##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936842_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2357284 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.860521685125764 33.0 31.0 34.0 30.0 34.0 2 31.978074343184783 33.0 31.0 34.0 30.0 34.0 3 32.00224962287107 33.0 31.0 34.0 30.0 34.0 4 35.57905369060325 37.0 35.0 37.0 33.0 37.0 5 35.58381255716324 37.0 35.0 37.0 33.0 37.0 6 35.653226340143995 37.0 35.0 37.0 33.0 37.0 7 35.59267360233218 37.0 35.0 37.0 33.0 37.0 8 35.54039352067888 37.0 35.0 37.0 33.0 37.0 9 37.195343030368846 39.0 37.0 39.0 34.0 39.0 10-11 37.00767514648214 39.0 37.0 39.0 33.0 39.0 12-13 36.94779521686823 39.0 37.0 39.0 33.0 39.0 14-15 38.14642189910083 40.0 38.0 41.0 33.0 41.0 16-17 38.08302414982667 40.0 38.0 41.0 33.0 41.0 18-19 38.04022871236559 40.0 38.0 41.0 33.0 41.0 20-21 37.95950275825908 40.0 38.0 41.0 33.0 41.0 22-23 37.85468933738998 40.0 38.0 41.0 33.0 41.0 24-25 37.705025996019145 40.0 37.0 41.0 32.0 41.0 26-27 37.81054722299052 40.0 38.0 41.0 33.0 41.0 28-29 37.77227245423123 40.0 38.0 41.0 33.0 41.0 30-31 37.650980111009105 40.0 37.0 41.0 32.5 41.0 32-33 37.4784400182583 40.0 37.0 41.0 32.0 41.0 34-35 37.33030131286684 40.0 37.0 41.0 31.5 41.0 36-37 37.17798300925981 40.0 36.5 41.0 31.0 41.0 38-39 37.032361819789216 39.5 36.0 41.0 31.0 41.0 40-41 36.873352765301085 39.0 36.0 41.0 30.5 41.0 42-43 36.671501821587896 39.0 36.0 41.0 30.0 41.0 44-45 36.44918092177268 39.0 36.0 41.0 30.0 41.0 46-47 36.13953834158294 39.0 35.0 40.5 29.5 41.0 48-49 35.861951508600576 39.0 35.0 40.0 28.5 41.0 50-51 35.23580081992666 38.5 34.0 39.5 27.5 40.5 52-53 35.476451288856154 38.5 35.0 40.0 28.0 40.5 54-55 35.86457465456008 39.0 35.0 40.5 28.0 41.0 56-57 35.763621184379986 39.0 35.0 41.0 28.0 41.0 58-59 35.52869891790722 39.0 35.0 41.0 28.0 41.0 60-61 35.248038632595815 38.0 35.0 40.0 27.5 41.0 62-63 34.93060042828951 38.0 34.0 40.0 26.5 41.0 64-65 34.61634300321896 37.5 34.0 40.0 26.0 41.0 66-67 34.24483939991957 37.0 34.0 40.0 26.0 41.0 68-69 33.87051793504728 36.0 34.0 39.0 26.0 41.0 70-71 33.46579707833252 36.0 33.0 39.0 25.5 40.5 72-73 33.06885105910022 35.0 33.0 38.5 24.5 40.0 74-75 32.63465539154383 35.0 32.5 37.5 23.5 39.5 76-77 32.2054222147183 35.0 32.0 37.0 22.5 39.0 78-79 31.763772629857073 35.0 32.0 36.5 21.0 39.0 80-81 31.347636093063034 35.0 31.0 36.0 20.0 37.5 82-83 30.950722950649986 35.0 31.0 35.5 18.5 37.0 84-85 30.60067115375152 34.0 31.0 35.0 18.0 37.0 86-87 30.28295063301664 34.0 31.0 35.0 15.5 36.0 88-89 29.95248387551097 34.0 30.5 35.0 9.0 36.0 90-91 29.62107344723843 34.0 30.0 35.0 6.0 36.0 92-93 29.27182320840425 34.0 30.0 35.0 2.0 35.0 94-95 28.876750531543927 34.0 29.0 35.0 2.0 35.0 96-97 28.41965562910536 34.0 29.0 35.0 2.0 35.0 98-99 27.840767171032425 34.0 29.0 35.0 2.0 35.0 100-101 25.617819490566262 32.0 23.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26970443566059643 1101 2 0.3097389849901653 1101 3 0.2593771581132742 1101 4 0.15352177217249618 1101 5 0.052660728525729894 1101 6 0.023175446603687533 1101 7 0.035625497448357635 1101 8 0.042859152928471644 1101 9 0.11636344787858377 1101 10-11 0.2591368787712014 1101 12-13 0.21132017101611922 1101 14-15 0.44670575621091047 1101 16-17 0.5466583588540388 1101 18-19 0.4381091818363174 1101 20-21 0.5870957060367274 1101 22-23 0.47582871860784337 1101 24-25 0.5190848316267491 1101 26-27 0.517674758426665 