##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936843_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2576637 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.744274416613592 33.0 31.0 34.0 30.0 34.0 2 31.84006517021994 33.0 31.0 34.0 30.0 34.0 3 31.85956811145691 33.0 31.0 34.0 30.0 34.0 4 35.42918890010506 37.0 35.0 37.0 33.0 37.0 5 35.45481455090492 37.0 35.0 37.0 33.0 37.0 6 35.508385542860715 37.0 35.0 37.0 33.0 37.0 7 35.4472810877124 37.0 35.0 37.0 33.0 37.0 8 35.412957277257135 37.0 35.0 37.0 33.0 37.0 9 37.03153839675515 39.0 37.0 39.0 33.0 39.0 10-11 36.89557822852035 39.0 37.0 39.0 33.0 39.0 12-13 36.788238118136164 39.0 37.0 39.0 33.0 39.0 14-15 37.950123940624934 40.0 38.0 41.0 33.0 41.0 16-17 37.88347931819655 40.0 38.0 41.0 33.0 41.0 18-19 37.82775765464829 40.0 38.0 41.0 32.5 41.0 20-21 37.761785032195064 40.0 37.5 41.0 32.0 41.0 22-23 37.62611710535865 40.0 37.0 41.0 32.0 41.0 24-25 37.46010982532658 40.0 37.0 41.0 32.0 41.0 26-27 37.426257947859945 40.0 37.0 41.0 32.0 41.0 28-29 37.505452261998876 40.0 37.0 41.0 32.0 41.0 30-31 37.39402251849989 40.0 37.0 41.0 32.0 41.0 32-33 37.237577508977786 40.0 36.5 41.0 31.0 41.0 34-35 37.06428515153667 39.5 36.0 41.0 31.0 41.0 36-37 36.92987234911243 39.0 36.0 41.0 30.5 41.0 38-39 36.78276276402147 39.0 36.0 41.0 30.0 41.0 40-41 36.58733981542608 39.0 35.5 41.0 30.0 41.0 42-43 36.39846493704779 39.0 35.0 41.0 30.0 41.0 44-45 36.17539451618524 39.0 35.0 40.5 29.5 41.0 46-47 35.875769850390256 39.0 35.0 40.0 28.5 41.0 48-49 35.57988028581441 39.0 35.0 40.0 27.0 41.0 50-51 34.94023624592832 38.5 34.0 39.5 26.5 40.5 52-53 35.258034989018626 38.5 34.5 40.0 27.5 40.5 54-55 35.575638710458634 39.0 35.0 40.5 28.0 41.0 56-57 35.465957952167884 39.0 35.0 41.0 27.5 41.0 58-59 35.22503616147715 38.5 35.0 40.0 26.5 41.0 60-61 34.94141374978315 38.0 34.0 40.0 26.0 41.0 62-63 34.63127324493128 38.0 34.0 40.0 26.0 41.0 64-65 34.319107813789834 37.0 34.0 40.0 26.0 41.0 66-67 33.949462613476406 37.0 33.5 39.5 25.5 41.0 68-69 33.51541990586955 36.0 33.0 39.0 24.0 41.0 70-71 33.166697908940996 36.0 33.0 39.0 23.5 40.5 72-73 32.78414188727399 35.0 32.5 38.5 23.0 40.0 74-75 32.35286518822791 35.0 32.0 37.0 22.0 39.5 76-77 31.9061474317104 35.0 32.0 37.0 20.5 39.0 78-79 31.478153888188366 35.0 31.0 36.0 20.0 39.0 80-81 31.06804858425925 35.0 31.0 36.0 18.0 37.5 82-83 30.66610217116342 34.5 31.0 35.5 17.5 37.0 84-85 30.320117075086635 34.0 30.5 35.0 13.5 37.0 86-87 29.993557687792265 34.0 30.0 35.0 8.5 36.0 88-89 29.638493315123547 34.0 30.0 35.0 7.0 36.0 90-91 29.309674199353655 34.0 29.5 35.0 2.0 35.5 92-93 28.96614327124853 34.0 29.0 35.0 2.0 35.0 94-95 28.587459157032985 34.0 29.0 35.0 2.0 35.0 96-97 28.155339110631417 34.0 29.0 35.0 2.0 35.0 98-99 27.563878807919004 34.0 28.0 35.0 2.0 35.0 100-101 25.390674161707683 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5718124006088274 1101 2 0.4034206525927182 1101 3 0.47605172430852605 1101 4 0.35268560517798164 1101 5 0.43246881203306486 1101 6 0.4144790624941166 1101 7 0.4322553406974947 1101 8 0.39010454163205566 1101 9 0.47406817918970034 1101 10-11 0.4891374354333422 1101 12-13 0.5531417911174259 1101 14-15 0.8201043981831688 1101 16-17 0.8169369445577743 1101 18-19 0.7871325479343554 1101 20-21 0.7232787188895813 1101 22-23 0.8172068595258395 1101 24-25 0.7919334143862855 1101 26-27 0.8567588333533536 1101 28-29 