##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936842_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2357284 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28606862813305 33.0 31.0 34.0 31.0 34.0 2 32.3523364176739 33.0 31.0 34.0 31.0 34.0 3 32.49832349432652 34.0 31.0 34.0 31.0 34.0 4 36.14638117426666 37.0 35.0 37.0 35.0 37.0 5 36.06617573444693 37.0 35.0 37.0 35.0 37.0 6 36.096903894481954 37.0 35.0 37.0 35.0 37.0 7 36.05201324914605 37.0 35.0 37.0 35.0 37.0 8 36.03556296144207 37.0 35.0 37.0 35.0 37.0 9 37.75346924681116 39.0 37.0 39.0 35.0 39.0 10-11 37.653376300861495 39.0 37.0 39.0 35.0 39.0 12-13 37.5831072963631 39.0 37.0 39.0 35.0 39.0 14-15 38.85307476740181 40.0 38.0 41.0 35.0 41.0 16-17 38.77617864457571 40.0 38.0 41.0 35.0 41.0 18-19 38.799005974672546 40.0 38.0 41.0 35.0 41.0 20-21 38.88315387539219 40.0 38.0 41.0 35.0 41.0 22-23 38.82554690058559 40.0 38.0 41.0 35.0 41.0 24-25 38.74317752973337 40.0 38.0 41.0 35.0 41.0 26-27 38.58746294464307 40.0 38.0 41.0 34.0 41.0 28-29 38.44506368345944 40.0 38.0 41.0 34.0 41.0 30-31 38.43575763463376 40.0 38.0 41.0 34.0 41.0 32-33 38.381126542240985 40.0 38.0 41.0 34.0 41.0 34-35 38.285083171989456 40.0 38.0 41.0 34.0 41.0 36-37 38.13778102256665 40.0 38.0 41.0 34.0 41.0 38-39 38.01293077117565 40.0 38.0 41.0 33.0 41.0 40-41 37.80859412781829 40.0 37.0 41.0 33.0 41.0 42-43 37.61516134670239 40.0 37.0 41.0 33.0 41.0 44-45 37.401829181379924 40.0 37.0 41.0 32.5 41.0 46-47 37.091168692444356 40.0 37.0 41.0 31.5 41.0 48-49 36.7759444767792 40.0 36.0 41.0 31.0 41.0 50-51 36.55670318043986 39.0 36.0 41.0 31.0 41.0 52-53 36.347219511946804 39.0 36.0 41.0 30.0 41.0 54-55 36.09574959996335 39.0 35.0 40.0 30.0 41.0 56-57 35.858576650076955 39.0 35.0 40.0 29.0 41.0 58-59 35.52662513299204 38.0 35.0 40.0 28.0 41.0 60-61 35.20824156105077 38.0 34.0 40.0 28.0 41.0 62-63 34.88356112373393 37.5 34.0 40.0 28.0 41.0 64-65 34.48237802487948 37.0 34.0 39.5 26.5 41.0 66-67 34.05775820817517 36.5 33.5 39.0 26.0 40.5 68-69 33.63716505944977 36.0 33.0 39.0 26.0 40.0 70-71 33.248396883871436 35.0 33.0 38.5 26.0 40.0 72-73 32.85710101116369 35.0 32.0 38.0 25.0 40.0 74-75 32.39127996456939 35.0 32.0 37.0 24.5 39.0 76-77 30.90412695288306 33.5 30.0 35.5 22.0 38.0 78-79 31.61333191079225 34.5 31.0 36.0 23.0 38.5 80-81 31.330944213764653 34.5 31.0 36.0 21.0 37.5 82-83 31.1423629906282 34.0 31.0 35.0 21.0 37.0 84-85 30.831617021962565 34.0 31.0 35.0 20.0 36.5 86-87 30.483359875178383 34.0 31.0 35.0 19.5 36.0 88-89 30.133779383392074 34.0 30.5 35.0 18.0 36.0 90-91 29.71777286911547 34.0 30.0 35.0 12.5 35.0 92-93 29.323703465513702 34.0 29.5 35.0 6.0 35.0 94-95 28.884308382019306 34.0 29.0 35.0 2.0 35.0 96-97 28.312899506381072 34.0 29.0 35.0 2.0 35.0 98-99 27.59770142248452 33.0 28.5 35.0 2.0 35.0 100-101 24.41308514375018 30.0 21.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25527440024559667 1101 2 0.22322484324509873 1101 3 0.24762329517746906 1101 4 0.11843723467550404 1101 5 0.21058466169902346 1101 6 0.18944993618939776 1101 7 0.22433495227652145 1101 8 0.20031038107715204 1101 9 0.19259143426735648 1101 10-11 0.1856100280000561 1101 12-13 0.2471656328396321 1101 14-15 0.2558438191450634 1101 16-17 0.24235765697801526 1101 18-19 0.28775087580441294 1101 20-21 0.2119615525397336 1101 22-23 0.28875063657119426 1101 24-25 0.21915700204095145 1101 26-27 0.24517123951214614 1101 28-29 0.32508930093481325 1101 30-31 