##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936845_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1079661 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.21071799388882 33.0 31.0 34.0 31.0 34.0 2 32.3323802563953 34.0 31.0 34.0 31.0 34.0 3 32.426968279858215 34.0 31.0 34.0 31.0 34.0 4 36.08187847852243 37.0 35.0 37.0 35.0 37.0 5 35.99949706435631 37.0 35.0 37.0 35.0 37.0 6 36.04431946694379 37.0 35.0 37.0 35.0 37.0 7 35.99408888530752 37.0 35.0 37.0 35.0 37.0 8 35.965348382501546 37.0 35.0 37.0 35.0 37.0 9 37.651762914470375 39.0 37.0 39.0 35.0 39.0 10-11 37.565262614839284 39.0 37.0 39.0 35.0 39.0 12-13 37.464926953923495 39.0 37.0 39.0 35.0 39.0 14-15 38.66584557560197 40.0 38.0 41.0 34.5 41.0 16-17 38.57803375318734 40.0 38.0 41.0 34.0 41.0 18-19 38.74942412479473 40.0 38.0 41.0 35.0 41.0 20-21 38.78786813638726 40.0 38.0 41.0 35.0 41.0 22-23 38.70531305659832 40.0 38.0 41.0 34.0 41.0 24-25 38.59973964049827 40.0 38.0 41.0 34.0 41.0 26-27 38.42032591711658 40.0 38.0 41.0 34.0 41.0 28-29 38.33108725794486 40.0 38.0 41.0 34.0 41.0 30-31 38.29545987120031 40.0 38.0 41.0 34.0 41.0 32-33 38.10583229365514 40.0 37.0 41.0 34.0 41.0 34-35 37.944070870393574 40.0 37.0 41.0 33.0 41.0 36-37 37.77866015351115 40.0 37.0 41.0 33.0 41.0 38-39 37.60702665003181 40.0 37.0 41.0 33.0 41.0 40-41 37.42498524999977 40.0 37.0 41.0 33.0 41.0 42-43 37.14786307924432 40.0 36.0 41.0 32.0 41.0 44-45 36.83756382790524 40.0 36.0 41.0 31.0 41.0 46-47 36.401224087931304 39.0 36.0 41.0 30.5 41.0 48-49 35.97062596500198 39.0 35.0 41.0 29.5 41.0 50-51 35.669927875509075 39.0 35.0 40.0 28.0 41.0 52-53 35.4250278559659 39.0 35.0 40.0 27.5 41.0 54-55 35.13334555939318 38.5 35.0 40.0 26.5 41.0 56-57 34.85065497410761 38.0 34.0 40.0 26.0 41.0 58-59 34.50094937207142 38.0 34.0 40.0 26.0 41.0 60-61 34.17791834659212 37.5 34.0 40.0 25.0 41.0 62-63 33.817445012832735 37.0 33.0 40.0 24.0 41.0 64-65 33.434905030375276 36.5 33.0 39.0 23.0 40.5 66-67 33.03857414503256 36.0 32.0 39.0 22.0 40.0 68-69 32.732626722647204 36.0 32.0 39.0 22.0 40.0 70-71 32.2633044075872 35.0 31.5 38.0 20.0 40.0 72-73 31.742414980257692 35.0 31.0 37.5 18.0 39.5 74-75 31.235997225054902 35.0 31.0 37.0 17.5 39.0 76-77 29.852472211184807 33.5 29.5 35.5 14.0 37.5 78-79 30.587979004520864 34.0 30.0 36.0 14.0 38.0 80-81 30.33877439307338 34.0 30.0 36.0 9.0 37.5 82-83 30.00319127948495 34.0 30.0 35.0 7.5 37.0 84-85 29.641674562663653 34.0 30.0 35.0 6.0 36.5 86-87 29.291134439421263 34.0 29.0 35.0 2.0 36.0 88-89 28.897831819432213 34.0 29.0 35.0 2.0 36.0 90-91 28.435529763509102 34.0 29.0 35.0 2.0 35.0 92-93 27.940729080702184 33.0 27.5 35.0 2.0 35.0 94-95 27.325198372452093 33.0 26.5 35.0 2.0 35.0 96-97 26.6364808027705 32.5 25.0 34.0 2.0 35.0 98-99 25.78626994954898 32.0 24.5 34.0 2.0 35.0 100-101 22.23181998794066 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.14293489114659508 1101 2 0.1322767943979386 1101 3 0.10781906081212611 1101 4 0.10813493402722685 1101 5 0.18807972941974072 1101 6 0.19307258628018786 1101 7 0.13979728263784352 1101 8 0.2344602885175462 1101 9 0.1985972030655745 1101 10-11 0.22410947277759163 1101 12-13 0.2431896229152457 1101 14-15 0.31887716287705103 1101 16-17 0.26672530679869055 1101 18-19 0.1465558296469709 1101 20-21 0.28934073171028274 1101 22-23 0.25994344219675725 