##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936844_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1123483 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.884536748664644 33.0 31.0 34.0 30.0 34.0 2 31.974636020304715 33.0 31.0 34.0 30.0 34.0 3 31.989026981271635 33.0 31.0 34.0 30.0 34.0 4 35.59192172912274 37.0 35.0 37.0 33.0 37.0 5 35.597223990038124 37.0 35.0 37.0 33.0 37.0 6 35.670080455155976 37.0 35.0 37.0 33.0 37.0 7 35.6118303525732 37.0 35.0 37.0 33.0 37.0 8 35.585196215697074 37.0 35.0 37.0 33.0 37.0 9 37.22719702923854 39.0 37.0 39.0 34.0 39.0 10-11 37.115162401211236 39.0 37.0 39.0 33.0 39.0 12-13 37.03352787714634 39.0 37.0 39.0 33.0 39.0 14-15 38.20258294963075 40.0 38.0 41.0 33.0 41.0 16-17 38.1442794417005 40.0 38.0 41.0 33.0 41.0 18-19 38.1157592059693 40.0 38.0 41.0 33.0 41.0 20-21 38.06768460225922 40.0 38.0 41.0 33.0 41.0 22-23 37.962311401240605 40.0 38.0 41.0 33.0 41.0 24-25 37.9268524757384 40.0 38.0 41.0 33.0 41.0 26-27 37.94305610320761 40.0 38.0 41.0 33.0 41.0 28-29 37.87095398862289 40.0 38.0 41.0 33.0 41.0 30-31 37.71288662133739 40.0 37.0 41.0 33.0 41.0 32-33 37.52786602022461 40.0 37.0 41.0 32.5 41.0 34-35 37.35820835740283 40.0 37.0 41.0 32.0 41.0 36-37 37.195031878542 40.0 36.0 41.0 31.5 41.0 38-39 37.04298329391722 39.0 36.0 41.0 31.0 41.0 40-41 36.85000885638679 39.0 36.0 41.0 31.0 41.0 42-43 36.632094121584394 39.0 36.0 41.0 30.0 41.0 44-45 36.3723104844488 39.0 35.0 41.0 30.0 41.0 46-47 35.99992033702334 39.0 35.0 40.5 29.5 41.0 48-49 35.74496676852253 39.0 35.0 40.5 28.0 41.0 50-51 35.050134714989014 38.0 34.0 40.0 27.0 40.5 52-53 35.23550067068216 38.5 35.0 40.0 27.5 40.5 54-55 35.62452480366859 39.0 35.0 40.5 28.0 41.0 56-57 35.51324230095159 39.0 35.0 41.0 27.5 41.0 58-59 35.30598860863938 39.0 35.0 41.0 27.0 41.0 60-61 35.03450074455955 38.0 35.0 40.0 26.0 41.0 62-63 34.74183543498211 38.0 34.0 40.0 26.0 41.0 64-65 34.421990363895134 37.5 34.0 40.0 26.0 41.0 66-67 34.06318520173425 37.0 34.0 40.0 25.5 41.0 68-69 33.67349439199347 36.5 34.0 39.0 24.5 41.0 70-71 33.25806264981313 36.0 33.0 39.0 23.5 41.0 72-73 32.89010158587179 35.5 33.0 39.0 22.5 40.0 74-75 32.50283003837174 35.0 32.5 37.5 22.0 40.0 76-77 32.054359077974475 35.0 32.0 37.0 20.5 39.0 78-79 31.60068287637641 35.0 32.0 36.5 19.5 39.0 80-81 31.161401196101764 35.0 31.0 36.0 18.0 38.5 82-83 30.76869476440676 35.0 31.0 36.0 15.0 37.0 84-85 30.402759543313074 34.5 31.0 35.0 9.5 37.0 86-87 30.055477474959567 34.0 30.5 35.0 7.0 36.5 88-89 29.7059158883579 34.0 30.0 35.0 4.0 36.0 90-91 29.345533933312744 34.0 30.0 35.0 2.0 36.0 92-93 28.974664948201266 34.0 29.0 35.0 2.0 35.0 94-95 28.60980495477012 34.0 29.0 35.0 2.0 35.0 96-97 28.137195667402178 34.0 29.0 35.0 2.0 35.0 98-99 27.596211513658865 34.0 28.5 35.0 2.0 35.0 100-101 25.451983697127595 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.39603949911639447 1101 2 0.3109792381662757 1101 3 0.27484205103460013 1101 4 0.28495510842142835 1101 5 0.22453386262509412 1101 6 0.21294129599339584 1101 7 0.08915437495948453 1101 8 0.25988899155915135 1101 9 0.29035722748432846 1101 10-11 0.2034784401162142 1101 12-13 0.3489761474196982 1101 14-15 0.38911201857869315 1101 16-17 0.40013383763528765 1101 18-19 0.36085011982167003 1101 20-21 0.34281213060837246 1101 22-23 0.380181783709169 1101 24-25 0.3169869641322336 1101 26-27 0.384921381293033 1101 28-29 0.5228087938532795 1101 30-31 0.5278984091402421 1101 32-33 0.2790738523076328 1101 34-35 0.3379129077987386 1101 36-37 0.48050784034208505 1101 38-39 