##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936840_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2469706 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.772102833292706 33.0 31.0 34.0 30.0 34.0 2 31.85018986065548 33.0 31.0 34.0 30.0 34.0 3 31.885238566857755 33.0 31.0 34.0 30.0 34.0 4 35.47980407384523 37.0 35.0 37.0 33.0 37.0 5 35.485501513135574 37.0 35.0 37.0 33.0 37.0 6 35.5477461689772 37.0 35.0 37.0 33.0 37.0 7 35.49015996235989 37.0 35.0 37.0 33.0 37.0 8 35.45341105378535 37.0 35.0 37.0 33.0 37.0 9 37.08151010687102 39.0 37.0 39.0 33.0 39.0 10-11 36.94747229022402 39.0 37.0 39.0 33.0 39.0 12-13 36.86511957293702 39.0 37.0 39.0 33.0 39.0 14-15 38.02239841503402 40.0 38.0 41.0 33.0 41.0 16-17 37.95517604119681 40.0 38.0 41.0 33.0 41.0 18-19 37.90284531843062 40.0 38.0 41.0 33.0 41.0 20-21 37.829765162331064 40.0 37.5 41.0 32.5 41.0 22-23 37.719313958827485 40.0 37.0 41.0 32.0 41.0 24-25 37.68317038546289 40.0 37.0 41.0 32.0 41.0 26-27 37.71697987533739 40.0 37.0 41.0 32.0 41.0 28-29 37.65233736323271 40.0 37.0 41.0 32.0 41.0 30-31 37.51480621580059 40.0 37.0 41.0 32.0 41.0 32-33 37.35682769528033 40.0 37.0 41.0 31.5 41.0 34-35 37.20946197644578 40.0 36.5 41.0 31.0 41.0 36-37 37.04791481253234 39.5 36.0 41.0 31.0 41.0 38-39 36.89914224608111 39.0 36.0 41.0 30.5 41.0 40-41 36.73195878375807 39.0 36.0 41.0 30.0 41.0 42-43 36.5290815991863 39.0 35.5 41.0 30.0 41.0 44-45 36.30735561236844 39.0 35.0 41.0 30.0 41.0 46-47 36.003695784032594 39.0 35.0 40.0 29.0 41.0 48-49 35.81405074126232 39.0 35.0 40.0 28.0 41.0 50-51 35.146819904879365 38.0 34.0 40.0 27.0 40.5 52-53 35.333564804879614 38.5 35.0 40.0 28.0 40.5 54-55 35.72592587943666 39.0 35.0 40.5 28.0 41.0 56-57 35.6144567410048 39.0 35.0 41.0 28.0 41.0 58-59 35.40522394163516 38.5 35.0 40.5 27.5 41.0 60-61 35.11801850098757 38.0 34.5 40.0 27.0 41.0 62-63 34.8054163127109 38.0 34.0 40.0 26.0 41.0 64-65 34.468783936225606 37.0 34.0 40.0 26.0 41.0 66-67 34.102965292225065 37.0 34.0 39.5 26.0 41.0 68-69 33.71813021468952 36.0 33.0 39.0 25.0 41.0 70-71 33.3080433460501 36.0 33.0 39.0 24.5 40.5 72-73 32.92148033004739 35.0 33.0 38.5 24.0 40.0 74-75 32.52236703478066 35.0 32.0 37.5 22.5 39.5 76-77 32.069320599294 35.0 32.0 37.0 21.5 39.0 78-79 31.615911367587884 35.0 31.5 36.5 20.5 39.0 80-81 31.18794362567852 35.0 31.0 36.0 19.5 37.5 82-83 30.801872368613914 34.5 31.0 35.5 18.0 37.0 84-85 30.439154093645154 34.0 31.0 35.0 16.0 37.0 86-87 30.091628315273155 34.0 30.0 35.0 11.5 36.0 88-89 29.741771692663015 34.0 30.0 35.0 7.0 36.0 90-91 29.390910294585673 34.0 30.0 35.0 3.5 35.5 92-93 29.020705096072163 34.0 29.0 35.0 2.0 35.0 94-95 28.662868576259683 34.0 29.0 35.0 2.0 35.0 96-97 28.17587559004999 34.0 29.0 35.0 2.0 35.0 98-99 27.621246820471747 34.0 28.5 35.0 2.0 35.0 100-101 25.4293057554219 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3316151207154867 1101 2 0.40017777188528925 1101 3 0.4075752217534685 1101 4 0.46189248025527263 1101 5 0.3848015226031194 1101 6 0.2803281612916706 1101 7 0.34215583042014686 1101 8 0.2963387231995114 1101 9 0.3024650534562596 1101 10-11 0.41414276642768044 1101 12-13 0.5765435678100914 1101 14-15 0.6220503652349834 1101 16-17 0.6745462826442292 1101 18-19 0.5346958777186614 1101 20-21 0.47479687217971644 1101 22-23 0.6615568862446537 1101 24-25 0.5863811259406262 1101 26-27 0.5893854868546597 1101 28-29 0.6268017431260517 1101 30-31 0.579877812233768 