FastQCFastQC Report
Thu 26 May 2016
SRR936843_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936843_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2576637
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83750.32503608385659294No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63510.2464840798296384No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41370.16055812285548954No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA133800.030.8900531
GGTATCA90100.029.7905941
ATCAACG162850.025.1998083
TCAACGC163400.025.1144984
GTACATG122400.025.0342081
TATCAAC164350.025.0280972
CAACGCA166350.024.6976785
AACGCAG170600.024.165466
TACATGG127200.023.9732862
ACATGGG124650.023.4725173
ACGCAGA191300.021.4016237
AGTACTT89200.021.0056712-13
CGCAGAG192350.020.9637958
GAGTACT85700.020.64428712-13
CATGGGG54900.020.5040114
GCAGAGT201050.019.678659
AGAGTAC164700.019.04730810-11
CATGGGA75400.018.9608574
GTACTTT92050.018.91075314-15
TACTTTT105650.017.4205114-15
Produced by FastQC (version 0.11.5)