##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936840_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2469706 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.193197894810154 33.0 31.0 34.0 31.0 34.0 2 32.32137549975584 33.0 31.0 34.0 31.0 34.0 3 32.45795086540665 34.0 31.0 34.0 31.0 34.0 4 36.10606525635035 37.0 35.0 37.0 35.0 37.0 5 35.95133955215722 37.0 35.0 37.0 35.0 37.0 6 36.03394817034902 37.0 35.0 37.0 35.0 37.0 7 35.992584947358104 37.0 35.0 37.0 35.0 37.0 8 35.938331526100676 37.0 35.0 37.0 35.0 37.0 9 37.654907102302865 39.0 37.0 39.0 35.0 39.0 10-11 37.56864319072797 39.0 37.0 39.0 35.0 39.0 12-13 37.47067161030503 39.0 37.0 39.0 35.0 39.0 14-15 38.704461786139724 40.0 38.0 41.0 34.5 41.0 16-17 38.6972738860415 40.0 38.0 41.0 34.5 41.0 18-19 38.80497881124312 40.0 38.0 41.0 35.0 41.0 20-21 38.80391410961467 40.0 38.0 41.0 35.0 41.0 22-23 38.71902809484206 40.0 38.0 41.0 34.0 41.0 24-25 38.63284637928563 40.0 38.0 41.0 34.0 41.0 26-27 38.442030144478736 40.0 38.0 41.0 34.0 41.0 28-29 38.30067060613692 40.0 38.0 41.0 34.0 41.0 30-31 38.319027851898156 40.0 38.0 41.0 34.0 41.0 32-33 38.223147815974855 40.0 38.0 41.0 34.0 41.0 34-35 38.12308995483673 40.0 38.0 41.0 34.0 41.0 36-37 37.99518201761667 40.0 37.5 41.0 33.0 41.0 38-39 37.84954423724929 40.0 37.0 41.0 33.0 41.0 40-41 37.546847479011674 40.0 37.0 41.0 32.5 41.0 42-43 37.373603781178815 40.0 37.0 41.0 32.0 41.0 44-45 37.17514858043832 40.0 37.0 41.0 32.0 41.0 46-47 36.853010236846 39.5 36.0 41.0 31.0 41.0 48-49 36.53959175707554 39.0 36.0 41.0 30.5 41.0 50-51 36.27631750499857 39.0 35.5 40.0 30.0 41.0 52-53 36.02315498281982 39.0 35.0 40.0 29.5 41.0 54-55 35.63799456291559 38.5 35.0 40.0 28.0 41.0 56-57 35.45854870984644 38.0 35.0 40.0 28.0 41.0 58-59 35.14498851280274 38.0 34.0 40.0 28.0 41.0 60-61 34.773992734357854 38.0 34.0 40.0 27.0 41.0 62-63 34.40234363928338 37.0 34.0 40.0 26.0 41.0 64-65 34.08646960407433 37.0 33.0 39.0 26.0 40.0 66-67 33.757009133880715 36.0 33.0 39.0 26.0 40.0 68-69 33.320316264365076 36.0 32.5 39.0 25.5 40.0 70-71 32.88143183844555 35.0 32.0 38.0 25.0 40.0 72-73 32.45979035561318 35.0 32.0 37.5 24.0 39.5 74-75 31.978296606964555 35.0 31.0 37.0 22.5 39.0 76-77 30.517124507937382 33.5 29.5 35.5 20.5 37.5 78-79 31.20747692235432 34.0 31.0 36.0 22.0 38.0 80-81 31.07793680705315 34.0 31.0 35.5 20.5 37.0 82-83 30.734174837004893 34.0 31.0 35.0 20.0 37.0 84-85 30.306583455682578 34.0 30.0 35.0 18.0 36.0 86-87 29.945689082020287 34.0 30.0 35.0 17.5 36.0 88-89 29.584810297258052 34.0 29.5 35.0 13.0 35.5 90-91 29.141200005182803 34.0 29.0 35.0 7.0 35.0 92-93 28.676100313154684 33.0 29.0 35.0 2.0 35.0 94-95 28.14557117324896 33.0 28.5 35.0 2.0 35.0 96-97 27.393836148918133 33.0 27.0 34.0 2.0 35.0 98-99 26.557101938449353 32.5 25.5 34.0 2.0 35.0 100-101 23.388869160944665 29.5 12.