1101 32-33 0.5271857666907778 1101 34-35 0.551256851568013 1101 36-37 0.46722189175936535 1101 38-39 0.45982095117363286 1101 40-41 0.5703204677642049 1101 42-43 0.5376673413311224 1101 44-45 0.49757005184245173 1101 46-47 0.568422971285024 1101 48-49 0.4726214524817465 1101 50-51 0.5471173019833699 1101 52-53 0.5006616253737448 1101 54-55 0.6886527913829994 1101 56-57 0.5116277725340908 1101 58-59 0.5634146898893491 1101 60-61 0.663317207223102 1101 62-63 0.6725205791681361 1101 64-65 0.4990064528749656 1101 66-67 0.5288971697515024 1101 68-69 0.6272942618807704 1101 70-71 0.5218972467583143 1101 72-73 0.4832015366074245 1101 74-75 0.4578137338554882 1101 76-77 0.6634946201145553 1101 78-79 0.7097665323382785 1101 80-81 0.6544169980015688 1101 82-83 0.6131316739556567 1101 84-85 0.45839579203002145 1101 86-87 0.4899659012753723 1101 88-89 0.4471813243100371 1101 90-91 0.5416874623655055 1101 92-93 0.6002053577408262 1101 94-95 0.5990473176127331 1101 96-97 0.7107673348974437 1101 98-99 0.7306301093776213 1101 100-101 0.8017053590028347 1102 1 0.22359098154731782 1102 2 0.2109996441907569 1102 3 0.2922267171045121 1102 4 0.17259136414489973 1102 5 0.25316829407170616 1102 6 0.19959389764998292 1102 7 0.1493505894163647 1102 8 0.13800570388140443 1102 9 0.29459477646950205 1102 10-11 0.2913607298294423 1102 12-13 0.33059167262003797 1102 14-15 0.22888776435297586 1102 16-17 0.40214554496705546 1102 18-19 0.2797000274694028 1102 20-21 0.18152808137686804 1102 22-23 0.19692603040170553 1102 24-25 0.08910720027642327 1102 26-27 0.2392814976791584 1102 28-29 0.03961486248149981 1102 30-31 0.1553453281242625 1102 32-33 0.15743687965044728 1102 34-35 0.2279240586575142 1102 36-37 0.11525748113638201 1102 38-39 0.33752323687723873 1102 40-41 0.13640750455085993 1102 42-43 0.31127389746231415 1102 44-45 0.3001518744459162 1102 46-47 0.21506369976656714 1102 48-49 0.35317718659497643 1102 50-51 0.21141647858796375 1102 52-53 0.3322662917138359 1102 54-55 0.06939582634065289 1102 56-57 0.23373221705805491 1102 58-59 0.09141181213774274 1102 60-61 0.3052084422718906 1102 62-63 0.11191363576909197 1102 64-65 0.1352745460890361 1102 66-67 -0.1415998757083088 1102 68-69 0.14953912662358704 1102 70-71 0.20821733456013192 1102 72-73 0.14330006033478782 1102 74-75 0.29539587786275945 1102 76-77 0.20719382287476407 1102 78-79 0.18841811508582396 1102 80-81 0.234661477059813 1102 82-83 0.3510932802907618 1102 84-85 0.20671024275723582 1102 86-87 0.16384489068044772 1102 88-89 0.29391373995895265 1102 90-91 0.10663549469334654 1102 92-93 0.2666424238654663 1102 94-95 0.4531021988183994 1102 96-97 0.20485397966642793 1102 98-99 0.15847732761968203 1102 100-101 0.25618032924527157 1103 1 0.30054950909587674 1103 2 0.28456918387340835 1103 3 0.3002520722192301 1103 4 0.20443016269297942 1103 5 0.1859267772225195 1103 6 0.28301818261775935 1103 7 0.2653269946751422 1103 8 0.29042440985152496 1103 9 0.2734689500993355 1103 10-11 0.27845274340452164 1103 12-13 0.27484763247922217 1103 14-15 0.40113375234765414 1103 16-17 0.327448060610088 1103 18-19 0.3279333498576733 1103 20-21 0.20014316459873527 1103 22-23 0.26827448784903396 1103 24-25 0.3011089618450029 1103 26-27 0.38110098948335747 1103 28-29 0.33466490256196835 1103 30-31 0.21553952492696027 1103 32-33 0.18665924392286826 1103 34-35 0.36230244693580005 1103 36-37 0.3305657690835275 1103 38-39 0.40686520615913935 1103 40-41 0.33078260034510976 