0.8389700243107967 1101 30-31 0.7819319800954219 1101 32-33 0.812218848916757 1101 34-35 0.8143123810728454 1101 36-37 0.7332297645007273 1101 38-39 0.7798421665343511 1101 40-41 0.7913582966198405 1101 42-43 0.8188106556847785 1101 44-45 0.8025607526313223 1101 46-47 0.8419556550900467 1101 48-49 0.8899416542287 1101 50-51 0.9071198517053318 1101 52-53 1.044206852414213 1101 54-55 1.0195787624975452 1101 56-57 1.0380355925222773 1101 58-59 0.9889565396852547 1101 60-61 1.0549840005418218 1101 62-63 1.1414499158506928 1101 64-65 1.0159052500180294 1101 66-67 0.9302640975830698 1101 68-69 0.9407232429467953 1101 70-71 0.9477457493413937 1101 72-73 1.0355064278134165 1101 74-75 1.0050795246467885 1101 76-77 1.0357618215956812 1101 78-79 1.0344905463202956 1101 80-81 1.0986537939189596 1101 82-83 1.0210802795777845 1101 84-85 0.962518817676731 1101 86-87 1.1412115896305046 1101 88-89 1.1990495194424042 1101 90-91 1.2794094722048328 1101 92-93 1.071523275774691 1101 94-95 1.2749554600757769 1101 96-97 1.289881173251402 1101 98-99 1.3146902847729827 1101 100-101 1.4882608075420158 1102 1 0.4528864485284032 1102 2 0.4328463183122615 1102 3 0.3599587988124 1102 4 0.28494040519799313 1102 5 0.37643938636734475 1102 6 0.1812756573018106 1102 7 0.2765195044403157 1102 8 0.16955383907482968 1102 9 0.2760593603866326 1102 10-11 0.35997477689228674 1102 12-13 0.2691454721058477 1102 14-15 0.4896050181683762 1102 16-17 0.34313340480365895 1102 18-19 0.41975933907996676 1102 20-21 0.3688056507134476 1102 22-23 0.5178610412287625 1102 24-25 0.48951825227266 1102 26-27 0.46781959190386857 1102 28-29 0.5086791595009359 1102 30-31 0.3795198762048031 1102 32-33 0.4944945004572645 1102 34-35 0.4139048033522741 1102 36-37 0.4575309605439415 1102 38-39 0.35096253263145627 1102 40-41 0.3576355495571235 1102 42-43 0.543733227038679 1102 44-45 0.35558894944743713 1102 46-47 0.2685305009278025 1102 48-49 0.21892721385571434 1102 50-51 0.187654818315103 1102 52-53 0.30824409601002145 1102 54-55 0.28982836909886345 1102 56-57 0.4332747733633511 1102 58-59 0.2941074212875421 1102 60-61 0.2859034691894706 1102 62-63 0.4579468048038322 1102 64-65 0.5242722701411324 1102 66-67 0.2994805808490284 1102 68-69 0.2924995144725173 1102 70-71 0.39743520069619365 1102 72-73 0.4175382555742999 1102 74-75 0.3655620010289695 1102 76-77 0.3475281955996756 1102 78-79 0.38646838254411264 1102 80-81 0.43214884237789164 1102 82-83 0.3745177222361029 1102 84-85 0.4572684048166238 1102 86-87 0.592223027384545 1102 88-89 0.47469410944590607 1102 90-91 0.410694815532068 1102 92-93 0.3928646679890129 1102 94-95 0.6014110352163158 1102 96-97 0.4607385321700157 1102 98-99 0.5299308001113587 1102 100-101 1.0397626730580463 1103 1 0.2584689306266412 1103 2 0.41591135487163555 1103 3 0.3903253139777405 1103 4 0.3022093280329017 1103 5 0.19910122643241124 1103 6 0.19851883304183815 1103 7 0.23083873770086427 1103 8 0.21821630008152937 1103 9 0.2405280683311588 1103 10-11 0.3751707574545833 1103 12-13 0.2707544396834791 1103 14-15 0.3919681196604117 1103 16-17 0.5445983994449435 1103 18-19 0.5619941959486994 1103 20-21 0.4086667458894695 1103 22-23 0.33696842709159824 1103 24-25 0.640917569236862 1103 26-27 0.6575809509650199 1103 28-29 0.5209855932934104 1103 30-31 0.5877606479792448 1103 32-33 0.5022446771485889 1103 34-35 0.4313285851759119 1103 36-37 0.3671698748537011 1103 38-39 0.41348272753846516 