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.23842727447165402 1101 2 0.342044131735058 1101 3 0.26496152425316666 1101 4 0.16734587927270894 1101 5 0.05307088245383085 1101 6 0.18101480781973578 1101 7 0.11920921194181489 1101 8 0.18327889032901368 1101 9 0.21975879955137145 1101 10-11 0.307812388999011 1101 12-13 0.29845236797989116 1101 14-15 0.4304311459191439 1101 16-17 0.36806781723888804 1101 18-19 0.24457712983199542 1101 20-21 0.18202018843187773 1101 22-23 0.1494894301299894 1101 24-25 0.1727232326248398 1101 26-27 0.26191369946380405 1101 28-29 0.21988940575010218 1101 30-31 0.3145296640073383 1101 32-33 0.37268988397864433 1101 34-35 0.2214772881849214 1101 36-37 0.3721970368917411 1101 38-39 0.31687541314115464 1101 40-41 0.5493084831585548 1101 42-43 0.487383700838123 1101 44-45 0.2531595990460289 1101 46-47 0.12226202230027639 1101 48-49 0.2384376167369311 1101 50-51 0.24586116857838647 1101 52-53 0.318487918427806 1101 54-55 0.18931619111925357 1101 56-57 0.20887482647772515 1101 58-59 0.31593433787223546 1101 60-61 0.34736128726866156 1101 62-63 0.23091878088863638 1101 64-65 0.3296467707370354 1101 66-67 0.35416717222746996 1101 68-69 0.04202680955442872 1101 70-71 0.10420274869812118 1101 72-73 -0.027265149058116833 1101 74-75 0.0316755991262454 1101 76-77 0.14659219735073137 1101 78-79 0.19701717786418982 1101 80-81 0.1854823825861196 1101 82-83 0.4223268399977975 1101 84-85 0.26542679477736897 1101 86-87 -0.03115429937629699 1101 88-89 0.08488536675855585 1101 90-91 0.2332665697207048 1101 92-93 0.06648253423469441 1101 94-95 0.015153212183005138 1101 96-97 -0.052393323775181244 1101 98-99 0.16110907016760123 1101 100-101 0.6403506368401359 1102 1 -0.09520492746747777 1102 2 0.02587856475497574 1102 3 0.12239882438511529 1102 4 0.09798741078880369 1102 5 -0.06974527361352756 1102 6 0.0769417190087367 1102 7 0.15241655920138442 1102 8 0.17453385665127286 1102 9 0.23605175643797338 1102 10-11 0.2848420699289349 1102 12-13 0.22110234402502016 1102 14-15 0.22359496591906947 1102 16-17 0.26572927990807216 1102 18-19 0.2747369197763945 1102 20-21 0.1804305853228385 1102 22-23 0.1418850633445885 1102 24-25 0.09726117901595188 1102 26-27 0.2388895492127503 1102 28-29 -0.008800175478143046 1102 30-31 0.2185509640556944 1102 32-33 0.07447479927844824 1102 34-35 0.18815855787882185 1102 36-37 0.14714501855493722 1102 38-39 0.21636571102074953 1102 40-41 0.34361969894844435 1102 42-43 0.28703093244297406 1102 44-45 0.3764252454746 1102 46-47 0.3294668667265839 1102 48-49 0.44020282032487756 1102 50-51 0.3544629638166015 1102 52-53 0.5300564925443183 1102 54-55 0.6463850326500662 1102 56-57 0.439934642102763 1102 58-59 0.3670320823140827 1102 60-61 0.4486269216643848 1102 62-63 0.3357531850930471 1102 64-65 0.573728190714931 1102 66-67 0.4184821340342424 1102 68-69 0.40289537249304885 1102 70-71 0.06468480375728092 1102 72-73 0.20164308176111945 1102 74-75 0.25046119301558534 1102 76-77 0.2778633320265307 1102 78-79 0.09371387494125116 1102 80-81 0.24898132701999387 1102 82-83 0.49882989365070074 1102 84-85 0.27370587208559627 1102 86-87 0.1223080271883461 1102 88-89 0.03585790620651608 1102 90-91 0.032603005418884834 1102 92-93 0.029723120665245517 1102 94-95 0.025235475727168222 1102 96-97 0.22305140885959318 1102 98-99 0.14712847915823346 1102 100-101 0.04573536987389204 1103 1 0.10019757571670596 1103 2 0.19373086428019803 1103 3 0.16646194088556854 1103 4 0.13664915048119042 1103 5 0.08878185666397087 1103 6 0.032330651900323915 1103 7 0.09900257893178832 1103 8 0.11763363773992808 1103 9 0.12414270428795504 1103 10-11 0.17651956962492932 1103 12-13 0.23134863198829692 1103 14-15 0.22701278542820091 1103 16-17 0.18665252290325185 1103 18-19 0.13151199740522657 1103 20-21 0.14823863649198898 1103 22-23 0.13733974732139842 