1103 42-43 0.41321474508872313 1103 44-45 0.5143952589282819 1103 46-47 0.33520231872189044 1103 48-49 0.25858818390989313 1103 50-51 0.303718892946776 1103 52-53 0.39901542982696725 1103 54-55 0.3820448209528635 1103 56-57 0.31514590572470524 1103 58-59 0.33407610096568163 1103 60-61 0.45058505673187454 1103 62-63 0.43210277732408997 1103 64-65 0.5243254532102171 1103 66-67 0.4025081315112544 1103 68-69 0.4823697486371614 1103 70-71 0.395826256176008 1103 72-73 0.2890289287570269 1103 74-75 0.3092951200835188 1103 76-77 0.2946360139362483 1103 78-79 0.4906812198146042 1103 80-81 0.3020294497949365 1103 82-83 0.3741317799458308 1103 84-85 0.281255203239958 1103 86-87 0.24560231826447065 1103 88-89 0.3036990950975742 1103 90-91 0.44092174122272 1103 92-93 0.3488776649771772 1103 94-95 0.41883579084975153 1103 96-97 0.48629264579809117 1103 98-99 0.3589117741731336 1103 100-101 0.5678953578812198 1104 1 0.2057690126983367 1104 2 0.16323402641986817 1104 3 0.22074390797540389 1104 4 0.26835316974296575 1104 5 0.27786929161462837 1104 6 0.1317135552854296 1104 7 0.1175873559286984 1104 8 0.15909814298060354 1104 9 0.22062214577481143 1104 10-11 0.2833805539809404 1104 12-13 0.1438167537688173 1104 14-15 0.03384933042622862 1104 16-17 0.30922162425270017 1104 18-19 0.2668391982761307 1104 20-21 0.2771899834366849 1104 22-23 0.1696126162500633 1104 24-25 0.046654135902905125 1104 26-27 0.14892552431562933 1104 28-29 0.23682916805901044 1104 30-31 0.3313095724042512 1104 32-33 0.08840665669802661 1104 34-35 0.14097257248577932 1104 36-37 0.1416250098061127 1104 38-39 0.23780694160502236 1104 40-41 0.2991455711798139 1104 42-43 0.26380806645219934 1104 44-45 0.2217721423153236 1104 46-47 0.15542616076839266 1104 48-49 0.18577555883738484 1104 50-51 0.017094730714639184 1104 52-53 0.13670176514543186 1104 54-55 0.26379257801365696 1104 56-57 0.2511918832888611 1104 58-59 0.19769714578053055 1104 60-61 0.307797862791773 1104 62-63 0.18219912535887062 1104 64-65 0.09016718481256447 1104 66-67 0.034757662607596274 1104 68-69 -0.07231000563194101 1104 70-71 0.04643459551579099 1104 72-73 0.050866900522933634 1104 74-75 0.07876206057598267 1104 76-77 -0.006300190679823459 1104 78-79 0.1435371358362154 1104 80-81 0.15888060755180433 1104 82-83 0.14307576244899423 1104 84-85 0.2441717090792359 1104 86-87 0.5566931679688061 1104 88-89 0.40779303699136094 1104 90-91 0.24784480570311374 1104 92-93 0.37411936609837326 1104 94-95 0.49322359684658323 1104 96-97 0.4007709098743639 1104 98-99 0.41286326047049116 1104 100-101 0.5284339185387843 1105 1 0.20041067153402992 1105 2 0.15145657511082788 1105 3 0.2078397426291616 1105 4 0.09284665980572271 1105 5 0.18896482218840305 1105 6 0.12244277938687986 1105 7 0.055458716662208474 1105 8 0.14053659395564466 1105 9 0.18752352799541683 1105 10-11 0.21381668588815472 1105 12-13 0.15638791184339595 1105 14-15 0.29517188296397734 1105 16-17 0.35272834118414664 1105 18-19 0.20634124898421646 1105 20-21 0.2825951120081669 1105 22-23 0.22552601024063534 1105 24-25 0.17308035040630187 1105 26-27 0.40493232520491773 1105 28-29 0.20917742878866363 1105 30-31 0.2679498060939878 1105 32-33 0.13153098389054207 1105 34-35 -0.035816507745536796 1105 36-37 -0.02212236689342717 1105 38-39 0.2960656593278159 1105 40-41 0.3120955317043723 1105 42-43 0.3151802579102423 1105 44-45 0.18222976132020108 1105 46-47 0.15057179554147382 1105 48-49 0.2759964106643409 