FastQCFastQC Report
Thu 26 May 2016
SRR936845_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936845_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079661
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56700.5251648434091812No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40700.3769701786023576No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.2376671936839434No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34900.039.218481
GTATCAA58900.037.1971241
ATCAACG71350.030.5747243
TATCAAC72800.029.965752
TCAACGC72500.029.9586354
CAACGCA73300.029.6316665
GCGTATC650.005828805729.246261
AACGCAG75150.029.0892436
ACGCAGA83800.025.8006537
CGCAGAG84950.025.1716948
GCAGAGT88400.023.7033319
GTACATG52600.023.4915311
GAGTACT45000.023.12087812-13
TACATGG53550.022.6320862
ACATGGG52850.021.9426293
AGAGTAC73050.021.20314610-11
GTACTTT49250.021.17096514-15
AGTACTT46750.020.93429412-13
CAGAGTA86550.019.3780710-11
CATGGGA32950.019.184044
Produced by FastQC (version 0.11.5)