0.4617278299984804 1101 40-41 0.4204654980408975 1101 42-43 0.45268183373795523 1101 44-45 0.6706244967330193 1101 46-47 0.5386101150845448 1101 48-49 0.5106179856086115 1101 50-51 0.7525430295426858 1101 52-53 0.6932454566327948 1101 54-55 0.7700006142368068 1101 56-57 0.6737338514372482 1101 58-59 0.6623363527704953 1101 60-61 0.5830202775359155 1101 62-63 0.49875263734282527 1101 64-65 0.3632882403119666 1101 66-67 0.399711749101904 1101 68-69 0.37988782802887044 1101 70-71 0.3763337984561659 1101 72-73 0.3068275291746687 1101 74-75 0.43739978654721057 1101 76-77 0.5146113992225629 1101 78-79 0.35626894516378727 1101 80-81 0.3898152314055814 1101 82-83 0.31650189292676245 1101 84-85 0.3045056379274058 1101 86-87 0.3727094534575208 1101 88-89 0.29890382326876264 1101 90-91 0.581472445377976 1101 92-93 0.4647406805732537 1101 94-95 0.5732084834026914 1101 96-97 0.5255292218451437 1101 98-99 0.41530048853906365 1101 100-101 0.7399828637427994 1102 1 0.1423928899710596 1102 2 0.19106692541508608 1102 3 0.10348655231337389 1102 4 0.09183518486672426 1102 5 0.1938545674956771 1102 6 0.18417313320988882 1102 7 0.0998229902316794 1102 8 0.0288387119155189 1102 9 0.21927996714875775 1102 10-11 0.2628415833668214 1102 12-13 0.13523004697283625 1102 14-15 0.013088298053695269 1102 16-17 0.21735932314722106 1102 18-19 0.062480203293183934 1102 20-21 0.05712032880403939 1102 22-23 0.031046008997947183 1102 24-25 -0.21172217838765306 1102 26-27 -0.0981875813555817 1102 28-29 -0.12199601770345936 1102 30-31 -0.18134247785000923 1102 32-33 -0.016676688711449117 1102 34-35 0.003065657861981208 1102 36-37 0.09603298016389772 1102 38-39 0.014196844360078842 1102 40-41 -0.08301950800164803 1102 42-43 0.13190670094236623 1102 44-45 0.20799556556188747 1102 46-47 0.07092792515171453 1102 48-49 0.12741346634270911 1102 50-51 0.03422426390934419 1102 52-53 0.13812053040812344 1102 54-55 -0.08114184943762837 1102 56-57 0.3245587298836057 1102 58-59 0.1076695362110982 1102 60-61 0.08491735743810835 1102 62-63 -0.09228471405258887 1102 64-65 0.023469235787075604 1102 66-67 -0.19569591229041094 1102 68-69 0.0456279702396003 1102 70-71 -0.1312404687088886 1102 72-73 -0.14524520627402637 1102 74-75 -1.906059031142604E-5 1102 76-77 -0.035696349294905616 1102 78-79 -0.06512168466674595 1102 80-81 0.053776334241366186 1102 82-83 -0.04770653291849314 1102 84-85 -0.14959064941696454 1102 86-87 -0.11600362374660378 1102 88-89 0.0373906561289985 1102 90-91 0.051675643714165886 1102 92-93 0.07750029947098014 1102 94-95 0.2776434346407122 1102 96-97 0.08320607184935724 1102 98-99 0.31962302022732914 1102 100-101 0.1597209261918593 1103 1 0.17517283919941562 1103 2 0.22661289972481313 1103 3 0.2119449119503578 1103 4 0.1806036366000825 1103 5 0.16423016128666745 1103 6 0.26636723850509014 1103 7 0.30173968711957144 1103 8 0.2547882178545464 1103 9 0.26372754564557965 1103 10-11 0.35542775861407705 1103 12-13 0.19723234826271607 1103 14-15 0.42383115801080606 1103 16-17 0.39124393629322896 1103 18-19 0.42599737118437275 1103 20-21 0.24021211624365435 1103 22-23 0.26324257676287743 1103 24-25 0.2726265879150205 1103 26-27 0.3373897334778988 1103 28-29 0.38714937098620084 1103 30-31 0.34271920925516497 1103 32-33 0.2754530341935464 1103 34-35 0.3713978495048025 1103 36-37 0.33680655383427904 1103 38-39 0.2926397128238989 1103 40-41 0.33813445008264154 1103 42-43 0.44361843080496044 1103 44-45 0.3231267704259366 1103 46-47 0.1897300007898295 1103 48-49 0.33376686208845996 1103 50-51 0.25142976372353587 1103 52-53 0.3565290586123524 