0.22296931824160282 1101 32-33 0.3967468715818896 1101 34-35 0.42095693962905045 1101 36-37 0.42328185909369154 1101 38-39 0.3478567479796766 1101 40-41 0.45409733655359474 1101 42-43 0.4675412733262547 1101 44-45 0.47533695347451754 1101 46-47 0.5285758548183139 1101 48-49 0.5161196722247965 1101 50-51 0.44631721873501107 1101 52-53 0.5111910693806578 1101 54-55 0.5235653715639899 1101 56-57 0.3933726911051565 1101 58-59 0.47657911196780134 1101 60-61 0.39482616380912106 1101 62-63 0.32395634526810113 1101 64-65 0.40028959582559054 1101 66-67 0.5025535584375902 1101 68-69 0.5587303386106797 1101 70-71 0.603739302734148 1101 72-73 0.6768982189702442 1101 74-75 0.6418407348426101 1101 76-77 0.4492908596033729 1101 78-79 0.6413971701832573 1101 80-81 0.9497254629333547 1101 82-83 0.7763003397052692 1101 84-85 0.6629283940919919 1101 86-87 0.48255572309062345 1101 88-89 0.46433798324803277 1101 90-91 0.4193766155118581 1101 92-93 0.5741444857224565 1101 94-95 0.5136526679643616 1101 96-97 0.6342219504141511 1101 98-99 0.7980631705668664 1101 100-101 1.023296921247951 1102 1 0.21347717061610894 1102 2 0.23349811603391402 1102 3 0.20415001985806214 1102 4 0.11724200231929416 1102 5 0.15685108289783045 1102 6 0.21157367603142774 1102 7 0.19727275337796613 1102 8 0.18695579073327195 1102 9 0.2616227851946604 1102 10-11 0.25171553489435183 1102 12-13 0.24011507468493676 1102 14-15 0.2500848932567479 1102 16-17 0.31075034861534334 1102 18-19 0.33295602809887015 1102 20-21 0.1849998657706422 1102 22-23 0.24019173219294743 1102 24-25 0.16565022795046502 1102 26-27 0.2710833613856778 1102 28-29 0.2373770076133752 1102 30-31 0.2950371477173519 1102 32-33 0.2222037233300611 1102 34-35 0.2374250335521353 1102 36-37 0.22750550372202838 1102 38-39 0.28106869176247784 1102 40-41 0.36199509096553584 1102 42-43 0.2688805915202934 1102 44-45 0.30722832129355737 1102 46-47 0.28445450955322116 1102 48-49 0.42556789147567287 1102 50-51 0.4303383998276544 1102 52-53 0.4091305311860509 1102 54-55 0.40289174259372373 1102 56-57 0.44077914602416257 1102 58-59 0.33982227698482603 1102 60-61 0.3459517256806208 1102 62-63 0.2921899256509164 1102 64-65 0.36850079624566945 1102 66-67 0.1621129657718896 1102 68-69 0.1041463212254925 1102 70-71 0.20249369569150844 1102 72-73 0.250088842477588 1102 74-75 0.3152039133983422 1102 76-77 0.25704682153055103 1102 78-79 0.4135077825559783 1102 80-81 0.5025711977711218 1102 82-83 0.44219620249632996 1102 84-85 0.1383802637283118 1102 86-87 0.13343692423825004 1102 88-89 0.21881318498402536 1102 90-91 0.2502498136480753 1102 92-93 0.36485972718881143 1102 94-95 0.46921887311349053 1102 96-97 0.4773788220907704 1102 98-99 0.5900917125320646 1102 100-101 1.1725570380982475 1103 1 0.1936048000475452 1103 2 0.10546326248429239 1103 3 0.12494730335884441 1103 4 0.10428482357200153 1103 5 0.2260832457633768 1103 6 0.20181213172619294 1103 7 0.17182648969185976 1103 8 0.09501625725640395 1103 9 0.1822432505358904 1103 10-11 0.13363342875214101 1103 12-13 0.14555102786622598 1103 14-15 0.31225459359117025 1103 16-17 0.23583932142935993 1103 18-19 0.2782318185514683 1103 20-21 0.23243759646430107 1103 22-23 0.2280412765790487 1103 24-25 0.26111632730609813 1103 26-27 0.2985383851130621 1103 28-29 0.27251778954774863 1103 30-31 0.36365604548861796 1103 32-33 0.34126361678817574 1103 34-35 0.3977809870133484 1103 36-37 0.39895590249595614 1103 38-39 0.32520231777107966 1103 