1103 40-41 0.6000026651868708 1103 42-43 0.5669361024019608 1103 44-45 0.5371374369486546 1103 46-47 0.477857845276084 1103 48-49 0.4403078122302375 1103 50-51 0.44187673960247054 1103 52-53 0.44446971644725153 1103 54-55 0.3952375024817556 1103 56-57 0.4497451598377822 1103 58-59 0.46582330405303907 1103 60-61 0.44728558227613746 1103 62-63 0.43374656829192304 1103 64-65 0.5013380830281164 1103 66-67 0.5329981489500923 1103 68-69 0.5462099569220342 1103 70-71 0.4444180025402247 1103 72-73 0.36356383764685773 1103 74-75 0.39949343485908173 1103 76-77 0.30067435508169993 1103 78-79 0.2931164932343364 1103 80-81 0.26078077124484267 1103 82-83 0.2816660944626044 1103 84-85 0.42826196864518096 1103 86-87 0.4730215818131498 1103 88-89 0.463618266333949 1103 90-91 0.4293351852302365 1103 92-93 0.5949536909033206 1103 94-95 0.5961090157707822 1103 96-97 0.37830905737762777 1103 98-99 0.6745124068083754 1103 100-101 0.9260543927622216 1104 1 0.5976503634702723 1104 2 0.5448056624873736 1104 3 0.570567032498289 1104 4 0.5431633165464831 1104 5 0.4706689735382099 1104 6 0.4830761637278371 1104 7 0.5198300908523379 1104 8 0.5426240949727728 1104 9 0.5705557442152198 1104 10-11 0.6765897060869861 1104 12-13 0.6881564527463553 1104 14-15 0.8871527089416915 1104 16-17 0.7173154901152898 1104 18-19 0.8290724020458242 1104 20-21 0.7699842777237578 1104 22-23 0.7288736040531134 1104 24-25 0.7616581119931638 1104 26-27 0.802986868320076 1104 28-29 0.8394471986000198 1104 30-31 0.8148755972576822 1104 32-33 0.8195711147716409 1104 34-35 0.8349135030918475 1104 36-37 0.7654052629436734 1104 38-39 0.8758185528424605 1104 40-41 0.8225313000275918 1104 42-43 0.9026804327078892 1104 44-45 0.8923266432660881 1104 46-47 0.9597114009644727 1104 48-49 0.8901409932716078 1104 50-51 0.9495455401122754 1104 52-53 1.027013720927357 1104 54-55 0.9949042595580693 1104 56-57 1.0205299857641066 1104 58-59 0.9728728423933077 1104 60-61 1.0540262356306371 1104 62-63 0.9874092999446944 1104 64-65 0.9786070798058475 1104 66-67 0.9547472858891055 1104 68-69 0.8801478498857946 1104 70-71 0.9493178979985899 1104 72-73 0.9089483814599859 1104 74-75 0.8444074096444538 1104 76-77 0.8041533708278941 1104 78-79 0.8694985977426164 1104 80-81 1.071190212820465 1104 82-83 1.038773784636657 1104 84-85 0.9939024419703841 1104 86-87 0.9406349603337496 1104 88-89 0.9682636103689752 1104 90-91 1.0799894701645663 1104 92-93 1.0421577507351962 1104 94-95 0.9514744331166192 1104 96-97 0.8755179887970854 1104 98-99 0.94346902685702 1104 100-101 1.1934345495132348 1105 1 0.31974675633355787 1105 2 0.2470609449503307 1105 3 0.30242108207932006 1105 4 0.2709603286346791 1105 5 0.27660428696466965 1105 6 0.1874397212380785 1105 7 0.2153878003296299 1105 8 0.20737209591118244 1105 9 0.26615634151467304 1105 10-11 0.3478748645078298 1105 12-13 0.24826704614376638 1105 14-15 0.3070347960226272 1105 16-17 0.46651450360509017 1105 18-19 0.3984116271125302 1105 20-21 0.4023427181901198 1105 22-23 0.4199517181265904 1105 24-25 0.4928661355372199 1105 26-27 0.3879286830889228 1105 28-29 0.3352459978312865 1105 30-31 0.273885222639322 1105 32-33 0.3285873388585543 1105 34-35 0.29763300033220474 1105 36-37 0.3553502055138722 1105 38-39 0.3340814786677413 1105 40-41 0.37942327523452235 1105 42-43 0.22855083353447014 1105 44-45 0.26229535622361766 1105 46-47 0.35475158233932547 1105 48-49 0.5122890471501407 