1103 54-55 0.32346095419607934 1103 56-57 0.17547297773342052 1103 58-59 0.30769953356954005 1103 60-61 0.3931816296887618 1103 62-63 0.39972390011422476 1103 64-65 0.38616102859935353 1103 66-67 0.46045309846834215 1103 68-69 0.3613060672429995 1103 70-71 0.25601087781432597 1103 72-73 0.06169336182220775 1103 74-75 0.12443147287017808 1103 76-77 0.2249639059960522 1103 78-79 0.2896127491666931 1103 80-81 0.3714720893912862 1103 82-83 0.4326516892679102 1103 84-85 0.48674331520406255 1103 86-87 0.47226561821566904 1103 88-89 0.49734507805303707 1103 90-91 0.5522279085147268 1103 92-93 0.5500435382007041 1103 94-95 0.7942516736166816 1103 96-97 0.5317724667489117 1103 98-99 0.30647410349014237 1103 100-101 0.5486205604031724 1104 1 0.09211490552085522 1104 2 0.059463380120114095 1104 3 0.1098937989309654 1104 4 0.21474885678969713 1104 5 0.201457544541789 1104 6 0.1557982526986592 1104 7 0.14902082029156816 1104 8 0.2536533688834339 1104 9 0.18176831925115522 1104 10-11 0.35666235864125184 1104 12-13 0.0862253252101084 1104 14-15 0.005444147043967007 1104 16-17 0.2073253967643396 1104 18-19 0.15429645665512481 1104 20-21 0.27860904448939294 1104 22-23 0.018142666683395703 1104 24-25 0.012833636411379246 1104 26-27 -0.028521716497671434 1104 28-29 0.052806238918691406 1104 30-31 0.08431988120140943 1104 32-33 -0.11363029697128724 1104 34-35 0.07834225780756299 1104 36-37 0.00990354944812566 1104 38-39 0.02487101383789536 1104 40-41 0.16905062739085963 1104 42-43 -0.15937019757465265 1104 44-45 -0.10795970651133047 1104 46-47 -0.31318157351314113 1104 48-49 -0.18039851070582102 1104 50-51 -0.25085005389104964 1104 52-53 -0.08862665423182392 1104 54-55 0.008144251821427417 1104 56-57 0.05255293181902232 1104 58-59 -0.02872319116669786 1104 60-61 -0.07889602578554644 1104 62-63 -0.1459548552238985 1104 64-65 -0.26139535966066774 1104 66-67 -0.4045642061713792 1104 68-69 -0.2732732868905998 1104 70-71 -0.20131084970048363 1104 72-73 -0.2610699737374489 1104 74-75 -0.36144146452374315 1104 76-77 -0.12518470862080733 1104 78-79 -0.11359981310543432 1104 80-81 -0.11751915275512559 1104 82-83 -0.1035384275709319 1104 84-85 -0.12123069747732984 1104 86-87 0.09002246272834924 1104 88-89 0.03251093948291839 1104 90-91 -0.04914590929069362 1104 92-93 -0.14471895308914995 1104 94-95 0.0765254772545596 1104 96-97 0.07655996808615484 1104 98-99 0.13869065869584674 1104 100-101 0.08139239648761531 1105 1 0.07168440050008229 1105 2 0.1614510372723572 1105 3 0.2513248299318498 1105 4 0.15243390537725077 1105 5 0.16507877497014078 1105 6 0.08507047193538142 1105 7 0.13041413133064594 1105 8 0.20274910524987888 1105 9 0.1703821619979351 1105 10-11 0.2215838780734245 1105 12-13 0.2335858976955265 1105 14-15 0.2780732717377674 1105 16-17 0.2701181229500449 1105 18-19 0.12512138970961217 1105 20-21 0.312363903530148 1105 22-23 0.28864476661048144 1105 24-25 0.2453097134440796 1105 26-27 0.3438365455545167 1105 28-29 0.12961102955920722 1105 30-31 0.2896728982849197 1105 32-33 0.24476610263211995 1105 34-35 -0.033247673951940726 1105 36-37 0.035226753385785514 1105 38-39 0.31294438635161015 1105 40-41 0.10757884148873131 1105 42-43 0.23965360159906623 1105 44-45 0.0759850073885815 1105 46-47 -0.08854898944475309 1105 48-49 0.23401391474705946 1105 50-51 -0.036957276248287485 1105 52-53 0.03387765001971843 1105 54-55 0.11065304002487153 1105 56-57 0.3863269588984366 1105 58-59 0.34645094704170987 1105 60-61 0.3019614929346659 1105 62-63 0.051283121680896215 1105 64-65 0.147667699763673 1105 66-67 