40-41 0.2862645885889066 1103 42-43 0.32649469716175616 1103 44-45 0.4281657996675463 1103 46-47 0.4874881773170259 1103 48-49 0.5306519967361325 1103 50-51 0.45554688430008383 1103 52-53 0.45905055868073674 1103 54-55 0.5818529475698284 1103 56-57 0.5076595651625126 1103 58-59 0.5269504748158909 1103 60-61 0.5983508619620181 1103 62-63 0.5542829581290079 1103 64-65 0.42987831390221487 1103 66-67 0.4438952523868167 1103 68-69 0.4602130735797587 1103 70-71 0.4740398904076457 1103 72-73 0.500709999851189 1103 74-75 0.4170172714893212 1103 76-77 0.5299105992341993 1103 78-79 0.571668127234755 1103 80-81 0.8485674794578522 1103 82-83 0.6889882436574801 1103 84-85 0.668816936156734 1103 86-87 0.6889677014061597 1103 88-89 0.5832296578427467 1103 90-91 0.4325087472806146 1103 92-93 0.5280248655525206 1103 94-95 0.64866971043379 1103 96-97 0.7849889232752325 1103 98-99 0.9402349787849111 1103 100-101 1.065029980798741 1104 1 0.15034477959367365 1104 2 0.06435859466930793 1104 3 0.06440669591531645 1104 4 0.10099506645578771 1104 5 0.02915941938168487 1104 6 0.08341451573694059 1104 7 0.055665946624870344 1104 8 0.09787123072904791 1104 9 0.17674244404396688 1104 10-11 0.11145430582499927 1104 12-13 0.11499383041176969 1104 14-15 0.15972630290180945 1104 16-17 0.160555422784725 1104 18-19 0.1548852856733376 1104 20-21 0.10406804306168027 1104 22-23 0.12462449599324543 1104 24-25 0.11938471417150254 1104 26-27 0.2031255905729239 1104 28-29 0.16406172372087013 1104 30-31 0.23763464112158061 1104 32-33 0.28274943435200584 1104 34-35 0.2309886192117716 1104 36-37 0.21632582641675668 1104 38-39 0.3551140836670541 1104 40-41 0.36300691660142803 1104 42-43 0.35936235711334774 1104 44-45 0.36617763244721857 1104 46-47 0.32803659182452094 1104 48-49 0.4736623339552821 1104 50-51 0.4080631946372293 1104 52-53 0.32056712845993474 1104 54-55 0.2874021515111167 1104 56-57 0.2279737994870885 1104 58-59 0.2374380434153096 1104 60-61 0.26985666529223806 1104 62-63 0.26588652430696413 1104 64-65 0.2493509086481538 1104 66-67 0.16244305914928958 1104 68-69 0.2973716831289721 1104 70-71 0.25268780848424655 1104 72-73 0.14583665438940585 1104 74-75 0.1791107229970592 1104 76-77 0.23843394544972085 1104 78-79 0.4902118083130169 1104 80-81 0.5513681049750438 1104 82-83 0.5361732179155929 1104 84-85 0.3463130742782923 1104 86-87 0.41584034550163196 1104 88-89 0.28503186778136325 1104 90-91 0.32385250642951746 1104 92-93 0.5386246210263899 1104 94-95 0.4928538841504846 1104 96-97 0.6603973065068658 1104 98-99 0.9261084773730559 1104 100-101 1.0553446197577365 1105 1 0.15238278937031424 1105 2 0.07001332091826384 1105 3 0.07555551045102504 1105 4 0.06747690947268126 1105 5 0.059486078027703115 1105 6 0.07195978037617579 1105 7 0.05290101632183308 1105 8 0.06332748810076083 1105 9 0.11585896899937609 1105 10-11 0.07238612412186285 1105 12-13 0.11675789509217793 1105 14-15 0.19743733005671515 1105 16-17 0.13335766091700663 1105 18-19 0.1769668465613634 1105 20-21 0.1942950285519558 1105 22-23 0.0299117122824768 1105 24-25 0.11369126269952545 1105 26-27 0.18763592320487277 1105 28-29 0.1884167673647852 1105 30-31 0.17534777749342112 1105 32-33 0.17264046376799058 1105 34-35 0.16877195032340353 1105 36-37 0.18374331806847977 1105 38-39 0.21147143226324516 1105 40-41 0.3864486693100915 1105 42-43 0.34755150156433245 1105 44-45 0.38580803014428966 1105 46-47 0.3862561258731887 1105 48-49 0.338165562744571 