FastQCFastQC Report
Thu 26 May 2016
SRR936844_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936844_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123483
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58880.5240844765786399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42050.37428247690441246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27360.2435283844971397No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA61900.037.4784971
GGTATCA37550.035.955171
ATCAACG75100.030.449313
TCAACGC76450.029.911624
TATCAAC77400.029.790182
CAACGCA76900.029.6734395
AACGCAG78900.028.9173896
ACGCAGA87400.025.9338117
CGCAGAG87700.025.6283668
GATCGTG1309.991165E-625.5866897
GTATAGG2551.8189894E-1124.2357331
GCAGAGT91600.023.7580099
GAGTACT44450.022.98453712-13
GTACATG53700.022.928651
TACATGG54000.022.3620832
ATTCGCG753.0217823E-522.17908580-81
GTATTAC1954.1877502E-721.9412251
AGAGTAC74450.021.92359510-11
AGTACTT46800.021.5765612-13
ACATGGG53400.021.188883
Produced by FastQC (version 0.11.5)