FastQCFastQC Report
Thu 26 May 2016
SRR936844_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936844_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1123483
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73430.6535924442114389No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58750.5229273607166286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39070.3477578209906158No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15860.1411681351653741No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42850.042.4627721
GTATCAA68350.036.5601161
ATCAACG87650.028.3408873
TATCAAC87850.028.3316942
CAACGCA88950.027.9266875
TCAACGC89000.027.9114
AACGCAG91800.027.2197466
GTACATG49700.024.2793851
ACGCAGA103050.023.9254657
CGCAGAG103450.023.6503248
TACATGG50300.023.6077772
AGTACTT65100.022.10972412-13
GCAGAGT107850.022.0687629
GAGTACT63350.021.93314712-13
AGAGTAC90550.021.72062110-11
ACATGGG51300.021.6651763
GTACTTT65900.020.93794614-15
CAGAGTA104600.019.25726110-11
TACTTTT74600.019.13282214-15
CATGGGG20900.018.8623374
Produced by FastQC (version 0.11.5)