1105 50-51 0.24932023731259534 1105 52-53 0.22274687761498058 1105 54-55 0.22257730932273034 1105 56-57 0.2264810644739228 1105 58-59 0.30017729189287223 1105 60-61 0.2875713034265388 1105 62-63 0.1583928139918811 1105 64-65 0.19928223254456867 1105 66-67 0.19332874465322902 1105 68-69 0.17862032268404704 1105 70-71 0.16916408316841114 1105 72-73 0.24172131903234373 1105 74-75 0.25869687775959704 1105 76-77 0.17804757065368193 1105 78-79 0.17198800520926127 1105 80-81 0.46738870443137515 1105 82-83 0.2948675788819166 1105 84-85 0.05486932371934827 1105 86-87 -0.040035848526081 1105 88-89 -0.17983979902310665 1105 90-91 -0.18313701666809834 1105 92-93 0.02606060629224416 1105 94-95 -0.1435385449418618 1105 96-97 0.11178432756697276 1105 98-99 0.14690468145751012 1105 100-101 0.3841987918732137 1106 1 0.14353377152571767 1106 2 0.10761827750032182 1106 3 0.10823124334033452 1106 4 0.12702836696292508 1106 5 0.1310664585718584 1106 6 0.134363314322961 1106 7 0.14496159691902477 1106 8 0.1505156169937294 1106 9 0.1601869419563684 1106 10-11 0.1860408655263015 1106 12-13 0.10266942917238708 1106 14-15 0.20565873946429036 1106 16-17 0.17692755094106616 1106 18-19 0.2035495299930048 1106 20-21 0.14535260478225354 1106 22-23 0.17694644562091355 1106 24-25 0.1549730057377161 1106 26-27 0.21301527632765271 1106 28-29 0.27123227390064386 1106 30-31 0.238368054021862 1106 32-33 0.2574735659141112 1106 34-35 0.20088282719623862 1106 36-37 0.2916004938962118 1106 38-39 0.4141060736883375 1106 40-41 0.4335059935199226 1106 42-43 0.46129300134887075 1106 44-45 0.3690188516602362 1106 46-47 0.5185148963031025 1106 48-49 0.5039924981387216 1106 50-51 0.3069098604038629 1106 52-53 0.3632381760021488 1106 54-55 0.4842643369738937 1106 56-57 0.4377201156505848 1106 58-59 0.5054648024260189 1106 60-61 0.3966030866431254 1106 62-63 0.438318895852035 1106 64-65 0.36246910200909355 1106 66-67 0.4091750027390333 1106 68-69 0.46486157230120995 1106 70-71 0.4481172919313181 1106 72-73 0.3502092198602753 1106 74-75 0.5295755767410313 1106 76-77 0.35046730062116893 1106 78-79 0.4241802968198449 1106 80-81 0.7268301219723377 1106 82-83 0.5861394668419742 1106 84-85 0.43450756991301276 1106 86-87 0.5252288588724419 1106 88-89 0.39560924719063806 1106 90-91 0.5598793548909242 1106 92-93 0.4935321099547956 1106 94-95 0.6376133570208324 1106 96-97 0.7507117008635191 1106 98-99 0.731274089542989 1106 100-101 0.6520137407969528 1107 1 0.19235289863397043 1107 2 0.13851963934838096 1107 3 0.10658349487693641 1107 4 0.11363534556505073 1107 5 0.062422482943496505 1107 6 0.055244644178827684 1107 7 0.13071698488306538 1107 8 0.1559060528236813 1107 9 0.18826706538676774 1107 10-11 0.08126806793111285 1107 12-13 0.28144381616571224 1107 14-15 0.19372236920163033 1107 16-17 0.2235276636544583 1107 18-19 0.19403737278295807 1107 20-21 0.22154630259010588 1107 22-23 0.21974430500666386 1107 24-25 0.29591190467672135 1107 26-27 0.26213434033650174 1107 28-29 0.2187397504417845 1107 30-31 0.2651741952643434 1107 32-33 0.2686719665964148 1107 34-35 0.31193444438372353 1107 36-37 0.3995085681392254 1107 38-39 0.39978430955041944 1107 40-41 0.4934663055709976 1107 42-43 0.38127970719886406 1107 44-45 0.33063300654243477 1107 46-47 0.37634788501831196 1107 48-49 0.5109701439655154 1107 50-51 0.5631302891458745 1107 52-53 0.5370993842332439 1107 54-55 0.5840882972723307 1107 56-57 0.6210959933975673 1107 58-59 