##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936843_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2576637 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18910385902244 33.0 31.0 34.0 31.0 34.0 2 32.18050738229716 33.0 31.0 34.0 30.0 34.0 3 32.36786439067669 34.0 31.0 34.0 31.0 34.0 4 36.08407548288719 37.0 35.0 37.0 35.0 37.0 5 36.01068679833442 37.0 35.0 37.0 35.0 37.0 6 36.05105414538408 37.0 35.0 37.0 35.0 37.0 7 35.994434994141585 37.0 35.0 37.0 35.0 37.0 8 35.96835060584785 37.0 35.0 37.0 35.0 37.0 9 37.67103476353091 39.0 37.0 39.0 35.0 39.0 10-11 37.598317108696335 39.0 37.0 39.0 35.0 39.0 12-13 37.50974487287111 39.0 37.0 39.0 35.0 39.0 14-15 38.74917246783307 40.0 38.0 41.0 35.0 41.0 16-17 38.662944372839476 40.0 38.0 41.0 34.0 41.0 18-19 38.70871546903968 40.0 38.0 41.0 34.5 41.0 20-21 38.78423231522329 40.0 38.0 41.0 35.0 41.0 22-23 38.719767666147774 40.0 38.0 41.0 34.0 41.0 24-25 38.62722863173974 40.0 38.0 41.0 34.0 41.0 26-27 38.479983792827625 40.0 38.0 41.0 34.0 41.0 28-29 38.31538066867782 40.0 38.0 41.0 34.0 41.0 30-31 38.2098582376951 40.0 38.0 41.0 34.0 41.0 32-33 38.224700064463875 40.0 38.0 41.0 34.0 41.0 34-35 38.16146822389029 40.0 38.0 41.0 34.0 41.0 36-37 38.02442253216111 40.0 37.5 41.0 33.0 41.0 38-39 37.88073407313486 40.0 37.0 41.0 33.0 41.0 40-41 37.71725722327204 40.0 37.0 41.0 33.0 41.0 42-43 37.51602185329172 40.0 37.0 41.0 33.0 41.0 44-45 37.218487509105856 40.0 37.0 41.0 32.0 41.0 46-47 36.9792991795119 40.0 36.5 41.0 31.0 41.0 48-49 36.69871929961418 39.5 36.0 41.0 31.0 41.0 50-51 36.46554869777931 39.0 36.0 41.0 30.0 41.0 52-53 36.214456673563255 39.0 35.5 40.5 30.0 41.0 54-55 35.98625902678569 39.0 35.0 40.0 29.0 41.0 56-57 35.724873158306735 38.5 35.0 40.0 28.5 41.0 58-59 35.41204581786259 38.0 34.5 40.0 28.0 41.0 60-61 35.09966673613707 38.0 34.0 40.0 28.0 41.0 62-63 34.73554385037551 37.5 34.0 40.0 27.0 41.0 64-65 34.34016743530424 37.0 34.0 39.5 26.0 41.0 66-67 33.96986420671597 36.0 33.0 39.0 26.0 40.5 68-69 33.54021850963096 36.0 33.0 39.0 26.0 40.0 70-71 33.07016413254952 35.0 32.0 38.0 25.0 40.0 72-73 32.73345197635523 35.0 32.0 37.5 25.0 39.5 74-75 32.29545877048261 35.0 32.0 37.0 24.0 39.0 76-77 30.86839007590126 33.5 30.0 35.5 21.5 38.0 78-79 31.59356052094261 34.5 31.0 36.0 23.0 38.5 80-81 31.435139680133446 34.5 31.0 36.0 22.5 37.5 82-83 31.11828344466062 34.0 31.0 35.0 21.5 37.0 84-85 30.749798283576617 34.0 31.0 35.0 20.0 36.5 86-87 30.407610191113456 34.0 31.0 35.0 18.5 36.0 88-89 30.0669095025803 34.0 30.0 35.0 17.5 36.0 90-91 29.671222993382457 34.0 30.0 35.0 12.0 35.0 92-93 29.250970353992432 34.0 29.0 35.0 4.5 35.0 94-95 28.807522169401434 34.0 29.0 35.0 2.0 35.0 96-97 28.228891574560173 34.0 29.0 35.0 2.0 35.0 98-99 27.54141658293349 33.0 28.0 35.0 2.0 35.0 100-101 24.55155440987613 31.0 21.