1105 50-51 0.4325255455086179 1105 52-53 0.5215726858892111 1105 54-55 0.5691012452815372 1105 56-57 0.5577517246832144 1105 58-59 0.5723122458884191 1105 60-61 0.5838207066529861 1105 62-63 0.5941260144425655 1105 64-65 0.7326062939805524 1105 66-67 0.7672076428692733 1105 68-69 0.7330166614552169 1105 70-71 0.7528004794482115 1105 72-73 0.6559184320661089 1105 74-75 0.7077979583422191 1105 76-77 0.4518603094605389 1105 78-79 0.5687240436743899 1105 80-81 0.6046366947625081 1105 82-83 0.6284405582971537 1105 84-85 0.6248385115768684 1105 86-87 0.7597606868120756 1105 88-89 0.6844253820302946 1105 90-91 0.7368196267141478 1105 92-93 0.7707834759344117 1105 94-95 0.8349464145024044 1105 96-97 0.8957754872813375 1105 98-99 1.0642182605569737 1105 100-101 1.2356368436957048 1106 1 0.5566272946627251 1106 2 0.6010535393207768 1106 3 0.6422464664180296 1106 4 0.4680514161104483 1106 5 0.5551906153783648 1106 6 0.4974518713115401 1106 7 0.5587053959069195 1106 8 0.5239809471751116 1106 9 0.6338266074224634 1106 10-11 0.7176736029943243 1106 12-13 0.6936011493670406 1106 14-15 0.9123353760577544 1106 16-17 0.8787799415754591 1106 18-19 0.8909856851078857 1106 20-21 0.8557438378734403 1106 22-23 1.0272426630922666 1106 24-25 1.0659888234027193 1106 26-27 1.031685316505495 1106 28-29 0.9506948128421726 1106 30-31 0.9297694073503564 1106 32-33 1.0123916206885895 1106 34-35 1.0110745885310664 1106 36-37 1.0311124577444986 1106 38-39 1.0853972833500478 1106 40-41 0.9696154734867335 1106 42-43 1.1477459608586713 1106 44-45 1.1705046788043276 1106 46-47 0.8977699475119252 1106 48-49 1.1023396531919971 1106 50-51 1.014859546064784 1106 52-53 1.004835313772638 1106 54-55 1.018923464617906 1106 56-57 1.2130992018261821 1106 58-59 1.2596776652971187 1106 60-61 1.2955578340003058 1106 62-63 1.3385474003396425 1106 64-65 1.3055826302336655 1106 66-67 1.4179634869050304 1106 68-69 1.226882727122728 1106 70-71 1.234107333055121 1106 72-73 1.23898835516097 1106 74-75 1.2094642589881985 1106 76-77 1.242714081592613 1106 78-79 1.2165924604865062 1106 80-81 1.3052506746220764 1106 82-83 1.356791483286937 1106 84-85 1.5059673465500438 1106 86-87 1.427942911397377 1106 88-89 1.5592470744328608 1106 90-91 1.5972813533028152 1106 92-93 1.7329620378198918 1106 94-95 1.7332384180950733 1106 96-97 1.68015009845519 1106 98-99 1.6642539220764654 1106 100-101 1.7061341604128337 1107 1 0.3140314850490853 1107 2 0.36880608121543546 1107 3 0.41568440834036124 1107 4 0.4370159028484366 1107 5 0.2189778084137899 1107 6 0.2453516008167398 1107 7 0.4037745393945684 1107 8 0.36066638741238677 1107 9 0.450547466190649 1107 10-11 0.4135673160517399 1107 12-13 0.37560951364700657 1107 14-15 0.3438688069391347 1107 16-17 0.4571715538512464 1107 18-19 0.39547323069297846 1107 20-21 0.4289427881982135 1107 22-23 0.5482948633415674 1107 24-25 0.44010165633316234 1107 26-27 0.46107511525982403 1107 28-29 0.41828859493744375 1107 30-31 0.5576650954491953 1107 32-33 0.5436742696975543 1107 34-35 0.6400459688314939 1107 36-37 0.7109573406629792 1107 38-39 0.7713002718780331 1107 40-41 0.9240278305126068 1107 42-43 0.8909117757404559 1107 44-45 0.9177189707983189 1107 46-47 0.9684119245706384 1107 48-49 0.9781631834456377 1107 50-51 0.9708035313548891 1107 52-53 0.9188355666161101 1107 54-55 0.958788888512359 1107 56-57 1.0592312590383273 1107 58-59 1.0841054791688904 1107 60-61 