1101 24-25 0.1937641645330075 1101 26-27 0.14172914528800362 1101 28-29 0.16042360183442383 1101 30-31 0.26962860947032397 1101 32-33 0.3665229178130076 1101 34-35 0.3636529113135438 1101 36-37 0.34069808640738586 1101 38-39 0.2715593519788939 1101 40-41 0.3814094089203124 1101 42-43 0.4843203553740949 1101 44-45 0.378011432830327 1101 46-47 0.3563271091592526 1101 48-49 0.6050237919692663 1101 50-51 0.5140917476135414 1101 52-53 0.5754532116484228 1101 54-55 0.48163737765671755 1101 56-57 0.5718888175231882 1101 58-59 0.37562472913754874 1101 60-61 0.557893314907659 1101 62-63 0.5214764339518396 1101 64-65 0.49541809859545083 1101 66-67 0.24032379034247953 1101 68-69 0.25771759531509275 1101 70-71 0.13378295983554267 1101 72-73 0.3743707781173491 1101 74-75 0.3846684007061434 1101 76-77 0.4430423499070706 1101 78-79 0.49533639821641273 1101 80-81 0.7626727278697629 1101 82-83 0.638441419808025 1101 84-85 0.5819885480189839 1101 86-87 0.29996586761702915 1101 88-89 0.23424923464781955 1101 90-91 0.5361831166652635 1101 92-93 0.6428137837682648 1101 94-95 0.49459897494648075 1101 96-97 0.40658217054913237 1101 98-99 0.6346277670701248 1101 100-101 1.1181336723082076 1102 1 0.17318889204319277 1102 2 0.2911114109594095 1102 3 0.23173122254156908 1102 4 0.20104770772128688 1102 5 0.17623269922711415 1102 6 0.19789447611232447 1102 7 0.10973896810226336 1102 8 0.1329884602376623 1102 9 0.18434901227492873 1102 10-11 0.1853362216799681 1102 12-13 0.19937099926637103 1102 14-15 0.2470515959202899 1102 16-17 0.3282055140127582 1102 18-19 0.30316495859361225 1102 20-21 0.3866344338115937 1102 22-23 0.26844556984119095 1102 24-25 0.19738213850316555 1102 26-27 0.1619544425533732 1102 28-29 0.19653751976000677 1102 30-31 0.31607832519141965 1102 32-33 0.2697546780651976 1102 34-35 0.33272067927932625 1102 36-37 0.15577247390908155 1102 38-39 0.23667025277657672 1102 40-41 0.2319523940979593 1102 42-43 0.3688115886251211 1102 44-45 0.2115641720835839 1102 46-47 0.23812832205889833 1102 48-49 0.2145612338859948 1102 50-51 0.14590986069890732 1102 52-53 0.10916063207523763 1102 54-55 0.18758548697068278 1102 56-57 0.12394050007010549 1102 58-59 0.20594486312933924 1102 60-61 0.3075406347160907 1102 62-63 0.31277003643101153 1102 64-65 0.3322806058002641 1102 66-67 0.43067880987553764 1102 68-69 0.17959114990446778 1102 70-71 0.11182939620901067 1102 72-73 0.38210861709948674 1102 74-75 0.518106263170651 1102 76-77 0.37949209231266323 1102 78-79 0.5488601758882439 1102 80-81 0.6390990145965354 1102 82-83 0.3258320133425485 1102 84-85 0.3555921034622678 1102 86-87 0.30326607362140123 1102 88-89 0.3399699030412684 1102 90-91 0.5035063851967649 1102 92-93 0.35267562030255206 1102 94-95 0.13570840987543065 1102 96-97 0.3433494585496426 1102 98-99 0.5248392684470495 1102 100-101 0.7745955917423579 1103 1 0.1605543980581956 1103 2 0.19302956318079367 1103 3 0.18057387946775805 1103 4 0.12144612754103434 1103 5 0.07612847879594398 1103 6 0.04242439233895112 1103 7 -0.07211972884457651 1103 8 0.014702737444160618 1103 9 0.010700403865826047 1103 10-11 0.11149827948757007 1103 12-13 0.2213853320570962 1103 14-15 0.24293116673062798 1103 16-17 0.23788610320558945 1103 18-19 0.25202200786505813 1103 20-21 0.1896009939418235 1103 22-23 0.10066407541946631 1103 