1101 28-29 0.583029759117359 1101 30-31 0.4614165093497533 1101 32-33 0.5410377078039303 1101 34-35 0.4557842270446031 1101 36-37 0.44172762751575334 1101 38-39 0.4763897138306348 1101 40-41 0.5424634962741379 1101 42-43 0.5007363757975227 1101 44-45 0.517409865494237 1101 46-47 0.4570501581068953 1101 48-49 0.4985818352276894 1101 50-51 0.48525112817906546 1101 52-53 0.5741197068593706 1101 54-55 0.6555845790354553 1101 56-57 0.5040132323399504 1101 58-59 0.4750405808245475 1101 60-61 0.5281553729982917 1101 62-63 0.592422343726625 1101 64-65 0.6398354693474602 1101 66-67 0.5274592405361531 1101 68-69 0.6161548735649163 1101 70-71 0.6264832233802125 1101 72-73 0.6021937873853744 1101 74-75 0.46255895522764234 1101 76-77 0.5817073543467615 1101 78-79 0.5622775405530547 1101 80-81 0.3606328534926462 1101 82-83 0.4302285045021179 1101 84-85 0.4152748863600344 1101 86-87 0.3533871837826794 1101 88-89 0.34785944555166637 1101 90-91 0.5375236284738598 1101 92-93 0.6297588369564338 1101 94-95 0.559657827629163 1101 96-97 0.750320038843995 1101 98-99 0.5417132668748614 1101 100-101 0.684880301804224 1102 1 0.37647251598172815 1102 2 0.23288476949089798 1102 3 0.30052434076856827 1102 4 0.2114487075252427 1102 5 0.22043026077659533 1102 6 0.20757068149048763 1102 7 0.23170528715578342 1102 8 0.26284849279074507 1102 9 0.3527357112757201 1102 10-11 0.5040734715128181 1102 12-13 0.4804120190158372 1102 14-15 0.4993111634629699 1102 16-17 0.5007648843134191 1102 18-19 0.40103277996494313 1102 20-21 0.47104547685957954 1102 22-23 0.5034410850002615 1102 24-25 0.5812362024192268 1102 26-27 0.5016196262188117 1102 28-29 0.448811191498244 1102 30-31 0.48393952986972266 1102 32-33 0.5279349118111369 1102 34-35 0.4043634552759343 1102 36-37 0.2999596579082109 1102 38-39 0.5014855601728172 1102 40-41 0.3827686229463012 1102 42-43 0.4292463922147718 1102 44-45 0.40616286662351797 1102 46-47 0.4218116810198609 1102 48-49 0.5597393933285986 1102 50-51 0.5547832208874652 1102 52-53 0.6830270901909685 1102 54-55 0.6740515278636323 1102 56-57 0.6812055197144886 1102 58-59 0.6302548748988386 1102 60-61 0.5391733528863867 1102 62-63 0.5364021337503857 1102 64-65 0.6366036603821925 1102 66-67 0.5106966484461637 1102 68-69 0.42052181321967197 1102 70-71 0.5712501079945085 1102 72-73 0.564890897127853 1102 74-75 0.4588228458299568 1102 76-77 0.48144725647593845 1102 78-79 0.48329556788982586 1102 80-81 0.4182322347618026 1102 82-83 0.22610181335193147 1102 84-85 0.2754358639294594 1102 86-87 0.21669938663529464 1102 88-89 0.1423363396240589 1102 90-91 0.4688683874503603 1102 92-93 0.4693960443166745 1102 94-95 0.41481605317490633 1102 96-97 0.3749099240498097 1102 98-99 0.41685363589909485 1102 100-101 0.4807977379303843 1103 1 0.30493860678415174 1103 2 0.35126507160579123 1103 3 0.3476192432891949 1103 4 0.2945711264971962 1103 5 0.15170538096862174 1103 6 0.1267136188335698 1103 7 0.09968277199911313 1103 8 0.23644675788891334 1103 9 0.2800433865368106 1103 10-11 0.4027030918980756 1103 12-13 0.2430851145855044 1103 14-15 0.43481615659319317 1103 16-17 0.4343711427742889 1103 18-19 0.35020572352075874 1103 20-21 0.5125739508815386 1103 22-23 0.5290186793246647 1103 24-25 0.43786909946114605 1103 26-27 0.5413534036969736 1103 28-29 0.452975282482619 1103 30-31 0.4240276434750072 1103 32-33 0.45103642985024095 1103 34-35 0.5056948011544904 1103 36-37 