##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR936839_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 425347 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.944621685353372 33.0 31.0 34.0 30.0 34.0 2 32.060444766273186 33.0 31.0 34.0 30.0 34.0 3 32.027544569492676 33.0 31.0 34.0 30.0 34.0 4 35.64846113878786 37.0 35.0 37.0 33.0 37.0 5 35.6924628597357 37.0 35.0 37.0 35.0 37.0 6 35.781312669420494 37.0 35.0 37.0 35.0 37.0 7 35.75044610635552 37.0 35.0 37.0 35.0 37.0 8 35.74330135160234 37.0 35.0 37.0 35.0 37.0 9 37.36011303712028 39.0 37.0 39.0 34.0 39.0 10-11 37.266290816674385 39.0 37.0 39.0 34.0 39.0 12-13 37.202841444749815 39.0 37.0 39.0 34.0 39.0 14-15 38.31117181971426 40.0 38.0 41.0 33.5 41.0 16-17 38.23843826334734 40.0 38.0 41.0 33.5 41.0 18-19 38.25617437057274 40.0 38.0 41.0 34.0 41.0 20-21 38.23509040853703 40.0 38.0 41.0 34.0 41.0 22-23 38.11117511114455 40.0 38.0 41.0 33.5 41.0 24-25 37.9558266544727 40.0 37.0 41.0 33.0 41.0 26-27 37.8851772787865 40.0 37.0 41.0 33.0 41.0 28-29 37.934980145622276 40.0 37.0 41.0 33.0 41.0 30-31 37.77069310468865 40.0 37.0 41.0 33.0 41.0 32-33 37.52445767808401 40.0 36.0 41.0 33.0 41.0 34-35 37.332265185836505 39.0 36.0 41.0 33.0 41.0 36-37 37.14492637775746 39.0 35.0 41.0 33.0 41.0 38-39 36.97218976506241 39.0 35.0 41.0 32.0 41.0 40-41 36.83044079304662 39.0 35.0 41.0 31.5 41.0 42-43 36.60445236477511 39.0 35.0 41.0 31.0 41.0 44-45 36.30645919684399 39.0 35.0 41.0 30.5 41.0 46-47 35.873894725953164 39.0 35.0 41.0 29.5 41.0 48-49 35.4882554714151 39.0 35.0 40.0 27.5 41.0 50-51 34.82581280695526 38.5 34.5 39.5 26.5 40.5 52-53 35.12776744634381 38.5 35.0 40.0 27.5 40.5 54-55 35.47864684598692 39.0 35.0 41.0 27.5 41.0 56-57 35.41042372462954 39.0 35.0 41.0 27.0 41.0 58-59 35.230685769501136 39.0 35.0 41.0 26.5 41.0 60-61 34.99198654275215 39.0 35.0 41.0 26.0 41.0 62-63 34.71751417078292 38.5 35.0 40.0 26.0 41.0 64-65 34.45385179629808 38.0 35.0 40.0 25.5 41.0 66-67 34.08398789694061 37.5 34.0 40.0 23.0 41.0 68-69 33.63853865197122 37.0 34.0 39.5 22.0 41.0 70-71 33.274846184409434 36.5 34.0 39.0 21.5 41.0 72-73 32.87920921036236 36.0 33.0 39.0 20.5 40.0 74-75 32.42186026938006 35.5 33.0 38.0 19.0 40.0 76-77 31.972018140482945 35.0 32.5 37.0 15.5 39.0 78-79 31.507105962896176 35.0 32.0 37.0 9.5 39.0 80-81 31.031250955102543 35.0 32.0 36.0 6.5 39.0 82-83 30.578885004478696 35.0 31.0 36.0 2.0 37.5 84-85 30.183450218292357 35.0 31.0 35.5 2.0 37.0 86-87 29.89181538837702 34.5 31.0 35.0 2.0 36.5 88-89 29.46336285432835 34.0 30.5 35.0 2.0 36.0 90-91 29.110034865650867 34.0 30.0 35.0 2.0 36.0 92-93 28.78076840791166 34.0 29.5 35.0 2.0 35.5 94-95 28.381148803212437 34.0 29.0 35.0 2.0 35.0 96-97 27.96268693560787 34.0 29.0 35.0 2.0 35.0 98-99 27.456568401799004 34.0 29.0 35.0 2.0 35.0 100-101 25.364805676306638 32.0 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2930882089862088 1101 2 -0.0018347164073517774 1101 3 0.25021098251487217 1101 4 0.04911198706611941 1101 5 0.09776117907771464 1101 6 0.06091611766871807 1101 7 0.16109362437116204 1101 8 0.22664791679216734 1101 9 0.22657233916488195 1101 10-11 0.3626022943496636 1101 12-13 0.39355528175037335 1101 14-15 0.6339036212801616 1101 16-17 0.6525433664049203 1101 18-19 0.45676026328125374 1101 20-21 0.6163581112165843 1101 22-23 0.7126107919249094 1101 24-25 0.6773774049361094 1101 26-27 0.7861957321261954 1101 28-29 0.5030445144358211 1101 30-31 0.4852013674288784 1101 32-33 0.5169132794976221 1101 34-35 0.6749166147805425 1101 36-37 0.6742548791930716 1101 38-39 0.8775190648751305 1101 40-41 0.9489870409591674 1101 42-43 0.6150000413183463 1101 44-45 