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36376300830274744 1101 2 0.2093872335230813 1101 3 0.24004426153605607 1101 4 0.266958116566407 1101 5 0.30037411851304796 1101 6 0.2026895179366619 1101 7 0.31590638965784734 1101 8 0.31041851936105047 1101 9 0.28136897705515196 1101 10-11 0.38746820398534965 1101 12-13 0.4274233606614928 1101 14-15 0.5474897514532344 1101 16-17 0.509888064528397 1101 18-19 0.4907675333330914 1101 20-21 0.4977021173709488 1101 22-23 0.4952311893747563 1101 24-25 0.5124198156561164 1101 26-27 0.4748161625184224 1101 28-29 0.4197558165479762 1101 30-31 0.5397115209370824 1101 32-33 0.5581108977912734 1101 34-35 0.5213401605122314 1101 36-37 0.5314154705908436 1101 38-39 0.6312855892793223 1101 40-41 0.4642130842257828 1101 42-43 0.5364990316591829 1101 44-45 0.6606672096561468 1101 46-47 0.6320904471511142 1101 48-49 0.4249267381283204 1101 50-51 0.3735061711338048 1101 52-53 0.273460484534489 1101 54-55 0.29460803721718065 1101 56-57 0.3508607718338368 1101 58-59 0.33688185383078917 1101 60-61 0.28266586360986423 1101 62-63 0.3725953190185862 1101 64-65 0.3088265157017176 1101 66-67 0.4229111204306193 1101 68-69 0.3687337043080916 1101 70-71 0.5021098226141021 1101 72-73 0.4978683723779511 1101 74-75 0.3578792950677965 1101 76-77 0.2687744308843918 1101 78-79 0.49443613000479303 1101 80-81 0.6185221962871914 1101 82-83 0.7665750265083631 1101 84-85 0.725818165101284 1101 86-87 0.645004580942512 1101 88-89 0.6671344034800626 1101 90-91 0.8445669895828054 1101 92-93 0.8830732492789295 1101 94-95 0.8005886963762201 1101 96-97 0.8079526406750297 1101 98-99 0.9003866551492088 1101 100-101 1.0454795644081436 1102 1 0.19201320430703817 1102 2 0.03377294772936068 1102 3 0.150074120913402 1102 4 0.17219490643311985 1102 5 0.12267746087277231 1102 6 0.14323488250464322 1102 7 0.22405405178536597 1102 8 0.22223882929804972 1102 9 0.311350683321173 1102 10-11 0.26520044822059674 1102 12-13 0.30491789756312215 1102 14-15 0.39028040774788764 1102 16-17 0.4499788659496389 1102 18-19 0.32181322318479033 1102 20-21 0.38948689631703104 1102 22-23 0.29692766908205925 1102 24-25 0.26134943760415297 1102 26-27 0.26755482902198224 1102 28-29 0.25877373773478496 1102 30-31 0.25739305427449466 1102 32-33 0.2730636621468534 1102 34-35 0.3142623203961321 1102 36-37 0.37658737052578317 1102 38-39 0.3721303816815862 1102 40-41 0.43516552607784575 1102 42-43 0.44257961305518023 1102 44-45 0.532932185218165 1102 46-47 0.4890309452522388 1102 48-49 0.4200780645630502 1102 50-51 0.4638043756788832 1102 52-53 0.5416680322288698 1102 54-55 0.3383100439675104 1102 56-57 0.3894235738333549 1102 58-59 0.41239216004232304 1102 60-61 0.3497021118930874 1102 62-63 0.420114544120473 1102 64-65 0.3519488501823531 1102 66-67 0.3089589510384272 1102 68-69 0.2625716629720287 1102 70-71 0.14766733666802168 1102 72-73 0.36841101234725215 1102 74-75 0.20618865378623497 1102 76-77 0.20616565545248733 1102 78-79 0.32228875701835236 1102 80-81 0.29855561432403377 1102 82-83 0.43151750411383105 1102 84-85 0.42552568777383826 1102 86-87 0.3521074428832023 1102 88-89 0.39362500917502885 1102 90-91 0.5135268601365652 1102 92-93 0.31453291131748173 1102 94-95 0.469638923518783 1102 96-97 0.5690122871444494 1102 98-99 0.6301106366219393 1102 100-101 0.6165540571601547 1103 1 0.0868053047971955 1103 2 -0.1948293343365357 1103 3 0.027931408530733393 1103 4 0.14749922655473569 1103 5 0.1914478007012974 1103 6 0.14958839222515508 1103 7 0.21833608877506094 1103 8 0.29399733780949333 1103 9 0.2420452255092158 1103 10-11 0.3085273131609654 1103 12-13 0.2872448507362364 1103 14-15 0.28932213400177176 1103 16-17 0.37210594939401176 1103 18-19 0.39904223651697635 1103 20-21 0.33919137688369716 1103 22-23 0.39698690245703716 1103 24-25 0.37864085526930324 1103 