0.6860431870976456 1107 60-61 0.5892219807066397 1107 62-63 0.6359149109135487 1107 64-65 0.5841967061397142 1107 66-67 0.5244944602560153 1107 68-69 0.5617511593770033 1107 70-71 0.49235494569051497 1107 72-73 0.4918724733028199 1107 74-75 0.6010742611842304 1107 76-77 0.6426249677490752 1107 78-79 0.6123660246210711 1107 80-81 0.7628965415037463 1107 82-83 0.7744970849880204 1107 84-85 0.7793535582711506 1107 86-87 0.7227617421194275 1107 88-89 0.550090447119775 1107 90-91 0.5038107666722738 1107 92-93 0.72528721816143 1107 94-95 0.637635805689154 1107 96-97 0.6048460080854596 1107 98-99 0.7952516596764347 1107 100-101 0.957029146094289 1108 1 0.0033017049745822646 1108 2 -0.009490725269806433 1108 3 -0.04030957262230572 1108 4 -0.037229746058443425 1108 5 -0.08676257474264304 1108 6 -0.04030069268889491 1108 7 -0.042190235366994955 1108 8 -0.03496978691271835 1108 9 -0.04681038868284304 1108 10-11 -0.009607755772904625 1108 12-13 -0.16326208089930816 1108 14-15 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2316 72-73 -1.0481028506266448 2316 74-75 -1.1662087393613838 2316 76-77 -1.1200320915095077 2316 78-79 -0.9751758176227447 2316 80-81 -0.824733023931703 2316 82-83 -0.958783166100563 2316 84-85 -1.2554401943431692 2316 86-87 -1.2859681351592656 2316 88-89 -1.2992507995328815 2316 90-91 -1.3708714982837407 2316 92-93 -1.3906111084178185 2316 94-95 -1.4580018260955114 2316 96-97 -1.672597041704698 2316 98-99 -1.6920697238752105 2316 100-101 -1.9511808629276004 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 6.0 4 18.0 5 45.0 6 83.0 7 192.0 8 524.0 9 973.0 10 1644.0 11 2733.0 12 4125.0 13 5902.0 14 7440.0 15 10154.0 16 13859.0 17 19243.0 18 17300.0 19 10516.0 20 9300.0 21 9542.0 22 10135.0 23 11057.0 24 13093.0 25 15909.0 26 19559.0 27 24806.0 28 31343.0 29 39851.0 30 50308.0 31 64489.0 32 82852.0 33 110023.0 34 153519.0 35 224090.0 36 356104.0 37 559851.0 38 441092.0 39 35602.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25573560137889 19.183819298641648 14.827698579869002 22.73274652011046 2 15.439003767731908 20.700719224850992 40.03875914728179 23.82151786013531 3 19.514893822832004 24.84821075148565 29.817004383867808 25.81989104181454 4 13.089815690351985 17.095529047636624 37.25365176773429 32.561003494277095 5 13.989904817823454 37.560517638993296 33.78059396800562 14.66898357517763 6 31.626057785145957 36.283409211618114 17.71504833528756 14.375484667948369 7 28.532624834343252 32.82362244006238 20.683931168242776 17.959821557351596 8 26.40671484925654 34.63440749371204 19.895659536848143 19.06321812018328 9 26.601950890898923 16.80439591020506 20.20653447487403 36.38711872402199 10-11 24.799281715737266 26.83166304950952 27.391481043438127 20.97757419131509 12-13 26.01889255719787 24.09756594206664 27.95627583091087 21.927265669824617 14-15 22.90292557027494 25.307599763117217 26.40386139302689 25.385613273580955 16-17 22.174057092823777 28.662159502206773 26.89071405906119 22.273069345908258 18-19 22.078049900060307 27.284671480204135 29.247723383724946 21.389555236010615 20-21 23.018912267796455 26.242388916251247 29.23330673457622 21.505392081376076 22-23 22.591932070976597 26.264293992577898 29.10262403681525 22.041149899630252 24-25 22.438834206771794 26.44244618203524 29.217884750243833 21.900834860949132 26-27 22.383076425733243 26.59101842997737 29.10966604416033 21.916239100129054 28-29 22.07412969833502 26.680942774655986 29.32741076371875 