0.17299104741150018 1105 68-69 0.1574955796507922 1105 70-71 0.07198316633449053 1105 72-73 0.17981773464366313 1105 74-75 0.07801548668560798 1105 76-77 0.1991342270053913 1105 78-79 0.3233615849747977 1105 80-81 0.17622305309820163 1105 82-83 0.20519192946801823 1105 84-85 0.3259448121585997 1105 86-87 0.2882750335844868 1105 88-89 0.3753892144507134 1105 90-91 0.23570455148385605 1105 92-93 0.3435434756154443 1105 94-95 0.35241459471470193 1105 96-97 0.19165234548201582 1105 98-99 0.1356577159113037 1105 100-101 0.13415869816287795 1106 1 0.21069737359762897 1106 2 0.19680718015152365 1106 3 0.2206160459670663 1106 4 0.25835941893622305 1106 5 0.12504983279612247 1106 6 0.1010051290663938 1106 7 0.20492750922954173 1106 8 0.35168382673008836 1106 9 0.24896974200419209 1106 10-11 0.3158663022124557 1106 12-13 0.3508174156332444 1106 14-15 0.2612725193011016 1106 16-17 0.2382798609171246 1106 18-19 0.374819098129926 1106 20-21 0.14499650403087827 1106 22-23 0.2644068182424846 1106 24-25 0.20615958676594204 1106 26-27 0.20360056357827716 1106 28-29 0.035850881466267026 1106 30-31 0.2670975536051685 1106 32-33 0.16757961416783473 1106 34-35 0.2539592566062936 1106 36-37 0.1560457390927752 1106 38-39 0.2146023509116617 1106 40-41 0.23232499886890423 1106 42-43 0.4133358273031291 1106 44-45 0.2737413154805637 1106 46-47 0.2737336262453809 1106 48-49 0.17443927790848335 1106 50-51 0.051398287488090943 1106 52-53 0.10820621103697192 1106 54-55 0.2911835916770684 1106 56-57 0.218256955950352 1106 58-59 0.3695579208485853 1106 60-61 0.32846288691786896 1106 62-63 0.46913089542312747 1106 64-65 0.34653889511736224 1106 66-67 0.0941692662556406 1106 68-69 0.23038862978779662 1106 70-71 0.2812534913278668 1106 72-73 0.21969553102783834 1106 74-75 0.4188537322571122 1106 76-77 0.40473777010214107 1106 78-79 0.37051046657077435 1106 80-81 0.3492737587694599 1106 82-83 0.31882812304835895 1106 84-85 0.3279950075257574 1106 86-87 0.41154303003480663 1106 88-89 0.49910092106625825 1106 90-91 0.37106795235391843 1106 92-93 0.5610744417961975 1106 94-95 0.8365113639360295 1106 96-97 0.8603284280824433 1106 98-99 0.6397420193462011 1106 100-101 0.930258538187779 1107 1 0.20692418877399987 1107 2 0.18926378307633485 1107 3 0.28705402275524605 1107 4 0.3468994599775357 1107 5 0.30896088551271106 1107 6 0.3284673352895737 1107 7 0.3082610506429191 1107 8 0.3259641422587052 1107 9 0.27629758796947357 1107 10-11 0.28169807246830914 1107 12-13 0.36738058724874634 1107 14-15 0.29400053787373537 1107 16-17 0.40489103957167316 1107 18-19 0.5155753402835757 1107 20-21 0.5825229475366527 1107 22-23 0.47932130229811065 1107 24-25 0.6392167681532825 1107 26-27 0.62674372165867 1107 28-29 0.6004176241316941 1107 30-31 0.5971496627936972 1107 32-33 0.46334310444306226 1107 34-35 0.45296651661816156 1107 36-37 0.44537162910562955 1107 38-39 0.6464695126106648 1107 40-41 0.6547299682167349 1107 42-43 0.7032076797208759 1107 44-45 0.6085862017152763 1107 46-47 0.5360309092154196 1107 48-49 0.5411065390895757 1107 50-51 0.23644783531033653 1107 52-53 0.2665244090241501 1107 54-55 0.3404856812961583 1107 56-57 0.5224709732130606 1107 58-59 0.7287506495409488 1107 60-61 0.7762897881006481 1107 62-63 0.7581783545180585 1107 64-65 0.8271983503241671 1107 66-67 0.7196757182233284 1107 68-69 0.8233905015173448 1107 70-71 0.6389624111249006 1107 72-73 0.7551657473419056 1107 74-75 0.737200843462027 1107 76-77 0.735154019370146 1107 78-79 0.6143099289315721 1107 80-81 0.6608092210589156 1107 82-83 0.8149479256299408 1107 