26-27 0.35859038320034387 1103 28-29 0.37555111690939214 1103 30-31 0.3863904413207777 1103 32-33 0.4977678189927346 1103 34-35 0.4834389523930511 1103 36-37 0.4409596247336367 1103 38-39 0.5850364969328083 1103 40-41 0.6362332475623376 1103 42-43 0.6749229028352701 1103 44-45 0.8695750742168684 1103 46-47 0.8055775491502004 1103 48-49 0.6994458634694993 1103 50-51 0.5463147053832316 1103 52-53 0.6465139667324706 1103 54-55 0.7059994123446032 1103 56-57 0.7324728971364678 1103 58-59 0.7763954779545017 1103 60-61 0.7947276225965041 1103 62-63 0.7233448559494136 1103 64-65 0.5570865700377041 1103 66-67 0.7338762065005042 1103 68-69 0.7762039215824714 1103 70-71 0.6293445144732459 1103 72-73 0.7390790636354723 1103 74-75 0.6720507914937386 1103 76-77 0.5538643620449157 1103 78-79 0.7078460822475279 1103 80-81 0.6661224205068272 1103 82-83 0.6048656171830231 1103 84-85 0.5768851042693832 1103 86-87 0.4246760814762034 1103 88-89 0.6842640129220605 1103 90-91 1.0838268183088289 1103 92-93 0.9263465969072087 1103 94-95 0.8783966397424301 1103 96-97 1.1596308437833898 1103 98-99 1.0484784183636684 1103 100-101 1.015048921141716 1104 1 0.28216255016457836 1104 2 0.17680082175615297 1104 3 0.2635020733224991 1104 4 0.22522562590729223 1104 5 0.1915127552024245 1104 6 0.21678689581388966 1104 7 0.29669579451361017 1104 8 0.27331635130862253 1104 9 0.2738492314464125 1104 10-11 0.3247446526772677 1104 12-13 0.32816530868302607 1104 14-15 0.37845945922807545 1104 16-17 0.4054012755665539 1104 18-19 0.3941835959444546 1104 20-21 0.3312028510085412 1104 22-23 0.36976438325815764 1104 24-25 0.48194747825846207 1104 26-27 0.32344560927273136 1104 28-29 0.41665283302486245 1104 30-31 0.412496664751977 1104 32-33 0.4642080665571555 1104 34-35 0.3828525185794902 1104 36-37 0.41563746357449105 1104 38-39 0.3744932841954096 1104 40-41 0.45285242722209773 1104 42-43 0.4801388370521593 1104 44-45 0.4911955749107335 1104 46-47 0.518624582353354 1104 48-49 0.6047178428877018 1104 50-51 0.5045689302785306 1104 52-53 0.4562758382647587 1104 54-55 0.4191017446500709 1104 56-57 0.3803778072578652 1104 58-59 0.4065364292219442 1104 60-61 0.39462830365322077 1104 62-63 0.5469841688712407 1104 64-65 0.41840761292610296 1104 66-67 0.5806274720160687 1104 68-69 0.61038649112988 1104 70-71 0.6390361210350548 1104 72-73 0.6238166430620993 1104 74-75 0.623555892616487 1104 76-77 0.5956239619251882 1104 78-79 0.6292234863321085 1104 80-81 0.7020231682735805 1104 82-83 0.6407398126030301 1104 84-85 0.7155140288598396 1104 86-87 0.5953714632989495 1104 88-89 0.7295318141858651 1104 90-91 0.7656577276981764 1104 92-93 0.9511809409852461 1104 94-95 0.914441560168779 1104 96-97 0.912051715595684 1104 98-99 0.9362723703974005 1104 100-101 0.9298426598789113 1105 1 0.17033172622029014 1105 2 0.03201273800962667 1105 3 0.06082506060828763 1105 4 -0.013081686556674299 1105 5 0.048420771959676756 1105 6 0.050532930383276664 1105 7 0.12535096935071266 1105 8 0.0868097028685284 1105 9 0.1764156236761636 1105 10-11 0.1535865024034564 1105 12-13 0.21712881235023218 1105 14-15 0.1954765344501581 1105 16-17 0.20258762375577533 1105 18-19 0.1516601848366932 1105 20-21 0.2048562298240455 1105 22-23 0.20647441778962872 1105 24-25 0.24841689103990205 1105 26-27 0.21938711208910888 1105 28-29 0.21679889239395322 