##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936841_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2432229 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28853615346252 33.0 31.0 34.0 31.0 34.0 2 32.40722686885157 34.0 31.0 34.0 31.0 34.0 3 32.52253632367676 34.0 31.0 34.0 31.0 34.0 4 36.141527380851066 37.0 35.0 37.0 35.0 37.0 5 36.0556756785648 37.0 35.0 37.0 35.0 37.0 6 36.09547168461523 37.0 35.0 37.0 35.0 37.0 7 36.04837167881807 37.0 35.0 37.0 35.0 37.0 8 36.00988763804724 37.0 35.0 37.0 35.0 37.0 9 37.72257834274651 39.0 37.0 39.0 35.0 39.0 10-11 37.635402751961266 39.0 37.0 39.0 35.0 39.0 12-13 37.54540670306949 39.0 37.0 39.0 35.0 39.0 14-15 38.79441101146315 40.0 38.0 41.0 35.0 41.0 16-17 38.71096944407784 40.0 38.0 41.0 35.0 41.0 18-19 38.8535189326334 40.0 38.0 41.0 35.0 41.0 20-21 38.87302367499113 40.0 38.0 41.0 35.0 41.0 22-23 38.79828975807788 40.0 38.0 41.0 35.0 41.0 24-25 38.69927091569092 40.0 38.0 41.0 34.0 41.0 26-27 38.53709560242888 40.0 38.0 41.0 34.0 41.0 28-29 38.46994403076355 40.0 38.0 41.0 34.0 41.0 30-31 38.491180723525616 40.0 38.0 41.0 34.0 41.0 32-33 38.35846398509351 40.0 38.0 41.0 34.0 41.0 34-35 38.232649968403464 40.0 38.0 41.0 34.0 41.0 36-37 38.09192329340699 40.0 38.0 41.0 33.5 41.0 38-39 37.955585391013756 40.0 37.5 41.0 33.0 41.0 40-41 37.78902479988521 40.0 37.0 41.0 33.0 41.0 42-43 37.56289374890275 40.0 37.0 41.0 33.0 41.0 44-45 37.32261805940148 40.0 37.0 41.0 32.0 41.0 46-47 37.01286659274271 40.0 36.5 41.0 31.5 41.0 48-49 36.701492129236186 39.5 36.0 41.0 31.0 41.0 50-51 36.44017504108372 39.0 36.0 41.0 30.0 41.0 52-53 36.20458147649748 39.0 35.5 40.0 30.0 41.0 54-55 35.92802240249581 39.0 35.0 40.0 29.0 41.0 56-57 35.65467540268618 38.5 35.0 40.0 28.5 41.0 58-59 35.309828556439385 38.0 34.5 40.0 28.0 41.0 60-61 34.98846305179323 38.0 34.0 40.0 28.0 41.0 62-63 34.623201392632026 37.0 34.0 40.0 27.0 41.0 64-65 34.24111689318728 37.0 33.5 39.5 26.0 41.0 66-67 33.84773596565126 36.0 33.0 39.0 26.0 40.0 68-69 33.54388916504161 36.0 33.0 39.0 26.0 40.0 70-71 33.08657470164199 35.0 32.5 38.5 25.0 40.0 72-73 32.58600588184747 35.0 32.0 37.5 24.0 39.5 74-75 32.07918374462273 35.0 31.0 37.0 22.5 39.0 76-77 30.64435811759501 33.5 29.5 35.5 21.0 37.5 78-79 31.402262698125874 34.0 31.0 36.0 22.0 38.0 80-81 31.190012741398938 34.0 31.0 36.0 20.5 37.0 82-83 30.86915993518702 34.0 31.0 35.0 20.0 37.0 84-85 30.52363469887087 34.0 31.0 35.0 19.5 36.5 86-87 30.18287751687855 34.0 30.0 35.0 18.0 36.0 88-89 29.80571442902786 34.0 30.0 35.0 15.0 36.0 90-91 29.36913773333021 34.0 29.5 35.0 7.5 35.0 92-93 28.907589910325058 34.0 29.0 35.0 2.0 35.0 94-95 28.352176953732563 33.0 29.0 35.0 2.0 35.0 96-97 27.722456232534025 33.0 27.5 35.0 2.0 35.0 98-99 26.925217362345407 33.0 26.5 34.0 2.0 35.0 100-101 23.35390232580896 29.0 13.