84-85 0.6700895016286701 1107 86-87 0.7127919066502777 1107 88-89 0.7054633152550807 1107 90-91 0.9590159561231388 1107 92-93 0.9688355302331892 1107 94-95 1.0021851915288345 1107 96-97 0.8552324486843474 1107 98-99 1.2038737756309636 1107 100-101 1.272733521516603 1108 1 0.09916715902013351 1108 2 0.21153231957732999 1108 3 0.13391640750902312 1108 4 0.06384932654216868 1108 5 0.0494025266227851 1108 6 0.058371350718218196 1108 7 0.047695887612150045 1108 8 0.11681459222865698 1108 9 0.11294023433772793 1108 10-11 -0.12444014763337918 1108 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-2.0471374525473536 2316 62-63 -1.8860656716750412 2316 64-65 -2.3760439092956886 2316 66-67 -1.9426555386116249 2316 68-69 -1.8512886152845809 2316 70-71 -1.6197732714409234 2316 72-73 -1.932984929401755 2316 74-75 -2.0729979070969478 2316 76-77 -2.348615644272538 2316 78-79 -2.9189565491235108 2316 80-81 -2.705200847080338 2316 82-83 -3.0446626313055205 2316 84-85 -2.8350654387992904 2316 86-87 -3.3248502715035464 2316 88-89 -3.1973848966982885 2316 90-91 -3.571298263684618 2316 92-93 -3.6337439323566336 2316 94-95 -3.9405508275271366 2316 96-97 -4.524354996691347 2316 98-99 -5.087794108598718 2316 100-101 -5.240720771167833 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1853.0 3 676.0 4 596.0 5 664.0 6 757.0 7 844.0 8 1043.0 9 1367.0 10 1844.0 11 2434.0 12 3257.0 13 4089.0 14 4709.0 15 6135.0 16 8109.0 17 11184.0 18 10047.0 19 5776.0 20 5473.0 21 5761.0 22 6261.0 23 6746.0 24 7777.0 25 9049.0 26 10629.0 27 12710.0 28 15109.0 29 18587.0 30 23080.0 31 29265.0 32 37201.0 33 49879.0 34 68802.0 35 99810.0 36 152936.0 37 233056.0 38 224386.0 39 41560.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.491312369988314 19.4957861966774 14.951877867380958 22.061023565953324 2 14.811876568734034 20.859944493860404 41.242023329673884 23.086155607731683 3 18.900269599376042 25.870054525316345 30.226470598710055 25.003205276597562 4 12.453445189558899 17.38936831512107 38.223748074131464 31.93343842118856 5 13.340568630176532 37.83668188573934 34.1434727286336 14.679276755450532 6 30.321145892755275 37.1374073017684 18.118136765544783 14.423310039931547 7 27.138299123160188 33.788974122358525 21.44837120145938 17.624355553021907 8 25.624648022868712 35.15431746733296 20.2450117266305 18.976022783167828 9 26.16876469503378 17.376111788724728 21.121914321326653 35.333209194914836 10-11 24.186545226628724 27.58393809088313 27.658687326797153 20.570829355690986 12-13 25.52871241260883 25.10870025190039 28.18629492613372 21.176292409357064 14-15 22.52020599289305 26.081342427997363 26.98080196835278 24.41764961075681 16-17 21.31897475818781 29.537493971117478 27.331646656491976 21.811884614202732 18-19 21.17717900685264 27.724732837890926 30.262383165290885 20.835704989965553 20-21 22.18458031850484 26.850993729928668 30.2087930635972 20.75563288796929 22-23 21.84780377736847 26.900738670944378 30.05657459881989 21.194882952867264 24-25 21.601525066975384 27.042075985402725 30.19556055246735 21.16083839515454 26-27 21.388455572937644 27.270421049586428 30.017602146972365 21.323521230503566 28-29 21.250555792189132 27.40946762785891 30.20460718900228 21.13536939094968 30-31 21.444552695868442 27.16190432107145 30.077482601019128 21.316060382040984 32-33 21.219568970620372 27.281940535731213 30.022552465527042 21.475938028121373 34-35 21.57677068803293 27.399632007021594 29.84334928800391 21.180248016941565 36-37 21.628015141635714 27.16361378592077 29.82092189506339 21.387449177380123 38-39 