21.91751676329025 30-31 22.3122367954265 26.474115352560457 29.18833692901263 22.02531092300041 32-33 22.19756556650658 26.51619696232453 29.138539059918216 22.147698411250673 34-35 22.549890748482664 26.539496104553944 28.933887188289543 21.976725958673846 36-37 22.451169865437365 26.35618048322051 28.99295471372293 22.1996949376192 38-39 22.37109159560477 26.589707691655 29.106550422128883 21.932650290611345 40-41 22.553342867797763 26.446662647540602 28.985521388270065 22.01447309639157 42-43 22.328681838381677 26.550197220981897 28.778375374263803 22.342745566372628 44-45 22.529021425201847 26.62593796771117 28.477543924548538 22.367496682538444 46-47 22.5860075104911 26.855456984366437 28.092774758233794 22.465760746908668 48-49 22.732251564322834 26.956623183794676 27.901665075829886 22.409460176052605 50-51 22.675569577803216 27.079596123631934 27.860923512640916 22.383910785923934 52-53 22.89935368644778 27.11778518293834 27.635748118012142 22.347113012601735 54-55 22.591292502750562 26.924954114857414 27.84272060026018 22.641032782131848 56-57 22.635698015348716 27.25989075053277 27.63220774240322 22.472203491715295 58-59 22.885219043554603 27.04965236114225 27.608330775631618 22.45679781967153 60-61 22.7820449296733 26.91659129744231 28.011453011177267 22.28991076170712 62-63 22.66959461379902 26.99936749215092 27.924776108765897 22.40626178528416 64-65 22.62237036867339 27.056878988701076 27.799371862441014 22.52137878018452 66-67 22.531645730716352 27.266022672847267 27.53491434367482 22.667417252761567 68-69 22.52924260042261 27.133289751716287 27.756724642095516 22.580743005765587 70-71 22.58073685036664 27.056204514263776 27.65358826910799 22.709470366261595 72-73 22.640268661104702 26.941693149595793 27.64278985151387 22.775248337785634 74-75 22.59156867939969 27.165712513599573 27.508520347546607 22.734198459454134 76-77 22.576981710712225 27.067352593617976 27.531865673652145 22.823800022017654 78-79 22.61082729166671 27.01653285091924 27.556978907181882 22.815660950232168 80-81 22.668400019259476 27.280004530642604 27.34724300004051 22.704352450057407 82-83 22.59338874740525 27.22921579882257 27.41015364656581 22.767241807206375 84-85 22.59170707019715 27.06208603968759 27.334966301992015 23.01124058812325 86-87 22.609558288267344 26.959458427580213 27.452016812569042 22.978966471583398 88-89 22.614859427358393 26.842156393188503 27.440925892066648 23.102058287386452 90-91 22.451673388785956 26.83619911613765 27.678509121260102 23.033618373816296 92-93 22.48525416624952 26.807637289820963 27.62519111041728 23.08191743351224 94-95 22.386065549256116 26.796196694856818 27.618950856405437 23.198786899481625 96-97 22.204812760496758 26.6851131072955 27.69252632806949 23.417547804138252 98-99 22.154326172537843 26.727160116744724 27.479679970135074 23.638833740582367 100-101 22.03448538440507 26.821824241242847 27.572144687506828 23.571545686845255 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 353.0 1 264.0 2 196.5 3 245.5 4 235.0 5 219.5 6 356.0 7 759.5 8 1348.5 9 1664.0 10 1573.5 11 1516.0 12 1649.0 13 1852.0 14 2116.5 15 2323.5 16 2459.0 17 2633.0 18 2856.5 19 3093.5 20 3431.0 21 4002.5 22 4633.0 23 5455.5 24 6664.5 25 8341.0 26 10681.0 27 13726.5 28 16880.0 29 20369.0 30 24435.5 31 29078.0 32 33831.5 33 38220.5 34 43905.0 35 48826.0 36 53850.5 37 59962.5 38 65172.5 39 69019.0 40 72078.5 41 75826.0 42 79563.5 43 83275.5 44 86515.5 45 