1.0115360803198143 1101 46-47 0.8990583714874134 1101 48-49 0.560049829240711 1101 50-51 0.7652234868784618 1101 52-53 0.7723221277212318 1101 54-55 0.8506493656169667 1101 56-57 0.9128071871242156 1101 58-59 0.6721272437029526 1101 60-61 0.9157865031789214 1101 62-63 1.0528120024641368 1101 64-65 1.0043698321645635 1101 66-67 1.097577232807339 1101 68-69 1.0965145277963089 1101 70-71 0.9645873915689478 1101 72-73 0.9425148248324859 1101 74-75 0.7255893214238611 1101 76-77 0.697698096844718 1101 78-79 0.5575126058646802 1101 80-81 0.6556115499445561 1101 82-83 0.39461160854544985 1101 84-85 0.20022552214282285 1101 86-87 0.3265168433879069 1101 88-89 0.6788389711219729 1101 90-91 0.8441534526265322 1101 92-93 0.7634016423545411 1101 94-95 0.9821627980857315 1101 96-97 1.1223053673614949 1101 98-99 1.3227519964593846 1101 100-101 2.073596877480142 1102 1 0.5249632089862111 1102 2 0.41243611692598137 1102 3 0.4640651491815362 1102 4 0.37598698706612055 1102 5 0.3413028457443801 1102 6 0.24893695100205093 1102 7 0.06255195770449973 1102 8 0.2655020834588342 1102 9 0.23303067249821652 1102 10-11 0.48676896101633105 1102 12-13 0.36704486508370593 1102 14-15 0.7023932046134931 1102 16-17 0.5773871164049211 1102 18-19 0.5291040132812554 1102 20-21 0.4014622778832546 1102 22-23 0.6531837085915697 1102 24-25 0.7549294882694397 1102 26-27 0.7065082321261968 1102 28-29 0.8124195144358239 1102 30-31 0.7382742840955459 1102 32-33 0.899517446164289 1102 34-35 0.6062186981138709 1102 36-37 0.5460257125264079 1102 38-39 0.508039898208466 1102 40-41 0.5845599576258422 1102 42-43 0.775729207985016 1102 44-45 1.0053381636531427 1102 46-47 0.8090062881540732 1102 48-49 0.8952060792407082 1102 50-51 0.5270464035451354 1102 52-53 0.6341971277212295 1102 54-55 0.6409097822836287 1102 56-57 0.6791613537908887 1102 58-59 0.6292626603696263 1102 60-61 0.6189635865122511 1102 62-63 0.8724995024641373 1102 64-65 0.9033281654978893 1102 66-67 0.6967438994740078 1102 68-69 0.6452124444629774 1102 70-71 0.772139474902275 1102 72-73 0.8407439914991457 1102 74-75 0.9984539047571914 1102 76-77 1.064416846844722 1102 78-79 1.041939689198017 1102 80-81 1.0512365499445586 1102 82-83 1.0911220252121154 1102 84-85 1.0237151054761569 1102 86-87 0.7938085100545749 1102 88-89 0.9224327211219716 1102 90-91 0.7554034526265347 1102 92-93 0.6395995590212067 1102 94-95 0.9208086314190638 1102 96-97 0.6368887006948292 1102 98-99 0.767699913126048 1102 100-101 1.6276593774801427 1103 1 0.4369870185100204 1103 2 0.5507694502593168 1103 3 0.678708006324392 1103 4 0.5412250823042157 1103 5 0.5827314171729512 1103 6 0.4584607605258668 1103 7 0.3767186243711649 1103 8 0.40812113107787695 1103 9 0.2070782915458338 1103 10-11 0.4917689610163265 1103 12-13 0.3563901031789385 1103 14-15 0.4601312998515894 1103 16-17 0.6438752116430138 1103 18-19 0.6519611561383982 1103 20-21 0.5549741826451609 1103 22-23 0.45699323240110346 1103 24-25 0.5989175835075358 1103 26-27 0.6338891845071473 1103 28-29 0.7011099906262999 1103 30-31 0.557559998381258 1103 32-33 0.3379698271166731 1103 34-35 0.6711591743043499 1103 36-37 0.7268590458597401 1103 38-39 0.7357779934465682 1103 40-41 0.5270004338163119 1103 42-43 0.6713244460802557 1103 44-45 0.8893262588912378 1103 46-47 0.9891253357731244 1103 48-49 0.82883703162166 1103 50-51 0.7450225940213215 1103 52-53 0.919494746768855 1103 54-55 1.2047788299026791 1103 56-57 0.8808875442670754 1103 58-59 0.896941231798202 1103 60-61 1.0484873960360588 1103 62-63 1.0860709310355645 1103 64-65 1.2429114988312264 1103 66-67 1.263351042331152 1103 68-69 1.2190219682725072 1103 70-71 1.1718418558546588 1103 72-73 