1105 30-31 0.29991996891105543 1105 32-33 0.2247512099756932 1105 34-35 0.3190815624233423 1105 36-37 0.11965490883571306 1105 38-39 0.23082728131183927 1105 40-41 0.039688529378182125 1105 42-43 0.09373942321582973 1105 44-45 0.2872347867198499 1105 46-47 0.13354129796481118 1105 48-49 0.16881203778772402 1105 50-51 0.1539338970675601 1105 52-53 0.2141899581848321 1105 54-55 0.1678986659966668 1105 56-57 0.2746445029979654 1105 58-59 0.11908554142145533 1105 60-61 0.3458901310988054 1105 62-63 0.1795007675870366 1105 64-65 0.17635867656666449 1105 66-67 0.2450591564555964 1105 68-69 0.17182623080530846 1105 70-71 -0.023671849162575143 1105 72-73 0.10927112531354055 1105 74-75 0.13632141526178287 1105 76-77 0.101525175770945 1105 78-79 0.06231855900288963 1105 80-81 0.17857978177891098 1105 82-83 0.41896929194637167 1105 84-85 0.4832695496135635 1105 86-87 0.4356008574685859 1105 88-89 0.41730861933806196 1105 90-91 0.5254227957325597 1105 92-93 0.6116545308900854 1105 94-95 0.6027786157136283 1105 96-97 0.9744593656996727 1105 98-99 0.9575396137472687 1105 100-101 1.0206017186522338 1106 1 0.38703783926425217 1106 2 0.28335386950986674 1106 3 0.29499307973836864 1106 4 0.24457245177738685 1106 5 0.2071951035526567 1106 6 0.2439142339562963 1106 7 0.2706497340139151 1106 8 0.27818525844372743 1106 9 0.45748992950037604 1106 10-11 0.41335058564403937 1106 12-13 0.38969733584094257 1106 14-15 0.5603110466420489 1106 16-17 0.6081396701966639 1106 18-19 0.49062879182091024 1106 20-21 0.48109226352876533 1106 22-23 0.5282751817416411 1106 24-25 0.5583229744395837 1106 26-27 0.5960559624147521 1106 28-29 0.5356401581773156 1106 30-31 0.5952870989848336 1106 32-33 0.6730148951262933 1106 34-35 0.5633969666359562 1106 36-37 0.5092966992411618 1106 38-39 0.7301476217042762 1106 40-41 0.7749197649257411 1106 42-43 0.6107736630339318 1106 44-45 0.6575964094855635 1106 46-47 0.49357221368055804 1106 48-49 0.5462925361379121 1106 50-51 0.4928193844949149 1106 52-53 0.4078498567551705 1106 54-55 0.5255442714028007 1106 56-57 0.5279137872450903 1106 58-59 0.49894378603160305 1106 60-61 0.5626507222981303 1106 62-63 0.4819379893744937 1106 64-65 0.5468376695859831 1106 66-67 0.6504242263882105 1106 68-69 0.6207354948453769 1106 70-71 0.612187661448516 1106 72-73 0.7257605495822688 1106 74-75 0.6387224485959564 1106 76-77 0.6332787162561146 1106 78-79 0.7695214210353072 1106 80-81 0.8274534522909498 1106 82-83 0.8816288933940477 1106 84-85 0.7806072421058765 1106 86-87 0.5883357628550705 1106 88-89 0.9442331515398372 1106 90-91 1.0697141309692029 1106 92-93 1.0742768220254248 1106 94-95 1.127163242029173 1106 96-97 1.4847527624373065 1106 98-99 1.4546555860965036 1106 100-101 1.401583437109128 1107 1 0.16373310018712317 1107 2 0.07735169955407883 1107 3 0.050463460591508635 1107 4 0.09289141755900943 1107 5 0.191719856655439 1107 6 0.16680515483128744 1107 7 0.18895914490227028 1107 8 0.15774589691777408 1107 9 0.16075358662555317 1107 10-11 0.13611965096148282 1107 12-13 0.21022796976927083 1107 14-15 0.26833903116611424 1107 16-17 0.2791913422689092 1107 18-19 0.20936726989407362 1107 20-21 0.21903772975916525 1107 22-23 0.2790930894653627 1107 24-25 0.2617266836736931 1107 26-27 0.3630425623925291 1107 28-29 0.3022524339661601 1107 30-31 0.29838821388140246 