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1611325295217938 1101 2 0.08457484591659892 1101 3 0.16778416502742033 1101 4 0.15508182106631807 1101 5 0.1609214744470009 1101 6 0.1568718732450165 1101 7 0.1832382938187962 1101 8 0.17959628014724416 1101 9 0.25992762544335335 1101 10-11 0.21918229610886186 1101 12-13 0.2742768639665698 1101 14-15 0.31207946541873355 1101 16-17 0.32615417606924524 1101 18-19 0.21177875436806204 1101 20-21 0.2978080559909344 1101 22-23 0.22866095870195124 1101 24-25 0.26330818736752803 1101 26-27 0.2705620614744362 1101 28-29 0.26943288674936383 1101 30-31 0.2507606202848436 1101 32-33 0.2671579312928145 1101 34-35 0.307455895572204 1101 36-37 0.20790083584545727 1101 38-39 0.19781039344597673 1101 40-41 0.22942948707102317 1101 42-43 0.26482618795896684 1101 44-45 0.17779254831014413 1101 46-47 0.21047910198838338 1101 48-49 0.14691075535369436 1101 50-51 -0.024542301662741295 1101 52-53 -0.05615433149513649 1101 54-55 -0.17150120968442906 1101 56-57 -0.038553222958022104 1101 58-59 0.05280778982668011 1101 60-61 0.10076660633648515 1101 62-63 0.031112319409864142 1101 64-65 -0.0036733713895387154 1101 66-67 -0.15935354347502795 1101 68-69 -0.21302847921871404 1101 70-71 -0.21886463957675062 1101 72-73 -0.08099302154555943 1101 74-75 -0.09763001783848324 1101 76-77 -0.16713639146118808 1101 78-79 -0.16793606364202418 1101 80-81 0.0653136043015401 1101 82-83 0.05679210517515898 1101 84-85 -0.0823520094667316 1101 86-87 -0.33424609961291907 1101 88-89 -0.16134941569200478 1101 90-91 0.054346942721018365 1101 92-93 0.1306231202074386 1101 94-95 -8.832811332233348E-4 1101 96-97 -0.054025479958557554 1101 98-99 0.0983389353701618 1101 100-101 0.5845134407530814 1102 1 0.27192896991770965 1102 2 0.2573036530530075 1102 3 0.17941208337066428 1102 4 0.15402170134626658 1102 5 0.18433892766373816 1102 6 0.13903067908132982 1102 7 0.12519352147815965 1102 8 0.16966680165054981 1102 9 0.16448117118294903 1102 10-11 0.23320953566268798 1102 12-13 0.2161278228967447 1102 14-15 0.27769929576699326 1102 16-17 0.36189358635790114 1102 18-19 0.33946078231664245 1102 20-21 0.3110981645053599 1102 22-23 0.24389054703922852 1102 24-25 0.3210630437650508 1102 26-27 0.3367270054805047 1102 28-29 0.3288051303484849 1102 30-31 0.2799328927533651 1102 32-33 0.3295962457497126 1102 34-35 0.2575407442505764 1102 36-37 0.2585760899108678 1102 38-39 0.2770217178119836 1102 40-41 0.2239397384792241 1102 42-43 0.29325538281212715 1102 44-45 0.26324346005872457 1102 46-47 0.21833795015128032 1102 48-49 0.15049849569718532 1102 50-51 0.24110077735373636 1102 52-53 0.1751872681761526 1102 54-55 0.25325741635349885 1102 56-57 0.2408538170710699 1102 58-59 0.31883560416186896 1102 60-61 0.28879945619961944 1102 62-63 0.31115873854474074 1102 64-65 0.2845678367200932 1102 66-67 0.23783659348520558 1102 68-69 0.1262534122681629 1102 70-71 0.10437436748517115 1102 72-73 0.31222431391267946 1102 74-75 0.40586502108796907 1102 76-77 0.2799635204643174 1102 78-79 0.22056001634251388 1102 80-81 0.3282026855083089 1102 82-83 0.29530739341465306 1102 84-85 0.23047576498566968 1102 86-87 0.18786115185360686 1102 88-89 0.23457426350831057 1102 90-91 0.40512511328054757 1102 92-93 0.3678254370586025 1102 94-95 0.25129503924743446 1102 96-97 0.40817026307506765 1102 98-99 0.5655223078965896 1102 100-101 0.7234720503035099 1103 1 0.20809980747161205 1103 2 0.1991394160007829 1103 3 0.1936888573344291 1103 4 0.1993231065736225 1103 5 0.11057522713907986 1103 6 0.12316124675478335 1103 7 0.03075210124622174 1103 8 0.07754332452748969 1103 9 0.11490194369172002 1103 10-11 0.2131274840762174 1103 12-13 0.20852243716019103 1103 14-15 0.3317505336452342 1103 16-17 0.21796940119021002 1103 18-19 0.25578069951355786 1103 20-21 