1.163422562927721 1103 74-75 1.170418190471473 1103 76-77 0.8019763706542449 1103 78-79 1.1678920701504012 1103 80-81 1.1873079785159852 1103 82-83 1.3323720252121127 1103 84-85 0.9818103435713965 1103 86-87 0.9347013671974302 1103 88-89 0.8939803401695912 1103 90-91 1.0673677383408169 1103 92-93 0.933230511402158 1103 94-95 0.7143800599904928 1103 96-97 0.7041506054567357 1103 98-99 0.7654975321736686 1103 100-101 1.2620641393849041 1104 1 0.4419831153093945 1104 2 0.4935485291039683 1104 3 0.5102068353642082 1104 4 0.3717481111878982 1104 5 0.37921853661089244 1104 6 0.536173484959896 1104 7 0.4497475080557862 1104 8 0.461907235683654 1104 9 0.5630892205075853 1104 10-11 0.584450459845371 1104 12-13 0.6363861999783111 1104 14-15 0.8069892233488574 1104 16-17 0.8368689665220117 1104 18-19 0.7926520226489373 1104 20-21 0.873572933620963 1104 22-23 0.84337106222155 1104 24-25 0.6520401440071453 1104 26-27 0.786836100978654 1104 28-29 0.7618633083468254 1104 30-31 0.8459089445170989 1104 32-33 0.7333640503797483 1104 34-35 0.8826736161466613 1104 36-37 0.8599191551493632 1104 38-39 1.053010624203786 1104 40-41 1.0360558592651827 1104 42-43 1.102198763020148 1104 44-45 0.8517579528803125 1104 46-47 1.0321971546646225 1104 48-49 1.259067320458513 1104 50-51 1.2142653731001687 1104 52-53 1.2555993525455875 1104 54-55 1.1554618900119635 1104 56-57 1.3916994099969742 1104 58-59 1.231367461306398 1104 60-61 1.3773125326480837 1104 62-63 1.4397594556257332 1104 64-65 1.530655448870256 1104 66-67 1.6944985833147612 1104 68-69 1.7913482758447188 1104 70-71 1.4336880697500547 1104 72-73 1.2188675277989134 1104 74-75 1.1319521483169055 1104 76-77 1.2135883925121647 1104 78-79 1.11615514587249 1104 80-81 0.8057506014668014 1104 82-83 0.9979457957039166 1104 84-85 0.9908462530171427 1104 86-87 0.9807786505697997 1104 88-89 0.9667945478198625 1104 90-91 0.8258571996991328 1104 92-93 1.036470167920502 1104 94-95 1.0081739709975182 1104 96-97 1.1571170379313642 1104 98-99 1.3302321145312028 1104 100-101 1.3308970823981738 1105 1 0.18312358634470272 1105 2 0.2311153622090032 1105 3 0.1343009982381389 1105 4 0.18089264744347844 1105 5 0.1603594495179621 1105 6 -0.02983663390360647 1105 7 0.10675007091204236 1105 8 0.09540774383619066 1105 9 0.2300118045736923 1105 10-11 0.22856141384651352 1105 12-13 0.025606185838420004 1105 14-15 0.09555358197197705 1105 16-17 0.285429569235113 1105 18-19 0.2824530698850225 1105 20-21 0.32591982505306305 1105 22-23 0.26290068972365077 1105 24-25 0.2895285448732068 1105 26-27 0.04400823212619542 1105 28-29 0.42256102386978256 1105 30-31 0.13926485013328715 1105 32-33 0.08083820088127425 1105 34-35 -0.23474828301820594 1105 36-37 -0.18713466483207952 1105 38-39 0.11341725669903013 1105 40-41 0.22864014630508223 1105 42-43 -0.06488399956214863 1105 44-45 -0.27194957219591487 1105 46-47 -0.484908806185544 1105 48-49 -0.3417042981177758 1105 50-51 -0.11903850211524514 1105 52-53 -0.06936419303348629 1105 54-55 -0.15581191582957388 1105 56-57 0.2550339953003231 1105 58-59 0.19485228301113722 1105 60-61 -0.036036413487749996 1105 62-63 0.011980634539611401 1105 64-65 0.015474391912981389 1105 66-67 0.10209767305892115 1105 68-69 0.36997659540637784 1105 70-71 0.3125875881098281 1105 72-73 0.51322040659349 1105 74-75 0.4068737160779463 1105 76-77 0.11566684684471795 1105 78-79 0.17349629297159908 1105 80-81 0.32335919145399217 1105 82-83 0.5102022138913576 1105 84-85 0.6665924639667224 1105 86-87 0.6084547364696711 1105 88-89 0.32115913621631265 1105 90-91 0.07292703753219243 1105 92-93 0.03863257788912833 1105 94-95 0.2843935370794419 1105 96-97 0.44089813465709327 1105 