1.0277170354238692 1107 62-63 0.9362501879558991 1107 64-65 1.1038149920165452 1107 66-67 1.013926186700779 1107 68-69 1.060288102670242 1107 70-71 1.1207752752977669 1107 72-73 0.9555177753043509 1107 74-75 1.0243949383056759 1107 76-77 0.9995736532853137 1107 78-79 1.010526994474187 1107 80-81 1.1090244655907249 1107 82-83 1.233235110438919 1107 84-85 1.2289790325525303 1107 86-87 1.151836056749655 1107 88-89 1.190115121493097 1107 90-91 1.1325519094425864 1107 92-93 1.2418410348423379 1107 94-95 1.3605492778923463 1107 96-97 1.3605392593079344 1107 98-99 1.3120775600100707 1107 100-101 1.5460503404182973 1108 1 -0.009441338280879563 1108 2 0.2603294324443901 1108 3 0.12418371869174649 1108 4 0.12647057552968022 1108 5 0.17821993150982962 1108 6 0.1030539541758202 1108 7 0.027278518684916264 1108 8 -0.1106610703444062 1108 9 0.09415505909718291 1108 10-11 0.017749328043670687 1108 12-13 -0.10142187576032313 1108 14-15 0.23507985604853587 1108 16-17 0.22804226047674803 1108 18-19 0.33564487657118747 1108 20-21 0.3132658158091104 1108 22-23 0.3216835150874289 1108 24-25 0.29289301059871065 1108 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-1.8495460848043521 2316 72-73 -1.541030022241344 2316 74-75 -1.768675695165495 2316 76-77 -1.941435966409351 2316 78-79 -2.2937175967098717 2316 80-81 -1.991877370243028 2316 82-83 -2.099394436672444 2316 84-85 -1.794425989325859 2316 86-87 -1.6281737073435707 2316 88-89 -2.041631012601922 2316 90-91 -2.0767373940490685 2316 92-93 -2.2684498601472143 2316 94-95 -2.5164280126119998 2316 96-97 -2.551823722609292 2316 98-99 -2.6631867587679814 2316 100-101 -2.483701861011081 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6079.0 3 1809.0 4 1864.0 5 1972.0 6 2255.0 7 2596.0 8 3316.0 9 4112.0 10 5119.0 11 6314.0 12 7750.0 13 9631.0 14 10478.0 15 12799.0 16 15716.0 17 20217.0 18 17803.0 19 12949.0 20 12782.0 21 13657.0 22 15106.0 23 17061.0 24 19944.0 25 23213.0 26 27093.0 27 32203.0 28 38928.0 29 47053.0 30 58200.0 31 72650.0 32 93138.0 33 121844.0 34 166506.0 35 235555.0 36 356946.0 37 525739.0 38 474516.0 39 81700.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.126496666480264 18.730078103155815 14.308465524633252 22.83495970573067 2 15.185310798993173 20.32123206166835 40.73438235107868 23.759074788259795 3 19.367875687914836 24.986154318182702 29.43642443520104 26.209545558701418 4 12.716098080401991 16.69034021906618 37.65287900839229 32.94068269213954 5 13.945438813993343 37.624932932019775 33.69168970904249 14.737938544944384 6 31.380043419680586 36.14731144529726 18.12565755019138 14.346987584830773 7 28.50066190067099 32.7705914831432 20.80396355524782 17.92478306093799 8 26.17046156475931 34.839883919240734 19.951943808831928 19.037710707168028 9 26.604720310765384 17.110359092246135 20.24801079612064 36.03690980086784 10-11 24.69747532498212 26.91067960184275 27.48588930501209 20.90595576816304 12-13 25.992179263160104 24.186965024229057 27.83828027699673 21.98257543561411 14-15 22.93187106899914 25.385145818522275 26.25850300494544 25.42448010753315 16-17 22.224240482709195 28.802025547810402 26.619293020404093 22.35444094907631 18-19 22.17287423780769 27.58855978794481 28.812340595877885 21.42622537836962 20-21 23.227352667467294 26.49410483436208 28.648104675894064 21.630437822276562 22-23 22.7674815681967 26.520939351968014 28.49716813238117 22.214410947454116 24-25 