1107 32-33 0.2541518354204797 1107 34-35 0.2685201477475303 1107 36-37 0.28967701738775986 1107 38-39 0.32470077028695243 1107 40-41 0.3263936855874192 1107 42-43 0.3335318240509224 1107 44-45 0.49943762446998363 1107 46-47 0.4492051708382405 1107 48-49 0.4190022922836718 1107 50-51 0.43183921396529 1107 52-53 0.44919811216932715 1107 54-55 0.38850209016106163 1107 56-57 0.38970084193220345 1107 58-59 0.36708677003431234 1107 60-61 0.4238230470472857 1107 62-63 0.3515133554693435 1107 64-65 0.5354232523288331 1107 66-67 0.3892822462626597 1107 68-69 0.4692148731094292 1107 70-71 0.4874128182148638 1107 72-73 0.42151080293687926 1107 74-75 0.45783739764220144 1107 76-77 0.6462883985933985 1107 78-79 0.6071235892191424 1107 80-81 0.6641134207638935 1107 82-83 0.6752784673425829 1107 84-85 0.6908592268589366 1107 86-87 0.6387336505557712 1107 88-89 0.6703575893720313 1107 90-91 0.8308741713423728 1107 92-93 0.9280953887778054 1107 94-95 1.016279966431842 1107 96-97 1.2484717354430295 1107 98-99 1.2074593543501315 1107 100-101 1.1378176948772492 1108 1 0.03150055138601715 1108 2 -0.10409004028503688 1108 3 -0.021400182630273434 1108 4 0.023142459160091278 1108 5 0.02132185039929624 1108 6 0.06555392671025118 1108 7 0.043603521444474325 1108 8 0.15423899508548544 1108 9 0.13701654649525352 1108 10-11 0.10879811610422507 1108 12-13 0.13860007529441276 1108 14-15 0.15468199859165566 1108 16-17 0.1721859141818527 1108 18-19 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-0.9548798404463916 2316 84-85 -0.9519039207079594 2316 86-87 -0.9700488697712828 2316 88-89 -1.160317128152336 2316 90-91 -1.1360581687848708 2316 92-93 -1.3954271239699416 2316 94-95 -1.185264747886695 2316 96-97 -1.0460006538783269 2316 98-99 -1.095145087131673 2316 100-101 -1.087980139611382 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 14.0 4 15.0 5 48.0 6 101.0 7 246.0 8 608.0 9 1130.0 10 1845.0 11 3051.0 12 4789.0 13 6682.0 14 8362.0 15 11351.0 16 15205.0 17 21003.0 18 18556.0 19 11544.0 20 10566.0 21 10720.0 22 11453.0 23 12822.0 24 15088.0 25 18484.0 26 22778.0 27 29035.0 28 35938.0 29 45837.0 30 57994.0 31 73731.0 32 94299.0 33 125081.0 34 171480.0 35 246255.0 36 383445.0 37 595708.0 38 471677.0 39 39694.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.19488269742928 19.190660977152017 14.865299718461408 22.7491566069573 2 15.480471313694357 20.723310264955796 40.004843699484155 23.791374721865694 3 19.507445945578812 24.83697318528121 29.784248050703066 25.871332818436915 4 13.098762916606024 17.121622277203706 37.19842817639354 32.58118662979673 5 13.941948877412052 37.57332847937449 33.801678264031814 14.68304437918164 6 31.63537771071338 36.22358369155346 17.73845506607861 14.40258353165455 7 28.562097464328474 32.80491804233041 20.64091343942778 17.992071053913342 8 26.504354319215317 34.63305075569434 19.781948330323594 19.080646594766744 9 26.558999191581893 16.76018003312069 20.239211033606985 36.44160974169043 10-11 24.794688415984815 26.856410450461986 27.382174213823664 20.96672691972953 12-13 26.025743027687437 24.060067064604016 27.933125567604073 21.981064340104478 14-15 22.882423872668134 25.271642842977105 26.350471564290974 25.49546172006379 16-17 22.194705734645588 28.702781959585305 26.846796813055157 22.25571549271395 18-19 22.159757078703752 