0.41022529725670864 1103 38-39 0.35549907579837026 1103 40-41 0.3731901950727945 1103 42-43 0.3319656129085615 1103 44-45 0.4054210701230261 1103 46-47 0.3113899067266104 1103 48-49 0.4149579783634394 1103 50-51 0.41787472389625435 1103 52-53 0.35641813435895386 1103 54-55 0.42298319316567046 1103 56-57 0.3789590582159761 1103 58-59 0.4080074336691055 1103 60-61 0.47399012370780724 1103 62-63 0.6257751601945785 1103 64-65 0.6146701996838004 1103 66-67 0.5845929540041226 1103 68-69 0.5864584246092832 1103 70-71 0.48362621281641793 1103 72-73 0.4689967485337405 1103 74-75 0.43910202257580266 1103 76-77 0.6299721000494145 1103 78-79 0.4812807428457191 1103 80-81 0.4359856644042708 1103 82-83 0.5477936847707596 1103 84-85 0.30913543012624345 1103 86-87 0.3955073336551642 1103 88-89 0.35474689055180875 1103 90-91 0.434745437401876 1103 92-93 0.5665984451247219 1103 94-95 0.5890722933241328 1103 96-97 0.7318118583969344 1103 98-99 0.896269171792639 1103 100-101 0.9801923262372121 1104 1 0.31895577756446514 1104 2 0.2864810392731236 1104 3 0.25344050436805077 1104 4 0.32102913932966004 1104 5 0.2646904166359576 1104 6 0.27648865003405376 1104 7 0.21494613743079327 1104 8 0.31748789016707235 1104 9 0.28304291763693357 1104 10-11 0.4674457087084818 1104 12-13 0.4354209421065178 1104 14-15 0.568885660090892 1104 16-17 0.5522440034681821 1104 18-19 0.49620821321249764 1104 20-21 0.4679038985091495 1104 22-23 0.4265020912972375 1104 24-25 0.6167277447927333 1104 26-27 0.5147473422734663 1104 28-29 0.5079304275678496 1104 30-31 0.5489069473768708 1104 32-33 0.5241172924673307 1104 34-35 0.5762189296632059 1104 36-37 0.5354593306604656 1104 38-39 0.662673164597372 1104 40-41 0.5619841227003661 1104 42-43 0.5754456434068871 1104 44-45 0.550425461596511 1104 46-47 0.7614387405464385 1104 48-49 0.7512622539210625 1104 50-51 0.44153314263257215 1104 52-53 0.4626950455226648 1104 54-55 0.5518954327219419 1104 56-57 0.5975666873994854 1104 58-59 0.40347909854929753 1104 60-61 0.5291823369549036 1104 62-63 0.6195568020106066 1104 64-65 0.6415912412286531 1104 66-67 0.43079657080105704 1104 68-69 0.41328862176144554 1104 70-71 0.47371727142731146 1104 72-73 0.49103250393461195 1104 74-75 0.47659544726659675 1104 76-77 0.4213909942549634 1104 78-79 0.4829841743835601 1104 80-81 0.4252971273798245 1104 82-83 0.34021289871684246 1104 84-85 0.49113562530284227 1104 86-87 0.42586435608539475 1104 88-89 0.5682551740529611 1104 90-91 0.5650032053908518 1104 92-93 0.5555406593635652 1104 94-95 0.5554520927048046 1104 96-97 0.4823971898440931 1104 98-99 0.577454226977629 1104 100-101 0.7994016705945874 1105 1 0.1720273880106582 1105 2 0.21269151887530668 1105 3 0.19905156138946012 1105 4 0.22090866085801508 1105 5 0.22457226673981268 1105 6 0.2268798675921886 1105 7 0.23840721056662773 1105 8 0.28524049420630604 1105 9 0.3016941324615203 1105 10-11 0.3195471637307037 1105 12-13 0.28601076353177035 1105 14-15 0.3206847377725097 1105 16-17 0.22879879164374728 1105 18-19 0.2273887672447259 1105 20-21 0.315595234014701 1105 22-23 0.4057024280970012 1105 24-25 0.3992642722117097 1105 26-27 0.4380843934531953 1105 28-29 0.2826018537528583 1105 30-31 -0.012480967991216119 1105 32-33 0.03831291305801443 1105 34-35 0.18857117901652032 1105 36-37 0.12015687738806236 1105 38-39 0.09014849941447522 1105 40-41 0.24424864550267955 1105 42-43 0.25727575188813745 1105 44-45 