1103 24-25 0.13386070928424232 1103 26-27 0.1509347375102621 1103 28-29 0.08042076364466055 1103 30-31 0.23894979979510111 1103 32-33 0.2304841652302727 1103 34-35 0.193207456644501 1103 36-37 0.19681461987456572 1103 38-39 0.08777680633337326 1103 40-41 0.2583973950282683 1103 42-43 0.1497157499984212 1103 44-45 0.16669476009015227 1103 46-47 0.22060717166079513 1103 48-49 0.14335154947683293 1103 50-51 0.08781485596414029 1103 52-53 0.051113899781867644 1103 54-55 0.23992295014496534 1103 56-57 0.0958667004102054 1103 58-59 -0.027167962083659347 1103 60-61 -0.03192755415692261 1103 62-63 0.17639509100508377 1103 64-65 0.014746342101673804 1103 66-67 0.06710088918248402 1103 68-69 0.015493396345334531 1103 70-71 -0.023917076663401815 1103 72-73 0.11712409067144591 1103 74-75 -0.04405553635866255 1103 76-77 0.10162744795423606 1103 78-79 0.08592574598941027 1103 80-81 -0.03700317798849895 1103 82-83 0.20915800073829516 1103 84-85 -0.03609357022378035 1103 86-87 -0.24042071007232124 1103 88-89 -0.07121840980860838 1103 90-91 -0.10816480364811554 1103 92-93 -0.30176473852399255 1103 94-95 -0.12520639534788103 1103 96-97 -0.08537524388821538 1103 98-99 -0.10229077984164903 1103 100-101 0.5225447198114672 1104 1 0.02634919999542973 1104 2 0.0512185794420148 1104 3 -0.008978592602815638 1104 4 -0.03210511877183109 1104 5 -0.1490309145815374 1104 6 -0.025647640594350207 1104 7 2.255497279080032E-4 1104 8 -0.057986755306629334 1104 9 -0.03156774022485109 1104 10-11 0.05285425145974898 1104 12-13 0.08722124690141442 1104 14-15 0.10059147569149474 1104 16-17 0.08674135452992715 1104 18-19 0.14687173655543972 1104 20-21 0.1166574053414351 1104 22-23 7.179372130181605E-4 1104 24-25 0.021921803955216035 1104 26-27 -0.01098420073662254 1104 28-29 -0.05400926779662285 1104 30-31 0.04006423158061523 1104 32-33 -0.0028177965596540844 1104 34-35 0.005344296526907044 1104 36-37 -0.10377292134882055 1104 38-39 -0.01317233867334977 1104 40-41 0.10385440525488576 1104 42-43 0.10849622799449321 1104 44-45 0.22626611558568044 1104 46-47 0.014707748472773119 1104 48-49 0.08844177679586807 1104 50-51 0.13533665985460175 1104 52-53 0.14068047237842052 1104 54-55 0.22431085853708765 1104 56-57 0.10488216076386436 1104 58-59 -0.1551085063309685 1104 60-61 -0.09481436536122345 1104 62-63 0.021196919755333 1104 64-65 0.07501955055133891 1104 66-67 -0.0567815366836939 1104 68-69 0.106019368317817 1104 70-71 0.1336118488938851 1104 72-73 0.08212159534267016 1104 74-75 -0.07593356545999796 1104 76-77 0.08288116998603812 1104 78-79 0.1499466221636716 1104 80-81 0.06817815739749022 1104 82-83 0.20138241382406008 1104 84-85 0.0015297994094254364 1104 86-87 -0.05405986698212928 1104 88-89 0.09344628048050296 1104 90-91 0.01401919594467671 1104 92-93 -0.05006234257801623 1104 94-95 0.13443720076887544 1104 96-97 0.30599685452226666 1104 98-99 0.16721838015930857 1104 100-101 0.6741202494733791 1105 1 0.011751071003232028 1105 2 0.167976286923178 1105 3 0.1025592278031553 1105 4 0.12790262366631566 1105 5 0.06339398895855197 1105 6 0.07757759169405887 1105 7 0.11152401400186562 1105 8 0.13043613202440696 1105 9 0.08821489143475958 1105 10-11 0.10199148556532123 1105 12-13 0.13284973076960682 1105 14-15 0.14198428719183198 1105 16-17 0.019932524364698168 1105 18-19 0.0829936451519302 1105 20-21 