1105 50-51 0.18884069325907404 1105 52-53 0.3196330473432596 1105 54-55 0.08613873216511081 1105 56-57 0.2052708445565159 1105 58-59 0.33176046952130633 1105 60-61 0.27004950790723115 1105 62-63 0.19213286278885278 1105 64-65 0.3366164556617335 1105 66-67 0.30901903217652205 1105 68-69 0.12876723434761317 1105 70-71 0.15424560431029022 1105 72-73 0.25877410972202597 1105 74-75 0.4195138311702209 1105 76-77 0.35497659720973473 1105 78-79 0.2985929693176175 1105 80-81 0.11904590066842502 1105 82-83 0.12319546493909428 1105 84-85 0.15324545885560426 1105 86-87 0.236806901820664 1105 88-89 0.4523032026050444 1105 90-91 0.4273993041024191 1105 92-93 0.3519933493690921 1105 94-95 0.5498352146559036 1105 96-97 0.5331688156303294 1105 98-99 0.4029445591240517 1105 100-101 0.6664609676135065 1106 1 0.30137574861151606 1106 2 0.30706560308519926 1106 3 0.4138077792763539 1106 4 0.3563615991988769 1106 5 0.3141443265248185 1106 6 0.3647414643085156 1106 7 0.25365659606617186 1106 8 0.3435489212068461 1106 9 0.44232703830243736 1106 10-11 0.3927696415975461 1106 12-13 0.3682750878991854 1106 14-15 0.4032511865643542 1106 16-17 0.435184204992467 1106 18-19 0.5532880668033968 1106 20-21 0.4427400022467367 1106 22-23 0.2805662861240066 1106 24-25 0.2841105380172806 1106 26-27 0.12944045758775502 1106 28-29 0.24488656742520476 1106 30-31 0.4969059787269785 1106 32-33 0.428889246237091 1106 34-35 0.5175927878768647 1106 36-37 0.31368649637445145 1106 38-39 0.20266903838435724 1106 40-41 0.3697717547787249 1106 42-43 0.40804222615277297 1106 44-45 0.33792017746143443 1106 46-47 0.5026940000348716 1106 48-49 0.3753182831712252 1106 50-51 0.4655398723001767 1106 52-53 0.36009390381699546 1106 54-55 0.5042554807560933 1106 56-57 0.43747321478581824 1106 58-59 0.45401804215943997 1106 60-61 0.5242189625743379 1106 62-63 0.4469846269497566 1106 64-65 0.23194033552893956 1106 66-67 0.23375363598020726 1106 68-69 0.4593305820069844 1106 70-71 0.539209381750652 1106 72-73 0.6256073057999529 1106 74-75 0.7467476629122558 1106 76-77 0.6506199175895873 1106 78-79 0.5723464114258618 1106 80-81 0.7276199671171959 1106 82-83 0.6049912160264128 1106 84-85 0.7278835549515428 1106 86-87 0.8981632854838395 1106 88-89 0.8402049963616385 1106 90-91 0.97951153613268 1106 92-93 0.940006770374314 1106 94-95 0.9887738228860776 1106 96-97 1.0769350610944706 1106 98-99 1.048496840566262 1106 100-101 1.2957320770661163 1107 1 0.2773340742891328 1107 2 0.3682722121808695 1107 3 0.3390226497416222 1107 4 0.2834618176420136 1107 5 0.2762335547604593 1107 6 0.32487048866390467 1107 7 0.33612993715730255 1107 8 0.36811935040336863 1107 9 0.43562539351410834 1107 10-11 0.4533627725477416 1107 12-13 0.443617723779667 1107 14-15 0.4458401970373771 1107 16-17 0.5459341080200559 1107 18-19 0.6342638645291387 1107 20-21 0.7302844904705381 1107 22-23 0.6397825788514098 1107 24-25 0.6702072450380783 1107 26-27 0.7155409924672043 1107 28-29 0.6252372333024852 1107 30-31 0.6145158297637323 1107 32-33 0.6181264986171726 1107 34-35 0.6127940141526551 1107 36-37 0.566318872232749 1107 38-39 0.6544654949781403 1107 40-41 0.814503430394069 1107 42-43 0.7165073228870114 1107 44-45 0.6865844249214419 1107 46-47 0.7789436682871198 1107 48-49 0.803730011049602 1107 50-51 0.7412110235158949 1107 52-53 0.7519785882993659 1107 54-55 0.7334219772957127 1107 56-57 0.8378668037180361 1107 58-59 0.8542950940618041 1107 60-61 