0.29002769702898945 1105 46-47 0.20021585031177125 1105 48-49 0.2930257617681846 1105 50-51 0.18603314853837816 1105 52-53 0.14504216526037794 1105 54-55 0.24423560048062143 1105 56-57 0.4955938102419637 1105 58-59 0.2873481448626407 1105 60-61 0.3753080429098148 1105 62-63 0.38156094620005376 1105 64-65 0.3259967879629855 1105 66-67 0.2929951004237381 1105 68-69 0.34616861406703237 1105 70-71 0.2863841315794815 1105 72-73 0.21536451492954 1105 74-75 0.16761313600354555 1105 76-77 0.400458281650117 1105 78-79 0.2524623709862013 1105 80-81 0.3255962143419957 1105 82-83 0.398110819366412 1105 84-85 0.1989023526587097 1105 86-87 0.17796699226909496 1105 88-89 0.22722778291808154 1105 90-91 0.12719895084717692 1105 92-93 0.1619075416203266 1105 94-95 0.1335218892244292 1105 96-97 0.22336636659140652 1105 98-99 0.09342757997448814 1105 100-101 0.08195110642123993 1106 1 0.2629518549857046 1106 2 0.3153298833299125 1106 3 0.2774159418689308 1106 4 0.2504151016990761 1106 5 0.24458885696364518 1106 6 0.2936780925325735 1106 7 0.3514725846064337 1106 8 0.4482606696570244 1106 9 0.37212239541049996 1106 10-11 0.47134981756005345 1106 12-13 0.4909135048607354 1106 14-15 0.5669081370635567 1106 16-17 0.573164533977085 1106 18-19 0.5516562990917961 1106 20-21 0.547330320754547 1106 22-23 0.296733313069538 1106 24-25 0.5021394954671337 1106 26-27 0.2956294918068778 1106 28-29 0.38323441499837685 1106 30-31 0.47413693091960596 1106 32-33 0.557840371327238 1106 34-35 0.623522777948196 1106 36-37 0.5361205204044452 1106 38-39 0.5480647888646857 1106 40-41 0.45634980543695747 1106 42-43 0.4503873555098181 1106 44-45 0.5032557893587679 1106 46-47 0.48629385940405 1106 48-49 0.5438929244120203 1106 50-51 0.661458037175997 1106 52-53 0.6850515082113731 1106 54-55 0.735653090902666 1106 56-57 0.6372581156206749 1106 58-59 0.8326478116767646 1106 60-61 0.8815297137717693 1106 62-63 0.8576030828079411 1106 64-65 0.8215263729075275 1106 66-67 0.9431316564293937 1106 68-69 0.8465661864496283 1106 70-71 0.7416170601414507 1106 72-73 0.8152704957995667 1106 74-75 0.7232804292398001 1106 76-77 0.8390466571053423 1106 78-79 0.7934853550499525 1106 80-81 0.8612240064035532 1106 82-83 0.8035525239681114 1106 84-85 0.7504498863116993 1106 86-87 0.8352435102627851 1106 88-89 0.8825804548908813 1106 90-91 0.9410725760637781 1106 92-93 0.9124567305895148 1106 94-95 0.9878622340948873 1106 96-97 0.954304287011567 1106 98-99 1.1320844187417052 1106 100-101 0.9976252459886901 1107 1 0.3326527819941383 1107 2 0.3337830128606747 1107 3 0.31597562234252763 1107 4 0.22212961752668292 1107 5 0.22351806683049347 1107 6 0.31101608243829304 1107 7 0.2901554653168006 1107 8 0.3747611362165273 1107 9 0.40525371234015495 1107 10-11 0.4713726714389921 1107 12-13 0.4209366294203747 1107 14-15 0.36316750816694565 1107 16-17 0.44382965155056553 1107 18-19 0.5591545595226037 1107 20-21 0.6228178985367521 1107 22-23 0.5409424075842679 1107 24-25 0.5192072909317247 1107 26-27 0.44751110352724766 1107 28-29 0.5084401201693964 1107 30-31 0.5729791469289509 1107 32-33 0.5739332300411562 1107 34-35 0.5733581084566168 1107 36-37 0.6573003770279655 1107 38-39 0.4037664507631078 1107 40-41 0.7181460943884304 1107 42-43 0.6793725249632203 1107 44-45 0.8105418328839065 1107 46-47 0.7957763065714687 1107 48-49 0.8032008959908694 1107 50-51 0.6507745781490328 1107 52-53 0.6869701804950168 1107 