98-99 0.273100856522273 1105 100-101 0.4640744718197638 1106 1 0.3754013533161036 1106 2 0.2834154983692798 1106 3 0.3452764893877216 1106 4 0.24547152314859488 1106 5 0.10253995914644065 1106 6 0.32522561079586865 1106 7 0.31417566904470107 1106 8 0.29658455768563385 1106 9 0.3545255178590452 1106 10-11 0.35465555895447665 1106 12-13 0.5381402259084425 1106 14-15 0.7581921736856501 1106 16-17 0.6937402091884266 1106 18-19 0.7637431885389816 1106 20-21 0.7365524840688238 1106 22-23 0.5713280384884811 1106 24-25 0.8410506222900551 1106 26-27 0.8861474073839233 1106 28-29 0.801208174229636 1106 30-31 0.8767794387347223 1106 32-33 0.770161776061201 1106 34-35 0.48775220326851354 1106 36-37 0.7043504547944508 1106 38-39 0.5413130940847495 1106 40-41 0.4446243906155303 1106 42-43 0.6138993110777946 1106 44-45 0.5350675450964388 1106 46-47 0.4309392778447929 1106 48-49 0.6560952544984389 1106 50-51 0.6893402179781205 1106 52-53 0.8427151689583425 1106 54-55 0.9074175142423968 1106 56-57 1.184741250698103 1106 58-59 1.2396879181015876 1106 60-61 1.33896358651225 1106 62-63 1.210592285969291 1106 64-65 0.8716915675597434 1106 66-67 1.1975557551441085 1106 68-69 0.7988206918856591 1106 70-71 0.6630544233558808 1106 72-73 0.6595197646950268 1106 74-75 0.943466791355128 1106 76-77 0.6794555066385328 1106 78-79 0.9513726788887382 1106 80-81 1.0662107767486795 1106 82-83 1.1926813035626296 1106 84-85 1.0403645900122385 1106 86-87 0.9865791286112753 1106 88-89 1.1336956077199112 1106 90-91 1.1347462361316865 1106 92-93 1.4228598682995575 1106 94-95 1.147406569563394 1106 96-97 0.5828938553340066 1106 98-99 0.9180993976621323 1106 100-101 1.695777934181173 1107 1 0.19830603358757415 1107 2 0.49374591191459416 1107 3 0.3679205022567089 1107 4 0.31409632647386587 1107 5 0.31130284574437894 1107 6 0.32877749769909315 1107 7 0.408115739025682 1107 8 0.33111711762739304 1107 9 0.45352042192874364 1107 10-11 0.4605047241142799 1107 12-13 0.5005841589333571 1107 14-15 0.4347679198754477 1107 16-17 0.6164617177716636 1107 18-19 0.5222589107755624 1107 20-21 0.6328432573821203 1107 22-23 0.7829559181587769 1107 24-25 0.6379619484061152 1107 26-27 0.7465993482993198 1107 28-29 0.578393318536051 1107 30-31 0.651076106419012 1107 32-33 0.6825299746380935 1107 34-35 0.6859709760181971 1107 36-37 0.6578765097929207 1107 38-39 0.6761321533337465 1107 40-41 0.34443182550738527 1107 42-43 0.4048692991011862 1107 44-45 0.2761496670700012 1107 46-47 0.3249345341677454 1107 48-49 -0.12496761096430475 1107 50-51 -0.2247246670698999 1107 52-53 -0.23497428457945801 1107 54-55 0.044802151304132565 1107 56-57 0.24997855196856733 1107 58-59 0.07858783121245239 1107 60-61 0.061913472617035836 1107 62-63 0.06621248651880762 1107 64-65 0.21117269852750553 1107 66-67 0.33962829582936394 1107 68-69 0.43291176109168106 1107 70-71 0.36395325622346064 1107 72-73 0.09193704389095103 1107 74-75 0.362551285167207 1107 76-77 0.18528529786977543 1107 78-79 0.3684317165328679 1107 80-81 0.34022857727940803 1107 82-83 0.5382945764649598 1107 84-85 0.6934303674351554 1107 86-87 0.5752236581183574 1107 88-89 0.5043746345616071 1107 90-91 0.2938971883896322 1107 92-93 0.42561037906676447 1107 94-95 0.6061958751548282 1107 96-97 0.6211426870274046 1107 98-99 0.6113132388663693 1107 100-101 1.0783484435393689 1108 1 0.00307296508377064 1108 2 -0.011610446266921315 1108 3 0.035866701287964986 1108 4 0.15086503584660704 1108 5 0.1960035109328473 1108 6 0.20558883570272002 1108 7 0.016149518680109054 1108 8 0.15185463334796623 1108 9 -0.20454138958604062 1108 10-11 -0.014938356056845237 1108 12-13 -0.1845321859140796 1108 14-15 