0.7978872930835621 1107 62-63 0.8933440781879369 1107 64-65 0.8029984917600146 1107 66-67 0.7476461258916771 1107 68-69 0.8496648136111489 1107 70-71 0.7912465681214371 1107 72-73 0.8222153977229993 1107 74-75 0.7490501624188823 1107 76-77 0.8178084489848345 1107 78-79 0.8277706572526426 1107 80-81 0.8605552882149965 1107 82-83 0.9109641215922935 1107 84-85 0.9049876012533211 1107 86-87 0.9029513412038526 1107 88-89 0.8326622434337523 1107 90-91 0.8253221575047256 1107 92-93 0.9192474423081158 1107 94-95 0.9499874922543619 1107 96-97 0.8567634369255366 1107 98-99 0.9872004043877247 1107 100-101 1.041830298217672 1108 1 0.04878006819316383 1108 2 -0.02518554670231765 1108 3 -0.06367261037212302 1108 4 -0.1461604448955498 1108 5 -0.0184912298350568 1108 6 0.050464129943442515 1108 7 0.02076690078326493 1108 8 -0.006249826975647466 1108 9 -0.15251342672966928 1108 10-11 -0.20435666965945387 1108 12-13 0.058900029395758224 1108 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-2.5152076610014156 2316 66-67 -2.1895899367543805 2316 68-69 -2.296682479079962 2316 70-71 -2.070525355873137 2316 72-73 -2.603472879788548 2316 74-75 -2.8758346631219354 2316 76-77 -2.8810682731409756 2316 78-79 -3.138758866676067 2316 80-81 -3.191238810270214 2316 82-83 -3.6840922769562923 2316 84-85 -3.417533909961996 2316 86-87 -3.676132645173208 2316 88-89 -3.4959335896961328 2316 90-91 -3.946711263670121 2316 92-93 -4.143687074128234 2316 94-95 -4.310235440937895 2316 96-97 -4.660922745915961 2316 98-99 -5.064044111838463 2316 100-101 -5.055540255645262 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3857.0 3 1524.0 4 1442.0 5 1653.0 6 1892.0 7 2224.0 8 2792.0 9 3591.0 10 4419.0 11 5724.0 12 7030.0 13 8873.0 14 9643.0 15 11664.0 16 15056.0 17 19350.0 18 17122.0 19 11779.0 20 11921.0 21 12970.0 22 14174.0 23 15761.0 24 18549.0 25 21633.0 26 25551.0 27 30330.0 28 36985.0 29 44699.0 30 54832.0 31 69402.0 32 88097.0 33 115703.0 34 157874.0 35 225428.0 36 341866.0 37 510710.0 38 465624.0 39 77940.0 40 22.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.10072662214069 18.74793676819826 14.352923926035949 22.7984126836251 2 15.163523268383583 20.32517131173004 40.765974750479636 23.745330669406737 3 19.352147426109962 24.995322261741123 29.4830754974805 26.169454814668413 4 12.661611128665061 16.74314788258837 37.63260659974561 32.96263438900097 5 13.936830057518595 37.53420637957085 33.832662715370496 14.696300847540058 6 31.319939080919657 36.187911932445815 18.122250827867926 14.369898158766603 7 28.455657083498426 32.813660977698696 20.815375527795403 17.915306411007474 8 26.151094021091346 34.866382676577544 19.992243025916967 18.990280276414136 9 26.610631772295672 17.072516468675175 20.341736406378462 35.97511535265069 10-11 24.70215085134489 26.929181558111075 27.538474629841236 20.830192960702796 12-13 25.963046862131694 24.192783406360725 27.864190319782793 21.97997941172478 14-15 22.91293908483004 25.384325842514528 26.31622130167158 25.386513770983854 16-17 22.218949731778444 28.770309049811242 26.678742021363334 22.331999197046983 18-19 22.1253584951681 27.622638925550707 28.846744062659596 21.405258516621593 20-21 23.20165183919646 26.574832099612728 28.633189359873825 21.59032670131699 22-23 22.736745151361603 26.569582512620048 28.556326347600073 22.13734598841827 24-25 22.505488489206158 26.770929907897855 28.61337772042892 22.110203882467065 26-27 22.444236676365808 