FastQCFastQC Report
Thu 26 May 2016
SRR936842_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936842_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357284
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80770.34264008918738686No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60460.2564816118889366No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39460.1673960371342613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90550.029.5422211
GTATCAA129350.029.2762241
GTACATG109000.024.8032721
TATCAAC155850.024.2000962
ATCAACG155900.024.0079963
TCAACGC156250.023.982584
TACATGG111300.023.8145522
CAACGCA157850.023.70945
ACATGGG109300.023.2492733
AACGCAG162100.023.1160966
GAGTACT80050.021.38885312-13
ACGCAGA179600.020.6257047
CGCAGAG180150.020.4309188
GTACTTT84900.020.14008714-15
TACACCG2851.9699655E-919.9974825
AGTACTT81750.019.69497712-13
CATGGGA66050.019.4146634
GCAGAGT189750.019.021839
CATGGGG48900.018.9393314
AGAGTAC154900.018.257910-11
Produced by FastQC (version 0.11.5)