0.27525905827202024 1108 16-17 0.2315085133007031 1108 18-19 0.21414835917926212 1108 20-21 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2316 72-73 -1.4633631513579957 2316 74-75 -1.497438952385668 2316 76-77 -1.8251813674409973 2316 78-79 -1.7001138822305535 2316 80-81 -2.1580045214840133 2316 82-83 -2.108922617645028 2316 84-85 -1.9491420373809838 2316 86-87 -1.76909327565971 2316 88-89 -1.9947101360208848 2316 90-91 -1.9482572616591831 2316 92-93 -2.1798647266930793 2316 94-95 -2.4769145828666517 2316 96-97 -2.3244952278766 2316 98-99 -2.612701872588236 2316 100-101 -3.168590622519858 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 930.0 3 191.0 4 167.0 5 141.0 6 194.0 7 241.0 8 340.0 9 378.0 10 543.0 11 824.0 12 1193.0 13 1827.0 14 2078.0 15 2760.0 16 3897.0 17 5702.0 18 4617.0 19 2334.0 20 1992.0 21 1978.0 22 2194.0 23 2437.0 24 2859.0 25 3226.0 26 3793.0 27 4590.0 28 5551.0 29 6713.0 30 8340.0 31 10291.0 32 13287.0 33 17972.0 34 25361.0 35 39642.0 36 52216.0 37 82877.0 38 94289.0 39 17382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.34639352785221 25.37363291404267 16.80871735637511 19.471256201730014 2 13.778367642767723 25.081184875610653 40.719274466062366 20.42117301555926 3 15.796258960309972 31.019669747735072 34.423117188033004 18.76095410392195 4 10.117937853107344 21.807674199623353 43.281308851224104 24.793079096045197 5 9.440791486437512 41.22380107407948 37.63655447352691 11.698852965956105 6 22.459358203194864 44.324739017066264 19.66879406587407 13.547108713864805 7 21.364369182249316 38.15761912046789 25.702954917212896 14.775056780069901 8 22.03870038615672 36.01376139232911 23.044449203095844 18.903089018418335 9 23.77260434540075 19.283863268865492 25.837269896413666 31.106262489320084 10-11 20.286933713652296 30.208175210310973 31.018706468364215 18.486184607672513 12-13 22.346280639749256 30.159725539379593 30.229376039757263 17.264617781113888 14-15 20.456818390393472 31.050261082781716 29.787204002249617 18.705716524575198 16-17 16.375859301638705 33.92055710752185 31.011698975545354 18.6918846152941 18-19 16.039587186401434 32.6744881007845 34.53196105283468 16.753963659979387 20-21 17.237886742557475 31.28654798777999 34.31672981751151 17.15883545215103 22-23 17.51643382474038 31.030519624127724 34.041897430347404 17.411149120784494 24-25 17.627685626707006 32.06614175451143 33.953520102943635 16.352652515837935 26-27 16.711085915373687 31.446475785432682 35.32565229255074 16.516786006642892 28-29 16.29735928953714 31.399635358466156 35.47421043345292 16.828794918543785 30-31 17.02188215203897 31.115441700518183 34.71130754267408 17.15136860476877 32-33 16.773259506325005 31.880001317219346 34.773297141163575 16.573442035292068 34-35 17.459070472525674 31.456551370511455 34.3811558938402 16.703222263122676 36-37 17.7357913616183 30.847902149304634 34.0443974008409 17.37190908823616 38-39 16.530765620975455 32.51663332357151 33.94750491325503 17.00509614219801 40-41 16.266978465418454 32.05355472926795 34.95008357633796 16.72938322897564 42-43 16.561607887465758 31.931203457235647 33.63370851805369 17.873480137244915 44-45 16.93230652707245 31.587582355805306 33.46027280617777 18.019838310944472 46-47 17.871653364598192 32.22800925245394 32.784003179796066 17.11633420315181 48-49 17.28966961733095 32.11190650075208 32.77330689613403 17.825116985782948 50-51 16.562604356271724 32.20017120307789 33.101809390771024 18.13541504987936 52-53 17.102392799141054 33.092881343175634 32.32154554103462 17.483180316648696 54-55 17.449658115034236 32.058036942863936 32.134364202162566 18.35794073993927 56-57 17.66961995391884 32.78607544466909 