22.531926862643857 26.734600472210957 28.56307321211608 22.17039945302911 26-27 22.425707134340232 26.847077011078476 28.5417684819346 22.185447372646696 28-29 22.187877026073675 26.973594654577365 28.687274949477416 22.151253369871547 30-31 22.42379069272575 26.7846824961674 28.57161717811994 22.219909632986912 32-33 22.2386928112189 26.908444558347146 28.474982016640798 22.377880613793163 34-35 22.657155654042935 26.859958286099577 28.260245348247775 22.22264071160971 36-37 22.534411952464755 26.682543331412496 28.388676440665595 22.394368275457158 38-39 22.463432420845965 26.921438534469903 28.44281602589592 22.17231301878821 40-41 22.67634278244061 26.6998693293268 28.3784505437038 22.245337344528796 42-43 22.39715933882314 26.82489586462508 28.22072058514265 22.55722421140913 44-45 22.595578937190528 26.953113699477775 27.935021991149956 22.516285372181745 46-47 22.606617277354985 27.047520355522288 27.68510217654899 22.660760190573736 48-49 22.72241910664647 27.091842539227933 27.559983955871658 22.62575439825394 50-51 22.63298910518994 27.274604845489435 27.492687392305363 22.59971865701526 52-53 22.82742229949842 27.31484321672272 27.360543684133283 22.497190799645576 54-55 22.543336103877344 27.10984110046707 27.519869964992516 22.826952830663068 56-57 22.626561203365828 27.473296010332493 27.296065474334384 22.604077311967295 58-59 22.82650126241517 27.184653971253475 27.329429292808673 22.659415473522678 60-61 22.798081788446073 27.082233864456896 27.693936601469378 22.425747745627657 62-63 22.739841578805922 27.13434810098069 27.62271506399452 22.503095256218863 64-65 22.607514262561594 27.23664883288312 27.493652563106473 22.662184341448814 66-67 22.50065779700627 27.44622327426591 27.25943805202762 22.793680876700197 68-69 22.44075407923688 27.382535297466443 27.47138573282581 22.70532489047087 70-71 22.544351178880696 27.285165909784396 27.316513957354232 22.853968953980676 72-73 22.62797042309508 27.14476426051527 27.350562718938658 22.876702597450997 74-75 22.49657724839306 27.43248303377018 27.23592110113505 22.835018616701706 76-77 22.627775487972766 27.210940483426917 27.231597264219058 22.929686764381266 78-79 22.558615462796272 27.286087033012784 27.261911304007754 22.89338620018319 80-81 22.559687797211073 27.548786053844577 27.123355007395634 22.768171141548716 82-83 22.567542220076824 27.5027291060765 27.125605675142587 22.804122998704088 84-85 22.663223971380862 27.266822412575458 27.016193315482944 23.053760300560736 86-87 22.646687640191104 27.226983803030368 27.2311199928073 22.895208563971224 88-89 22.71210406965169 27.077921131985683 27.14578588805423 23.064188910308392 90-91 22.576784730901004 27.140779968045685 27.3628640201699 22.91957128088341 92-93 22.663265767852145 27.120459051426554 27.307555095162826 22.908720085558475 94-95 22.507640211352474 27.072111747620053 27.315141845245783 23.10510619578169 96-97 22.421353944204657 27.087935535086867 27.31216267111547 23.178547849593006 98-99 22.341222978557713 27.120650601370265 27.15620704810379 23.38191937196823 100-101 22.368425142938854 27.067610447806434 27.31406679987161 23.2498976093831 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 433.0 1 327.0 2 241.0 3 278.5 4 289.5 5 316.0 6 430.0 7 753.0 8 1272.0 9 1596.0 10 1589.5 11 1614.5 12 1727.0 13 1901.5 14 2163.5 15 2287.5 16 2416.0 17 2654.0 18 2775.0 19 2940.0 20 3263.0 21 3772.0 22 4466.0 