0.261448681961447 1103 22-23 0.2409035232264003 1103 24-25 0.1630145933546956 1103 26-27 0.203598890576707 1103 28-29 0.2599236034172776 1103 30-31 0.1857589153511796 1103 32-33 0.24520477133454222 1103 34-35 0.2704582013523691 1103 36-37 0.08861759249121093 1103 38-39 0.10895189723549237 1103 40-41 0.2701418292695976 1103 42-43 0.3082056580224872 1103 44-45 0.28585785107213013 1103 46-47 0.28179341886773557 1103 48-49 0.25773786050310576 1103 50-51 0.28276628625923195 1103 52-53 0.3723330500943618 1103 54-55 0.33545724887807893 1103 56-57 0.2565562160364081 1103 58-59 0.20964346464449335 1103 60-61 0.2386804488766714 1103 62-63 0.28287310707654 1103 64-65 0.15804117074387847 1103 66-67 0.14797611844106484 1103 68-69 0.026596856856038187 1103 70-71 0.10881117140758789 1103 72-73 0.21375483207606294 1103 74-75 0.3839661264762313 1103 76-77 0.2618498733650796 1103 78-79 0.33234843527860747 1103 80-81 0.38211372903423424 1103 82-83 0.33200547540435466 1103 84-85 0.354501193209952 1103 86-87 0.04448716636814254 1103 88-89 0.16817092435669778 1103 90-91 0.06396389356442356 1103 92-93 0.1479365947608251 1103 94-95 0.08781058440138523 1103 96-97 0.07835073488589828 1103 98-99 0.20234804884268698 1103 100-101 0.5472322775446621 1104 1 0.1941942602452258 1104 2 0.2414319061193595 1104 3 0.2500436344913055 1104 4 0.0789298940889509 1104 5 0.132109914340937 1104 6 0.0033336605478879733 1104 7 0.14486209170554076 1104 8 0.16203971269703743 1104 9 0.1376530681295023 1104 10-11 0.22089462323391018 1104 12-13 0.17747943593353455 1104 14-15 0.12956299105289304 1104 16-17 0.2627680246057764 1104 18-19 0.18815939652868963 1104 20-21 0.1701051491823833 1104 22-23 0.21076859069266618 1104 24-25 0.22425726747218278 1104 26-27 0.1790214065914313 1104 28-29 0.12079998340909981 1104 30-31 0.15599198063672048 1104 32-33 0.21715345608308212 1104 34-35 0.26833880650434594 1104 36-37 0.22736639990568364 1104 38-39 0.20537925174006233 1104 40-41 0.2907752625529483 1104 42-43 0.37446570981477834 1104 44-45 0.3426402553245538 1104 46-47 0.40766059891407025 1104 48-49 0.33145497921646694 1104 50-51 0.4423478591091694 1104 52-53 0.33305609766149047 1104 54-55 0.2284022536484187 1104 56-57 0.28006037304881204 1104 58-59 0.30976953797146933 1104 60-61 0.41654435783128463 1104 62-63 0.33762470854852467 1104 64-65 0.39822108078885066 1104 66-67 0.3508826197358701 1104 68-69 0.28800649294708336 1104 70-71 0.14955500403672062 1104 72-73 0.3179322888022469 1104 74-75 0.426127432187009 1104 76-77 0.42645223127703247 1104 78-79 0.41891481118156193 1104 80-81 0.6600202303290423 1104 82-83 0.5675172649641205 1104 84-85 0.5179552616302878 1104 86-87 0.3257056480364007 1104 88-89 0.15985860324452617 1104 90-91 0.3395244087613669 1104 92-93 0.22067408964974788 1104 94-95 0.31706470733991665 1104 96-97 0.34224946733785444 1104 98-99 0.26881765494872667 1104 100-101 0.6681774867582355 1105 1 0.2216290009538895 1105 2 0.1896987192809334 1105 3 0.14207533574101205 1105 4 0.12067489052745373 1105 5 0.16938619535665111 1105 6 0.18170036647642718 1105 7 0.09372264376166584 1105 8 0.15426244703069614 1105 9 0.2366994545625829 1105 10-11 0.13175285992560504 1105 12-13 0.21588057747648293 1105 14-15 0.23322691559560838 1105 16-17 0.23529285813076228 1105 18-19 0.1456705970265304 1105 20-21 0.221267797326135 