24-25 0.013643007205274671 1103 26-27 0.056293536133395605 1103 28-29 0.149603585587613 1103 30-31 0.16784213191710506 1103 32-33 0.11922613159281781 1103 34-35 0.035223458490825976 1103 36-37 -0.02355490727963172 1103 38-39 0.05368696774858961 1103 40-41 0.09432360742228951 1103 42-43 -0.019016953876963782 1103 44-45 -0.050384452206721164 1103 46-47 0.04252716868613504 1103 48-49 0.12557063357631648 1103 50-51 0.25494105734797046 1103 52-53 0.13592692023976838 1103 54-55 0.22511303094177748 1103 56-57 0.3588166266433319 1103 58-59 0.22529140102587064 1103 60-61 0.11389758472999745 1103 62-63 0.20413934401031497 1103 64-65 0.14500443158340914 1103 66-67 0.07122427976079138 1103 68-69 -0.06420574928956313 1103 70-71 0.09213496058746529 1103 72-73 0.24392994357743447 1103 74-75 0.25765617979430644 1103 76-77 0.020433103548615605 1103 78-79 0.13407675586998025 1103 80-81 0.27013238498602377 1103 82-83 0.2923588922880356 1103 84-85 0.3301352705722813 1103 86-87 0.29244377441943215 1103 88-89 0.19104659935285895 1103 90-91 0.05315476531349006 1103 92-93 -0.033936272737509654 1103 94-95 -0.05262485728620092 1103 96-97 -0.043018260908809225 1103 98-99 -0.29391693515004746 1103 100-101 0.1319168288885031 1104 1 0.148808423952417 1104 2 0.17183652975853647 1104 3 0.22789856277339737 1104 4 0.053822278045927874 1104 5 0.07180160272225322 1104 6 0.03228737925217473 1104 7 0.028976655183129196 1104 8 -0.0029371737277870125 1104 9 0.1588477243591342 1104 10-11 0.16689709790681917 1104 12-13 0.11227347295292134 1104 14-15 0.041910202750685244 1104 16-17 0.12267080690257615 1104 18-19 0.1413821944598439 1104 20-21 0.05830167411892262 1104 22-23 0.04531693369960976 1104 24-25 0.04230541869146265 1104 26-27 -0.017766157437741015 1104 28-29 -0.13740461285090788 1104 30-31 -0.024099096312333756 1104 32-33 -0.04365463489833843 1104 34-35 0.03593436227439639 1104 36-37 0.14466392259229366 1104 38-39 0.14422454508905247 1104 40-41 0.1298520365903002 1104 42-43 0.1049903646610062 1104 44-45 -0.031607653648080714 1104 46-47 0.08039222266732793 1104 48-49 -0.04574181492156271 1104 50-51 0.15178458868221156 1104 52-53 0.13876215536875236 1104 54-55 0.08768513390410959 1104 56-57 -0.03046394990102641 1104 58-59 0.0624385678857422 1104 60-61 0.20457343215802837 1104 62-63 0.21520514101865018 1104 64-65 0.1521022960787235 1104 66-67 0.13666234453823733 1104 68-69 0.05309616833498865 1104 70-71 0.1865371170421568 1104 72-73 0.11169764983031527 1104 74-75 0.009686236080007404 1104 76-77 0.04138172014940977 1104 78-79 0.007178335500977795 1104 80-81 0.29887440652263564 1104 82-83 0.29544552757623777 1104 84-85 0.3139227536376872 1104 86-87 0.13037586844309246 1104 88-89 -0.04856847907002759 1104 90-91 0.009535441021249369 1104 92-93 -0.11411197351545255 1104 94-95 0.08088538727028549 1104 96-97 0.10114995373106339 1104 98-99 -0.3658383307002637 1104 100-101 -0.4045068641798366 1105 1 0.1703193544934436 1105 2 0.158080386236513 1105 3 0.09925997727862068 1105 4 0.13959069023574955 1105 5 0.14598561269221477 1105 6 0.06756380850853816 1105 7 0.12913001353047093 1105 8 0.12179227742306864 1105 9 -0.04038849093059582 1105 10-11 0.05775607170893693 1105 12-13 0.1193219428776402 1105 14-15 0.1493124994506374 1105 16-17 0.19349396367891103 1105 18-19 0.020032306889220308 1105 20-21 