26.84776367672295 28.579275309620776 22.128724337290464 28-29 22.145613014935623 26.976143657988636 28.757716036884723 22.120527290191017 30-31 22.407968793118354 26.805107015008804 28.62440164505472 22.162522546818117 32-33 22.182005555690534 26.934530317387047 28.515108076807827 22.368356050114595 34-35 22.598980783909585 26.906150728170573 28.331251303377748 22.16361718454209 36-37 22.588244202662928 26.689110801651644 28.420587810113545 22.302057185571883 38-39 22.433459460192697 26.906687652491584 28.533365781187143 22.126487106128575 40-41 22.640297677643037 26.698965830160308 28.47522410252693 22.18551238966972 42-43 22.324283284742467 26.865403304178813 28.297801263362487 22.51251214771623 44-45 22.54657800354099 26.96826166487323 28.010292966911273 22.474867364674513 46-47 22.586944854027152 27.089437004244925 27.71347166172434 22.61014648000358 48-49 22.660195161474164 27.222067794915045 27.600049155797763 22.517687887813025 50-51 22.62844893524924 27.29923280285094 27.567247682758495 22.50507057914132 52-53 22.81515066969935 27.367757814103914 27.371624929490945 22.44546658670579 54-55 22.53759309436329 27.167558312677194 27.553582789038483 22.741265803921028 56-57 22.61843875823401 27.52916298674662 27.31760048560772 22.534797769411654 58-59 22.748421944113023 27.294085395717115 27.35091707130806 22.6065755888618 60-61 22.77433969720731 27.145112239884018 27.704545210675146 22.37600285223353 62-63 22.661881574018768 27.200842284291195 27.683212018748925 22.454064122941112 64-65 22.61939445008067 27.228670419306138 27.566573987822917 22.585361142790273 66-67 22.47449209987133 27.463968179975435 27.329933494982257 22.73160622517098 68-69 22.40541254382106 27.3968812612363 27.5426543874531 22.655051807489542 70-71 22.523754394600985 27.292338327808114 27.37851352843285 22.80539374915805 72-73 22.592079689018465 27.17077664399093 27.367002753482346 22.870140913508262 74-75 22.460149127558225 27.41930356519142 27.317475664265572 22.80307164298478 76-77 22.626995669375685 27.222932990544553 27.293321329246112 22.85675001083365 78-79 22.58538835643513 27.324780322323527 27.271116435648697 22.818714885592644 80-81 22.5229824801325 27.527515663541557 27.198796553767608 22.750705302558334 82-83 22.552063810100673 27.527107486451573 27.1671661576577 22.75366254579005 84-85 22.60443553906224 27.31591006463871 27.054350458286123 23.025303938012925 86-87 22.623839844757057 27.248603581061964 27.273837444907244 22.853719129273735 88-89 22.677170489909248 27.086775034859258 27.183210195025474 23.05284428020602 90-91 22.5419317466008 27.128305777172844 27.44988546116313 22.879877015063226 92-93 22.59866156450839 27.168466215147397 27.342793782053477 22.890078438290733 94-95 22.439048589021155 27.116933973438726 27.365761110559916 23.0782563269802 96-97 22.361862058268596 27.129408505849256 27.37069253538164 23.13803690050051 98-99 22.29131844297962 27.1235239923209 27.206763503990533 23.378394060708946 100-101 22.287594954969265 27.11241059650148 27.382203970355146 23.21779047817411 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 280.0 1 204.5 2 165.5 3 211.0 4 215.5 5 230.5 6 347.5 7 732.5 8 1244.0 9 1521.5 10 1509.5 11 1460.0 12 1563.0 13 1743.0 14 1929.0 15 2109.0 16 2258.5 17 2446.5 18 2688.5 19 2916.5 20 3168.0 21 3593.5 22 4287.0 23 5149.5 24 6450.0 25 8333.0 26 10878.5 27 14118.0 28 17384.0 29 21163.5 30 25843.0 31 30735.5 32 35503.0 33 40773.0 34 46989.0 35 51957.0 