31.681327250337848 17.86297735107423 58-59 17.69466007998118 31.59774170783345 32.491060926840746 18.216537285344625 60-61 17.866871156985525 32.71272717008294 32.18723095958337 17.233170713348166 62-63 17.223050168026603 32.3563834229436 32.89769138388756 17.522875025142238 64-65 17.46115372810138 32.5331426642786 32.31052556686216 17.69517804075786 66-67 17.379091970909382 32.381015113430124 31.30217332748353 18.937719588176964 68-69 16.99759828563229 31.730857442609377 32.57239839760252 18.69914587415581 70-71 17.99849730325902 32.10021270566545 32.34243142917278 17.558858561902756 72-73 17.906079881482874 31.05372070875122 32.922834534573376 18.117364875192532 74-75 17.06959724188534 33.01365417268319 31.70326750043507 18.213481084996403 76-77 17.194346638818526 32.21105787662614 32.22340608190271 18.37118940265263 78-79 17.156339795787545 32.0000282288845 32.229035054393535 18.614596920934424 80-81 17.52347466147686 32.2161138778318 31.533599487291053 18.726811973400284 82-83 17.527978875434457 32.12463023188798 31.958668305506382 18.388722587171177 84-85 17.023966914930156 31.821325567061727 31.652300946161482 19.502406571846638 86-87 17.001996097200642 32.367710040121906 31.806050397631502 18.82424346504595 88-89 17.846625543114435 31.655997524586482 31.629172701271475 18.86820423102761 90-91 17.402253693422416 31.119671591229885 32.307565634704055 19.170509080643644 92-93 18.202393373932622 31.85707042843346 32.00375957096661 17.93677662666731 94-95 17.700574709262995 30.881828716639507 32.32454308554756 19.093053488549934 96-97 17.874038073523476 30.919414061626828 31.803662879549922 19.402884985299774 98-99 17.749786396706625 31.27870110367586 31.346842319578773 19.62467018003874 100-101 17.49152701939371 31.052414799472793 31.9590237243457 19.497034456787798 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 4906.0 1 2960.5 2 941.5 3 836.5 4 716.5 5 736.0 6 1183.5 7 2049.5 8 2859.5 9 2913.5 10 2190.0 11 1550.0 12 1453.0 13 1434.5 14 1335.5 15 1175.0 16 1075.0 17 1072.5 18 1032.0 19 1067.5 20 1204.5 21 1433.0 22 1632.0 23 1820.0 24 2009.5 25 2574.5 26 3993.5 27 5578.5 28 5839.5 29 6222.0 30 8314.5 31 10407.0 32 12987.0 33 16801.5 34 20937.0 35 23421.5 36 26344.5 37 29933.0 38 28505.5 39 24836.5 40 21744.5 41 18664.0 42 16004.0 43 14714.5 44 14096.5 45 13056.0 46 11175.0 47 10396.5 48 13731.0 49 12176.0 50 5957.5 51 2609.0 52 1213.5 53 806.5 54 550.5 55 420.5 56 346.5 57 306.5 58 258.5 59 228.5 60 208.0 61 186.5 62 159.5 63 118.5 64 103.5 65 76.5 66 48.5 67 45.5 68 35.0 69 20.0 70 15.5 71 8.5 72 6.0 73 6.0 74 4.5 75 6.0 76 4.0 77 2.5 78 3.5 79 2.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.06206697120233598 2 0.09192494598527791 3 0.06535840149336894 4 0.12860088351393098 5 0.09991841954921511 6 0.09756739791276299 7 0.10861719960408794 8 0.09168984382163269 9 0.11378944720428262 10-11 0.10356250308571589 12-13 0.08651759622143802 14-15 0.09074943516705185 16-17 0.10297474767660286 18-19 0.08487188107592154 20-21 0.07205881315725748 22-23 0.07264656856637051 24-25 0.06112656254775513 26-27 0.05430859980204397 28-29 0.06394778851149767 30-31 0.04843104571091368 32-33 0.049136352201849316 34-35 0.045962472992638954 36-37 0.04925390328367192 38-39 0.05207512924741447 40-41 0.06735676988435324 42-43 0.06288982877509422 44-45 0.04937145436549453 46-47 0.03820410159234695 48-49 0.04431675784712247 50-51 0.028447361801070655 52-53 0.04126042971973471 54-55 0.047960841383623255 56-57 0.05348574222928574 58-59 0.05807023442036737 60-61 0.0528979868201727 62-63 0.06241962444780379 64-65 0.04173063404702513 66-67 0.06147921579322294 68-69 0.05501390629297962 70-71 0.02621389124644114 72-73 0.021629399055359506 74-75 0.030328179110232355 76-77 0.04325879811071901 78-79 0.05889309199312562 80-81 0.03632328428318526 82-83 0.0586579898294804 84-85 0.061361664711400345 86-87 0.06288982877509422 88-89 0.08640004513961541 90-91 0.06277227769327161 92-93 0.0701779958480958 94-95 0.0821682061940016 96-97 0.08310861484858245 98-99 0.11614046884073474 100-101 0.10979271042231402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 425347.