FastQCFastQC Report
Thu 26 May 2016
SRR936841_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936841_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432229
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69840.28714401481110535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49010.20150240787360071No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32130.13210104805098533No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA131550.030.1922551
GGTATCA89200.027.8026221
ATCAACG155800.025.2825453
TATCAAC156950.025.278942
TCAACGC157000.025.0287724
CAACGCA158600.024.7463175
GTACATG121600.024.5752091
AACGCAG163300.024.1509676
TACATGG125600.023.2280332
ACATGGG123600.022.7581483
GAGTACT66850.022.42057812-13
CATGGGG54350.021.7687514
ACGCAGA180750.021.608637
CGCAGAG182250.021.3004238
GTACTTT71650.020.71646714-15
GCAGAGT188700.019.9403789
AGTACTT71500.019.23496412-13
AGAGTAC150150.018.57346310-11
GTATAGC10300.017.9890211
GTGTAGC12600.017.7217851
Produced by FastQC (version 0.11.5)