FastQCFastQC Report
Thu 26 May 2016
SRR936843_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936843_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576637
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74010.2872348724325545No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.21035171038838613No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34080.1322654297054649No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA137500.029.0062831
GGTATCA95700.028.2142941
GTACATG125050.024.7854441
ATCAACG161800.024.5002023
TCAACGC162950.024.3272954
TATCAAC164200.024.1453862
CAACGCA165350.023.9134465
TACATGG128150.023.7411982
AACGCAG169800.023.285386
ACATGGG129350.022.525593
GAGTACT70550.021.85761312-13
ACGCAGA187100.020.8779247
CGCAGAG188050.020.3953748
GTACTTT77600.019.87202814-15
AGTACTT74350.019.07868412-13
GCAGAGT196200.019.0402189
CATGGGA77150.019.0372714
CATGGGG57200.018.8630524
AGAGTAC156050.018.0281610-11
GTGTAGC12700.017.9667841
Produced by FastQC (version 0.11.5)