0.11265574633477371 1105 22-23 0.1578178384786142 1105 24-25 0.062255296655770564 1105 26-27 0.009899163829025781 1105 28-29 0.04738911850341054 1105 30-31 0.07358920247014566 1105 32-33 0.057075122101387876 1105 34-35 0.04815469777204129 1105 36-37 0.17353619366240736 1105 38-39 0.13187854587578585 1105 40-41 0.24188175963080027 1105 42-43 0.21830520409368148 1105 44-45 0.16123581749570803 1105 46-47 0.17914075531701457 1105 48-49 0.16205538883254889 1105 50-51 0.1251306700895185 1105 52-53 0.33841863325627486 1105 54-55 0.3469902642011604 1105 56-57 0.15491339307548202 1105 58-59 0.13193513708457516 1105 60-61 0.07158668927120715 1105 62-63 0.13120624975966422 1105 64-65 0.09895165278815199 1105 66-67 -0.05371249648346321 1105 68-69 -0.02659698043872538 1105 70-71 -0.07029461720211572 1105 72-73 -0.05314179104590977 1105 74-75 -0.1993849251799027 1105 76-77 -0.18104804410153363 1105 78-79 -0.019418601710636807 1105 80-81 -0.04443058325342619 1105 82-83 -0.023022889990311768 1105 84-85 -0.2526103136863469 1105 86-87 -0.23568552864612968 1105 88-89 -0.1165420905972141 1105 90-91 2.090746313854197E-5 1105 92-93 0.07921454545032702 1105 94-95 0.15414752184192437 1105 96-97 0.11634680906292161 1105 98-99 0.5621620276658845 1105 100-101 0.6298967616759619 1106 1 0.011743350386616669 1106 2 0.09154861623321153 1106 3 0.0709748285958014 1106 4 0.10505924924214582 1106 5 0.07227848853391805 1106 6 0.1387383864028635 1106 7 0.06442889600511137 1106 8 0.2024604777021466 1106 9 0.27700326933320696 1106 10-11 0.21735744279310154 1106 12-13 0.24139516659841576 1106 14-15 0.3174735812701073 1106 16-17 0.24031430885656135 1106 18-19 0.15294371849778088 1106 20-21 0.199850460285937 1106 22-23 0.274195518175965 1106 24-25 0.167464139335344 1106 26-27 0.24774916511579903 1106 28-29 0.2884277344542028 1106 30-31 0.2968752255805285 1106 32-33 0.23170710604250644 1106 34-35 0.25563114989135016 1106 36-37 0.2744604158102888 1106 38-39 0.3664916786446568 1106 40-41 0.3750389127820952 1106 42-43 0.49701045668652455 1106 44-45 0.2722241851000007 1106 46-47 0.3169309218249836 1106 48-49 0.213902224666505 1106 50-51 0.35244042122830876 1106 52-53 0.31527286801448895 1106 54-55 0.5104016186546616 1106 56-57 0.47663599132032175 1106 58-59 0.4938708886866081 1106 60-61 0.32904834354199153 1106 62-63 0.34619325338835694 1106 64-65 0.3782848488660804 1106 66-67 0.42856965345090714 1106 68-69 0.3420003765368591 1106 70-71 0.43328163675470677 1106 72-73 0.35117371195226355 1106 74-75 0.38552255609760167 1106 76-77 0.3549872905871929 1106 78-79 0.34106245704325744 1106 80-81 0.31264632695580374 1106 82-83 0.454183597901185 1106 84-85 0.4825094845482063 1106 86-87 0.37551129560689844 1106 88-89 0.6813543630662195 1106 90-91 0.5273306586019331 1106 92-93 0.45061609472072917 1106 94-95 0.7145346464269373 1106 96-97 0.8977304722381554 1106 98-99 0.8334416555321624 1106 100-101 1.215580164923768 1107 1 0.22760973940593487 1107 2 0.21305335597284625 1107 3 0.2512256298942148 1107 4 0.11524730768419289 1107 5 0.0820211543567595 1107 6 0.05784178281930252 1107 7 0.10861140150021242 1107 8 0.22287027974231677 1107 9 0.2175234498039842 1107 10-11 0.22638234258826628 1107 12-13 0.25467018037870304 1107 14-15 0.24882110660202983 1107 16-17 0.29270663544605213 1107 18-19 0.34430449672696284 1107 20-21 0.2683929478156202 