21.452480996804873 27.43621206315127 29.816334714372374 21.294972225671483 40-41 21.674049828665837 27.290356550421734 29.818731615348014 21.216862005564412 42-43 21.497340292959432 27.390086805431928 29.54055074094382 21.57202216066482 44-45 21.590009368442367 27.552967303913604 29.345477445641894 21.51154588200213 46-47 21.748739719821035 27.813705955839126 29.008719042636567 21.42883528170327 48-49 21.827278557374658 27.869978935975244 28.673331378098133 21.62941112855197 50-51 21.649532712360077 28.13214455630706 28.54911849777227 21.669204233560592 52-53 21.998487632905157 28.23212832065387 28.250242900041794 21.519141146399182 54-55 21.62439815385629 28.071034425354608 28.376736880010007 21.927830540779098 56-57 21.705674781636453 28.369368214528606 28.245053215543425 21.67990378829151 58-59 21.96501460061607 27.989877262249852 28.2803318285419 21.764776308592175 60-61 21.716416914045634 27.946539438957846 28.737766423812474 21.599277223184046 62-63 21.653842781407022 28.067061345900402 28.813349600016736 21.465746272675844 64-65 21.51299931282734 28.128282317587704 28.61143946649766 21.74727890308729 66-67 21.595304497630657 28.31003314184219 28.249224872629824 21.84543748789733 68-69 21.515814578123553 28.187049821531623 28.495228375403425 21.8019072249414 70-71 21.663560350490442 28.11694890722246 28.408430475299777 21.81106026698732 72-73 21.615667368831453 28.183719420115438 28.242690580325174 21.957922630727936 74-75 21.550894541414547 28.451241995774996 28.042050612785573 21.95581285002488 76-77 21.57556839782016 28.29965429161833 28.096395925573432 22.028381384988084 78-79 21.637402688982704 28.4392797504493 27.95020382615323 21.973113734414767 80-81 21.639056999697555 28.532498428763407 27.904671928917303 21.923772642621735 82-83 21.668762785396765 28.597885776739986 27.992200212357233 21.741151225506016 84-85 21.595843741487272 28.524380451483903 27.810913739475374 22.068862067553443 86-87 21.66239483480098 28.264656790921546 28.026956839583477 22.045991534693997 88-89 21.706912398072053 28.19485559984753 27.89993694627609 22.198295055804326 90-91 21.517870222648096 28.341981208737433 28.065335281238884 22.074813287375587 92-93 21.72352484648358 28.359717541368507 27.886622932687878 22.030134679460037 94-95 21.59740518135822 28.333090747879826 27.75115272601509 22.318351344746866 96-97 21.512565540812133 28.328409379742098 27.86938443383733 22.28964064560844 98-99 21.491479600196627 28.420493485028747 27.58995932150261 22.49806759327202 100-101 21.388384528622616 28.410089145553325 27.754460395889645 22.44706592993441 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 496.0 1 342.0 2 216.0 3 274.0 4 277.0 5 292.0 6 491.0 7 899.5 8 1482.0 9 1824.0 10 1710.0 11 1621.5 12 1681.5 13 1769.0 14 1893.0 15 1986.5 16 2068.0 17 2124.5 18 2250.5 19 2430.0 20 2640.5 21 3006.5 22 3518.5 23 4172.0 24 5004.0 25 6137.5 26 7827.5 27 9889.0 28 11544.0 29 13323.5 30 15773.5 31 18334.0 32 20462.5 33 22235.0 34 24573.0 35 26942.5 36 29682.5 37 32870.0 38 34707.0 39 35065.5 40 35416.0 41 36714.0 42 37704.5 43 38585.0 44 40272.0 45 41422.5 46 40874.0 47 40775.0 48 42794.5 49 42860.5 50 39992.0 51 37992.0 52 36363.0 53 34742.0 54 33157.5 55 30315.5 56 27109.5 57 24087.0 58 21882.5 59 19810.5 60 16795.0 61 13453.5 62 10302.5 63 7683.0 64 5651.5 65 3991.0 66 2734.0 67 2074.0 68 1510.5 69 935.5 70 619.0 71 401.0 72 256.0 73 192.5 74 142.5 75 94.5 76 63.5 77 34.0 78 23.0 79 17.5 80 13.0 81 8.5 82 4.5 83 5.0 84 5.0 85 3.5 86 1.5 87 1.5 88 1.5 89 1.0 90 0.5 91 1.0 92 1.5 93 1.0 94 0.0 95 0.5 