54-55 0.7867942369452763 1107 56-57 0.9011204924792011 1107 58-59 0.936121260899256 1107 60-61 0.9986720517819307 1107 62-63 0.953376395668954 1107 64-65 0.9221678051098792 1107 66-67 0.8979076389800511 1107 68-69 0.8608967031369374 1107 70-71 0.8105388481705873 1107 72-73 0.8098114983327775 1107 74-75 0.8172004708111658 1107 76-77 0.8755385477873006 1107 78-79 0.9010002939498634 1107 80-81 0.8685998932272554 1107 82-83 0.8294531815601118 1107 84-85 0.7565106215485393 1107 86-87 0.7234749944471695 1107 88-89 0.7513955428596724 1107 90-91 0.8055121187203795 1107 92-93 0.7790816551595547 1107 94-95 0.7360034114197518 1107 96-97 0.8798320049577448 1107 98-99 0.8492880894312478 1107 100-101 0.844769913149495 1108 1 0.08905603759497538 1108 2 0.11503278239509385 1108 3 0.13755390191979444 1108 4 0.03534556385281462 1108 5 0.06011260164594745 1108 6 -0.10561774455364059 1108 7 0.009221533825758854 1108 8 -0.0057544461423262305 1108 9 0.003018733324942957 1108 10-11 -0.00589436433600099 1108 12-13 0.09714083009841801 1108 14-15 0.16655816583794802 1108 16-17 0.1339768192528723 1108 18-19 0.0975006647906227 1108 20-21 0.11126640266684973 1108 22-23 0.238434493195939 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76-77 -2.7104764327036293 2316 78-79 -2.9826373581590886 2316 80-81 -3.022381026903844 2316 82-83 -2.7990209445069283 2316 84-85 -2.923506409004986 2316 86-87 -3.5020857652207233 2316 88-89 -3.394035461206226 2316 90-91 -3.090979969828336 2316 92-93 -3.1621943182652466 2316 94-95 -3.501272130412925 2316 96-97 -3.809790192028494 2316 98-99 -3.900502652648669 2316 100-101 -3.97641988531581 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3901.0 3 1482.0 4 1444.0 5 1671.0 6 1772.0 7 2151.0 8 2568.0 9 3307.0 10 4044.0 11 5114.0 12 6519.0 13 7975.0 14 8904.0 15 10986.0 16 13896.0 17 18327.0 18 15811.0 19 10975.0 20 10805.0 21 11352.0 22 12670.0 23 14308.0 24 16836.0 25 19118.0 26 22789.0 27 27205.0 28 32337.0 29 40205.0 30 49880.0 31 62748.0 32 80426.0 33 107218.0 34 146958.0 35 213163.0 36 329234.0 37 499994.0 38 460162.0 39 79006.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.13829747503726 18.764577865749526 14.31512465242294 22.782000006790273 2 15.19955591996374 20.346623480901673 40.70780348439864 23.746017114735952 3 19.425411335793257 25.018076031352155 29.50110153468052 26.055411098174062 4 12.732710480279291 16.713690459517522 37.6964877301004 32.85711133010278 5 13.960255455164816 37.521685885231946 33.82183084691155 14.696227812691681 6 31.34422318536662 36.16582686323868 18.177831736504068 14.312118214890626 7 28.40808370320815 32.88105029731483 20.782238537842694 17.92862746163433 8 26.178755904816565 34.8079809687465 19.945162889734874 19.068100236702065 9 26.5179872972911 17.161774032375693 20.28137493537002 36.038863734963186 10-11 24.694359349687375 26.96304972439487 27.48791786525745 20.854673060660296 12-13 25.9983417927657 24.216809110036017 27.84005135093371 21.944797746264577 14-15 22.91793043403697 25.407826308803124 26.3081875994653 25.36605565769461 16-17 22.228954451130456 28.771895719731393 26.684838732816722 22.31431109632143 18-19 22.16203919451511 27.559136827683854 28.907758687279987 21.371065290521045 20-21 23.20905436780292 26.551386041536677 28.661454420434723 21.57810517022568 22-23 22.76408446968813 26.565602344069916 28.525769280547912 22.144543905694043 24-25 22.49535619215394 