1107 22-23 0.13323853650205564 1107 24-25 0.20665834957154772 1107 26-27 0.2872299543692094 1107 28-29 0.26117329583944837 1107 30-31 0.2315527327428768 1107 32-33 0.2516240500162681 1107 34-35 0.3306570942666127 1107 36-37 0.36320863798260206 1107 38-39 0.31556814394894417 1107 40-41 0.34004981792605804 1107 42-43 0.355703438605417 1107 44-45 0.2964206750386822 1107 46-47 0.0894997212043549 1107 48-49 0.17295153769347138 1107 50-51 0.04229268289732602 1107 52-53 0.14898388340077418 1107 54-55 0.22035132909842758 1107 56-57 0.0898965443012898 1107 58-59 0.02450285964251009 1107 60-61 0.12440634189515976 1107 62-63 0.17314001723159578 1107 64-65 0.36807994784599884 1107 66-67 0.3555910180059598 1107 68-69 0.037754600791529924 1107 70-71 0.1075105358965871 1107 72-73 0.37392624854659573 1107 74-75 0.24661126714701354 1107 76-77 0.0536764616601495 1107 78-79 0.3710125587792348 1107 80-81 0.22265889934486438 1107 82-83 0.35266070560522067 1107 84-85 0.3666020949096236 1107 86-87 0.49612848252208863 1107 88-89 0.47838573109185845 1107 90-91 0.38864410748555756 1107 92-93 0.44470179792388365 1107 94-95 0.7236127109255044 1107 96-97 0.7535217671772152 1107 98-99 0.9766424428801521 1107 100-101 1.3894649821052383 1108 1 -0.029492678993030097 1108 2 0.03839317494569627 1108 3 -0.039367174686631756 1108 4 -0.0017048399451695673 1108 5 -0.14044920683635098 1108 6 -0.009676404513662362 1108 7 0.009362186487926749 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-1.0158588094957999 2316 78-79 -1.1769945892665028 2316 80-81 -1.125001101701205 2316 82-83 -1.036209747996086 2316 84-85 -1.0144291476882792 2316 86-87 -0.8832284935935206 2316 88-89 -1.5502981015665043 2316 90-91 -1.3944747418111803 2316 92-93 -1.2456609026645538 2316 94-95 -1.7156385393425282 2316 96-97 -1.6067936374175638 2316 98-99 -1.9627814406880475 2316 100-101 -3.1235550450957668 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 18.0 5 36.0 6 116.0 7 215.0 8 541.0 9 1016.0 10 1887.0 11 3093.0 12 4690.0 13 6294.0 14 8261.0 15 11231.0 16 15040.0 17 20721.0 18 18120.0 19 11716.0 20 10690.0 21 11065.0 22 11937.0 23 13469.0 24 15594.0 25 18850.0 26 23353.0 27 29160.0 28 37347.0 29 47393.0 30 59219.0 31 75770.0 32 97351.0 33 128112.0 34 176499.0 35 253057.0 36 393820.0 37 577600.0 38 367501.0 39 18916.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.17116031286842 19.257251373244184 14.807628364868439 22.763959949018954 2 15.528707585683014 20.7139461602688 39.99876904438162 23.758577209666566 3 19.50326740222189 24.822620084471833 29.846835586037002 25.827276927269278 4 13.140713914935622 17.11689569527709 37.236699429000865 32.505690960786424 5 13.937826540640149 37.58893506297884 33.84462263513007 14.628615761250934 6 31.572867377736458 36.274641597016 17.764584124588108 14.38790690065943 7 28.53876406700903 32.78390021124049 20.70460465393123 17.97273106781925 8 26.41253655293383 34.622056228555145 19.85556175512389 19.10984546338714 9 26.519534324761185 16.803916582715985 20.232222161036304 36.44432693148653 10-11 24.75517328783264 26.859735531273763 27.419559251182125 20.965531929711474 12-13 25.96736773245485 24.081720167990152 27.958054697830516 21.992857401724482 14-15 22.885924479016403 25.297660804860968 26.40393921117381 25.412475504948816 16-17 22.17974528142216 28.671489642896763 