FastQCFastQC Report
Thu 26 May 2016
SRR936840_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936840_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469706
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70570.28574251348136176No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51780.20966058308154897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32720.13248540514538976No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA128400.030.6530231
GGTATCA86250.027.5561771
GTACATG114400.026.177161
ATCAACG150050.025.8194643
TATCAAC151100.025.7344252
TCAACGC151000.025.6265814
CAACGCA153600.025.1927995
AACGCAG156600.024.7061736
TACATGG117350.024.5074372
ACATGGG118700.023.5078543
GAGTACT69000.022.2099612-13
ACGCAGA175900.021.7482177
CGCAGAG177850.021.269718
GTACTTT74900.020.0169514-15
GCAGAGT185250.019.9823449
CATGGGA71950.019.5570854
CATGGGG54650.019.3110564
AGTACTT74550.019.154212-13
CGTAGGG1507.684876E-419.014532
AGAGTAC147500.018.31420310-11
Produced by FastQC (version 0.11.5)