26.741968906641006 28.681973200413086 22.08070170079197 26-27 22.405317506229927 26.830130809923507 28.62849943539787 22.136052248448696 28-29 22.19016021738803 27.013271506134245 28.726587541259324 22.0699807352184 30-31 22.41196984035362 26.783137225203074 28.650730666924623 22.154162267518686 32-33 22.207724630705616 26.927196234859284 28.52441552562655 22.34066360880855 34-35 22.60329169867171 26.878825888541634 28.353974596641013 22.163907816145645 36-37 22.55842875691164 26.719973629344228 28.41062708579436 22.310970527949777 38-39 22.440924420889942 26.903997795583656 28.552876612716588 22.10220117080981 40-41 22.662972024604738 26.734679418456608 28.441072042974714 22.16127651396394 42-43 22.382622776742043 26.843297713408266 28.26738764896404 22.50669186088566 44-45 22.580374890152743 26.997470145833034 27.94380571772071 22.47834924629351 46-47 22.549053088674714 27.089726378548594 27.737651590339706 22.623568942436982 48-49 22.703687928941168 27.13727135510835 27.609302842539936 22.549737873410546 50-51 22.644164940537365 27.334973023595204 27.510248064136704 22.510613971730724 52-53 22.854164103564866 27.343063928354766 27.368412121314968 22.4343598467654 54-55 22.615996807943993 27.123481954425806 27.51758886921077 22.74293236841943 56-57 22.61124355549125 27.538591737068923 27.33613110741912 22.514033600020706 58-59 22.826540034632046 27.220563026356288 27.351212768588738 22.601684170422924 60-61 22.77080792831691 27.092864192313538 27.738289507293306 22.398038372076247 62-63 22.709735613766863 27.185605801057683 27.674458022965702 22.430200562209755 64-65 22.61638234783756 27.24878735463211 27.557183022583015 22.577647274947317 66-67 22.457691300829946 27.4586183648627 27.33638168393014 22.74730865037721 68-69 22.413599229079423 27.365686141803046 27.539427571952373 22.681287057165154 70-71 22.53577779390107 27.277382969307617 27.38061445860324 22.80622477818807 72-73 22.621372384585733 27.19617153014689 27.344122084028022 22.838334001239357 74-75 22.44298835348812 27.472364514734327 27.297830190640614 22.786816941136937 76-77 22.603588811489697 27.24404677671246 27.270185181688433 22.882179230109408 78-79 22.551739137353923 27.30313559277079 27.30317803016238 22.841947239712912 80-81 22.51167920412096 27.55070041276635 27.197852443774533 22.739767939338158 82-83 22.497920697966528 27.555589503343857 27.194257731608783 22.75223206708083 84-85 22.616293155791734 27.304467407026788 27.07820539965317 23.001034037528306 86-87 22.651373293328362 27.25531854405321 27.244965114458434 22.848343048159997 88-89 22.675684477054332 27.066202622816572 27.212777400032035 23.045335500097057 90-91 22.530528494428275 27.130513516844797 27.438954148864873 22.900003839862055 92-93 22.611424020002026 27.122361780577712 27.403979782403255 22.862234417017 94-95 22.46080544006511 27.127555414022737 27.36175639491305 23.049882750999103 96-97 22.383715563869522 27.10233514166362 27.38441684265829 23.12953245180857 98-99 22.31307528601419 27.116997353992062 27.23575786438212 23.334169495611633 100-101 22.365064258753335 27.066909246502657 27.346624568934047 23.22140192580996 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 265.0 1 211.5 2 176.5 3 201.0 4 204.5 5 230.5 6 345.0 7 669.5 8 1167.5 9 1472.5 10 1447.5 11 1387.5 12 1466.5 13 1675.0 14 1872.0 15 2056.5 16 2225.5 17 2359.5 18 2491.5 19 2656.5 20 3016.5 21 3498.5 22 4093.0 23 