12542133342054848 3.9643250389742704E-4 52-53 7.928650077948541E-4 0.0 0.0 0.16055516407845796 4.955406298717838E-4 54-55 7.928650077948541E-4 0.0 0.0 0.2287911088118026 5.946487558461406E-4 56-57 7.928650077948541E-4 0.0 0.0 0.3004958379542497 5.946487558461406E-4 58-59 7.928650077948541E-4 0.0 0.0 0.37923724404087616 5.946487558461406E-4 60-61 7.928650077948541E-4 0.0 0.0 0.4692274224255921 5.946487558461406E-4 62-63 7.928650077948541E-4 0.0 0.0 0.5828548888551921 5.946487558461406E-4 64-65 7.928650077948541E-4 0.0 0.0 0.6558480236353059 5.946487558461406E-4 66-67 7.928650077948541E-4 0.0 0.0 0.781715343622739 5.946487558461406E-4 68-69 7.928650077948541E-4 0.0 0.0 0.9674935257616707 5.946487558461406E-4 70-71 7.928650077948541E-4 0.0 0.0 1.0893965207101295 6.937568818204974E-4 72-73 7.928650077948541E-4 0.0 0.0 1.2705166209282663 7.928650077948541E-4 74-75 7.928650077948541E-4 0.0 0.0 1.4882571736939283 7.928650077948541E-4 76-77 8.919731337692109E-4 0.0 0.0 1.6586240422438476 8.424190707820324E-4 78-79 8.919731337692109E-4 0.0 0.0 1.8211613688417927 8.919731337692109E-4 80-81 8.919731337692109E-4 0.0 0.0 2.0691299000296333 8.919731337692109E-4 82-83 8.919731337692109E-4 0.0 0.0 2.344105395545486 8.919731337692109E-4 84-85 9.910812597435678E-4 0.0 0.0 2.684888686708312 8.919731337692109E-4 86-87 0.0010901893857179245 0.0 0.0 3.1014401401785334 8.919731337692109E-4 88-89 0.0010901893857179245 0.0 0.0 3.519329553349409 8.919731337692109E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3770 0.0 43.855373 1 GTATCAA 6345 0.0 37.888187 1 ATCAACG 8085 0.0 29.610746 3 TATCAAC 8155 0.0 29.359486 2 TCAACGC 8270 0.0 28.94548 4 CAACGCA 8350 0.0 28.668158 5 AACGCAG 8560 0.0 27.962084 6 GTACATG 4885 0.0 25.675817 1 GCACCGT 150 1.0616186E-6 25.328615 6 TACATGG 4825 0.0 25.106417 2 ACGCAGA 9525 0.0 24.9796 7 CGCAGAG 9565 0.0 24.726185 8 ACATGGG 4715 0.0 24.37993 3 CGGTATC 100 0.0016524369 23.75264 2 TGCGCGA 50 0.0016558717 23.745577 54-55 GAGTACT 6105 0.0 23.61237 12-13 GCAGAGT 9980 0.0 23.364887 9 GTACTTT 6385 0.0 22.43146 14-15 AGAGTAC 8550 0.0 22.218082 10-11 AGTACTT 6310 0.0 22.092522 12-13 >>END_MODULE