23 5326.5 24 6640.5 25 8634.0 26 11259.5 27 14692.5 28 18146.0 29 21961.0 30 26727.5 31 31787.5 32 36864.5 33 42140.0 34 48404.5 35 53636.0 36 59310.0 37 66260.0 38 71460.0 39 75267.0 40 78859.5 41 82825.0 42 86863.0 43 91242.0 44 95234.5 45 97523.5 46 98932.5 47 102487.0 48 112036.0 49 112926.0 50 104818.0 51 100160.0 52 94946.0 53 90400.5 54 85930.0 55 79636.5 56 72934.5 57 64987.0 58 57964.5 59 51356.0 60 42840.5 61 34400.5 62 26594.0 63 19857.0 64 14622.0 65 11116.5 66 8172.0 67 5998.5 68 4304.0 69 2827.5 70 1958.5 71 1337.0 72 896.5 73 592.0 74 419.0 75 304.5 76 193.0 77 95.0 78 57.5 79 33.0 80 22.5 81 22.0 82 21.0 83 15.5 84 12.5 85 9.5 86 8.5 87 8.5 88 5.0 89 5.0 90 4.5 91 3.0 92 3.5 93 2.5 94 2.0 95 1.5 96 1.5 97 1.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06135905057639085 2 0.10129482732724865 3 0.07090637912907406 4 0.13358497918022602 5 0.10696112801298746 6 0.10436083934213472 7 0.11596511266429846 8 0.09962598534446257 9 0.12108806944866506 10-11 0.11177360256799854 12-13 0.09527923413348485 14-15 0.09884977977107369 16-17 0.1130737469034249 18-19 0.0932028842246696 20-21 0.0770384031588462 22-23 0.07804747040425174 24-25 0.06708356668013384 26-27 0.058506495094186727 28-29 0.06980028618699491 30-31 0.05282078926911319 32-33 0.05198636827772015 34-35 0.047988909574767424 36-37 0.05313127149846874 38-39 0.054528441530568725 40-41 0.07154674872711989 42-43 0.06766572086017549 44-45 0.054198554161878444 46-47 0.04059555148823835 48-49 0.048124745550110475 50-51 0.03017499166549266 52-53 0.04544683632191884 54-55 0.051637075769695145 56-57 0.05666300685738814 58-59 0.06258157435447834 60-61 0.059379726364249215 62-63 0.06710297181946856 64-65 0.04610661105929939 66-67 0.06809263392553937 68-69 0.05910805441356311 70-71 0.02790459036333019 72-73 0.022238289677591373 74-75 0.033318624237717615 76-77 0.04701865260803132 78-79 0.06754929002416717 80-81 0.04391383031447581 82-83 0.06279503088716028 84-85 0.0666178433361005 86-87 0.06999433758034214 88-89 0.0931252636673307 90-91 0.06640438680341856 92-93 0.07853259888761979 94-95 0.08788587604695579 96-97 0.09553150094483623 98-99 0.12667674957706498 100-101 0.12260167031677338 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2576637.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.246794873160745 #Duplication Level Percentage of deduplicated Percentage of total 1 74.27910955659965 41.036827290344945 2 14.19534471914109 15.684945957043869 3 4.933252734617531 8.17639205660622 4 2.2750318658990674 5.0275287530091965 5 1.2018196316639933 3.319834133253912 6 0.717521201410183 2.378444797887134 7 0.4942981374228704 1.9115871463070717 8 0.33874718338575477 1.4971756923499007 9 0.24131397935835416 1.1998641525873426 >10 1.2103588878821796 11.86548175681631 >50 0.07467179567176063 2.8150123806960647 >100 0.036488541775211276 3.6365186404598764 >500 0.0014785196074609402 0.5219962667978825 >1k 4.2243417355381874E-4 0.429693946292395 >5k 1.4081139118460626E-4 0.4986970295478226 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7401 0.2872348724325545 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5420 0.21035171038838613 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3408 0.1322654297054649 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 3.881027866944393E-5 0.0 8 0.0 0.0 0.0 3.881027866944393E-5 0.0 9 0.0 0.0 0.0 3.881027866944393E-5 0.0 10-11 0.0 