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.045127518618439263 2 0.03239924413631537 3 0.02981798567490563 4 0.05456246333945418 5 0.10743375734212265 6 0.1391209301787388 7 0.07254226365685995 8 0.11135014949046848 9 0.09612962545939725 10-11 0.09466097840376757 12-13 0.09038855060557215 14-15 0.08188819946541247 16-17 0.06786929575258371 18-19 0.07565757559304413 20-21 0.06840334922735813 22-23 0.0402765328892382 24-25 0.010903591776644597 26-27 0.0036048609547273966 28-29 0.007699270927998021 30-31 0.006186119416137138 32-33 0.009123413527396498 34-35 0.008188819946541248 36-37 0.00805530657784764 38-39 0.00476197681673866 40-41 0.008322333315234855 42-43 0.004539454535582648 44-45 0.0027147718301033484 46-47 0.0020027005304041096 48-49 3.11531193618417E-4 50-51 0.0028037807425657533 52-53 0.006987199628298781 54-55 0.014998001749915219 56-57 0.011304131882725417 58-59 0.006364137241061947 60-61 0.0033823386735713844 62-63 0.010992600689107 64-65 0.0034713475860337893 66-67 0.024966999945704567 68-69 0.05296030291513089 70-71 0.06858136705228295 72-73 0.004672967904276255 74-75 0.015131515118608826 76-77 0.025100513314398172 78-79 0.030663570343298474 80-81 0.08540405150767746 82-83 0.033422846629633025 84-85 0.014686470556296802 86-87 0.03814031899014048 88-89 0.05616462376377746 90-91 0.050512557822414754 92-93 0.04962246869779072 94-95 0.014908992837452815 96-97 0.02069457214750913 98-99 0.04726373251753698 100-101 0.0933703491730627 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1123483.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.03773605694859 #Duplication Level Percentage of deduplicated Percentage of total 1 82.49680241157152 52.82908458374457 2 10.509969916017093 13.460693588967453 3 2.95071309642121 5.668709593452088 4 1.3018009078671218 3.3345753174676322 5 0.6859027661430245 2.196183014949897 6 0.41004090268251603 1.575485465912154 7 0.2962349843832237 1.3279152418537017 8 0.20686054806025272 1.0597504941822555 9 0.14733287059599678 0.8491377131765103 >10 0.9035615227902416 10.662819001687424 >50 0.06378947286775252 2.7693119679734357 >100 0.025877588384281978 2.7735727905346232 >500 4.1737958093599186E-4 0.16348653237184604 >1k 5.565061079146559E-4 0.8046922944863832 >5k 1.3912652697866396E-4 0.524582399240016 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5888 0.5240844765786399 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4205 0.37428247690441246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2736 0.2435283844971397 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.6702673738721456E-4 0.0 3 0.0 0.0 0.0 3.560356498496194E-4 0.0 4 0.0 0.0 0.0 3.560356498496194E-4 0.0 5 0.0 0.0 0.0 3.560356498496194E-4 0.0 6 0.0 0.0 0.0 3.560356498496194E-4 0.0 7 0.0 0.0 0.0 3.560356498496194E-4 0.0 8 0.0 0.0 0.0 3.560356498496194E-4 8.900891246240485E-5 9 0.0 0.0 0.0 3.560356498496194E-4 8.900891246240485E-5 10-11 0.0 0.0 0.0 3.560356498496194E-4 8.900891246240485E-5 12-13 0.0 0.0 0.0 4.0054010608082187E-4 8.900891246240485E-5 14-15 0.0 0.0 0.0 4.450445623120243E-4 1.3351336869360728E-4 16-17 0.0 0.0 0.0 4.450445623120243E-4 1.780178249248097E-4 18-19 0.0 0.0 0.0 4.895490185432268E-4 1.780178249248097E-4 20-21 0.0 0.0 0.0 6.675668434680364E-4 1.780178249248097E-4 22-23 0.0 4.450445623120243E-5 0.0 8.010802121616437E-4 1.780178249248097E-4 24-25 8.900891246240485E-5 8.900891246240485E-5 0.0 9.345935808552511E-4 1.780178249248097E-4 26-27 8.900891246240485E-5 8.900891246240485E-5 0.0 0.0014686470556296801 1.780178249248097E-4 28-29 