26.88125631147999 22.267508764201082 18-19 22.075298847933496 27.305059233082662 29.253358464667162 21.36628345431668 20-21 23.0350349742375 26.281899440209745 29.166469272272664 21.516596313280086 22-23 22.588909975903576 26.28891171703323 29.068787173624234 22.053391133438964 24-25 22.45530567064523 26.46515709951745 29.163995112751888 21.915542117085426 26-27 22.424363282989837 26.554075393772525 29.090982710450664 21.930578612786977 28-29 22.109266624070433 26.688037666082792 29.244580925148057 21.958114784698722 30-31 22.391491132952666 26.49704053842846 29.110428528739856 22.001039799879013 32-33 22.256400870873946 26.562789128895254 29.03924075081093 22.14156924941987 34-35 22.556778530881516 26.542334703469706 28.881965886215987 22.01892087943279 36-37 22.47759255881226 26.353531731803514 28.965774431657483 22.20310127772675 38-39 22.396892939057416 26.576592081222223 29.071437133119954 21.955077846600407 40-41 22.58922141674372 26.42874018928329 28.90529134389433 22.076747050078662 42-43 22.351587597876023 26.574802830782286 28.740424973660833 22.33318459768086 44-45 22.5545309715916 26.637558917177138 28.467030776727864 22.340879334503395 46-47 22.566668016358264 26.84846337611856 28.110580232416893 22.474288375106287 48-49 22.742527594911696 26.93562202912212 27.906710258944038 22.415140117022144 50-51 22.689450090518495 27.088130719155384 27.808432007469854 22.41398718285627 52-53 22.893863998158476 27.16263084361244 27.58671163579139 22.356793522437695 54-55 22.59357996457878 26.9609419096848 27.779824805057768 22.665653320678654 56-57 22.633588891257602 27.29438803058435 27.597583425804284 22.474439652353762 58-59 22.876775671853128 27.048397395796 27.60480123286279 22.470025699488076 60-61 22.83680336015293 26.872254544622624 28.024179139590455 22.266762955633986 62-63 22.678914008388045 26.980903799885493 27.90647955667598 22.433702635050487 64-65 22.58832989938675 27.016288790824976 27.825049515033072 22.5703317947552 66-67 22.518454123376248 27.24278259087976 27.606570904911536 22.632192380832453 68-69 22.517219695825528 27.09690363327383 27.780893314615817 22.60498335628483 70-71 22.598169243171913 27.054438838250483 27.647145045388307 22.700246873189297 72-73 22.653395397652183 26.99162164088212 27.586937825546144 22.76804513591956 74-75 22.539019501095407 27.178660937334552 27.49964012835118 22.782679433218856 76-77 22.627653584856308 27.035959608883907 27.560780118568985 22.7756066876908 78-79 22.607384102708934 27.02634634403606 27.532946848753742 22.83332270450126 80-81 22.558195893393183 27.269543168892653 27.44505354898833 22.72720738872583 82-83 22.591330782960224 27.280131546128604 27.388201177145117 22.740336493766055 84-85 22.596227918633634 27.08604416546592 27.37163182798172 22.946096087918725 86-87 22.570177637175735 26.985597828397573 27.487825429597233 22.95639910482946 88-89 22.637807510741908 26.84516568039311 27.436079492251835 23.080947316613145 90-91 22.49208042446131 26.83624117620501 27.650924424564156 23.02075397476952 92-93 22.496989520210096 26.816430781639593 27.617133375389617 23.069446322760683 94-95 22.353895564897197 26.79165050414908 27.616019882528526 23.238434048425198 96-97 22.29289885123653 26.67254187656067 27.664626337055648 23.36993293514716 98-99 22.1816750648209 26.74334162249951 27.486026077367214 