0.0 0.0 1.3583597534305375E-4 0.0 12-13 3.881027866944393E-5 0.0 0.0 1.9405139334721964E-4 0.0 14-15 7.762055733888786E-5 0.0 0.0 1.9405139334721964E-4 0.0 16-17 7.762055733888786E-5 0.0 0.0 2.716719506861075E-4 0.0 18-19 9.702569667360982E-5 0.0 0.0 3.104822293555514E-4 0.0 20-21 1.164308360083318E-4 0.0 0.0 4.0750792602916127E-4 0.0 22-23 1.164308360083318E-4 0.0 0.0 6.209644587111028E-4 0.0 24-25 1.164308360083318E-4 0.0 0.0 7.179901553847126E-4 0.0 26-27 1.164308360083318E-4 0.0 0.0 0.001028472384740264 0.0 28-29 1.164308360083318E-4 0.0 0.0 0.001610626564781923 0.0 30-31 1.164308360083318E-4 0.0 0.0 0.0026779092281916314 0.0 32-33 1.164308360083318E-4 0.0 0.0 0.0043855614896471645 0.0 34-35 1.164308360083318E-4 0.0 0.0 0.005957377775759643 0.0 36-37 1.164308360083318E-4 0.0 0.0 0.008266589356591556 0.0 38-39 1.164308360083318E-4 0.0 0.0 0.010575800937423472 0.0 40-41 1.164308360083318E-4 0.0 0.0 0.014573259640376196 0.0 42-43 2.1345653268194163E-4 0.0 0.0 0.01913346738403586 0.0 44-45 2.328616720166636E-4 0.0 0.0 0.02776875438798713 0.0 46-47 2.716719506861075E-4 0.0 0.0 0.04723210914071326 0.0 48-49 2.716719506861075E-4 0.0 0.0 0.062426333239800555 0.0 50-51 2.716719506861075E-4 0.0 0.0 0.08018203573107116 0.0 52-53 2.716719506861075E-4 0.0 0.0 0.10263378194134448 0.0 54-55 2.716719506861075E-4 0.0 0.0 0.13094588023070383 1.9405139334721964E-5 56-57 3.104822293555514E-4 0.0 0.0 0.16020883034746453 3.881027866944393E-5 58-59 3.104822293555514E-4 0.0 0.0 0.1996594786149543 3.881027866944393E-5 60-61 3.104822293555514E-4 0.0 0.0 0.2492202044758342 3.881027866944393E-5 62-63 3.104822293555514E-4 0.0 0.0 0.3033411380803738 3.881027866944393E-5 64-65 3.104822293555514E-4 0.0 0.0 0.34387847415060796 3.881027866944393E-5 66-67 3.104822293555514E-4 0.0 0.0 0.41884052740063893 3.881027866944393E-5 68-69 3.104822293555514E-4 0.0 0.0 0.5149541825255168 3.881027866944393E-5 70-71 3.104822293555514E-4 0.0 0.0 0.5771088438146312 3.881027866944393E-5 72-73 3.104822293555514E-4 0.0 0.0 0.6911916579634616 3.881027866944393E-5 74-75 3.104822293555514E-4 0.0 0.0 0.7814061507305841 3.881027866944393E-5 76-77 3.104822293555514E-4 0.0 0.0 0.8531469508510512 3.881027866944393E-5 78-79 3.104822293555514E-4 0.0 0.0 0.9421195147007514 3.881027866944393E-5 80-81 3.104822293555514E-4 3.881027866944393E-5 0.0 1.0394750987430514 7.762055733888786E-5 82-83 3.104822293555514E-4 3.881027866944393E-5 0.0 1.165841366090761 7.762055733888786E-5 84-85 3.104822293555514E-4 3.881027866944393E-5 0.0 1.3646664237143222 7.762055733888786E-5 86-87 3.104822293555514E-4 3.881027866944393E-5 0.0 1.635135255761677 7.762055733888786E-5 88-89 3.104822293555514E-4 3.881027866944393E-5 0.0 1.9540393155884979 7.762055733888786E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13750 0.0 29.006283 1 GGTATCA 9570 0.0 28.214294 1 GTACATG 12505 0.0 24.785444 1 ATCAACG 16180 0.0 24.500202 3 TCAACGC 16295 0.0 24.327295 4 TATCAAC 16420 0.0 24.145386 2 CAACGCA 16535 0.0 23.913446 5 TACATGG 12815 0.0 23.741198 2 AACGCAG 16980 0.0 23.28538 6 ACATGGG 12935 0.0 22.52559 3 GAGTACT 7055 0.0 21.857613 12-13 ACGCAGA 18710 0.0 20.877924 7 CGCAGAG 18805 0.0 20.395374 8 GTACTTT 7760 0.0 19.872028 14-15 AGTACTT 7435 0.0 19.078684 12-13 GCAGAGT 19620 0.0 19.040218 9 CATGGGA 7715 0.0 19.037271 4 CATGGGG 5720 0.0 18.863052 4 AGAGTAC 15605 0.0 18.02816 10-11 GTGTAGC 1270 0.0 17.966784 1 >>END_MODULE