FastQCFastQC Report
Thu 26 May 2016
SRR936842_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936842_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357284
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69220.29364302307231543No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49270.2090117270553739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31120.13201633744597596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA125350.028.851321
GGTATCA87550.028.0090831
GTACATG110900.025.9256211
TACATGG113250.024.7582042
TATCAAC148650.024.1052572
ATCAACG148100.023.9696463
TCAACGC148900.023.9690384
ACATGGG114150.023.6465913
CAACGCA151050.023.5659455
AACGCAG155700.022.8301666
GAGTACT65750.022.69402712-13
ACGCAGA170400.020.6101067
GTACTTT71900.020.58612414-15
CGCAGAG170350.020.3668068
CATGGGG48000.020.1973444
AGTACTT69650.019.9564112-13
CATGGGA69400.019.8583834
GCAGAGT177150.018.9142959
AGAGTAC142250.018.17425310-11
ATGGGAG30300.017.0965185
Produced by FastQC (version 0.11.5)