FastQCFastQC Report
Thu 26 May 2016
SRR936841_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936841_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432229
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76830.3158830850220107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62130.25544469702482786No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38300.15746872518993893No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA132100.030.0282631
GGTATCA90600.027.7379281
GTACATG112100.025.4692061
ATCAACG156900.025.0037673
TCAACGC158700.024.7800244
CAACGCA159300.024.6917695
TATCAAC160800.024.663672
TACATGG113600.024.4587782
AACGCAG165000.023.8675676
ACATGGG111100.023.8543993
CATGGGG48250.021.754214
GAGTACT81700.021.33454712-13
ACGCAGA183600.021.2684947
CGCAGAG184450.021.041718
AGTACTT84700.020.83122312-13
GTACTTT86600.019.8810114-15
GCAGAGT196300.019.4084879
AGAGTAC154450.019.0538210-11
CATGGGA69900.018.8213544
TACTTTT98650.017.6210714-15
Produced by FastQC (version 0.11.5)