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 18.42918840382088 #Duplication Level Percentage of deduplicated Percentage of total 1 70.2275858549778 12.942374108668922 2 9.645609021789049 3.5552149186428963 3 4.20344950757769 2.3239848876329208 4 2.3613308159412156 1.7406964196291497 5 1.6852069194264427 1.5528497908766254 6 1.1991631372148799 1.3259762029589959 7 0.960606215237026 1.2392235045739126 8 0.8253814359340715 1.2168887990276176 9 0.6365770270959841 1.0558438169306472 >10 6.3887329693320405 25.428414917702487 >50 1.0932795836097362 14.079328172057167 >100 0.7386334643057612 24.74873456260418 >500 0.02423840383732204 2.920674178964469 >1k 0.008929938255855488 4.6458538557930344 >5k 0.0012757054651222127 1.2239418639369737 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 5206 1.2239418639369737 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4708 1.106860986441658 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3662 0.8609441232687665 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3593 0.8447220739772467 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2549 0.5992754151316454 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2478 0.5825831615128354 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1649 0.38768346785095464 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1122 0.2637846276099279 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 839 0.19725071529833288 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 825 0.19395928500729992 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 782 0.18384989197055582 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 765 0.1798531551885872 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 723 0.16997886431548828 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 717 0.16856825133361703 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 652 0.15328661069667823 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 646 0.15187599771480698 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 644 0.15140579338751653 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 634 0.14905477175106444 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 623 0.1464686479509671 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 620 0.14576334146003148 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 617 0.1450580349690958 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 611 0.14364742198722455 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 609 0.14317721765993413 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 583 0.1370645614051586 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 517 0.12154781860457461 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 512 0.12037230778634855 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 504 0.11849149047718685 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 494 0.11614046884073474 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 489 0.11496495802250867 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 475 0.11167352773147571 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 473 0.11120332340418529 