8.900891246240485E-5 8.900891246240485E-5 0.0 0.0027147718301033484 1.780178249248097E-4 30-31 8.900891246240485E-5 8.900891246240485E-5 0.0 0.004539454535582648 1.780178249248097E-4 32-33 8.900891246240485E-5 8.900891246240485E-5 0.0 0.006987199628298781 1.780178249248097E-4 34-35 8.900891246240485E-5 8.900891246240485E-5 0.0 0.009835484827095738 1.780178249248097E-4 36-37 8.900891246240485E-5 8.900891246240485E-5 0.0 0.012372238832274274 1.780178249248097E-4 38-39 8.900891246240485E-5 8.900891246240485E-5 0.0 0.015131515118608826 1.780178249248097E-4 40-41 8.900891246240485E-5 8.900891246240485E-5 0.0 0.018780880529567424 1.780178249248097E-4 42-43 8.900891246240485E-5 8.900891246240485E-5 0.0 0.02817132079435114 1.780178249248097E-4 44-45 8.900891246240485E-5 8.900891246240485E-5 0.0 0.041344639838787056 1.780178249248097E-4 46-47 1.780178249248097E-4 8.900891246240485E-5 0.0 0.05509651681422861 1.780178249248097E-4 48-49 2.6702673738721456E-4 8.900891246240485E-5 0.0 0.08317882869611734 1.780178249248097E-4 50-51 2.6702673738721456E-4 8.900891246240485E-5 0.0 0.11077159155946285 1.780178249248097E-4 52-53 2.6702673738721456E-4 8.900891246240485E-5 0.0 0.14593011198211275 1.780178249248097E-4 54-55 2.6702673738721456E-4 8.900891246240485E-5 0.0 0.2109956269921307 1.780178249248097E-4 56-57 3.560356498496194E-4 8.900891246240485E-5 0.0 0.27672870884561673 1.780178249248097E-4 58-59 3.560356498496194E-4 8.900891246240485E-5 0.0 0.33992503669392415 1.780178249248097E-4 60-61 3.560356498496194E-4 8.900891246240485E-5 0.0 0.4234153965836599 1.780178249248097E-4 62-63 4.450445623120243E-4 1.780178249248097E-4 0.0 0.5259981681965815 1.780178249248097E-4 64-65 4.450445623120243E-4 1.780178249248097E-4 0.0 0.5956476421984134 1.780178249248097E-4 66-67 4.450445623120243E-4 1.780178249248097E-4 0.0 0.7097125635189852 1.780178249248097E-4 68-69 4.450445623120243E-4 1.780178249248097E-4 0.0 0.8730884223437292 1.780178249248097E-4 70-71 4.895490185432268E-4 1.780178249248097E-4 0.0 0.9827028980411809 1.780178249248097E-4 72-73 5.340534747744291E-4 1.780178249248097E-4 0.0 1.1405602042932559 3.560356498496194E-4 74-75 5.340534747744291E-4 1.780178249248097E-4 0.0 1.3368248562728586 3.560356498496194E-4 76-77 5.340534747744291E-4 1.780178249248097E-4 0.0 1.4834670395546705 3.560356498496194E-4 78-79 6.230623872368339E-4 1.780178249248097E-4 0.0 1.6176924795479772 3.560356498496194E-4 80-81 8.010802121616437E-4 1.780178249248097E-4 0.0 1.8333165699881528 4.895490185432268E-4 82-83 8.010802121616437E-4 1.780178249248097E-4 0.0 2.073818651461571 6.23062387236834E-4 84-85 8.010802121616437E-4 1.780178249248097E-4 0.0 2.360916898609058 6.23062387236834E-4 86-87 8.010802121616437E-4 1.780178249248097E-4 0.0 2.744901346971872 6.23062387236834E-4 88-89 8.010802121616437E-4 1.780178249248097E-4 0.0 3.1373416420186153 6.23062387236834E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6190 0.0 37.478497 1 GGTATCA 3755 0.0 35.95517 1 ATCAACG 7510 0.0 30.44931 3 TCAACGC 7645 0.0 29.91162 4 TATCAAC 7740 0.0 29.79018 2 CAACGCA 7690 0.0 29.673439 5 AACGCAG 7890 0.0 28.917389 6 ACGCAGA 8740 0.0 25.933811 7 CGCAGAG 8770 0.0 25.628366 8 GATCGTG 130 9.991165E-6 25.586689 7 GTATAGG 255 1.8189894E-11 24.235733 1 GCAGAGT 9160 0.0 23.758009 9 GAGTACT 4445 0.0 22.984537 12-13 GTACATG 5370 0.0 22.92865 1 TACATGG 5400 0.0 22.362083 2 ATTCGCG 75 3.0217823E-5 22.179085 80-81 GTATTAC 195 4.1877502E-7 21.941225 1 AGAGTAC 7445 0.0 21.923595 10-11 AGTACTT 4680 0.0 21.57656 12-13 ACATGGG 5340 0.0 21.18888 3 >>END_MODULE