1105 22-23 0.23174467508863472 1105 24-25 0.23262803925515385 1105 26-27 0.3410974597951508 1105 28-29 0.2623343934179303 1105 30-31 0.26520083054306554 1105 32-33 0.26697995893616167 1105 34-35 0.23866838900937637 1105 36-37 0.25741104671173076 1105 38-39 0.379007685408439 1105 40-41 0.3460245599515801 1105 42-43 0.3646836830639373 1105 44-45 0.39932105937054274 1105 46-47 0.38974965664455397 1105 48-49 0.24253319104749238 1105 50-51 0.19700954743193932 1105 52-53 0.23309877361445075 1105 54-55 0.35441456604880983 1105 56-57 0.25048539450317975 1105 58-59 0.20967192642816457 1105 60-61 0.2825105957210141 1105 62-63 0.28348180462839423 1105 64-65 0.05771415254186962 1105 66-67 0.1147867555788551 1105 68-69 0.10013849044549517 1105 70-71 -0.10715753539973605 1105 72-73 0.030988026700512705 1105 74-75 0.20917654367367078 1105 76-77 -0.11367713117526534 1105 78-79 0.23547090833375606 1105 80-81 0.21818677827896593 1105 82-83 0.16871576090835205 1105 84-85 0.17681789316921126 1105 86-87 0.05023213894021694 1105 88-89 -0.028382076715310234 1105 90-91 2.40806103974478E-4 1105 92-93 -0.06437861903178188 1105 94-95 -0.02166643570832605 1105 96-97 0.018633758862723226 1105 98-99 -0.06188722764584753 1105 100-101 0.22717582782755485 1106 1 0.13897617550768615 1106 2 0.15368487054623614 1106 3 0.039045784134188466 1106 4 0.12388863481428558 1106 5 0.11255509844744438 1106 6 0.09272759994182422 1106 7 0.0974971378188414 1106 8 0.1152433740242742 1106 9 0.1536263016084547 1106 10-11 0.10280121778352935 1106 12-13 0.1562586468065703 1106 14-15 0.20632431134968954 1106 16-17 0.1030096860716938 1106 18-19 -0.0058476071465065615 1106 20-21 0.14394538217593578 1106 22-23 0.10833464020379324 1106 24-25 0.16376735692506372 1106 26-27 0.1183269332539254 1106 28-29 0.1303520255698345 1106 30-31 0.1251047328730408 1106 32-33 0.11085881632162398 1106 34-35 0.14297648766375914 1106 36-37 0.05036757874210451 1106 38-39 0.015818888381069485 1106 40-41 0.12397164283171236 1106 42-43 -0.03279280207760138 1106 44-45 -0.20763538436756335 1106 46-47 -0.276664343877691 1106 48-49 -0.17945965915811968 1106 50-51 -0.2935962501759022 1106 52-53 -0.16085729253336467 1106 54-55 -0.32750059636197904 1106 56-57 -0.2813500700549909 1106 58-59 -0.20038925822851894 1106 60-61 -0.347447390313782 1106 62-63 -0.22667908453100694 1106 64-65 -0.3966596487028582 1106 66-67 -0.24767503923369105 1106 68-69 -0.2659738135503886 1106 70-71 -0.28645844856711733 1106 72-73 -0.20943578553379183 1106 74-75 -0.16534160602115833 1106 76-77 -0.24876099942818897 1106 78-79 -0.2551611890958121 1106 80-81 -0.1734257858972974 1106 82-83 -0.08797472588256028 1106 84-85 -0.0886496894826756 1106 86-87 -0.21948192564087776 1106 88-89 -0.22479572031139483 1106 90-91 -0.2040439984226019 1106 92-93 -0.11938727918736802 1106 94-95 -0.20863045943076486 1106 96-97 0.05495663050268362 1106 98-99 0.11496216642504464 1106 100-101 0.10523975705849509 1107 1 0.04835067749471733 1107 2 0.08226651706651467 1107 3 0.0717693927408547 1107 4 0.07908942736859359 1107 5 0.09506896124620567 1107 6 0.08586709878549215 1107 7 0.05977332216426845 1107 8 0.09949738040134548 1107 9 0.07730812794670783 1107 10-11 0.11575441742665049 1107 12-13 0.08479402125585267 1107 14-15 0.1478877050280687 1107 16-17 0.13309359066904136 1107 18-19 