88899.0 46 90638.0 47 94241.5 48 103914.5 49 104630.5 50 96089.0 51 91275.5 52 86584.5 53 82768.0 54 78478.0 55 72203.0 56 65505.0 57 58371.0 58 52413.5 59 46616.0 60 39083.5 61 31297.0 62 24069.5 63 17983.0 64 13118.5 65 9761.5 66 7257.5 67 5461.0 68 3836.0 69 2382.0 70 1648.0 71 1137.0 72 760.5 73 545.0 74 403.5 75 282.0 76 175.5 77 79.5 78 34.0 79 21.0 80 12.5 81 7.5 82 5.5 83 4.0 84 2.5 85 2.5 86 2.0 87 1.0 88 0.5 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.025453021358478656 2 0.006914737469053368 3 0.009884256627542545 4 4.24217022641311E-5 5 0.005090604271695731 6 0.0 7 0.0 8 4.24217022641311E-5 9 8.48434045282622E-5 10-11 0.0 12-13 2.7574106471685213E-4 14-15 0.0 16-17 0.0 18-19 0.004772441504714748 20-21 0.001315072770188064 22-23 0.0 24-25 0.005302712783016386 26-27 0.006193568530563139 28-29 0.008441918750562088 30-31 0.03190112010262658 32-33 8.908557475467529E-4 34-35 0.0046027546956582234 36-37 0.016502042180746995 38-39 0.00780559321660012 40-41 0.009375196200372972 42-43 0.006638996404336516 44-45 0.0025453021358478657 46-47 0.0020362417086782925 48-49 0.0014423378769804571 50-51 0.0032876819254701595 52-53 0.0029270974562250456 54-55 0.0016756572394331782 56-57 7.848014918864252E-4 58-59 0.0010393317054712117 60-61 0.0 62-63 4.24217022641311E-5 64-65 0.0066602072554685825 66-67 6.363255339619664E-4 68-69 8.908557475467529E-4 70-71 0.0037755315015076674 72-73 0.011135696844334412 74-75 0.00562087554999737 76-77 0.0035846338413190776 78-79 0.010584214714900708 80-81 1.0605425566032772E-4 82-83 0.005133025973959862 84-85 9.75699152075015E-4 86-87 0.0 88-89 0.0013999161747163262 90-91 6.999580873581631E-4 92-93 3.393736181130488E-4 94-95 7.848014918864252E-4 96-97 3.817953203771798E-4 98-99 1.696868090565244E-4 100-101 0.0010393317054712117 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2357284.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.66295052290114 #Duplication Level Percentage of deduplicated Percentage of total 1 73.90014793594719 40.39600130257757 2 14.51457641717893 15.86819145106239 3 4.95668936901203 8.128417972070839 4 2.2651739663944093 4.952843698031254 5 1.1942917689120567 3.2641755936973915 6 0.7210319683329103 2.364824088624714 7 0.4887863344659933 1.8702952252029363 8 0.3367892875591673 1.4727916929991913 9 0.24291956831700828 1.1950830309561418 >10 1.2604771972334152 12.129204134565217 >50 0.07880420004047976 2.9043994401989024 >100 0.0379848646890753 3.7723456146594128 >500 0.0017065560727538322 0.5975353264856301 >1k 4.654243834779228E-4 0.4850416264309071 >5k 1.5514146115930757E-4 0.5988498024375996 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8077 0.34264008918738686 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6046 0.2564816118889366 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3946 0.1673960371342613 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 8.484340452826218E-5 0.0 3 0.0 0.0 0.0 8.484340452826218E-5 0.0 4 0.0 0.0 0.0 8.484340452826218E-5 0.0 5 0.0 0.0 0.0 8.484340452826218E-5 0.0 6 0.0 0.0 0.0 8.484340452826218E-5 0.0 7 4.242170226413109E-5 0.0 0.0 8.484340452826218E-5 0.0 8 4.242170226413109E-5 0.0 0.0 8.484340452826218E-5 0.0 9 4.242170226413109E-5 0.0 0.0 1.6968680905652436E-4 0.0 10-11 4.242170226413109E-5 0.0 0.0 2.5453021358478657E-4 0.0 12-13 4.242170226413109E-5 0.0 0.0 2.9695191584891764E-4 0.0 14-15 6.363255339619664E-5 0.0 0.0 2.9695191584891764E-4 0.0 16-17 