5041.0 24 6222.0 25 7789.0 26 10198.0 27 13350.0 28 16491.5 29 20142.5 30 24577.0 31 29233.0 32 33773.5 33 38471.5 34 44706.5 35 49688.5 36 54701.0 37 61418.0 38 66377.0 39 69663.0 40 72716.5 41 76395.5 42 79962.5 43 83749.5 44 87530.0 45 89831.5 46 90942.5 47 93908.0 48 103125.0 49 103983.0 50 95988.5 51 91239.5 52 86224.0 53 82285.5 54 78295.5 55 72284.0 56 66067.0 57 58960.0 58 52408.0 59 46390.5 60 38620.0 61 30666.5 62 23687.5 63 17769.5 64 13190.5 65 10052.5 66 7257.0 67 5305.5 68 3816.0 69 2517.0 70 1731.5 71 1161.0 72 770.0 73 525.5 74 361.5 75 249.0 76 165.0 77 78.0 78 33.0 79 24.5 80 22.5 81 14.0 82 12.5 83 11.5 84 9.0 85 6.0 86 6.5 87 7.0 88 4.5 89 3.5 90 2.5 91 1.5 92 4.5 93 4.5 94 1.0 95 1.5 96 1.5 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04123389460073542 2 0.0403854605554528 3 0.024265213695082985 4 0.04369435333205503 5 0.0631659146712912 6 0.06677175936374234 7 0.06499004786864884 8 0.06702628957732712 9 0.06643238574562929 10-11 0.0738137619395881 12-13 0.07330470151241854 14-15 0.0663051206388369 16-17 0.05459673081393672 18-19 0.048275897176581185 20-21 0.05489368272978564 22-23 0.029652769882627633 24-25 0.016205090264898078 26-27 0.013341625362069228 28-29 0.016650518138671456 30-31 0.012450769614522477 32-33 0.00795406917452458 34-35 0.00990546747867461 36-37 0.005026971718299534 38-39 0.00837828619716589 40-41 0.007508641300751204 42-43 0.0054936104432049765 44-45 0.0014211270258483917 46-47 0.0028634649028288486 48-49 0.0011029642588674085 50-51 0.005578453847733238 52-53 0.004836074058110945 54-55 0.007741960663203925 56-57 0.022462291348857414 58-59 0.009269141944712645 60-61 0.01976851325508509 62-63 0.01419005940735185 64-65 0.01047816045924038 66-67 0.016162668562633948 68-69 0.026343877106025405 70-71 0.018877657507538337 72-73 0.017626217290746468 74-75 0.01870797069848181 76-77 0.02538938880508246 78-79 0.036970513523190245 80-81 0.031604168186777665 82-83 0.03071331243923091 84-85 0.02133811623885794 86-87 0.024477322206403643 88-89 0.019789724106217155 90-91 0.018177699420180174 92-93 0.03067089073696678 94-95 0.023225881989611775 96-97 0.03709777862998264 98-99 0.054384622302616065 100-101 0.06346286658714011 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2357284.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.424416876290366 #Duplication Level Percentage of deduplicated Percentage of total 1 75.00893521824995 42.323354302011936 2 14.09737940218668 15.908728245044207 3 4.72842068883711 8.003951403402633 4 2.1034420118857287 4.747419558149732 5 1.1327382979123213 3.1957048966572206 6 0.65148570418335 2.2055820577070957 7 0.45269352556500875 1.7880077742574367 8 0.3191564706190815 1.4406574197581257 9 0.22718421865581495 1.1536863355035005 >10 1.1719531701849253 11.637633952387771 >50 0.07059357698604682 2.729265096064765 >100 0.0342080717973731 3.488784124069376 >500 0.0012064286243651428 0.4346824669941988 >1k 5.27812523158737E-4 0.6480457411417587 >5k 7.540178902267671E-5 0.2944966268502662 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6922 0.29364302307231543 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4927 0.2090117270553739 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3112 0.13201633744597596 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.242170226413109E-5 0.0 0.0 0.0 0.0 3 4.242170226413109E-5 0.0 0.0 0.0 0.0 4 4.242170226413109E-5 