0.061484530328293374 1107 20-21 -0.06258020329908476 1107 22-23 0.07227442314229648 1107 24-25 0.06510385118983919 1107 26-27 0.10364519726385879 1107 28-29 0.18705993869002668 1107 30-31 0.15199696255317008 1107 32-33 0.12420666183125206 1107 34-35 0.15384242476888232 1107 36-37 0.17431147754209775 1107 38-39 0.2504405616544432 1107 40-41 0.27252051931445465 1107 42-43 0.2412107955366949 1107 44-45 0.1347884502350425 1107 46-47 0.08034930999090761 1107 48-49 0.16826005318331738 1107 50-51 0.1543851581765452 1107 52-53 0.36731361485097125 1107 54-55 0.39685322593067696 1107 56-57 0.3189382912172576 1107 58-59 0.37846525234159145 1107 60-61 0.36486879192352006 1107 62-63 0.30120540657659944 1107 64-65 0.3889358085404879 1107 66-67 0.37198610490052175 1107 68-69 0.4282967315628454 1107 70-71 0.41245216571265786 1107 72-73 0.2991151363313449 1107 74-75 0.1381446729765301 1107 76-77 0.3014114150139662 1107 78-79 0.3600848151932219 1107 80-81 0.46923257752072445 1107 82-83 0.34841074462440247 1107 84-85 0.4457212048513668 1107 86-87 0.29557484940595913 1107 88-89 0.2487212833414283 1107 90-91 0.4610506726661079 1107 92-93 0.4374591364368463 1107 94-95 0.5664982553538387 1107 96-97 0.47953158623834824 1107 98-99 0.3392424239675478 1107 100-101 0.45483748806436 1108 1 0.09803211762854858 1108 2 0.08969720206329868 1108 3 0.11179972232063307 1108 4 0.0843331932996847 1108 5 0.12971281206041851 1108 6 0.08590463846586971 1108 7 0.038731245456737895 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-0.9039475347335255 2316 62-63 -1.0203076620592952 2316 64-65 -0.7731710327463688 2316 66-67 -0.9384726757731343 2316 68-69 -1.2681884589685168 2316 70-71 -1.053107533699425 2316 72-73 -0.9194801789080671 2316 74-75 -0.9845196064983739 2316 76-77 -0.8338672421287114 2316 78-79 -0.9434361894097663 2316 80-81 -0.9534581861534832 2316 82-83 -0.8972690784425588 2316 84-85 -1.100161199108566 2316 86-87 -1.0225530429473864 2316 88-89 -1.3142263925539233 2316 90-91 -1.2418339367616475 2316 92-93 -1.2258588536137687 2316 94-95 -1.2564063953335172 2316 96-97 -1.0656012064662583 2316 98-99 -1.4275013204955513 2316 100-101 -1.6032169609713698 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 11.0 5 43.0 6 87.0 7 176.0 8 503.0 9 957.0 10 1716.0 11 2806.0 12 4396.0 13 6279.0 14 7885.0 15 10952.0 16 14654.0 17 20290.0 18 18038.0 19 11146.0 20 9804.0 21 10587.0 22 11105.0 23 12540.0 24 14281.0 25 17686.0 26 21886.0 27 27206.0 28 34638.0 29 44112.0 30 55269.0 31 70223.0 32 91008.0 33 120425.0 34 166707.0 35 240305.0 36 379479.0 37 575400.0 38 404091.0 39 25532.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24700122841988 19.19748443295488 14.787510502434836 22.768003836190392 2 15.511911032275757 20.648023311800262 39.9714153316944 23.868650324229577 3 19.53565954451564 24.829250924256975 29.809096530582707 25.825993000644676 4 13.098373583288996 17.126713148245756 37.15230295968536 32.62261030877988 5 13.958600920637373 37.537763729387216 33.859037886250796 14.644597463724619 6 31.60549438395809 36.232854718860764 17.7538792605466 14.407771636634545 7 28.58114922566913 32.76632257900058 20.649289191108238 18.003239004222053 8 26.453512395420002 34.61002232931192 19.847473243678944 19.08899203158913 9 26.531599833568237 16.812486329406617 20.26109394349543 