27.242137716721448 29.195963575777263 21.402141628797537 20-21 23.086918427801166 26.22037136400639 29.15835219714486 21.53435801104758 22-23 22.68288082488919 26.261867698088633 28.99950594515254 22.05574553186964 24-25 22.486971884420328 26.472156684363508 29.112884978050047 21.927986453166124 26-27 22.451291622688874 26.542444076644877 29.029690274081833 21.97657402658442 28-29 22.14704461172142 26.650676385032007 29.191539899051644 22.010739104194926 30-31 22.420946069739493 26.489393746487096 29.05311511146581 22.0365450723076 32-33 22.272794573814146 26.526396147816335 29.011593304630317 22.189215973739206 34-35 22.572294503681043 26.53667906165501 28.837093681195103 22.053932753468843 36-37 22.555030670001113 26.37173760707429 28.8441807920349 22.229050930889695 38-39 22.461232032623098 26.55221777554312 29.01785099198375 21.968699199850032 40-41 22.66277330221126 26.406834333357722 28.889562493292797 22.040829871138225 42-43 22.408015592774603 26.534748802481428 28.672972446399836 22.38426315834413 44-45 22.63944625129045 26.636737275966187 28.36984110967081 22.353975363072554 46-47 22.60141432637089 26.885881691339048 28.00358064186867 22.509123340421393 48-49 22.765526527765623 26.94713114666616 27.85999001409011 22.42735231147811 50-51 22.759465015105953 27.023847202172913 27.805118578175748 22.41156920454539 52-53 22.95551448528961 27.102080482369495 27.57314444460831 22.36926058773258 54-55 22.671036826161963 26.92780892389674 27.752104061026987 22.64905018891431 56-57 22.703956488868396 27.276696519637294 27.536997967886713 22.482349023607597 58-59 22.92142218568845 26.985131441041847 27.590053836210853 22.503392537058847 60-61 22.844050369981687 26.866131287030186 27.982965240427944 22.306853102560183 62-63 22.7412902942867 26.930665825259826 27.904667983887528 22.42337589656595 64-65 22.660595695895438 26.994506591643706 27.76838459951716 22.57651311294369 66-67 22.551365918850376 27.225620387516976 27.558903849613188 22.66410984401946 68-69 22.52870699287482 27.083869400307453 27.729226119162302 22.65819748765542 70-71 22.608443384921987 27.065268329884063 27.586821170655885 22.739467114538066 72-73 22.71743078960319 26.930850923452127 27.58558057870805 22.76613770823663 74-75 22.592931177981114 27.166492440012117 27.451225430791332 22.789350951215436 76-77 22.66778570700872 27.048731619519817 27.474154534296485 22.809328139174983 78-79 22.667718169748145 27.0615987012488 27.47510409919317 22.79557902980988 80-81 22.559361711087483 27.3140816067915 27.39824573013105 22.728310951989965 82-83 22.6080287790837 27.239318115178047 27.381055645758636 22.77159745997962 84-85 22.647107452129823 27.054004731905273 27.306338082833182 22.99254973313172 86-87 22.63784252872158 26.960764622861106 27.446969163318734 22.954423685098583 88-89 22.682894455464528 26.82226911417293 27.426008369915657 23.06882806044689 90-91 22.519673457380303 26.85011418978616 27.612272069172484 23.01794028366105 92-93 22.569626165594627 26.778079398502424 27.580171894705323 23.072122541197622 94-95 22.408626304780604 26.78428280655686 27.56978542324947 23.237305465413066 96-97 22.33874324569515 26.68860774723254 27.57988005499501 23.392768952077304 98-99 22.22422100393566 26.759185032656795 27.434326921901388 23.582267041506157 100-101 22.121206593627516 26.841693869733437 