36 57417.5 37 64156.0 38 69154.0 39 72673.0 40 75888.5 41 79834.0 42 83716.5 43 87700.5 44 91566.5 45 94152.5 46 95476.5 47 98643.5 48 108215.0 49 108645.0 50 100258.5 51 95767.0 52 90600.0 53 86194.0 54 82107.5 55 75684.0 56 68940.5 57 61683.5 58 54844.5 59 48447.5 60 40519.5 61 32402.5 62 24904.0 63 18635.0 64 13786.5 65 10344.0 66 7603.0 67 5693.0 68 4051.5 69 2601.0 70 1769.5 71 1240.5 72 821.5 73 542.0 74 394.5 75 266.0 76 158.5 77 77.5 78 38.0 79 28.5 80 20.5 81 16.5 82 11.5 83 7.5 84 9.5 85 7.5 86 7.5 87 7.0 88 3.0 89 1.0 90 1.5 91 3.0 92 2.5 93 1.0 94 1.0 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03583422480246637 2 0.027088244511694913 3 0.022836726314792125 4 0.04559247133059562 5 0.09434321332174761 6 0.11446706612042082 7 0.05749672228192343 8 0.09106347071270832 9 0.07446230442004029 10-11 0.07474573896650047 12-13 0.07274145181653201 14-15 0.06551387088179726 16-17 0.05334643070875642 18-19 0.0638335089277833 20-21 0.05778015682838362 22-23 0.03308086063685313 24-25 0.008887697564001545 26-27 0.002915326763590484 28-29 0.007045373012010337 30-31 0.004960104563053254 32-33 0.0067214478160558385 34-35 0.006518994568584277 36-37 0.006761938465550151 38-39 0.004150291573167009 40-41 0.0076527327544250205 42-43 0.004292008846397102 44-45 0.0023687029954172685 46-47 0.0020852684489570824 48-49 3.0367987120734206E-4 50-51 0.0027128735161189225 52-53 0.006680957166561525 54-55 0.01226866679677662 56-57 0.00902941483723164 58-59 0.005365011057996377 60-61 0.0025306655933945176 62-63 0.009353340033186136 64-65 0.0026926281913717664 66-67 0.023039179562263687 68-69 0.046321303021493246 70-71 0.059865425277340706 72-73 0.004292008846397102 74-75 0.013908538101296266 76-77 0.022148385273388815 78-79 0.025671071779393984 80-81 0.07261997986804908 82-83 0.028039774774811257 84-85 0.011661307054361936 86-87 0.03245325556969129 88-89 0.04891270458912923 90-91 0.04498511158818094 92-93 0.04362867483012148 94-95 0.012997498487674241 96-97 0.016884600839128218 98-99 0.037514586756480325 100-101 0.07658806351849168 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2469706.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.19463358357615 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95718413319909 42.12191496821771 2 14.06469243601135 15.807204758147057 3 4.683552071545663 7.895714775903227 4 2.170739813641188 4.879357137313877 5 1.120740937345285 3.1489813158115982 6 0.7328565282820927 2.4709562445686344 7 0.45270430126907746 1.7807686632067272 8 0.31093402311499846 1.3978258798091627 9 0.22644295225152222 1.1452390856421768 >10 1.1703940013647207 11.559007346297394 >50 0.07216250399535616 2.760846542944521 >100 0.035575648840249045 3.588359081566292 >500 0.0014433208139580595 0.5082562903644366 >1k 4.329962441837673E-4 0.4393965456044431 >5k 1.443320813945891E-4 0.49617136460270983 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7057 0.28574251348136176 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5178 0.20966058308154897 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3272 0.13248540514538976 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.0490649494312276E-5 0.0 0.0 0.0 0.0 3 4.0490649494312276E-5 0.0 0.0 4.0490649494312276E-5 0.0 4 4.0490649494312276E-5 0.0 0.0 4.0490649494312276E-5 0.0 5 4.0490649494312276E-5 0.0 0.0 4.0490649494312276E-5 0.0 6 4.0490649494312276E-5 0.0 0.0 8.098129898862455E-5 0.0 7 4.0490649494312276E-5 