36.394819893529714 10-11 24.7941809168399 26.86718508904144 27.369666760487917 20.968967233630746 12-13 26.025674391679406 24.125791609260478 27.913202251926112 21.935331747134008 14-15 22.9246711555532 25.241208784205764 26.401975307423765 25.43214475281727 16-17 22.17525570166296 28.678590708358463 26.872490213709316 22.273663376269255 18-19 22.11270168066795 27.27432887889344 29.223476107708297 21.389493332730318 20-21 23.073391939876863 26.24485113015599 29.174328598884514 21.507428331082636 22-23 22.64648985574581 26.283743084864717 29.009442204784623 22.060324854604847 24-25 22.460005851272815 26.450281965147717 29.132286669603953 21.95742551397552 26-27 22.44280574955352 26.566737298806846 29.05883821059671 21.93161874104292 28-29 22.11367954336424 26.655820621711086 29.243700104801245 21.98679973012343 30-31 22.37252992094678 26.450861148469322 29.1243705695228 22.0522383610611 32-33 22.240546487990684 26.54322662772109 29.0588385732236 22.157388311064622 34-35 22.570140393852718 26.545876231226583 28.863421166345766 22.02056220857493 36-37 22.4800586839451 26.406171682611635 28.89183067017378 22.22193896326949 38-39 22.42731271645817 26.55668441336687 29.044052867964755 21.9719500022102 40-41 22.596694668841934 26.442567034181515 28.958897981738286 22.001840315238272 42-43 22.375915261268574 26.566824094277308 28.683545011592248 22.373715632861874 44-45 22.590204294085794 26.67947796034008 28.400676909945567 22.329640835628553 46-47 22.5731311287799 26.89584242566524 28.058770092468887 22.472256353085974 48-49 22.748684706079707 26.96167515570547 27.846384031287467 22.44325610692735 50-51 22.716324310277763 27.05502235547884 27.828973391347112 22.399679942896285 52-53 22.88904539827459 27.111119059924043 27.629532416561105 22.370303125240262 54-55 22.65299204056338 26.911702820288845 27.790844664040314 22.64446047510746 56-57 22.646079900663818 27.295967140117057 27.559273809744074 22.498679149475052 58-59 22.84019776130909 27.04363288758236 27.616071703892526 22.500097647216027 60-61 22.815704727926082 26.867164364736006 28.01528247603248 22.301848431305434 62-63 22.675449984694364 26.96462599047206 27.907202407127475 22.452721617706104 64-65 22.639366046275267 27.00090205359037 27.763700607015462 22.5960312931189 66-67 22.53775115873557 27.286007556193486 27.535968804901945 22.640272480169003 68-69 22.518812016337325 27.114815177232998 27.745349725477364 22.621023080952316 70-71 22.591381454344887 27.066861249765388 27.619239880133634 22.72251741575609 72-73 22.68345209271002 26.93734841579473 27.603404120253476 22.77579537124177 74-75 22.574827452513723 27.148841659235213 27.481067777746254 22.79526311050481 76-77 22.642090855754123 27.006564760143885 27.5196948971499 22.83164948695209 78-79 22.618930146732318 27.0248673620088 27.50834229647099 22.847860194787895 80-81 22.62668968576789 27.229900052750967 27.406922094266754 22.736488167214393 82-83 22.58952352462867 27.264103520907412 27.363508427829675 22.782864526634246 84-85 22.643462128498022 27.05683223186337 27.31850416088794 22.98120147875067 86-87 22.59320415733673 26.93077355112835 27.47397964092439 23.002042650610534 88-89 22.640844230758162 26.841769638183294 27.401359906521844 23.1160262245367 90-91 22.507600071375297 26.852735820121094 27.599339531801064 23.04032457670254 