27.443764265358073 23.593335271280974 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 415.0 1 303.0 2 222.5 3 263.5 4 236.0 5 234.0 6 383.5 7 840.0 8 1451.5 9 1764.0 10 1663.5 11 1551.5 12 1670.5 13 1861.5 14 2120.5 15 2437.5 16 2615.5 17 2798.5 18 3117.0 19 3401.5 20 3763.5 21 4220.0 22 4882.5 23 5868.5 24 7164.5 25 8957.0 26 11627.0 27 14996.0 28 18178.5 29 21994.5 30 26501.5 31 31478.0 32 36382.0 33 41341.0 34 48055.0 35 53439.0 36 58775.0 37 65664.5 38 71185.0 39 74923.5 40 77959.0 41 82058.0 42 86483.5 43 90481.5 44 94605.0 45 97573.5 46 99032.5 47 102816.0 48 113168.0 49 113792.0 50 104794.0 51 99921.5 52 95197.5 53 90855.5 54 86218.5 55 79825.5 56 72394.5 57 64610.5 58 58283.5 59 51777.0 60 43319.5 61 34667.0 62 26662.0 63 19911.5 64 14533.0 65 10827.0 66 7989.0 67 5997.0 68 4244.0 69 2692.5 70 1759.5 71 1198.5 72 823.5 73 558.0 74 392.5 75 291.5 76 188.0 77 91.5 78 44.0 79 20.0 80 12.5 81 8.5 82 8.5 83 5.0 84 2.0 85 0.5 86 0.5 87 1.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.016921281499877556 2 0.0036481661949277293 3 0.0026002886708527432 4 4.6572334403332714E-4 5 0.0017852728187944206 6 3.492925080249954E-4 7 7.762055733888786E-5 8 0.0 9 0.0 10-11 1.746462540124977E-4 12-13 6.597747373805468E-4 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0021151601874846945 22-23 0.0 24-25 0.0036481661949277293 26-27 0.0020375396301458063 28-29 0.0027943400641999632 30-31 0.016339127319835892 32-33 7.956107127236006E-4 34-35 0.0022509961628277477 36-37 0.0037451918916013393 38-39 0.0022315910234930263 40-41 0.004521397464990217 42-43 0.002289806441497192 44-45 6.597747373805468E-4 46-47 1.552411146777757E-4 48-49 2.5226681135138556E-4 50-51 1.3583597534305375E-4 52-53 8.926364093972103E-4 54-55 0.0027943400641999632 56-57 3.492925080249954E-4 58-59 9.896621060708202E-4 60-61 8.926364093972103E-4 62-63 0.0 64-65 1.3583597534305375E-4 66-67 5.821541800416589E-5 68-69 0.0 70-71 1.746462540124977E-4 72-73 3.881027866944393E-5 74-75 4.8512848336804907E-4 76-77 9.508518274013762E-4 78-79 3.104822293555514E-4 80-81 0.0047930694156763255 82-83 0.001688247122120811 84-85 0.003298873686902734 86-87 0.0018046779581291428 88-89 0.00108668780274443 90-91 2.1345653268194163E-4 92-93 5.821541800416589E-5 94-95 2.5226681135138556E-4 96-97 0.0015330060074430353 98-99 0.002619693810187465 100-101 0.00108668780274443 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2576637.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.48716716578985 #Duplication Level Percentage of deduplicated Percentage of total 1 73.05413577972263 39.07458772602334 2 14.744688943466489 15.77303284653531 3 5.220127789604277 8.376295431280472 4 2.3154713168285697 4.953920055632052 5 1.2925037078449144 3.4566180941952074 6 0.7821399780666353 2.5100671052338397 7 0.5180925902124023 1.939791348603348 8 0.35176981139819746 1.5052136564907006 9 0.2783819100877013 1.3400873784713483 >10 1.3218006073037687 12.602983452978195 >50 0.07868449018959685 2.877891846378269 >100 0.03965735630404647 3.8725307975974657 >500 0.0018911055211303762 0.6381779171338692 >1k 5.091437941425595E-4 0.505905000871962 >5k 1.454696554693027E-4 0.572897342574632 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8375 0.32503608385659294 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6351 