23.588957235312375 100-101 22.035162465296203 26.8472401256777 27.532741981102184 23.584855427923912 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 366.0 1 275.0 2 213.5 3 239.0 4 223.0 5 239.0 6 362.5 7 743.0 8 1279.5 9 1583.5 10 1573.0 11 1513.5 12 1601.5 13 1775.5 14 2064.0 15 2340.0 16 2514.5 17 2750.5 18 2971.5 19 3266.0 20 3670.0 21 4165.5 22 4797.5 23 5683.0 24 6966.5 25 8761.5 26 11225.5 27 14362.0 28 17582.5 29 21212.5 30 25666.5 31 30410.5 32 35027.0 33 39762.5 34 45984.0 35 51475.0 36 56884.0 37 63579.5 38 68897.0 39 72427.0 40 75687.5 41 79486.0 42 83289.5 43 87127.5 44 90759.0 45 93552.0 46 95392.5 47 98935.0 48 108413.0 49 108724.0 50 100035.5 51 95702.0 52 91083.5 53 86668.0 54 82182.0 55 75781.5 56 68612.5 57 61372.0 58 55310.5 59 49073.0 60 40955.5 61 32607.0 62 25147.5 63 18816.0 64 13652.5 65 10272.0 66 7612.5 67 5621.0 68 4041.5 69 2540.5 70 1645.0 71 1115.5 72 751.5 73 531.0 74 387.5 75 268.0 76 157.5 77 66.0 78 30.0 79 18.5 80 14.0 81 8.0 82 2.5 83 2.0 84 1.5 85 1.0 86 1.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02239132917035469 2 0.003239251959544982 3 2.8343454646018596E-4 4 0.0 5 0.0027128735161189225 6 0.0 7 3.644158454488105E-4 8 0.0 9 8.098129898862455E-5 10-11 0.0 12-13 0.006336786645859872 14-15 0.0030165533873262648 16-17 0.0 18-19 5.466237681732158E-4 20-21 0.0012552101343236805 22-23 0.0018220792272440527 24-25 5.466237681732158E-4 26-27 2.429438969658737E-4 28-29 1.619625979772491E-4 30-31 0.0 32-33 1.2147194848293685E-4 34-35 2.0245324747156138E-5 36-37 0.001882815201485521 38-39 0.009474811981669074 40-41 8.705489641277139E-4 42-43 0.0 44-45 0.0012754554590708367 46-47 2.429438969658737E-4 48-49 1.0122662373578069E-4 50-51 0.0018220792272440527 52-53 4.6564246918459124E-4 54-55 0.0 56-57 4.6564246918459124E-4 58-59 2.8343454646018596E-4 60-61 0.0022877216964286434 62-63 0.0 64-65 6.0735974241468424E-5 66-67 2.0245324747156138E-5 68-69 2.0245324747156137E-4 70-71 6.883410414033087E-4 72-73 4.0490649494312274E-4 74-75 0.003057044036820577 76-77 1.8220792272440525E-4 78-79 3.4417052070165435E-4 80-81 0.0020852684489570824 82-83 8.098129898862455E-5 84-85 0.0024901749439002053 86-87 3.644158454488105E-4 88-89 0.0022877216964286434 90-91 0.0013969274075537736 92-93 0.0 94-95 0.0 96-97 2.8343454646018596E-4 98-99 6.680957166561526E-4 100-101 0.002247231046934331 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2469706.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.40715866203658 #Duplication Level Percentage of deduplicated Percentage of total 1 73.61791364619879 40.05341508116845 2 14.660344205513177 15.952553464588478 3 5.004391165033328 8.168241123685872 4 2.264381408777626 4.927942343149208 5 1.2243510123923005 3.3306729894626503 6 0.7597966245189622 2.480302530064981 7 0.4929805923847158 1.8775171315026014 8 0.34381802329233185 1.496492939530695 9 0.24735807347092773 1.2112244954701619 >10 1.2667191752511509 12.213751862954519 >50 0.07782858912783226 2.88166637354821 >100 0.03765618114011792 3.725009130260884 >500 0.0019392083444539241 0.6862812590256492 >1k 3.72924681624335E-4 0.42389392335382864 >5k 1.49169872649734E-4 0.5710353522338767 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7915 0.32048349074748167 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6157 0.24930092893648068 