92-93 22.56652031245439 26.77338504999739 27.593456531812205 23.06663810573602 94-95 22.357093989543745 26.799934052235237 27.555352540962442 23.28761941725858 96-97 22.270151302441864 26.67574356601265 27.649087866181038 23.405017265364446 98-99 22.195485871557523 26.738621141639662 27.420764694805417 23.645128291997406 100-101 22.083183950162486 26.841647808754455 27.391884957964397 23.683283283118662 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 339.0 1 258.0 2 205.5 3 228.5 4 211.0 5 220.0 6 377.5 7 769.0 8 1243.0 9 1586.5 10 1571.5 11 1450.5 12 1592.5 13 1804.5 14 2041.5 15 2271.5 16 2457.0 17 2716.0 18 2890.5 19 3079.5 20 3449.0 21 3979.0 22 4721.0 23 5617.5 24 6813.5 25 8587.0 26 11041.0 27 14158.5 28 17346.0 29 21022.5 30 25449.5 31 29972.5 32 34413.0 33 39188.0 34 45094.5 35 50118.0 36 55452.5 37 62122.5 38 67490.0 39 70901.5 40 74097.5 41 78145.5 42 82030.0 43 85855.0 44 89320.0 45 91643.0 46 93448.5 47 97140.0 48 106821.5 49 107388.5 50 98946.0 51 94436.0 52 89555.5 53 85606.0 54 81576.0 55 75189.0 56 67984.0 57 60809.0 58 54748.0 59 48592.5 60 40702.5 61 32479.5 62 24866.5 63 18661.5 64 13587.0 65 10009.5 66 7364.0 67 5499.0 68 3961.0 69 2513.5 70 1678.5 71 1161.5 72 785.5 73 549.5 74 381.0 75 276.0 76 174.0 77 83.5 78 41.5 79 15.0 80 8.0 81 5.0 82 6.0 83 4.0 84 1.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.026724457277665876 2 0.00641386974663981 3 2.0557274828973752E-4 4 4.522600462374226E-4 5 4.11145496579475E-5 6 0.0 7 0.0 8 0.0 9 4.11145496579475E-5 10-11 0.012313807622555276 12-13 0.0 14-15 0.0 16-17 0.0 18-19 4.728173210663963E-4 20-21 8.428482679879238E-4 22-23 0.00263133117810864 24-25 0.011491516629396329 26-27 0.005941052425573415 28-29 2.46687297947685E-4 30-31 0.0028780184760563254 32-33 0.004810402309979858 34-35 0.0 36-37 0.008962971825432556 38-39 0.013218327715030124 40-41 0.0015212383373440575 42-43 0.0 44-45 0.0 46-47 2.261300231187113E-4 48-49 0.003001362125030168 50-51 0.006701671594245443 52-53 0.0 54-55 0.003474179446096564 56-57 0.0034536221712675903 58-59 1.6445819863179E-4 60-61 0.0012951083142253464 62-63 0.004872074134466779 64-65 4.11145496579475E-5 66-67 0.00213795658221327 68-69 2.8780184760563256E-4 70-71 8.428482679879238E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0018501547346076376 80-81 0.0018912692842655851 82-83 0.010545881987263535 84-85 0.0072361607397987615 86-87 0.0039058822175050125 88-89 1.233436489738425E-4 90-91 6.167182448692126E-4 92-93 8.2229099315895E-5 94-95 4.11145496579475E-5 96-97 0.0019529411087525063 98-99 0.0017679256352917429 100-101 2.0557274828973752E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2432229.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.303795230018146 #Duplication Level Percentage of deduplicated Percentage of total 1 73.42629132360318 39.87326288536606 2 14.796319664781477 16.06992626468368 3 4.988820068413482 8.127355903035925 4 2.3261721246100335 5.052798988983982 5 1.2469879998785702 3.3858090499847893 6 0.7513101252405371 2.447939471718085 7 0.46747033558584905 1.7769789365833275 8 0.3465921264206952 1.50570142891888 9 0.2614728270841691 1.2779070174173397 >10 1.270530236044215 12.170323645107338 >50 0.07738460490547101 2.8754744979617715 >100 0.03814508121206506 3.741201848150851 >500 0.001896577439579423 