0.10436163422570388 1105 22-23 0.12136129068042578 1105 24-25 0.05722060384076855 1105 26-27 0.12739503588398549 1105 28-29 0.10090724251780614 1105 30-31 0.05907959790314976 1105 32-33 -0.029155087622875442 1105 34-35 -0.041611503231330005 1105 36-37 0.059470520676484284 1105 38-39 0.19360225767746897 1105 40-41 0.09773799678395534 1105 42-43 0.13185747748129728 1105 44-45 0.09989796447960941 1105 46-47 0.02934393984646988 1105 48-49 -0.04157942213041821 1105 50-51 0.052622880824849005 1105 52-53 7.456568171306799E-4 1105 54-55 -0.09071305653422002 1105 56-57 -0.14385490677024393 1105 58-59 -0.1351878859279978 1105 60-61 -0.10616131804160034 1105 62-63 0.06061491519096762 1105 64-65 -0.1488863473848383 1105 66-67 -0.3349252651764587 1105 68-69 -0.19355617597621944 1105 70-71 -0.3340800584245116 1105 72-73 -0.5961012402428203 1105 74-75 -0.31944282883501174 1105 76-77 -0.4414434814048924 1105 78-79 0.0269415154648307 1105 80-81 0.005337842751959698 1105 82-83 0.0820228298726029 1105 84-85 0.08892901827606181 1105 86-87 -0.2537984856309272 1105 88-89 -0.1569851460151206 1105 90-91 -0.23342962067290784 1105 92-93 -0.321735453714183 1105 94-95 -0.4027032648802411 1105 96-97 -0.15784222465910958 1105 98-99 -0.30728684873740875 1105 100-101 0.3792167700454705 1106 1 0.11916199136247485 1106 2 0.14058959516346192 1106 3 0.06616491400388469 1106 4 0.014137185290387322 1106 5 0.1007693185988856 1106 6 0.03479884747294193 1106 7 0.06723507242440974 1106 8 0.03244409051733044 1106 9 0.10093537330174485 1106 10-11 0.052209120064283354 1106 12-13 0.11262040466291268 1106 14-15 0.22607900919019386 1106 16-17 0.0166640557990263 1106 18-19 -0.053279312709598514 1106 20-21 0.1518250144726352 1106 22-23 0.057704775943051345 1106 24-25 0.037203142357832064 1106 26-27 0.0865448837901468 1106 28-29 0.11736299339241896 1106 30-31 0.1565708208856691 1106 32-33 0.16657047401368885 1106 34-35 -0.046933630570585194 1106 36-37 0.023024688294384532 1106 38-39 0.20380303388049015 1106 40-41 0.26892801535606026 1106 42-43 0.2106624456742523 1106 44-45 0.09983510622613068 1106 46-47 0.08363650298238667 1106 48-49 0.08461218525153669 1106 50-51 0.2999608530715818 1106 52-53 0.33151157113863405 1106 54-55 0.22598610617841786 1106 56-57 0.19837357642538933 1106 58-59 0.1565850870282972 1106 60-61 -0.03928746337297184 1106 62-63 0.3702338476341609 1106 64-65 0.12864942397462897 1106 66-67 0.14909562690170475 1106 68-69 0.07177313957067355 1106 70-71 0.1345806674302068 1106 72-73 0.08136997607595475 1106 74-75 0.2291674253128626 1106 76-77 0.1100316436946045 1106 78-79 0.02394321533710908 1106 80-81 0.11681676248466744 1106 82-83 0.26930880330399987 1106 84-85 0.18998735073529005 1106 86-87 0.10326146031868788 1106 88-89 0.27693424733717364 1106 90-91 0.27386386489823167 1106 92-93 0.31265009336086536 1106 94-95 0.07516137469130868 1106 96-97 -0.03220670072207099 1106 98-99 0.003587320608122724 1106 100-101 0.18365201756045124 1107 1 0.07884168741896502 1107 2 0.07615636001540338 1107 3 0.10114596855453328 1107 4 0.07167846051980575 1107 5 -0.021578488558887443 1107 6 -0.05803895635152401 1107 7 -0.06348767776096054 1107 8 -0.06514538166312889 1107 9 0.008395186500294471 1107 10-11 0.090511580591766 1107 12-13 0.1256160645563824 1107 14-15 0.13439000707456472 1107 16-17 0.1392244465446666 