8.484340452826218E-5 0.0 0.0 2.9695191584891764E-4 0.0 18-19 1.0605425566032773E-4 0.0 0.0 3.1816276698098315E-4 0.0 20-21 1.2726510679239328E-4 0.0 0.0 3.8179532037717985E-4 0.0 22-23 1.2726510679239328E-4 0.0 0.0 5.939038316978354E-4 0.0 24-25 1.2726510679239328E-4 0.0 0.0 8.060123430184907E-4 0.0 26-27 1.2726510679239328E-4 0.0 0.0 0.0012090185145277362 0.0 28-29 1.6968680905652436E-4 0.0 0.0 0.0018877657507538337 0.0 30-31 1.6968680905652436E-4 0.0 0.0 0.0031604168186777663 0.0 32-33 1.6968680905652436E-4 0.0 0.0 0.004623965546790289 0.0 34-35 1.6968680905652436E-4 0.0 0.0 0.006511731297544123 0.0 36-37 1.6968680905652436E-4 0.0 0.0 0.008420707899430023 0.0 38-39 1.9089766018858992E-4 0.0 0.0 0.011156907695466477 0.0 40-41 2.1210851132065546E-4 0.0 0.0 0.015483921326407848 0.0 42-43 2.1210851132065546E-4 0.0 0.0 0.020213941128858465 0.0 44-45 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.03009819775640101 0.0 46-47 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.051436313995258956 0.0 48-49 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.06666570510808201 0.0 50-51 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.08365559686486651 0.0 52-53 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.10751780438844025 0.0 54-55 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.1391219725752179 0.0 56-57 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.1719351592765233 0.0 58-59 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.21132370982876902 0.0 60-61 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.2641599399987443 0.0 62-63 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.3248653959387159 0.0 64-65 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.3711050514066188 0.0 66-67 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.4517911291129961 0.0 68-69 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.559839204779738 0.0 70-71 2.1210851132065546E-4 4.242170226413109E-5 0.0 0.6252958913732923 0.0 72-73 2.33319362452721E-4 4.242170226413109E-5 0.0 0.7458159475056887 0.0 74-75 2.5453021358478657E-4 4.242170226413109E-5 0.0 0.8421768441986626 0.0 76-77 2.5453021358478657E-4 4.242170226413109E-5 0.0 0.9173693114618349 0.0 78-79 2.5453021358478657E-4 4.242170226413109E-5 0.0 1.0131150934719788 4.242170226413109E-5 80-81 2.5453021358478657E-4 4.242170226413109E-5 0.0 1.1178542763621184 4.242170226413109E-5 82-83 2.5453021358478657E-4 4.242170226413109E-5 0.0 1.2556823870182803 4.242170226413109E-5 84-85 2.5453021358478657E-4 4.242170226413109E-5 0.0 1.466539458122144 4.242170226413109E-5 86-87 2.5453021358478657E-4 4.242170226413109E-5 0.0 1.7562372628838951 4.242170226413109E-5 88-89 2.5453021358478657E-4 4.242170226413109E-5 0.0 2.091856560346568 4.242170226413109E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9055 0.0 29.542221 1 GTATCAA 12935 0.0 29.276224 1 GTACATG 10900 0.0 24.803272 1 TATCAAC 15585 0.0 24.200096 2 ATCAACG 15590 0.0 24.007996 3 TCAACGC 15625 0.0 23.98258 4 TACATGG 11130 0.0 23.814552 2 CAACGCA 15785 0.0 23.7094 5 ACATGGG 10930 0.0 23.249273 3 AACGCAG 16210 0.0 23.116096 6 GAGTACT 8005 0.0 21.388853 12-13 ACGCAGA 17960 0.0 20.625704 7 CGCAGAG 18015 0.0 20.430918 8 GTACTTT 8490 0.0 20.140087 14-15 TACACCG 285 1.9699655E-9 19.997482 5 AGTACTT 8175 0.0 19.694977 12-13 CATGGGA 6605 0.0 19.414663 4 GCAGAGT 18975 0.0 19.02183 9 CATGGGG 4890 0.0 18.939331 4 AGAGTAC 15490 0.0 18.2579 10-11 >>END_MODULE