0.2464840798296384 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4137 0.16055812285548954 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 3.881027866944393E-5 0.0 7 0.0 0.0 0.0 3.881027866944393E-5 0.0 8 0.0 0.0 0.0 3.881027866944393E-5 0.0 9 0.0 0.0 0.0 1.164308360083318E-4 0.0 10-11 0.0 0.0 0.0 1.9405139334721964E-4 0.0 12-13 3.881027866944393E-5 0.0 0.0 1.9405139334721964E-4 0.0 14-15 3.881027866944393E-5 0.0 0.0 1.9405139334721964E-4 0.0 16-17 3.881027866944393E-5 0.0 0.0 2.1345653268194163E-4 0.0 18-19 7.762055733888786E-5 0.0 0.0 2.328616720166636E-4 0.0 20-21 7.762055733888786E-5 0.0 0.0 2.716719506861075E-4 0.0 22-23 7.762055733888786E-5 0.0 0.0 4.851284833680491E-4 0.0 24-25 1.3583597534305375E-4 0.0 0.0 6.015593193763809E-4 0.0 26-27 1.552411146777757E-4 0.0 0.0 8.344209913930445E-4 0.0 28-29 1.9405139334721964E-4 0.0 0.0 0.0014165751714347035 0.0 30-31 1.9405139334721964E-4 0.0 0.0 0.0024644526955096896 0.0 32-33 1.9405139334721964E-4 0.0 0.0 0.004133294678295779 0.0 34-35 1.9405139334721964E-4 0.0 0.0 0.005763326382412424 0.0 36-37 1.9405139334721964E-4 0.0 0.0 0.008033727684574894 0.0 38-39 1.9405139334721964E-4 0.0 0.0 0.01047877524074986 0.0 40-41 1.9405139334721964E-4 0.0 0.0 0.014495639083037307 0.0 42-43 2.1345653268194163E-4 0.0 0.0 0.018997631408692803 0.0 44-45 2.716719506861075E-4 0.0 0.0 0.027594108133974636 0.0 46-47 3.881027866944393E-4 0.0 0.0 0.0469992474686966 0.0 48-49 3.881027866944393E-4 0.0 0.0 0.062368117821796396 0.0 50-51 3.881027866944393E-4 0.0 0.0 0.08031787170641422 0.0 52-53 3.881027866944393E-4 0.0 0.0 0.10271140249868335 0.0 54-55 4.269130653638832E-4 0.0 0.0 0.13090706995203438 0.0 56-57 4.269130653638832E-4 0.0 0.0 0.16003418409345205 0.0 58-59 4.269130653638832E-4 0.0 0.0 0.19925197068892514 0.0 60-61 4.657233440333272E-4 0.0 0.0 0.2488709119678092 0.0 62-63 4.657233440333272E-4 0.0 0.0 0.30299184557234876 0.0 64-65 4.657233440333272E-4 0.0 0.0 0.3435873970605871 0.0 66-67 4.657233440333272E-4 0.0 0.0 0.4185106400319486 0.0 68-69 4.657233440333272E-4 0.0 0.0 0.5145466745994876 0.0 70-71 5.045336227027711E-4 0.0 0.0 0.5766043101919285 0.0 72-73 5.045336227027711E-4 0.0 0.0 0.6902019958573908 0.0 74-75 5.045336227027711E-4 0.0 0.0 0.7803582732065091 0.0 76-77 5.045336227027711E-4 0.0 0.0 0.8524483658350012 0.0 78-79 5.045336227027711E-4 0.0 0.0 0.941731411914057 0.0 80-81 5.045336227027711E-4 0.0 0.0 1.0387765137270013 0.0 82-83 5.045336227027711E-4 0.0 0.0 1.1656473146974138 0.0 84-85 5.045336227027711E-4 0.0 0.0 1.364666423714322 0.0 86-87 5.045336227027711E-4 0.0 0.0 1.6349994197863338 0.0 88-89 5.045336227027711E-4 0.0 0.0 1.9548931417192255 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13380 0.0 30.890053 1 GGTATCA 9010 0.0 29.790594 1 ATCAACG 16285 0.0 25.199808 3 TCAACGC 16340 0.0 25.114498 4 GTACATG 12240 0.0 25.034208 1 TATCAAC 16435 0.0 25.028097 2 CAACGCA 16635 0.0 24.697678 5 AACGCAG 17060 0.0 24.16546 6 TACATGG 12720 0.0 23.973286 2 ACATGGG 12465 0.0 23.472517 3 ACGCAGA 19130 0.0 21.401623 7 AGTACTT 8920 0.0 21.00567 12-13 CGCAGAG 19235 0.0 20.963795 8 GAGTACT 8570 0.0 20.644287 12-13 CATGGGG 5490 0.0 20.504011 4 GCAGAGT 20105 0.0 19.67865 9 AGAGTAC 16470 0.0 19.047308 10-11 CATGGGA 7540 0.0 18.960857 4 GTACTTT 9205 0.0 18.910753 14-15 TACTTTT 10565 0.0 17.42051 14-15 >>END_MODULE