0.0 0.0 0.0 0.0 5 4.242170226413109E-5 0.0 0.0 0.0 0.0 6 4.242170226413109E-5 0.0 0.0 0.0 0.0 7 4.242170226413109E-5 0.0 0.0 4.242170226413109E-5 0.0 8 4.242170226413109E-5 0.0 0.0 4.242170226413109E-5 0.0 9 4.242170226413109E-5 0.0 0.0 8.484340452826218E-5 0.0 10-11 4.242170226413109E-5 0.0 0.0 1.0605425566032773E-4 0.0 12-13 4.242170226413109E-5 0.0 0.0 1.6968680905652436E-4 0.0 14-15 4.242170226413109E-5 0.0 0.0 1.6968680905652436E-4 0.0 16-17 4.242170226413109E-5 0.0 0.0 2.1210851132065546E-4 0.0 18-19 6.363255339619664E-5 0.0 0.0 2.5453021358478657E-4 0.0 20-21 8.484340452826218E-5 0.0 0.0 3.393736181130487E-4 0.0 22-23 8.484340452826218E-5 0.0 0.0 4.030061715092454E-4 0.0 24-25 1.6968680905652436E-4 0.0 0.0 5.939038316978353E-4 0.0 26-27 1.6968680905652436E-4 0.0 0.0 0.0010393317054712117 0.0 28-29 1.6968680905652436E-4 0.0 0.0 0.0016968680905652437 0.0 30-31 1.6968680905652436E-4 0.0 0.0 0.0029695191584891765 0.0 32-33 1.6968680905652436E-4 0.0 0.0 0.004390646184337568 0.0 34-35 1.6968680905652436E-4 0.0 0.0 0.006172357679431074 0.0 36-37 2.1210851132065546E-4 0.0 0.0 0.0081873885369773 0.0 38-39 2.1210851132065546E-4 0.0 0.0 0.010902377481881692 0.0 40-41 2.1210851132065546E-4 0.0 0.0 0.01529302366621926 0.0 42-43 2.1210851132065546E-4 0.0 0.0 0.020213941128858465 0.0 44-45 2.1210851132065546E-4 0.0 0.0 0.030034565203004814 0.0 46-47 2.1210851132065546E-4 0.0 0.0 0.05143631399525895 0.0 48-49 2.1210851132065546E-4 0.0 0.0 0.06677175936374234 0.0 50-51 2.1210851132065546E-4 0.0 0.0 0.08367680771599859 0.0 52-53 2.33319362452721E-4 0.0 0.0 0.1074965935373082 0.0 54-55 2.5453021358478657E-4 0.0 0.0 0.1390795508729538 0.0 56-57 2.5453021358478657E-4 0.0 0.0 0.17151094225388203 0.0 58-59 2.5453021358478657E-4 0.0 0.0 0.21081464940159947 0.0 60-61 2.5453021358478657E-4 0.0 0.0 0.2639266206362916 0.0 62-63 2.5453021358478657E-4 0.0 0.0 0.3247381308319235 0.0 64-65 2.5453021358478657E-4 0.0 0.0 0.37114747310888296 0.0 66-67 2.5453021358478657E-4 0.0 0.0 0.45202444847544887 0.0 68-69 2.5453021358478657E-4 0.0 0.0 0.5602422109512473 0.0 70-71 2.9695191584891764E-4 0.0 0.0 0.6265261207389521 0.0 72-73 2.9695191584891764E-4 0.0 0.0 0.7483188279392725 0.0 74-75 2.9695191584891764E-4 0.0 0.0 0.8452736284639442 0.0 76-77 2.9695191584891764E-4 0.0 0.0 0.9212296863678708 0.0 78-79 2.9695191584891764E-4 0.0 0.0 1.017420896251788 0.0 80-81 2.9695191584891764E-4 0.0 0.0 1.122605507015701 0.0 82-83 2.9695191584891764E-4 2.1210851132065545E-5 0.0 1.2599669789469576 0.0 84-85 3.393736181130487E-4 4.242170226413109E-5 0.0 1.4702089353679915 0.0 86-87 3.8179532037717985E-4 4.242170226413109E-5 0.0 1.7591643603401201 0.0 88-89 3.8179532037717985E-4 4.242170226413109E-5 0.0 2.094571549291473 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12535 0.0 28.85132 1 GGTATCA 8755 0.0 28.009083 1 GTACATG 11090 0.0 25.925621 1 TACATGG 11325 0.0 24.758204 2 TATCAAC 14865 0.0 24.105257 2 ATCAACG 14810 0.0 23.969646 3 TCAACGC 14890 0.0 23.969038 4 ACATGGG 11415 0.0 23.646591 3 CAACGCA 15105 0.0 23.565945 5 AACGCAG 15570 0.0 22.830166 6 GAGTACT 6575 0.0 22.694027 12-13 ACGCAGA 17040 0.0 20.610106 7 GTACTTT 7190 0.0 20.586124 14-15 CGCAGAG 17035 0.0 20.366806 8 CATGGGG 4800 0.0 20.197344 4 AGTACTT 6965 0.0 19.95641 12-13 CATGGGA 6940 0.0 19.858383 4 GCAGAGT 17715 0.0 18.914295 9 AGAGTAC 14225 0.0 18.174253 10-11 ATGGGAG 3030 0.0 17.096518 5 >>END_MODULE