1107 18-19 0.09503472937174706 1107 20-21 0.007053465299492245 1107 22-23 0.1141848339018452 1107 24-25 0.05652103815446452 1107 26-27 0.03576886866100182 1107 28-29 0.12612823988733624 1107 30-31 0.14234642785432783 1107 32-33 0.11186293801053893 1107 34-35 -0.14340618858039278 1107 36-37 -0.1780406956145697 1107 38-39 -0.04751668503298845 1107 40-41 0.022684367353662083 1107 42-43 -0.14127053437351123 1107 44-45 -0.19816074074089585 1107 46-47 -0.283539570649765 1107 48-49 -0.3427046357224697 1107 50-51 -0.5811296667208055 1107 52-53 -0.4233672222029128 1107 54-55 -0.024010492484173085 1107 56-57 -0.2114378364221352 1107 58-59 -0.1317637167213377 1107 60-61 -0.10876105538935121 1107 62-63 -0.2727702815894517 1107 64-65 -0.29151778577224263 1107 66-67 -0.4122832072558893 1107 68-69 -0.3333928967147983 1107 70-71 -0.26173763992824917 1107 72-73 -0.5162046152533577 1107 74-75 -0.4389653594981233 1107 76-77 -0.24927798685212466 1107 78-79 -0.07585840165869584 1107 80-81 0.1135412745628166 1107 82-83 0.019481421177349745 1107 84-85 -0.25096458156448875 1107 86-87 -0.23189569847615132 1107 88-89 -0.0773949749130125 1107 90-91 0.025263940407921126 1107 92-93 -0.04543930739151136 1107 94-95 0.028372577336188698 1107 96-97 0.1814644647802055 1107 98-99 -0.08518971152090771 1107 100-101 0.23244505298197637 1108 1 0.09979963322389551 1108 2 -0.06321827332224217 1108 3 -0.05141811430821974 1108 4 0.03249067244409076 1108 5 0.09869670820716436 1108 6 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2632.0 13 3674.0 14 4869.0 15 6722.0 16 9295.0 17 13460.0 18 12155.0 19 6772.0 20 5664.0 21 5739.0 22 6125.0 23 6485.0 24 7613.0 25 8978.0 26 10716.0 27 13487.0 28 16768.0 29 20695.0 30 26237.0 31 32844.0 32 41805.0 33 54830.0 34 75693.0 35 107483.0 36 164252.0 37 241812.0 38 160280.0 39 8854.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.58187646497494 19.91772978682009 15.623928773265886 21.876464974939086 2 15.41252434909305 21.05428909910903 40.46455707575613 23.06862947604179 3 19.01324308253169 25.70841877705718 30.852640373155204 24.425697767255926 4 12.832641598735888 17.871853271375155 37.84854948765946 31.446955642229497 5 13.331604393975882 37.79875145879259 34.09517811162773 14.774466035603801 6 30.325629989413343 37.26299273568278 17.759556008784237 14.651821266119644 7 27.010885824346715 33.97205233865074 21.261303316503977 17.755758520498564 8 25.882846560170275 34.89484199206973 20.019061538760777 19.203249908999215 9 26.103378745735927 16.95384014056264 20.996868461489303 35.94591265221213 10-11 24.297419684429965 27.347611965668662 27.62263689530295 20.732331454598423 12-13 25.70492034073658 24.955333201810568 28.313516927998695 21.026229529454152 14-15 22.584311186566893 25.83523902410108 27.307784573120635 24.272665216211383 16-17 21.19276328403082 29.136969845164362 27.825724926620488 21.844541944184332 18-19 20.965595938330274 26.96024515250017 31.294038972652828 20.780119936516726 20-21 21.936830191021034 26.090601035247218 31.39725030114051 20.575318472591235 22-23 21.568737327940173 26.146332246241567 31.34302299194453 20.94190743387373 24-25 21.32659715583617 26.209214260836056 31.58842992967401 20.875758653653765 26-27 21.263682163469955 26.387714327264106 31.38845997449053 20.960143534775412 28-29 20.964011651870273 