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3928 0.15904727121365864 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.2147194848293683E-4 0.0 10-11 0.0 0.0 0.0 1.8220792272440525E-4 0.0 12-13 0.0 0.0 0.0 2.8343454646018596E-4 0.0 14-15 0.0 0.0 0.0 2.8343454646018596E-4 0.0 16-17 0.0 0.0 0.0 3.4417052070165435E-4 0.0 18-19 0.0 0.0 0.0 4.0490649494312274E-4 0.0 20-21 0.0 0.0 0.0 4.4539714443743504E-4 0.0 22-23 0.0 0.0 0.0 5.061331186789035E-4 0.0 24-25 4.0490649494312276E-5 0.0 0.0 6.883410414033087E-4 0.0 26-27 4.0490649494312276E-5 0.0 0.0 9.110396136220262E-4 0.0 28-29 1.2147194848293683E-4 0.0 0.0 0.0017006072787611156 0.0 30-31 1.2147194848293683E-4 0.0 0.0 0.00283434546460186 0.0 32-33 1.2147194848293683E-4 0.0 0.0 0.003927593000948291 0.0 34-35 1.2147194848293683E-4 0.0 0.0 0.005324520408502065 0.0 36-37 1.619625979772491E-4 0.0 0.0 0.007045373012010336 0.0 38-39 1.619625979772491E-4 0.0 0.0 0.008867452239254388 0.0 40-41 1.8220792272440525E-4 0.0 0.0 0.012126949523546527 0.0 42-43 2.2269857221871752E-4 0.0 0.0 0.016803619540139595 0.0 44-45 2.4294389696587367E-4 0.0 0.0 0.025873525026865546 0.0 46-47 2.4294389696587367E-4 0.0 0.0 0.044904130289192316 0.0 48-49 2.4294389696587367E-4 0.0 0.0 0.059865425277340706 0.0 50-51 2.4294389696587367E-4 0.0 0.0 0.07753959378160802 0.0 52-53 2.4294389696587367E-4 0.0 0.0 0.10045730139538876 0.0 54-55 2.4294389696587367E-4 0.0 0.0 0.12914492656210902 0.0 56-57 2.4294389696587367E-4 0.0 0.0 0.1595129136828432 0.0 58-59 2.4294389696587367E-4 0.0 0.0 0.19749314290850814 0.0 60-61 2.4294389696587367E-4 0.0 0.0 0.24587946905421132 0.0 62-63 2.4294389696587367E-4 0.0 0.0 0.3030725114649274 0.0 64-65 2.4294389696587367E-4 4.0490649494312276E-5 0.0 0.34376561420671126 0.0 66-67 2.4294389696587367E-4 4.0490649494312276E-5 0.0 0.4216088878595266 0.0 68-69 2.4294389696587367E-4 4.0490649494312276E-5 0.0 0.5236453245851935 0.0 70-71 3.239251959544982E-4 4.0490649494312276E-5 0.0 0.5875193241624712 0.0 72-73 3.239251959544982E-4 4.0490649494312276E-5 0.0 0.7035047896389287 0.0 74-75 3.239251959544982E-4 4.0490649494312276E-5 0.0 0.7991639490692415 0.0 76-77 3.239251959544982E-4 4.0490649494312276E-5 0.0 0.8752661247938014 0.0 78-79 3.239251959544982E-4 4.0490649494312276E-5 0.0 0.9693056582443416 0.0 80-81 3.644158454488105E-4 4.0490649494312276E-5 0.0 1.0691555998973157 0.0 82-83 3.644158454488105E-4 4.0490649494312276E-5 0.0 1.1983612624336661 0.0 84-85 3.644158454488105E-4 4.0490649494312276E-5 0.0 1.3946801765068393 0.0 86-87 3.644158454488105E-4 4.0490649494312276E-5 0.0 1.6718791629449012 0.0 88-89 3.644158454488105E-4 4.0490649494312276E-5 0.0 2.0027282599629266 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 12980 0.0 30.342043 1 GGTATCA 8810 0.0 29.658676 1 ATCAACG 15910 0.0 24.56959 3 TCAACGC 16095 0.0 24.316689 4 TATCAAC 16165 0.0 24.240774 2 CAACGCA 16230 0.0 24.114428 5 GTACATG 12040 0.0 24.06957 1 AACGCAG 16675 0.0 23.472319 6 TACATGG 12460 0.0 22.871859 2 ACATGGG 12115 0.0 21.876558 3 GAGTACT 8200 0.0 21.260487 12-13 ACGCAGA 18625 0.0 20.938305 7 CGCAGAG 18865 0.0 20.572878 8 AGTACTT 8685 0.0 19.881796 12-13 CCGTGCG 220 1.3449553E-6 19.433437 9 GTACTTT 8920 0.0 19.27773 14-15 GCAGAGT 20000 0.0 19.025335 9 AGAGTAC 16355 0.0 18.937685 10-11 CATGGGA 7465 0.0 18.83346 4 CATGGGG 5285 0.0 18.693327 4 >>END_MODULE