0.0 0.0 8.098129898862455E-5 0.0 8 4.0490649494312276E-5 0.0 0.0 8.098129898862455E-5 0.0 9 4.0490649494312276E-5 0.0 0.0 1.619625979772491E-4 0.0 10-11 4.0490649494312276E-5 0.0 0.0 2.4294389696587367E-4 0.0 12-13 4.0490649494312276E-5 0.0 0.0 3.644158454488105E-4 0.0 14-15 4.0490649494312276E-5 0.0 0.0 4.049064949431228E-4 0.0 16-17 4.0490649494312276E-5 0.0 0.0 4.4539714443743504E-4 0.0 18-19 4.0490649494312276E-5 0.0 0.0 5.871144176675281E-4 0.0 20-21 4.0490649494312276E-5 0.0 0.0 6.883410414033087E-4 0.0 22-23 4.0490649494312276E-5 0.0 0.0 7.490770156447772E-4 0.0 24-25 8.098129898862455E-5 0.0 0.0 8.907942888748701E-4 0.0 26-27 8.098129898862455E-5 0.0 0.0 0.0011539835105879 0.0 28-29 2.024532474715614E-4 0.0 0.0 0.0019840418252213016 0.0 30-31 2.024532474715614E-4 0.0 0.0 0.0032190066347978262 0.0 32-33 2.024532474715614E-4 0.0 0.0 0.00435274482063857 0.0 34-35 2.2269857221871752E-4 0.0 0.0 0.0057496722281923434 0.0 36-37 2.4294389696587367E-4 0.0 0.0 0.007490770156447771 0.0 38-39 2.4294389696587367E-4 0.0 0.0 0.009312849383691823 0.0 40-41 2.4294389696587367E-4 0.0 0.0 0.012491365368995339 0.0 42-43 2.4294389696587367E-4 0.0 0.0 0.017390733957807124 0.0 44-45 2.8343454646018596E-4 0.0 0.0 0.02642014879503876 0.0 46-47 2.8343454646018596E-4 0.0 0.0 0.045511490031606996 0.0 48-49 2.8343454646018596E-4 0.0 0.0 0.06053352099399686 0.0 50-51 2.8343454646018596E-4 0.0 0.0 0.07836965209624142 0.0 52-53 3.2392519595449826E-4 0.0 0.0 0.1015505489317352 0.0 54-55 3.644158454488105E-4 0.0 0.0 0.1303596460469384 0.0 56-57 4.049064949431228E-4 0.0 0.0 0.16078836914191408 0.0 58-59 4.049064949431228E-4 0.0 0.0 0.19878884369232613 0.0 60-61 4.049064949431228E-4 0.0 0.0 0.24741811373499517 0.0 62-63 4.049064949431228E-4 0.0 0.0 0.3045301748467226 0.0 64-65 4.049064949431228E-4 4.0490649494312276E-5 0.0 0.3450005790162878 0.0 66-67 4.8588779393174734E-4 4.0490649494312276E-5 0.0 0.42294507929283887 0.0 68-69 4.8588779393174734E-4 4.0490649494312276E-5 0.0 0.5247183267967928 0.0 70-71 4.8588779393174734E-4 4.0490649494312276E-5 0.0 0.5886328170235648 0.0 72-73 5.061331186789035E-4 4.0490649494312276E-5 0.0 0.7043955839278035 0.0 74-75 5.263784434260596E-4 4.0490649494312276E-5 0.0 0.8000547433581162 0.0 76-77 5.263784434260596E-4 4.0490649494312276E-5 0.0 0.8759747111599518 0.0 78-79 5.263784434260596E-4 4.0490649494312276E-5 0.0 0.9692854129195945 0.0 80-81 5.668690929203719E-4 4.0490649494312276E-5 0.0 1.069175845222063 0.0 82-83 5.668690929203719E-4 4.0490649494312276E-5 0.0 1.1987864142533566 0.0 84-85 5.668690929203719E-4 4.0490649494312276E-5 0.0 1.3941740433881602 0.0 86-87 5.668690929203719E-4 4.0490649494312276E-5 0.0 1.672142352166614 0.0 88-89 5.668690929203719E-4 4.0490649494312276E-5 0.0 2.0032748837311 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12840 0.0 30.653023 1 GGTATCA 8625 0.0 27.556177 1 GTACATG 11440 0.0 26.17716 1 ATCAACG 15005 0.0 25.819464 3 TATCAAC 15110 0.0 25.734425 2 TCAACGC 15100 0.0 25.626581 4 CAACGCA 15360 0.0 25.192799 5 AACGCAG 15660 0.0 24.706173 6 TACATGG 11735 0.0 24.507437 2 ACATGGG 11870 0.0 23.507854 3 GAGTACT 6900 0.0 22.20996 12-13 ACGCAGA 17590 0.0 21.748217 7 CGCAGAG 17785 0.0 21.26971 8 GTACTTT 7490 0.0 20.01695 14-15 GCAGAGT 18525 0.0 19.982344 9 CATGGGA 7195 0.0 19.557085 4 CATGGGG 5465 0.0 19.311056 4 AGTACTT 7455 0.0 19.1542 12-13 CGTAGGG 150 7.684876E-4 19.01453 2 AGAGTAC 14750 0.0 18.314203 10-11 >>END_MODULE