FastQCFastQC Report
Thu 26 May 2016
SRR936840_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR936840_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2469706
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79150.32048349074748167No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61570.24930092893648068No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39280.15904727121365864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129800.030.3420431
GGTATCA88100.029.6586761
ATCAACG159100.024.569593
TCAACGC160950.024.3166894
TATCAAC161650.024.2407742
CAACGCA162300.024.1144285
GTACATG120400.024.069571
AACGCAG166750.023.4723196
TACATGG124600.022.8718592
ACATGGG121150.021.8765583
GAGTACT82000.021.26048712-13
ACGCAGA186250.020.9383057
CGCAGAG188650.020.5728788
AGTACTT86850.019.88179612-13
CCGTGCG2201.3449553E-619.4334379
GTACTTT89200.019.2777314-15
GCAGAGT200000.019.0253359
AGAGTAC163550.018.93768510-11
CATGGGA74650.018.833464
CATGGGG52850.018.6933274
Produced by FastQC (version 0.11.5)