26.612251135779484 31.493625278445432 20.93011193390481 30-31 21.31146174401534 26.281845253256886 31.384958805522235 21.02173419720554 32-33 21.097006744562044 26.320325710059322 31.371545571296316 21.21112197408232 34-35 21.41445324041528 26.374760225663422 31.24642827702399 20.964358256897302 36-37 21.468772172933598 26.154474602207152 31.23005014903916 21.146703075820085 38-39 21.290938456315253 26.4936176559273 31.273416479736248 20.942027408021204 40-41 21.42779075012481 26.42683210345604 31.11378070795362 21.031596438465535 42-43 21.27181587553871 26.495353634149975 30.834910217188543 21.397920273122768 44-45 21.45465104324413 26.658553008768493 30.484892943247928 21.40190300473945 46-47 21.64276323071273 27.031884678136052 30.04094357788797 21.284408513263244 48-49 21.764259921611707 27.170579057274953 29.5784973585748 21.48666366253854 50-51 21.628623763132847 27.317344275733667 29.432586832531722 21.621445128601763 52-53 21.98537318658357 27.47542978768336 29.049766547092098 21.48943047864098 54-55 21.69906828932585 27.344629209903047 29.146918333831962 21.80938416693914 56-57 21.71503597305552 27.59224825229072 29.04270222831486 21.6500135463389 58-59 21.894330475793964 27.198553987209845 29.288659330705038 21.618456206291157 60-61 21.74595433959975 27.1265680508723 29.655641278217043 21.471836331310907 62-63 21.56001139302941 27.342714829231916 29.633269498405674 21.464004279333 64-65 21.51545716664768 27.345250036817113 29.395060116091997 21.74423268044321 66-67 21.509163432963348 27.53221083421441 29.154828279123358 21.803797453698877 68-69 21.647235350099454 27.3172279171867 29.327124574228403 21.708412158485444 70-71 21.639403935632753 27.30522943346839 29.283465868752657 21.7719007621462 72-73 21.63410552015864 27.324224918747642 29.060603281956094 21.981066279137618 74-75 21.60580960134709 27.61778928756341 28.766807358976564 22.009593752112934 76-77 21.59909453059803 27.552676256713916 28.810524785094582 22.03770442759348 78-79 21.61602670500015 27.632385740537657 28.732096016479446 22.01949153798275 80-81 21.693410834224444 27.73586836766025 28.605553592458993 21.96516720565631 82-83 21.70823400600341 27.627533049543167 28.73183218802959 21.932400756423835 84-85 21.579842690062 27.721164128739794 28.58755905606627 22.111434125131936 86-87 21.525423571822888 27.468257585020023 28.67936919012845 22.326949653028635 88-89 21.489218828149603 27.415204786692104 28.62303873441639 22.4725376507419 90-91 21.441201124433267 27.395279047473498 28.7721540677346 22.391365760358635 92-93 21.46387683906191 27.255373332635585 28.80979715382752 22.470952674474987 94-95 21.322248372405785 27.335154275277144 28.64412996301617 22.698467389300898 96-97 21.32111507436268 27.188204227145874 28.758309475094034 22.732371223397408 98-99 21.250996277938043 27.229543826272284 28.50922549897258 23.010234396817097 100-101 20.900551608584365 27.303443999294224 28.50854504726254 23.28745934485887 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 566.0 1 414.5 2 289.5 3 325.0 4 286.0 5 272.5 6 494.0 7 1119.5 8 1897.0 9 2270.5 10 2039.0 11 1694.0 12 1728.0 13 1921.0 14 2030.0 15 2124.0 16 2245.5 17 2372.0 18 2476.5 19 2639.0 20 2887.5 21 3212.5 22 3684.0 23 4332.0 24 5069.5 25 6159.0 26 7697.5 27 9524.5 28 11261.0 29 13001.5 30 15259.5 31 17538.0 32 19162.5 