0.6514409054687934 >1k 4.5517858549717823E-4 0.471412020568795 >5k 1.5172619516572606E-4 0.5724671360503342 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7683 0.3158830850220107 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6213 0.25544469702482786 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3830 0.15746872518993893 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.222909931589501E-5 0.0 10-11 0.0 0.0 0.0 1.0278637414486876E-4 0.0 12-13 0.0 0.0 0.0 1.233436489738425E-4 0.0 14-15 0.0 0.0 0.0 1.6445819863179003E-4 0.0 16-17 0.0 0.0 0.0 2.46687297947685E-4 0.0 18-19 0.0 0.0 0.0 3.2891639726358006E-4 0.0 20-21 0.0 0.0 0.0 3.7003094692152755E-4 0.0 22-23 0.0 0.0 0.0 4.1114549657947504E-4 0.0 24-25 0.0 0.0 0.0 5.344891455533175E-4 0.0 26-27 0.0 0.0 0.0 8.428482679879238E-4 0.0 28-29 4.111454965794751E-5 0.0 0.0 0.0014595665128571363 0.0 30-31 4.111454965794751E-5 0.0 0.0 0.0026518884529376143 0.0 32-33 4.111454965794751E-5 0.0 0.0 0.004255355889597566 0.0 34-35 4.111454965794751E-5 0.0 0.0 0.005632693303138809 0.0 36-37 4.111454965794751E-5 0.0 0.0 0.007750092610523104 0.0 38-39 4.111454965794751E-5 0.0 0.0 0.010011392841710216 0.0 40-41 4.111454965794751E-5 0.0 0.0 0.013382785913661913 0.0 42-43 6.167182448692127E-5 0.0 0.0 0.01767925635291743 0.0 44-45 8.222909931589501E-5 0.0 0.0 0.028245695615009933 0.0 46-47 1.0278637414486876E-4 0.0 0.0 0.047651763053561155 0.0 48-49 1.233436489738425E-4 0.0 0.0 0.06282303187734378 0.0 50-51 1.233436489738425E-4 0.0 0.0 0.08111900647513043 0.0 52-53 1.233436489738425E-4 0.0 0.0 0.10591107991887277 0.0 54-55 1.4390092380281628E-4 0.0 0.0 0.13508185290118652 0.0 56-57 1.6445819863179003E-4 0.0 0.0 0.1661233378929369 0.0 58-59 1.6445819863179003E-4 0.0 0.0 0.20421596815102527 0.0 60-61 1.6445819863179003E-4 0.0 0.0 0.2547663069554717 0.0 62-63 1.6445819863179003E-4 0.0 0.0 0.3144646330588115 0.0 64-65 1.6445819863179003E-4 0.0 0.0 0.35940283583494814 0.0 66-67 1.6445819863179003E-4 0.0 0.0 0.43203168780571233 0.0 68-69 1.6445819863179003E-4 0.0 0.0 0.537305492204887 0.0 70-71 1.6445819863179003E-4 0.0 0.0 0.60154697604543 0.0 72-73 1.6445819863179003E-4 0.0 0.0 0.7199157645106609 0.0 74-75 1.6445819863179003E-4 0.0 0.0 0.8136363804559521 0.0 76-77 1.6445819863179003E-4 0.0 0.0 0.8895543963993522 0.0 78-79 1.6445819863179003E-4 0.0 0.0 0.9865847335921083 0.0 80-81 1.6445819863179003E-4 0.0 0.0 1.0901728414553071 0.0 82-83 1.6445819863179003E-4 0.0 0.0 1.2235484405456887 0.0 84-85 1.6445819863179003E-4 0.0 0.0 1.4303546253251647 0.0 86-87 1.6445819863179003E-4 0.0 0.0 1.7141683616139765 0.0 88-89 1.6445819863179003E-4 0.0 0.0 2.041029031394659 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 13210 0.0 30.028263 1 GGTATCA 9060 0.0 27.737928 1 GTACATG 11210 0.0 25.469206 1 ATCAACG 15690 0.0 25.003767 3 TCAACGC 15870 0.0 24.780024 4 CAACGCA 15930 0.0 24.691769 5 TATCAAC 16080 0.0 24.66367 2 TACATGG 11360 0.0 24.458778 2 AACGCAG 16500 0.0 23.867567 6 ACATGGG 11110 0.0 23.854399 3 CATGGGG 4825 0.0 21.75421 4 GAGTACT 8170 0.0 21.334547 12-13 ACGCAGA 18360 0.0 21.268494 7 CGCAGAG 18445 0.0 21.04171 8 AGTACTT 8470 0.0 20.831223 12-13 GTACTTT 8660 0.0 19.88101 14-15 GCAGAGT 19630 0.0 19.408487 9 AGAGTAC 15445 0.0 19.05382 10-11 CATGGGA 6990 0.0 18.821354 4 TACTTTT 9865 0.0 17.62107 14-15 >>END_MODULE