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 471 0.11073311907689486 No Hit GTTATACGCGTATGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACA 471 0.11073311907689486 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 470 0.11049801691324966 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 469 0.11026291474960444 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 466 0.1095576082586688 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 462 0.10861719960408794 No Hit CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC 457 0.1074416887858619 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 445 0.10462046282211936 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 445 0.10462046282211936 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 442 0.10391515633118371 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 436 0.10250454334931244 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 432 0.1015641346947316 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 2.3510216364521202E-4 0.0 5 0.0 0.0 0.0 2.3510216364521202E-4 0.0 6 0.0 0.0 0.0 2.3510216364521202E-4 0.0 7 0.0 0.0 0.0 2.3510216364521202E-4 0.0 8 0.0 0.0 0.0 2.3510216364521202E-4 0.0 9 0.0 0.0 0.0 2.3510216364521202E-4 0.0 10-11 0.0 0.0 0.0 4.7020432729042404E-4 0.0 12-13 0.0 0.0 0.0 4.7020432729042404E-4 0.0 14-15 0.0 0.0 0.0 4.7020432729042404E-4 0.0 16-17 0.0 0.0 0.0 4.7020432729042404E-4 0.0 18-19 0.0 0.0 0.0 4.7020432729042404E-4 0.0 20-21 0.0 0.0 0.0 8.228575727582421E-4 0.0 22-23 0.0 0.0 0.0 0.0015281640636938781 0.0 24-25 0.0 0.0 0.0 0.0034089813728555743 0.0 26-27 0.0 0.0 0.0 0.0058775540911303005 0.0 28-29 0.0 0.0 0.0 0.014693885227825752 0.0 30-31 0.0 0.0 0.0 0.03361960940126532 0.0 32-33 0.0 0.0 0.0 0.055131457374802226 0.0 34-35 0.0 0.0 0.0 0.07875922482114603 0.0 36-37 0.0 0.0 0.0 0.10791189311315233 0.0 38-39 0.0 0.0 0.0 0.1331853757050126 0.0 40-41 0.0 0.0 0.0 0.15610783666042077 0.0 42-43 0.0 0.0 0.0 0.18667111793429836 0.0 44-45 0.0 0.0 0.0 0.2126499070170943 0.0 46-47 0.0 0.0 0.0 0.25943523758249143 0.0 48-49 0.0 0.0 0.0 0.3157422057755197 0.0 50-51 0.0 0.0 0.0 0.3655838644683047 0.0 52-53 0.0 0.0 0.0 0.44481329361674116 0.0 54-55 0.0 0.0 0.0 0.5855219385584005 0.0 56-57 0.0 0.0 0.0 0.7921767404025419 0.0 58-59 0.0 0.0 0.0 0.9863711275734871 0.0 60-61 0.0 0.0 0.0 1.177274084453399 0.0 62-63 0.0 0.0 0.0 1.386515010097638 0.0 64-65 0.0 0.0 0.0 1.6319616689432392 0.0 66-67 0.0 0.0 0.0 1.944177342264081 0.0 68-69 0.0 0.0 0.0 2.3162265162326285 0.0 70-71 0.0 0.0 0.0 2.68568956640108 0.0 72-73 0.0 0.0 0.0 3.0475117962510607 0.0 74-75 0.0 0.0 0.0 3.478924266540025 0.0 76-77 0.0 0.0 0.0 3.9138632692836675 0.0 78-79 0.0 0.0 0.0 4.333990835717661 0.0 80-81 0.0 0.0 0.0 4.7972596491805515 0.0 82-83 0.0 0.0 0.0 5.260410911561619 0.0 84-85 0.0 0.0 0.0 5.829593249746678 0.0 86-87 0.0 0.0 0.0 6.474478484625494 0.0 88-89 0.0 0.0 0.0 7.135938422041298 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTCAAC 40 5.3759787E-4 47.514942 7 AGACAGG 40 5.3759787E-4 47.514942 6 GTCAACC 40 5.379099E-4 47.509354 8 GTATGGT 65 2.5778245E-6 43.865112 1 GCTTTGC 55 4.945373E-5 43.20049 1 ATGTATT 90 1.1890734E-8 42.24048 1 AGATCCT 70 4.2944757E-6 40.7271 5 TAAGATC 50 0.001612176 38.011955 3 GGCATTC 180 0.0 34.31635 7 CATTCCG 180 0.0 34.312313 9 GATCCTC 85 1.6262864E-5 33.539963 6 CCTTTAG 185 0.0 33.392815 1 CTTTAGG 185 0.0 33.392815 2 ATGGGTA 100 1.2934561E-6 33.26046 5 CATGGGT 160 5.2750693E-11 32.666523 4 CATATAG 120 1.496337E-7 31.68036 1 GTGGTAC 90 2.4023004E-5 31.680359 1 GGGGTCC 90 2.4042343E-5 31.67663 6 GGTACTC 110 2.7239203E-6 30.233227 8 TTGCGTT 80 4.480664E-4 29.696838 4 >>END_MODULE