33 20660.0 34 22936.5 35 25195.0 36 27998.0 37 30968.5 38 32606.0 39 33218.0 40 33595.0 41 34429.5 42 35462.0 43 36509.0 44 37888.0 45 39271.5 46 39121.5 47 39294.5 48 41187.5 49 40639.0 50 38032.5 51 36619.0 52 35317.0 53 33956.5 54 31964.0 55 29171.5 56 26278.0 57 23229.5 58 21264.0 59 19354.5 60 16600.5 61 13399.0 62 10163.0 63 7419.5 64 5237.5 65 3843.5 66 2786.5 67 2101.0 68 1527.0 69 900.0 70 583.0 71 400.5 72 265.0 73 179.5 74 136.5 75 107.0 76 67.0 77 29.5 78 16.0 79 9.5 80 5.5 81 4.0 82 1.0 83 0.5 84 1.0 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026952904661741047 2 0.004816326606221768 3 2.778649965127943E-4 4 7.409733240341181E-4 5 9.262166550426477E-5 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.011346154024272434 12-13 0.0 14-15 0.0 16-17 0.0 18-19 6.02040825777721E-4 20-21 0.001435635815316104 22-23 0.002037676641093825 24-25 0.010836734863998977 26-27 0.005742543261264416 28-29 5.557299930255885E-4 30-31 0.002408163303110884 32-33 0.004677394107965371 34-35 0.0 36-37 0.007317111574836916 38-39 0.01477315564793023 40-41 0.0012967033170597068 42-43 0.0 44-45 0.0 46-47 4.1679749476919144E-4 48-49 0.0032880691254013996 50-51 0.006344584087042136 52-53 0.0 54-55 0.003149136627145002 56-57 0.0031028257943928697 58-59 2.315541637606619E-4 60-61 0.0015282574808203686 62-63 0.004955259104478165 64-65 0.0 66-67 0.0015282574808203686 68-69 3.241758292649267E-4 70-71 0.0011577708188033097 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.001204081651555442 80-81 0.0016208791463246332 82-83 0.009956829041708462 84-85 0.006715070749059196 86-87 0.0030565149616407373 88-89 9.262166550426477E-5 90-91 5.557299930255885E-4 92-93 4.6310832752132384E-5 94-95 0.0 96-97 0.0018524333100852952 98-99 0.0016208791463246332 100-101 4.6310832752132384E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1079661.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.746245712881255 #Duplication Level Percentage of deduplicated Percentage of total 1 81.43053345312173 50.28029727127451 2 10.953296283757249 13.526498474057282 3 3.2276613624312422 5.978879146879577 4 1.386916727748545 3.425476042194676 5 0.7536692745815209 2.3268124107279777 6 0.4650116783142881 1.7227635209131995 7 0.31271555437437143 1.3516308021047871 8 0.22973190143460148 1.1348065947254655 9 0.17225661534961575 0.9572579367341982 >10 0.9675719441798467 11.013718186573664 >50 0.06850636276277572 2.868216384605437 >100 0.03047736065115147 3.2531183465159588 >500 7.506733150481667E-4 0.26735406168743575 >1k 6.005386520385334E-4 0.676450273277815 >5k 3.002693260192667E-4 1.2167205477280145 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7291 0.6753045631915944 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5834 0.5403547965518807 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3838 0.35548195220536816 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1446 0.13393092831916686 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 9.262166550426477E-5 0.0 0.0 0.0 0.0 8 9.262166550426477E-5 0.0 0.0 0.0 0.0 9 9.262166550426477E-5 0.0 0.0 9.262166550426477E-5 0.0 10-11 9.262166550426477E-5 0.0 0.0 9.262166550426477E-5 0.0 12-13 9.262166550426477E-5 0.0 0.0 9.262166550426477E-5 0.0 14-15 9.262166550426477E-5 0.0 0.0 9.262166550426477E-5 0.0 16-17 