FastQCFastQC Report
Thu 26 May 2016
SRR936845_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936845_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079661
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72910.6753045631915944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58340.5403547965518807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38380.35548195220536816No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14460.13393092831916686No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGGCG352.786995E-454.2891545
GGTATCA38400.047.134081
GTATCAA62950.037.6570321
TATCAAC81850.028.6687852
ATCAACG82500.028.4415933
TCAACGC84000.027.9902524
CAACGCA84450.027.7874345
AACGCAG87100.027.0510836
ACGCAGA98500.023.7273927
CGCAGAG98700.023.5349278
GTACATG50250.023.256281
GAGTACT62650.023.08377612-13
TACATGG49850.023.059572
ACATGGG48600.022.3810333
GCAGAGT103700.022.1253179
GTACTTT66600.021.46609514-15
AGTACTT64200.021.45377212-13
AGAGTAC88600.021.36563110-11
ACACCGT1856.5479E-620.5418426
CATGGGG18850.019.906584
Produced by FastQC (version 0.11.5)