1.3893249825639716E-4 0.0 0.0 2.315541637606619E-4 0.0 18-19 1.8524333100852953E-4 0.0 0.0 6.483516585298534E-4 0.0 20-21 1.8524333100852953E-4 0.0 0.0 7.872841567862505E-4 0.0 22-23 1.8524333100852953E-4 0.0 0.0 0.0010651491532990447 0.0 24-25 1.8524333100852953E-4 0.0 0.0 0.0011577708188033095 0.0 26-27 1.8524333100852953E-4 0.0 0.0 0.0014819466480682363 0.0 28-29 1.8524333100852953E-4 0.0 0.0 0.002408163303110884 0.0 30-31 3.7048666201705907E-4 0.0 0.0 0.004492150776956841 0.0 32-33 3.7048666201705907E-4 0.0 0.0 0.006715070749059195 0.0 34-35 3.7048666201705907E-4 0.0 0.0 0.009169544884922212 0.0 36-37 3.7048666201705907E-4 0.0 0.0 0.011624019020785227 4.6310832752132384E-5 38-39 3.7048666201705907E-4 0.0 0.0 0.013893249825639714 9.262166550426477E-5 40-41 3.7048666201705907E-4 0.0 0.0 0.017968603107827365 9.262166550426477E-5 42-43 3.7048666201705907E-4 0.0 0.0 0.02690659382898891 9.262166550426477E-5 44-45 3.7048666201705907E-4 0.0 0.0 0.04042935699261157 9.262166550426477E-5 46-47 3.7048666201705907E-4 0.0 0.0 0.054693093480268344 9.262166550426477E-5 48-49 3.7048666201705907E-4 0.0 0.0 0.08474882393640226 9.262166550426477E-5 50-51 3.7048666201705907E-4 0.0 0.0 0.11475824355978405 9.262166550426477E-5 52-53 3.7048666201705907E-4 0.0 0.0 0.14810204314131936 9.262166550426477E-5 54-55 3.7048666201705907E-4 0.0 0.0 0.21330769565632174 9.262166550426477E-5 56-57 3.7048666201705907E-4 0.0 0.0 0.27883752400058903 9.262166550426477E-5 58-59 3.7048666201705907E-4 0.0 0.0 0.35052669310089 9.262166550426477E-5 60-61 3.7048666201705907E-4 0.0 0.0 0.4337009487237198 9.262166550426477E-5 62-63 4.631083275213238E-4 0.0 0.0 0.5388728499038125 9.262166550426477E-5 64-65 4.631083275213238E-4 0.0 0.0 0.6089411398577886 9.262166550426477E-5 66-67 4.631083275213238E-4 0.0 0.0 0.7227731667625301 9.262166550426477E-5 68-69 4.631083275213238E-4 0.0 0.0 0.8914835304785484 9.262166550426477E-5 70-71 5.094191602734562E-4 0.0 0.0 1.00401885406623 9.262166550426477E-5 72-73 6.02040825777721E-4 0.0 0.0 1.1726829069494962 9.262166550426477E-5 74-75 6.483516585298534E-4 0.0 0.0 1.37279201527146 9.262166550426477E-5 76-77 6.483516585298534E-4 0.0 0.0 1.5281648591548644 9.262166550426477E-5 78-79 6.483516585298534E-4 0.0 0.0 1.674970198979124 9.262166550426477E-5 80-81 7.409733240341181E-4 0.0 0.0 1.8878610971406764 9.262166550426477E-5 82-83 7.409733240341181E-4 0.0 0.0 2.1379395940021917 9.262166550426477E-5 84-85 8.335949895383829E-4 0.0 0.0 2.4352551402708817 9.262166550426477E-5 86-87 9.262166550426476E-4 0.0 0.0 2.8163006721554265 9.262166550426477E-5 88-89 9.262166550426476E-4 0.0 0.0 3.2203163770850294 9.262166550426477E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGGCG 35 2.786995E-4 54.289154 5 GGTATCA 3840 0.0 47.13408 1 GTATCAA 6295 0.0 37.657032 1 TATCAAC 8185 0.0 28.668785 2 ATCAACG 8250 0.0 28.441593 3 TCAACGC 8400 0.0 27.990252 4 CAACGCA 8445 0.0 27.787434 5 AACGCAG 8710 0.0 27.051083 6 ACGCAGA 9850 0.0 23.727392 7 CGCAGAG 9870 0.0 23.534927 8 GTACATG 5025 0.0 23.25628 1 GAGTACT 6265 0.0 23.083776 12-13 TACATGG 4985 0.0 23.05957 2 ACATGGG 4860 0.0 22.381033 3 GCAGAGT 10370 0.0 22.125317 9 GTACTTT 6660 0.0 21.466095 14-15 AGTACTT 6420 0.0 21.453772 12-13 AGAGTAC 8860 0.0 21.365631 10-11 ACACCGT 185 6.5479E-6 20.541842 6 CATGGGG 1885 0.0 19.90658 4 >>END_MODULE